Smart farm is a technology that greatly helps improve the productivity and quality of crops and is being actively introduced into indoor environments such as green houses. However, it has not yet been distributed to field-grown crops. The main reason is that, unlike indoor environments, it is very difficult to collect sensor data for monitoring the growth environment of crops in open fields where weather conditions change significantly and power supply is difficult. Additionally, because various sensors are used, the data formats of the devices are different, making it difficult to process. This paper presents a field crop growth environment monitoring and big data analysis system. The proposed system first solves power supply and data communication problems using solar power generation and LPWA technology. Additionally, based on the oneM2M architecture, data from various sensors is transmitted to the server using standardized technology. The transmitted data is stored and managed on the server as big data, and can be used to predict the production and quality of field-grown crops and take appropriate measures. The proposed system is expected to create an environment optimized for the growth of field crops and help prevent and manage diseases.

Natural vegetation restoration can take decades to achieve and requires information on species composition and trend to inform management strategies. Here we demonstrate the potential and portability of drone, dove and Sentinel-2 imagery for species level classification and trajectory monitoring using three study sites with different revegetation histories. Drone imagery (4 cm) was classified using random forests. Dove (3 m) and Sentinel-2 (10 m) images were acquired close to the anniversary of the drone flights and converted into fractional cover maps showing the proportion of exotics, natives and other land covers using ordinary least squares (OLS) and geographically weighted regression (GWR). The time series of fractional cover maps were converted into positive and negative slope and second-order curvature and summarised as one RGB composite to illustrate trend and observe exotic expansion. Drone classification accuracies ranged from 73%-76% when applied to the full plant species list and between 93% and 97% when grouped into natives, exotics, and other land covers. GWR outperformed OLS for all fractional cover mapping and GWR with dove imagery was more accurate than with Sentinel imagery. The combination of very high-resolution drone acquired imagery with the versatility of dove acquired imagery arms land managers with highly accurate species composition and their spatio-temporal dynamics to promote timely intervention.

This study was to explore the effects of glabridin and gymnemic acid on the structural changes of the choroidal layer as well as the expression of VEGF and CD31 in diabetic rats. The animals were separated into five groups: the control group (C), the diabetic group (DM), the diabetic rats treated with glabridin 40 mg/kg body weight (DM+GB), the diabetic rats treated with gymnemic acid 400 mg/kg body weight (DM+GM), and the diabetic rats treated with glyburide 4 mg/kg body weight (DM+GR). There has been an increase in the thickness of both the choroid layer and the wall of the arteries in the diabetic group. A decrease in vascularity and choroidal neovascularization can be found in DM rats. After eight weeks of experimentation, the choroidal thickness and wall of choroid arteries, as well as the diameter of the lumen of choroid arteries in the DM+GB, DM+GM, and DM+GR groups, had improved. The expression of VEGF and CD31 was lower compared to the DM group. According to the findings of the current research, glabridin and gymnemic acid can improve and reduce the damage to the choroid, which is a factor that can sometimes result in vision loss.

Dimers and oligomers of alkenes represent a category of compounds that are in great demand for diverse industrial sectors. Among the developing synthetic methods, the catalysis of alkene dimerization and oligomerization using transition metal salts and complexes is of undoubted interest for practical application. This approach demonstrates substantial potential, offering not only elevated reaction rates but also precise control over the chemo-, regio-, and stereoselectivity of the reactions. In this review, we discuss the data on catalytic systems for alkene dimerization and oligomerization. Our focus lies in the analysis of how the activity and chemoselectivity of these catalytic systems are influenced by various factors, such as the nature of the transition metal, the ligand environment, the activator, and substrate structure. Notably, the review particularly discusses the reaction mechanisms, encompassing metal complex activation, structural and dynamic features, and the reactivity of hydride intermediates, which serve as potential catalytically active centers in alkene dimerization and oligomerization.

Long COVID, also known as post-acute sequelae of SARS-CoV-2 infection (PASC), has emerged as a significant health concern following the COVID-19 pandemic. Molecular mechanisms underlying the occurrence and progression of long COVID include viral persistence, immune dysregulation, endothelial dysfunction, and neurological involvement, and highlight the need for further research to develop targeted therapies for this condition. While a clearer picture of the clinical symptomatology is shaping, many molecular mechanisms are yet to be unraveled, given their complexity and high level of interaction with other metabolic pathways. This review summarizes some of the most important symptoms and associated molecular mechanisms that occur in long COVID, as well as the most relevant molecular techniques that can be used in understanding the viral pathogen, its affinity towards the host and the possible outcomes of host-pathogen interaction.

The decade of research concentrating on the area of Easter Slavonia revealed abundance of large on complex late Neolithic sites. It changed profoundly how we perceive late Neolithic settlement and space size and organization. As s first step in particular site detection we mostly conducted aerial reconnaissance and satellite image analysis but also data from the field such as excavation. On detected potential sites we conducted intensive field surveys and confirmed the attribution to the late Neolithic by surface finds. On those confirmed sites in the vicinity of Đakovo, Croatia, magnetic survey was conducted on 5 sites so far and the results confirmed presence of large scale late Neolithic settlements with complex spatial organization and enclosure(s). Most complex remains so far is site Gorjani Kremenjača and Topole which we present in this paper where a remains of one or two settlements covers the area of 70 hectares and is currently in the process of formal protection as a cultural landscape by the Ministry of Culture and Media of the Republic of Croatia. Special focus of the paper is the application of remote sensing in detection, archaeological confirmation and protection of the position Gorjani Topole.

This study focusses on the geological formations of the Alfallé massif located approximately 75km east of the town of N'Goura, in the Hadjer-Lamis Province of Chad (13°N, 17°E), and their ability to provide good quality aggregates in an area with poor hard rock ressources. We investigated the petrographic and mechanical characteristics of these formations in a resource perspective. The massif is made up of three lithologies: alkali feldspar megacrystalline granite, biotite-rich fine grained quartz monzonite and biotite-rich fine grained gneiss. Alkali feldspar megacrystalline granite is composed of quartz (30%), orthoclase (30%), plagioclase (20%), biotite (15%) and opaque minerals (≈5%). Micro-monzonite is composed of quartz (15-20%), orthoclase (20-25%), plagioclase (18-25%), biotite (20-25%) and opaque minerals (≈4%). The biotite gneiss is composed of quartz (25-30%), alkali feldspars (25-27%), plagioclase (17%), biotite (25-27%) and opaque minerals (≈5%). The mechanical characteristics of the aggregates show that the aggregates studied have specific density values of between 2.58 and 2.75g/cm3 with an average value of 2.66g/cm3, a Los-Angeles coefficient of 11.2% to 28.9% with an average value of 20.1% and a wet Micro-Deval coefficient of 5.2% to 10.8% with an average value of 7.6%. The values obtained indicate that the materials studied have excellent to satisfactory properties, and that Alfallé massif provide the best characteristics among six different quarrying area in Central Chad. We propose that such rare residual reliefs within the mega-Chad paleolake area reach optimal mechanical properties through a natural selection process, specific of the paleolake erosional conditions.

The present paper presents findings from the follow-up of 185 of these nurses who had been involved in the front-line care of COVID-19 patients in urban centres in Northern England, during and after the “second wave” of COVID patients in November, 2020. Measures of adjustment, stress (including PTSD), and Maslowian self-actualization were added to earlier measures of personality, adjustment, work-life stress, and career intention. Cluster analysis identified three types in the 2020-2023 cohort: I ‘Actualizing Professionals’; (N=62); II ‘Strong Professionals’ (N=56); III ‘Highly Stressed Nurses’ (N=45). These latter very stressed nurses included all nurses (N=16) identified as having PTSD symptoms.

In this work, backscattering signatures of snow-covered lake ice and sea ice from X- and Ku-band synthetic aperture radar (SAR) data are investigated. The SAR data were acquired with the ESA airborne SnowSAR sensor in winter 2012 over Lake Orajärvi in northern Finland and over landfast ice in the Bay of Bothnia of the Baltic Sea. Co-incident with the SnowSAR acquisitions in-situ snow and ice data were measured. In addition, time series of TerraSAR-X images and ice mass balance buoy data were acquired for the Lake Orajärvi in 2011-12. The main objective of our study was to investigate relationships between SAR backscattering signatures and snow depth over lake and sea ice, with the ultimate objective of assessing the feasibility of retrieval of snow characteristics using X- and Ku-band dual-polarisation (VV and VH) SAR over freshwater or sea ice. This study constitutes the first comprehensive survey of snow backscattering signatures at these two combined frequencies over both lake and sea ice. For lake ice, we show that X-band VH-polarized backscattering coefficient (σo) and the Ku-band VV/VH-ratio exhibited the highest sensitivity to the snow depth. For sea ice, the highest sensitivity to the snow depth was found from the Ku-band VV-polarised σo and the Ku-band VV/VH-ratio. However, the observed relations were relatively weak, indicating that at least for the prevailing snow conditions, obtaining reliable estimates of snow depth over lake and sea ice would be challenging using only X- and Ku-band backscattering information.

The study aimed to isolate and characterize bacteriocin-producing Enterococcus species from Ethiopian traditional fermented foods and beverages. The bacteriocin bioassay was tested against food-borne pathogens at various temperatures, pH, solvents, additives, and enzymes. A solvent extraction method was established to concentrate bacteriocins from Enterococcus species. The study found that crude extracts with proteinase K, pepsin, and trypsin deactivated antimicrobial activity, confirming their proteinaceous nature. Organic solvents were more effective at extracting bacteriocins from CFS, with chloroform alone and in combination providing rapid and efficient recovery. The optimal bacteriocin production and strong inhibition activity were achieved at 37°C with a pH range of 5.5 to 6.5 incubated for 16-18 hours. The six Enterococcus faecium species were the best bacteriocin-producing isolates. Optimizing bacteriocin production and changing environmental growing circumstances can enhance the application of bacteriocins as food preservation agents. Traditional fermented products are potential sources of bacteriocin-producing LAB.

Taking Korla fragrant pear as the research object, single factor testing and water and fertilizer coupling testing were conducted. Under the premise of the drip irrigation mode, the effects of water, nitrogen, and phosphorus on the growth, fruit quality, and yield of Korla Fragrant Pear were studied to provide a theoretical basis for efficient water and fertilizer management. Among them, the single factor test set water, nitrogen and phosphorus as the three factors, and each factor was set at five levels, namely, water (W1, 5460 m3·hm−2;W2, 5880 m3·hm−2;W3,6300 m3·hm−2; W4, 6720 m3·hm−2; W5, 7140 m3·hm−2), nitrogen (N1, 150 kg N·hm−2;N2, 225 kg N·hm−2; N3, 300 kg N·hm−2; N4, 375 kg N·hm−2; N5, 450 kg N·hm−2), phosphorus (P1,75 kg P2O5·hm−2; P2, 150 kg P2O5·hm−2; P3, 225 kg P2O5·hm−2; P4, 300 kg P2O5·hm−2; P5, 375 kg P2O5·hm−2). The results showed that the total nitrogen and total phosphorus contents in leaves under the W3, W4, N3, N4, P3, and P4 treatments were significantly higher than those under other treatments, and the fruit quality was better. Therefore, the W3, W4, N3, N4, P3, and P4 treatments were selected as the water and fertilizer inputs for the water–fertilizer coupling test for Korla fragrant pear. Eight treatments were set up in the water and fertilizer coupling test: Treatment 1 (T1, W3N3P3), Treatment 2 (T2, W3N3P4), Treatment 3 (T3, W3N4P3), Treatment 4 (T4,W3N4P4), Treatment 5 (T5, W4N3P3), Treatment 6 (T6, W4N3P4), Treatment 7 (T7, W4N4) P3), and Treatment 8 (T8, W4N4P4). The results showed that the leaf area index of the T1, T2, T3, and T4 treatments was significantly higher than that of the other treatments at maturity. The T3 treatment resulted in the highest fruit yield, single fruit weight, and primary fruit rate. The results of the grey correlation degree analysis of fruit quality showed that T3 treatment had the highest degree of correlation and ranking of each fruit quality index, indicating that T3 treatment had the highest fruit quality. Detailed investigation of pear tree growth and fruit quality showed that the T3 treatment (375 kg·hm−2 nitrogen application, 225 kg·hm−2 phosphorus application and 6300 m3·hm−2 irrigation) had the best fruit growth and development performance, and the sweet pear fruit quality was the best.

Food allergies are a growing public health concern worldwide, especially in children and young adults. Allergen-specific IgE plays a central role in the pathogenesis of food allergies, but their titers poorly correlate with allergy development. Host immune systems yield allergen-specific immunoglobulin (Ig)A, IgE and IgG subclasses with low or high affinities and differential Fc N-glycosylation patterns that can affect the allergic reaction to food in multiple ways. High affinity IgE is required to induce strong mast cell activation eventually leading to allergic anaphylaxis while low affinity IgE can even inhibit the development of clinically relevant allergic symptoms. IgA and IgG antibodies can inhibit IgE-mediated mast cell activation through various mechanisms, thereby protecting IgE-positive individuals from allergy development. The production of IgE and IgG with differential allergenic potential seems to be affected by the signaling strength of individual B cell receptors, and by cytokines from T cells. This review provides an overview of the diversity of the B cell response and the diverse roles of antibodies in food allergy.

The roots of African grapes (Lannea microcarpa) have long been utilised in traditional medicine for their perceived therapeutic properties. However, their comprehensive proximate and mineral analysis remains largely unexplored. Proximate composition and mineral content of the roots were conducted. The sample was obtained from Katsayal, Katsina State, and analysed using standard procedures. The macro and micro metals were determined using Flame Photometer and Atomic Absorption Spectroscopy, respectively. The results revealed a moisture content of 28.0 ±2.0%, an ash content of 21.3±1.2%, and significant levels of crude fibre (29.0±0.50%), crude lipid (9.60±0.20%), crude protein (6.91±0.01%), and available carbohydrates (33.3±0.10%). The calorific value of the root was determined to be 245 ± 0.01 kcal/g. The micro-mineral analysis revealed the detection of copper (8.60 ± 0.002 mg/kg), magnesium (9.24 ± 0.004 mg/kg), manganese (41.9 ± 0.001 mg/kg), iron (405 ± 0.042 mg/kg), and zinc (9.46 ± 0.001 mg/kg). The macro-minerals, namely calcium (28400 mg/kg), potassium (1530 mg/kg), and sodium (45030 mg/kg), were found to be present in the sample. It is advisable to conduct additional research to examine the identified minerals' bioavailability, clarify the phytochemical profile, and investigate potential bioactive compounds.

Neutrophils are the principal trouper of innate immune system. Activated neutrophils undergo a noble cell death termed NETosis and release a mesh-like structure called neutrophil extracellular traps (NETs) as a part of their defensive strategy against microbial pathogen attack. This web-like architecture includes a DNA backbone embedded with antimicrobial proteins like myeloperoxidase (MPO), neutrophil elastase (NE), histones etc. and deploys in the entrapment and clearance of encountered pathogens. Thus NETs play an inevitable beneficial role in the host's protection. However, recent accumulated evidence shows that dysregulated and enhanced NET formation has various pathological aspects including promotion of sepsis, pulmonary, cardiovascular, hepatic, nephrological, thrombotic, autoimmune, pregnancy, cancer diseases etc. and the list is increasing gradually. In this review, we summarize NETs mediated pathophysiology of different diseases, focus on some updated potential therapeutic approaches against NETs and share our future perspectives.

The macro-litter (plastic) sea bottom pollution of 14 city harbors and marinas in North Africa and in the western Baltic Sea was investigated using a new simple mobile underwater camera system. The study was complemented by a harbor manager survey and 3D-hydrodynamic transport simulations. The average pollution in German marinas was 0.1 particles/m² sea floor (0.04–1.75). The pollution in North African marinas on average was 7 times higher (0.7 particles/m²) and exceeded 3 particles/m² in city center harbors. The resulting >100,000 litter particles per harbor indicate the existence of a problem. With 73%-74%, plastic particles are dominating. Existing legal and management frameworks explain the lack of plastic bottles and bags on sea floors in Germany and are one reason for the lower pollution levels. Items that indicate the role of untreated sewage water were not found. Harbor festivals seem not to be quantitatively relevant for open sea bottom pollution. Our method tends to underestimate the pollution level. Model simulations indicate that storms can cause litter reallocations and sediment cleanings. However, marina sea floor monitoring is recommendable, because it addresses pollution hot-spots, is cost-effective and takes place close to emission sources. Further, the effectiveness of land-based pollution reduction measures can easily be assessed.

Listerias of the phylogenetic lineage II (PLII) are common in the European environment, and are hypovirulent. Despite this, they caused more than a third of sporadic cases of listeriosis, and multi-country foodborne outbreaks. L. monocytogenes ST37 is one of them. During the COVID-19 pandemic ST37 appeared in the clinical cases and ranked second in occurrence among food isolates in the Moscow region. The aim of this study was to describe the genomic features of ST37 isolates from different sources. All clinical cases of ST37 were in the cohort of male patients (age, 48-81 years) with meningitis-septicemia manifestation, and COVID-19 or Influenza in the anamnesis. The core genomes of the fish isolates were closely related. The clinical and meat isolates revealed a large diversity. Prophages (2-4/genome) were the source of the unique genes. Two clinical isolates displayed the pseudolysogeny, and excided prophages were A006-like. Absence of the plasmids, the assortment of virulence factors and resistance determinants in chromosome corresponded to the hypovirulent characteristics. However, all clinical isolates caused severe disease with deaths in four cases. Thus, these studies allow us to speculate that a previous viral infection increases a human susceptibility to listeriosis.

The growing population with changing consumption patterns in developing countries is causing significant challenges with regards to solid waste management. Plastic wastes are particularly problematic, with single-use plastics leaking into the environment, including the marine environment, at an unprecedented rate. Approximately 80 percent of ocean plastics come from land-based sources or about 8 million tons are dumped into the ocean every year. Thailand was identified as one of the top 10 countries ranked by mass of mismanaged plastic wastes, with more than 60,000 tons per year entering the ocean through multiple outlets, including rivers. In the environment, plastic wastes can be degraded into small sizes (less than 5 mm), called microplastics (MPs), which could contaminate the ecosystems and the food chain, including foodstuffs and water supply. Tap water samples collected from a water supply treatment plant and Academic Institutions 1 and 2 in central Thailand were found to contain MPs about 304 ±90, 270 ±109 and 386 ±102 particles/L, respectively. In addition, MPs concentrations of 211 ±70 and 122 ±60 particles/L were also found in drinking water samples collected from commercial bottled water and membrane filtration water, respectively. The MPs sizes of 0-50 µm were most abundant in both the tap and drinking water samples whose shapes were mainly fragments and fiber. These data indicated potential health risks to the people who consume these water sources, and recommendations for health impacts minimization were proposed.

In contrast to some low molar-mass per- and polyfluoroalkyl substances (PFASs), well-established to be toxic, persistent, bioaccumulative and mobile, fluoropolymers (FPs) are water insoluble, safe, bio-inert, durable niche high performance polymers which fulfil the 13 polymer of low concern (PLC) criteria in their recommended conditions of use. In addition, more recent innovations (e.g., the use of non-fluorinated surfactants in aqueous radical (co)polymerization of fluoroalkenes) from industrial manufacturers of FPs are highlighted. This review also aims at showing how these specialty polymers endowed with outstanding properties are essential (even irreplaceable since hydrocarbon polymer alternatives used in similar conditions fail) for our daily life (electronics, Energy, optics, internet of things, transportation, etc) and constitute a special family a part from other “conventional” C1-C10 PFASs found everywhere on the Earth and Oceans. Furthermore, some information reports the recycling (e.g. the unzipping depolymerization of polytetrafluoroethylene, PTFE, into TFE), end of life of FPs, their risk assessment, circular economy and regulations. Various researches are devoted to Environment involving FPs, though they represent a niche volume (with a yearly production of 330,300 tonnes) compared to all plastics (with 460 million tonnes). Complementary to other reviews on PFASs, which lack of such above data, this review presents both fundamental and applied strategies as evidenced by major FP producers.

Soil moisture plays a crucial role in various hydrological processes and energy partitioning of the global surface. The Soil Moisture Active Passive-Sentinel (SMAP-Sentinel) remote sensing technology has demonstrated a great potential in monitoring soil moisture at a scale greater than 1 km. This capability can be applied to improve weather forecast accuracy, enhance water management for agriculture, and climate-related disasters. Despite the techniques increasing used worldwide, its accuracy still requires field validation in specific regions like Thailand. In this paper, we report on extensive in-situ monitoring of soil moisture (from surface up to 1 m depth) at 10 stations across Thailand spanning the years 2021 to 2023. The aim was to validate SMAP surface soil moisture (SSM) Level 2 product over a period of two years. Using one month averaging approach, the study revealed linear relationships between the two measurement types, with the coefficient of determination (R-squared) varying from 0.13 to 0.58. Notably, areas with more uniform land use and topography such as croplands tended to have a better coefficient of determination. We also conducted detailed soil core characterization, including soil-water retention curves, permeability, porosity, and other physics properties. These soil properties were then used for estimating the correlation constants between SMAP and in-situ soil moistures using multiple linear regression. The results demonstrated R-squared values between 0.933 and 0.847. An upscaling approach of SMAP was proposed which showed a promising results when using 3-month average of all measurements in cropland together. The finding also suggest that the SMAP-Sentinel remote sensing technology exhibits significant potential for accurate soil moisture monitoring in diverse applications. Further validation efforts and research, particularly in terms of root zone depths and area-based assessments, especially in the agricultural sector, can greatly improve the technology’s effectiveness and usefulness in the region.

Although the frequency of myocarditis in the general population is very difficult to accurately determine due to the large number of asymptomatic cases, the incidence of this disease is increasing significantly due to better defined criteria for diagnosis and the development of modern diagnostic method. The multitude of different etiological factors, the diversity of the clinical picture and the variability of the diagnostic findings make this disease often demanding both for the selection of the diagnostic modality and for the proper therapeutic approach. The previously known most common viral etiology of this disease is today overshadowed by new findings based on immune-mediated processes, associated diseases that in their natural course can lead to myocardial involvement, but also the iatrogenic cause of myocarditis due to use of immune checkpoint inhibitors in the treatment of cancer patients. Suspecting that a patient with polymorphic and non-specific clinical signs and symptoms, changes in ECG and echocardiography has myocarditis is the starting point in the diagnostic algorithm. Cardio magnetic resonance imaging is non-invasive diagnostic gold standard for diagnosis and clinical follow-up of thses patients. Endomyocardial biopsy as an invasive method is the diagnostic choice in life-threatening patients with suspicion of fulminant myocarditis where the diagnosis has not yet established or there is no adequate response to the applied therapeutic regimen. The treatment of myocarditis is increasingly demanding and includes conservative methods of treating heart failure, immunomodulatory and immunospressive therapy, methods of mechanical circulatory support and heart transplantation. The goal of developing new diagnostic and therapeutic methods is to reduce mortality from this complex disease, which is still high.

Background: The biofilm-forming bacteria Porphyromonas gingivalis (P. gingivalis) is primarily responsible for periodontal disorders, which continue to be a global health issue. Despite the widespread use of antibiotics like metronidazole, other potent therapeutic a crucial quorum sensing molecule implicated in the formation of P. gingivalis biofilm. Approaches are now being explored due to the growing challenge of antibiotic resistance. Objective: In particular, chemicals from Symphytum Officinale (S) and Panax Ginseng (G) plants will be examined in this study to assess if they have the ability to inhibit biofilm and to prevent the development of acylated homoserine lactones (AHLs), a quorum sensing molecule. Materials and Methods: With Metronidazole serving as a control, the antibacterial effects of these substances (Symphytum Officinale and Panax Ginseng) were examined against a standard strain and a clinical isolate of P. gingivalis. Dilutions of these plant extracts were used either alone (G+S) or in conjunction with metronidazole (G+S+F), to assess their antibacterial activity using antibacterial susceptibility test (inhibition zone), biofilm inhibition and disruption assay (optical density) and quorum sensing inhibition assay (AHL). Results: The combinations of Symphytum officinale, Panax Ginseng and metronidazole (S+G+F) showed the maximum effectiveness with highest zone of inhibition (25.000±0.001 mm) and biofilm inhibition (98.46%), and were comparable to G+S (inhibition zone of 24.341±0.593 mm and biofilm inhibition of 97.76%), and significantly different in the degree of biofilm inhibition with different treatment scenarios. Additionally, the plant's extracts and combinations are specific concentrations had a significant effect on the suppression of the generation of AHL (p < 0.05). Conclusion: According to preliminary research, these plant-derived substances, especially when paired with metronidazole, significantly inhibited the growth of P. gingivalis biofilm providing valuable information for the development of innovative therapeutic approaches for periodontitis and other biofilm-associated illnesses.

: There remains no acquiescence that confirms the extent to which unemployment and entrepreneurship influence each other in developing countries. Thus, an incessant invite for further debate attracted the need to assess the relationship between unemployment and entrepreneurship in terms of business formation in Namibia Given that, this study examines the relationship between unemployment and entrepreneurial activities in Namibia. Time series data were collected from the World Bank database to investigate the relationship between unemployment and entrepreneurship in Namibia. Unit root test was conducted using both the Augmented Dickey-Fuller test and the Phillips-Perron test to measure stationarity of the data and found all the variables to have no unit root after the first difference. Thus, we employed the Johansen-Juselius test of cointegration, and the results revealed the absence of a long-run relationship between the variables. Hence, we performed a vector autoregressive (VAR) model to estimate the short-run relationships and found that Namibia exhibit both the refugee and Schumpeter effects. Finally, the direction of causality between the variables was measured using the Pairwise Granger causality test and the results revealed that none of the variables was proven to granger cause the other, concluding that they are all independent of each other. Therefore, in pursuit of unemployment mitigation, we recommend policymakers to focus on other aspects beyond entrepreneurship when devising policies without jeopardizing the ease of business start-up procedures. This will incentivize entrepreneurial activities, which can eventually address other macroeconomic issues beyond unemployment.

Digital music is one of the most important things in market due to the music royalties’ distribution in Korea. As the music market is transformed to the digital music market such as download the music and streaming, the distribution of music royalties starting from online service provider (OSP) has come to be a highly important part for music rights holders. One of the most important issues in current music royalties’ distribution in Korea is the unfair way of music royalties due to indiscriminative repeat streaming of digital music. To prevent this, music log usage data from several OSPs was collected in day-based system, however, there was a limit to identify detailed information on the use of music in current state. This paper analyzed the structural problems and limitations of settlement of music royalties, and provides a structure in which there can be transparent settlement and distribution between users and rights holders as one of the institutional measures. We’ll also propose various AI-based applications using music log usage data. The proposed system will hopefully be used for public purposes.

The chemical composition of stingless bee honey and propolis depends on the plant sources they are derived from, and thus reflects the flora available in the vicinity of the hives, the preference of the bee species, and the climate (altitude and temperature). To understand the relative influence of these factors, we studied the composition of honey and propolis. Samples from 24 colonies were analyzed: 12 from each of two Scaptotrigona mexicana meliponiaries located in the state of Chiapas in southern Mexico approximately 8.5 km apart. The chemical composition of honey and propolis was studied using NMR, and GC-MS, respectively. Antioxidant activity of propolis was also studied. Chemometric analyses were applied. The Tuxtla Chico honey samples contained higher concentrations of glucose and fructose, while the Cacahoatán samples displayed a rich composition of di- and trisaccharides. These differences can be attributed to the distinct nectar sources utilized by the bees in each location. Propolis composition in the two locations also demonstrated qualitative differences, indicating a specific choice of resins by the bees. The observed substantial variations in the chemical composition of propolis and honey of S. mexicana from two relatively close locations support the assumption that bee species cannot be regarded as the most important factor determining their chemistry.

: Seaweeds are among the most important biomass feedstocks for the production of third generation biofuels. They are also efficient in carbon sequestration during growth, and produce a variety of high value chemicals. Given these characteristics together with the relatively high carbohydrate content, seaweeds have been discussed as an ideal means for CO2 capture and biofuel production. Though third generation biofuels have emerged as some of the best alternatives to fossil fuels, there is currently no large-scale production or mainstream use of such liquid fuels due to the many technical challenges and high production costs. The present study describes the concept of coastal marine biorefineries as the most cost-effective and sustainable approach for biofuel production from sea-weeds as well as atmospheric carbon capture and storage (CCS). The suggested refinery system makes use of marine resources, namely seawater, seaweed, and marine microorganisms. Firstly, extensive screening of the current literature was performed to determine which technologies would enable the emergence of such a novel biorefinery system and its merits over conventional refineries. Secondly, the study investigates various scenarios assessing the potential of seaweeds as a means of carbon sequestration. We demonstrate that the removal of 100 Gigatons of excess CO2 using seaweed farms can be achieved in around 4 months to less than 12 years depending on the area under cultivation and the seaweed species. The total bioethanol that could be generated from the harvested biomass is around 8 trillion litres. In addition, high-value chemicals (HVC) that could potentially be recovered from the process represent a considerable op-portunity with multi-billion-dollar commercial value. Overall, coastal marine biorefineries have strong potential for a sustainable green economy and rep-resent a rapid approach for climate change mitigation.

Four leather substrates from different animals were treated by dispersions containing hydrophilic composite silica-hyperbranched poly(ethylene imine) xerogels. Antimicrobial activity was introduced by incorporating silver nanoparticles and /or benzalkonium chloride. The gel precursor solutions were also infused before gelation to titanium oxide powders typically employed for induction of self-cleaning properties. The dispersions from these biomimetically premade xerogels integrate environmentally friendly materials with short coating times. Scanning electron microscopy (SEM) provided information on the powder distribution onto the leathers. Substrate and coating composition were estimated by infrared spectroscopy (IR) and ener-gy-dispersive X-ray spectroscopy (EDS). Surface hydrophilicity and water permeability were assessed by water-contact angle experiments. The diffusion of the leather's initial components and xerogel additives into the water were measured by Ultraviolet-Visible (UV-Vis) spectroscopy. Protection against GRAM- bacteria was tested for Escherichia coli, Pseudomonas aeruginosa, and Klebsiella Pneumoniae against GRAM+ bacteria for Staphylococcus aureus, and Enterococcus faecalis and against fungi for Candida albicans. Antibiofilm capacity experiments were performed against Staphylococcus aureus, Klebsiella Pneumoniae, Enterococcus faecalis, and Candida albicans. The application of xerogel dispersions proved an adequate and economically feasible alternative to the direct gel formation into the substrate’s pores for the preparation of leathers intended for medical uses

Pharmacogenetics and DNA methylation influence therapeutic outcomes and provide insights into potential therapeutic targets for brain-related disorders. To understand the effect of genetic polymorphisms on drug response and disease risk, we analyzed the relationship between global DNA methylation, drug-metabolizing enzymes, transport genes, and pathogenic gene phenotypes in serum samples from two groups of patients: Group A, which showed increased 5-methylcytosine (5mC) levels during clinical follow-up, and Group B, which exhibited no discernible change in 5mC levels. We identified specific SNPs in several metabolizing genes, including CYP1A2, CYP2C9, CYP4F2, GSTP1, and NAT2 that were associated with differential drug responses. Specific SNPs in CYP had a significant impact on enzyme activity, leading to changes in phenotypic distribution between the two patient groups. Group B, which contained a lower frequency of normal metabolizers and a higher frequency of ultra-rapid metabolizers compared to patients in Group A, did not show an improvement in 5mC levels during follow-up. Furthermore, there were significant differences in phenotype distribution between patient Groups A and B for several SNPs associated with transporter genes (ABCB1, ABCC2, SLC2A9, SLC39A8, and SLCO1B1) and pathogenic genes (APOE, NBEA, and PTGS2). These findings appear to suggest that the interplay between pharmacogenomics and DNA methylation has important implications for improving treatment outcomes in patients with brain-related disorders.

Drilling operation is the major cost factor for the industry. Appropriately designed operations are essential for successful drilling. Optimized drilling operations also allow enhanced drilling performance and reduce the drilling cost. This is achieved by increasing the bit life (minimizing premature bit wear), drilling faster that allows reducing drilling time, and also reducing tripping operations. The paper presents two parts. The first part compares the parametric physic-based swab and surge simulation results obtained from Bingham Plastic, Power Law, and Robertson and Stiff models. The aim is to show how the model predictions deviate from each other. Two 80-20 Oil Water ratios (OWR) and two 90-10 OWR oil-based drilling fluids with 1.96 sg and 2.0 sg were considered in vertical- and deviated wells. Simulation results analysis revealed that the deviations depend on the drilling fluid’s physical and rheological parameters as well as the well trajectory. Moreover, the model’s predictions were inconsistent. Data-driven machine learning (ML) modeling makes up the second section. Data-driven modeling was done using both software-generated datasets and field datasets. Results show that the Random Forest Regressor (RF), Artificial Neural Network (ANN), Long Short-Term Memory (LSTM), LightGBM, XGboost, and Multivariate regression models predict the training and test datasets with higher R-squared and minimum root means square values. Deploying the ML model in real-time applications and the planning phase would have the potential application of artificial intelligence for well planning and optimization processes.

Inflammation has a critical role in the development and progression of atherosclerosis. On the molecular level, inflammatory pathways negatively impact endothelial barrier properties and thus tissue homeostasis. Conformational changes and destruction of the glycocalyx further promote proinflammatory pathways also contributing to procoagulability and a prothrombotic state. In addition, changes in the extracellular matrix composition lead to (peri-)vascular remodelling and alterations of the vessel wall, e.g., aneurysm formation. Moreover, progressive fibrosis leads to reduced tissue perfusion due to loss of functional capillaries. The present review aims at discussing molecular and clinical effects of inflammatory processes on the micro- and microvas-culature with a focus on peripheral artery disease.

There is clinical concern about the combined use of alcohol and opiates. Several lines of evidence support an interaction between alcohol and the endogenous opioid system. Thus, we hypothesized that ethanol by causing the release of opioid peptides may sensitize the system to the action of exogenous opioids such as morphine. In this study, using the place conditioning paradigm as an animal model of reward, we determined whether a morphine challenge would alter the pre-established preference induced by ethanol conditioning in mice, and whether this response was mediated by the mu opioid receptor (MOP). Given that ethanol exposure stimulates the release of opioid peptides, we also assessed the role of beta-endorphin (β-END) and enkephalins (ENKs) in this response. Mice lacking MOP, β-END and/ or ENKs as well as their respective wild-type controls were tested for baseline place preference on day 1, conditioned with ethanol (2 g/kg) versus saline on days 2 to 4 and then tested under a drug-free state for postconditioning place preference on day 5. On day 8, mice received a single injection of morphine (5 mg/kg) and were tested for place preference. On each test day, mice were placed in the central neutral chamber and allowed to freely explore the conditioning chambers. The amount of time that mice spent in each chamber was recorded. We found that a challenge dose of morphine given on day 8 enhanced the CPP response in mice previously conditioned with ethanol. This response was abolished in MOP null mice, confirming the role of MOP in this response. Although this enhanced response was not altered in mice lacking either β-end or ENKs compared to their wild-type littermates/controls, it was completely blunted in mice lacking both β-end and enkephalins. Together, these results suggest that these opioid peptides jointly mediate the crosstalk between the rewarding actions of morphine and ethanol.

Iron oxide nanoparticles have been investigated due to their suitable characteristics for diverse applications in the fields of biomedicine, electronics, water/wastewater treatment and sensors. Maghemite, magnetite and hematite are the most widely studied iron oxide particles and have ferrimagnetic characteristics. When very small, however, these particles have superparamagnetic properties and are called superparamagnetic iron oxide nanoparticles (SPIONs). Several methods are used for the production of these particles, such as coprecipitation, thermal decomposition and microemulsion. However, the variables of the different types of synthesis must be assessed to achieve greater control over the particles produced. In some studies, it is possible to compare the influence of variations in the factors for production with each of these methods. Thus, researchers use different adaptations of synthesis based on each objective and type of application. With coprecipitation, it is possible to obtain smaller, more uniform particles with adjustments in temperature, pH and the types of reagents used in the process. With thermal decomposition, greater control is needed over time, temperature as well as the proportion of surfactants and organic and aqueous phases in order to produce smaller particles and a narrower size distribution. With the microemulsion process, the control of the confinement of the micelles formed during synthesis through the proportions of surfactant and oil makes the final particles smaller and less dispersed. These nanoparticles can be used as additives for the creation of new materials, such as magnetic bacterial cellulose, which has different innovative applications. Composites that have SPIONs, which are produced with greater rigour with regards to size and distribution, have superparamagnetic properties and can be used in medical applications, whereas materials containing larger particles have ferromagnetic applications. To arrive at a particular particle with specific characteristics, researchers must be attentive to both the mechanism selected and the production variables to ensure greater quality and control of the materials produced.

The tumor microenvironment of colorectal cancer (CRC) is heterogenous; thus, it is likely that multiple immune-related and inflammatory markers are simultaneously expressed in the tumor. The aim of this study was to identify immune-related and inflammatory markers expressed in freshly frozen CRC tissues and to investigate whether they are related to the clinicopathological features and prognosis of CRC. Seventy patients with CRC who underwent curative surgical resection between December 2014 and January 2017 were included in this study. Tissue samples were obtained from tumor and non-tumor areas in the patients’ colons. The concentrations of immune-related markers (APRIL/TNFSF13, BAFF, LAG-3, PD-1, PD-L1, and CTLA-4) and inflammatory markers (CHIT, MMP-3, osteocalcin, pentraxin-3, sTNF-R1, and sTNF-R2) in the samples were measured using the Bio-plex Multiplex Immunoassay system. The concentrations of APRIL/TNFSF13, BAFF, and MMP-3 in the samples were significantly high; thus, we conducted analyses based on the cutoff values for these three markers. The high APRIL/TNFSH13 expression group showed a significantly higher rate of metastatic lesions than the low expression group, whereas the high MMP-3 expression group had higher CEA levels, more lymph node metastases, and more advanced disease stages than the low expression group. The five-year disease-free survival of the high MMP-3 expression group was significantly shorter than that of the low expression group (65.1% vs. 90.2%, p=0.033). This study provides evidence that the APRIL/TNFSF13, BAFF, and MMP-3 pathway is overexpressed CRC tissues and is associated with unfavorable clinicopathological features and poor prognosis in CRC patients. These markers could serve as diagnostic or prognostic biomarkers for CRC.

We explored the trends and the diversity of the mortality and risk factors of age-standardized cardiovascular diseases (CVD) and cerebrovascular disease (CBD) from 2000 to 2020 in five advanced Asian countries, including China, Japan, Singapore, South Korea, and Taiwan. Data were obtained from the Global Burden of Disease (GBD) report. Overall, the CVD mortality rates decreased gradually in all five Asian countries. Singapore had the highest rates before 2011, but Taiwan replaced Singapore as the nation of the highest mortality rates after 2012. Additionally, South Korea had the highest CBD mortality rates before 2016, while Taiwan experienced a peak in 2011 and remained high thereafter. The absolute difference (AD) and relative difference (RD) for CVD mortality rates showed clear declining trends in all five countries, with South Korea and China displaying the highest reductions, respectively. It is noteworthy that overweight and obesity exhibited an increasing trend in all these five Asian countries, with China demonstrating the highest AD and RD. Except for Taiwan, where a negative association of r=-0.732 was observed, CVD mortality was positively associated with CBD, hypertension, and DM mortality in all other four countries, therefore, further investigation into these relationships are needed.

Antibiotic resistance has emerged as one of the major concerns to public human health due to issues in treatment and control of major infectious diseases. From discovery of penicillin in 1940, the antibiotic resistance originated and now developed the microorganisms as resistive strains to the major available antibiotics. Furthermore, studies on this captivating activity of the microbes provide insight into the complexities of microbial physiology and may offer some guidance in preventing the onset and consequent development of antibiotic resistance. Despite of initiatives taken in last few decades to overcome the issues, the trends of antibiotic resistance gained much peak. Antibiotic resistance appears to have emerged as a result of various factors including antibiotics being misused and overused in both the medical field and the agricultural sector. Important factors in the development of antibiotic resistance also include bacterial mutation, unplanned evolution, and horizontal gene transfers. In addition, antibiotics resistance imposed the financial consequences to public health and drug development research. Future advancements related to innovative antibiotics and molecular drug designing become challenge for researchers due to intensive multiple antibiotics resistance among human population. Many researches have been conducted on origin, evolutionary aspect of antibiotic resistance and mechanisms of antibiotics resistance, but its effect on future drug development is little understood. Therefore, the recent review will highlight the role of antibiotic resistance in drug designing and impacts of antibiotic resistance on drug development in future.

A considerable number of patients with angina or myocardial ischemia have no significant coronary artery disease on invasive angiography. In recent years, several steps towards a better comprehension of the pathophysiology of these conditions, angina or ischemia with non-obstructive coronary arteries (ANOCA/INOCA) have been made. Nevertheless, several gaps in knowledge still remain. This review is intended to provide a comprehensive overview of ANOCA and INOCA with a particular focus on pathophysiology, recent diagnostic innovations, gaps in knowledge and treatment modalities.

The aim of the study was to evaluate the impact of different rearing systems (organic, conventional and traditional) on the physicochemical properties and oxidation states of pork meat. Samples (M.biceps femoris) were obtained from producers directly 3 days after slaughtering. Chemical composition (dry matter, protein, collagen, fat, ash), colour (L*, a*, b*) and oxidation state (Thiobarbituric acid reactive substances -TBARS, free fatty acid -FFA and antioxidant capacity-2,2-diphenyl-1-picrylhydrazyl) of the meat samples were determined. The results indicated that the rearing system affects most of the evaluated parameters. The significant difference (P < 0.05) was observed in colour parameter L* and b*, where the conventional pork was darker and the organic pork yellower. Total protein content in meat of conventional pigs was higher, whereas the fat content in the meat of organically reared pigs was higher than the meat of pigs from both other rearing systems. Organic pork was more susceptible to oxidation process due to its higher TBARS and FFA values and lower antioxidant capacity, which may result in inferior technological properties of meat.

There exists a plethora of studies examining the psychological and physical impacts of COVID-19 on infected victims. Fewer studies have been published assessing the different types of impacts that an individual’s COVID-19 infection has on close friends and family members. This is the first scoping review to gauge the reported psychosocial issues and daily hassles that impact the relatives and friends of infected individuals. This study was conducted by inputting key terms/MeSH terms into selected internet databases to locate prospective studies. The frameworks of scoping reviews by Arksey et al. and the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) were utilized in the methodology for identifying and selecting the studies. After data extraction, 37 studies were deemed suitable for analysis. The findings generated from each study was placed into combined categories. A total of 16 combined categories were generated from the amalgamation of the findings. The results show that psychosocial feelings (e.g., anxiety, stress, depression) was the category with the highest prevalence of grouped findings. The results from this study may serve as the impetus for future interventions targeting the alleviation of psychosocial feelings or day to day hassles associated with having a loved one inflicted with a severe illness.

As the demand for liquefied petroleum gas (LPG) continues to increase worldwide, more LPG facilities are being constructed. However, the design and manufacture of pressure vessels used to store dangerous liquids or pressurised fluids, such as LPG, has been a major cause of hazards, including explosions and leakage. In this study, we aim to address this issue by designing and analysing a vertical pressure vessel with a capacity of 10 m3 of pressurised LPG in accordance with the American Society of Mechanical Engineers (ASME) code. Safety is the primary concern in designing pressure vessels due to the potential risks posed by accidents. Therefore, the main objective of this project is to design a pressure vessel that is safe from failure. The vessel is cylindrical with two elliptical heads, two nozzles, a manway and four leg supports, and we used Autodesk Inventor Professional 2023 for geometric modelling and Inventor Nastran for FEA analysis. To investigate the displacements, deflections and Von-Mises stresses in the pressure vessel, we used the finite element method, and Autodesk Inventor Nastran was employed for the analysis. Our results showed that changes to the structure of the pressure vessel were needed to reduce stress in the structure. Specifically, the displacement showed an inverse relationship with the tank section shell thickness. Moreover, we observed that the factor of safety increased linearly with shell thickness. We carefully considered permissible pressures and determined the required wall thickness to ensure acceptable maximum stresses. Our results indicate that the design is safe from failure, and the highest stresses are experienced by the manway followed by the shell, while the heads, nozzles and legs support experienced the lowest stresses. We also performed a theoretical calculation for the entire model and checked the results to ensure they were within the acceptable limits. In summary, our study highlights the importance of designing pressure vessels in accordance with ASME codes to ensure safety and prevent hazards caused by improper design and manufacturing. By using the finite element method, we identified potential stress points in the pressure vessel and were able to make modifications to improve its safety.

To reveal the relationship between metallodrugs and cancer malignancy, molecular networks of anti-cancer drugs were analyzed. Molecular networks in several types of cancers were analyzed in Ingenuity Pathway Analysis (IPA). Analysis of carboplatin revealed the causal network in diffuse large B-cell lymphoma. Analysis of 12 analyses of cisplatin treatment identified causal networks including camptothecin and NUPR1. The causal network of camptothecin, which includes PTEN, FAS, and IRF1, was inactivated in diffuse-type GC and activated in intestinal-type GC. Upstream regulator analysis of cisplatin revealed an increase in FAS, BTG2, SESN1 and CDKN1A, and the involvement of the tumor microenvironment pathway. Upstream regulators of cisplatin-treated lung adenocarcinoma included a histone deacetylase inhibitor, trichostatin A (TSA). Causal network of arsenic was inactivated in diffuse-type GC and activated in intestinal-type GC, and included ERK, EGFR, SRC, IKK and TP53. Prediction of RNA-RNA interactions with the causal network of arsenic identified 10 microRNAs including mir-101, mir-103, and mir-22. The results revealed the involvement of EMT in arsenic treatment. Analysis of oxaliplatin, a platinum drug, revealed that the SPINK1 pancreatic cancer pathway is inactivated in ischemic cardiomyopathy. The study showed the importance of the relationship between platinum drugs or arsenic trioxide and the tumor microenvironment in the treatment of resistant cancer in humans.

In today's complex and rapidly changing world, integrating values, critical thinking, and moral reasoning in language and literature education is crucial for equipping students with the skills to navigate multifaceted ethical dilemmas and become responsible global citizens. This article highlights the connection between language, literature, critical thinking, moral reasoning, and values integration in education. Through language and literature, teachers can incorporate values education in their classroom, which can benefit not only individual students but also society as a whole. Theoretical frameworks such as character education, moral education, and values education provide the basis for this approach. The researcher recommends strategies for incorporating values integration in language and literature curricula, including the use of literary texts that promote critical thinking and moral reasoning skills. The limitations of this approach, including the challenges in assessing the effectiveness of values integration and potential resistance from some stakeholders, are also discussed. Ultimately, this article aims to call on educators to take a proactive role in promoting values integration in language and literature education, guided by the principles of Jesus Christ and the belief that education is not only about acquiring knowledge but developing character as well. Moreover, this concept has a significant and lasting impact on students' overall development that highlights the importance of values and moral reasoning needed to live a better life.

Appropriate nutrition during pregnancy and the post-partum period is vital to both the parent and their offspring. Both under- and over-nourished status may have important microbial implications on the parental and infant gut microbiomes. Alterations to the microbiome can have implications for a person’s risk of obesity and metabolic diseases. In this review, we examine alterations in the parental gut, vaginal, placental, and milk microbiomes in the context of pre-pregnancy BMI, gestational weight gain, body composition, gestational diabetes, and parental diet. We also investigate how the infant gut microbiome may be altered by these different parameters. Many of the microbial changes seen in under- and over-nourished states in birthing parents may result in long-term implications to the health of offspring. Differences in diet appear to be a major driver of the parental, and subsequently milk and offspring microbiomes. Further prospective longitudinal cohort studies are needed examining nutrition and the microbiome to better understand its implications. Additionally, trials involving dietary interventions in child-bearing age adults should be explored to improve the parent and child’s risks for metabolic diseases.

Biogeochemical models estimate soil organic carbon (SOC) sequestration, crop growth, and yield. The DeNitrification and DeComposition (DNDC) model was used to simulate soil SOC dynamics and harvested C-biomass in rice-wheat rotation under organic/inorganic fertilizations with conventional tillage (CT) and reduced tillage (RT). Before calibration, DNDC under-predicted harvestable grain C-biomass of rice by 29.22% to 42.14% and over-simulated grain C-biomass of wheat by 55.01% with equal amounts of NPK and animal manure applied under CT. However, after calibration by adjusting default values of soil/crop parameters, DNDC simulated harvestable grain C-biomass of both crops very close to observed values (only -2.81% to -6.17% less). DNDC also predicted effects of nutrient management practices on grain C-biomass of rice/wheat under CT/RT using d-index (0.76 to 0.96) and the calculated root mean squared error (RMSE of 165.36 to 494.18 kg C ha-1). DNDC simulated SOC trends for rice-wheat using measured values of several statistical indices. Regression analysis between modeled and observed SOC dynamics was significant with R2 ranging from 0.35 to 0.46 (p < 0.01), and intercept ranging from 0.30 to 1.34 (p < 0.65). DNDC demonstrated that combined inorganic and organic fertilization may result in higher C-biomass and more SOC sequestration in rice-wheat systems.

Frontal incomes play an important role in human behavioral regulation and can be a determinant of eating behavior. The aim of this study was to analyse the frontal incomes in obese patients with and without Binge Eating Disorder (BED), compared to normoweight (NW) subjects and to analyse the effect of sex and binge disorder on quality of life, with age and BMI as covariates. A total of 114 participants comprised three different groups (obese with BED, obese without BED and NW) completed the Frontal Assessment Battery (FAB) and Impact of Weight on Quality of Life (IWQOL-lite). The results showed that obese patients, with and without BED, have poorer frontal functioning than NW persons. Obese patients with BED have lower performance in frontal income than obese patients without BED. Male participants have a higher perception of quality of life in all dimensions, with women showing lower values in self-esteem and sex life. Obese with BED show greater weaknesses in physical function. These results suggest that low frontal incomes and worse quality of life characterize obese patients, more evident in obese patients with BED.

Infectious diseases continue to be a significant threat to human health and civilization, and finding effective methods to combat them is crucial. In this research, we investigate the impact of awareness campaigns and optimal control techniques on infectious diseases without proper vaccines. Specifically, we develop an SIRS type mathematical model that incorporates awareness campaigns through media and treatment for disease transmission dynamics and control. The model displays two equilibria, a disease-free equilibrium and an endemic equilibrium, and exhibits Hopf bifurcation when the bifurcation parameter exceeds its critical value, causing a switch in the stability of the system. We also propose an optimal control problem that minimizes the cost of control measures while achieving a desired level of disease control. By applying the minimum principle to the optimal control problem, we obtain analytical and numerical results that show how the infection rate of the disease affects the stability of the system and how awareness campaigns and treatment can maintain system stability. This study highlights the importance of awareness campaigns in controlling infectious diseases and demonstrates the effectiveness of optimal control theory in achieving disease control with minimal cost.

Apiaceae plants have been widely used as traditional Chinese medicines (TCMs) for the treatment of removing dampness, relieving superficies, and dispelling cold, etc. In order to exploit the potential application and improve the yield and quality of Apiaceae medicinal plants (AMPs), The traditional use, phytochemistry, modern pharmacological use, effect of bolting and flowering (BF), and approaches for controlling the BF were summarized. Currently, about 228 AMPs have been recorded as TCMs with 6 medicinal parts, 72 traditional uses, 62 modern pharmacological uses, and 5 main kinds of metabolites. Three effect degrees (i.e., significantly affected, affected to some extent, and no significantly affected) could be classed based on the yield and quality. The BF of individual plants (e.g., Angelica sinensis) could be effectively controlled by the standard cultivation techniques, while the mechanism of BF has not been systemically revealed. This review will provide useful references for the reasonable exploration and high-quality production of AMPs.

Recently, the popularity of fermented foods has increased significantly. Lactofermented vegetable juice products are particularly important for our diet. The combination of the richness of the ingredients derived from vegetable juices and the products formed during lactic fermentation is a valuable source of nutrients. This study aimed to analyse the selected properties of fermented beetroot, carrot, and beetroot-carrot juices. The juices obtained from beetroot and carrot were fermented with bacterial strains Lactiplantibacillus plantarum or Levilactobacillus brevis. Density, extract, dry matter content, pH, total acidity, pigments (betalain or carotenoids), color, and LAB count were measured in the samples. The results obtained in this study showed that the used LAB strains were capable of fermenting the analyzed juices. It was proven that Lactiplantibacillus plantarum was a better strain for fermentation ion of vegetable juice. This might have been influenced by the fermentation temperature and the duration of the fermentation. However, this strain had a lower effect on carotenoids, which are water-insoluble pigments, than Lactiplantibacillus plantarum. The opposite observation was made for betalain, a water-soluble pigment with nitrogen in its structure.

Piroplasmosis, caused by Babesia spp. and Theileria spp., poses significant constraints for livestock production and upgradation in Bangladesh. Besides examining blood smears, few molecular reports are available from some selected areas in the country. Therefore, the actual scenario of piroplasmosis in Bangladesh is deficient. This study aimed to screen the piroplasms in different livestock species by molecular tools. A total of 276 blood samples were collected from cattle, gayals (Bos frontalis) and goats in five geographies of Bangladesh. Thereafter, screening was conducted through a polymerase chain reaction, and species were confirmed by sequencing. The prevalence of Babesia bigemina, B. bovis, B. naoakii, B. ovis, Theileria annulata and T. orientalis was 49.28%, 0.72%, 1.09%, 32.26%, 6.52% and 46.01%, respectively. The highest prevalence (79/109; 72.48%) of co-infections was observed with B. bigemina and T. orientalis. The phylogenetic analyses revealed that the sequences of B. bigemina, B. bovis, B. naoakii, B. ovis and T. annulata were included in one clade in the respective phylogram. In contrast, the sequences of T. orientalis were separated into two clades, corresponding to Type 5 and 7. To the best of our knowledge, this is the first molecular report on piroplasms in gayals and goats in Bangladesh.

Developing novel wound dressings containing medicinal plant extracts can have several potential benefits, including improving the therapeutic value of the dressings and reducing the cost of producing wound dressings. In this study, we prepared foam dressing containing Eclipta prostrata leaf extract and gelatin (Eclipta prostrata dressing). Chemical composition was verified using Fourier transform infrared spectroscopy (FTIR), and pore structure was obtained by scanning electron microscopy (SEM). The physical properties, including absorption and dehydration properties, were also evaluated. The chemical properties were measured to determine the pH environment after being submerged with Eclipta prostrata dressings. The results revealed that the Eclipta prostrata dressing had a pore structure with an appropriate pore size (313.25 ± 76.51 µm and 383.26 ± 64.45 µm for the Eclipta prostrata A and Eclipta prostrata B dressings, respectively). The Eclipta prostrata B dressing was more consistent porosity, resulting in a higher absorption capacity and faster dehydration rate. According to physical properties, the Eclipta prostrata B dressing is best used on low-exuding wounds. Furthermore, the Eclipta prostrata A and B dressings make a slightly acidic environment. Therefore, our foam dressing will not interfere wound healing process.

Adipocytes play a critical role in maintaining a healthy systemic metabolism by storing and releasing energy in the form of fat and helping to regulate glucose and lipid levels in the body. Adipogenesis is the process through which pre-adipocytes are differentiated into mature adipocytes. It is a complex process involving various transcription factors and signaling pathways. Dysregulation of adipogenesis has been implicated in the development of obesity and metabolic disorders. Therefore, understanding the mechanisms that regulate adipogenesis and the factors that contribute to its dysregulation may provide insights into the prevention and treatment of these conditions. RNA Binding Motif Single Stranded Interacting Protein 1 (RBMS1) is a protein that binds to RNA and plays a critical role in various cellular processes such as alternative splicing, mRNA stability, and translation. The RBMS1 polymorphism has been shown to be associated with obesity and Type 2 diabetes, but the role of RBMS1 in adipose metabolism and adipogenesis is not known. We show that RBMS1 is highly expressed during the early phase of differentiation of the murine adipocyte cell line 3T3-L1 and is significantly upregulated in adipose tissue depots and adipocytes of high-fat-fed mice, implying a possible role in adipogenesis and adipose metabolism. Knockdown of RBMS1 in pre-adipocytes impacted the differentiation process and reduced the expression of some of the key adipogenic markers. Transcriptomic and proteomic analysis indicated that RBMS1 depletion affected the expression of several genes involved in major metabolic processes, including carbohydrate and lipid metabolism. Our findings imply that RBMS1 plays an important role in adipocyte metabolism and may offer novel therapeutic opportunity for metabolic disorders such as obesity and type 2 diabetes.

China is a large agricultural country that produces a large amount of crop straw every year. Thus, the development of cost-effective and economic application of invasive plants is warranted. Biochars derived from crop straw has been proven to be promising for adsorbent materials. However, less studies have focused on biochar derived from different types of crop straw as adsorbent under the same conditions to compare their adsorption performance. Here, we characterized the five biochars in the same system (600 ℃). In results, GBC has higher ash content, pH, CEC, specific surface area, mineral composition and oxygen-containing functional groups. The adsorption kinetics can be explained adequately by pseudo-second-order model and Langmuir model, indicating that the adsorption behavior of the biochar is both physical adsorption and chemical adsorption, the adsorption process includes complexation reaction, cationic π bond, ion precipitation and electrostatic adsorption. In conclusion, GBC exhibited higher metal equilibrium adsorption capacities (125 mg·g-1 for Pb2+, 29 mg·g-1 for Cd2+). The solution pH, biochar dosing, pyrolysis temperature and the properties of these heavy metals were responsible for adsorption capacity, thus showing stronger affinity and better adsorption effect. Our results are important for the selection and utilization of plant-based biochar for different heavy metals.

Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception. This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat databases for papers and patents, respectively. Based on a total of 45 papers and 100 patent families up until 2021 reported worldwide, 11 technology categories were defined: six non-optical and five optical techniques. Every category was described for its characteristics while assessing its potential for application. Next, the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves, and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents. When comparing the reported technologies in papers to those in patents, scientific gaps were observed, as some of the patented technologies were not reported in the scientific literature, e.g., ion mobility and mass spectrometry approaches. Generally, more diverse approaches in all categories were found in patents, although they do require more scientific evidence through papers or industrial adoption to prove their robustness. Moreover, although there is a recent trend for non-invasive techniques, invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported (and might continue to be) in papers and patents. It was also observed that none of the technologies complies with all the industry requirements. Thus, more research and harmony between consumers, industry, and governments is necessary.

Plastic waste constitutes one of the most important sources of pollution worldwide. Despite the growing recycling trend, nowadays there are no effective technologies that can compensate for the continuous increase in plastic production. Polyesters and polyamides are one of the most produced single-use plastics, mainly used in manufacturing textiles and soft drinks bottles. Today, only a very low fraction of these polymers can be recycled. It can be done by exploiting two leading technologies: mechanical and chemical recycling. Mechanical recycling represents, nowadays, the most used industrial application. However, it can treat a very narrow range of waste materials due to the impossibility of removing dyes and the deterioration of mechanical properties due to the incompatibility of different plastic materials. Another critical limit of this recycling technology is the limited number of recycling loops that can be done due to the thermal degradation that occurs during the extrusion process. The second possibility is chemical recycling, which allows the depolymerization of the original product to recover the monomers directly. The main drawbacks are the long reaction times and the many solvents needed to achieve high-purity products. Therefore, chemical recycling is economically feasible, only for big companies that can produce the virgin polymer in situ. In this work, a new technology has been patented. This process is constituted of three main steps. The first one is the distillation-assisted cyclodepolymerization (DA-CDP), introduced as a modification of the CDP process. In this unit, cyclic oligomers together with high molecular weight compounds have been produced. Then, after polymer purification, it is possible to achieve the same molecular weight of the initial polymer in less than 30 minutes, exploiting the ring-opening polymerization (ROP) in the next step.

Backyard chickens are the most popular poultry in Bangladesh due to their production potential, and income generation. However, this farming can be a factor in zoonotic disease transmission. This study aimed to assess the knowledge, attitudes, and practices (KAP) related to biosecurity and management among backyard chicken households in Bangladesh. A cross-sectional survey was conducted among 295 households in six sub-districts of different six divisions of Bangladesh. The study used a convenient sampling technique to choose the divisions, then the districts and sub-districts. A well-structured pre-tested questionnaire was used to interview randomly selected households. In this study, most of the respondents relied on agriculture crop production (n=128) as their primary source of income, relied on scavenging for their birds’ feeding (34%), or fed their chickens whole or broken rice (32%), used tube well water (58%), used ash (38%) or sand (28%) as litter material. A significant proportion of households (40%) did not use any nest box at all. Results also showed that the most reported poultry diseases among backyard poultry farmers were Newcastle disease (49%), followed by coccidiosis (30%), fowl cholera (12%), and fowl pox (9%). In the multivariate logistic regression model, knowledge of biosecurity was associated with the household type (OR=1.4, p=0.04), knowledge of vaccination (OR=3.7, p=0.02), don’t know category (OR=4.2, p=<0.001), provision of the nest box (OR=9.5, p<0.001), training (OR=0.1, p=0.04), and air pollution (OR=0.1; p=0.04). The study found a huge knowledge gap on management and biosecurity practices present among backyard chicken farmers, which can negatively impact public health for instance in the outbreak of zoonotic diseases. Increasing farmers' biosecurity awareness through education and outreach activities could minimize the risk of disease transmission and improve both public and poultry health.

The design of torsional springs for Series Elastic Actuators (SEAs) is challenging, especially when it comes to balancing good stiffness characteristics and efficient torque robustness. This study focuses on the design of a lightweight, low-cost, and compact torsional spring for use in rotary series elastic actuator (ES-RSEA) of lumbar support exoskeleton. The exoskeleton is used as an assistive device to prevent lower back injuries. The torsion spring was designed following design for manufacturability (DFM) principles, focusing on minimal space and weight. The design process consisted of determining the potential topology and optimizing the selected topology parameters through finite element method (FEM) to reduce equivalent stress. The prototype was made using a waterjet cutting process with low-cost material (AISI-4140-alloy) and tested using a custom-made test rig. The results showed that the torsion spring had a linear torque-displacement relationship with 99% linearity, and the deviation between FEM simulation and experimental measurements was less than 2%. The torsion spring has a maximum torque capacity of 45.7 Nm and a stiffness of 440 Nm/rad. The proposed torsion spring is a promising option for lumbar support exoskeletons and similar applications requiring high stiffness, low weight-to-torque ratio, and cost-effectiveness.

Endophytic insect pathogenic fungi have a multifunctional lifestyle; in addition to its well-known function as biocontrol agents, it may also help plants respond to other biotic and abiotic stresses, such as iron (Fe) deficiency. This study explores M. brunneum EAMa 01/58-Su strain attributes for Fe acquisition. Firstly, direct attributes include siderophore exudation (in vitro assay) and Fe content in shoots and in the substrate (in vivo assay) were evaluated for three strains of Beauveria bassiana and Metarhizium bruneum. The M. brunneum EAMa 01/58-Su strain showed a great ability to exudate iron siderophores (58.4% surface siderophores exudation) and provided higher Fe content in both dry matter and substrate compared to the control and was therefore selected for further research to unravel the possible induction of Fe deficiency responses, Ferric Reductase Activity (FRA), and relative expression of Fe acquisition genes by qRT-PCR in melon and cucumber plants.. In addi-tion, root priming by M. brunneum EAMa 01/58-Su strain elicited Fe deficiency responses at transcriptional level. Our results show an early up-regulation (24, 48 or 72 h post inoculation) of the Fe acquisition genes FRO1, FRO2, IRT1, HA1, and FIT as well as the FRA. These results highlight the mechanisms involved in the Fe acquisition as mediated by IPF M. brunneum EAMa 01/58-Su strain.

Plastic contamination is a burning issue costing the global cotton and textile industries billions of dollars annually. Any time plastics from different sources end up in a cotton lint bale, the value to the textile mills plummets significantly. Various industry players have therefore made a concerted effort to find lasting solutions to the menace posed by plastic to cotton profitability and sustainability. Nevertheless, until now, there have been no up-to-date comprehensive documents detailing the numerous and ever-growing efforts committed to solving this challenge. Therefore, this article provides a detailed yet compact review of this highly dynamic subject matter. First, it puts into perspective plastic contamination in the cotton and textile industries. Then, the cotton value chain is subdivided into phases from pre-cultivation to textile mills. The root causes of plastic contamination are discussed in each stage, followed by discussions of some already developed and emerging solutions in response to the challenge by the affected industries and researchers. Concluding from the author’s perspective, the paper makes projections for the future directions of plastic mitigation efforts within the cotton and textile industries. This article also infers from the reviewed literature that research on finding alternative materials to plastic as module wrap, the development of new, effective, and all-condition plastic sensing techniques for ginning and spinning equipment, and standardized protocols for UAV in-field surveys of plastic trash are some of the areas that will be beneficial to finding a permanent solution to the challenge.

Onion is one of the most economically and nutritionally important vegetable crops in West Africa. However, high-yielding varieties that are accessible to farmers remain scarce. Onion lines from the World Vegetable Center’s Allium program were evaluated during the cool and dry season from September to March for three years to identify adapted onion lines suitable for sustainable intensi-fication of production systems in Northern Ghana and Southern Mali. Trials were implemented in the Upper East and Northern Regions of Ghana and in two districts in the Sudan savanna zones of Mali (Bougouni and Koutiala). Nine onion lines, including a check variety were assessed for yield stability using a randomized complete block design. The trials were carried out in “technology parks” under the joint management of farmers and researchers. Onion bulb weight was recorded for each plot after harvest. Separate analyses of variances were performed for each location and season. Analysis of variance of combined locations, seasons and lines was made to determine the most stable varieties using the line-superiority measure and ecovalence stability coefficients. Results indicated that lines AVON1310 and AVON1325 were most stable for yield performance over lo-cations and seasons. From the genotype main effects and genotype-by-environment interaction (GGE) biplot, the best performing lines were AVON1310 (33.32 t.h-1), AVON1308 (28.81 t.h-1) and AVON1325 (31.68 t.h-1). These lines are potential candidates for sustainable intensification of onion production in Ghana and Mali.

The life cycles and biodiversity of Pacific coast gastropods were analyzed by videomicroscopy and DNA barcoding of indi-viduals collected from tide pools and in plankton nets from a variety of shore stations. In many species (Families Calyptrae-idae, Cerithiopsidae, Strombidae, Vermetidae, Columbellidae, Nassariidae, Olivellidae, Hermaeidae, Onchidorididae, Gas-tropteridae, Haminoeidae), the free-swimming veligers were recovered from plankton collections; in Roperia poulsoni (family Muricidae) veligers were usually recovered from egg sacs where they had been retained although some escapees were found in plankton collections; in Pteropurpura festiva (family Muricidae) free-living veligers were also found; and in Atlanta californiensis (family Atlantidae) both veligers and adults were obtained from plankton collections making this a holoplank-tonic species. The results confirm that DNA barcoding using the COI barcode is a useful strategy to match life-cycle stages within species as well as to identify species and to document the level of biodiversity within the gastropods.

Background: The Davidson Airway Function &amp; Nasal Evaluation (DAFNE) Scoring System was developed as an intuitive and research-based scoring system that could be validated through beta testing and easily introduced to healthcare providers of several subspecialties who treat nasal obstruction and breathing disorders (MDs, PAs, PTs, APRNs, DDSs, and DCs). This scoring system was shown to increase the knowledge of airway function, nasal measurement parameters, and identification of proper treatment options for sleep and breathing disorders. The basis for the DAFNE score was developed from a systematic review of nasal measurement data. Methods: Electronic searches of PubMed, MEDLINE, EMBASE Cochrane Library, and Scopus of publications between 1988-2022 were used to identify studies validating nasal function measurement parameters to create the algorithm for the DAFNE Score™. The systematic review was accomplished using the 2020 ‘Preferred Reporting Items for Systematic Reviews’ (PRISMA) guidelines. Results: Twenty studies met the inclusion criteria for systematic review. Primary outcomes measurements demonstrated reliability, repeatability and validity of the DAFNE measurement technologies, data and output. Conclusions: The data analysis and systematic review uncovered a need and framework to develop and validate a web-based software algorithm for global access to improve the understanding of data interpretation of nasal measurements from three nasal measurement technologies. DAFNE Scoring should be used as an adjunct tool in routine clinical practice and research to further understand the technology data output and how to collaborate with other healthcare providers to improve patient outcomes.

Introduction: The purpose of this study was to determine the normative values of muscles’ contractile properties, lateral symmetry, and the impact of aging on muscle contractility in gymnastics. Materials and Methods: A survey of 81 athletes from different disciplines was undertaken: MAG (n = 26), WAG (n = 28), and RG (n = 27). The athletes’ average age was 15.41 ± 5.03 years. We try to establish the normative values for contraction time (muscle pairs) in gymnastics for Slovenian athletes for ten skeletal muscles. Results: Athletes’ age affects contractility in a negative way. Differences between the duration of contractility and age were found in all disciplines: m. BF (r = 0.48, p < 0.001), m. TB (r = 0.37, p < 0.013), m. ES (r = 0.17, p < 0.025), m. VL (r = 0.36, p < 0.00) and m. VM (r = 0.40, p < 0.000) at a statistical significance of p < 0.05. Conclusions: A comparison between the left and right sides shows little asymmetry in WAG and that the occurrence of injuries is in the middle range. In RG we see a bigger deviation, which could trigger the emergence of pain or injury in m. BF (13%) and m. GL (14.5%), but in MAG the deviation is greater in m. BB (12%), m. BF (11%) and m. GM (13%).

Adaption of laser communication terminals to airborne and lean-satellite platforms is now a vogue, made possible due to the progressing advancements in lightweight components and compactness of onboard electro-optical transceivers and control systems. This enables highly secured and superior data-transmission rates beyond multiple Gigabit/second on CubeSats and drones compared to Megabit/second rates offered by similar radio-transceivers form factors. However, laser-transmission links require a very stringent beam-pointing stability because they are easily perturbed by attitude variations and micro-vibrations generated by the host platform’s propulsion system or other mechanically active subsystems in proximity with the transmitter’s optical head. Severe line-of-sight jitter causes the downlink laser beam to drift from the targeted receiving system’s field-of-view, inducing pointing errors, increasing signal outage probability and information loss. We experimentally examine the platform jitter generated by the propellers of an hexacopter drone during ground operation and the attitude-control unit’s reaction wheels in a 6U CubeSat structure. We determined the vibration spectrum unique to these platforms and accordingly prescribe requirements for applicable optical fine pointing and disturbance isolation or suppression systems needed to achieve a high-fidelity laser-communication link.

Listeria ivanovii and L. monocytogenes, are the only pathogenic species of the genus Listeria and share many virulence factors and mechanisms of pathogenicity. L. ivanovii shows host tropism towards small ruminants and rodents and much lower virulence for humans compared to L. monocytogenes. However, severe infections caused by L. ivanovii, resulting in bacteremia, abortion and stillbirth, occasionally occurred in immunocompromised persons and in pregnant women, while in immunocompetent hosts L. ivanovii can cause gastroenteritis. In this review the updated knowledge on virulence aspects and distribution of L. ivanovii in the environment and in food are summarized. Recent research on its virulence characters at genome level gave indications on how pathogenicity evolved in this bacterial species. As for L. monocytogenes, L. ivanovii infections occur after the ingestion of contaminated food, so an overview of reports regarding its distribution in the food products was carried out to obtain indications on the categories of foods exposed to contamination by L. ivanovii. It was found that a wide variety of food products can be a source of this microorganism and that, like L. monocytogenes, L. ivanovii is able to persist in the food production environment. Studies on its ability to grow in enrichment and isolation media suggested that its occurrence in nature might be underestimated. Moreover, virulence varies among strains for differences in virulence character regulation, presence/absence of genetic regions and the possible instability of a Listeria pathogenicity genomic island, LIPI-2, which is unique to L. ivanovii. We can conclude that L. ivanovii, as a possible pathogen for animals and humans, requires more focused investigations regarding its occurrence in the environment and in food and on intra-species variability of pathogenic potential.

Volatile compounds, abundant in breath, can be used to accurately diagnose and monitor a range of medical conditions. This offers a non-invasive, low-cost approach with screening applications; however, uptake of this diagnostic approach has been limited by conflicting published outcomes. Most published reports rely on large scale screening of the public, at single time points and without reference to ambient air. Here, we present a novel approach to volatile sampling from cellular headspace and mouse breath that incorporates multi-time point analysis and ambient air subtraction revealing compound flux as an effective proxy of active metabolism. This approach to investigating breath volatiles offers a new avenue for disease biomarker discovery and diagnosis. Using gas chromatography mass spectrometry (GC/MS), we focus on low molecular weight, metabolic substrate/by-product compounds and demonstrate that this non-invasive technique is sensitive (reproducible at ~1 µg cellular protein, or ~500,000 cells) and capable of precisely determining cell type, status and treatment. Isolated cellular models represent components of larger mammalian systems and we show that stress- and pathology-indicative compounds are detectable in mice, supporting further investigation using this methodology as a tool to identify volatile targets in human patients.

With the rapid development of global offshore wind power, the demand for offshore wind power operation and maintenance is also increasing. Wisdomization of offshore wind farms is a practical need to improve the operation level and benefit of offshore wind farms. This paper first introduces the current development situation and characteristics of global offshore wind power, and expounds the current situation and main challenges of offshore wind power operation and maintenance market. Therefore, our paper discusses the innovation of offshore wind power operation and maintenance from the aspects of operation and maintenance management of offshore wind power, monitoring and analysis technology of units, far-reaching wind field monitoring and operation and maintenance risks. Then, combined with information technology and lean management concept, a smart operation and maintenance management platform for wind farms in far-reaching sea areas is built to explore centralized and intelligent operation and maintenance management mode, improve operation and maintenance efficiency of wind farms in far-reaching sea areas, and minimize operation and maintenance costs. Finally, through the research on the characteristics of 5G technology, combined with the practical experience of operation and maintenance, and in view of the characteristics of offshore wind farms, we analyze and propose several typical application scenarios of 5G technology in the intelligent operation and maintenance of offshore wind farms, which provides a new solution for the efficient operation and maintenance of offshore wind farms.

The sexual health needs of female immigrant adolescents in Canada have been largely unmet and have increased in magnitude over the last few years. Evidence suggests that immigrant adolescents lack sexual and reproductive health knowledge and use fewer sexual health-related services and sex education resources than do non-immigrant youth. In Pakistani immigrant adolescents, this difference appears to be associated with socio-cultural and religious practices. This paper aims to describe how first-or-second generation Pakistani-descent female adolescents, living in Canada, describe their perspectives on developing sexuality and well-being. In addition, this paper explicates female adolescents’ perceptions of their needs to support their sexuality while going through the adolescence stage. Using a qualitative interpretive descriptive design, individual interviews were conducted in combination with drawing timelines. A purposive sample of 21 female adolescents who were first- or second-generation Pakistani-descent was obtained. A thematic analysis approach was used for data analysis. Findings suggest that immigrant female adolescents encounter mental health concerns as a result of confusing messages they received from their parents related to sexuality. Also, discrimination, exclusion from sex education classes, and lack of knowledge on sexual health can result in social exclusion, avoidance of health care, and poor mental health outcomes such as depression and anxiety. These participants related experiences are potentially contributed by the lack of communication with parents about sexuality and lack of health care providers who can understand and speak to their needs and realities as immigrant individuals. Female adolescents expressed their need to break the silence around the topic of sexuality, to have a non-judgemental and blame-free attitude from adults, and for open, honest, and stigma-free conversations. This study provides preliminary qualitative evidence of the interplay between experiences of developing sexuality and overall well-being, particularly psychological wellbeing in female adolescent immigrants. It is crucial to involve, listen to, and incorporate adolescent girls’ voices when planning and implementing interventions to support healthy sexuality among immigrant adolescents.

We conducted a comparative study on the development of two synthetic methods. The solvent-free synthesis is a green chemistry method developed in a bid to ameliorate environmental adverse effects of the conventional solvent-based synthesis. The synthesis of novel dichloro and polymethoxy p-nitrophenylhydrazones through solvent free technique gave moderate to high yields which were however lower than those of the solvent-based method. The established solvent-free approach has several benefits, including universality and simplicity of the approach, catalyst-free conditions, non-use of an organic solvent, quick reaction time, fast and efficient workup, and un-solvated pure products in moderate to high yields.

A discharge mixing zone (DMZ) is a defined geographical area or volume of water in the receiving environment of a discharge where initial dilution of the effluent occurs and where exceedance of water quality criteria may be permitted. DMZs are essential to inform determination of discharge consent conditions and an important element of risk management frameworks to reduce any effects of the discharges on the environment and human health. In this review, we describe the principles and technical application of DMZs. We present an overview of the physical processes that govern the dispersion and dilution of wastewater discharges and the fate of contaminants in coastal environments and define key criteria for determining the size of DMZs. We summarize DMZ requirements in international legislation and guidance and exemplify their application to different types of discharges by means of case studies. The selected case studies illustrate different modelling tools for defining DMZs and different monitoring approaches to assess their effectiveness in achieving ecological and human health objectives.

Diarylethene is a prototypical molecular switch that can be reversibly photoisomerized between its open and closed forms. Ligands bpy-DAE-bpy, consisting of a phenyl-diarylethene-phenyl (DAE) central core and bipyridine (bpy) terminal substituents, are able to self-organize. They are investigated by scanning tunneling microscopy at the solid-liquid interface. Upon light irradiation, cooperative photochromic switching of the ligands is recognized down to the sub-molecular level. The closed isomers show different electron density of states (DOS) contrasts, attributed to the HOMO or LUMO molecular orbitals observed. More importantly, the LUMO images show remarkable differences between the open and closed isomers, attributed to combined topographic and electronic contrasts mainly on the DAE moieties. The electronic contrasts from multiple HOMO or LUMO distributions, combined with topographic distortion of the open or closed DAE, are interpreted by density functional theory (DFT) calculations.

In recent years, metabolomics has been used as a powerful tool to better understand the physiology of neurodegenerative diseases and identify potential biomarkers for progression. We used targeted and untargeted aqueous, and lipidomic profiles of the metabolome from human cerebrospinal fluid to build multivariate predictive models distinguishing patients with Alzheimer’s disease (AD), Parkinson’s disease (PD), and healthy age-matched controls. We emphasize several statistical challenges associated with metabolomic studies where the number of measured metabolites far exceeds sample size. We found strong separation in the metabolome between PD and controls, as well as between PD and AD, with weaker separation between AD and controls. Consistent with existing literature, we found alanine, kynurenine, tryptophan, and serine to be associated with PD classification against controls, while alanine, creatine, and long chain ceramides were associated with AD classification against controls. We conducted a univariate pathway analysis of untargeted and targeted metabolite profiles and find that vitamin E and urea cycle metabolism pathways are associated with PD, while the aspartate/asparagine and c21-steroid hormone biosynthesis pathways are associated with AD. We also found that the amount of metabolite missingness varied by phenotype, highlighting the importance of examining missing data in future metabolomic studies.

Heme is located in the active site of proteins and has diverse and important biological functions, such as electron transfer and oxygen transport and/or storage. The distortion of heme porphyrin is considered an important factor for the diverse functions of heme because it correlates with the physical properties of heme, such as oxygen affinity and redox potential. Therefore, clarification of the relationship between heme distortion and the protein environment is crucial in protein science. Here, we analyzed the fluctuation in heme distortion in the protein environment for hemoglobin and myoglobin using molecular dynamics (MD) simulations and quantum mechanical (QM) calculations. We also investigated the protein structures of hemoglobin and myoglobin stored in Protein Data Bank and found that heme is distorted along the doming mode, which correlates with its oxygen affinity, more prominently in the protein environment than in the isolated state, and the magnitude of distortion is different between hemoglobin and myoglobin. This tendency was also observed in the results of MD simulations and QM calculations. These results suggest that heme distortion is affected by its protein environment and fluctuates around its fitted conformation, leading to physical properties that are appropriate for protein functions.

The evolution of digital media in adolescents has changed the patterns and motives of use and the impact on their communication choices in their social and family networks. The objectives of this study are to understand how peers communicate adopting a social network (SN) or by voice and their social desirability. After the informant consent signature, the adolescents completed a series of self-report questionnaires on the use of SN, on communication preferences, and on social desirability through online. Most of the adolescents belonged to the 17-19 age group (83.6%) and were female (68.9%). Adolescents spent more than 3 hours/day on Whatsapp and more than 2 hours/day on Instagram, while the use of Facebook was on average only 35 minutes/day. Females used digital media for longer than males. Adolescents aged 17-19 years choose more Facebook and voice modes compared to adolescents aged 14 and 16 years. The alternative modes of Whatsapp and voice were chosen more than the social networks in their communication strategies, especially for negative topics. Motives for use were, in addition to boredom, related to maintaining one's social sphere with peers. Some educative considerations were made based on these results.

Magnetic resonance elastography (MRE) has revealed sexual dimorphism in brain stiffness in healthy individuals and multiple sclerosis (MS) patients. In the animal model of MS, experimental autoimmune encephalomyelitis (EAE), we showed previously that inflammation-induced brain softening was associated with alterations of the extracellular matrix (ECM). However, it remained unclear whether the brain ECM presents sex-specific properties that can be visualized by MRE. Therefore, we aimed here at quantifying sexual dimorphism in brain viscoelasticity in association with ECM changes in healthy and inflamed brains. Multifrequency MRE was applied to the midbrain of healthy and EAE mice of both sexes to quantitatively map regional stiffness. To define differences in brain ECM composition, gene expression of the key basement membrane components laminin (Lama4, Lama5), collagen (Col4a1, Col1a1) and fibronectin (Fn1) was investigated by RT-qPCR. We showed that the healthy male cortex expressed less Lama4, Lama5, Col4a1 but more Fn1 (all p < 0.05) than the healthy female cortex, which was associated with 9% softer properties (p = 0.044) in that region. At peak EAE, cortical softening was similar in both sexes compared to healthy tissue, with an 8% difference remaining between males and females during EAE (p < 0.001). Cortical Lama4, Lama5 and Col4a1 expression increased 2 to 3-fold in EAE in both sexes while Fn1 decreased only in males (all p < 0.05). No significant sex differences in stiffness were detected in other brain regions. In conclusion, sexual dimorphism in the ECM composition of cortical tissue in the mouse brain is reflected by in vivo stiffness measured with MRE and should be considered in future studies by sex-specific reference values.

This study provides a thorough review and analysis of the evolution of the Greek vehicle fleet over the last ~30 years, which is next used for the generation of high granularity fleet projections and for the estimation of relevant environmental benefits by 2030. The integrated methodology developed takes also into account vehicle clustering and the Brown’s Double Simple Exponential Smoothing technique that together with the adoption of COPERT based emission factors allow for the estimation of the anticipated emissions in 2030. Expected 2030 emissions levels suggest a reduction across all pollutants in comparison to 2018, ranging from 3.7% for PM10 to 54.5% for NMVOC (and 46% for CO, 14% for SO2, 28% for NOX and 21% for CO2). We find that Greece is on track with national goals concerning the reduction of air pollution from the transportation sector, stressing the positive contribution of EVs and new, "greener" vehicles, and setting new challenges for the further improvement of the sector beyond the 2030 outlook.

Abstract: Formation of DNA adducts is a key event for a genotoxic mode of action and its formation is often use as surrogate for mutation and cancer. Interest in DNA adducts are twofold, first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. High chemically specific mass spectrometry methods have been established for DNA adduct quantitation and elegant bio-analytic methods utilizing enzymes, various chemistries, and molecular biology methods to visualize the location of DNA adducts. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. We will briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technology that is expected to achieve both measures simultaneously. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.

Rotavirus (RV) and norovirus (NoV) are the leading cause of acute gastroenteritis (AGE) worldwide. Several studies have demonstrated that histo-blood group antigens (HBGAs) have a role in NoV and RV infections, since their presence on the gut epithelial surfaces is essential for the susceptibility to many NoV and RV genotypes. Polymorphisms in genes that code for enzymes required for HBGAs synthesis lead to secretor or non-secretor and Lewis positive and Lewis negative individuals. While secretor individuals appear to be more susceptible to RV infections, regarding NoVs infections there are too many discrepancies that prevent drawing conclusions. A second factor that influences enteric viral infections is the gut microbiota of the host. In vitro and animal studies have determined that the gut microbiota limits, but in some cases enhances, enteric viral infection. The ways microbiota can enhance NoV or RV infection include virion stabilization and promotion of virus attachment to host cells, whereas experiments with microbiota-depleted and germ-free animals point to immunoregulation as the mechanism by which the microbiota restricts infection. Human trials with live, attenuated RV vaccines and analysis of the microbiota in responders and non-responders individuals also allowed the identification of bacterial taxa linked to vaccine efficacy. As more information is gained on the complex relationships that are established between the host (glycobiology and immune system), the gut microbiota and the intestinal viruses, new avenues will be open for the development of novel anti-NoV and anti-RV therapies.

There is much concern about disruption of endocrine physiology regulated by steroid hormones in humans, other terrestrial vertebrates and fish by industrial chemicals, such as bisphenol A, and pesticides, such as DDT. These endocrine-disrupting chemicals influence steroid-mediated physiology in humans and other vertebrates by competing with steroids for receptor binding sites, disrupting diverse responses involved in reproduction, development and differentiation. Here I discuss that due to evolution of the progesterone receptor (PR) and mineralocorticoid receptor (MR) after ray-finned fish and terrestrial vertebrates diverged from a common ancestor, each receptor evolved to respond to different steroids in ray-finned fish and terrestrial vertebrates. In elephant shark, a cartilaginous fish, ancestral to ray-finned fish and terrestrial vertebrates, both progesterone and 17,20-beta-dihydroxy-progesterone activate the PR. During the evolution of ray-finned fish and terrestrial vertebrates, the PR in terrestrial vertebrates continued responding to progesterone and evolved to weakly respond to 17,20-beta-dihydroxy-progesterone. In contrast, the physiological progestin for the PR in zebrafish and other ray-finned fish is 17,20-beta-dihydroxy-progesterone, and ray-finned fish PR responds weakly to progesterone. The MR in fish and terrestrial vertebrates also diverged to have different responses to progesterone. Progesterone is a potent agonist for elephant shark MR, zebrafish MR and other fish MRs, in contrast to progesterone’s opposite activity as an antagonist for aldosterone, the physiological mineralocorticoid for human MR. These different physiological ligands for fish and terrestrial vertebrate PR and MR need to be considered in applying data for their disruption by chemicals in fish and terrestrial vertebrates to each other.

Gelatinization of starch content in pet foods can be impacted by several factors including moisture, retention time, and ingredients used. Starch gelatinization has been associated with digestibility but isn’t well studied using ingredients common in non-traditional canine diets. The objective of this research was to examine the impacts of dietary ingredient profile (traditional vs non-traditional) and assess impacts to total starch content and starch gelatinization. Traditional diets (n = 10) utilizing meat-based ingredients including chicken, chicken by-product meal, meat and bone meal and plant-based ingredients including rice, barley, oats, and corn were examined in comparison with non-traditional diets (n = 10) utilizing meat-based ingredients including alligator, buffalo, venison, kangaroo, squid, quail, rabbit, rabbit and salmon along with plant-based ingredients including tapioca, peas, chickpeas, lentils, potato, and pumpkin. Representative samples were collected via grab sample technique (5 samples/diet) and were assessed for total starch content as well as percent starch gelatinization. Difference between ingredient type was assessed using a Students t-test in SAS 9.4. Significance was set at P < 0.05. Distribution of total starch content based on ingredient type (traditional vs non-traditional) revealed that mean total starch content was higher in traditional diets as compared to non-traditional diets (P <0.0001). Conversely, starch gelatinization was found to be higher in non-traditional diets (P < 0.0001). Total starch content and total gelatinized starch had a strong negative correlation (P < 0.01) in traditional diets, though no correlation was observed in non-traditional diets. This negative correlation indicates a decrease in total gelatinized starch associated with increased total starch content. These novel data reveal important differences between starch content and gelatinization and could impact manufacturing processes for ingredient types as well as feeding recommendations. Unpredicted variation between ingredient formulations could potentially lead to decreased digestibility and absorption and may result in nutrient deficiencies.

Microtubule organizing centers (MTOCs) perform critical cellular tasks by nucleating, stabilizing and anchoring microtubule’s minus ends. These capacities impact tremendously a wide array of cellular functions ranging from ascribing cell shape to orchestrating cell division and generating motile structures, among others. The phylum Apicomplexa comprises over 6000 single-celled obligate intracellular parasitic species. Many of the apicomplexan are well known pathogens such as Toxoplasma gondii and the Plasmodium species, causative agents of toxoplasmosis and malaria, respectively. Microtubule organization in these parasites is critical for organizing the cortical cytoskeleton, enabling host cell penetration and the positioning of large organelles, drive cell division and direct the formation of flagella in sexual life stages. Apicomplexans are a prime example of MTOC diversity displaying multiple functional and structural MTOCs combinations within a single species. This diversity can only be fully understood in light of each organism's specific MT nucleation requirements and their evolutionary history. Insight into apicomplexan MTOCs had traditionally been limited to classical ultrastructural work by transmission electron microscopy. However, in the past few years, a large body of molecular insight has emerged. In this work we describe the latest insights into nuclear MTOC biology in two major human and animal disease causing Apicomplexans; Toxoplasma gondii and Plasmodium spp.

Syncope and presyncope occurring during work can affect safety and impair occupational performance. Few data are available regarding the prevalence of these events among workers. The possible role of sleep quality, mental stress and metabolic disorders in promoting syncope, presyncope and falls in workers is unknown. In the present study, 741 workers (male 35.4%; mean age 47±11 years), employed in different companies, underwent clinical evaluation and blood tests and completed questionnaires to assess sleep quality, occupational distress and mental disorders. The occurrence of syncope, presyncope and unexplained falls during their working life was assessed by an ad hoc interview. The prevalence of syncope, presyncope and falls of unknown origin was 13.9%, 27.0%, and 10.3%, respectively. The occurrence of syncope was associated with an increased risk of occupational distress (adjusted Odds Ratio aOR: 1.62, Confidence Intervals at 95%: 1.05-2.52), low sleep quality (aOR: 1.79 CI 95%: 1.16-2.77) and poor mental health (aOR: 2.43 CI 95%: 1.52-3.87). Presyncope was strongly associated with occupational distress (aOR: 1.77 CI 95%: 1.25-2.49), low sleep quality (aOR: 2.95 CI 95%: 2.08-4.18) and poor mental health (aOR: 2.61 CI 95%: 1.78-3.84), while no significant relationship was found between syncope or presyncope and metabolic syndrome. These results suggest that occupational health promotion interventions aimed at improving sleep quality, reducing stressors and increasing worker resilience might reduce syncope and presyncope events in the working population.

Cancer/testis (CT) antigens exhibit selective expression predominantly in immunoprivileged tissues in non-pathological contexts but are aberrantly expressed in diverse cancers. Because of their expression pattern, they have historically been attractive targets for immunotherapies. The investigation of mechanistic roles of CT antigens in promoting oncogenesis has historically been a prominent research question, and a growing number of studies implicate CT antigens in promoting almost all the hallmarks of cancer. This suggests that CT antigens may act as cancer drivers. CT antigens are expressed in head and neck squamous cell carcinomas, although their role in the pathogenesis, prognostication, and treatment for this family of cancers remains poorly studied. Given that CT antigens hold intriguing potential as therapeutic targets and as biomarkers for prognosis and therapeutic response and that they can provide novel insights into oncogenic mechanisms, their further study in the context of head and squamous cell carcinoma is warranted.

Several studies have demonstrated estrogen’s cardioprotective abilities in decreasing the fibrotic response of cardiac fibroblasts (CFs). However, the majority of these studies are not sex-specific, and those at the cellular level utilize tissue culture plastic, a substrate that has a stiffness much higher than physiological conditions. Understanding the intrinsic differences between male and female CFs under more physiologically “healthy” conditions will help to elucidate the divergences in their complex signaling networks. We aimed to do this by conducting sex-disaggregated analysis of changes in cellular morphology and relative concentrations of profibrotic signaling proteins in CFs cultured on 8kPa stiffness plates with and without 17-β estradiol (E2). Cyclic immunofluorescent analysis indicated that there is a negligible change in cellular morphology due to sex and E2 treatment and that the differences between male and female CFs are occurring at a biochemical rather than structural level. Several proteins corresponding to profibrotic activity had various sex-specific responses with and without E2 treatment. Single-cell correlation analysis exhibited varied protein-protein interaction across experimental conditions. These findings demonstrate the need for further research into the dimorphisms of male and female CFs to develop better tailored, sex-informed prevention and treatment interventions of cardiac fibrosis.

The use of flexible sensors has tripled over the last decade due to the increased demand in various fields including health monitoring, food packaging, electronic skins and soft robotics. Flexible sensors have the ability to be bent and stretched during use and can still maintain their electrical and mechanical properties. This gives them an advantage over rigid sensors that lose their sensitivity when subject to bending. Advancements in 3D printing have enabled the development of tailored flexible sensors. Various additive manufacturing methods are being used to develop these sensors including inkjet printing, aerosol jet printing, fused deposition modelling, direct ink writing, selective laser melting and others. Hydrogels have gained much attention in the literature due to their self-healing and shape transforming. Self-healing enables the sensor to recover from damages such as cracks and cuts incurred during use and this enables the sensor to have a longer operating life and stability. Various polymers are used as substrates on which the sensing conductive material is placed. Polymers including polydimethylsiloxane (PDMS), polyvinyl acetate (PVA), and Kapton are extensively used in flexible sensors. The most widely used nanomaterials in flexible sensors are carbon and silver, however, other nanomaterials such as iron, copper, manganese dioxide and gold are also used to provide controlled levels of conductivity or other functional properties.

Historical legacy of Eastern European and Balkans’ health systems was mutually interdependent and shaped by local socioeconomic circumstances. Three distinctive systems of risk sharing and health financing to develop since the late XIX century were the Bismarck, Beveridge, and Semashko systems. Modern day healthcare systems in these countries are challenged by population ageing, accelerated innovation in medical technology, growing purchasing power and rising demand for healthcare services. Supply side changes contribute to demand side efficiency bottlenecks in financing, driving the costs of the already expensive medical care up. All of the nations have a large share of citizens experiencing difficulty with affordability and access to medical care, particularly in rural and remote areas. Network of Health technology assessment agencies have mushroomed over the past three decades. Principles of health economics theory and cost-effective resource allocation are slowly gaining ground in governing authorities’ mindset and decision-making process. For many years to come, pharmaceuticals and medical services will remain dependent on out-of-pocket spending. Currently accelerating and spreading 4.0 Industrial Revolution, together with the Belt and Road Initiative, are likely to substantially impact the further economic development of this vast region. Post-Pandemic ‘Green’ Recovery strategies adopted by many of the Eastern European governments shall also make this transition towards sustainable development more difficult and challenging given the large dependency of all these economies upon traditional carbon fuels.

Flexible implementations of Monte Carlo Tree Search (MCTS), combined with domain specific knowledge and hybridization with other search algorithms, can be a very powerful for the solution of problems in complex planning. We introduce mctreesearch4j, a standard MCTS implementation written as a standard JVM library following key design principles of object oriented programming. We define key class abstractions allowing the MCTS library to flexibly adapt to any well defined Markov Decision Process or turn-based adversarial game. Furthermore, our library is designed to be modular and extensible, utilizing class inheritance and generic typing to standardize custom algorithm definitions. We demon- strate that the design of the MCTS implementation provides ease of adaptation for unique heuristics and customization across varying Markov Decision Process (MDP) domains. In addition, the implementation is reasonably performant and accurate for standard MDP’s. In addition, via the implementation of mctreesearch4j, the nuances of different types of MCTS algorithms are discussed.

Stumbling during gait is commonly encountered in patients who suffer from mild to serious walking problems, e.g. after stroke, in osteoarthritis, or amputees using a lower leg prosthesis. Instead of self-reporting, an objective assessment of the amount of stumbles in daily life would inform clinicians more accurately and enable the evaluation of treatments that aim to achive a safer walking pattern. An easy to use wearable might fullfill this need. The goal of the present study was to investigate whether a single inertial measurement unit (IMU) placed at the shank and machine learning algorithms could be used to detect and classify stumbling events in a dataset comprising of a wide variety of daily movements. Ten healthy test subjects were deliberately tripped by an unexpected and unseen obstacle while walking on a treadmill. The subjects stumbled a total of 276 times, both using an elevating recovery strategy and a lowering recovery strategy. Subjects also performed multiple Activities of Daily Living. During data processing, an event-defined window segmentation technique was used to trace high peaks in acceleration which could potentially be stumbles. In the reduced dataset, time windows were labelled with the aid of video annotation. Subsequently, discriminative features were extracted and fed to train seven different types of machine learning algorithms. Trained machine learning algorithms were validated using leave-one-subject-out cross-validation. Support Vector Machine (SVM) algorithms were most succesful, and could detect and classify stumbles with 100% sensitivity, 100% specificity and, 96.7% accuracy, in the independent testing dataset. The SVM algorithms were implemented in a user-friendly, freely available, stumble detection app named Stumblemeter. This work shows that stumble detection and classification based on SVMs is accurate and ready to apply in clinical practise.

Family Phyllanthaceae is one of the largest segregates of the eudicot order Malpighiales and its species are herb, shrub, and tree, which are mostly distributed in tropical regions. Certain taxonomic discrepancies exist at genus and family level. Here, we report chloroplast genomes of three Phyllanthaceae species—Phyllanthus emblica, Flueggea virosa, and Leptopus cordifolius— and compare them with six others previously reported Phyllanthaceae chloroplast genomes. The species of Phyllanthaceae displayed quadripartite structure, comprising inverted repeat regions (IRa and IRb) that separate large single copy (LSC) and small single copy (SSC) regions. The length of complete chloroplast genome ranged from 154,707 bp to 161,093 bp; LSC from 83,627 bp to 89,932 bp; IRs from 23,921 bp to 27,128 bp; and SSC from 17,424 bp to 19,441 bp. Chloroplast genomes contained 111 to 112 unique genes, including 77 to 78 protein-coding, 30 transfer RNA (tRNA), and 4 ribosomal RNA (rRNA) that showed similarities in arrangement. The number of protein-coding genes varied due to deletion/pseudogenization of rps16 genes in Baccaurea ramiflora and Leptopus cordifolius. High variability was seen in number of oligonucleotide repeats while analysis of guanine-cytosine (GC) content, codon usage, amino acid frequency, simple sequence repeats analysis, synonymous and non-synonymous substitutions, and transition and transversion substitutions showed similarities in all Phyllanthaceae species. We detected a higher number of transition substitutions in the coding sequences than non-coding sequences. Moreover, the high number of transition substitutions was determined among the distantly related species in comparison to closely related species. Phylogenetic analysis shows the polyphyletic nature of the genus Phyllanthus which requires further verification. We also determined suitable polymorphic coding genes, including rpl22, ycf1, matK, ndhF, and rps15 which may be helpful for the reconstruction of the high-resolution phylogenetic tree of the family Phyllanthaceae using a large number of species in the future. Overall, the current study provides insight into chloroplast genome evolution in Phyllanthaceae.

We start with the discussion on misapplication of classical probability theory by Feynman in his analysis of the two slit experiment (by following the critical argumentation of Koopman, Ballentine, and the author of this paper). The seed of Feynman's conclusion on the impossibility to apply the classical probabilistic description for the two slit experiment is treatment of conditional probabilities corresponding to different experimental contexts as unconditional ones. Then we move to the Bell type inequalities. Bell applied classical probability theory in the same manner as Feynman and, as can be expected, he also obtained the impossibility statement. In contrast to Feynman, he formulated his no-go statement not in the probabilistic terms, but by appealing to nonlocality. This note can be considered as a part of the author's attempts for getting rid off nonlocality from quantum physics.

All over the world energy is used for different purposes and hence its continuous high demand which has brought about an increase in crisis and prices of energy. Ghana has faced a lot of supply and high electricity consumption challenges over a period of time. The Energy Commission of Ghana has developed regulations and guidelines to help reduce high consumption challenges among users, these included the replacement of incandescent bulbs with fluorescent bulbs, ban of importation of low energy efficient appliances. In spite of the effort to reduce electricity wastage, there is still a high increase in electricity consumption. The research investigated what contributed to electricity consumption in Kwame Nkrumah University of Science and Technology with the lecture halls as the main focus, the research also analyzed the current occupant behavior characterized by the electrical energy consumption practices. And investigated how the contemporary theories for reducing energy consumption was used in the lecture halls. A questionnaire survey was conducted to investigate occupants on their energy use practices in lecture halls that causes wastages, observation was made to establish relevant data on the use of contemporary theories for energy reduction in lecture halls. In a total of 110 occupants that responded to the questionnaire, 79 occupants almost always turn off electrical fitting and fixtures when not in use. From the responses, a majority of the occupants claimed to comply to best practices of energy use. The research concluded that some contemporary theories to reduce energy consumptions was not used and considered in the lecture halls.

Mitochondria are essential to cell homeostasis, and alterations in mitochondrial distribution, segregation or turnover have been linked to complex pathologies such as neurodegenerative diseases or cancer. Understanding how these functions are coordinated in specific cell types is a major challenge to discover how mitochondria globally shape cell functionality. In this review, we will first describe how mitochondrial transport and dynamics are regulated throughout the cell cycle in yeast and in mammals. Second, we will explore the functional consequences of mitochondrial transport and partitioning on cell proliferation, fate acquisition, stemness, and on the way cells adapt their metabolism. Last, we will focus on how mitochondrial clearance programs represent a further layer of complexity for cell differentiation, or in the maintenance of stemness. Defining how mitochondrial transport, dynamics and clearance are mutually orchestrated in specific cell types may help our understanding of how cells can transition from a physiological to a pathological state.

The significance of diversity, composition, and functional attributes of the gut microbiota is recognized in human health and disease. Studies have also shown that the gut microbiota is related to human aging, and a causal relationship between gut microflora dysbiosis and chronic age-related disorders is also becoming apparent. Further, emerging evidence indicates that age-associated changes in the gut microbiome are predictors of human survival and longevity. Recent advances in our understanding of the cellular and molecular aspects of biological aging have revealed a cellular senescence-centric view of the aging process. However, the association between gut microbiome and cellular senescence is only beginning to be understood. The present review provides an integrative view of the emerging relationship between the gut microbiome and cellular senescence in aging and disease. Evidence relating to microbiome-mediated modulation of senescent cells, as well as senescent cells-mediated changes in intestinal homeostasis have been discussed. Unanswered questions and future research directions have also been deliberated to truly ascertain the relationship of the gut microbiome and cellular senescence for developing microbiome-based age-delaying and longevity promoting therapies.

In clinical guidelines, such as National Comprehensive Cancer Network guidelines, it is embodied as first therapeutic option the combination of erlotinib and bevacizumab, based on a small clinical trial which included patients with HLRCC. With this case report we bring out another treatment option, not described before, with extraordinary results based on the combination of a check-point inhibitor and a tyrosine-kinase inhibitor, based on theoretical tumor immunogenic environment.

Determining the DNA sequencing of a small element in the mitochondrial DNA (DNA barcoding) makes it possible to easily identify individuals of different larval stages of marine crustaceans without the need for laboratory rearing. It can also be used to construct taxonomic trees, although it is not yet clear to what extent this barcode-based taxonomy reflects more traditional morphological or molecular taxonomy. Collections of zooplankton were made using conventional plankton nets in Newport Bay and the Pacific Ocean near Newport Beach, California (Lat. 33.628342, Long. -117.927933) between May 2013 and January 2020, and individual crustacean specimens were documented by videomicroscopy. Adult crustaceans were collected from solid substrates in the same areas. Specimens were preserved in ethanol and sent to the Canadian Centre for DNA Barcoding at the University of Guelph, Ontario, Canada for sequencing of the COI DNA barcode. From 1042 specimens, 544 COI sequences were obtained falling into 199 Barcode Identification Numbers (BINs), of which 76 correspond to recognized species. The results show the utility of DNA barcoding for matching life-cycle stages as well as for documenting the diversity of this group of organisms.

In this note, I analyze the data generated by M. Fodje's (2013) simulation programs "epr-simple" and "epr-clocked". They were written in Python and published on Github. Inspection of the program descriptions shows that they make use of the detection-loophole and the coincidence-loophole respectively. I evaluate them with appropriate modified Bell-CHSH type inequalities: the Larsson detection-loophole adjusted CHSH, and the Larsson-Gill coincidence-loophole adjusted CHSH (NB: its correctness is conjecture, we do not have proof). The experimental efficiencies turn out to be approximately eta = 81% (close to optimal) and gamma = 55% (far from optimal). The observed values of CHSH are, as they should be, within the appropriately adjusted bounds. Fodjes' detection-loophole model turns out to be very, very close to Pearle's famous 1970 model, so the efficiency is close to optimal. The model has the same defect as Pearle's: the joint detection rates exhibit signaling. Fodje's coincidence-loophole model is actually a clever modification of his detection-loophole model. Because of this, however, it cannot lead to optimal efficiency.

SLI and SLD constitute two autonomous neurodevelopmental disorders, which frequently cause challenges in the diagnosis process, especially due to their nature, which has caused disagreement among clinicians regarding their recognition as separate or overlapping disorders. The objective of the study was to enlighten the path of valid diagnosis and intervention during adolescence when the two disorders change their manifestation and overlap. 200 Greek adolescents (140 boys and 60 girls), 124 already diagnosed with SLD and 76 diagnosed with SLI, 12-16 years old, participated in the study. All participants were assessed in reading, oral and written language and mathematics (mathematical operations and mathematical reasoning) along with IQ testing. In order to determine statistically significant differences, the chi-square test, independent samples t-test, odd ratios and their 95 per cent confidence intervals were implemented. The results revealed that the SLI group presented significantly greater difficulties than SLD in their overall cognitive-mental profile and in most language and mathematical measurements (number concept, executive-procedural part of solving operations and mathematical reasoning). The similarity of the two groups was mainly detected in their deficient metacognitive, metalinguistic and metamnemonic strategies. The research concludes that SLD adolescents managed to overcome their difficulties to a significant degree, while adolescents with SLI still struggle with many learning areas.

The physiological response to a psychological stressor broadly impacts energy metabolism. In-versely, changes in energy availability affect the physiological response to the stressor in terms of hypothalamus, pituitary adrenal axis (HPA) and sympathetic nervous system activation. Glu-cocorticoids, the endpoint of the HPA axis, are critical checkpoints in endocrine control of ener-gy homeostasis and have been linked to metabolic diseases including obesity, insulin resistance and type 2 diabetes. Glucocorticoids, through the glucocorticoid receptor, activate transcription of genes associated with glucose and lipid regulatory pathways and thereby control both physi-ological and pathophysiological systemic energy homeostasis. Here, we summarize the current knowledge of glucocorticoid functions in energy metabolism and systemic metabolic dysfunc-tion, particularly focusing on glucose and lipid metabolism. There are elements in the external environment that induce lifelong changes in the HPA axis stress response and glucocorticoid levels, the most prominent are early-life adversity, or exposure to traumatic stress. We hypothe-sise that when the HPA axis is so disturbed after early-life adversity, it will fundamentally alter hepatic gluconeogenesis, inducing hyperglycaemia, and hence crystalise the significant lifelong risk of developing either the metabolic syndrome, or type 2 diabetes. This gives a “Jekyll and Hyde” role to gluconeogenesis, providing the necessary energy in situations of acute stress, but driving towards pathophysiological consequences when the HPA axis has been altered.

The rapid development of information and communication technologies has led to the use of new and digital technologies in education which involves combinations of text, graphics, audio, video, animations and other eLearning resources such as authoring tools, Learning Management System (LMS), Mobile learning and others. Arguably, using LMS leaves much to be desired. The inherent problem here is that the future of extensive adoption of ICT via LMS to enhance and promote classroom interaction in Open and Distance Learning (ODL) is bleak. This is worrisome given that the country is lagging far behind in the innovative use of this web 2.0 technology to impart knowledge. Further, the low-level application of LMS in instruction connotes the loss of inherent advantages in its adoption. Also, the online setting which makes students less nervous and interactive, sharing of ideas and viewpoints; and a host of other benefit will be lost. While evidence has shown that LMS is not a new phenomenon, the use of LMS in ODL is still at its infancy, particularly in Nigeria. Research in this area is rare. A quick search on prominent research databases could testify that. It is on this thrust that this study investigates University of Ibadan undergraduate students’ perceived roles and readiness towards integration of learning management system into teaching and learning.

The -blocker (S)-practolol ((S)-N-(4-(2-hydroxy (isopropylamino)propoxy)phenyl)acetamide) was synthesized with 96% enantiomeric excess (ee) from the chlorohydrin (R)-N-(4-(3-chloro-2 hydroxypropoxy)phenyl)acetamide, which was produced in 97% ee and with 27% yield. Racemic building block 1-((1H-indol-4-yl)oxy)-3-chloropropan-2-ol for the -blocker pindolol was produced in 53% yield and (R)-1-((1H-indol-4-yl)oxy)-3-chloropropan-2-ol was produced in 92% ee. The chlorohydrin 7-(3-chloro-2-hydroxypropoxy)-3,4-dihydroquinolin-2(1H)-one, a building block for a derivative of carteolol was produced in 77% yield. (R)-7-(3-chloro-2-hydroxypropoxy)-3,4-dihydroquinolin-2(1H)-one was obtained in 96% ee. The S-enantiomer of this carteolol derivative was produced in 97% ee in 87% yield. Racemic building block 5-(3-chloro-2-hydroxypropoxy)- 3,4-dihydroquinolin-2(1H)-one, building block for the drug carteolol was also produced in 53% yield, with 99% ee of the R-chlorohydrin (R)-5-(3-chloro-2-hydroxypropoxy)-3,4-dihydroquinolin-2(1H)-one. The yield of all four chlorohydrins increased by use of catalytic amounts of base. The reason for this was found to be less formation of the dimeric by-products compared to use of higher concentration of the base. An overall reduction of reagents and reaction time was also obtained compared to our previous reported data of similar compounds. The enantiomers of the chlorohydrin building blocks were obtained by kinetic resolution of the racemate in transesterification reactions catalyzed by Candida antarctica Lipase B (CALB) from SyncoZymes Co, Shanghai, China. Optical rotations confirmed the absolute configuration of the enantiopure drugs.

Abstract: Bacillus subtilis fmb60, which has broad-spectrum antimicrobial activities was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D–F (1-3) and two amicoumacin derivatives, amicoumacins D, E (4, 5), were identified, and are reported here for the first time. Lipoamides D–F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 µg/mL. Amicoumacin E scavenged 38.8% of ABTS+ radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and ace gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of B. subtilis fmb60, and genome mining for clusters of secondary metabolites of B. subtilis fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.

This study examines the improvement in coupled intercomparison project phase six (CMIP6) models against the predecessor CMIP5 in simulating mean and extreme precipitation over the East Africa region. The study compares the climatology of the precipitation indices simulated by the CMIP models with the CHIRPS dataset using robust statistical techniques for 1981 – 2005. The results display the varying performance of the general circulation models (GCMs) in the simulation of annual and seasonal precipitation climatology over the study domain. CMIP6-MME shows improved performance in the local annual mean cycle simulation with a better representation of two peaks, especially the MAM rainfall relative to its predecessor. Moreover, simulation of extreme indices is well captured in CMIP6 models relative to its predecessor. The CMIP6-MME performed better than the CMIP5-MME with lesser biases in simulating SDII, CDD, and R20mm over East Africa. Remarkably, most CMIP6 models are unable to simulate extremely wet days (R95p). A few CMIP6 models (e.g., NorESM2-MM and CNRM-CM6-1) depicts robust performance in reproducing the observed indices across all analyses. Conversely, OND season shows the overestimation of some indices (i.e., R95p, PRCPTOT), except for SDII, CDD, and R20mm. Consistent with other studies, the mean ensemble performance for both CMIP5/6 shows better performance due to the cancellation of some systematic errors in the individual models. Generally, the CMIP6 depicts improved performance in the simulation of MAM season akin CMIP5 models. However, the new model generation is still marred with uncertainty, thereby depicting substandard performance over the East Africa domain. This calls for further investigation of attribution studies into the sources of persistent systematic biases and a prerequisite for identifying individual models with robust features that can accurately simulate observed patterns for future usage.

Ischemic stroke is one of the most disabling diseases and a leading cause of death globally. Despite advances in medical care, the global burden of stroke continues to grow, as no effective treatments to limit or reverse ischemic injury to the brain are available. However, recent preclinical findings have revealed the potential role of transient receptor potential cation 6 (TRPC6) channels as endogenous protectors of neuronal tissue. Activating TRPC6 in various cerebral ischemia models has been found to prevent neuronal death, whereas blocking TRPC6 enhances sensitivity to ischemia. Evidence has shown that Ca2+ influx through TRPC6 activates cAMP response element-binding protein (CREB), an important transcription factor linked to neuronal survival. Additionally, TRPC6 activation may counter excitotoxic damage resulting from glutamate release by attenuating the activity of NMDA receptors of neurons by posttranslational means. Unresolved though, are the roles of TRPC6 channels in non-neuronal cells such as astrocytes and endothelial cells. Moreover, TRPC6 channels may have detrimental effects on the blood-brain barrier, although their exact role in neurovascular coupling requires further investigation. This review discusses evidence-based cell-specific aspects of TRPC6 in the brain to assess the potential targets for ischemic stroke management.