Initial Multitarget Approach Shows Importance for Improved Caprine Arthritis-encephalitis Virus Control Program in Russia for Hobbyist Goat Farms

Version 1 : Received: 10 September 2020 / Approved: 12 September 2020 / Online: 12 September 2020 (09:57:24 CEST)
Version 2 : Received: 20 January 2021 / Approved: 21 January 2021 / Online: 21 January 2021 (12:46:50 CET)

A peer-reviewed article of this Preprint also exists.

Shuralev EA, Khammadov NI, Osyanin KA, Elizarova IA, Salmanova GR, Shamaev ND, Petrov SV, Whelan C, Saushkin NY, Samsonova JV, Galimzyanov IG, Efimova MA, Khaertynov KS, Faizov TK, Mukminov MN, Ivanov AV (2021) Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms, Veterinary World, 14(7): 1718-1726. Shuralev EA, Khammadov NI, Osyanin KA, Elizarova IA, Salmanova GR, Shamaev ND, Petrov SV, Whelan C, Saushkin NY, Samsonova JV, Galimzyanov IG, Efimova MA, Khaertynov KS, Faizov TK, Mukminov MN, Ivanov AV (2021) Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms, Veterinary World, 14(7): 1718-1726.

Abstract

Aim: The aim of this study was to use a multi target approach to testing with both serological tests and an in-house real-time molecular test to investigate the prevalence of the caprine arthritis-encephalitis virus (CAEV) in goats from three hobbyist farms in the Republic of Tatarstan, Russia. Materials and Methods: We have approached the detection of using a multi target approach testing with both ELISA and an in-house real-time PCR test to investigate the prevalence of CAEV in goats. Animals from three hobbyist farms were used in this study. The animals from two farms (n=13 for F1 and n=8 for F2) had clinical signs of arthritis and mastitis. In the third farm (n=15 for F3), all goats were homebred and had no contact with imported animals. Results: CAEV antibodies (ELISA targets TM env and gag genes) were detected in serum samples from two farms (F1 and F2), indicating a seroprevalence 87.50-92.31%. Specific CAEV antibodies were also detected in milk samples. CAEV proviral DNA was detected in 53.85-62.50%. Results from all tests performed in the third farm (F3) were negative, indicting all tests were 100% specific.Conclusion: Results of this work show that CAEV is circulating and present in small hobbyist goat farms in Russia. Serological and molecular tests could be of importance for CAEV control and eradication programs in Russia for hobbyist goat farms.

Keywords

caprine arthritis-encephalitis virus; goat; antigens; antibodies; proviral DNA

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Received: 21 January 2021
Commenter: Eduard A. Shuralev
Commenter's Conflict of Interests: Author

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