PreprintArticleVersion 1This version is not peer-reviewed
Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission
Version 1
: Received: 27 June 2024 / Approved: 28 June 2024 / Online: 28 June 2024 (12:10:07 CEST)
How to cite:
Maia, L. J.; Silva, A. B.; Oliveira, C. H. D.; Campos, F. S.; Silva, L. A. D.; Abreu, F. V. S. D.; Ribeiro, B. M. Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Preprints2024, 2024061993. https://doi.org/10.20944/preprints202406.1993.v1
Maia, L. J.; Silva, A. B.; Oliveira, C. H. D.; Campos, F. S.; Silva, L. A. D.; Abreu, F. V. S. D.; Ribeiro, B. M. Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Preprints 2024, 2024061993. https://doi.org/10.20944/preprints202406.1993.v1
Maia, L. J.; Silva, A. B.; Oliveira, C. H. D.; Campos, F. S.; Silva, L. A. D.; Abreu, F. V. S. D.; Ribeiro, B. M. Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Preprints2024, 2024061993. https://doi.org/10.20944/preprints202406.1993.v1
APA Style
Maia, L. J., Silva, A. B., Oliveira, C. H. D., Campos, F. S., Silva, L. A. D., Abreu, F. V. S. D., & Ribeiro, B. M. (2024). Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Preprints. https://doi.org/10.20944/preprints202406.1993.v1
Chicago/Turabian Style
Maia, L. J., Filipe Vieira Santos de Abreu and Bergmann Morais Ribeiro. 2024 "Characterization of Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission" Preprints. https://doi.org/10.20944/preprints202406.1993.v1
Abstract
Studies on animal virome have mainly concentrated on chordates and medically significant invertebrates, often overlooking sylvatic mosquitoes, constituting the major part of mosquito species diversity. Despite their potential role in arbovirus transmission, the viromes of sylvatic mosquitoes remain largely unexplored. These mosquitoes may also harbor insect-specific viruses (ISVs), affecting arboviral transmission dynamics. The Cerrado biome, known for rapid deforestation and its status as a biodiversity hotspot, offers an ideal setting for investigating mosquito viromes due to potential zoonotic spillover risks from land use changes. This study aimed to characterize the viromes of sylvatic mosquitoes collected from various locations within Minas Gerais state, Brazil. Total RNA was extracted from mosquito pools of Psorophora albipes, Sabethes albiprivus, Sa. chloropterus, Psorophora ferox, and Coquillettidia venezuelensis species, followed by high-throughput sequencing (HTS). Bioinformatic analysis included quality control, contig assembly, and viral detection. Sequencing data analysis revealed 11 near-complete viral genomes (new viruses are indicated with asterisks) across seven viral families and one unassigned genus. These included: Xinmoviridae (Ferox mosquito mononega-like virus* and Albipes mosquito Gordis-like virus*), Phasmaviridae (Sabethes albiprivus phasmavirus*), Lispiviridae (Pedras lispivirus isolate MG), Iflaviridae (Sabethes albiprivus iflavivirus*), Virgaviridae (Buriti virga-like virus isolate MG and Sabethes albiprivus virgavirus 1*), Flaviviridae (Psorophora ferox flavivirus*), Mesoniviridae (Alphamesonivirus cavallyense isolate MG), and the genus Negevirus (Biggie virus isolate MG virus and Coquillettidia venezuelensis negevirus*). Phylogenetic analyses revealed evolutionary relationships with varying degrees of similarity to known sequences in distinct host species. In some cases, new viruses and potential new genera were discovered, emphasizing the importance of studying sylvatic mosquito virome to understand viral diversity and evolution in ecologically sensitive regions. Moreover, the presence of ISVs and potential novel arboviruses underscores the need for ongoing surveillance and control strategies to mitigate the risk of emerging infectious diseases.
Copyright:
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