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Resolving Relationships of Bolbitis sinensis Species Complex Using RAD Sequencing
Version 1
: Received: 1 June 2024 / Approved: 3 June 2024 / Online: 3 June 2024 (12:10:43 CEST)
A peer-reviewed article of this Preprint also exists.
Nie, L.; Fang, Y.; Xia, Z.; Wei, X.; Wu, Z.; Yan, Y.; Wang, F. Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing. Plants 2024, 13, 1987. Nie, L.; Fang, Y.; Xia, Z.; Wei, X.; Wu, Z.; Yan, Y.; Wang, F. Relationships Within Bolbitis sinensis Species Complex Using RAD Sequencing. Plants 2024, 13, 1987.
Abstract
Species identification and phylogenetic relationships clarification are fundamental goals in species delimitation. Yet these tasks pose challenges when based on morphologies, geographic distribution, and genomic data. Previously, two new species of fern genus Bolbitis, B. × multipinna and B. longiaurita, were described based on morphological traits, are phylogenetically intertwined with B. sinensis and fail to form monophyletic groups. To address the unclear phylogenetic relationships within the B. sinensis species complex, RAD sequencing was performed on 65 individuals from five populations. Our integrated analysis of phylogenetic tree, neighbor net, and genetic structure, indicate that the B. sinensis species complex should not be considered as separate species. Moreover, our findings reveal differences in the degree of genetic differentiation among five populations, ranged from low to moderate, which might be influenced by geographical distance and gene flow. The Fst values also confirmed that genetic differentiation intensifies with increasing geographic distance. Collectively, this study clarifies the complex phylogenetic relationships within the B. sinensis species complex, elucidates the genetic diversity and differentiation across the studied populations, and offers valuable genetic insights that contribute to the broader study of evolutionary relationships and population genetics within the Bolbitis species.
Keywords
Bolbitis; Species delimitation; Phylogenetic analysis; Genetic diversity and differentiation; RAD sequencing
Subject
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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