An essential role in proper homeostasis of glucose is played by kidneys where glucose tranport is carried out across cell membranes by two families of glucose tansporters - facilitated diffusion glucose transporters (GLUTs) and Na (+) -dependent glucose co-transporters (SGLT family). Among transporters, sodium-dependent glucose co-transporters have a major role in the kidney ‘s ability to reabsorb glucose. Although localization of glucose transporters has been extensively studied in mammals there are still gaps in knowledge of localization of SGLTs in birds. The aim of the reasarch was to study comparatively immunolocalization of sodium-dependent glucose co-transporters SGLT1 and SGLT2 in kidneys of healthy and T-2 mycotoxicated chickens. Immunohistochemical staining was carried out using polyclonal primary antibodies SGLT1 and SGLT2 (Abcam, UK) on kidney tissue derived from three healthy and three T-2 mycotoxicated 7 days old female layer-type Ross chickens (Gallus gallus domesticus). Sections were stained using Immunohistochemistry kit (Abcam, UK). In kidneys of healthy birds strong staining of SGLT1 and SGLT2 was noted in cytoplasm of epithelial cells of the proximal straight and convoluted tubules. In T-2 toxin group birds' kidneys’ weak expression of SGLT1 and SGLT2 with morphological changes occurred, indicating to reduced glucose transport in urinary system during T-2 mycotoxicosis.