This in vitro study aimed to assess whether a pre-hydrated version of Doctor's Biome Signature Probiotic Blend (DBSPB), comprising five strains of Bifidobacterium and ten strains of Lactobacillus in a fruit and vegetable juice carrier, can survive stomach acidity better than the same blend in dry powdered form. We initially mixed DBSPB with a proprietary sterilized blend of organic green juices and observed that the probiotics thrived in the juice. When exposed to a highly acidic environment (HCl at pH 1.5), the probiotics in the juice survived and formed colonies, whereas those in dry powdered form did not. These results suggest that probiotics in juice have significantly greater resistance to stomach acid than their dry counterparts. The enhanced survivability of the hydrated probiotics may be attributed to the combined effects of cellular hydration, buffering properties of the juice, and the presence of glucose. These patent-pending results, supported by visual and enumeration observations, suggest that incorporating probiotics into a fruit and vegetable juice carrier can improve their resistance to stomach acid and thereby offer a more effective delivery system for probiotic dietary supplements and medical foods.