In this study, bioactive compounds present in chestnut shells (CS) - derived from the industrial peeling processing - were extracted through different procedures. The aqueous extracts were characterized and the highest extraction yield and total phenolic content was obtained by Conventional Liquid Extraction (CLE). Gallic and protocatechuic acids were the main simple phenols in the extract with 86.97 and 11.20 mg/g chestnut shells dry extract (CSDE), respectively. Six tumor cell lines (DU 145, PC-3, LNCaP, MDA-MB-231, MCF-7 and Hep G2) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of CSDE (1-100 µg/mL) for 24 h and cell viability was evaluated using MTT assay. A reduced rate in cell viability was observed in DU 145, PC-3, LNCaP and MCF-7 cells while viability of MDA-MB-231 and Hep G2 cells was not affected except for PNT2 cells at a concentration of 100 µg/mL. Furthermore, CSDE-at concentrations of 55.5 and 100 µg/mL- lead to a significant increase of apoptotic cells in DU 145 cells of 28.2% and 61%, respectively. In conclusion, our findings showed that CS might be used for the extraction of several polyphenols that may represent good candidates for alternative therapies or in combination with current chemotherapeutics.

As education becomes more and more important in creating improved societies, many people who do not have access to it are falling behind. To help them catch up, many people, especially those in rural areas and developing countries, are turning to different methods of self-learning, especially those that utilize cheap technology and use interactive methods to teach. Our empirical study tests the effectiveness of an e-learning system that utilizes newer, less tested forms of interactivity and could potentially be used in these areas as a self-learning system and compares it to non-interactive video and textbook self-learning in two different topics. The results of the experiment showed that the increased interactivity provided by the e-learning system achieved significantly better learning performance over both non-interactive video and textbook self-learning. It was also found that students who learned through non-interactive video performed significantly better than those who used textbooks for self-learning.

Urolithiasis is a painful experience associated with hematuria, damage to kidney tissue and renal failure. It is a multi-factorial disorder while in India, its prevalence is rapidly increasing imposing a large burden for both healthcare and economy globally. In this article, we aimed to evaluate the association between genetic defects in SPP1 gene and urolithiasis from East Indian patients.75 urolithiasis patients were recruited from SSKM Hospital & Institute of Post Graduate Medical Education & Research (IPGME&R), Kolkata, India while 75 healthy controls were recruited from the same community. SNPs based areas of SPP1 gene were analyzed by direct sequencing to identify genetic defects.We identified 3 polymorphisms one synonymous and two 3’UTR variants rs1126616: p.Ala250Ala, rs1126772: 7315 a>g, rs9138: 7471 a>c in SPP1 gene in study individuals. Genotype and allele frequency analysis of these SNPs revealed that, rs9138 SNP was significantly associated with urolithiasis risk in East Indian patients. To our knowledge this is the first study reporting the role of the gene with urolithiasis in the population of West Bengal, India.

Human African Trypanosomiasis (HAT) commonly known as sleeping sickness, is a neglected tropical vector borne disease caused by trypanosome protozoa. It is transmitted by bites of infected tsetse fly. In this paper we first present the vector-host model which describes the general transmission dynamics of HAT. In the tsetse fly population, the HAT is modelled by three compartments while in the human population, the HAT is modelled by four compartments. The next generation matrix approach is used to derive the basic reproduction number, R₀, and also it is proved that if R₀ ≤ 1 the disease free equilibrium is globally asymptotically stable, which means the disease dies out. The disease persist in the population if the value of R₀ > 1. Furthermore, the optimal control model is determined by using the Pontryagin’s maximum principle with control measures such as education, treatment and insecticides used to optimize the objective function. The model simulations confirm that the use of the three control measures are very efficient and effective to eliminate HAT in Africa.

We introduce tools to capture the dynamics of three different pathways, in which the synchronization of human decision making could lead to turbulent periods and contagion phenomena in financial markets. The first pathway is caused when stock market indices, seen as a set of coupled integrate-and-fire oscillators, synchronize in frequency. The integrate-and-fire dynamics happens due to "change blindness", a trait in human decision making where people have the tendency to ignore small changes, but take action when a large change happens. The second pathway happens due to feedback mechanisms between market performance and the use of certain (decoupled) trading strategies. The third pathway occurs through the effects of communication and its impact on human decision making. A model is introduced in which financial market performance has an impact on decision making through communication between people. Conversely, the sentiment created via communication has an impact on financial market performance.

Emergence of more virulent forms of human pathogenic bacteria with multi drug resistance is a serious global issue and requires alternative control strategies. The current study was focused to investigate the antibacterial and antibiofilm potential of ferulic acid grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The present result showed that CFA at 64 µg/mL concentration exhibit bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log CFU) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to the altered membrane integrity and permeability. CFA dose-dependently inhibited biofilm formation (52-89% range), its metabolic activity (30.8-75.1% range) and eradicated mature biofilms, and reduced viability (71-82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-MIC concentration of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will insight a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.

In The Work of the Dead: A Cultural History of Mortal Remains, Thomas Laqueur argues that the work of the dead is carried out through the living and through those who remember, honour, and mourn the dead. Further, he maintains that the brutal or careless disposal of the corpse “is an attack of extreme violence”. To treat the dead body as if it does not matter or as if it were ordinary organic matter would be to deny its humanity. From Laqueur’s point of view it is inferred that the dead are believed to have rights and dignities that are upheld through rituals, practices, and beliefs of the living. Drawing on dark tourism scholarship and cultural memory theory, this paper examines the display of human bones at Sedlec Ossuary, Czech Republic and the tourist culture that has built up around the site. Primarily, my writing calls into question the commoditization of burial places as a conceivable violation of the human rights of the dead. My research is driven by a number of questions: What is it that draws tourists to burial grounds and how do heritage sites negotiate visitor experiences? What are the ethical boundaries when a final resting place with bodies on display is also marketed as a tourist site? Do the dead have human rights and how are the living responsible for preserving those rights?

Epstein–Barr virus (EBV) has been recently shown to be co-present with high-risk human papillomaviruses (HPVs) in human cervical cancer; thus, these oncoviruses play an important role in the initiation and/or progression of this cancer. Accordingly, our group has recently viewed the presence and genotyping distribution of high-risk HPVs in cervical cancer in Syrian women; our data pointed out that HPVs are present in 95.45% of our samples. Herein, we aim to explore the co-prevalence of EBV and high-risk HPVs in 44 cervical cancer tissues from Syrian women using polymerase chain reaction (PCR), immunohistochemistry (IHC) and tissue microarray (TMA) analyses. We found that EBV and high-risk HPVs are co-present in 15/44 (34%) of the samples. Additionally, we report that the co-expression of LMP1 and E6 genes of EBV and high-risk HPVs, respectively, is associated with poorly differentiated squamous cell carcinomas phenotype; this is accompanied by a strong and diffused Id-1 overexpression, which is an important regulator of cell invasion and metastasis. These data imply that EBV and HPVs are co-present in cervical cancer in the Middle East area including Syria and their co-presence is associated with a more aggressive cancer phenotype. Future investigations are needed to elucidate the exact role of EBV and HPVs cooperation in cervical carcinogenesis.

Significant changes have occurred at Arabian Gulf Coast of Saudi Arabia over the last three decades. The area of mangrove was reduced by about 55%. Thus the economic, social, and environmental value of mangroves must be assessed over short -to long-term scales and employ these assessments for the awareness rising at local communities. This study provides a preliminary assessment of the risks on mangrove vegetation; it will provide database to mitigate the tremendous pressures due to coastal development and urban activities. The effects of human development on the mangrove plant cover in Eastern Region of KSA were recognised, during 2013 to 2016. The mean variations of physio-chemical characteristics in water and sediment were recorded. With regard to water analyses including; nitrogen, phosphorus, TSS, TDS, BOD, and turbidity were evaluated. On the other hand, for sediment: TDS, nitrogen, phosphorus, sulphate and total organic carbon were assessed. Moreover, the growth parameters: plant height, and size index, of Avicennia marina were recorded and estimated. It is concluded that human impact and urban developments have exerted drastic effects on the coastal ecosystems and its environments.

Lung cancer is almost the most common cause of cancer death in the world. Clinically, the conventional therapy to eradicate the cancer cells is chemotherapy but a better drug remains required. In this study, the effects of three bufadienolides, kalantuboside B, kalantuboside A and bryotoxin C, isolated from Kalanchoe tubiflora (Harvey) were evaluated and characterized in CL1-5 highly metastatic human lung cancer cells. Contrary to the apoptosis-promoting activity in other cancer cells, these three bufadienolides did not induce apoptosis in CL1-5 cancer cells. Instead, they activated an autophagy pathway, as indicated by the increase of autophagosomes formation. The induction of autophagy by these three bufadienolides was demonstrated to link to down-regulation of p-mTOR as well as up-regulation of LC3-II, ATG5, ATG7, Beclin-1. Moreover, among these three compounds, kalantuboside B in which a monosaccharide is attached at the bufadienolide aglycon, exhibited a better autophagy induction. Our findings revealed an alternative mechanism of drug action by bufadienolides in lung cancer cells and provided evidence for the possibility of treating highly metastatic human lung cancer through an autophagy pathway.

Bronchopulmonary dysplasia (BPD) is the most common complication after preterm birth. Pasteurized donor human milk (DHM) has increasingly become the standard of care for very preterm infants over the use of preterm formula (PF) if mother’s own milk (MOM) is unavailable. Studies have reported beneficial effects of DHM on BPD. We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) and observational studies on the effects of DHM on BPD and other respiratory outcomes. Eighteen studies met the inclusion criteria. Meta-analysis of RCT’s could not demonstrate that supplementation of MOM with DHM reduced BPD when compared to PF (3 studies, risk ratio [RR] 0.89, 95% confidence interval [CI] 0.60–1.32). However, meta-analysis of observational studies showed that DHM supplementation reduced BPD (8 studies, RR 0.78, 95% CI 0.67–0.90). An exclusive human milk diet reduced the risk of BPD, compared to a diet with PF and/or bovine milk-based fortifier (3 studies, RR 0.80, 95% CI 0.68–0.95). Feeding raw MOM, compared to feeding pasteurized MOM, protected against BPD (2 studies, RR 0.77, 95% CI 0.62–0.96). In conclusion, our data suggest that DHM protects against BPD in very preterm infants, but pasteurization of human milk reduces the benefit.

Currently there is little doubt left on the symbiogenetic nature of eukaryotes - genomes of archaeon and bacterium participated in shaping a genome of last eukaryotic common ancestor in equal albeit asymmetric manner, while a merger event itself indicated the advent of a new domain of life. The “symbiogenetic” framework of interaction of two partners is proposed, outlining similar steps essential for three major advents: the origin of life, the origin of complex life and the origin of humans. Given the immense importance of proper energy source for the evolution of life it seems plausible that for any principal increase in complexity, a partnership with a novel energy donor is required. Moreover, a “language” elaborated in the course of communication of partners might have been a prerequisite for a subsequent increase in complexity. Transitions, which led to RNA-protein world, eukaryotes and human brain, resulted in advent of complex languages via communication onsets between two entities in close partnership. Accordingly this further facilitated formation of first cells, multicellular organisms and human society.

We report the characterization of phenotype, genotype, and functional parameters such as CD4+ T cell, viral load, HIV-1 genotype, class I HLA genotype, CCR5 polymorphism, and IFN-γ producing cells from a cohort of 37 people living with HIV-1 (PLWH) (viremic controllers, were compared those on antiretroviral therapy (ART) from two sites in São Paulo, Brazil. Half the PLWH (51.4%) were classified as viremic controllers (Controller group - CG), compared to 48.6% were on ART. As expected, controllers showed baseline higher levels of CD4+ T cells and nadir compared to those under ART, confirming the ability of HIV-1 controllers to preserve these cells even in the absence of ART. IFN-γ-producing cells showed greater magnitude to the HIV peptides Gag in controllers (p<0.03). Our results demonstrated that HLA protective alleles are associated with strong production of IFN-γ to Gag, Nef and RT epitopes in people living with HIV controllers.

The idea of developing a robot controlled by iris movement to assist physically disabled individuals is indeed innovative and has the potential to significantly improve their quality of life. This technology can empower individuals with limited mobility and enhance their ability to interact with their environment. Disability of movement has huge impact on the life of physically disabled people. Therefore, there is need to develop a robot which can be controlled using iris movement. The main idea of this work revolves around iris recognition from an eye image, specifically identifying the centroid of the iris. The centroid's position is then utilized to issue commands to control the robot. This innovative approach leverages iris movement as a means of communication and control, offering a potential breakthrough in assisting individuals with physical disabilities. The proposed method aims to improve the precision and effectiveness of iris recognition by incorporating advanced segmentation techniques and fuzzy clustering methods. The fast gradient filters using a fuzzy inference system (FIS) is employed to separate the iris from its surroundings. Then, the bald eagle search (BES) algorithm is employed to locate and isolate the iris region. Subsequently, the Fuzzy KNN algorithm is applied for the matching process. This combined methodology aims to improve the overall performance of iris recognition systems by leveraging advanced segmentation, search, and classification techniques. The results of the proposed model are validated using True Success Rate (TSR) and compared to other existing models. The results highlight the effectiveness of the proposed method for the 400 tested images representing 40 people.

The paper delineates the developed vision system of the UR5 cobot and the operating algorithm of the robotic quality control station. The hardware-software architecture of the developed robotic station was shown, consisting of a UR5 cobot equipped with a web camera and a stationary industrial camera with a lighting system. Image processing and analysis algorithms were described, the method of control and communication between the station components was discussed, and two operating scenarios were presented as a single robotic station and a robotic line. Based on the results obtained, the level of measurement noise, accuracy, and repeatability of the developed vision system were estimated.

This paper presents a two-dimensional mathematical model of compound eye vision. Such a model is useful for solving navigation issues for autonomous mobile robots on the ground plane. The model is inspired by the insect compound eye that consists of ommatidia, which are tiny independent photoreception units, each of which combines a cornea, lens, and rhabdom. The model describes the planar binocular compound eye vision, focusing on measuring distance and azimuth to a circular feature with an arbitrary size. The model provides a necessary and sufficient condition for the visibility of a circular feature by each ommatidium. On this basis, an algorithm is built for generating a training data set to create two deep neural networks (DNN): the first detects the distance, and the second detects the azimuth to a circular feature. The hyperparameter tuning and the configurations of both networks are described. Experimental results showed that the proposed method could effectively and accurately detect the distance and azimuth to objects.

Parallel-serial (hybrid) manipulators represent robotic systems composed of kinematic chains with parallel and serial structures. These manipulators combine the benefits of both parallel and serial mechanisms, such as increased stiffness, high positioning accuracy, and a large workspace. This study discusses the existing architectures and applications of parallel-serial robots and the methods of their design and analysis. The paper reviews around 500 articles and presents over 150 architectures of manipulators used in machining, medicine, and pick-and-place tasks, humanoids and legged systems, haptic devices, simulators, and other applications, covering both lower mobility and kinematically redundant robots. After that, the paper considers how researchers have developed and analyzed these manipulators. In particular, it examines methods of type synthesis, mobility, kinematic, and dynamic analysis, workspace and singularity determination, performance evaluation, optimal design, control, and calibration. The review concludes with a discussion of current trends in the field of parallel-serial manipulators and potential directions for future studies.

This work is part of an ongoing research project to develop an unmanned flying social robot to monitor dependants at home in order to detect the person’s state and bring the necessary assistance. In this sense, this paper focuses on the description of a virtual reality (VR) simulation platform for the monitoring process of an avatar in a virtual home by a rotatory-wing autonomous unmanned aerial vehicle (UAV). This platform is based on a distributed architecture composed of three modules communicated through the Message Queue Telemetry Transport (MQTT) protocol: the UAV Simulator implemented in MATLAB/Simulink, the VR Visualiser developed in Unity, and the new emotion recognition (ER) System developed in Python. Using a face detection algorithm and a convolutional neural network (CNN), the ER System is able to detect the person’s face in the image captured by the UAV’s on-board camera and classify the emotion among seven possible ones (surprise, fear, happiness, sadness, disgust, anger or neutral expression). The experimental results demonstrate the correct integration of this new computer vision module within the VR platform, as well as the good performance of the designed CNN, with around 85% in the F1-score, a mean of the precision and recall of the model. The developed emotion detection system can be used in the future implementation of the assistance UAV that monitors dependent people in a real environment, since the methodology used is valid for images of real people.

Abstract: Mass spectrometry research laboratories reported multiple probes for ambient ionization in the last years. Combining them with a mechanical moving stage enables automated sampling and imaging applications. We developed a robotic platform, which is based on RepRap 3D-printer components, and therefore easy to reproduce and to adopt for custom prototypes. The minimal step width of the Open LabBot is 12.5 μm, and the sampling dimensions (x, y, z) are 18 × 15 × 20 cm. Adjustable rails in an aluminium frame construction facilitate the mounting of additional parts such as sensors, probes, or optical components. The Open LabBot uses industry-standard G-code for its control. The simple syntax facilitates the programming of the movement. We developed two programs: 1) LABI-Imaging, for direct control via a USB connection and the synchronization with MS data acquisition. 2) RmsiGUI, which integrates all steps of mass spectrometry imaging: The creation of G-code for robot control, the assembly of imzML files from raw data and the analysis of imzML files. We proved the functionality of the system by the automated sampling and classification of essential oils with a PlasmaChip probe. Further, we performed an ambient ionization mass spectrometry imaging (AIMSI) experiment of a lime slice with laser desorption low-temperature plasma (LD-LTP) ionization, demonstrating the integration of the complete workflow in RmsiGUI. The design of the Open LabBot and the software are released under open licenses to promote their use and adoption in the instrument developers’ community.

Human-Computer Interaction (HCI) has evolved significantly with advances in Artificial Intelligence (AI) and Computer Vision (CV), paving the way for more intelligent and adaptive user interfaces. This paper explores the design and implementation of user interfaces that leverage AI and CV to create more intuitive, responsive, and personalized experiences. By integrating techniques such as facial recognition, gesture control, and eye tracking, interfaces can adapt in real-time to users’ actions and intentions, thus enhancing accessibility, efficiency, and engagement across various applications, from healthcare to gaming. The study reviews current advancements and challenges in using AI and CV for HCI, including data privacy, model interpretability, and computational efficiency. Experimental results demonstrate the potential of these technologies to improve interaction quality and user satisfaction. This work aims to contribute to the growing field of intelligent HCI design, offering insights into practical implementations and future research directions in AI-driven interface development.

Plants are continuously exposed to environmental challenges, including pollutants, pesticides, and heavy metals, collectively termed xenobiotics. These substances induce oxidative stress by generating reactive oxygen species (ROS), which can damage cellular components such as lipids, proteins, and nucleic acids. To counteract this, plants have evolved complex metabolic pathways involving both enzymatic and non-enzymatic mechanisms to detoxify and process these harmful compounds. Oxidative stress in plants primarily arises from the overproduction of ROS, including hydrogen peroxide (H2O2), superoxide anions (O2•−), singlet oxygen (1O2), and hydroxyl radicals (•OH), by-products of metabolic activities such as photosynthesis and respiration. Exposure to xenobiotics significantly elevates ROS levels, causing cellular damage and disrupting metabolism. Plants employ a robust antioxidant defense system featuring enzymatic antioxidants like catalase (CAT), superoxide dismutase (SOD), and various peroxidases (POD). These enzymes collaborate to scavenge ROS, minimizing their detrimental effects. Additionally, plants produce numerous non-enzymatic antioxidants, such as flavonoids, phenolic acids, vitamins C and E, which neutralize ROS and regenerate other antioxidants, providing comprehensive protection against oxidative stress. Xenobiotic metabolism in plants involves three phases: Phase I (modification), Phase II (conjugation), and Phase III (compartmentalization), facilitated by enzymes like cytochrome P450 monooxygenases (CYPs), transferases such as glutathione S-transferases (GSTs), and ATP-binding cassette (ABC) transporters. Plant secondary metabolites, including alkaloids, terpenoids, and flavonoids, significantly contribute to detoxification and antioxidant defense. Understanding these oxidative processes and xenobiotic metabolism pathways in plants is crucial, as it offers insights into developing plant-based strategies to mitigate environmental pollution impacts and improve human health through enhanced detoxification and antioxidant mechanisms, as we discuss in this review.

With the acceleration of urbanisation and the increase in underground space use, how to provide a comfortable and healthy environment in underground space has become an important research topic. This study constructed an environmental decision-making model for underground space by integrating human perception evaluation and physical environment factors. The study analysed the influence of physical environment parameters on users' perceived experience through field data collection and questionnaire surveys. The data were in-depth analysed using single-indicator fitted regression analysis and XGBoost machine learning model. The results reveal the significant influence of physical parameters such as temperature, humidity, illuminance and wind speed on the comfort of users of underground spaces and determine the range of appropriateness of these physical environment parameters. The results provide a reliable theoretical basis for optimising the design and management of underground spaces and help to enhance the environmental quality and user experience of underground spaces.

In a time of ecological and social crises, designing new institutions is crucial for meeting future challenges. Institutions emerge from the imaginations of individuals, moving into social movements and crystallizing in legal structures. As present movements seek to develop tomorrow’s governances, they turn to decentralized structures: distributed networks with little hierarchy, characterized by a diversity of actors and dynamics. While movements and technologies may seek to utilize decentralized ideas, they lack design principles: only recently has it been possible to decentralize at the national stage. Designs may come from biology and ecological networks; fungi, plants, and slime molds in soil to the neurons, glia, and blood vessels within our own bodies. This article blends these biological analogies with present technologies like blockchain and Web3 to posit future decentralized institutions, movements and legalities that give greater consideration to the more-than-human world.

Low back pain (LBP) is a significant global health issue, contributing to considerable disability and socioeconomic burdens. The degeneration of the human intervertebral disc (IVD) is a critical factor in the pathogenesis of LBP. Recent studies have emphasized the significance of its specific set of genes and extracellular matrix (ECM) in IVD health. The Noggin gene has emerged as a critical factor due to its high expression levels in healthy nucleus pulposus cells (NPCs) observed in our previous research. In this study, we investigated the hypothesis that decreased Noggin expression in NPCs is associated with IVD degeneration, contributing to LBP development. A lentivirus-mediated RNAi was applied to knockdown Noggin expression in primary NPCs from six human donors. Cell viability analysis, XTT assay, and cell apoptosis were conducted to reveal the effect after transduction. After two weeks, colony formation unit (CFU) assay was used to examine the anchor-independent growth ability of transduced cells. Additionally, qPCR was performed to quantify cellular anabolism and catabolism makers at the transcript level. The results showed lentivirus-mediated downregulation of Noggin suppressed cell growth, cell viability, and colony formation, and induced apoptosis in human NPCs in vitro. Noteworthy, it hindered cellular anabolism and enhanced catabolism activity in human NPCs after transduction. Our findings indicated that the degeneration of human IVD is possibly related to decreased Noggin expression in NPCs. This research provides valuable insights into the role of Noggin in IVD homeostasis and its implications for LBP treatment.

This study evaluated the presence of Human Papillomavirus (HPV) and Bovine Leukemia Virus (BLV) DNA in breast cancer (BC) samples from women in Northeastern Brazil. We analyzed 186 formalin-fixed, paraffin-embedded tissue block samples using PCR and qPCR to detect HPV and BLV DNA. BLV DNA was not detected in any BC patients. However, HPV DNA was found in 46.2\% of the BC samples: 44.6\% were positive for HPV16, 3.5\% for HPV31, and 2\% were coinfected with HPV16 and HPV31. Additionally, we found an association between HPV-positive BC and Ki-67 expression (P=0.03, OR=1.85; CI=1.02-3.34), as well as between HPV31-positive BC and mitotic index (P=0.047). Although BLV was not detected in BC patients from Northeastern Brazil, the association between HPV and cell proliferation markers warrants further investigation, as HPV may contribute to the aggressiveness of BC in these patients.

The human eye is a vital source of collecting information worldwide via daily observation. The computer science area recognizes it in research as a human-computer interaction. Human blink is an integral part of this observation as a research topic. Several studies have been conducted on eye blinking and left-right eye movements. Previous studies conducted on the eye blink consider the availability of hardware devices high in the budget, high Precision, and low light expo-sure, unlike our study, which uses low-budget devices as simple as a webcam and more versatile usable techniques, incredibly both dim and high light to name a few. This study offers the observational approach via ResNet101v2, VGG-19, and Convolution Neural Network (CNN) architectures. Outperforming both VGG-19 and CNN in this study, ResNet101v2 achieves an impressive accuracy of 98.2%. In contemporary times, deep learning in AI is taking an advanced form. This study attempts to provide a new insight into the real-world implementation of the eye blink.

Arabinoxylans (AXs) are non-starch polysaccharides with complex structures naturally occurring in grains (i.e., barley, corn, and others) providing many health benefits, especially as prebiotics. AXs can be classified as water-extractable (WEAX) and water-unextractable (WUAX) based on their solubility, with properties influenced by grain sources and extraction methods. A large body of evidence shows that AXs exert an im-portant health impact, including glucose and lipid metabolism regulation and immune system enhancement, which is induced by the interactions between AXs and the gut microbiota. Recent research underscores the dependence of AX physiological effects on structure, advocating for a deeper understanding of struc-ture-activity relationships. While systematic studies on WEAX are prevalent, knowledge gaps persist re-garding WUAX, despite its higher grain abundance. Thus, this review reports recent data on WUAX struc-tural properties (chemical structure, branching, MW) in cereals under different treatments. It discusses WUAX applications in baking and its benefits deriving from gut fermentation.

Pedestrian trajectories are crucial for self-driving cars to plan their paths effectively. The sensors implanted in these self-driving vehicles, despite being state-of-the-art ones, often face inaccuracies in the perception of surrounding environments due to technical challenges for adverse weather conditions, interference from other vehicles sensors and electronic devices, and signal reception failure, leading to incompleteness in trajectory data. But for real-time decision making for autonomous driving, the trajectory imputation is no less crucial. Previous attempts to address this issue such as statistical inference and machine learning approaches have shown promise. Yet, the landscape of deep learning is rapidly evolving with new and more robust models emerging. In this research, we have proposed an encoder-decoder architecture, Human Trajectory Imputation Model, coined as HTIM, to tackle these challenges. This architecture aims to fill in the missing parts of pedestrian trajectories. The model has been evaluated using the Intersection drone inD dataset, containing trajectory data at suitable altitudes preserving naturalistic pedestrian behavior with varied dataset sizes. To assess the effectiveness of our model, we have utilized L1, MSE, Quantile and ADE loss. Our experiments have demonstrated that HTIM outperforms the majority of the state-of-the-art methods in this field, thus indicating its superior performance in imputing pedestrian trajectories.

Fire is a serious security threat that can lead to casualties, property damage, and environmental damage. However, despite the availability of object detection algorithms, challenges persist in detecting fires and smoke. These challenges include slow convergence speed, poor performance in detecting small targets, and high computational cost limiting deployments. In this paper, Fire smoke and human detection based on ConvNeXt and Mixed encoder (FCM-DETR), an end-to-end object detection algorithm based on Deformable DETR, is proposed. Firstly, we introduce ConvNeXt to take the place of Resnet, which greatly reduces the amount of computation and improves the ability to extract irregular flame features. Secondly, to effectively process multi-scale features, the original encoder is decoupled into two modules. Then Mixed encoder, an innovative encoder structure, is proposed, resulting in excellent performance of multi-scale fire and smoke features fusion. What can’t be overlooked is that the encoder block is compatible with any DETR-based models. Finally, the convergence speed is accelerated and the mean of average precision is improved by applying a novel loss function called Powerful IoU v2. The experimental results indicate that our model achieves the best detection accuracy compared to other models, with mAP reaching 66.7%, mAPs and Accuracyfire achieving impressive 50.2% and 98.05%, respectively.

Network centrality analyses have proven successful in identifying important nodes in diverse host-pathogen interactomes. The current study presents a comprehensive investigation of the human interactome and SARS-CoV-2 host targets. We first constructed a comprehensive human interactome by compiling experimentally validated protein-protein interactions (PPIs) from eight distinct sources. Additionally, we compiled a comprehensive list of 1,449 SARS-CoV-2 host proteins and analyzed their interactions within the human interactome, which identified enriched biological processes and pathways. Seven diverse topological features were employed to reveal the enrichment of SARS-CoV-2 targets in the human interactome, with Load centrality emerging as the most effective metric. Furthermore, a novel approach called CentralityCosDist was employed to predict SARS-CoV-2 targets, which proved effective in expanding the pool of predicted targets. Pathway enrichment analyses further elucidated the functional roles and potential mechanisms associated with predicted targets. Overall, this study provides valuable insights into the complex interplay between SARS-CoV-2 and the host cellular machinery, contributing to a deeper understanding of viral infection and immune response modulation.

Data4HumanRights training materials have been developed as open-source and tailored to limited resource settings where community data collectors often live and work. An overview of how the training material was developed together with community data collectors in Nigeria and Kenya is provided. The paper gives insights into the fundamental principles (i.e., inclusiveness, adaptive, limited resources, gender- and incentive-sensitive) and the structure of the open-access training materials. This resulted in 28 modules designed to be delivered in a face-to-face format in less than one day by a local trainer. To maximize adaptivity, the training modules can be mixed and matched (e.g., as individual modules or a learning path of several modules around a specific training need). The individual modules cover a range of methods and tools that are useful to human rights work, for example, documenting evictions, performing rapid needs assessments after acute crises, community profiling, and monitoring community development indicators. The training materials contain instructions for the facilitator(s) of the training and all necessary training materials to conduct the training. To maximize inclusivity, the training covers basic to advanced topics. Most training modules address basic training topics that can be optimally followed with a mobile phone (to avoid using a computer and printing handouts). The training was developed and implemented with local community groups in Nigeria and Kenya. The material is free to use, adapt, and translate into different contexts and languages.

Introduction: The trophoblast derived angiogenic factors are considered to play an important role in the pathophysiology of various complications of pregnancy. The aim of this study was to establish relationship between concentrations of soluble Human Leukocyte Antigen-G (sHLA-G) in maternal serum and amniotic fluid at 16-22 weeks of gestation and the sonographic measurements of the fetal and placental growth. Materials and methods: sHLA-G in serum and amniotic fluid, as well as fetal biometric data and placental volume and perfusion indices were determined in 41 uncomplicated singleton pregnancies. Results: The sHLA-G levels were unchanged both in amniotic fluid and in serum during mid-pregnancy. The sHLA-G level in serum correlated positively with amniotic sHLA-G level (β=0.63, p<0.01). Significant correlation was found between serum sHLA-G level and abdominal measurement (β=0.41, p<0.05) and estimated fetal weight (β=0.41, p<0.05). The amniotic sHLA-G level and placental perfusion (VI: β = -0.34, p<0.01 and VFI: β = -0.44, p<0.01, respectively) were negatively interrelated. A low amniotic sHLA-G level was significantly associated with nuchal translucency (r = -0.102, p<0.05). Conclusions: sHLA-G assayed in amniotic fluid might be a potential indicator for placental function, whereas the sHLA-G level in serum can be a prognostic modality for feto-placental insufficiency.

Ensuring access to water, sanitation, and hygiene (WASH) facilities, coupled with behavior change education in schools, is fundamental for fostering a conducive learning environment and promoting principles of inclusion, dignity, and equality. This study focuses on 12 primary schools in Anápolis, Brazil, encompassing 4,394 students and 248 teachers. WASH assessments were conducted through a questionnaire, observation of water and sanitation facilities and structures, and microbiological analysis. Over the period 2018 to 2020, the proportion of schools with safely managed drinking water surged from 42% to 92%. However, sanitation conditions in the schools were categorized as a basic level, with hygiene services rated as limited in 92% of the schools due to the absence of soap. While this study reveals that Anápolis schools generally align with WASH guidelines for basic/advanced drinking water services, there is a crucial need for enhancements in both hardware and software facilities to address sanitation and hygiene challenges.

In our increasingly urbanized world, precise and up-to-date maps of human settlements are essential for sustainable urban development policies. The availability of open-access Sentinel-2 data from the Copernicus program presents an opportunity to create a comprehensive global map of human settlements, offering a detailed view of built areas on a large scale. This study estimates large-scale built-up fractions using encoder-decoder deep learning architectures like U-net, Res-U-net, and Attention-U-net in the large and complex urban area of Delhi, India. Openly available datasets like Open Street Map (OSM) and Microsoft building footprint datasets are used to derive built-up fractions at 10×10m resolution cells for over 34,000 km2. Our results show that Attention-U-net with the Huber loss function performs the best in different built-up densities (i.e., urban, semi-urban or rural) with an R2 score of 0.631, while Res-U-net and U-net obtained an R2 score of 0.623 and 0.612, respectively. The investigated networks significantly improve the accuracy over the latest Global Human Settlement Layer product (GHSL-S2), which uses a deep-CNN and reaches an R2 of 0.387 in our case study area. The result of this study yields a valuable spatial layer for examining the spatial distribution of human settlements across the entire spectrum from rural to urban areas.

We propose Context-aware Feature Transformer Network (CaFTNet), a novel network for human pose estimation. To address the issue of limited modeling of global dependencies in convolutional neural networks, we design Transformerneck to strengthen the expressive power of features. Transformerneck directly substitutes the 3×3 convolution in bottleneck of HRNet with Contextual Transformer (CoT) block, while reducing the complexity of the network. Specifically, CoT first produces keys with static contextual information through 3×3 convolution. Then, relying on the query and contextualization keys, the dynamic contexts are generated through two concatenated 1×1 convolutions. Static and dynamic contexts are eventually fused as an output. Additionally, for the multi-scale networks, in order to further refine the features of the fusion output, we propose an Attention Feature Aggregation Module(AFAM). Technically, given an intermediate input, AFAM successively deduces attention maps along channel and spatial dimensions. Then, Adaptive refinement module(ARM) is exploited to activate the obtained attention maps. Finally, the input undergoes adaptive feature refinement through multiplication with the activated attention maps. Through the above studies, our lightweight network provides a powerful clue for detection of keypoints. Experiments are implemented on the COCO and MPII datasets. The model achieves 76.2 AP on the COCO val2017. Compared to other methods with the CNN as the backbone, CaFTNet reduces the number of parameters by 72.9 %. On the MPII, our method uses only 60.7% of the number of parameters, acquiring semblable results to other methods with the CNN as the backbone.

Abstract: Antimicrobial peptides (AMPs) are short peptides, usually made up of less than 100 amino acid residues. They are found in many living organisms and are an important factor in their innate immune systems. AMPs can be extracted from various living sources including bacteria, plants, animals and even humans. They are usually cationic peptides with an amphiphilic structure, which allows them to easily bind and interact with the cellular membranes of viruses, bacteria, fungi and other pathogens. They can act against both Gram-negative and Gram-positive pathogens and have various modes of action against them. Some attack the pathogens membrane, while others target their intracellular organelles as well as their nucleic acids, proteins and metabolic pathways. A crucial area of use of AMPs is in their ability to help with emerging antibiotic resistance: some AMPs are active against resistant strains and are susceptible to peptide engineering. This review considers AMPs from three key sources: plants, animals and humans, as well as their modes of action and some AMPs sequences.

This study investigated the capability of vocal-identity-cloning Artificial Intelligence (AI) to encode human-specific confident, doubtful, and neutral-intending emotive states. Linear mixed-effects models and machine learning classification with eXtreme Gradient Boosting were employed to examine the underlying acoustic signatures from 2,700 audio clips, comprising of sentences spoken by human speakers and two sets of equivalences (AI-Geography/AI-Trivia, based on the trained text) generated by voice-cloning models designed to clone human speakers’ identities. Compared with neutral-intending voice, human speakers lengthened their vocal tract, raised the fundamental frequency, and increased Chroma constant-Q transform when they intended to be confident; An opposite pattern was shown when they intended to be doubtful. The two sets of AI sounds displayed a similar pattern to human speech, suggesting a shared mechanism for encoding vocal expression across sources. The 1,000 times training-testing classification models reported an in-group advantage for AI sources. The algorithms, trained on AI-Geography/AI-Trivia, resulted in higher accuracies when tested within these AI sources than when tested on human audio. All between-source classifications reported above-chance-level (1/3) accuracies. These findings highlighted that voice-cloning AI, the widely used conversational agent, can learn and generate human-specific vocally-expressed confidence.

Advances in biomimetic triboelectric nanogenerators (TENGs) have significant implications for electronic skin (e-skin) and human-machine interaction (HMI). Emphasizing the need to mimic complex functionalities of natural systems, particularly human skin, TENGs leverage triboelectricity and electrostatic induction to bridge the gap in traditional electronic devices' responsiveness and adaptability. The exploration begins with an overview of TENGs' operational principles and modes, transitioning into structural and material biomimicry inspired by plant and animal models, proteins, fibers, and hydrogels. Key applications in tactile sensing, motion sensing, and intelligent control within e-skins and HMI systems are highlighted, showcasing TENGs' potential in revolutionizing wearable technologies and robotic systems. This review also addresses the challenges in performance enhancement, scalability, and system integration of TENGs. It points to future research directions, including optimizing energy conversion efficiency, discovering new materials, and employing micro-nanostructuring techniques for enhanced triboelectric charges and energy conversion. The scalability and cost-effectiveness of TENG production, pivotal for mainstream application, are discussed along with the need for versatile integration with various electronic systems. The review underlines the significance of making bioinspired TENGs more accessible and applicable in everyday technology, focusing on compatibility, user comfort, and durability. Conclusively, it underscores the role of bioinspired TENGs in advancing wearable technology and interactive systems, indicating a bright future for these innovations in practical applications.

The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, what is important for regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses O2 and CO2 are well known; conversely, the mechanisms by which it senses pH variations are almost unknown. Here we used immunohistochemistry to investigate how the human carotid body contributes to the detection acidosis analyzing whether it expresses acid-sensing ion channels (ASIC) ion channels, and to know if these channels are in the cells chemosensory glomic cells or in the afferent nerves. ASIC1, ASIC2 and ASIC3, and to a much lesser extent ASIC4, immunoreactivity was detected in subpopulations of type I glomus cells, as well as in the nerves of the carotid body. In addition, immunoreactivity was found for all ASIC subunits in neurons of the petrosal and superior cervical sympathetic ganglia, where afferent and efferent neurons are located, respectively, innervating the carotid body. This study reports for the fist time the occurrence of ASIC proteins in the human carotid body, demonstrating that they are present in glomus chemosensory cells (ASIC1 &lt; ASIC2 &gt; ASIC3 &gt; ASIC4) and nerves, presumably both afferent an efferent supplying the organ. These results suggest that the detection of acidosis by the carotid body can be mediated by ASIC ion channels present in the type I glomus cells or directly by sensory nerve fibers.

Micro-plastics (MPs) become significantly important to society since their commercial manufacture began in the 1950s because of their low production costs, stability, light weight, and adaptability. Plastic was produced in 400 million tons worldwide in 2020, compared to 1.5 million tons just 60 years earlier. Scientists predict that by 2050, plastic production would increase to 33 billion tons. MPs are a rising scientific and societal issue in the environment as a new type of contaminant that is widely disseminated in aquatic ecosystems and organisms. Aquatic species that come into contact with MPs for an extended period of time experience ecotoxicity, which includes oxidative stress, genotoxicity, neurotoxicity, and reproductive issues. In aquatic environments, MPs not only transport chemicals within animals but also serve as mediators for chemicals or other contaminants. As a result of co-exposure of MPs and chemical contaminants, various toxicity incidences involving aquatic organisms have been reported. The final exposure of MPs occurs in humans through biomagnification of aquatic edible products. This study presents an important overview of the literature on MP ingestion by aquatic biota. It covers topics such as the present scenario of plastic pollution worldwide, the pathways that MPs take to reach humans and aquatic species, the eco-toxicological impacts of MPs on aquatic species, the relation between MPs and other environmental pollution and the ensuing effects on aquatic organisms, and the MP pollution status in Bangladesh.

3D human pose estimation has made significant advancements through the integration of deep learning techniques. This survey provides a comprehensive review of recent 3D human pose estimation methods, with a focus on monocular images, videos, and multi-view cameras. Our approach stands out through a systematic literature review methodology, ensuring an up-to-date and meticulous overview. Unlike many existing surveys that categorize approaches based on learning paradigms, our survey offers a fresh perspective, delving deeper into the subject. For image-based approaches, we not only follow existing categorizations but also introduce and compare significant 2D models. Additionally, we provide a comparative analysis of these methods, enhancing the understanding of image-based pose estimation techniques. In the realm of video-based approaches, we categorize them based on the types of models used to capture inter-frame information. Furthermore, in the context of multi-person pose estimation, our survey uniquely differentiates between approaches focusing on relative poses and those addressing absolute poses. Our survey aims to serve as a pivotal resource for researchers, highlighting state-of-the-art deep learning strategies and identifying promising directions for future exploration in 3D human pose estimation.

This research investigates the utilization of human driving models in autonomous vehicles, particularly in scenarios with minimal or no interactions with other vehicles. Human driving models provide valuable insights into driver behavior and play a crucial role in shaping the behavior of autonomous vehicles, enhancing their performance and user experience. The primary focus of the study is the creation of a planning model for autonomous vehicles when navigating roundabouts in the absence of traffic. This model seeks to emulate human driving behavior, ensuring predictability, safety, optimization of traffic flow, and adaptation to various roundabout geometries. To achieve this, the research introduces a trajectory model that takes into account geometric attributes and speed variations within roundabouts. The model is calibrated using empirical data and generalizes parameters through statistical regression methodologies. This model is referred to as "MRoundabout" and is evaluated for its consistency in generating plans that closely mimic human driving behavior within roundabouts. While the study presents a promising approach, it acknowledges limitations related to the model's reliance on geometric attributes and its inability to account for external factors like weather conditions. This research underscores the importance of bridging the gap between theoretical research and practical application, with the aim of enhancing safety and the overall user experience in real-world driving scenarios.

Molnupiravir, a prodrug known for its broad antiviral activity, demonstrated efficacy in animal models of Covid-19, prompting clinical trials where initial results indicated a significant effect against the disease. However, subsequent clinical studies did not confirm these findings, leading to the rejection of molnupiravir for permanent market authorization in many countries. This report critically assessed 19 studies published in 17 reports that investigated the efficacy of molnupiravir in animal models of Covid-19 with the purpose of determining how well the design of these models informed human studies. We found that the administered doses of molnupiravir in most studies involving animal Covid-19 models were disproportionately higher than the dose rec-ommended for human use. Specifically, when adjusted for body surface area, half of the doses of molnupiravir used in the animal studies were more than twice as high as the human dose. Addi-tionally, the drug was frequently given prophylactically or shortly after SARS-CoV-2 inoculation in these models, in contrast to clinical trials where such timing is not consistently achieved. Furthermore, the recommended five-day treatment duration for humans was exceeded in several animal studies. Collectively, we suggest that these design elements in the reported animal studies contributed to a bias favoring molnupiravir, and thus inflated expectations for its efficacy against Covid-19. Addressing these elements may offer avenues to enhance the clinical efficacy of mol-nupiravir for treatment of Covid-19 that include dose increment, early treatment, and admin-istration by inhalation along with use of molnupiravir in antiviral combination therapy.

Background. Human Milk (HM) is a dynamic nourishment: its composition is influenced by several conditions such as gestational age, maternal diet and ethnicity. It appears important evaluate the impact that gestational diseases have on HM components and if their presence, as source of oxidative stress in the mother, influence milk’s redox homeostasis. To assess Preeclampsia (PE) and Gestational Diabetes Mellitus (GDM) effect on milk’s total oxidative load, we chose to investigate both oxidative and antioxidant aspects, with respectively Lipid hydrop-eroxides (LOOHs) and Glutathione (GSH)). Methods. GSH and LOOHs were quantified using an ELISA test. To investigate the effect of pathology on redox homeostasis, mixed linear model with unistructural covariance structure was performed. Results. A total of 120 mothers were re-cruited. The GSH concentration results significant lower in GDM women than in healthy only in colostrum (p<0.01). No other differences emerged. LOOHs was not detectable in almost all the samples. Discussion. Our study is the first to extensively evaluate these components in the HM of women with these gestational pathologies. The main observation is that GDM can alter the anti-oxidant composition of HM mainly in colostrum, but in case of PE, the composition of HM milk is preserved.

Per- and polyfluoroalkyl substances (PFASs) are a group of fluorinated, organic, man-made chemicals; they do not occur naturally in the environment. This study aimed to determine the profile and content of PFASs in volunteers’ blood plasma and urine after fermented red beetroot juice consumption and correlated it with the blood parameters. During 42 days, 24 healthy volunteers intake 200 mL/60 kg of body weight fermented red beetroot juice. PFASs were analyzed using micro-HPLC-MS/MS method. Five perfluoroalkyl substances were found in human body fluids. After juice consumption, PFASs, due to the consumption of food contaminated with them, was discovered that for perfluorocarboxylic acids, a downward trend was observed, while for perfluoroalkane sulfonates, their plasma content showed a statistically significant upward trend. Analysis of the hematology parameters indicated that the intake the fermented red beetroot juice showed a significant decrease in mean corpuscular volume (MCV), platelets concentration, mean platelet volume (MPV), platelet large cell ratio (P-LCR) at the significance level p < 0.01, and he-matocrit (p < 0.05). On the other hand, the dietary intervention also indicated a significant (p < 0.01) increase corpuscular/ cellular hemoglobin concentration (MCHC). In the case of blood biochemi-stry, no significant change in blood samples after intake the fermented beetroot juice was observed. However, a decreasing tendency of total cholesterol and low-density lipoprotein concentration (LDL-C) were observed. Based on the presented results, there is a need to analyze and monitor health-promoting food regarding undesirable substances and their impact on consumer health.

Solar energy is a renewable and sustainable source of energy, and it has been used in addition of specified technologies, such as solar cooling. The demand for cooling continues to increase in line with environmental changes and a greater desire for human comfort. This study primarily aimed to analyze the performance of an evaporative cooling system powered by solar energy and assess the economic and environmental impact of this system. In this system, hot, dry incoming air is used to evaporate water, thus transforming part of its sensitive heat into latent heat without any variation in enthalpy or total heat. The outputted air then has a lower temperature and a greater humidity, thus providing a more comfortable, healthier environment for its users. To achieve this study’s objectives, numerical code was developed and implemented in MATLAB. The obtained results reveal that the efficiency of the evaporative cooler exceeds 90%, with maximum efficiency being reached at a high wet-bulb depression, while minimum efficiency was observed when the dry air has a high relative humidity and a low dry-bulb temperature.

In recent years, with the rapid development of computer vision technology and the popularization of intelligent hardware, as well as people’s increasing demand for intelligent products for human-computer interaction, visual grounding technology can help machines and humans identify and locate objects, thereby promoting human-computer interaction and intelligent manufacturing. At the same time, human-computer interaction is constantly evolving and improving, becoming increasingly intelligent, humane, and efficient. This paper proposes a new VG model and designs a language verification module that uses language information as the main information to increase the model’s interactivity. Additionally, we propose the combination of visual grounding and human-computer interaction, aiming to explore the research status and development trends of visual grounding and human-computer interaction technology, as well as their application in practical scenarios and optimization directions, to provide references and guidance for relevant researchers and promote the development and application of visual grounding and human-computer interaction technology.

Accurately estimating a person's age is crucial for identity verification at all critical checkpoints, including airports, land borders, and seaports. Human gait may be used as a biometric identifier and indicator of age, among other things. This study aims to develop methods for estimating a person's age by observing their walk. In this paper, a novel technique for preprocessing the proposed gait dataset has been used by utilizing a combination of the gait energy image (GEI), the accumulated frame difference energy image (AFDEI), and the invariant moment of the image. The proposed technique outperformed state-of-the-art methods in terms of accuracy. The proposed method was tested and evaluated using a convolutional neural network (CNN), and it achieved an average accuracy of 90.35% across 14 different view angles within 5 K-Fold, the proposed method resulted in 94.68% and 94.54% in 30º and 75º view degree respectively. concluding that the approach is effective and promising for estimating age using human gait.

The purpose of the research was to examine and assess the relation between the pilot’s concentration and reaction time with specific brain activity during short-haul flights. In order to obtain this task, participants took part in 1-hour long flight sessions performed on FNPT II class flight simulator. During the flight auto–pilot was activated and subjects were instructed to give response to unexpected events that were occurring during the flight. Brainwaves of each participant were recorded with Emotiv EPOC+ Scientific Contextual EEG device. Statistical analyses of the results were presented in the article.

Chlamydia trachomatis (CT) is an obligate intracellular bacterial pathogen known to cause trachoma, sexually transmitted infections, and reactive arthritis. The more serious sequelae of these diseases, blindness from trachoma and pelvic inflammatory disease from chlamydial STD are immunopathological responses to chronic infections. With the abundance of dendritic cells in CT infection sites and their central importance in immune regulation, the susceptibility of dendritic cells to CT infection was investigated in vitro. CT (serovar F) was grown and extracted from HeLa 229 cells for infection of human dendritic cells (DCs). Human DCs were generated from peripheral blood mononuclear cells in the presence of GM-CSF and IL-4. Infected DCs were harvested at 0, 4, 24, and 48 h after infection and immunostained. Results from fluorescence microscopy showed that DC can be infected and can support the growth of CT in vitro. Intracellular elementary and reticulate bodies of CT were detected, and reticulate bodies were shown to multiply over the 48 h-cycle in DC. At 48 h, intracellular elementary bodies were detected in some DCs. These results may provide new insights into the possible participation of DC in sustaining chronic or latent infection, or even dissemination of CT infection to other sites.

Background and aim: Human Papillomavirus (HPV) is sexually transmitted, one of the three most common sexually transmitted infections (STIs) in both males and females, and the most common viral STI. A crucial public health strategy to protect people against HPV is through vaccination, which has shown its effectiveness in preventing HPV related diseases. Presently three types of vaccines are available (bivalent, quadrivalent, and nonavalent) and they all target at least the two most oncogenic virus genotypes (HPV 16, 18). In the past few years, the need to implement vaccination programmes that include all genders has been discussed in order to achieve herd immunity against HPV. To date, only a few countries have included young males in their vaccination programmes, so we aim through this review to provide an overview of the epidemiology of HPV and prevention strategies to prevent it, reporting the latest findings from the scientific literature.

Cyber-physical security is vital for protecting key computing infrastructure against cyber attacks. Individuals, corporations, and society can all suffer considerable digital asset losses due to cyber attacks, including Data loss, theft, financial loss, reputation harm, company interruption, infrastructure damage, ransomware attacks, and espionage. A cyber-physical attack harms both digital and physical assets. Cyber-physical system security is more challenging than software-level cyber security because it requires physical inspection and monitoring. This paper proposes an innovative and effective algorithm to strengthen Cyber-Physical Security (CPS) with minimal human intervention. It is a Human Activity Recognition (HAR)-based approach where a GoogleNet-BiLSTM network hybridization has been used to recognize suspicious activities in cyber-physical infrastructure perimeter. The proposed HAR-CPS algorithm classifies suspicious activities from real-time video surveillance with an average accuracy of 73.15%. It incorporates Machine Vision at the IoT Edge (Mez) technology to make the system latency tolerant. Dual-layer security has been ensured by operating the proposed algorithm and GoogleNet-BiLSTM hybrid network from a cloud server, which ensures the security of the proposed security system. The innovative optimization scheme makes it possible to strengthen cyber-physical security with $4.29 per month only.

Viruses can induce the fusion of infected and neighboring cells, leading to the formation of syncytia. Cell-cell fusion is mediated by viral fusion proteins on the plasma membrane of infected cells that interact with cellular receptors on neighboring cells. Viruses use this mechanism to spread rapidly to adjacent cells or escape host immunity. For some viruses, syncytium formation is a hallmark of infection and a known pathogenicity factor. For others, the role of syncytium formation in viral dissemination and pathogenicity remains poorly understood. Human cytomegalovirus (HCMV) is an important cause of morbidity and mortality in transplant patients and the leading cause of congenital infections. Clinical HCMV isolates have broad cell tropism but differ in their ability to induce cell-cell fusions, and little is known about the molecular determinants. We developed a system to analyze HCMV glycoprotein B (gB) variants in a defined genetic background. HCMV strains TB40/E and TR were used as vectors to compare the fusogenicity of six gB variants from congenitally infected fetuses with those from three laboratory strains. Five of them conferred the ability to induce fusion of MRC-5 human embryonic lung fibroblasts to one or both backbone strains, as determined by a split GFP-luciferase reporter system. The same gB variants were not sufficient to induce syncytia in infected ARPE-19 epithelial cells, suggesting that additional factors are involved. The system described here allows a systematic comparison of the fusogenicity of viral envelope glycoproteins and may help to clarify whether fusion-promoting variants are associated with increased pathogenicity.

Human and animal waste, including waste products originating from human or animal digestive systems such as urine, feces, and animal manure, have constituted a nuisance to the environment. Inappropriate disposal and poor sanitation of human and animal waste often cause negative impacts on human health through contamination of the terrestrial environment, soil, and water bodies. Therefore, it is necessary to convert these wastes into useful resources to mitigate their adverse environmental effect. The present study provides an overview and research progress of different thermochemical and biological conversion pathways for the transformation of human- and animal-derived waste into valuable resources. The physicochemical properties of human and animal waste are meticulously discussed as well as nutrient recovery strategies. In addition, a bibliometric analysis is provided to identify the trends in research and knowledge gaps. The results reveal that the U.S.A, China and England are the dominant countries in the research areas related to resource recovery from human or animal waste. In addition, researchers from the University of Illinois, the University of California Davis, the Chinese Academy of Science and Zhejiang University are front runners in research related to these areas. Future research should be centred on developing technologies for the on-site recovery of resources, exploring integrated resource recovery pathways, and exploring different safe waste processing methods.

Human motor planning combines such interesting aspects as modular organization in motor primitives and characteristics of stochastic optimality. Motor primitives aided in the description of motor skill learning. In this study, for the first time, an interpretation is proposed to explain how motor primitives are shaped in a stochastic optimality process.To this end, sensory information is assumed to be random variables at each moment. Theoretically, the results confirmed that the variability of a movement increased with passing time. As a result, motor planning should be performed in shorter durations to achieve an accurate movement. It means that a task should be divided into several subtasks at various stages of time. Moreover, theoretically, the results confirmed that motor planning is statistically optimal if the movement is planned in minimum jerk space, which is in accordance with what is reported in the literature. Comparing the proposed mathematical model with the results of experimental data in two different types of motor action (i.e., arm reaching movement and sit-to-stand transfer from a chair) confirmed that the proposed model can be considered a framework to investigate the effect of bottom-up information on human motor planning level. Further experiments need to investigate it more deeply.

Residents’ perceptions on ecosystem services (ESs) provided by urban green spaces (UGSs) are vital for developing sustainable cities. However, there has been limited research on this issue in Malaysia. The aim of this paper is to assess Malaysians’ perceptions on ESs provided UGSs and how their perceptions are influenced by different socio-demographic factors. We used a structured online questionnaire and obtained 645 responses on 16 ESs from regulatory, cultural, and supporting ES categories. Descriptive statistics and binary logistic regressions were used for data analysis. In general, city dwellers have high positive perceptions on ESs provided by UGSs. Their perceptions for global climate regulation, noise buffering, pest regulation, spiritual, and research and education services would likely to significantly increase as they grow older. Households with moderate income have significantly greater perceptions of microclimate regulation, air filtration and aesthetic services. Having non-tertiary level education would significantly lower their perceptions of global climate and microclimate regulation, spiritual, aesthetics, and recreational services. Tertiary educated residents with non-environmental degrees also showed similar associations. Infrequent visits to UGSs would likely to significantly reduce the perception of global climate regulation, noise buffering, runoff reduction, erosion control, spiritual, and aesthetics services. People living far (>5km) from the UGSs also have significantly lower perceptions of microclimate regulation, air filtration services. Suggestions are made to incorporate ESs related content in pre-university curriculum and establishing small parks in neighborhoods to improve knowledge and uses of UGSs. These findings would assist urban planners designing environmentally sound urban policies and thereby improving human wellbeing in the cities.

The poor success rate of preclinical drugs which target the nervous system is related to the highly complex nature of brain physiology and pathophysiology. For in vitro drug screening in this field, the two-dimensional (2D) approach - where cells are incubated in a monolayer - is not physiologically relevant. In contrast, in vivo rodent models are very low throughput and expensive. As such, improved, well-characterised three dimensional (3D) in vitro models should be employed to bridge the gap between 2D in vitro primary screening and rodent models by incorporating aspects of the in vivo brain environment. These key features include the extracellular matrix (ECM) and a multidimensional relationship of supporting cells. In this study, a neural progenitor cell line was differentiated into the main types of cells found in brain, including neurons and astrocytes. This model was designed in a 3D extracellular matrix replacement in a minimalised manner. Different culture formats using multi-well plates were explored and a high-throughput cellular imaging platform was applied. Next, we applied this model to assess neural toxicity of a newly synthesised dual-specificity tyrosine-regulated kinase 1 (DYRK1) inhibitor derived from natural compounds, which is structurally related to a natural DYRK1A inhibitor harmine, K04179. Chemical studies have already shown that K04179 has higher specificity to DYRK1A compared with previously reported DYRK1 inhibitors, such as INDY. We have produced the first report of the biological effects of this new compound on the new 3D minimalised mid-brain model with quantification of both neuronal and astrocyte populations in tandem, revealing differential toxic effects of DYRK1 inhibitor compounds K04179 and INDY on neurons and glia.

COVID-19 is the most severe health crisis of the 21st century. COVID-19 presents a threat to almost all countries world-wide. The restriction of human mobility is one of the strategies used to control the transmission of COVID-19. However, it has yet to be determined how effective this restriction is in controlling the rise in COVID-19 cases, particularly in major capital cities such as Jakarta, Indonesia. Using Facebook's mobility data, our study explores the impact of restricting human mobility on COVID-19 case control in Jakarta. Our main contribution is showing how the restriction of human mobility data can give important information about how COVID-19 spreads in different places. We proposed modifying a global regression model into a local regression model by accounting for the spatial and temporal interdependence of COVID-19 transmission across space and time. We applied Bayesian hierarchical Poisson spatiotemporal models with spatially varying regression coefficients. We estimated the regression parameters using an Integrated Nested Laplace Approximation. We found that the local regression model with spatially varying regression coefficients outperforms the global regression model based on DIC, WAIC, MPL, and R2 criteria for model selection. In Jakarta's 44 districts, the impact of human mobility varies significantly. The impacts of human mobility on the log relative risk of COVID-19 range from –4.445 to 2.353. The prevention strategy involving the restriction of human mobility may be beneficial in some districts but ineffective in others. Therefore, a cost-effective strategy had to be adopted.

In the context of the pandemic caused by Covid-19, an important aspect that was affected and changed was the way people communicate. Communication efficiency can be improved by using modern technologies, for example: mobile devices, digital audio and video platforms, text platforms, social media and more. For further economic success, digital skills are needed not only for executives, but also for managers, in order to be able to lead teams and avoid situations of stress and tension in the virtual environment. This scientific paper includes a primary research of how the pandemic affected the communication at the workplace. For this a qualitative analysis was first performed, in which the interview was used as a working tool. Next, on the basis of a questionnaire, a hypothesis was formulated and validated afterwards through multiple linear regression. This research highlighted the particularly important role of communication and, in this context, the role of the remote team leader. In order to keep the team motivated and interested in the virtual environment, the manager must use modern tools to motivate, support and express his appreciation when the team, respectively the members of his team, achieve their goals successfully.

Background: With new challenges to the health system, many new competencies within the scope of teaching public health need to be addressed in medical schools’ curricula such as disaster risk management and health system science. The aims of this study were to identify the needs of public health competencies for medical doctors in Thailand and to assess the level of integration for technical collaboration in teaching public health. Method: A total of 17 out of 21 Thai medical schools participated in the national survey. Qualitative inquiries applied focus group interviews of community representatives from ten sample villages and in-depth interviews of representatives from stakeholder organizations particularly employers. The list of public health competencies framework recommended by WHO-SEARO was applied. Quantitative analysis applied descriptive analysis using STATA 15 and qualitative findings were validated by interrelating the meaning of themes from Word Clouds created in NVivo12. Data integration applied a mixed-method Quan-qual approach. Results: 17 medical schools returned the questionnaires (80.95 % yield). The most common regionally-defined public health competencies (in over 70% of schools) were shown to be: Biostatistics, Community Medicine, Epidemiology, Family Medicine, Medical Ethics and Professional Laws, Preventive Medicine, Health Promotion, Holistic Care, and Research. The curriculum in only one medical school lacked Health Economics, whilst Disaster Management was lacking in two other schools. Discipline-based subjects were found to be more prevalent than interdisciplinary competencies. A variety of methods were being applied for teaching public health. The majority of the schools applied lecture as the main teaching method and multiple-choice questions as the main assessment method. Thai communities expect the doctors to get in touch with the community more often, lead the primary health care team through training the health professionals and community health volunteers, and educate the community for better health. Conclusion: Human resource is the main challenge in addressing interdisciplinary competencies. It is necessary to establish a collaborating mechanism among the big and small medical schools and the faculties of public health to improve the teaching of public health to undergraduate students in medical schools. There is also a need to strengthen the health system science and leadership so that future MDs can lead health service delivery according to the needs of their employers such as the Ministry of Public Health and the Rural Doctors Association. The findings of this study may help to identify a national framework of public health core competencies for medical schools and create a common platform for interdisciplinary collaborations.

Purpose: The use of smart devices has increased greatly in the last ten years with users reaching out to the possibility to do more with them especially in the networking front. In this context there is a need to understand the connection between users’ social demographic factors and their way to related to their smart devices. Objective: This study was designed to evaluate the senso of belonging of a community in order to evaluate intangible benefits that employees may gain from a more immerse relationship with their devices. Method: We used a dataset of 586 anonymous respondent of an existing survey designed for capturing the relationships that humans develop with their smart devices. In particular, we investigate the relationships with smart device and particular background variables of the respondents using a chi-square test. Results: The study showed that there is a significant relationship between users’ sex and smart device type and their dependency on smart device. Male tends to think that smart device (in general) enables them to connect with a larger community. At the same time, female using smart phones feels more connected more to large community than when using other smart devices. Conclusion: This study provided several significant findings that confirm and strength previous literature works on the subject. In addition, socio demographics variables (like gender) as well as the type of smart device present a correlation between the smart device users and their tendency to stay in touch with a larger community.

The reduction of biodiversity loss is one of the targets of the 2030 Agenda for Sustainable Development. The protection of endangered species is critical for conserving global biodiversity. Asian elephants，as one of the last few mega-herbivores on Earth, are currently threatened by climate changes and anthropogenic modifications. The modelling of their living habitats is of top priority to the conservation of Asian elephant. In this study, we used the maximum entropy model (MaxEnt) to identify the current and potential future habitats of Asian elephants in South and Southeast Asia. We performed analyses for future projections with 17 scenarios by using the present results as baseline. To optimise the modelling results, we delineated the core habitats by using the Core Mapper Tool and compared them with existing protected areas (PAs) through gap analysis. The results showed that the current total area of core habitats is 491,455 km2 in size and will be reduced to 332,544 km2 by 2090 under SSP585 (the shared socioeconomic pathway). The projection analysis under differential scenarios suggested that most of the core habitats in the current protected areas would remain stable and suitable for elephants in the future. However, the remaining 75.17% of the core habitats lay outside the current PAs, and finally we mapped approximately 219,545 km2 of suitable habitats as priority protected areas in the future. Although our model did not perform well in some regions, our analyses and findings still could provide useful references to the planning of protected areas and conservation of Asian elephant.

The impact of nutrients on the environment, particularly on water bodies, has led to extensive studies for nutrient control. Within this context, studies have been focused on source separation of human urine from domestic wastewater to recover nutrients. Potassium is one of the most important components of human urine. However, data on potassium removal or recovery are quite limited except for some indirect information through use of zeolites for mostly ammonia removal. Potassium struvite or K-struvite (MgKPO₄·6H₂O) is a sparingly soluble salt belonging to struvite and has the potential of being used as a means of potassium and phosphate recovery from segregated human urine. This study aimed to assess the potential of K-struvite precipitation for control and recovery of nutrients. Within this context, K-struvite precipitation experiments were performed on both synthetically prepared samples and synthetic human urine solution to determine effect of operation parameters i.e. pH, stoichiometry, and temperature on potassium recovery performance. Results indicated that process performance as well as type of solid phases co-precipitated with K-struvite were closely related to initial potassium concentration, pH and reaction stoichiometry. At pH 10, the potassium recovery efficiency was maximized up to 87% by application of 100% excess dose of Mg and P for both synthetic samples and synthetic human urine solution. On the other hand, application of excess dose of K did not provide any improvement in K recovery efficiency. The effect of temperature on solubility of K-struvite was insignificant at the temperature of 24-90°C. Solid phase analyses confirmed that K-struvite was co-precipitated with either Mg₃(PO₄)₂, MgNaPO₄·7H₂O, or MgHPO₄·7H₂O depending on pH and stoichiometry instead of a pure compound.

This paper attempts to compare two different approaches to solve the problem of accident rates prediction based on human factors for industrial workers. One of the methods has already been done using Fuzzy c-Means Clustering and proved to be working with decent results. The second method which will be covered in this paper is using Artificial Neural Networks. The primary goal of this work is to insure that ANN will work efficiently in such prediction problem. The second goal is to reveal the fact that which one of the two selected methodologies is better at defining the estimation of accident rates among people who work in different industrial fields. The purpose has been achieved when the outcome of the ANN was obtained and compared accordingly with the output of the research previously carried out with Fuzzy c-means clustering method. Comparing the outcomes of these two different methods gave an immense insight on which features are more important than others when it comes to laborers properties with completely different background such as varying levels of health, knowledge, experience, training and physical properties. At the end of the research, it becomes clear that accident rates estimation for laborers with properly trained Artificial Neural Network gives better results when it is compared with Fuzzy c-Means Clustering method. Standard deviation method was used to calculate the validity of ANN technique. The result was compared with Fuzzy c-mean clustering technique. Impressive improvement of 8.8% in the accident rate prediction was achieved using Tailored-Made-ANN.

Aiming the characterization of road dusts on the surrounding villages of an active mine and to assess metal(loids) bioaccessible fractions, a dust samples survey was undertaken. To achieve these objectives, pseudototal element concentration, mineralogical composition, Enrichment factor and in vitro extraction UBM assay for both gastric (G) and gastrointestinal (GI) phases, were determined. Results obtained revealed that arsenic represents the higher risk to humans, with mean pseudototal values higher than the maximum reference value range. Enrichment factor points to As significant to very high enrichment in all villages. Particles enriched in As, Ca, Fe, Cu, Al, Ti were identified by SEM-EDS in weathered agglomerates, linked to mine wastes and long-distance transport, both by wind and/or traffic. Arsenic bioaccessibility fraction (%BAF) presents low values in the studied samples, possibly due to low Fe complexes solubility with adsorbed As, limiting arsenic release and reducing its bioaccessibility. Concentrations of Cd bioaccessible G and GI phases are within the reference values range, while for Cu are above and for Pb are lower, than reference value range. Results show that the pseudototal fraction risk is overestimated when comparing to BAF (bioaccessible fraction), nevertheless, total G and GI risk are above the carcinogenic target risk (1.00E-06) in most samples. Carcinogenic risk of the bioaccessible contaminants shows that As is the element that represents higher risk for developing cancer over a lifetime, being ingestion the main risk route.

The recent SARS-CoV-2 pandemic, which is causing COVID 19 disease, has taught us unexpected lessons about the dangers of human extinction through highly contagious and lethal diseases. As the COVID 19 pandemic is now being controlled by various isolation measures, therapeutics and vaccines, it became clear that our current lifestyle and societal functions may not be sustainable in the long term. We now have to start thinking and planning on how to face the next dangerous pandemic, not just overcoming the one that is upon us now. Is there any evidence that even worse pandemics could strike us in the near future and threaten the existence of the human race? The answer is unequivocally yes. It is not necessary to get infected by viruses of bats, pangolins and other exotic animals that live in remote forests in order to be in danger. Creditable scientific evidence indicates that the human gut microbiota harbor billions of viruses which are capable of affecting the function of vital human organs such as the immune system, lung, brain, liver, kidney, heart etc. It is possible that the development of pathogenic variants in the gut can lead to contagious viruses which can cause pandemics, leading to destruction of vital organs, causing death or various debilitating diseases such as blindness, respiratory, liver, heart and kidney failures. These diseases could result n the complete shutdown of our civilization and probably the extinction of human race. In this essay, I will first provide a few independent pieces of scientific facts and then combine this information to come up with some (but certainly not all) hypothetical scenarios that could cause human race misery, even extinction. I hope that these scary scenarios will trigger preventative measures that could reverse or delay the projected adverse outcomes.

Small and medium-sized family farms are the place of life and source of income for about half of the population. The aim of the analysis was to determine the relationship between Eco-Efficiency, Human Capital Efficiency in small and medium-sized family farms. The analyses were carried out using an economic measure (value of agricultural production per work hour calculated per 1 ha) and synthetic measures: human capital and environmental measures. The synthetic measures were determined using the CRITIC-TOPSIS method by defining weights for variables used in the synthetic measures. The analyses covered five countries, namely: Lithuania (960 farms), Moldova (532 farms), Poland (696 farms), Romania (872 farms), and Serbia (524 farms). All the countries qualified for analysis are characterised by a high fragmentation of agricultural holdings. The analyses carried out allowed us to formulate the following conclusions: the Eco-Efficiency and Human Capital Efficiency indexes increase with area for small and medium-sized family agricultural farms. An increase in the Eco-Efficiency index with an increase in farm area leads to a suspicion that the smaller the farm area is, the more extensive the agricultural production being carried out. In addition, an increase in human capital efficiency with an increase in the area of a farm indicates that there is inefficiency in the utilisation of human capital resources in the agricultural farms studied.

Sustainable Development Goals (SDGs) envisaged under Agenda 2030 are a set of seventeen goals which envisage a holistic approach towards attaining certain targets keeping humankind and the planet at center. There are total 169 targets spread across seventeen goals covering wide ranging issues and challenges the world is facing in the twenty-first century. And they are to be achieved by 2030. Concerted efforts of all the stakeholders ranging from indigenous communities, common citizens, scientists, policy makers, world leaders are needed to achieve all the goals and targets Of the seventeen goals, at least seven goals are of interest to the ethnobotanists and are associated with traditional ethnobotanical knowledge. Therefore to achieve those set of goals, a thorough understanding is required to disentangle the intricacies involving traditional ethnobotanical knowledge, indigenous people as traditional knowledge holders and their future role. Understanding relationships between traditional ethnobotanical knowledge and indigenous communities, seeking cooperation from and establishing partnerships with them would help us design policies to achieve intended outcomes of SDGs. In this paper, particular attention is attracted towards the potential role of traditional ethnobotanical knowledge in achieving select sustainable development goals and targets.

Dynamics of human populations can be affected by various socio-economic factors through their influence on the natality and mortality rates, and on the migration intensity and directions. In this work we study an economic-demographic model which takes into account the dependence of the wealth production rate on the available resources. In the case of nonlocal consumption of resources the homogeneous in space wealth-population distribution is replaced by a periodic in space distribution for which the total wealth increases. For the global consumption of resources, if the wealth redistribution is small enough, then the homogeneous distribution is replaced by a heterogeneous one with a single wealth accumulation center. Thus, economic and demographic characteristics of nonlocal and global economies can be quite different in comparison with the local economy.

This study assesses Human Thermal Comfort in two selected areas: a Green Infrastructure (GI) area represented by a garden and a high-rise building area, in the Central Business District (CBD) of Melbourne, Australia. Three-dimensional microclimatic modelling software, ENVI-met version 4 was used to simulate the microclimate. The indices of Predicted Mean Vote (PMV), Physiological Equivalent Temperature (PET) and Universal Temperature Climate Index (UTCI) were used to quantify the level of thermal comfort in the research areas. The simulation results showed that at midday, the difference in temperature between the garden area and the high-rise building area was approximately 1°C. Increasing temperatures at midday led to a change in the level of thermal comfort for both the areas, even though it was not significant. In general, the thermal perception in the GI area was slightly ‘cooler’ than in the high-rise building area. The results of the study indicated the important role of GI in improving the thermal comfort in urban areas.

The purpose of this study is to explore the effects of dual-task training, including cognitive tasks, on cognitive and bodily functioning and β-amyloid levels in Alzheimer's dementia patients. The subjects were 34 inpatients diagnosed with Alzheimer's dementia at a nursing hospital located in Gyeongsansi, South Korea. The patients were randomly divided into a dual-task group (n = 16) and a single-task group (n = 18). The dual-task group performed cognitive tasks at the same time as exercising tasks, while the single-task group performed only exercise tasks. Each group was trained for 30 minutes three times a week for eight weeks. The Mini-Mental State Examination was used to measure the patients’ cognitive function. Static and dynamic balance were measured to evaluate bodily functioning. Static balance was measured using Biorescue, while dynamic balance was measured using the Berg Balance Scale. Blood analysis was performed to measure levels of β-amyloid, which is known to cause Alzheimer's dementia. Both groups exhibited statistically significant improvements in gait function after the training (p < .05). The dual-task group exhibited statistically significant differences in cognitive function, static and dynamic balance function, and β-amyloid levels after training (p < .05). A significant difference was observed between the two groups (p < .05). Dual-task activities were found to be effective in improving cognitive and bodily functioning and reducing β-amyloid levels in Alzheimer's dementia patients. Therefore, dual-task training is thought to be an effective method of treating and preventing Alzheimer's dementia.

Policy induced decline of human mobility has been recognised to be effective in controlling the COVID-19 spread especially in the initial stage of the outbreak, although the relationship among mobility, policy implementation, and virus spread remains contentious. Coupling data of confirmed COVID-19 cases with Google mobility data in Australia, we present a state-level empirical study to: 1) inspect the temporal variation of COVID-19 spread and the change of mobility adherent to social restriction policies; 2) examine the extent that different types of mobility are associated with the COVID-19 spread in eight Australian states/territories; and 3) analyse the time-lag effect of mobility restriction on the COVID-19 spread. We find that social restriction policies implemented in the early stage of the pandemic controlled the COVID-19 spread effectively; the restriction of human mobility has a time-lag effect on growth rates, and the strength of the mobility-spread correlation increases up to seven days after policy implementation but decreases afterwards. The association between mobility and COVID-19 spread varies across space and time, and subjects to the types of mobility. Thus, it is important for governments to consider the degree to which lockdown conditions can be eased by accounting for this dynamic mobility-spread relationship.

Eight compounds were isolated from the roots of Glycyrrhiza uralensis and tested for cholinesterase (ChE) and monoamine oxidase (MAO) inhibitory activities. Glycyrol (GC) effectively inhibited butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) with IC50 values of 7.22 and 14.77 µM, respectively, and also moderately inhibited MAO-B (29.48 µM). Six of the other seven compounds only weakly inhibited AChE and BChE, whereas liquiritin apioside moderately inhibited AChE (IC50 = 36.68 µM). Liquiritigenin (LG) potently inhibited MAO-B (IC50 = 0.098 µM) and MAO-A (IC50 = 0.27 µM), and liquiritin, a glycoside of LG, weakly inhibited MAO-B (&gt; 40 µM). GC was a reversible, noncompetitive inhibitor of BChE with a Ki value of 4.47 µM, and LG was a reversible competitive inhibitor of MAO-B with a Ki value of 0.024 µM. Docking simulations showed that the binding affinity of GC for BChE (-7.8 kcal/mol) was greater than its affinity for AChE (-7.1 kcal/mol), and suggested that GC interacted with BChE at Thr284 and Val288 by hydrogen bonds (distances: 2.42 and 1.92 Å, respectively) beyond the ligand binding site of BChE, but that GC did not form hydrogen bond with AChE. The binding affinity of LG for MAO-B (-8.8 kcal/mol) was greater than its affinity for MAO-A (-7.9 kcal/mol). These findings suggest GC and LG should be considered promising compounds for the treatment of Alzheimer’s disease with multi-targeting activities.

The development and optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells is a major challenge in clinical immunotherapy researches. According to the low transfection efficiency of conventional methods in human primary T cells, there is an effort in order to increase the transfection rate in these cells. Protamine is an FDA-approved compound with a documented safety profile that enhances DNA condensation for gene delivery. In this study, the effect of protamine on the transfection efficiency of standard transfection reagents, TurboFect, and Lipofectamine 2000 was evaluated in order to transfect primary human T cells. Results demonstrated that protamine condenses DNA and increases the positive charge of DNA/Cargo complex efficiently without any cytotoxic effect on the primary human T cells. The results also revealed that the DNA/Protamine/Cargo complexes effectively transfect human primary T cells.

It has been a long time since the world has experienced a pandemic with such a rapid devastating impact as the current COVID-19 pandemic. The causative agent, the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) is further unusual in that it appears capable of infecting many different mammal species. As a significant proportion of people worldwide are infected with SARS-CoV-2 and may spread the infection unknowingly before symptoms occur or without any symptoms ever occurring, there is a non-negligible risk of humans spreading SARS-CoV-2 to wildlife, in particular mammals. Because of SARS-CoV-2’s evolutionary origins in bats and reports of humans transmitting the virus to pets and zoo animals, regulations for prevention of human-to-animal transmission have so far focused mostly on these animal groups. Here, we summarize recent studies and reports that show that a wide range of distantly related mammals are likely susceptible to SARS-CoV-2 and that susceptibility or resistance to the virus is in general not predictable, or only to some extent, by phylogenetic proximity to known susceptible or resistant hosts. In the absence of solid evidence on the SARS-CoV-2 susceptibility/resistance for each of the >5,500 mammal species, we argue that sanitary precautions should be taken when interacting with any mammal species in the wild. Preventing human-to-wildlife SARS-CoV-2 transmission is important for protecting these (sometimes endangered) animals from disease, but also to avoid establishment of novel SARS-CoV-2 reservoirs in wild animals. The risk of repeated re-infection of humans from such a wildlife reservoir could severely hamper SARS-CoV-2 control efforts. For wildlife fieldworkers interacting directly or indirectly with mammals, we recommend sanitary precautions such as physical distancing, wearing face masks and gloves, and frequent decontamination, which are very similar to regulations currently imposed to prevent transmission among humans.

Different factors seemingly account for the emergence of present-day languages in our species. Human self-domestication has been recently invoked as one important force favouring language complexity mostly via a cultural mechanism. As a consequence, evolutionary changes impacting on aggression levels are expected to have fostered this process. Here we hypothesise about a positive effect of dog-human interactions on aggression management and more generally, on our self-domestication, ultimately, contributing to aspects of language evolution. We review evidence of diverse sort (ethological mostly, but also archaeological, genetic, and physiological) supporting a positive feedback loop between dog domestication and human-self domestication that might have favoured the mechanisms promoting structural complexity in human languages.

Road dust resuspension, especially the particulate matter fraction below 10 µm (PM10), is one of the main air quality management challenges in Europe. Road dust samples were collected from representative streets (suburban and urban) of the city of Viana do Castelo, Portugal. PM10 emission factors (mg veh-1 km-1) ranging from 49 (asphalt) to 330 (cobble stone) were estimated by means of the United Stated Environmental Protection Agency method. Two road dust fractions (< 0.074 mm and from 0.0074 to 1 mm) were characterised for their geochemical, mineralogical and morphological properties. In urban streets, road dusts reveal the contribution from traffic emissions, with higher concentrations of e.g. Cu, Zn, and Pb. In the suburban area, agriculture practices likely contributed to As concentrations of 180 mg kg-1 in the finest road dust fraction. Samples are primarily composed of quartz, but also of muscovite, albite, kaolinite, microcline, Fe-enstatite, graphite and amorphous content. Particle morphology clearly shows the link with natural and traffic related materials, with well-formed minerals and irregular aggregates. The hazard quotient suggests a probability to induce non-carcinogenic adverse health effects in children by ingestion of Zr. Arsenic in the suburban street represents a human health risk of 1.58 x 10-4.

A lot of research studies have been surveyed the completed genomes of prokaryotic and eukaryotic and focused on the correlation between the percentage of microsatellite sequences in completed genomes and the whole size of the organism genomes. There are fewer studies made in repetitive sequences otherwise simple sequence repeats or long tandem repeats of virus genomes. simple sequence repeats (SSRs) are the most important regions for recombination and moving repeats blocks from site to another site in the genomes. A tool was programmed and designed by visual basic 6.0 to find the long tandem repeats in DNA sequences of the small genomes. The tool named “Repeater Finder Regular Expression”, (RFRE) Version 1.0, 2016. The tool was utilized to discover different pattern of long tandem repeats (LTR) motifs on the completed genomes of human corona virus strains by using a joined regular expression language. In this study, a twenty-nine accession numbers of human coronavirus completed genomes, (hku1) strains were retrieved from the Genbank. The researcher can write a different regular expression patterns and joined regular expression patterns through the designed tool to search and find a specific motif of nucleotide sequences inside the complete genomes. The RFRE tool searched and found three different total lengths of a perfect long tandem repeats (240bp, 300bp and 480bp). A Dot plot gave a picture view for the long tandem repeat sequences in the completed genome sequence (KF430201.1) of human coronavirus. The genomic dot plot tool YASS was used as a genomic similarity searching tool to check for the uninterrupted repeats and confirm the sensitivity of the (RFRE) tool. To identify the recombination site in the genomes of human coronavirus the RAT tool was applied to find the recombination sites between the completed genomes of human corona viruses .The RAT tool recognized the recombination site in the nucleotide position (3012) and at the same time this recombination site position (3012) was also recognized as a beginning position of a long tandem repeat. A precise motif was predicted from the translated repeats of Human Corona Virus which found by PRATT tool. There was a relationship between the total length of long tandem repeats and genome size of Human Corona Virus and the correlation value R² was equal to (0,451). In conclusion, this study presented the importance of finding the long tandem repeats of human coronavirus and gives a relationship between the completed genome size of human coronavirus types and long tandem repeats. The nucleotide position (3012) was a hot spot site for a recombination among the complete genomes of human coronavirus and also identified as a repetitive site in the genomes of human corona virus (hku1). The repeats in human coronavirus (hku1) were predicated to be a main major role of virus evolution.

There is currently an interest in “active” implantable biomedical devices that include mechanical stimulation as an integral part of their design. This paper reports the experimental use of a porous scaffold made of interconnected networks of slender ferromagnetic fibres that can be actuated in vivo by an external magnetic field applying strains to in-growing cells. Such scaffolds have been previously characterized in terms of their mechanical and cellular responses. In this study, it is shown that the shape changes induced in the scaffolds can be used to promote osteogenesis in vitro. In particular, immunofluorescence, gene and protein analyses reveal that the actuated networks exhibit higher mineralization and extracellular matrix production, and express higher levels of osteocalcin, alkaline phosphatase, collagen type 1a1, runt-related transcription factor 2 and bone morphogenetic protein 2 than the static controls at the 3-week time point. The results suggest that the cells filling the inter-fibre spaces are able to sense and react to the magneto-mechanically induced strains facilitating osteogenic differentiation and maturation. This work provides evidence in support of using this approach to stimulate bone ingrowth around a device implanted in bone and can pave the way for further applications in bone tissue engineering.

Due to remarkable socioeconomic development, an increasing number of karst rocky desertification areas have been severely affected by human activities in southern China. Effectively analyzing human activities in karst rocky desertification areas is a critical prerequisite for managing and restoring areas with tremendous negative impacts from desertification. At present, a timely and accurate way of quantifying the spatiotemporal variations of human activities in karst rocky desertification areas is still lacking. In this communication, we attempted to quantify human activities from the corrected NPP-VIIRS nighttime light data from 2012 to 2018 based on statistical analysis. The results show that a significant increase of night lights could be clearly identified during the study period. The total nighttime lights (TL) related to severe karst rocky desertification (S) were particularly concentrated in Guizhou and Yunnan. The nighttime light intensity (LI) related to the S areas in Chongqing were the strongest due to its rapid socioeconomic development. The annual growth rate of nighttime lights (GL) has been slow or even negative in Guangdong because of its various karst rocky desertification restoration programs. This communication could provide an effective approach for quantifying human activities and provide useful information about where prompt attention is required for policy-making on the restoration of the karst rocky desertification areas.

The core components of regenerative medicine are stem cells with high self-renewal and tissue regeneration potentials. Adult stem cells can be obtained from many organs and tissues. NANOG, SOX2 and OCT4 represent the core regulatory network that suppresses differentiation-associated genes, maintaining the pluripotency of mesenchymal stem cells. The roles of NANOG in maintaining self-renewal and undifferentiated status of adult stem cells are still not perfectly established. In this study we define the effects of downregulation of NANOG in maintaining self-renewal and undifferentiated state in mesenchymal stem cells (MSCs) derived from subcutaneous adipose tissue (hASCs). hASCs were expanded and transfected in vitro with short hairpin Lentivirus targeting NANOG. Gene suppressions were achieved at both transcript and proteome levels. The effect of NANOG knockdown on proliferation after 10 passages and on the cell cycle was evaluated by proliferation assay, colony forming unit (CFU), qRT-PCR and cell cycle analysis by flow-cytometry. Moreover, NANOG involvement in differentiation ability was evaluated. We report that downregulation of NANOG revealed a decrease in the proliferation and differentiation rate, inducing cell cycle arrest by increasing p27/CDKN1B (Cyclin-dependent kinase inhibitor 1B) and p21/CDKN1A(Cyclin-dependent kinase inhibitor 1A) through p53 and regulate DLK1/PREF1. Furthermore, NANOG induced downregulation of DNMT1, a major DNA methyltransferase responsible for maintaining methylation status during DNA replication probably involved in cell cycle regulation. Our study confirms that NANOG regulates the complex transcription network of plasticity of the cells, inducing cell cycle arrest and reducing differentiation potential.

Solar Energy has enormous potential to fulfill the energy requirements of the world and can be extracted using solar cells. However, the solar cells are affected by poor efficiency and further affected by wind speed, orientation of the panel, temperature and dust deposition. There are different cleaning technologies devised by many industry experts to clean the solar panels. However, they are facing drawbacks when deployed in the solar farm. An efficient cleaning system, along with an added cooling system must be devised, so that the solar panels must be cleaned and cooled often to maximize the energy production. This paper presents a low-cost, energy-efficient, smart and innovative dust cleaning and cooling system for Photovoltaic (PV) Panels. The system is designed, fabricated, fully-automated using Programmable Logic Controller (PLC) and tested successfully. A battery-charging kiosk, capable of charging two, 24V Lead Acid Batteries embedded within this prototype, shall provide clean-energy in a sustainable manner to the rural communities of the developing nations. The user can check the status of the battery such as battery voltage, battery temperature, and state of charge on the Human Machine Interface (HMI) panel while charging the batteries

Shenzhen is China’s top ten clean air city and the cleanest air megacity. Even so, epidemiologic studies have shown ambient air pollution had significant adverse impacts on human health in this less polluted city. In this study, the concentrations of six criteria air pollutants (PM2.5, PM10, O3, NO2, SO2, and CO) from 2014 to 2017 were analyzed and compared to thresholds of both national and international air quality standards. The results showed concentrations of all air pollutants were below target values of current national air quality standard, but levels of particulate matter (PM) and O3 were still much higher than the recommended levels by the World Health Organization. Within national air quality standards, the number of over-limit days was rare with few variations between highly polluted and low pollution areas. The air quality improvement was slowing down recently. Our results suggest annual and daily thresholds for PM are too loose for air quality improvement in Shenzhen. Hence, we call for evaluation and establishment of tougher air quality standard.

Free-range sloths living in an urban environment is rare. In this study, human opinions, attitudes and interactions with a population of Bradypus variegatus in a public square were investigated. A questionnaire was applied to people in the square where the sloths reside, and informal, opportunistic observations of human-sloth interactions were made. 95% of respondents knew of the sloths’ existence in the square and 87.8% likes their presence. Opinions about population size differed greatly and younger people were concerned if the square was appropriate place for them. Some human-sloth interactions showed the consequence of lack of biological knowledge. People initiated all sloth-human interactions. The fact that sloths are strictly folivorous has limited their interactions with humans and consequently minimised negative impact of the human-animal interaction on their wellbeing. These results demonstrate that while there is a harmonious relationship between people and sloths, actions in environmental education of the square’s public could be beneficial for the sloths.

Recent advances in recording and real-time analysis of surface electromyographic signals (sEMG) have fostered the use of sEMG human-machine interfaces for controlling personal computers, prostheses of upper limbs, and exoskeletons among others. Despite a relatively high mean performance, sEMG-interfaces still exhibit strong variance in the fidelity of gesture recognition among different users. Here we systematically study the latent factors determining the performance of sEMG-interfaces in synthetic tests and in an arcade game. We show that the degree of muscle cooperation and the amount of the body fatty tissue are the decisive factors in synthetic tests. Our data suggest that these factors can only be adjusted by a long-term training, which promotes fine-tuning of low-level neural circuits driving the muscles. A short-term training has no effect on synthetic tests, but significantly increases the game scoring. This implies that it works at a higher decision-making level, not relevant for synthetic gestures. We propose a procedure that enables quantification of the gestures’ fidelity in a dynamic gaming environment. For each individual subject the approach allows identifying “problematic” gestures that decrease gaming performance. This information can be used for optimizing the training strategy and for adapting the signal processing algorithms to individual users, which could be a way for a qualitative leap in the development of future sEMG-interfaces.

In this paper we take China’s one child policy as an example and investigate its environmental impact. We develop a model for an economy using a standard overlapping generation model extended with human capital, endogenous fertility, and changing life expectancy. To model the environmental impact of economic activities, we use a modified IPAT model. We show that China’s one child has a very strong positive impact on the environment, particularly if we consider the whole human legacy.

The human body supports the growth of a wide array of microbial communities in various niches, such as the oral cavity, gastro-intestinal and urogenital tracts and on the surface of the skin. These host associated microbial communities include yet-un-cultivable bacteria and are influenced by various factors. Together, these communities of bacteria are referred to as the human microbiome. Human oral microbiome consists of both symbionts and pathobionts. Deviation from symbiosis among the bacterial community leads to “dysbiosis”—a state of community disturbance. Dysbiosis occurs due to many confounding factors that predispose to a shift in the composition and relative abundance of microbial communities. Dysbiotic communities have been a major cause for many microbiomes related systemic infections. Such dysbiosis is directed by certain important pathogens called the “keystone pathogens” that could modulate community microbiome variations. One such persistent infection is oral infection, mainly periodontitis, where a wide array of causal organisms has been implied to systemic infections such as cardio vascular disease, diabetes mellitus, rheumatoid arthritis and Alzheimer’s disease. The keystone pathogens co-occur with many yet-cultivable bacteria and their interactions lead to dysbiosis. This has been the focus of recent research. While immune evasion is one of the major modes that lead to dysbiosis, new processes and new virulence factors of bacteria have been shown to be involved in this important process of that determine disease or health state. This review focuses on such dysbiotic communities, their interactions and their virulence factors that predispose the host to other systemic implications.

The objective is to study the local perceptions on the conservation of wild chimpanzees (Pan troglodytes verus) in the Réserve Naturelle Communautaire de Dindéfélo (RNCD), southeast of Senegal, to design specific actions to improve conservation management. We conducted 338 semi-structured interviews in three main villages of RNCD. Three-fourths of the population were farmers. Of those interviewed, 29% received elementary education. Two of the three villages participated in a project to plant trees as fences. On average, 66% of the respondents were animists. Of the respondents who were afraid to see a chimpanzee, 68% answered because they attack. Seventy-seven percent washed their clothes in the forest river because there was more water than in the village wells. Of the interviewees who threw the old clothes into the forest, 50% did it due to tradition. Ninety-six percent of respondents stated that chimpanzees do not feed from their crops. The main problems of the locals with the Reserve were lack of water and basic resources and not been allow to cut trees in protected areas. There were significant relationships between education (1 relationship), environmental project (4 relationships) and animism (11 relationships) with local perceptions. The 93% of the respondents who had the traditional belief that “if the old clothes were burnt, children would become sick” feel fear of chimpanzees, while those who did not have this animistic belief the 6% are afraid (χ² = 1.57, P < 0.02). These local perceptions allow us to design specific course of action to improve chimpanzee conservation and sustainable coexistence in this complex period of the Anthropocene.

In most industrialized countries, screening programs for cervical cancer have shifted from cytology (Pap smear or ThinPrep) alone on clinician-obtained samples to the addition of screening for human papillomavirus (HPV), its main causative agent. For HPV testing, self-sampling instead of clinician-sampling has proven to be equally accurate, in particular for assays that use nucleic acid amplification techniques. In addition, HPV testing of self-collected samples in combination with a follow-up Pap smear in case of a positive result is more effective in detecting precancerous lesions than a Pap smear alone. Self-sampling for HPV testing has already been adopted by some countries, while others have started trials to evaluate its incorporation into national cervical cancer screening programs. Self-sampling may result in more individuals willing to participate in cervical cancer screening, because it removes many of the barriers that prevent women, especially those in low socioeconomic and minority populations, from participating in regular screening programs. Several studies have shown that the majority of women who have been underscreened but who tested HPV-positive in a self-obtained sample, will visit a clinic for follow-up diagnosis and management. Additionally, a self-collected sample can also be used for vaginal microbiome analysis, which can provide additional information about HPV infection persistence as well as vaginal health in general.

Clinical rehabilitation and sports performance analysis both require the objectification of movement. Kinovea^© is a free 2D motion analysis software that enables the establishment of kinematics parameters. This low-cost technology has been used in sports sciences, as well as clinical field and research work. Although it has been validated as a tool with which to assess time-related variables, this is not yet the case regarding angular and distance variables. The main objective of this study was to determine the validity and reliability of the Kinovea software in obtaining angular and distance data at different perspectives of 90°, 75°, 60° and 45°. For this purpose, a figure with 29 points was designed (in AutoCAD) and 24 frames analysed. Each frame was examined by three observers who each made two attempts. For each export data item, 20 angles and 20 distance variables were calculated, with intra- and inter-observer reliability also analysed. To evaluate Kinovea reliability and validity a multiple approach was applied involving the following analysis: -systematic error with a two-way ANOVA 2x4; -relative reliability with ICC and CV (95% confidence interval); -absolute reliability with Standard Error. The results thus obtained indicate that the Kinovea software is a valid and reliable tool that is able to measure accurately at distances up to 5 m from the object and at an angle range of 90°–45°. Nevertheless, for optimum results an angle of 90° is suggested.

Praeruptorin A(PA), a naturally existing pyranocumarin, is isolated from the dried root of Peucedanum praeruptorum Dunn. So far the anti-cancer effect and molecular mechanism behind Praeruptorin A action in human cervical cancer HeLa cells remain unknown. In the present study, we find that PA reduces cell proliferation and colony formation of human cervical cancer HeLa cells through inducing cell cycle arrest at G0/G1 phase. PA-upregulated p21 and p27 proteins are observed, accompanied with inhibition of cyclin D1 and S-phase kinase-associated protein 2(Skp2) proteins expression. PA could significantly inhibit migration and invasion of human HeLa cells. Meanwhile, PA significantly reduces invasive protein expression of matrix metalloproteinase-2 (MMP-2), and increases protein expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). PA is observed to possess the capacity in suppressing ERK1/2 activation. PD98059 (ERK specific inhibitor) significantly enhances PA-induced downregulation of MMP-2 expression, and upregulation of TIMP-2 expression. Moreover, we found that PA treatment notably inhibits 12-O-tetradecanoylphorbol-13-acetate(TPA)-upregulated ERK1/2 activation, MMP-2 expression, cellular migration and invasion in human HeLa cells. Taken together, these findings are the first to demonstrate the anti-cancer activity of PA, which may act as a promising therapeutic agent for the treatment of human cervical cancer.

Sinulariolide, a natural product extracted from cultured-type soft coral Sinularia flexibilis, possesses bioactivity against the movement of several types of cancer cell. However, the molecular pathway of its effects on human bladder cancer remain poorly understood. Using a human bladder cancer cell line as an in vitro model, this study investigated the underlying mechanism of sinulariolide against cell migration/invasion in TSGH-8301 cells. We found that sinulariolide inhibited TSGH-8301 cell migration/invasion, and the effect was concentration-dependent. Furthermore, the protein expressions of matrix metalloproteinases (MMPs) MMP-2 and MMP-9, as well as urokinase, were significantly decreased after 24-h sinulariolide treatment. Meanwhile, the increased expressions of tissue inhibitors of metalloproteinases (TIMPs) TIMP-1 and TIMP-2 were in parallel with an increased concentration of sinulariolide. Finally, the expressions of several key phosphorylated proteins in the mTOR signaling pathway were also downregulated by sinulariolide treatment. Our results demonstrated that sinulariolide has significant effects against TSGH-8301 cell migration/invasion, and its effects were associated with decreased levels of MMP-2/-9 and urokinase expression, as well as increased TIMP-1/TIMP-2 expression. The inhibitory effects were mediated by reducing phosphorylation proteins of the PI3K, AKT and mTOR signaling pathway. The findings suggested that sinulariolide is a good candidate for advanced investigation with the aim of developing a new drug for the treatment of human bladder cancer.

Human visual system (HVM) can quickly localize the most salient object in scenes, which has been widely applied on natural image segmentation [15]-[19]. In ultrasound (US) breast images, compared with background areas, tumor is more salient because of its higher contrast. In this paper, we develop a novel automatic localization method based on HVM for automatic segmentation of ultrasound (US) breast tumors. First, the input image is smoothed by convolution with a linearly separable Gaussian filter and then subsampled into a 9-layer Gaussian pyramid. Then intensity, blackness ratio, and superpixel contrast features are combined to compute saliency map, in which Winner Take All algorithm is used to localize the most salient region, presenting with a circle on the localized target. Finally the circle is taken as the initial contour of CV level set to finish the extraction of breast tumor. The localization method has been tested on 400 US beast images, among which 378 images have higher saliency than background areas and succeed in localization, with high accuracy 92.00%. The HVM localization method can be used to localize the tumors, combined with this method, CV level set can achieve the fully automatic segmentation of US breast tumors. By combing intensity, blackness ratio and superpixel contrast features, the proposed localization method can successfully avoid the interference caused by background areas with low echo and high intensity. Moreover, multi-object localization of US breast images can be considered in future employment.

Artisanal and small-scale gold mining (ASGM) has been an important source of income for communities in the Madre de Dios River Basin in Peru for hundreds of years. However, in recent decades, the scale of ASGM activities in the region has increased dramatically, and exposures to a variety of occupational and environmental hazards related to ASGM, including mercury, are becoming more widespread. The aims of our study were to: (1) examine patterns in the total hair mercury level of human participants in several communities in the region and compare these results to the 2.2 µg/g total hair mercury level equivalent to the World Health Organization (WHO) Expert Committee of Food Additives (JECFA)’s Provisional Tolerable Weekly Intake (PTWI); and (2), to measure the mercury levels of paco (Piaractus brachypomus) fish raised in local aquaculture ponds, in order to compare these levels to the EPA Fish Tissue Residue Criterion of 0.3 µg Hg/g fish (wet weight). We collected hair samples from 80 participants in four communities (one control and three where ASGM activities occurred) in the region, and collected 111 samples from fish raised in 24 local aquaculture farms. We then analyzed the samples for total mercury. Total mercury levels in hair were statistically significantly higher in the mining communities than in the control community, and increased with increasing distance away from the Madre de Dios headwaters (as the crow flies), did not differ by sex, and frequently exceeded the reference level. Regression analyses indicated that higher hair mercury levels were associated with residence in ASGM communities. The analysis of paco fish samples found no samples that exceeded the EPA tissue residue criterion. Collectively, these results align with other recent studies showing that ASGM activities are associated with elevated human mercury exposure. The fish farmed through the relatively new process of aquaculture in ASGM areas appeared to have little potential to contribute to human mercury exposure. More research is needed on human health risks associated with ASGM to discern occupational, residential, and nutritional exposure, especially through tracking temporal changes in mercury levels as fish ponds age, and assessing levels in different farmed fish species. Additionally, research is needed to definitively determine that elevated mercury levels in humans and fish result from the elemental mercury from mining, rather than from a different source, such as the mercury released from soil erosion during deforestation events from mining or other activities.

The paper is concerned with the problem of assigning value to a transport service that moves a mass M with velocity vAB in a time τAB from a first point A to a more distant point B. It is argued that the value of such a transport service can be measured in an objective and physically accessible way by calculating the physical action WAB(M,vAB,τAB) upon moving a mass M along a specific path joining points A and B. In the specific case of a linear straight-line motion in a force-free field this action attains a minimum value of WAB_ideal= 12MvAB2τAB=12MvABLAB which shows that the function WAB_ideal(M,LAB,τAB) contains all those quantities which in practice determine the money-value of the related transport service. It is argued that with the sensor hardware routinely available in modern cars, the accumulation of transport value with driving can be continuously monitored inside a car. Such accumulation allows the transport value gained at the final destination B to be compared to the intrinsic value of a transport service that connects points A and B. In this way, the potential for optimizing transport services with regard to economic and ecological constraints can be quantitatively assessed and possible improvements be identified.

This study evaluates how automation and digital transformation affect Human Resource Development (HRD) strategies, focusing on AI-driven training programs, the effectiveness of remote work technologies, and the challenges related to digital transformation fatigue. Employing a mixed-methods approach that combines quantitative surveys (n=500) and qualitative interviews (n=30), the study offers comprehensive insights. Key findings reveal that 75% of respondents noted improved skill acquisition through AI, 60% expressed satisfaction with remote work technologies despite some challenges, and 55% reported experiencing digital transformation fatigue. The study suggests strategic investments in AI, robust remote work infrastructure, and wellness programs to mitigate fatigue, emphasizing the importance of balancing technological advancements with employee well-being.

Electronic sports (esports) and research on this emerging field are interdisciplinary in nature. By extension, it is essential to understand how to standardize and structure training with the help of existing tools developed by years of research in sports sciences and informatics. Our goal in this article was to verify if the current body of research contains substantial evidence of the training systems applied to training esports players. To verify the existing sources, we have applied a framework of scoping review to address the search from multiple scientific databases with further local processing. We conclude that the current research on esports dealt mainly with describing and modeling performance metrics spanned over multiple fragmented research areas (psychology, nutrition, informatics), and yet these building blocks were not assembled into an existing well-functioning theory of performance in esports by providing exercise regimes, and ways of periodization for esports.

This essay examines the complex interplay between human rights developments in Saudi Arabia and the country's Vision 2030 initiative, which aims to modernize its social, economic, and political landscape. The research question posed is: How are human rights being addressed within the framework of Vision 2030? The goal of the essay is to provide a qualitative analysis of the reforms and policies emerging under Vision 2030, highlighting both advancements and ongoing challenges. Key discussions include historical human rights perspectives, specific strategies aimed at enhancing women’s rights and labor rights, and the implications of legal reforms. The essay draws upon a range of scholarly sources to underscore the intricate relationship between human rights improvements and socio-economic objectives in Saudi Arabia. The main conclusions suggest that while significant strides have been made, particularly in women's empowerment and labor rights, substantial obstacles—rooted in cultural norms and institutional practices—persist. Ongoing international scrutiny and a robust domestic dialogue on human rights remain critical for the success of these reforms. The essay ultimately advocates for sustained efforts to uphold human rights as a cornerstone of the Vision 2030 agenda.

Human papillomavirus (HPV) is the most common sexually transmitted infection in the US and the world. Infection with high-risk oncogenic HPV strains have been shown to induce cellular transformation leading to anogenital and oropharyngeal cancers. The HPV vaccine first developed in 2006 for females aged 9-26 years has been demonstrated to be safe and effective in preventing 90% of all HPV associated cancers. However, vaccine hesitancy, misinformation, and barriers to vaccine access has resulted in suboptimal vaccination rates among adolescent populations, especially in rural communities in the South. HPV vaccine coverage in Tennessee is currently below the national average and below the Healthy People 2030 goal of an 80% vaccination rate for individuals 13-17 years old based on recommendation guidelines for up-to-date HPV vaccination status as of 2022. HPV vaccination rates for Tennesseans with private insurance in 2022 was 68% and 38% for those that were insured. Up-to-date HPV vaccination rates for Tennesseans living in urban communities was 58% in 2022 and 46% for those living in rural communities. Overall, HPV associated cancers rates are higher in Tennessee at 12.9/100,000 compared to the overall rate in the US of 11.8/100,000 persons in 2022. Interventions to improve HPV vaccine awareness, education, and access could improve vaccine confidence and uptake especially among rural and uninsured populations in Tennessee. Most recently, the Advisory Committee on Immunization Practices (ACIP) has expanded recommendations for the HPV vaccinations for some individuals 27-45 years who were not vaccinated at a younger age with shared clinical decision-making. The impact of this recommendation on Tennesseans should be examined.

Background: Two explored EDA1 variants regulate the biological behaviors of human dental pulp stem cells (hDPSCs), such as proliferation and migration, as well as their effects on odontogenic differentiation, providing a theoretical basis for further exploration of the mechanisms underlying EDA1-induced tooth development. Methods: The experimental groups included a wild-type EDA1 group (Wt), non-syndromic tooth agenesis variants EDA1 group (NSTA-A259E, NSTA-S374R), syndrome type variant EDA1 group (STA-H252L), and an empty vector control group (PCR3/NC). CCK-8 method was used to investigate the proliferative activity of hDPSCs. The cell cycle distribution of hDPSCs was detected by flow cytometry; The cell migration ability was assessed using the cell scratch and Transwell tests; Odontogenic differentiation medium was used to induce mineralization of hDPSCs, followed by statistical analysis. RNA-Seq revealed that FOS, JUN and other genes were found to be enriched in MAPK signaling pathway and osteoclast differentiation pathway. qPCR was used to detect mRNA expression of hub genes (FOS and JUN). Results: Compared with the NSTA-EDA1 and STA-EDA1, Wt-EDA1 promotes the proliferation and transformation of hDPSCs from the G0/G1 phase to S phase (p<0.01), with no significant difference between NSTA-EDA1 and STA-EDA1 (p>0.05). Wt-EDA1 also upregulated hDPSCs cell migration compared to NSTA-EDA1 and STA-EDA variants, as demonstrated by migration assays (p<0.01). NSTA-EDA1 and STA-EDA1 variants inhibited the odontogenic differentiation of hDPSCs compared to the Wt group, as shown by Alizarin red staining and ALP staining (p<0.01). RNA sequencing revealed that the differentially expressed genes FOS and JUN were enriched in the MAPK signaling pathway and osteoclast differentiation. Compared with Wt-EDA1, EDA1 variants lost the ability to promote c-FOS mRNA expression, as confirmed by qPCR. Conclusions: EDA1 variants fail to the promote c-FOS mRNA expression in hDPSCs, thereby inhibiting odontogenic differentiation and losing the ability to enhance the proliferation of hDPSCs.