Carob pulp is a valuable source of cellulose-rich fraction (CRF) for many food applications. This study aimed to obtain and characterize a CRF derived from carob pulp waste, after sugar removal, and to evaluate its potential use in the 3D printing of cellulose-rich foods. Thus, extraction of the CRF present in carob pulp (by obtaining the alcohol-insoluble residue) was carried out, accounting for nearly 45 % dm (dry matter) of this by-product. The CRF contained about 24 % dm of cellulose. The functional properties (swelling capacity, water retention and fat adsorption) related to this fraction were determined, which showed a value of 5.9 mL/g of CRF, 4.0 and 6.5 g/g of CRF, respectively. Different gels were formulated with a total solids content of 15 % wm (wet matter), using potato peel flour as a base and partially substituting with CRF (0-8 % wm). The cellulose-based gels were characterized in terms of viscosity, water distribution (low-field NMR), and printability, while the 3D-printed samples were assessed for their textural properties. As the percentage of added CRF increased, the viscosity decreased while the water retention increased. Printability improved when small proportions of CRF (2-4%) were used, while deteriorated for higher percentages (6-8 %). The textural properties (hardness, adhesiveness, cohesiveness, and gumminess) showed significant changes caused by the addition of CRF, with gels containing 3-4 % CRF exhibiting the most suitable printing values. In summary, CRF extracted from carob pulp waste can be used as an ingredient in the 3D printing of novel cellulose-rich foods, reducing food waste within the framework of the circular economy.

This study concentrates on assessing the insecticidal attributes of γ-Al2O3 nanoparticles derived from remnants of Mentha pulegium, which include essential oil, ethanolic extract, and plant waste. The synthesis of γ-Al2O3 nanoparticles was executed via a direct sol-gel procedure, affirming the crystal structure through extensive physicochemical analyses such as UV-VIS, XRD, FTIR, and SEM. The evaluation of insecticidal activity in vitro was conducted against Xylosandrus crassiusculus, a pest that infests carob wood, utilizing strains from diverse forests in the Khenifra region, situated in the Moroccan Middle Atlas. The lethal doses 50 ranged from 40 mg/g to 68 mg/g, indicating moderate effectiveness compared to the commercial insecticide Permethrin. The optimization of the conditions for the efficiency of γ-Al2O3 nanoparticles was determined by experimental plans, revealing that time, humidity, and temperature were influential factors in the lethal dose 50 of these nanomaterials. Moreover, this study encompasses the establishment of correlations through Principal Component Analysis (PCA) and Ascending Hierarchical Classification (AHC) among various geographic, biological, and physical data, amalgamating geographic altitude, γ-Al2O3 nanoparticle insecticide parameters, as well as the attributes of mechanical tests conducted on carob wood affected by insects. The correlation highlights a close connection between the effectiveness of the insecticide, mountain altitude, and the mechanical parameters that were examined. Ultimately, these nanoparticles demonstrate promising potential as alternative insecticides, thus opening encouraging prospects for safeguarding against carob wood pests.

For the development of a high-quality functional bread it is of crucial importance to find a compromise between improving the functional value of the product and impairing its quality. Therefore, the primary aim of this work was to define the effects of carob pulp flour (CF; 10-20% on flour basis) on rheological behavior of dough and quality parameters of bread. Introduction of CF caused a major increase in dough resistance while significantly decreasing dough extensibility, resulting in lower volume with denser crumb structure of obtained bread samples. The negative effects of CF on dough and bread characteristics were partialy compensated by the addition of dry sourdough (DS) as an improver in the amount of 5-10% (flour basis). It was determined that by combining 15% CF and 10% DS bread of very good sensory quality can be produced. The content of total dietary fiber (6.48 g), Mn (0.76 mg) and Cu (0.21 mg) per 100 g of mentioned bread was sufficient for the nutritional claims: "high in fiber", "high in manganese" and "source of copper". Obtained bread had 10 times higher content of total phenols and multiple times higher antioxidant activity compared to the control. Moreover, microbiological tests confirmed the durability of bread with 15% CF and 10% DS up to seven days. Therefore, carob pulp flour can be successfully applied in the production of bread, which characteristics are adapted to modern trends in nutrition.

Ceratonia siliqua L. is a medicinal plant that has long been used in traditional Moroccan medicine to treat many diseases. This study aimed to assess the impact of the stages of the immature phase of carob pulp (M1, M2, M3, M4, and M5) on phytochemical composition, antioxidant activity, and antidiabetic activity of Ceratonia siliqua L. The identification of the phenolic profile by HPLC-UV/MS-MS and the study of the antidiabetic effect by in silico, in vitro, and in vivo studies were carried out for extracts with a high content of phenolic compounds from immature wild carob pulp from the communes of Timoulit (TM), Bin Elouidane (AW), and Ouaouizerth (TG) in the province of Azilal in the Beni Mellal-Khenifra region. The results revealed a gradual increase in total sugar content over the pulp's ripening period, reaching a value of 2134 ±56.23 mg GE/100 g fresh matter (FM) for TG. The three locations showed peak values for total polyphenol content (TPC), total flavonoid content (TFC), and total condensed tannin (TCT) at the M2 stage. AW has the highest concentrations of TPC (3819 ± 226.4 mg GAE/100 g FM), TFC (1034 ± 57.08 mg QE/100 g FM), and TCT (1472 ± 28.46 mg CE/100 g FM). The DPPH assay (7892 ± 296.1 mg TE/100 g FM) and the FRAP assay (278.2 ± 7.85 mg TE/100 g FM) both demonstrate that the TG zone is a highly potent antioxidant zone. In contrast, the AW site exhibits a markedly elevated value of 725.4 ± 103.6 mg TE/100 g FM in the ABTS assay. HPLC-UV-MS/MS analysis showed that the methanolic extracts of immature carob pulp (MEICP) from the three areas contained several different chemical compounds. The most prevalent were 3-O-p-coumaroyl-5-O-caffeoylquinic acid, quercetin 3-methyl ether, gallic acid, and galloylquinic acid. Immature carob pulp extract. (ICPE) from AW showed the strongest in vitro inhibition of pancreatic α-amylase (IC50 = 0.405 µg/mL) and TG extracts were most potent against intestinal α-glucosidase (IC50 = 0.063 µg/mL). In vivo, AW, TG, and TM extracts significantly reduced postprandial glycemia in rats, with AW having the greatest effect. These results highlight the antidiabetic potential of ICPE. The 3-O-p-Coumaroyl-5-O-caffeoylquinic acid showed a better affinity for α-amylase compared to acarbose and interacted significantly with several amino acid residues of the enzyme. Similarly, this molecule and 3,4-Dicaffeoylquinic acid demonstrated a strong affinity for α-glucosidase, suggesting their potential as natural inhibitors of enzymes involved in carbohydrate metabolism. Most of the compounds are not substrates of P-glycoprotein and exhibit high intestinal absorption. Furthermore, the majority of these compounds do not act as inhibitors or substrates of CYP450 enzymes, reinforcing their suitability for development as oral medications. These results underscore the potential of immature carob pulp as a promising antidiabetic agent.