Search | Preprints.org

Preprint ARTICLE | doi:10.20944/preprints202305.1804.v1

Targeting M2-TAM via STAT3/NF-kB/AKT Signaling Pathway With Oxaliplatin, Retinoic Acid, and Libidibia ferrea -Loaded Extracellular Vesicles From Macrophages 1 Down-Regulates Murine Colon Cancer Metastasis

Thaís Gomes De Carvalho, Pablo Lara, Carla Jorquera-Cordero, Timo Schomann, Cícero Flávio Soares Aragão, Artur de Santana Oliveira, Vinicius Barreto Garcia, Shirley Vitória de Paiva Souza, Isabelle de Lima Marques, Luiz Alberto Lira Soares, Paulo Marcos Da Matta Guedes, Raimundo Fernandes De Araújo-Júnior

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: EVs; Macrophages 1; STAT3, AKT; NF-κB; metastasis; colorectal cancer.

Online: 25 May 2023 (10:48:17 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Despite the numerous advances in target therapy for the treatment of colorectal cancer, aggressive colorectal cancer remains an incurable disease whose negative modulation of the immune system in the tumor microenvironment is still critical for improving the patient's prognosis. Extracellular vesicles (EVs) have received attention for their use as cell-membrane-camouflaged nanoparticles and drug delivery systems in nanomedicine derived by nearly all cell types for intercellular communication and regulation in the tumor microenvironment (TME). In this study, M1 Macrophage EVs (M1EVs) were used as nanocarriers of oxaliplatin (M1EV1) associated with retinoic acid (M1EV2) and Libidibia ferrea (M1EV3), or combined together with retinoic acid and Libidibia ferrea (M1EV4) to evaluate their antiproliferative and immunomodulatory potential on CT-26 and MC-38 colorectal cancer cell lines, as well as their capacity to prevent metastases in mouse models of colorectal cancer such as allographic and peritoneal. Tumors were evaluated by qRT-PCR and immunohistochemistry. The cell death profile and epithelial-mesenchymal transition process (EMT) were analyzed in vitro in colorectal cancer cell lines. Polarization of murine macrophages (RAW264.7 cells) was also carried out. M1EV2 and M1EV3 used alone or especially combined (M1EV4) downregulated the tumor progression by TME immunomodulation, leading to a decrease in primary tumor size and metastases in peritoneum, liver and lungs. STAT3, NF-kB and AKT were the major genes downregulated by systems of M1EVs. Tumor-associated macrophages (TAM) switched M2 phenotype (CD163) towards M1 (CD68) reducing levels of IL-10, TGF-β and CCL22. Furthermore, malignant cells showed overexpression of FADD, APAF-1, caspase-3, and E-cadherin, and decreased expression of MDR-1, survivin, vimentin, CXCR4, and PD-L1 after treatment with systems of M1EVs. The results obtained in this study provided evidence that EVs from M1 antitumor macrophages can transport drugs and increase their immunomodulatory and antitumor activity by stimulating activation or blockage of pathways involved in cell proliferation, migration, cell survival, and drug resistance processes.

Preprint ARTICLE | doi:10.20944/preprints202304.0473.v1

On the Evidence of the European Bee-Eater (Merops apiaster) as a Predator of the Yellow-Legged Hornet (Vespa velutina) and its Potential Contribution as a Biological Control Agent

Nuno Xavier Roncon Soares Onofre, Maria Inês Perestrello de França Portugal e Castro, Anabela Cristina Marques Nave, Irene S.P. Cadima, Maria José Souza Costa Ferreira, Joana Godinho

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: ; yellow-legged hornet; European bee-eater; biological control; predation; sympatry ;

Online: 18 April 2023 (03:51:26 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202304.0346.v1

The Influence of the Nature of the Dispersing Agent on the Interaction of DNA Nucleotides with the Surface of YSZ Nanoparticles and the Homogeneity of Lyophilites Based on Them

Aleksander S. Doroshkevich, Viktor S. Doroshkevich, Tatyana Yu. Zelenyak, Nelya V. Doroshkevich, Andriy I. Lyubchyk, Sergiy I. Lyubchyk, Svitlana B. Lyubchyk, Alisa A. Tatarinova, Anca Stanculescu, Dan Chicea, Carmen Mita, Nicoleta Cornei, Diana Mardare, Boris L. Oksengendler, Nadejda N. Nikiforova, Matlab N. Mirzayev, Evgeni P. Popov, Alexander A. Donkov, Vesna Teofilović, Bozena Jasinska, Le Hong Khiem

Subject: Chemistry And Materials Science, Other Keywords: immobilization of DNA molecules on the surface of dielectrics; interaction of nanopowders with nucleotides; molecular electronics; nanotechnology; molecular dynamics modeling; DNA conformation in an electric field; genotoxic effects

Online: 14 April 2023 (04:03:43 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202304.0125.v1

De Novo Venom Gland Transcriptome Assembly and Characterization for Calloselasma rhodostoma (Kuhl, 1824), the Malayan Pit Viper from Malaysia: Unravelling Toxin Gene Diversity in a Medically Important Basal Crotaline

Choo Hock Tan, Kae Yi Tan, Tzu Shan Ng, Nget Hong Tan, Ho Phin Chong

Subject: Biology And Life Sciences, Life Sciences Keywords: kistomin; rhodostoxin; rhodostomin; rhodocytin; rhodocetin; ancrod

Online: 7 April 2023 (11:36:15 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202303.0176.v1

Higher Penetration Depth of 1064 NM Compared to 905 NM Laser Light Into Biological Tissue Is Only Relevant for Surface Layers and Insignificant for 10 MM Tissue Depth Onwards

Leon Kaub, Christoph Schmitz

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: laser therapy; musculoskeletal system; tissue penetration depth

Online: 9 March 2023 (11:03:09 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202301.0035.v2

Estimating Prevalence of Bereavement, Its Contribution to Risk for Binge Drinking, and Other High-Risk Health States in a State Population Survey, 2019 Georgia Behavioral Risk Factor Surveillance Survey

Toni Miles, Changle Li, M. Mahmud Khan, Rana Bayakly, Deborah Carr

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: BRFSS; Binge Drinking; Population Survey; Bereavement; SDG#3; Mental Health

Online: 22 February 2023 (14:30:30 CET)

Show abstract| Download PDF| Share

Binge drinking is a pattern of alcohol abuse. Its prevalence and associated risk factors are not well documented. Heavy drinking, on the other hand, has a well-studied association with bereavement . This report uses a cross-sectional, population-based survey to estimate prevalence of bingeing and its association with new bereavement. Bingeing is defined as 4 or more drinks (women) or 5 or more drinks (men) in a 2-to-4-hour setting. For the first time, the Georgia Behavioral Risk Factor Surveillance Survey (BRFSS) included a bereavement item, ‘Have you experienced the death of a family member or close friend in the years 2018 or 2019?’. Methods: Georgia BRFSS is a complex sampling survey administered annually. It is designed to represent the 8.1 million persons in the U.S. state of Georgia aged 18 years and older. Alcohol consumption patterns are routinely measured in the common core. In the 2019 field survey, the state added a new item probing for bereavement in the prior 24 months predating the pandemic of COVID-19. Imputation and weighting techniques were applied to yield population prevalence rates of new bereavement, bingeing, and their co-occurrence with other high-risk health states. Models, adjusted for age, gender, and race were used to estimate the risk for other unhealthy behaviors posed by the co-occurrence of bereavement and bingeing. Results: In Georgia, bereavement is common (45.8 %), and alcohol consumption is common (48.8 %). Bereavement and alcohol use co-occurred among 1,796,817 persons (45 % of all drinkers). The subset of co-occurring bereavement and binging totaled 608,282 persons. Within this group, the highest rates of bereavement were associated with death of a friend / neighbor (30.7%) or 3 plus deaths (31.8%). Conclusions: While bingeing is a known risk to public health, its co-occurrence with recent bereavement is a new observation. Public health surveillance systems need to monitor this co-occurrence to protect both individual and societal health. In a time of global bereavement, documenting its influence on binge drinking can support the work towards Sustainable Goal #3 – Good health and Well-Being. Keywords: BRFSS, Binge Drinking, Population Survey, Bereavement, SDG#3. Mental Health

Preprint BRIEF REPORT | doi:10.20944/preprints202212.0518.v1

Epitope Coverage of Anti-SARS-CoV-2 Nucleocapsid IgA and IgG Antibodies Correlates With Protection Against Re-Infection by New Variants in Subsequent Waves of the COVID-19 Pandemic

Michelle O. Mullins, Muneerah Smith, Hazel Maboreke, Andrew J. M. Nel, Ntobeko A. B. Ntusi, Wendy A. Burgers, Jonathan M. Blackburn

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: immunoassay; epitope coverage; quantitative antibody binding; protein microarray; SARS-CoV-2 antibodies; humoral response; SARS-CoV-2 re-infection

Online: 27 December 2022 (10:43:18 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202211.0545.v1

Genotype Diversity, Wild Bird-to-Poultry Transmissions, and Farm-to-Farm Carryover during the Spread of the Highly Pathogenic Avian Influenza H5N1 in the Czech Republic in 2021/2022

Alexander Nagy, Martina Stará, Lenka Černíková, Lada Hofmannová, Kamil Sedlák

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: H5N1; HPAI; avian influenza; highly pathogenic avian influenza; outbreak; poultry

Online: 29 November 2022 (09:11:22 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202211.0455.v1

Estimating the Social Costs of Child Abuse in Residential Care for Children with Disabilities using the Japanese Survey on the Interactions of Adverse and Positive Childhood Experiences toward Adulthood

Naruhisa Nakane, Ichiro Wada

Subject: Social Sciences, Sociology Keywords: child maltreatment; child abuse, social costs of child abuse; residential care for children with disabilities; Japan

Online: 24 November 2022 (07:32:25 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202209.0245.v2

Tracing the Food Web of Changing Arctic Ocean: Trophic Status of Highly Abundant Fish, Gasterosteus aculeatus (L.), in the White Sea Recovered Using Stomach Content and Stable Isotope Analyses

Anna S. Genelt-Yanovskaya, Natalia V. Polyakova, Mikhail V. Ivanov, Ekaterina V. Nadtochii, Tatiana S. Ivanova, Evgeny A. Genelt-Yanovskiy, Alexei V. Tiunov, Dmitry L. Lajus

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: threespine stickleback; Gasterosteus aculeatus; stomach content analysis; stable isotope analysis; fish diet; the White Sea; boreal fish; Subarctic

Online: 31 October 2022 (02:13:07 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0230.v1

Refusal Parenting Style Is Easier for Students to Perceive Non-physical Bullying in the Upper Grades of Elementary School: Mediation Effect of Negative Affect Experience and Negative Coping Style

Houyu Zhou, Qinfei Wang, Shuxu Yu, Quangquang Zheng

Subject: Social Sciences, Psychology Keywords: perceiving campus non-physical bullying; Refusal parenting style; negative affect experiences; negative coping style; mediating effect

Online: 17 January 2022 (14:09:27 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202111.0132.v2

Relative Effectiveness of Cell-Cultured versus Egg-Based Seasonal Influenza Vaccines in Preventing Influenza-Related Outcomes in Subjects 18 Years Old or Older: A Systematic Review and Meta-Analysis

Joan Puig-Barberà, Sonia Tamames-Gómez, Pedro Plans-Rubio, José María Eirós-Bouza

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: adults; influenza; cell-cultured vaccine; egg-based vaccine; influenza vaccine; relative vaccine effectiveness; real word evidence; mutation; human / prevention &; control*; comparative study

Online: 20 December 2021 (11:00:26 CET)

Show abstract| Download PDF| Supplementary Files| Share

Working Paper ARTICLE

Impact of Sacubitril/Valsartan on Indication of Implantable Cardioverter Defibrillator, Left Ventricle Ejection Fraction and Biomarkers: Data From a Real Life Cohort in Heart Failure With Reduced Ejection Fraction

José Ignacio Morgado García de Polavieja, Andrea Sigismondi, Samuel Ortiz Cruces, José Francisco Díaz Fernández

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Heart failure with reduced ejection fraction; Real-life practice; Sacubitril/valsartan; Left ventricular ejection fraction recovery; Implantable cardioverter defibrillator

Online: 5 May 2021 (10:30:13 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202104.0210.v1

A Solution of the Cosmological Constant and DE Using Breakup of Primordial Black Holes, Via a Criteria Brought Up by Dr. Freeze Which Initiates DE as Linked to Inflation

Andrew Beckwith

Subject: Physical Sciences, Acoustics Keywords: Minimum scale factor; cosmological constant; space-time bubble; bouncing cosmologies

Online: 7 April 2021 (15:25:45 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202102.0390.v1

Bioassay-Guided Isolation of 2-[P-(2-Carboxyhydrazino)Phenoxy]-6-(Hydroxymethyl)Tetrahydro-2h-Pyran-3,4,5-Triol From Oroxylum Indicum and Investigation of Its Molecular Mechanism Action of Apoptosis Induction

Asem Robinson Singh, Salam Asbin Singh, Thanngjam Davis Singh, Naorem Tarundas Singh, Takhellambam Machathoibi Chanu, Okram Mukherjee Singh, Lisam Shanjukumar Singh

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Oroxylum indicum; Oroxyquinone; Traditional medicine; Bioassay Guided Fractionation; caspase-independent apoptosis; anti-metastatic

Online: 17 February 2021 (12:55:57 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202101.0224.v1

Synthesizing Multi-Layer Perceptron Network with Ant Lion, Biogeography-Based, Dragonfly Algorithm, Evolutionary Strategy, Invasive Weed, and League Champion Optimization Hybrid Algorithms in Predicting Heating Load in Residential Buildings

Hossein Moayedi, Amir Mosavi

Subject: Engineering, Automotive Engineering Keywords: energy-efficient building; heating load; neural computing; biogeography-based optimization

Online: 12 January 2021 (14:46:29 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

The Feasibility of Patient-Specific Circulating Tumor DNA Monitoring Throughout Multi-Modality Therapy for Locally Advanced Esophageal and Rectal Cancer: A Potential Biomarker for Early Detection of Subclinical Disease

Christopher Boniface, Christopher Deig, Carol Halsey, Taylor Kelley, Michael B. Heskett, Charles R. Thomas Jr., Paul T. Spellman, Nima Nabavizadeh

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: liquid biopsy; ctDNA; cell free DNA; non-operative management; neoadjuvant therapy

Online: 10 December 2020 (12:52:27 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202010.0079.v1

The Effects of an 8-weeks in-season Loaded Plyometric Exercise by Elastic Band Training Program on the Peak Power, Strength, and Throwing Velocity of Junior Male Handball Players

Ghaith Aloui, Souhail Hermassi, Nicola Luigi Bragazzi, Mehrez Hammami, Yosser Cherni, Nawel Gaamouri, Roy J. Shephard, Lawrence D. Hayes, Mohamed Souhaiel Chelly

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Stretch-shortening cycle; Peak power; Plyometric with load; Team sports; Throwing.

Online: 5 October 2020 (12:11:13 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202010.0021.v1

Pioglitazone is Associated with Lower Major Adverse Cardiovascular and Cerebrovascular Events than DPP4-Inhibitors in Diabetic Patients with End-Stage Renal Disease: A Taiwan Nationwide Cohort Study, 2006-2016

Min-Hao Lin, Huang-Yu Yang, Chieh-Li Yen, Chao-Yi Wu, Chang-Chyi Jenq, George Kuo, Wei-Sheng Peng, Jia-Rou Liu, Ya-Chung Tian, Chih-Wei Yang, Gerard Anderson, Lai-Chu See

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Pioglitazone; DPP4-inhibitor; ESRD; Cardiovascular outcome; Mortality

Online: 1 October 2020 (15:41:50 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202009.0052.v1

Unique Existence of Globally Asymptotical Input-to-State Stability of Positive Stationary Solution for Impulsive Gilpin-Ayala Competition Model with Diffusion and Delayed Feedback under Dirichlet Zero Boundary Value

Ruofeng Rao

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Gilpin-Ayala competition model; globally asymptotical stability; Lyapunov function; Markovian jumping

Online: 3 September 2020 (04:26:14 CEST)

Show abstract| Download PDF| Share

Preprint CASE REPORT | doi:10.20944/preprints202009.0009.v1

Efficacy of Double Dose Dapsone Combination Therapy in the Treatment of Chronic Lyme Disease/Post-Treatment Lyme Disease Syndrome (PTLDS) and Associated Co-infections: A Report of Three Cases

Richard Horowitz, Phyllis R. Freeman

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Lyme disease; Post-Treatment Lyme Disease Syndrome (PTLDS); dapsone combination therapy (DDS CT); double dose dapsone combination therapy (DDD CT); babesiosis; persistent infection

Online: 1 September 2020 (11:30:52 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202005.0273.v2

The Frequency of Combined IFITM3 Haplotype Involving the Reference Alleles of Both rs12252 and rs34481144 is in Line with COVID-19 Standardized Mortality Ratio of Ethnic Groups in England

Dimitris Nikoloudis, Dimitrios Kountouras, Asimina Hiona

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: COVID-19; SARS-CoV-2; pandemic; IFITM3; rs12252; rs34481144; ethnic groups; bame; cytokine storm

Online: 20 August 2020 (04:29:43 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202008.0408.v1

Assessment of the Presence of Symptoms, Individual Protection Measures (IPM) and Suspect Screening Measures (SSM) of COVID-19 in Federal Police Officers from a Regional Police Station in Brazil

Jefferson Aparecido Dias, Eduardo Federighi Baisi Chagas, Cláudia Rucco Penteado Detregiachi, Fernanda Mesquita Serva, Piero Biteli, Claudemir Gregório Mendes, Elídia Fabiana de Souza Xavier, Carlos Francisco Bitencourt Jorge, Tereza Lais Menegucci Zutin, Mateus Cezar dos Santos, Daniela Vieira Buchaim, Rogério Leone Buchaim

Subject: Biology And Life Sciences, Virology Keywords: covid-19 antibody; coronavirus; immunoglobulin; police officer; quality of life; SARS-CoV-2

Online: 19 August 2020 (09:55:16 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202008.0316.v1

Next-Generation Multimodality of Nanomedicine Therapy: Folic Acid Conjugated Copolymer and Folate Receptor Interactions Disrupt Receptor Functionality Resulting in Dual Therapeutic Anti-Cancer Potential in Triple-Negative Breast Cancer

Alexandria DeCarlo, Cecile Malardier-Jugroot, Myron R Szewczuk

Subject: Chemistry And Materials Science, Nanotechnology Keywords: nanoparticles; FA-DABA-SMA; drug delivery systems; folic acid receptor alpha; intracellular disruptions; invasion and migration; breast cancer

Online: 14 August 2020 (09:49:17 CEST)

Show abstract| Download PDF| Share

The development of a highly specific drug delivery system (DDS) for anti-cancer therapeutics is an area of intense research focus. Chemical engineering of a “smart” DDS to specifically target tumor cells has gained interest, designed for safer, more efficient, and effective use of chemotherapeutics for the treatment of cancer. However, the selective targeting and choosing the critical cancer surface biomarker are essential for targeted treatments to work. The folic acid receptor alpha (FRalpha) has gained popularity as a potential target in triple-negative breast cancer (TNBC). We have previously reported on a functionalized folic acid (FA)-conjugated amphiphilic alternating copolymer poly(styrene-alt-maleic anhydride) (FA-DABA-SMA) via a biodegradable linker 2,4-diaminobutyric acid (DABA) that has the essential features for efficient “smart” DDS. This biocompatible DDS self-assembles in a pH-dependent manner, providing stimuli-responsive, active targeting, extended-release of hydrophobic chemotherapeutic agents, and can effectively penetrate the inner core of 3-dimensional cancer spheroid models. The empty FA-DABA-SMA decreased spheroid volume, revealing a previously unknown mechanism of action. Upon further investigation, a size- and shape-dependent interaction FA-DABA-SMA with FR reduced the expression of p53, the product of the highly mutated TP53 gene, and additional oncogenic c-Myc and STAT3 proteins. Here, we investigated how this copolymer influences FR behavior and disrupting the receptor’s functions. Results indicate that FA-DABA-SMA increases FR expression levels in breast MDA MB-231 cancer cells and disrupting FR signaling by the reduction in HES1 and NOTCH1 protein expression levels. Also, FA-DABA-SMA induces apoptosis and further causes a change in the morphology of the MDA MB-231 cells, as well as significantly reduces their ability to migrate in a Scratch wound assay. Collectively, these findings provide a novel insight into the functionalized FA-DABA-SMA copolymer. The 350 kDa and 20 kDa copolymers actively target FRα to initialize internationalization. However, only the large size and sheet-shaped 350 kDa copolymers disrupt FRα signaling. The significance of these novel findings reveals the intracellular activity of the copolymer that is critically dependent on the size and structural shape. This report offers novel therapeutic insight into a dual mechanism of FA-DABA-SMA copolymer for its therapeutic potential for the treatment of cancer.

Preprint ARTICLE | doi:10.20944/preprints202008.0307.v1

Bringing onco-innovation to Europe’s healthcare systems: the unexploited potential of biomarker testing, real world evidence and the potential of Tumour Agnostics. The lesson from BRCA1/2 genetic testing

Denis Horgan, Gennaro CILIBERTO, Pierfranco Conte, Giuseppe CURIGLIANO, Luis Seijo, Luis M. Montuenga, Luis Paz-Ares, Marina Chiara Garassino, Penault-Llorca Frederique, Fabrizia GALLI, Isabelle Ray-Coquard, Denis Querleu, David R. Baldwin, Susana Banerjee, Peter H.J. Riegman, Keith Kerr, Benjamin Horbach, Reinhard Büttner, Hendrik Van Poppel, Anders Bjartell, Giovanni Codacci-Pisanelli, Westphalen Christoph Benedikt, Fabien CALVO, Jasmina Koeva-Balabanova, Stephen Hall, Angelo Virgilio Paradiso, Dipak. Kalra, Christa M. Cobbaert, Rocio Varea Mendez, Zorana Maravic, Vassiliki Fotaki, Jaafar Bennouna, Estelle Cauchin, Núria Malats, Iñaki Gutiérrez-Ibarluzea, Benjamin Gannon, Ken Mastris, Chiara Benini, William M. Gallagher, Simonetta Buglioni, Alastair Kent, Elisabetta Munzone, Ivica Belina, Jan P. van Meerbeeck, Michael Joe Duffy, Elzbieta. Sarnowska, Beata Jagielska, Sarah Mee, Ettore Domenico Capoluongo

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: cancer biomarkers, BRCA1/2, genomics; European recommendation

Online: 14 August 2020 (04:27:45 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202003.0352.v1

Maternal Subpopulation Variances in Vaginal and Cesarean Section Delivery Method Predicts Excess Infant Mortality of Black/African Americans in the United States: Linked Birth/Infant Death Records, 2007-2016

Laurens Holmes. Jr, Leah O’Neill, Hikma Elmi, Camillia Comeaux, Chinaka Chinacherem, Lavisha Pelaez, Kirk W. Dabney, Olumuyiwa Akinola, Michael Enwere

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: vaginal; cesarean section; African American women; infant mortality; race/ethnicity

Online: 23 March 2020 (11:21:46 CET)

Show abstract| Download PDF| Share

Objective: Racial/ethnic disparities in infant mortality (IM) continue to persist in the United States, with Black/African Americans (AA) being disproportionally affected with threefold increase in mortality compared to Whites. Epidemiologic data have identified maternal characteristics as risk IM such as eclampsia, maternal education, smoking, maternal weight, maternal SES, and family structure. Understanding the cause of causes including the method of labor and delivery and the racial heterogeneity may facilitate intervention mapping in narrowing the Black White IM risk differences. We aimed to assess the temporal/racial trends and the methods of delivery, mainly vaginal versus cesarean section (C-section) as exposure function of IM. Methods: The United States linked Birth/Infant Death records (2007-2016) were used with a cross-sectional ecologic design. The analysis involved chi squared statistic, incidence rate estimation, and period percent change. Results: Of the 40,445,070 births between 2007 and 2016, cumulative mortality incidence was 249,135 (1.16 per 1000). The IM rate was highest among Black/AA (11.41 per 1000), intermediate among Whites (5.19 per 1000), and lowest among Asian /Pacific Islanders (4.24 per 1000). The cumulative incidence rate difference, comparing vaginal to cesarean procedure was 1.73 per 1000 infants, implying excess IM with C-section. Compared to C-section, there was a 31% decreased risk of IM among mothers with vaginal delivery, rate ratio (RR) = 0.69, 95% CI 0.64-0.74. Racial disparities was observed in the method of delivery associated with IM. Black/AA mothers with vaginal delivery had a 6% decreased risk of IM compared to C-section, RR = 0.94, 95%CI 0.92-0.95, while Whites with vaginal delivery had a 38% decrease risk of IM relative to C-section, RR= 0.68, 95%CI 0.67-0.69, p<0.001. Conclusion: Infant mortality varied by race, with Black/AA disproportionally affected which is explained in part by labor and delivery procedures, suggesting reliable and equitable intrapartum assessment of Black/AA mothers during labor.

Working Paper ARTICLE

Lie Group Statistics and Lie Group Machine Learning Based on Souriau Lie Groups Thermodynamics & Koszul-Souriau-Fisher Metric: New Entropy Definition as Generalized Casimir Invariant Function in Coadjoint Representation

Frédéric Barbaresco

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Lie Groups Thermodynamics; Lie Group Machine Learning; Kirillov Representation Theory; Coadjoint Orbits, Moment Map; Covariant Gibbs Density; Maximum Entropy Density; Souriau-Fisher Metric; Generalized Casimir Invariant Function

Online: 6 March 2020 (02:49:26 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202003.0098.v1

Using ISSR Genomic Fingerprinting to Study the Genetic Differentiation of Artemia Leach, 1819 (Crustacea: Anostraca) from Iran and Neighbor Regions with the Focus on the Invasive American Artemia franciscana

Amin Eimanifar, Alireza Asem, Pei-Zheng Wang, Weidong Li, Michael Wink

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: genetic variation; brine shrimp Artemia; invasive species; mt-DNA COI; Inter-Simple Sequence Repeats (ISSRs) genomic fingerprinting; Western Asia

Online: 6 March 2020 (02:43:06 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202003.0063.v1

Effects of in ovo Supplementation with Nano-nutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

Sivakumar Subramaniyan, Kang Darae, Sharif Hasan Siddiqui, Jin Ryong Park, Weishun Tian, Yong Park Byung, Kwan Seob Shim

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: nanoparticles; silver; L-arginine; conjugation; muscle growth

Online: 4 March 2020 (10:45:11 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201912.0379.v1

Post-Transplant Lymphoproliferative Diseases in the Elderly after Cardiac Transplantation: The Diagnostic Role of [18f] FDG-PET With Co-Registered CT and the Effect of Sports Activity Rehabilitation Program

Antonello Sica, Maria Luisa de Rimini, Beniamino Casale, Caterina Sagnelli, Alessandro Spada, Alfonso Reginelli, Armando Calogero, Cristiano Amarelli, Ciro Maiello, Evangelista Sagnelli, Maria Paola Belfiore, Salvatore Cappabianca

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: post-transplant lymphoproliferative disorder; FDG-PET/CT; elder age; physical activity

Online: 29 December 2019 (10:37:20 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Effect of Cross-Linking Degree of EPDM Phase on the Morphology Evolution and Crystallization Behavior of Thermoplastic Vulcanizates Based on Polyamide 6 (PA6)/Ethylene-Propylene-Diene Rubber (EPDM) Blends

Lifeng Ma, Wei Yang, Hui Guo

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: thermoplastic vulcanizates (TPVs); dynamic vulcanization; morphology evolution; crystallization behavior

Online: 24 July 2019 (12:10:36 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201907.0140.v1

Pathway-Focused Gene Interaction Analysis Reveals the Regulation of TGFβ, Pentose Phosphate and Antioxidant Defense System by Placental Growth Factor in Retinal Endothelial Cell Functions: Implication in Diabetic Retinopathy

Hu Huang, Madhu Sudhana Saddala, Anton Lennikov, Anthony Mukwaya, Lijuan Fan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: PlGF; PGF; blood-retinal barrier; RNA Seq; HREC; gene ontology; fastQC; Trimmomatic; KEGG; pentose phosphate pathway; TGF-β

Online: 10 July 2019 (07:48:20 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201905.0381.v1

Influence of Pore Networking and Electric Current Density on the Crack Pattern in Reinforced Concrete Test Due to Pressure Rust Layer at Early Ages of an Accelerated Corrosion Test

Ángela M. Bazán, Encarnación Reyes, Jaime C. Gálvez

Subject: Engineering, Civil Engineering Keywords: corrosion; concrete cover; cracking; SEM image analysis; rust layer; strain gauge; pore size

Online: 31 May 2019 (08:46:01 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201903.0105.v1

Upregulation of the Nitrosylome in Bipolar Disorder Type 1 (BP1), but not BP2, and Major Depression: Increased IgM Antibodies to Nitrosylated Conjugates are Associated with Indicants of Leaky Gut

Michael Maes, Denitsa Simeonova, Drozdstoy Stoyanov, Jean–Claude Leunis

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: depression, bipolar disorder, oxidative and nitrosative stress, neuro-immune, inflammation, cytokines

Online: 8 March 2019 (09:13:40 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201902.0077.v1

Understanding the Double-Level Influence of Guanxi on Construction Innovation in China: The Mediating Role of Interpersonal Knowledge Sharing and the Cross-level Moderating Role of Inter-Organizational Relationships

Yingmiao Qian, Mengjun Wang, Yang Zou, Ruoyu Jin, Ruijia Yuan

Subject: Engineering, Civil Engineering Keywords: inter-personal relationships; construction innovation; knowledge sharing; inter-organizational relationships

Online: 8 February 2019 (09:20:06 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201808.0496.v2

Synopsis of a New Taxonomic Synthesis of Montiaceae (Portulacineae) Based on Rational Metadata Analysis, with Critical New Insights on Historically Poorly Understood Taxa and a Reevaluation of Historical Biogeography

Mark Hershkovitz

Subject: Biology And Life Sciences, Plant Sciences Keywords: Montiaceae, taxonomy, phylogeny, biogeography, evolution, Calandrinia, Cistanthe, Lewisiopsis, Philippiamra, Rumicastrum

Online: 8 October 2018 (14:02:59 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201807.0509.v1

Effect of Randomisation to 6-Month Mediterranean versus Low-Fat Diet Intervention on Inflammation and Adiposity in Patients with Coronary Heart Disease; Preliminary Results of the AUSMED Heart Trial

Hannah L. Mayr, Catherine Itsiopoulos, Audrey C. Tierney, Teagan Kucianski, Jessica Radcliffe, Colleen J. Thomas

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: inflammation; coronary disease; mediterranean diet; low-fat diet; C-reactive protein; adiponectin; visceral fat; body composition

Online: 26 July 2018 (10:33:01 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201806.0286.v1

Rainfall Prediction with AMSR-E Soil Moisture Products Using SM2RAIN and Nonlinear Autoregressive Networks with Exogenous Input (NARX) for Poorly Gauged Basins: Application to the Karkheh River Basin, Iran

Majid Fereidoon, Manfred Koch

Subject: Engineering, Civil Engineering Keywords: soil moisture; NARX neural networks; AMSR-E; SM2RAIN; Karkheh River Basin

Online: 19 June 2018 (09:32:11 CEST)

Show abstract| Download PDF| Share

Accurate estimates of daily rainfall are essential for understanding and modeling the physical processes involved in the interaction between the land surface and the atmosphere. In this study, daily satellite soil moisture observations from the Advanced Microwave Scanning Radiometer - Earth Observing System (AMSR-E) generated by implementing the standard NASA- algorithm are employed for estimating rainfall, firstly, through the use of recently developed approach, SM2RAIN (Brocca et al., 2013) and, secondly, the nonlinear autoregressive network with exogenous inputs (NARX) neural modelling at five climate stations in the Karkheh river basin (KRB), located in southwest Iran. In the SM2RAIN method, the period 1 January 2003 to 31 December 2005 is used for the calibration of algorithm and the remaining 9 months from 1 January 2006 to 30 September 2006 is used for the validation of the rainfall estimates. In the NARX model, the full study period is split into a training (1 January 2003 to 31 September 2005) and a testing (1 September 2005 to 30 September 2006) stage. For the prediction of the rainfall as the desired target (output), relative soil moisture changes from AMSR-E and measured air temperature time series are chosen as exogenous (external) inputs in NARX. The quality of the estimated rainfall data is evaluated by comparing it with observed rainfall data at the five rain gauges in terms of the correlation coefficient R, the RMSE and the statistical bias. For the SM2RAIN method, R ranges between 0.44 and 0.9 for all stations, whereas for the NARX- model the values are generally slightly lower. Moreover, the values of the bias for each station indicate that although SM2RAIN is likely to underestimate large rainfall intensities, due to the known effect of soil moisture saturation, its biases are somewhat lower than those of NARX. In conclusion, the results of the present study show that with the use of AMSR-E soil moisture products in the physically based SM2RAIN- algorithm as well as in the NARX neural network, rainfall for poorly gauged regions can be fairly predicted.

Preprint ARTICLE | doi:10.20944/preprints201806.0042.v1

Seasonal Growth of Zygophyllum dumosum Boiss.: Summer Dormancy is Associated with Loss of the Permissive Epigenetic Marker Dimethyl H3K4 and Extensive Reduction in Proteins Involved in Basic Cell Functions

Janardan Khadka, Narendra Singh Yadav, Gila Granot, Gideon Grafi

Subject: Biology And Life Sciences, Plant Sciences Keywords: epigenetics; H3K9 methylation; H3K4 methylation; DNA methylation; seasonal climate change; summer dormancy; heat shock proteins; ribosomal proteins; Zygophyllum dumosum Boiss

Online: 4 June 2018 (12:49:59 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201710.0110.v1

Multi Component Reactions under Increased Pressure: On the Mechanism of Formation of Pyridazino[5,4,3-de][1,6]naphthyridine Derivatives from Reaction of Malononitrile, Aldehyde and 2-Oxoglyoxalarylhydrazones in Q-Tubes

Majdah A. AL-Johani, Khadijah M. Al-Zaydi, Sameera M. Mousally, Norah F. Alqahtani, Mohamed H. Elnagdi

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: X-ray; arylhydrazones; 2-amino-1,1,3-propenetricarbonitrile; pyridazines; rate enhance under pressure

Online: 17 October 2017 (04:13:47 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201709.0119.v1

Lp16-PSP, A Member of YjgF/YER057c/UK114 Protein Family Induces Apoptosis and p21WAF1/CIP1 Mediated G1 Cell Cycle Arrest in Human Acute Promyelocytic Leukemia (APL) HL-60 Cells

Thomson Patrick Joseph, Warren Chanda, Abdullah Faqeer Mohammad, Sadia Kanwal, Samana Batool, MeiShan Zhang, MinTao Zhong, Min Huang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: lentinula edodes; Lp16-PSP; acute promyeloid leukemia; extrinsic and lntrinsic apoptotic pathway; G1 phase cell cycle arrest

Online: 25 September 2017 (08:53:12 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201706.0060.v1

In Vitro Quantified Determination of β-Amyloid 42 Peptides, a Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using a Simple, Cost-effective Thin Gold Film Biosensor

Yifan Dai, Alireza Molazemhosseini, Chung Chiun Liu

Subject: Chemistry And Materials Science, Biomaterials Keywords: β-amyloid 42; differential pulse voltammetry; Neuro-degenerative disorders; ferrocyanide/ferricyanide redox couple

Online: 13 June 2017 (18:14:57 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202410.0884.v1

A Modified Novel Validated High-Throughput Hemagglutinin Inhibition Assay Using Recombinant Virus-Like Particles and Human Red Blood Cells for the Objective Evaluation of Recombinant Hemagglutinin Nanoparticle Seasonal Influenza Vaccine

Timothy S. Vincent, Mingzhu Zhu, Anand Parekh, Urvashi Patel, Shane Cloney-Clark, Andrew Klindworth, David Silva, Andrew Gorinson, Karlee Miranda, Mi Wang, Zachary Longacre, Bin Zhou, Iksung Cho, Rongman Cai, Raj Kalkeri, Louis Fries, Vivek Shinde, Joyce S Plested

Subject: Biology And Life Sciences, Virology Keywords: hemagglutination inhibition; immunogenicity; influenza vaccine; influenza virus; singleton; validation; virus-like particles; human red blood cells; egg-derived influenza viruses; CypherOne™ Hemagglutination Analyzer (InDevR)

Online: 11 October 2024 (11:01:34 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1121.v1

Exploring Boscia senegalensis-Derived Boscisucrophage: A Novel Dual SGLT1 and SGLT2 Inhibitor Enhancing Glycemic Control in Oral Drug-Resistant Type 2 Diabetes Patients: Insights from a Preliminary Observational Cohort Study

Bruno Eto, Joe Miantezila Basilua, Ahlam Outman, Rosette Christelle Ndjip, Ali S. Alqahtani, Mohamed Bourhim, Brahim Ibet, Issa Ramadam, Claire Torina, Panatere Pagui, Kady Tchalkoutou, Nour Mahamat, Janine Cordier, Bernard Gressier, Jehan-François Desjeux

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: glycosylated haemoglobin; Oral antihyperglycemic drugs resistant; diabetes type 2; Boscisucrophage; gliflozin; dual SGLT1 and SGLT2 inhibition

Online: 15 August 2024 (06:28:06 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1009.v1

Cepharanthin®, an Alkaloid Mixture Derived from Stephania cephalantha Hayata Suppresses the Activation of T-lymphocytes and the Inflammatory Response of Epithelial Cells in In Vitro Oral Lichen Planus Model

Ryo Shiraishi, Yuske Komiyama, Yoshiaki Kitsukawa, Aya Koike, Toshiki Hyodo, Yosuke Kunitomi, Erika Yaguchi, Tomonori Hasegawa, Wataru Kotani, Rei Wada, Saki Tamegaya, Chonji Fukumoto, Takahiro Wakui, Hitoshi Kawamata

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: oral lichen planus; Stephania cephalantha Hayata; cepharanthine; IL-1β ; NF-κ B

Online: 14 August 2024 (08:20:49 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.1531.v1

Exploring the Impact of Consumer Trust and Perceived Risks on the Intentions to Purchase Overseas Travel Packages via Social Media in Thailand: an Application of the Fuzzy Set Delphi Method

Pongchatorn Kulnadee, Sumaman Pankham

Subject: Business, Economics And Management, Business And Management Keywords: Purchase Intention; Trust; Perceived Risk; Social Media; Fuzzy Set Delphi Method

Online: 23 May 2024 (10:41:03 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202404.1421.v1

An Emerging Concentric Spatial Turn for Sustainable Systems: Beyond the Diametric Spatial Frame in Bacon’s View of Humans as Apart from and Above the Natural World Towards Being-Alongside Nature

Paul Downes

Subject: Social Sciences, Psychology Keywords: nature; othering; hierarchy; concentric space; diametric space; inclusion; descartes; western ethnocentrism

Online: 22 April 2024 (18:15:51 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202404.1296.v1

Absolute Rate Constants for the Reaction of Benzil and 2,2’-Furil Triplet with Substituted Phenols in the Ionic Liquid 1-Butyl-3-methylimidazolium Hexafluorophosphate. A Nanosecond Laser Flash Photolysis Study

José Carlos Netto-Ferreira, Ada Ruth Bertoti

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: benzil; 2,2´-furil; nanosecond laser flash photolysis; triplet excited state; ionic liquids; phenolic hydrogen abstraction

Online: 19 April 2024 (10:55:26 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0977.v1

Analysis of the Medication Patterns of San Yin Si Tian Fang in the “San Yin Ji Yi Zheng Fang Lun” Based on the Ancient and Modern Medical Record Cloud Platform

Shuai Zhang, Hanqing ZHAO

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: Chen Wuxuan; San Yin Si Tian Fang; Prescription pattern; Ancient and Modern Medical Case Cloud Platform

Online: 19 February 2024 (14:50:37 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0880.v1

Combined Use of Fatty Acid Profiles and Elemental Fingerprints to Trace the Geographic Origin of Live Baits for Sports Fishing: The Solitary Tube Worm (Diopatra neapolitana) as a Case Study

Fernando Ricardo, Marta Lobão Lopes, Renato Mamede, M. Rosário Domingues, Eduardo Ferreira da Silva, Carla Patinha, Ricardo Calado

Subject: Biology And Life Sciences, Food Science And Technology Keywords: bait fishing; harvesting; polychaetes; resource management; Ria de Aveiro; sustainability

Online: 16 February 2024 (07:43:46 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0444.v1

Are Tier 2 Metropolises More or Less Affected by Housing Asset Value Deflation under a Depopulation Era? By Comparing the Tokyo Metropolitan Area and the Kansai Metropolitan Area in Japan

Masaaki Uto, Sophie Buhnik, Yuki Okazawa

Subject: Social Sciences, Geography, Planning And Development Keywords: asset values; depopulation; land prices; metropolises; shrinking cities; regression model

Online: 7 February 2024 (12:52:01 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0190.v1

Effect of a Gluten Free Diet in Whole Gut Transit Time in Celiac Disease (CD) and Non-gluten Celiac Sensitivity (NGCS) Patients. A Study Using the Wireless Motility Capsule (WMC)

Orestes Cobos-Quevedo, Gildardo Alfonso Hernandez, Xaira Jimena Rivera-Gutiérrez, Peter Grube-Pagola, Jose Maria Remes-Troche

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: celiac disease; gluten; diet; non-celiac sensitivity; wheat

Online: 5 February 2024 (06:59:53 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.2195.v1

The Influence of High Dose Parenteral Vitamin C Supplementation on Incidence and Severity of Postoperative Pulmonary Complications after Cardiac Surgery with Extracorporeal Circulation: A Prospective, Randomized, Single-Blinded, Interventional Study

Milica Karadžić Kočica, Arsen Ristić, Ivan Soldatović, Dejan Lazović, Jelena Čumić, Miloš Grujić, Radmila Karan, Duško Terzić, Ivan Palibrk, Mladen Kocica, Dejan Marković

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: vitamin C; ascorbic acid; high dose; parenteral administration; cardiac surgery; extracorporeal circulation; postoperative pulmonary complications

Online: 31 January 2024 (08:56:46 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202401.2063.v1

In Vivo and In Vitro Evaluation of the Antibacterial Activity of the Synthetic Peptide LyeTx I mn∆K, Derived From Lycosa erythrognatha Spider Toxin, Against Methicillin-Resistant Staphylococcus aureus (MRSA)

Ana Paula Gonçalves Coelho Vieira, Amanda Neves De Souza, William Gustavo Lima, Julio Cesar Moreira Brito, Daniela Carolina Simião, Lucas Vinicius Ribeiro Gonçalves, Lídia Pereira Barbosa Cordeiro, Denise de Oliveira Scoaris, Simone Odília Antunes Fernandes, Jarbas Magalhães Resende, Burkhard Bechinger, Rodrigo Moreira Verly, Maria Elena De Lima

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Antimicrobial peptides; Methicillin-resistant Staphylococcus aureus (MRSA); Lycosa erythrognatha; Skin infections; Action on membrane.

Online: 30 January 2024 (05:00:30 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.1927.v1

Changes in Social Media Big Data on Healing Forests : A Time-series Analysis on the Use Behavior of Healing Forests Before and After the COVID-19 Pandemic in South Korea

Sangwook Kim, Juyeong Youn

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: healing forest; COVID-19; text mining; network analysis; quadratic assignment; procedure correlation analysis

Online: 26 January 2024 (13:41:21 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1861.v1

Expressed in HPV 16 Infected Epidermoid Carcinoma Cells, HIV‐1 Reverse Transcriptase Shifts Their Metabolism towards aerobic Glycolysis, Induces Hybrid E/M Cell Phenotype and Increases Expression of E6 Transcripts

Alla S Zhitkevich, Ekaterina O Bayurova, Daria V Avdoshina, Natalia F Zakirova, Galina A Frolova, Juris Jansons, Sona Chowdhury, Alexander V Ivanov, Ilya V Gordeychuk, Joel M. Palefsky, Maria G Isaguliants

Subject: Biology And Life Sciences, Virology Keywords: HIV-1; reverse transcriptase; HPV16-positive cells; E6*I isoform expression; glycolysis; mitochondrial respiration

Online: 25 December 2023 (13:18:26 CET)

Show abstract| Download PDF| Supplementary Files| Share

High incidence of epithelial malignancies in HIV-1 infection is associated with co-infection with oncogenic viruses, such as high-risk human papillomaviruses (HR HPVs), most often HPV16. A collaboration between HIV-1 and HR HPVs in the malignant transformation of epithelial cells exposed to both viruses have long been anticipated. Here, we delineated the effects on in vitro and in vivo properties of HPV16-infected cervical cancer cells of HIV-1 reverse transcriptase. Epidermoid carcinoma Ca Ski cells were made to express RT of HIV-1 clade A (RT_A) or Green Fluorescence Protein (GFP) by lentiviral transduction, subclones with one or six copies of RT_A and six copies of GFP DNA were generated. Expression by subclones of GFP was assessed by flow cytometry, and of RT_A, by Western blotting. Cells were assessed for the levels of mRNA of the RT_A, E6 and its isoforms, and cellular factors characterizing oxidative stress, and EMT by the real-time PCR. Parameters of glycolysis and mitochondrial respiration were assessed by Seahorse technology. Subclones were surveyed for the changes in cell cycle, doubling time, migration capacity, clonogenic activity and for the capacity to form tumors in nude mice. Ca Ski RT_A produced 20-55 fg of RT_A per cell. Expression of RT_A caused a proportional increase in the expression of E6*I isoform. In Ca Ski, RT_A suppressed mitochondrial respiration and oxygen consumption, and increased glycolysis. Compared to parental cells and GFP control, transcription in Ca Ski RT_A of epithelial (E-CADHERIN) state marker was increased, and of mesenchymal (VIMENTIN) decreased, indicating acquisition by cells of the hybrid epithelial/mesenchymal (E/M) phenotype. While Ca Ski GFP and Ca Ski RT_A with one gene insert had reduced migration rate, decreased clonogenic activity in vitro, and diminished capacity to form tumors in nude mice, increase in RT_A copicity/expression mitigated these effects. Altogether, expression of HIV-1 RT_A gave Ca Ski cells the plasticity required to overcome negative effects of lentiviral transduction and potentially increased their tumorigenicity.

Preprint ARTICLE | doi:10.20944/preprints202311.1997.v1

People-Oriented: A Framework for Evaluating the Level of Green Space Provision in the Life Circle from a Supply and Demand Perspective: A Case Study of Gulou District, Nanjing, China

Hancheng Xia, Yin Rui, Tianyu Xia, Bing Zhao, Bing Qiu

Subject: Social Sciences, Urban Studies And Planning Keywords: green space; community life circle; provision level; resident demand; assessment framework; population model

Online: 30 November 2023 (14:18:53 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1848.v1

Cbct Evaluation of the Relationship between the Distance of the Apex of the Upper Central Tooth to the Nasal Floor and the Anterior Nasal Spine with Orthodontic Vertical Direction Parameters

Saadet Çınarsoy Ciğerim, Türkan Sezen Erhamza

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: orthodontic treatment; upper central; anterior nasal spine; nasal floor; CBCT; vertical growth pattern

Online: 29 November 2023 (06:31:31 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202310.1821.v1

Etiopathogenesis of Type 2 Diabetes in Patients from the Asir Region of Saudi Arabia: Association of Pro-inflammatory Cytokines CCL-1, 2, 4, and 5 with Varying Levels of Obesity

Mohammad Muzaffar Mir, Jaber Alfaifi, Shahzada Khalid Sohail, Syeda Fatima Rizvi, Mushabab A. Alghamdi, Rashid Mir, Javed Iqbal Wani, Zia UL Sabah, Fahad A. Alhumaydhi, Fahad Nasser Alremthi, AbdulElah Al Jarallah AlQahtani, Muffarah Hamid Alharthi, Masoud Ishag, Imadeldin Elfaki, Hany M. A. Sonpol

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: type-2 diabetes mellitus; pro-inflammatory chemokines; obesity; CCL1; CCL2; CCL4; CCL5

Online: 27 October 2023 (15:52:34 CEST)

Show abstract| Download PDF| Share

Background. Type 2 diabetes mellitus (T2DM) is becoming a major global health concern, especially in poorer nations. The high prevalence of obesity and the ensuing diabetes is attributed to rapid economic progress, physical inactivity, consumption of high-calorie foods, and changing lifestyles. Objectives. We investigated the role of pro-inflammatory chemokines; CCL1, 2, 4 and 5 with varying levels of obesity in T2DM in the Asir region of Saudi Arabia. Materials and methods. 170 confirmed T2DM patients and a normal control group were enrolled. The demographic data, serum levels CCL-1, 2, 4 and 5 and the biochemical indices were assessed in patients and control groups by standard procedures. Results. T2DM patients were divided into four groups: A (normal body weight), B (Overweight), C (obese), and D (highly obese). n controls. We observed that male and female control patients had similar mean serum concentrations of pro-inflammatory chemokines CCL 1, 2, 4, and 5. Chemokines CCL1, CCL2, and CCL4 in the serum of Type 2 Diabetes Mellitus patients with normal or overweight body weights were significantly higher than the control group, regardless of gender. In T2DM individuals with obesity and severe obesity, the rise was most significant. There was a progressive rise in the concentrations of CCL1, 2, and 4 in T2DM) patients with increasing BMI. Serum CCL5 levels increased significantly in all T2DM patient groups. The increase in CCL5 was more predominant in normal-weight people as compared to overweight and obese T2DM patients. Conclusions. Male and female control patients had similar serum levels of pro-inflammatory chemokines CCL 1, 2, 4, and 5. The progressive rise in blood concentrations of three Pro-inflammatory chemokines; CCL1, 2, and 4 in T2DM patients with increasing BMI supports the idea that dyslipidemia and obesity contribute to chronic inflammation and insulin resistance. Serum CCL5 levels increased significantly in all T2DM patient groups. The selective and more pronounced increase of CCL5 in the T2DM group with normal BMI as compared to patients with varying degrees of obesity, was rather surprising. Further research is needed to determine if CCL5 under-expression in overweight and obese T2DM patients is due to some unexplained counterbalancing processes.

Preprint ARTICLE | doi:10.20944/preprints202310.0872.v1

Literature Review on a Simple Method to Achieve Savings in Single-Phase Active Energy in Electric and Synchronous Ventilation Motors – PMSM Type – Greater Than That Obtained With the “Fan Law”

Ibar Anderson

Subject: Engineering, Electrical And Electronic Engineering Keywords: energy efficiency; permanent magnet synchronous motor (PMSM); energy savings; single-phase active AC energy; kWh, Fan law

Online: 18 October 2023 (04:31:43 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202309.1264.v1

Comparison of the Diagnostic Value of Mono-Exponential, Bi-Exponential, and Stretched Exponential Diffusion-Weighted MRI to Predict Hepatic Lymph Node Metastases in Patients with Colorectal Liver Metastases after Chemotherapy

Hai-Bin Zhu, Bo Zhao, Xiao-Ting Li, Xiao-Yan Zhang, Qian Yao, Ying-Shi Sun

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: colorectal cancer; individualized treatment; diffusion magnetic resonance imaging; intravoxel incoherent motion; liver

Online: 19 September 2023 (11:53:35 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202309.1211.v1

Persistence of SARS-CoV-2 IgG and Its Avidity Compared with Neutralizing Antibodies among Health Care Workers in a Tertiary Care Hospital, South India – a One Year Follow Up Study

Anagoni Srikar, Mudhigeti Nagaraja, Alladi Mohan, Pottekula Prakash, Kodavala Sireesha, Nallapireddy Umapathi, Anju Verma, Usha Kalawat

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: SARS-COV-2; Health care workers; IgG antibody; Neutralizing antibody; Antibody avidity

Online: 19 September 2023 (03:35:40 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202308.0151.v1

Dynamics of Anti-SARS-CoV-2 Antibodies in the Albanian Population: Impact of Infection and Vaccine-Induced Immunity during the COVID-19 Pandemic Year from August 2021 to August 2022

Genc Sulcebe, Alban Ylli, Fabian Cenko, Margarita Kurti-Prifti, Erkena Shyti, Jonida Dashi-Pasholli, Erina Lazri, Irena Seferi-Qendro, Melissa J. Perry

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2 Seroprevalence; Vaccination Status; SARS-CoV-2; anti-SARS-CoV-2 antibodies; Albanian population

Online: 2 August 2023 (10:10:13 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202308.0143.v1

How to study the location and size of rectal tumors that are candidates for local surgery: Rigid rectoscopy, Magnetic resonance imaging, En-dorectal Utrasound or colonoscopy? An inter-observational study

Anna Serracant, Eva Ballesteros, Noemi Montes, Marta Sola, Francesc Novell, Beatriz Consola, Xavier Serra-Aracil

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Rectal cancer Rectal Magnetic Resonance Imaging (MRI), transanal endoscopic surgery (TES), rectal ultrasound (EUS), intraoperative rigid rectoscopy (IRR),

Online: 2 August 2023 (07:51:41 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202307.2036.v1

Evaluation of Incidence of Respiratory Syncytial Virus, SARS-CoV-2, Influenza A/B, and Adenovirus Cases via Rapid Tests in Community during the Winter 2023 Season. A Cross Sectional Study

Dimitrios Papagiannis, Garyfallia Perlepe, Theodora Tendolouri, Polyxeni Karakitsiou, Georgia Damagka, Anna Kalaitzi, Sofia Alevra, Foteini Malli, Konstantinos I. Gourgoulianis

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: RSV; SARS-CoV-2; Influenza A/B; adenovirus; rapid test; Vaccinations

Online: 31 July 2023 (04:17:26 CEST)

Show abstract| Download PDF| Share

Respiratory infections constitute a major reason for infants and children seeking medical advice and visiting health facilities, thus remaining a significant public threat with high morbidity and mortality. The predominant viruses causing viral respiratory infections are influenza A and B viruses (Flu-A, Flu-B), Respiratory Syncytial Virus (RSV), Adenovirus and Coronaviruses. We aimed to record the incidence of RSV, SARS-CoV-2, Influenza A/B and Adenovirus cases with rapid antigen tests and validate the results with RT-PCR assay of upper respiratory specimens with a wide range of viral loads and (co)-infection patterns in children. Clinical samples were collected from early symptomatic children (presenting with fever and/or cough and/or headache within 5–7 days). The surveillance program was conducted in five private pediatrician dispensaries and one Pediatric care unit, from 10 January 2023 to 30 March 2023 in central Greece. The total sample of specimens collected was 784 young children and infants, of which 383 (48.8%) ware female and 401 were male (51.2%). The mean age of participants was 7.3 + 5.5 years. The sensitivity of FLU A & B test was 91.15% (95% CI: 84.33% -95.67%), and the specificity was 98.96% (95% CI: 97.86% - 99.58%). The sensitivity and specificity of the adenovirus and RSV test was {92.45% (95% CI: 81.79% - 97.91%), 99.32% (95% CI: 98.41% -99.78%)} and {92.59% (95% CI: 75.71% -99.09%), 99.47% (95% CI: 98.65% - 99.86%)} respectively. Lastly, the sensitivity of the SARS-CoV-2 test was 100.00% (95% CI: 79.41%-100.00%) and the specificity was 99.74% (95% CI: 99.06% - 99.97%). We recorded proportion of incidence for the Influenza-A and B, (14.3%), - (3.44%) correspondingly, followed by the adenovirus with a proportion (6.9%) and the RSV with (3.7%) incidence proportion and finally, the SARS-CoV-2 (2.3%). The combination of a new multiple rapid test with multiple antigens, will be probably a useful tool with financial impact for health systems targeting the early detection and right treatment of respiratory infections in the emergency departments in primary health care facilities.

Preprint ARTICLE | doi:10.20944/preprints202307.0974.v1

The Correlation between Methylation Disorders Caused by the MTHFR Polymorphism and the Folates (Folic Acid, 5-MTHF) Metabolism, in Coronary Artery Disease Patients Diagnosed by Invasive Coronary Angiography—Preliminary Results

Agnieszka Pietruszyńska-Reszetarska, Robert Pietruszyński, Ireneusz Majsterek, Tomasz Popławski, Maciej Skrzypek, Beata Kolesińska, Joanna Waśko, Cezary Watała, Joanna Kapusta, Robert Irzmański

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: methylation, 5-MTHF, folic acid, gene polymorphism, coronary artery disease, biomarker

Online: 14 July 2023 (09:46:28 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202306.0119.v1

Quantifying the Potential Contribution of Submerged Aquatic Vegetation to Coastal Carbon Capture in a Delta System from Field and Landsat 8/9-OLI data with Deep Convolutional Neu-ral Network

Bingqing Liu, Tom Sevick, Hoonshin Jung, Erin Kiskaddon, Tim Carruthers

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Submerged aquatic vegetation; Carbon balance model; Landsat 8/9-OLI; Deep learning

Online: 2 June 2023 (03:42:30 CEST)

Show abstract| Download PDF| Supplementary Files| Share

: Submerged aquatic vegetation (SAV) are highly efficient at carbon sequestration and, despite their relatively small distribution globally, are recognized as a potentially valuable component of climate change mitigation. However, SAV mapping in tidal marshes presents a challenge due to optically complex constituents in the water. The emergence and advancement of deep learn-ing-based techniques in the field of habitat mapping with remote sensing imagery provides an opportunity to address this challenge. In this study, an analytical framework was developed to quantify the carbon sequestration of SAV habitats in the Atchafalaya River Delta Estuary from field and remote sensing observations using deep convolutional neural network (DCNN) tech-niques. A U-Net based model, Wetland-SAV Network, was trained to identify SAV percent cover (high, medium, and low) as well as other estuarine habitat types from Landsat 8/9-OLI data. The areal extent of SAV was up to 8% of the total area (47,000 ha) with a significant loss of SAV habitats observed post-Hurricane Barry (~2,300 ha) in 2019. The habitat areas and habitat-specific carbon fluxes were then used to quantify net greenhouse gas (GHG) flux of the study area for with/without SAV scenarios in a Carbon Balance Model. The total net GHG flux was in the range of -0.13 ± 0.06 to -0.86 ± 0.37 ×105 tonne CO2e yr-1 and increased up to 40% (-0.23 ± 0.10 to -0.90 ± 0.39 ×105 tonne CO2e yr-1 ) when SAV was accounted for within the calculation. At the hectare scale, inclusion of SAV resulted in an increase of ~60% for net GHG sink in shallow areas adjacent to emergent marsh where SAV was abundant. This is the first attempt at remotely mapping SAV in coastal Louisiana as well as a first quantification of net GHG flux at the scale of hectares to thousands of hectares, accounting for SAV within these sub-tropical coastal delta marshes. Remote sensing and deep learning models have high potential for mapping and monitoring of SAV in turbid sub-tropical coastal deltas as a component of increasing accuracy of net GHG flux estimates at small (hectare) and large (coastal basin) scales.

Preprint ARTICLE | doi:10.20944/preprints202304.1270.v1

Effect of Meloxicam in a Protocol of Balanced Anaesthesia with Propofol and Sevoflurane on the α- δ- γ-Tocopherols in Response to Inflammatory Oxidative Stress Induced by Ovariectomy in Dogs

Giovanna Lucrezia Costa, Simona Di Pietro, Vincenzo Nava, Patrizia Licata, Nicola Maria Iannelli, Fabio Leonardi, Francesco Macrì, Diego Iannelli, Federica De Paoli, Daniele Macrì, Vincenzo Ferrantelli, Vito Angileri, Giuseppe Bruschetta, Claudia Interlandi

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: Analgesia; Dog; Meloxicam; Oxidative stress; Ovariectomy; Surgery; Tocopherols

Online: 30 April 2023 (09:15:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202304.0406.v1

Dietary Tyrosine Intake Is Associated with Locus Coeruleus Integrity, Attentive Cognitive Control and Grey Matter Maintenance: An MRI Structural Study on 398 Healthy Individuals of the Berlin Aging Study-II

Emanuele Raffaele Giuliano Plini, Michael Christopher Melnychuk, Andrew Harkin, Martin J Dahl, Matthew McAuslam, Simone Kühn, Rory Thomas Boyle, Robert Whelan, Ralph Andrews, Sandra Duzel, Johanna Drewelies, Gert G Wagner, Ulman Lindenberger, Kristina Norman, Ian Hamilton Robertson, Paul Michael Dockree

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: tyrosine; Locus Coeruleus; protein intake; attention; grey matter; neuroimaging; diet; healthy aging

Online: 17 April 2023 (05:00:06 CEST)

Show abstract| Download PDF| Share

Background & aims It is documented that low protein and amino-acid dietary intake is related to poorer cognitive health and increased risk of dementia. Degradation of the neuromodulatory pathways, (comprising the cholinergic, dopaminergic, serotoninergic and noradrenergic systems) is observed in neurodegenerative diseases and impairs the proper biosynthesis of key neuromodulators from micro-nutrients and amino acids. How these micro-nutrients are linked to neuromodulatory pathways in healthy adults is less studied. The Locus Coeruleus–Noradrenergic System (LC-NA) is the earliest subcortical structure affected in Alzheimer’s disease, showing marked neurodegeneration, but is also sensitive for age-related changes. The LC-NA system is critical for supporting attention and cognitive control, functions that are enhanced both by tyrosine administration and chronic tyrosine intake. The purpose of this study was to 1) investigate whether the dietary intake of tyrosine, the key precursor for noradrenaline (NA), is related to LC integrity 2) whether LC integrity mediates the reported association between tyrosine intake and higher cognitive performance (measured with Trail Making Test – TMT), and 3) whether LC integrity relates to an objective measure of brain maintenance (BrainPAD). Methods The analyses included 398 3T MRIs of healthy participants from the Berlin Aging Study II to investigate the relationship between LC integrity and habitual dietary tyrosine intake-daily average (HD-Tyr-IDA). As a control procedure, the same analyses were repeated on other main seeds of the neuromdulatory subcortical system (Dorsal and Medial Raphe, Ventral Tegmental Area and Nucleus Basalis of Meynert). In the same way, the relationships between the five nuclei and BrainPAD were tested. Results Results show that HD-Tyr-IDA is positively associated with LC integrity. Similarly, LC integrity disproportionally relates to better brain maintenance (BrainPAD). Mediation analyses reveal that only LC, relative to the other nuclei tested, mediates the relationship between HD-Tyr-IDA I and performance in the TMT and between HD-Tyr-IDA and BrainPAD. Conclusions These findings provide the first evidence linking tyrosine intake with LC-NA system integrity and its correlation with neuropsychological performance. This study strengthens the role of diet for maintaining brain and cognitive health and supports the noradrenergic theory of cognitive reserve. Within this framework, adequate tyrosine intake might increase the resilience of LC-NA system functioning, by preventing degeneration and supporting noradrenergic metabolism required for LC function and neuropsychological performance.

Preprint ARTICLE | doi:10.20944/preprints202303.0259.v1

Correlation of Plasmatic Profiles of Inflammatory Cytokines/Chemokines and GFAP with Brain Damage Detected at MRI in Neonates with Hypoxic Ischemic Encephalopathy (HIE) Treated with Therapeutic Hypothermia: A Preliminary Study

Cinzia Auriti, Fiammetta Piersigilli, Vito Mondì, Laura Timelli, Tamara Del Pinto, Giusi Prencipe, Giulia Lucignani, Daniela Longo, Iliana Bersani

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Asphyxia; therapeutic hypothermia; newborns; glial fibrillary acidic protein (GFAP); cytokines; chemokines; neuroinflammation

Online: 14 March 2023 (13:22:48 CET)

Show abstract| Download PDF| Supplementary Files| Share

Background & Objective. Perinatal hypoxia acutely triggers the release of cytokines and chemokines from neurons, astrocytes and microglia. In response to hypoxia-ischemia resting/ramified microglia proliferate and undergo activation, with the production of proinflammatory molecules. The brain damage extension seems to be related with both the severity of hypoxia and the balance between pro and anti-inflammatory host response. Methods: In this preliminary study we compared in 10 neonates with hypoxic ischemic encephalopathy (HIE), during therapeutic hypothermia (TH), plasmatic cytokines and other inflammatory molecules levels: monocytic chemotactic-protein-1 (CCL2/MCP-1), CXCL8, glial fibrillary acidic protein (GFAP), Interferon gamma (IFN y), Interleukin 10 (IL-10), IL-18, IL-6, CCL3, ENOLASE2, Colony Stimulating Factor(GM-CSF), IL-1b, IL-12p70, IL-33, Tumor Necrosis Factor alpha(TNF), collected at four different time points during TH (24 hours, 25-48 hours, 49-72 hours) , and after the rewarming (7-10 days from birth). Five of enrolled babies had pathological brain MRI (“cases”) and 5 had a normal examination (“controls”). Cytokines were measured by Magnetic Luminex Assay (Luminex SV 013-17 (14), instrument Biorad Bio-Plex 100 s.n. LX 10005039304). MRI images were classified according to the Barkovich score. Results: Mean levels of all cytokines and molecules at time T1 were not significantly different in the two groups of patients. Comparing samples paired by day of collection, the greatest differences between cases and controls were found at times T2 and T3, during TH. After rewarming, some molecules showed an upward trend. At T4, levels tended to be close again (with the exception of IL-6, IL10 and IL18). Infants with worse MRI showed plasmatic GFAP levels higher than those with normal MRI, while their IL-18 was lower. Mean levels of CCL3MIP1alpha, GMCSF, IL1BETA overlapped throughout the observation period in both the groups of neonates. Conclusion. In children with worse brain MRI we found high levels of GFAP and of IL-10 at T4, considered respectively an early biomarker of brain damage and a predictor of adverse outcome, according to the literature, and a trend towards low IL-18 levels. We found the greatest, although not significant, difference between molecules levels in cases and controls at time points T2 and T3, during TH. With the rewarming some molecules showed an upward trend. We cannot exclude that the small sample size may prevent significance in the differences from being achieved.

Preprint ARTICLE | doi:10.20944/preprints202303.0028.v1

A Health Impact Assessment of an Ergonomic Measure to Reduce the Risk of Work-Related Low Back Pain, Lumbosacral Radicular Syndrome and Knee Osteoarthritis among Floor Layers in the Netherlands

P. Paul F.M. Kuijer, Henk F. Van der Molen, Steven Visser

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: low back pain; osteoarthritis; knee; prevalence; workplace; exposure; musculoskeletal diseases; risk factors; prevention; construction industry

Online: 2 March 2023 (01:55:05 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202302.0445.v1

Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids

Tatsuo Yanagisawa, Eiko Seki, Hiroaki Tanabe, Yoshifumi Fujii, Kensaku Sakamoto, Shigeyuki Yokoyama

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: non-canonical amino acids; genetic code expansion; crystal structure; tRNA; cell-free protein synthesis

Online: 27 February 2023 (06:27:04 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0314.v1

Analysing the Vulnerability State of The Cultural Heritage by Geomorphological and Geophysical Methods: A Case Study on The Black Sea Coast, The Roman Edifice With Mosaic Site of Constanta, Romania

Andreea Andra-Topârceanu, Mihaela Verga, Mihai Mafteiu, Marius Andra, Marian Marin, Radu Daniel Pintilii, Giampietro Mazza, Donatella Carboni

Subject: Environmental And Earth Sciences, Environmental Science Keywords: cultural heritage; Roman Mosaic; geomorphological hazard; geophysical survey; geomorphological methods; vulnerability zoning

Online: 19 December 2022 (03:40:17 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0173.v1

The Stable Operation Analysis of the DC Converter System into a Microgrid System, Taking into Account the Parameters of the Energy Source and the Own Parameters of the DC Converter

Denis Kotin, Ilya Ivanov

Subject: Engineering, Electrical And Electronic Engineering Keywords: DC Microgrid; DC-DC converter; stability analysis; stability boundary; CPL

Online: 9 December 2022 (07:19:54 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202211.0196.v1

Opportunities for Comprehensive Medication Management (CMM) for Children with Special Healthcare Needs and Medical Complexity (CSHCN-CMC): The Methodology of a Prospective Case Series within a Collaborative Practice Agreement (CPA)

Jena Quinn, Heather Monk Bodenstab, Catherine Kuhn, Richard H Parrish II

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: CSHCN; CMC; care coordination; case series; collaboration; medical complexity; medication management; methodology; pediatrics

Online: 10 November 2022 (10:04:10 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202209.0475.v1

A Retrospective, Observational Study of Catheter-associated Urinary Tract Infection Events Post-implementation of a Novel Urinary Catheter System with Active Drain Line Clearance and Automated Intra-abdominal Pressure Monitoring

Patrick Brockway, David M. Hill, Vanessa Moll, Kelly Stanton, Manu L.N.G. Malbrain, Sai R. Velamuri

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: catheter-associated infection; CAUTI; urinary tract infection; healthcare-associated infection; active drain line clearance; burns; intra-abdominal pressure; measurement

Online: 30 September 2022 (04:13:24 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202109.0405.v1

Identity Evolutions, Models of Using Internet and Bonding Social Capital in Iran (A Step in the Direction of Presenting a Structural Model (Path Model) with Case Study of Kerman City)

Alireza Sanatkhah

Subject: Computer Science And Mathematics, Computer Science Keywords: models of using internet; bonding social capital; social identity

Online: 23 September 2021 (11:58:40 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0643.v1

Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium

Vitaliy Yu. Novikov, Nadezhda V. Shumskaya, Vyacheslav A. Mukhin, Konstantin V. Zolotarev, Anton N. Mikhailov, Valeriya I. Nakhod, Marina V. Mikhailova

Subject: Engineering, Automotive Engineering Keywords: processing waste; Atlantic cod; collagen; red king crab; enzyme preparation; collagen hydrolysate; culture medium

Online: 29 July 2021 (10:25:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202106.0405.v1

Process Analysis of Main Organic Compounds Dissolved in Aqueous Phase by Hydrothermal Processing of açaí (Euterpe Oleraceae, Mart.) Seeds: Influence of Process Temperature, Biomass-to-Water Ratio, and Production Scales

Conceição de Maria Sales da Silva, Douglas Alberto Rocha de Castro, Marcelo Costa Santos, Helio da Silva Almeida, Ulf Lüder, Maja Schultze, Judi A Libra, Jan Mumme, Thomas Hoffmann, Nélio Teixeira Teixeira Machado

Subject: Engineering, Automotive Engineering Keywords: Açaí seeds; hydrothermal carbonization; hot compressed water; process analysis, HMF, Furfural, Acetic Acid, Mass Production.

Online: 15 June 2021 (12:08:40 CEST)

Show abstract| Download PDF| Share

This work aims to investigate systematically the influence of process temperature, bio-mass-to-water ratio, and production scales (laboratory and pilot) on the chemical composition of aqueous and gaseous phases and mass production of chemical by hydrothermal processing of Açaí (Euterpe Oleraceae, Mart.) seeds. The hydrothermal carbonization carried out at 175, 200, 225, and 250 °C, 2 °C/min, biomass-to-water ratio of 1:10, and at 250 °C, 2 °C/min, and biomass-to-water ratios of 1:10, 1:15, and 1:20, in technical scale, as well as at 200, 225, and 250 °C, 2 °C/min, bio-mass-to-water ratio of 1:10, in laboratory scale. The elemental composition (C, H, N, S) of solid phase determined to compute the HHV. The chemical composition of aqueous phase determined by GC and HPLC and the volumetric composition of gaseous phase by using an infrared gas an-alyzer. For the experiments in pilot scale with constant biomass-to-water ratio of 1:10, the yields of solid, liquid, and gaseous phases varied between 53.39 and 37.01% (wt.), 46.61 and 59.19% (wt.), and 0.00 and 3.80% (wt.), respectively. The yield of solids shows a smooth exponential de-cay with temperature, while that of liquid and gaseous phases a smooth growth. By varying the biomass-to-water ratios, the yields of solid, liquid, and gaseous reaction products varied be-tween 53.39 and 32.09% (wt.), 46.61 and 67.28% (wt.), and 0.00 and 0.634% (wt.), respectively. The yield of solids decreases exponentially with increasing water-to-biomass ratio and that of liquid phase increases in a sigmoid fashion. For constant biomass-to-water ratio, the concentrations of Furfural and HMF decrease drastically with increasing temperature, reaching a minimum at 250 °C, while that of phenols increases. In addition, the concentrations of CH3COOH and total car-boxylic acids increase, reaching a maximum at 250 °C. For constant process temperature, the concentrations of aromatics vary smoothly with the temperature. The concentrations of furfural, HMF, and cathecol decrease with temperature, while that of phenols increases. The concentra-tions of CH3COOH and total carboxylic acids decrease exponentially with temperature. Finally, for the experiments with varying water-to-biomass ratios, the productions of chemicals (furfu-ral, HMF, phenols, cathecol, and acetic acid) in the aqueous phase is highly dependent on the biomass-to-water ratio. For the experiments in laboratory scale with constant biomass-to-water ratio of 1:10, the yields of solid ranged between 55.9 and 51.1% (wt.), showing not only a linear decay with tem-perature, but also a lower degradation grade. The chemical composition of main organic compounds (furfu-ral, HMF, phenols, cathecol, and acetic acid) dissolved in the aqueous phase in laboratory scale shows the same behavior of those in obtained in pilot scale.

Preprint ARTICLE | doi:10.20944/preprints202103.0272.v1

Examining the Impact of Demographic and Socio-Economic Factors on the Level of Employee Preparedness for a Disaster Caused by Fires: A Case Study of Electrical Power Distribution in Serbia

Vladimir M. Cvetković, Kristina Marković

Subject: Social Sciences, Demography Keywords: disaster; fire; preparedness; respond; employee; demographic; socio-economic; factors

Online: 9 March 2021 (14:49:26 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Effectiveness and Feasibility of Injectable Escherichia coli-Derived Recombinant Human Bone Morphogenetic Protein-2 on Anterior Lumbar Interbody Fusion at Lumbosacral Junction For Adult Spinal Deformity: A Clinical Pilot Study

Sang-Kyu Im, Ki Young Lee, Hae Seong Lim, Seung-Jin Yoo, Jung-Hee Lee

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: adult spinal deformity; lumbosacral junction; anterior lumbar interbody fusion; bone morphogenetic protein; beta-tricalcium phosphate

Online: 28 December 2020 (12:06:09 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Flaviviruses Infections in Neotropical Primates Suggest Long-Term Circulation of Saint Louis Encephalitis and Dengue Virus Spillback in Socioeconomic Regions With High Numbers of Dengue Human Cases in Costa Rica

Andrea Chaves, Martha Piche-Ovares, Eugenia Corrales, Gerardo Suzán, Andrés Moreira-Soto, Gustavo A. Gutiérrez-Espeleta

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Neotropical non-human primates; serology; sylvatic cycles; Flavivirus; Dengue Virus; Saint Louis Encephalitis Virus; West Nile Virus

Online: 5 November 2020 (18:35:56 CET)

Show abstract| Download PDF| Share

Arthropod-borne viruses belonging to the flavivirus genus possess an enormous relevance in public health. Neotropical non-human primates (NPs) have been proposed to be infected more frequently with flaviviruses due to their arboreal and diurnal habits, their genetic similarity to humans and their relative closeness to humans. However, the only known flavivirus in America that is maintained by sylvatic cycles involving NPs is Yellow Fever virus (YFV), and the NPs role as potential hosts of flaviviruses is still unknown. Here, we examined flavivirus exposure in 86 free range and captive NPs of Costa Rica to evaluate their involvement in flavivirus transmission cycles and their potential as flavivirus hosts. We used a highly-specific micro plaque reduction neutralization test (micro-PRNT) to determine the presence of antibodies against YFV, Dengue virus 1-4 (DENV), Zika virus, West Nile Virus (WNV) and Saint Louis Encephalitis virus (SLEV). We found evidence of seropositive NPs to DENV-1 8.2% (homotypic –3/86, heterotypic – 4/86), SLEV 15.1% (homotypic – 10/86, heterotypic – 2/86), WNV 2.3% (homotypic – 2/86) and 8.1% (7/86) undetermined Flavivirus species. No antibodies against YFV or ZIKV were found. This work provides compelling serological evidence of exposure in NPs of flaviviruses associated with urban cycles, i.e. DENV, and confirms decades of circulation of SLEV in the same environments. Also, the range of years of sampling and the socioeconomic region was statistically significant for the presence of Dengue and Flavivirus undetermined seropositive individuals, respectively. Both the years and socioeconomic regions with greater seroprevalence coincide with the years and socioeconomic regions with high numbers of Dengue human cases for the country. Our work suggests bidirectional? circulation of different flaviviruses between humans and wildlife with public health importance and underscores the necessity of further surveillance for flaviviruses in the humans/wildlife interface in Central America.

Working Paper REVIEW

The Alterations of Cellular Signaling Pathways in the Host Cell upon the High Pathogenic Coronaviruses Infection, SARS-CoV and MERS-CoV. What Could Be Expected from the SARS-CoV-2?

Nima Hemmat, Zahra Asadzadeh, Noora Karim-ahangar, Hajar Alemohammad, Basira Najafzadeh, Afshin Derakhshani, Amir Baghbanzadeh, Hossein Bannazadeh Baghi, Darya Javadrashid, Souzan Najafi, Meriadeg Ar Gouilh, Behzad Baradaran

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; coronavirus; signaling pathway; molecular alteration

Online: 21 September 2020 (04:17:24 CEST)

Show abstract| Download PDF| Share

Preprint HYPOTHESIS | doi:10.20944/preprints202006.0253.v1

Is Platelet-Activating Factor (PAF) a Missing Link for Elucidating the Mechanism of Action of the Coronavirus SARS-CoV-2 and Explaining the Side Effects-Complications of COVID-19 Disease?

Constantinos A Demopoulos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Platelet Activating Factor; PAF; SARS-CoV-2; Covid-19

Online: 21 June 2020 (10:27:22 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202005.0335.v1

Influence of Parents’ Oral Health Knowledge and Attitudes on Oral Health Practices of Children (5-12 Years) in a Rural School in KwaZulu Natal, South Africa: A Cross Sectional Survey

Prenisha Neupaul, Ozayr Mahomed

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: knowledge; attitude; practice; children’s oral health practice; school going children; rural area

Online: 21 May 2020 (04:01:10 CEST)

Show abstract| Download PDF| Share

Introduction: Oral health knowledge, beliefs and attitudes of parents have a direct influence on their child’s oral health maintenance, dietary habits and encourage healthy behaviors. The aim of this study was to assess the knowledge, and attitude of parents with regards to the oral health practice of their children and its associated factors. Methods: A self -administered structured questionnaire was administered to parents or guardians of learners aged 5 to 12 years at a low socio-economic rural primary school in the uGu district, KwaZulu Natal, South Africa to collect demographic variables, knowledge of dental health, use of toothbrush, use of toothpaste, dietary practices and dental visits; practice with respect to dental care and attitudes towards oral health. The questionnaire was translated from English to IsiZulu was used. Bivariate and multivariate logistic regressions were used to assess the association between parent’s sociodemographic factors with their oral health knowledge and practice scores. Results: One hundred and forty four completed survey instruments were received with an eighty percent (118) representation of mothers. Ninety five percent of the parents (136) had a positive attitude towards oral health with 86% (124) of the children brushed their tongue and 89% (128) of the children brushed their teeth happily. The mean knowledge score was 70% with a median of 72%. Children were significantly more likely to brush their tongues (AOR: 3.20 95% CI: 1.06-9.66) and were more likely to be happier when brushing their teeth (AOR: 4.65 95% CI: 1.41-15.38) when the caregivers were their mothers, and when parents had an above average knowledge score (AOR: 1.86 95% CI: 0.72-4.85) and had positive attitudes (AOR: 3.20 95%CI: 0.46-22.00). Conclusion: To reduce the gaps in knowledge, oral health promotion should be integrated into all point of care contact with parents at health facilities, increased community awareness and advertising campaigns as well as a more focused school health oral program that addresses primary prevention, screening and appropriate referrals to health facilities

Preprint ARTICLE | doi:10.20944/preprints202004.0390.v1

O-β-GlcNAcylation, Chloroquine and 2-Hydroxybenzohydrazine May Hamper SARS-CoV-2 entry to Human via Inhibition of ACE2 Phosphorylation at Ser787 but Also Induce Disruption of Virus-ACE2 Binding

Waqar Ahmad, Khadija Shabbiri, Nazarul Islam

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; SARS-CoV; ACE2; spike protein; phosphorylation; O-β-GlcNAcylation; molecular docking; chloroquine; 2-hydroxybenzohydrazine

Online: 22 April 2020 (06:01:00 CEST)

Show abstract| Download PDF| Share

The novel coronavirus COVID- 19 disease is extremely contagious and has been spread worldwide. First COVID-19 case was identified in December, 2019 and within three months, more than one million affected cases and over 65,000 deaths have been reported. SARS-coronavirus 2 (SARS-CoV-2) also known as 2019-nCoV is a causative agent of COVID-19 disease and belongs to the SARS CoV (Severe Acute Respiratory Syndrome corona virus) family. The SARS-CoV-2 enters the human body by binding its viral surface spike protein with the host angiotensin-converting enzyme 2 (ACE2) receptors and cause infection. To prevent the virus entry and its transmission in the human body, we focused on the two domains of ACE2: i) the N-terminal extracellular binding domain (18-740 residues) reported for coronavirus spike interaction, and ii) the C-terminal cytoplasmic region (762-805 residues) to prevent the virus transmission. Therefore, we proposed: i) inhibition of receptor binding domain (RBD) of SARS-CoV-2 and human ACE2 protein may prevent the virus entry to the host and ii) inhibition of phosphorylation at Ser-787 of ACE2 protein may prevent the transmission of the virus in the COVID-19 patients. In the past, the critical role of Ser 787 in human ACE2 protein has been experimentally verified in SARS-CoV transmission, that upon binding to the receptor, SARS- CoV induces CKII- mediated phosphorylation of ACE2 at Ser-787 that in-turn facilitate virus entry to host cells, followed by replication and activation of ACE2, initiates downstream signaling leading to lung fibrosis. Therefore, in this study, we have suggested post-translational modification (PTM) O-β-GlcNAcylation, and two compounds Chloroquine and 2-hydroxybenzohydrazine might share the common pathways to prevent the COVID-19 infection in human. The addition of O-β-GlcNAcylation at same or neighboring Ser/ Thr residues results in phosphorylation inhibition and a change in protein structural and functional confirmations. Thereby, using neural networking methods, we have identified Ser/ Thr residues in ACE2 that are potential sites for phosphorylation and / or O-β-GlcNAcylation. Molecular docking showed that UDP-GlcNAc has more binding affinity with Ser-787 than the phosphoryl group. Moreover, chloroquine and 2-hydroxybenzohydrazine also showed great potential to bind at Ser-787 that may result in inhibition of Ser-787 phosphorylation and downstream signaling. Furthermore, O-β-GlcNAcylation, chloroquine and 2-hydroxybenzohydrazine showed their high affinity at ACE2-SARS-CoV-2receptor binding domain that may prevent the entry of SARS-CoV-2 into human body. In conclusion, inhibition of human ACE2 phosphorylation at Ser-787 and ACE2-SARS-CoV-2 binding domain could be promising targets against SARS-CoV-2 infection.

Preprint ARTICLE | doi:10.20944/preprints202003.0462.v1

The Effect of Parenteral or Oral Iron Supplementation on Fatigue, Sleep, Quality of Life and Restless Legs Syndrome in Iron Deficient Blood Donors: A Secondary Analysis of the Ironwoman Rct

Susanne Macher, Cornelia Herster, Magdalena Holter, Martina Moritz, Eva Maria Matzhold, Tatjana Stojakovic, Thomas R. Pieber, Peter Schlenke, Camilla Drexler, Karin Amrein

Subject: Medicine And Pharmacology, Dietetics And Nutrition Keywords: iron; blood donation; restless legs syndrome; quality of life; sleep; fatigue

Online: 31 March 2020 (22:32:59 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202003.0448.v1

Genomic Characterization of ESBL-producing Escherichia coli Isolates Belonging to a Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 eae-beta1 Occurred in Human Diarrheagenic Isolates, Meat, Poultry and Wildlife

Dafne Díaz-Jiménez, Isidro García-Meniño, Alexandra Herrera, Vanesa García, Ana María López-Beceiro, María Pilar Alonso, Jorge Blanco, Azucena Mora

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; ESBL; hybrid pathotype; ExPEC; EPEC; MDR; ST10; O153; Enterobase

Online: 31 March 2020 (09:51:05 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201910.0012.v1

Analysis and Countermeasures on Paradox of “Poverty Caused by Education” under the Background of Rural Revitalization—Based on the Study of the Poor Population in X County in Southern Shaanxi

Jiaxin Niu, Bin Zhou, Dongpin Hou

Subject: Social Sciences, Education Keywords: rural revitalization; poverty caused by education; economic development

Online: 2 October 2019 (03:37:01 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201906.0186.v1

The Distal and Local Volcanic Ash in the Late Pleistocene Sediments of the Termination I Interval at the Reykjanes Ridge, North Atlantic, Based on Study of the Core AMK-340

Alexander Matul, Irina F. Gablina, Tatyana A. Khusid, Natalya V. Libina, Antonina I. Mikhailova

Subject: Environmental And Earth Sciences, Geophysics And Geology Keywords: tephra in marine sediments; Ash Zone I in North Atlantic; tephrochronology of Termination I

Online: 19 June 2019 (15:38:40 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201903.0077.v1

Effect of Gamma-Ray Irradiation on the Growth of Au Nano-Particles Embedded in the Germano-Silicate Glass Cladding of the Silica Glass Fiber and its Surface Plasmon Resonance Response

Seongmin Ju, Won-Taek Han

Subject: Chemistry And Materials Science, Nanotechnology Keywords: γ-ray irradiation; surface plasmon resonance; fiber sensor; nano-particles; cladding embedded optical fiber

Online: 6 March 2019 (11:37:09 CET)

Show abstract| Download PDF| Share

Preprint CASE REPORT | doi:10.20944/preprints201810.0491.v1

CMV-Specific Immune Reconstitution after Allogenic Stem Cell Transplantation: Central Role of Specific IgG in Immunodefense Against CMV Reactivation as a Leading Parameter during Profound and Long-Lasting Immune Deficiency

Przemyslaw Zdziarski

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: cytomegalovirus (CMV); infection, reactivation, epidemiology, host-virus interaction, CMV-specific IgG; protective IgG level, avidity, adoptive/acquired immune response, hematopoietic stem cell transplantation (HSCT); secondary immunodeficiency, Quantiferon, pentamer, β2-microglobulin/beta 2-Microglobulin

Online: 22 October 2018 (11:53:47 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201810.0428.v1

Is the Response of Tumours Dependent on Dietary Input of Some Amino Acids or on the Ratios Among Essential and Non-Essential Amino Acids? All That Glisters is Not Gold

Francesco Dioguardi, Vincenzo Flati, Giovanni Corsetti, Evasio Pasini, Claudia Romano

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: amino acids, cancer, energy metabolism, autophagy, apoptosis, glutamine, diabetes type 2.

Online: 18 October 2018 (16:45:01 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201809.0289.v1

The Role of Aberrations in the Immune-inflammatory Reflex System (IRS) and the Compensatory Immune-regulatory Reflex System (CIRS) in Different Phenotypes of Schizophrenia: The IRS-CIRS Theory of Schizophrenia

Chutima Roomruangwong, Cristiano Noto, Buranee Kanchanatawan, George Anderson, Marta Kubera, Andre F. Carvalho, Michael Maes

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: schizophrenia, immune system, inflammation, cytokines, immune regulatory, CIRS, psychiatry, immunology, psychosis

Online: 17 September 2018 (08:57:53 CEST)

Show abstract| Download PDF| Share

In this paper we propose a novel theoretical framework, which was previously developed for major depression and bipolar disorder, namely the compensatory immune-regulatory reflex system (CIRS), as applied to the neuro-immune pathophysiology of schizophrenia and its phenotypes, including first episode psychosis (FEP), acute relapses, chronic and treatment resistant schizophrenia (TRS), comorbid depression, and deficit schizophrenia. These schizophrenia phenotypes and manifestations are accompanied by increased production of positive acute phase proteins, including haptoglobin and α2-macroglobulin, complement factors, and macrophagic M1 (IL-1β, IL-6 and TNF-α), T helper (Th)-1 (interferon-γ and IL-2R), Th-2 (IL-4, IL-5), Th-17 (IL-17) and T regulatory (Treg; IL-10 and transforming growth factor (TGF)-β1) cytokines, cytokine-induced activation of the tryptophan catabolite (TRYCAT) pathway as well as chemokines, including CCL-11 (eotaxin), CCL-2, CCL-3 and CXCL-8. While the immune profiles in the different schizophrenia phenotypes indicate activation of the immune-inflammatory response system (IRS), there are simultaneous signs of CIRS activation, including increased levels of the IL-1 receptor antagonist (sIL-1RA), sIL-2R and tumor necrosis factor-a receptors, Th-2 and Treg phenotypes with increased IL-4 and IL-10 production, and increased levels of TRYCATs and haptoglobin, α2-macroglobulin and other acute phase reactants, which have immune-regulatory and anti-inflammatory effects. Signs of activated IRS and CIRS pathways are also detected in TRS, chronic and deficit schizophrenia indicating that these conditions are accompanied by a new homeostatic setpoint between upregulated IRS and CIRS components. In FEP, increased baseline CIRS activity is a protective factor which may predict favorable clinical outcomes. Moreover, impairments in the CIRS are associated with deficit schizophrenia and greater impairments in semantic and episodic memory. It is concluded that CIRS plays a key role in the pathophysiology of schizophrenia by negatively regulating the primary IRS and contributing to recovery from the acute phase of illness. Components of the CIRS may offer promising therapeutic targets for schizophrenia.

Preprint ARTICLE | doi:10.20944/preprints201803.0089.v1

The Relative Influence of Inter-Generational Co-Residence on Healthcare Market and Labor Market Outcomes in Post Affordable-Care-Act USA: Evidence from the 2015–2016 National Health Interview Surveys

Ibrahim Niankara

Subject: Business, Economics And Management, Economics Keywords: Affordable Care Act; access to care; co-residence; labor supply; medical expenditure

Online: 12 March 2018 (08:36:46 CET)

Show abstract| Download PDF| Supplementary Files| Share

This paper investigates the effects of Inter-generational co-residence on health care market, and labor market outcomes, to see whether or not living under the same roof with at least one parent matters in health care market, and labor market behaviors in post-Affordable-Care Act (ACA) USA. The adopted analytical strategy involves not only looking at the gender differences in co-residence, and its effects on outcomes in the two markets, but also accounting for co-residence endogeneity following the recent literature. Unlike the recent literature that relies on instrumental variables methods, this study adopts a switching regression approach, defining inter-generational co-residence as an endogenous selection process using a binary probit equation, and modeled jointly with the extensive margins and intensive margins in the two markets. This novel approach results in a recursive trivariate probit model for each market, and estimated using penalized maximum likelihood methods. The results suggest that ACA by reorganizing the US health care market, seems to have reduced significantly disparities in health care access among males and females based on race, region of residence, place of birth, and citizenship. However not only do we observe significant differences in inter-generational co-residence status between males and females, we also find significant inequalities in the effects of co-residence on health care market, and labor market outcomes. In fact, co-residence is found to increase health care expenditure by 56.7% among females, while this figure increases to 74.2% among males. In addition co-residing individuals, while spending 69.7% more on health care annually are 1.22 times more likely to access health care, but 31% less likely to use health care intensively during the year. In the labor market, co-residence is found to reduce significantly hours of weekly labor supplied by 41% for females, and 55.6% for males. Furthermore co-residing individuals, while not significantly different in their likelihood of labor force participation, are 1.52 times less likely to work full time once they decide to participate, and also spend about 55.4% less time working in the labor market in post-ACA USA.

Preprint ARTICLE | doi:10.20944/preprints201710.0141.v1

A Case Study on Large Deformation Failure Mechanism of Coal given Chamber and Invention of a New Wall-Mounted Coal Bunker in Xiashijie Coal Mine with Soft, Swelling Floor Rock

Xingkai Wang, Wenbing Xie, Shengguo Jing, Jianbiao Bai, Zhili Su

Subject: Engineering, Control And Systems Engineering Keywords: Vertical coal bunker; Coal given chamber; Floor heave; Wall-mounted coal bunker; Reinforcement; Self-bearing system

Online: 20 October 2017 (15:31:57 CEST)

Show abstract| Download PDF| Share

Serious damage caused by floor heave in the coal given chamber of a vertical coal bunker is one of the challenges faced in underground coal mines. Engineering practice shows that it is more difficult to maintain the coal given chamber (CGC) than a roadway. More importantly, repairing the CGC during mining practice will pose major safety risks and reduce production. Based on the case of the serious collapse that occurred in the bearing structure of the CGC at the lower part of the 214# coal bunker in Xiashijie mine, China, this work analysed (i) the main factors influencing floor heave and (ii) the failure mechanism of the load-bearing structure in the CGC using FLAC^2D numerical models and expansion experiment. The analysis results indicate that: the floor heave, caused mainly by mine water, is the basic reason leading to the instability and repeated failure of the CGC in the 214# coal bunker. Then a new coal bunker, without building the CGC, is proposed and put into practice to replace the 214# coal bunker. The FLAC^3D software program is adopted to establish the numerical model of the wall-mounted coal bunker (WMCB), and the stability of the rock surrounding the WMCB is simulated and analysed. The results show that: (1) the rock surrounding the sandstone segment is basically stable. (2) The surrounding rock in the coal seam segment, which moves into the inside of the bunker, is the main zone of deformation for the entire rock mass surrounding the bunker. Then the surrounding rock is controlled effectively by means of high-strength bolt–cable combined supporting technology. According to the geological conditions of the WMCB, the self-bearing system, which includes (i) H-steel beams, (ii) H-steel brackets, and (iii) self-locking anchor cables, is established and serves as a substitute for the CGC to transfer the whole weight of the bunker to stable surrounding rock. The stability of the new coal bunker has been verified by field testing, and the coal mine has gained economic benefit to a value of 158.026174 million RMB over three years. The new WMCB thus made production more effective and can provide helpful references for construction of vertical bunkers under similar geological conditions.

Preprint ARTICLE | doi:10.20944/preprints201705.0156.v1

The Normal Human Endometrium but not Myometrium Presents Menstrual Cycle-Dependent Fluctuations in the Immunoexpression of DNA Fragmentation Factor 40, DNA Fragmentation Factor 45, and B-cell Lymphoma 2 Protein.

Tomasz Banas, Kazimierz Pitynski, Krzysztof Okon, Marcin Mikos, Joanna Bonior, Artur Ludwin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: DNA fragmentation factors 40, DFF40, DNA fragmentation factors 45, DFF45, b-cell lymphoma 2, bcl-2, endometrium, menstrual cycle, uterine myometrium.

Online: 22 May 2017 (05:44:31 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202410.1324.v1

The Impact of the SMOTE Method on Machine Learning and Ensemble Learning Performance Results in Addressing Class Imbalance in Data Used for Predicting Total Testosterone Deficiency in Type 2 Diabetes Patients

Mehmet Kıvrak, Ugur Avci, Hakkı Uzun, Cüneyt Ardıç

Subject: Artificial Intelligence And Machine Learning, Computer Science And Mathematics Keywords: SMOTE; inbalance problem; total testosterone; machine learning; ensemble learning

Online: 16 October 2024 (19:50:49 CEST)

Show abstract| Download PDF| Share

Background and Objective: Diabetes Mellitus is a long-term, multifaceted metabolic condition that necessitates ongoing medical management. Hypogonadism is a syndrome that is a clinical and/or biochemical indicator of testosterone deficiency. Cross-sectional studies have reported that 20-80.4 % of all men with Type 2 diabetes have hypogonadism, and Type 2 diabetes is related with low testosterone. This study presents an analysis of the use of ML and EL classifiers in predicting testosterone deficiency. In our study, we compared optimized traditional ML classifiers and three EL classifiers using grid search and stratified k-fold cross-validation. We used the SMOTE method for the class imbalance problem. Methods: This database contains 3397 patients for the assessment of testosterone deficiency. Among these patients, 1886 patients with type 2 diabetes were included in the study. In the data pre-processing stage, firstly outlier/excessive observation analyses were performed LOF and missing value analyses were performed with random forest. The SMOTE is a method for generating synthetic samples of the minority class. Four basic classifiers, namely MLP, RF, ELM and LR, were used as first level classifiers. Tree ensemble classifiers, namely ADA, AGBoost, and SGB, were used as second level classifiers. Results: After SMOTE, while the diagnostic accuracy decreased in all base classifiers except ELM, sensitivity values increased in all classifiers. Similarly, while the specificity values decreased in all classifiers, F1 score increased. The RF classifier gave more successful results on the base-training dataset. The most successful ensemble classifier in the training dataset was the ADA classifier in the original data and in the after SMOTE data. The testing data, XGBoost is the most suitable model for your intended use in evaluating model performance. XGBoost, which exhibits a balanced performance especially when SMOTE is used, can be preferred to correct class imbalance. Conclusions: SMOTE is used to correct the class imbalance in the original data. However, as seen in this study, when SMOTE was applied, the diagnostic accuracy decreased in some models, but the sensitivity increased significantly. This shows the positive effects of SMOTE to better predict the minority class.

Preprint ARTICLE | doi:10.20944/preprints202410.1118.v1

Introducing the Second-Order Features Adjoint Sensitivity Analysis Methodology for Neural Ordinary Differential Equations. II: Illustrative Application to Heat and Energy Transfer in the Nordheim-Fuchs Phenomenological Model for Reactor Safety

Dan Gabriel Cacuci

Subject: Engineering, Safety, Risk, Reliability And Quality Keywords: neural ordinary differential equations; second-order adjoint sensitivity analysis; first-order and second-order sensitivities of decoder responses with respect to features of model parameters; Nordheim-Fuchs phenomenological model for reactor safety; energy release decoder response; heat transfer decoder response

Online: 15 October 2024 (12:34:43 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202410.0382.v1

Healthcare Settings and Infections Prevention: Today’s Proce-dures in Light of the “Instructions for Disinfection” Issued During the 1817 Typhus Epidemic in the Grand Duchy of Tus-cany (Pre-Unification Italy)

Davide Orsini, Maria Luisa Cristina, Anna Maria Spagnolo, Carola Minet, Marina Sartini, Roberto Parrella, Nicola Luigi Bragazzi, Mariano Martini

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: infections; prevention; typhus; epidemics; XIX century; Grand Duchy of Tuscany; history of medicine

Online: 7 October 2024 (11:17:56 CEST)

Show abstract| Download PDF| Share

Search Results

39546 articles found