PRMT5 methyltranserase is an enzyme that produces monomethyl arginine (MMA) as well as symmetric dimethyl arginine (sDMA), posttranslational modifications that regulate several cellu-lar processes, including stage conversion in parasitic protozoa. Entamoeba histolytica, the etiologic agent of human amebiasis, has two stages in its life cycle, the trophozoite, which is the replicative form, and the cyst, corresponding to the infective phase. The study of the molecular mechanisms that regulate differentiation in this parasite has been overdue by the lack of efficient protocols for in vitro encystment. For this reason, Entamoeba invadens, a reptile parasite, has been used as a dif-ferentiation model system for the genus. Here, we demonstrated the presence of sDMA in E. in-vadens, which increases during encystment, and identified the PRMT5 protein of this microorgan-ism (EiPRMT5). In addition, we performed 3D modeling of this enzyme, as well as its molecular docking with the PRMT5 inhibitor EPZ015666, which predicted the affinity of the drug for the ac-tive site of the enzyme. In agreement, EPZ015666 reduced trophozoite viability and encystment. Therefore, EiPRMT5 is a potential target for the inhibition of amebiasis spread.