Maize varieties with high amylose proportion are more valuable for starch industry. The SBEⅡb gene encodes one of the starch branching isozymes (SBEⅠ, SBEⅡa, and SBEⅡb). Its recessive mutant amylose-extender (ae/sbe2b) decreases the total activities of SBEs and increases amylose proportion up to 60%. Here, the breeding potential of introduced germplasm line GEMS-0067 was evaluated by genotyping and phenotyping. The deletion of the ninth exon of the SBEⅡb gene, high amylose proportion, and the typical irregular granules suggested that this germplasm line was derived from the same resource of high amylose line AE11. The gelatinization and thermal properties, and degree of polymerization of starch chain showed its advantages used for high amylose breeding. However, the negative correlation between amylose proportion and starch content, as well as ker-nel filling characteristics should be overcome during breeding process.