Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of 5-Aza-2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture

Version 1 : Received: 18 August 2016 / Approved: 19 August 2016 / Online: 19 August 2016 (05:54:39 CEST)
Version 2 : Received: 27 December 2016 / Approved: 27 December 2016 / Online: 27 December 2016 (09:36:54 CET)

How to cite: Haq, S. H. Effects of 5-Aza-2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture. Preprints 2016, 2016080176. https://doi.org/10.20944/preprints201608.0176.v1 Haq, S. H. Effects of 5-Aza-2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture. Preprints 2016, 2016080176. https://doi.org/10.20944/preprints201608.0176.v1

Abstract

DNA methylation is one of the epigenetic mechanisms which have been implicated in cellular differentiation, ageing and disease development. The effect of hypomethylating drug 5-aza-2’ deoxycytidine (5-aza dC) on the biosynthetic profile of caudal region chondrocytes from chick sternum was studied in detail. The chondrocytes in culture were treated with varying doses of 5-aza dC for 48h and maintained subsequently without the treatment and harvested at selected time points for analysis of growth and differentiation status. 15µg/ml of 5-aza dC showed optimum Concentration at which there was a significant increase in DNA synthesis and RNA synthesis as per cell basis. There was also a significant increase in total protein synthesis and collagen synthesis as per cell basis at this concentration. This optimal concentration also showed to up regulate the gene expression of Type X collagen and alkaline phosphatase, which are the marker of hypertrophic chondrocyte expression. These results further support the notion that methylation is the major epigenetic factor controlling the differentiation and maturation of chondrocytes

Keywords

ALP (alkaline phosphatase); OA (osteoarthritis); 5-aza dC (5-aza-2’ deoxycytidine); epigenetics

Subject

Biology and Life Sciences, Cell and Developmental Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.