Mosquitoes are a family of the order Diptera, which includes more than 3500
species. Two groups
able to suck human blood and transmit diseases: the anophelines and the
culicines comprise several genera such as Culex, Aedes and Aedes communis (Ac).
Previous study has demonstrated a significant relationship between Ac sensitization
and either extract or single bee venom components suggesting a “bee-mosquito
syndrome” occurrence. It is well known that CCD can interference with the measurement of quantitative IgE results. Hence, it has been hypnotized that the presence of sIgE to CCD could have a role in the Bee-Mosquito syndrome.
Method
Serum from 21 different Mosquito and Ape melifera allergic individuals were
combined with extracts of Mosquito (in house) and Apis melifera. SDS-PAGE
and Immunoblot (IB) were carried out without and with with CCD-Inhibitor.
Results
The data would
suggest IgE binding to common bands between Culex male, Culex female and
Aedes species mosquitos of approximate molecular weight ~21kDa, ~35kDa,
~40kDa and ~55kDa. Whilst IgE binding has been observed to protein bands from
the Apis melifera extract, these bands do not align with any present in the
mosquito extracts.
The use of a CCD-Inhibitor results in differences in IgE binding. Specifically, bands that were previously observed in the Apis melifera venom sample without use of CCD-Inhibitor at 40kDa and 90kDa are no longer observed when CCD-Inhibitor is used. Two bands of the Apis melifera venom remain present between 15-20kDa.
No major differences in banding pattern were observed for the mosquito samples with or without CCD-Inhibitor, although the blot appears cleaner with less background when CCD-Inhibitor is used.
Conclusion
Further experiments have to be done to determine whether the reactive bands
from Apis melifera are unrelated proteins or whether the proteins are related
homologues of varying molecular weight.