Supplements in fish nutrition can help alleviate pressure on the wild stock; however, there is limited information on the benefits of rooibos tea extract in aquafeed. This study aimed to determine the effect of rooibos tea extract inclusion in the commercial diet on the growth performance of larval fish. Fish were reared on three diets: a commercial diet with 30% fermented or green rooibos extract and a commercial tilapia diet (control). Fish (initial weight of 0.54 ± 0.008 g and length of 2.22 ± 0.01 cm) were randomly distributed into recirculating aquaculture systems in triplicates at 100 fish per tank. Fish was hand-fed thrice daily for eight weeks at 2% body weight. After that, samples were withdrawn to measure growth parameters. Fish-fed fermented, and green tea extracts exhibited a significantly (p ˂ 0.05) higher weight gain than the controls. The feed conversion ratio was higher in the control group, fermented (1.50 ± 0.25 cm) and green tea (1.41 ± 0.07 cm). Similarly, the control group exhibited the highest condition factor (K=13.14 ± 4.87). The two treatment groups were comparable (p >0.05) in growth performance. Overall, the evidence indicates that adding rooibos tea extracts to the basal diet can improve fish growth.

Backgrounds Linear association has widely been assumed for prediction of aging-related fractional anisotropy (FA) decline in white matter of the brain. While useful for testing significance of the aging effect, it fails to identify a threshold age before and after which the age-FA association changes. Identification of such a threshold is often of clinical interest for timely intervention. Methods We employed a sigmoidal growth function to test a threshold effect in age triggering onset of cerebral decline in 21 white matter tracts, and compared its fitting performance to those of linear, and power regression. The study sample was a normal healthy cohort of 106 participants with ages in mid-life ranging from 18 to 60 years. Results Of the 21 white matter tracts analyzed, the posterior thalamic radiation showed better fit with sigmoidal curve model, compared to a linear or power regression. The estimated threshold age in years (95% confidence interval) were 47.2 (44.1-48.4). Conclusion While available evidence regarding the presence of a specific age threshold for cerebral decline in mid-life based on FA was limited, the posterior thalamic radiation exhibited a threshold age of 47.2. Beyond this age point, we observed a significant change in the FA risk pattern.

This study investigates the impact of Foreign Direct Investment (FDI) on economic growth and examines the causality between FDI and economic growth in Bangladesh during 1972-2013. Gross Domestic Product (GDP), export performance (EXP), Foreign Direct Investment (FDI), and Gross Fixed Capital Formation (GFCF) are considered to capture the objective of the study. The study methodology includes some systematic steps. As the data used in the study is time-series in nature, the author employs unit root tests, and in this case, Augmented Dickey-Fuller (ADF) and Phillips-Perron (PP) tests are used. Then Johansen’s cointegration test, Granger causality test, regression with Newey-West Standard Error and Vector Error Correction Model (VECM) are applied. By using the ADF and PP test the study reveals that the variables of four-time series are integrated of I (1) i.e. they are stationary at first difference. Regression analysis result demonstrates that FDI has a positive effect on economic growth. The Granger Causality test discloses that there is a unidirectional relationship between FDI and economic growth. But the VECM estimation finds that in the long run FDI negatively affects economic growth.

Both tissue repair and regeneration are a priority in regenerative medicine. Retinitis pigmentosa (RP), a complex retinal disease characterized by the progressive loss of impaired photoreceptors, is currently lacking effective therapies: this represents one of the greatest challenge in the ophthalmological research field. Although this inherited retinal dystrophie is still incurable genetic disease, the oxidative damage is an important pathogenetic element that may represent a viable target of therapy. In this review, we summarize the current neuroscientific evidences about the cell therapies effectiveness, especially based on mesenchymal cells, in RP and focus on their therapeutic actions: limitation of both oxidative stress and apoptotic processes triggered by the disease, promoting cell survival. Cell therapy could therefore represent a feasible therapeutic option in RP.

Bio-based glass-forming materials are now considered for thermal energy storage in building applications. Among them, Xylitol appears as a biosourced seasonal thermal energy storage material with high potential. It has a high energy density, a high and stable undercooling allowing storing solar energy at ambient temperature thus, reducing thermal losses and the risk of spontaneous nucleation (i.e., the risk of losing the stored energy). Generally when the energy is needed, the discharge triggering of the storage system is very difficult as well as reaching a sufficient power delivery. Both are indeed the mains locks for the use of pure Xylitol in seasonal energy storage. Different techniques have been hence considered to crystallize highly undercooled Xylitol. Nucleation triggering of highly undercooled pure Xylitol by using an air lift reactor has been proven here. This method should allow reaching performances matching with building applications (i.e., at medium temperatures, below 100 °C). The advantages of this technique compared to other existing techniques to activate the crystallization are discussed. The mechanisms triggering the nucleation are investigated. The air bubble generation, transportation of nucleation sites and subsequent crystallization are discussed to improve the air injection operating conditions.

Pronounced differences exist in the biodiversity and structure of coral reef assemblages with increasing distance from shore, which may be expected given marked cross-shelf gradients in environmental conditions. Cross-shelf variation in the abundance of coral reef organisms is likely to be caused, at least in part, by differences in demography (e.g., growth and survival), though this has rarely been tested. This study quantified growth of three distinct coral taxa (Acropora nasuta, Pocillopora spp., and Stylophora pistillata) at 6 sites on Australia’s Great Barrier Reef (GBR), encompassing inshore, mid-shelf and outer-shelf reefs. Replicate colonies (up to 15 colonies per species, per reef) were stained using Alizarin red in December 2015 and retrieved 1-year later to quantify linear extension on replicate branches for each colony. Annual linear extension varied within and among coral taxa, with pronounced differences among reefs. For A. nausta. and S. pistillata, growth rates were highest at Orpheus Island, which is an inner shelf reef. However, inter-reef differences in coral growth were not explained by shelf position. Based on differences in skeletal density, which did vary according to shelf position, branching corals at the inshore sites may actually have higher rates of calcification compared to conspecifics on mid- and outer-shelf reefs. This study shows that growth of branching corals is not lower at inshore sites (and perhaps even higher) compared to sites at mid-shelf and outer reefs, despite generally higher levels of sedimentation and turbidity.

The population parameters of the ecologically important mud shrimp Upogebia major were analyzed from 2012 to 2015 in tidal flats in Seonjaedo and Jugyo on the West coast of Korea. The shrimp density averaged 265/m2 in Seonjaedo and 118/m2 in Jugyo. The sex ratio varied monthly, often male-biased, but by size, males were significantly dominant, with a carapace length (CL) over 30 mm. Although the shrimp settled almost annually, only a single size-group was observed from 2012 to 2014. The recruitment in Jugyo in 2014 was notably successful, resulting in two cohorts persisting until 2015. Many shrimp were parasitized at varying local rates: 6.0% in Seonjaedo and 37.1% in Jugyo. The growth curves revealed that the shrimp grew annually after settlement, reaching CLs of 11.90, 18.24, and 23.02 mm in Seonjaedo and 13.73, 20.86, and 25.82 mm in Jugyo. Annual mortality was 77.2% in Seonjaedo and 67.4% in Jugyo. The estimated lifespan (tmax) was 10 years in Seonjaedo and 8 years in Jugyo, whereas the 2010 cohort in Jugyo largely disappeared in 5 years. Ovigerous females appeared from November to June and benthic juveniles between June and September. The shrimp’s growth was slower relative to other East Asian populations, likely due to colder sea water temperatures and altered food conditions from a higher shrimp density.

Low temperatures in the autumn induce enhanced expression/relative accumulation of several cold-inducible transcripts/proteins with protective functions from Late-embryogenesis-abundant (LEA) superfamily including dehydrins. Several studies dealing with plants grown under controlled conditions revealed a correlation (significant quantitative relationship) between dehydrin transcript/protein relative accumulation and plant frost tolerance. However, to apply these results in breeding, field experiments are necessary. The aim of the review is to provide a summary of the studies dealing with the relationships between plant acquired frost tolerance and COR/LEA transcripts/proteins relative accumulation in cereals grown in controlled and in the field conditions. The impacts of cold acclimation and vernalization processes on the ability of winter-type Triticeae to accumulate COR/LEA proteins are discussed. The factors determining dehydrin relative accumulation under controlled cold acclimation treatments versus field trials during winter seasons are discussed. In conclusion, it can be stated that dehydrins could be used as suitable indicators of winter surival in field-grown winter cereals but only in plant prior to the fullfilment of vernalization requirement.

Four strains of green microalgae (Scenedesmus acutus, Scenedesmus vacuolatus, Chlorella sorokiniana and Chlamydomonas reinhardtii) were compared for growth and pigment composition under photoautotrophic or heterotrophic conditions. Batch growth experiments were performed in multicultivators with online monitoring of optical density. For photoautotrophic growth, light-limited (CO2-sufficient) growth was analysed under different light intensities during exponential and deceleration growth phases. The specific growth rate, mesured during exponential phase, and the maximal biomass productivity, measured during deceleration phase, were not related to each other when different light intensities and different species were considered. This indicates species-dependent photoacclimation effects during cultivation time, which was confirmed by light-dependent changes in pigment contents and compositions when exponential and deceleration phases were compared. Except for C. reinhardtii, that does not grow on glucose, heterotrophic growth was promoted to similar extents by acetate or by glucose, however these two substrates led to different pigment compositions. Weak light increased pigment contents during heterotrophy in the four species, but was efficient in promoting growth only in S. acutus. C. sorokiniana and S. vacuolatus exhibited the best potential for heterotrophic biomass productivities both on glucose and acetate, with carotenoid (lutein) content being the highest in the former.

Background. Keyword co-occurrence analysis is a crucial tool for comprehending research trends, identifying relevant studies, and gaining insight into the connections between various concepts and topics. Objective. This study focuses on analyzing the co-occurrence of keywords using FP-growth algorithm and direct search methods. Materials and methods. The methodology involved extracting bibliometric data of Energies journal for 2023-2024 from MDPI publisher platform, keyword lemmatization and keyword co-occurrence estimation. Clustering and visualization were performed using Multidendrograms and Scimago Graphica software. Results. The results showed that the FP-growth algorithm can achieve a close match with the direct search results, which facilitates data preparation for clustering. In addition, finding the co-occurrence of three or more keywords significantly reduced the number of possible combinations, which allowed the identification of specific research topics. Conclusions. This study highlights the usefulness of the FP-growth algorithm in keyword analysis and provides insights into ways to refine search queries to abstract databases for the purpose of designing and writing literature and systematic reviews.

The inclusion of exogenous phytase in broiler P and Ca deficient diets in response to heightened concerns over excessive P excretion into the environment over the years has remarkably been documented. However, responses among these studies have been inconsistent because of the several factors affecting P utilization. For this reason, a systematic review with meta-analysis of results from forty-one studies published from 2000 to February 2024 was evaluated to: 1) quantitatively summarize the size of phytase effect on growth performance, bone strength, and mineralization in broilers fed P and Ca deficient basal diets and 2) estimate and explore the heterogeneity of the effect sizes of outcomes using sub-group and meta-regression analyses. The quality of the included studies was assessed using the Cochrane Collaboration’s SYRCLE Risk of Bias checklists for animal studies. Additionally, applying the random-effects models, Hedges’ g effect size of supplementation with phytase was calculated using the R software to determine the standardized mean difference (SMD) at a 95% confidence interval. Sub-group analysis and meta-regression further explored heterogeneity (P_SMD ≤ 0.05, I² > 50%, n ≥ 10). The meta-analysis showed that supplemental phytase increases ADFI and BWG and reduces FCR at each time point of growth (< 0.0001). Additionally, phytase supplementation consistently increased tibia ash, P and Ca, and bone strength (< 0.0001) of broilers fed P and Ca deficient diets. Results of the subgroup and meta-regression analyses showed that age and strain of broiler, dietary P source, and duration of phytase exposure significantly influence the effect size of phytase on growth and bone parameters. In conclusion, phytase can attenuate the effect of reducing dietary available phosphorus and calcium and improve ADFI, BWG, and reduced FCR, especially when added to starter diets. It further enhances bone ash, bone mineralization, and bone-breaking strength of broilers, even though the effects of bone ash and strength can be maximized in the starter phase of growth. However, the effect sizes of phytase were related to the age and strain of the broiler, dietary P source, and duration of phytase exposure rather than the dosage.

Concrete cracks have a detrimental effect on the strength properties and durability of the structures. So, repairing of concrete cracks to recover their strength parameters is more important task in civil engineering. To repair concrete cracks, MICP has been widely analysed in recent times; but, zero research is conducted to deeply investigate the repair effects of MICP on concrete cracks with rough surface by using the theoretic model. In current research, MICP with a novel mathematical model was attained by taking the precipitation of calcium carbonate (CaCO3), ureolysis, suspended biomass, geochemistry, transport of solute and biofilm growth. Furthermore, crack repair experiments were performed to check the workability of the new mathematical method. The outcomes revealed that the designed suspended biomass concentrations in cracks diminished step by step. The comparison in between the experimental results and calculated results verified the precision of migration behaviour of the suspended biomass. At the inlet, the solute concentrations and biofilm volume fractions was higher, causing in rise of yield amounts for calcium carbonate. The consumed solutes concentrations were higher for cracks of less rough surface, ultimately causing in smaller sonic time; and the values of sonic time for upper portions of cracks was smaller, showing good repair impacts. The recommended mathematical model provides a better tool that controls repair time and microbial metabolism process to report a new adjustive, bioremediation and biomineralization to concrete, which could provide a firm base for remediation of material in civil engineering field.

Interpretation of increased gain in the bovine animals is difficult to be validated due to inherent genetic variation to meet their requirement for energy and protein, and those may relate to the bull species, e.g., Thai swamp buffalo. Therein, the study aimed at investigating and predicting protein requirement systems, with providing abundant energy intake 2.20 Mcal/kg DM for maintenance and growth of Thai swamp buffaloes using the comparative prolonged feeding trial for 90 days. Sixteen bull Thai swamp buffaloes at the initial (Age: 18-24 months; BW: 233 ± 25.0 kg) were as-signed into four treatment groups, four buffaloes each, fed 5.42, 6.96, 8.94, and 10.71% DM crude protein (CP). CP intake, BW, and physiological fluid were determined. The net CP requirements for maintenance and growth of Thai swamp buffaloes were 5.41 g CP/kg W0.75 and 0.46 g CP/g average daily gain (ADG), respectively. Our results indicated that CP requirement increases, when BW increases. An increased dietary CP resulted in an increased number at blood urine nitrogen (N), N absorption, total volatile fatty acid, urinary purine derivative, and the microbial N. Notably, the net CP requirement for growth of Thai swamp buffalo was higher than it reported in NRC, but the maintenance was lower.

Fibrin rich of platelets (PRF^®) of Choukroun represents a new step in the therapeutic concept of platelet gel with a simplified processing and biochemical changes little artificial. A valid method of preparation of the PRF must effectively separate the plates by erythrocytes and concentrate without damaging or lysing the plates themselves. In this study the experimental design is to standardize the production of L-PRF in horse directing it to human production. Our hypothesis is that the L-PRF is easy to produce in the horse, without modifications of the human protocol, thus allowing a better standardization of the human protocol. A new device for the preparation and the standardization of L-PRF clots and membranes is the L-PRF Wound Box^®. The optical microscopy, most cell bodies were highlighted concentrated in the proximal portion of each membrane, the last 1/4 was observed at the center; the distal part had only residual traces of cell bodies. The L-PRF will form constantly when the phases described above are strictly adhered to. The success of the art L-PRF depends entirely on the speed of blood collection and transfer in centrifuge within a minute and by a temperature of centrifugation and compression is higher than 21°C (between 21 and 30°C). Our experiments on the horse will no doubt be able to improve our understanding on wound healing, in particularly in chronic skin lesions therapy.

The sustainable development of resource-based cities has long been a focal point for both governmental policies and academic research. A wealth of academic literature identifies the 'resource curse' as a critical barrier to sustainable development in these urban areas. Despite extensive studies, a consensus on effective strategies to circumvent or resolve this challenge remains elusive. Recent empirical data reveals a noticeable pattern of economic growth differentiation in China's resource-based cities. Why do some resource-based cities experience economic decline while others achieve sustainable economic growth? Therefore, this paper analyzes the reasons for the divergence of economic growth in resource cities from the perspective of heterogeneity. The results of the study show that the phenomenon of the resource curse is conditional, and the heterogeneity of demo-graphic changes, geographic location and institutional environment has a significant impact on the economic growth of resource cities. These factors are investigated to elucidate the observed diversification and heterogeneity in the economic growth of resource-based cities. Building upon this analysis, the paper proposes a framework for differentiated sustainable development pathways tailored to the unique contexts of these cities. This approach not only contributes to the current understanding of the 'resource curse' but also offers novel insights for future research aiming to adopt a more nuanced and context-specific perspective in examining sustainable urban development.

In China, the demand for rabbit meat is also increasing, and the production for rabbit meat is important. However, the underlying mechanisms of regulate skeletal muscle growth and development in rabbits remain unclear. The purpose of this study was to identify candidate genes related to skeletal muscle growth in rabbits and explore their potential regulatory mechanisms. RNA-seq technique was used to compare the differences of skeletal muscle transcriptome in Fujian white rabbits at different developmental stages (day 20 and 26 of embryos and birth 1, 30 and 60-day-olds) with a total of 25 individual selected. A total of 9737 DEGs that were acquired from the five groups were annotated into three ontologies of the GO database: biological process (BP), cell component (CC), and molecular function (MF). For KEGG analysis, there are 8249 genes were enriched in 1148 pathways, of which 67 pathways were significantly enriched, mainly in Hypertrophic cardiomyopathy (HCM), the PPAR signaling pathway, MAPK signaling pathway. Among all comparison groups, SE was the most abundant AS event. Real-time PCR verified that the expression patterns of differential genes were consistent with the transcriptome sequencing results. These results will provide a molecular regulation mechanism of muscle growth and development in Fujian white rabbits, and should serve an important theoretical basis for improving meat performance and growth rate of Chinese local meat rabbit breeds.

The present study documented the presence of seagrass Halodule uninervis for the first time along with previously documented Halophila ovalis at Haripur creek. The population structure of both these seagrass species is assessed. The physico-chemical parameters were similar for both seagrass species except for the sediment grain size fractions. The sand content of H. ovalis patches was 1.2-fold higher than H. uninervis beds, whereas the silt content of H. uninervis beds was 2-fold higher than H. ovalis patches. The pH levels were lower than the standard oceanic pH of 8.2. Macroalgae like Ceramium sp. and Gracilaria verrucosa were growing on the leaves of H. uninervis due to high nitrate and phosphate levels of the creek waters. Leaf reddening was only observed in the leaves of H. ovalis. Under similar environmental conditions, H. ovalis (5004 ± 114.51 ind. m-²) had a 2-fold lower shoot density than that of the H. uninervis (11598 ± 187.52 ind. m-²). Both above- and below-ground biomass of H. ovalis (96.34 ± 10.18 and 197.5 ± 18.30 g DW m^-2) was 2-fold lower than that of H. uninervis (198 ±7.45 and 456 ± 9.59 g DW m^-2). H. uninervis leaves were 9-fold longer than that of H. ovalis, whereas H. ovalis leaves were 5-fold wider than H. uninervis. The leaf plastochrone interval is 2.3 days for H. ovalis and 9.6 days for H. uninervis. Consequently, the leaf growth rate of H. ovalis is 2-fold lower than that of H. uninervis. H. ovalis had 2.6-fold longer internodes than H. uninervis. The root length of H. uninervis was longer than H. ovalis. Consequently, the shorter root length of H. ovalis led to higher branching frequency than H. uninervis. The total C and N content were higher in the leaves of H. ovalis than H. uninervis.

Background: Lung cancer (LC) is a major leading cause of death worldwide. Immunomodulators that target several immune mechanisms have proven to reduce tumor burden in experimental models through induction of the immune microenvironment. We hypothesized that other biological mechanisms may also favor tumor burden reduction in lung cancer-bearing mice treated with immunomodulators. Methods: Tumor weight, area, and immune cells (T, B, macrophages, and TNF-alpha levels, immunohistochemistry) and tumor growth, oxidative stress, apoptosis, autophagy, and sirtuin-1 markers were analyzed (immunoblotting) in subcutaneous tumor of BALB/c mice injected with LP07 adenocarcinoma cells treated with monoclonal antibodies (CD-137, CTLA-4, PD-1, and CD-19, N=9/group) and non-treated control animals. Results: Compared to non-treated cancer mice, in tumors of monoclonal-treated animals, tumor area and weight and ki-67 significantly reduced, while T cell counts, oxidative stress, apoptosis, autophagy, and sirtuin-1 marker increased. Conclusion: Immunomodulators elicited a reduction in tumor burden (reduced tumor size and weight) through decreased tumor proliferation and increased oxidative stress, apoptosis, autophagy, and sirtuin-1 levels, which may have interfered with the immune profile of the tumor microenvironment. Future research should be devoted to the elucidation of the specific contribution of each biological mechanism to the reduced tumor burden.

The study investigated the effect of a multi-strain probiotic compound containing Bacillus coagulans, Enterococcus faecalis, Clostridium butyricum and Bacillus mesentericus as in-feed zinc oxide (ZnO) alterna-tive on growth performance, diarrhea incidence, antioxidant status, lipid profile, stress and immunity in weaned piglets. A total of 72 piglets were randomly divided into 3 groups with four replicates of six piglets each. The details of the groups were as follows; (i) weaned control group (WC) received basal diet, (ii) weaned probiotic group (WB) received basal diet and probiotics and (iii) positive control (PC) group received basal diet with 2500 mg/kg ZnO. The experiment lasted for 28 days. Probiotic supple-mentation improved growth performance and reduced diarrhea rate. Probiotics supplementation im-proved lipid profile; significantly lower levels of total cholesterol and low-density lipoprotein cholesterol and higher level of high-density lipoprotein cholesterol in WB group as compared to those of the control group (WC) were recorded. Probiotic supplementation stimulated antioxidant defense system by in-creasing total antioxidant capacity and decreasing lipid peroxidation. Probiotic supplementation down-regulated the stress biomarkers like serum cortisol and serum heat shock proteins. WB group showed higher serum levels of IgG and IgM throughout the study period and higher IgA at day 28 as compared to WC. These data suggest that supplementation of the probiotic minimizes the weaning stress, thereby improves the growth performance, lipid profile, antioxidant status and systemic as well as mucosal immunity. Therefore, the multi-strain probiotic compound may be used to replace ZnO in weaned piglets.

The blood-brain barrier (BBB) is a selectively permeable boundary that separates the circulating blood from the extracellular fluid of the brain and is an essential component for brain homeostasis. In glioblastoma (GBM), the BBB of peritumoral vessels is often disrupted. Pericytes, being important to maintain the BBB integrity, can be functionally modified by GBM cells by inducing proliferation and cell motility via the TGF-β-mediated induction of central epithelial to mesenchymal transition (EMT) factors., We demonstrate that pericytes strengthen the integrity of the BBB in primary endothelial cell/pericyte co-cultures as in vitro BBB model, using TEER measurement of the barrier integrity. In contrast, this effect was abrogated by TGF-β or conditioned medium from TGF-β secreting GBM cells, finally leading to the disruption of a so far intact and tight BBB. TGF-β dramatically changed the metabolic behavior of pericytes, such as shutting down the TCA cycle, driving energy generation from oxidative phosphorylation towards glycolysis, and by shifting the cells towards the activation of pathways that are necessary to produce molecules used for proliferation and cell division. Furthermore, combined metabolomics and RNASeq analyses indicated that the observed functional changes of TGF-β-treated pericytes are closely connected with their behavior.

TmMgB5O10 spontaneous crystals were synthesized via flux-growth technique from a K2Mo3O10-based solvent. The crystal structure of the compound was solved and refined within the space group P21/n. The first principles calculations of the electronic structure reveal that TmMg-pentaborate is an insulator with an indirect energy band gap of approximately 6.37 eV. Differential scanning calorimetry measurements and powder X-ray diffraction studies of heat-treated solids show that TmMgB5O10 is an incongruent melting compound. A characteristic band of Tm3+ cation corresponding to the 3H6 → 1D2 transition is observed in the photoluminescence excitation spectra of TmMg-borate. The as-obtained crystals exhibit intense blue emission with the emission peaks centered at 455, 479, 667 and 753 nm. The most intensive band corresponds to the 1D2 3F4 transition.

Plant growth regulators (PGRs) and deficit irrigation (DI) play a crucial strategic role in sustaining agricultural yield and reducing water stress in drought prone areas. The study was carried out in 2021-2022 to assess the impact of various PGRs (SA & NAA) & irrigation levels (60% Epan, 80 % Epan, and 100 % Epan) with DI & partial root drying (PRD) irrigation strategies. PGR application boosted fruit yields and demonstrated its significance in reducing water stress by lowering canopy temperature, maintaining higher relative water content in the leaves, modifying stomatal opening, and increasing photosynthetic rate. PGRs were used to assess the increased accumulation of total phenols, flavonoids, and improved antioxidant activity. Results indicated that exogenous use of PGRs like SA+NAA in conjunction with 80% irrigation levels increased fruit yield (31.97 and 40.38%) and water productivity (64.92 and 75.39%) for DI and PRD treatments, respectively. Thus standardizing irrigation and crop management practices including PGR application can be recommended to boost pomegranate productivity particularly in water-scarce environments.

Control over the composition of III-V ternary nanowires grown by the vapor-liquid-solid (VLS) method is essential for bandgap engineering in such nanomaterials and fabrication of functional nanowire heterostructures for a variety of applications. From the fundamental viewpoint, III-V ternary nanowires based on group V intermix (InSbxAs1-x, InPxAs1-x, GaPxAs1-x and many others) present the most difficult case, because the concentrations of highly volatile group V atoms in a catalyst droplet are beyond the detection limit of any characterization technique and therefore principally unknown. Here, we present a model for the vapor-solid distribution of such nanowires, which fully circumvents the uncertainties that remained in the theory so far, and link the nanowire composition to the well-controlled parameters of vapor. The unknown concentrations of group V atoms in the droplet do not enter the distribution, despite of the fact that a growing solid is surrounded by the liquid phase. The model fits quite well the available data on the vapor-solid distributions of VLS InSbxAs1-x, InPxAs1-x and GaPxAs1-x nanowires grown with different catalyst. Even more importantly, it provides a basis for the compositional control of III-V ternary nanowires based on group V intermix, and can be extended over other material systems where two highly volatile elements enter a ternary solid alloy through a liquid phase.

The study evaluated the impact of supplementing ZH in combination with D3 on growth performance, energy efficiency, carcass traits, and meat quality of feedlot lambs. Thirty-two Dorper x Katahdin cross lambs (37.3±5.72 kg) were utilized in a 29 d experiment in a complete randomized block design with a 2x2 factorial structure consisting of two levels of ZH for 26 d (0 and 0.20 mg /kg PV-1) and two levels of D3 for 7 d (0 and 1.5x106 IU/d-1). ZH improved (P≤0.05) average daily gain (ADG) and feed efficiency by 9.9% and 17.8%, respectively, as well as hot carcass weight (HCW) and dressing carcass by 4.3% and 2.6%, respectively. (p≤0.03). However, ZH increased (p<0.01) muscle pH and Warner-Bratzler shear force (WBSF) (2.5 and 23.0%, respectively). D3 supplementation negatively affected (P≤0.02) dry matter intake (DMI) (last 7 d) and ADG by 15.7% and 18.1%. On the other hand, D3 improved the pH of the longissimus muscle (LM) by 1.7% (p=0.03) without affecting WBSF. When D3 was supplemented in combination with ZH, it was observed that meat quality was improved by reducing muscle pH compared to lambs treated only with ZH. However, D3 did not improve meat tenderness produced by ZH supplementation.

The dynamic process of plants’ growth is primarily influenced by interconnected biochemical and physiological activities that operate throughout different growing phases. These processes respond to environmental conditions, affecting plant development and productivity. The present studies were carried out to investigate greenhouse conditions' impact on winter-grown cherry tomatoes (Cheramy F1) in terms of growth, yield, and nutritional composition.Data was collected on various dates during the period extending from December 2022 to March 2023. The greenhouse temperature varies from 22°C to 25.47°C (avg. 21.20°C) during plant growth and from 20°C to 23.85°C during fruit-bearing. Average CO2 concentration was 349.19 ppm; average light was 240.93 w/m2. Measured growth parameters include plant height, leaf growth, stem diameter, leaf spacing, leaf count, leaf area, and inflorescence count per plant. The key results of this study revealed the progressive growth report (PGR), which predicted the daily potential growth rate of plants: plant height, 2.86 to 3.81 cm/day; growth rate of mature old leaf: 0.003988 m2/day; middle younger leaf: 0.008733 m2/day; top Nascent leaf: 0.010722 m2/day; three to five leaves per week; and one inflorescence per week. In our accidental observation, we observed that plants with different orientations and growing spots within the greenhouse responded differently in view of their growth parameters. A exceedingly significant interaction among the inflorescence was found in view of length of inflorescence, number of fruits inflorescence, total fruit mass inflorescence, average fruit mass inflorescence, fruit dry matter inflorescence, fruit height inflorescence, and fruit diameter inflorescence. A non-significant interaction was found between the fruit keeping quality (Shelf days), fruit size, and inflorescence number. Inflorescence with a high, lower leaf area appeared to have high productivity. The nutritional composition of fruit was also different among the inflorescences.. The study's goal is to investigate and gather data to test the hypothesis that changes in greenhouse conditions and the adoption of green management practices will directly influence plant growth attributes, productivity, and fruit quality. The findings of this research could be valuable for optimizing greenhouse operations to enhance crop yields and quality while also promoting environmental sustainability.

The corrosion behavior of Fe-containing directionally solidified (DS) and centrifugally cast (CC) Al-Si-Cu-Zn alloys with either Co or Ni additions has been investigated. Electrochemical and immersion corrosion methods were used to investigate the corrosion behavior in 0.6 M NaCl after short (1-hour) and long (30-day) exposure periods. The employed solidification methods allowed the production of samples with a wide range of secondary dendrite arm spacing (SDAS) while preserving Si and Fe-containing phases. The 0.5 wt.% Ni and Co additions led to the growth of the AlFeSi(Ni) and AlFeSi(Co) phases, but no binary AlNi nor AlCo intermetallic par-ticles have been generated. Potentiodynamic polarization studies at early exposure revealed an increase in the corrosion potential as the Ni was added for either fast or slow solidified samples. The electrochemical impedance spectroscopy at early exposure demonstrated that the Ni-modified alloy, on the other hand, was associated to smaller charge transfer resistances, in-dicating a reduction in corrosion resistance after short elapsed time into the electrolyte. Howev-er, 30-day immersion tests revealed much lower corrosion rate of the Ni-modified alloy than the other alloys, while the corrosion rates of the Co-modified and non-modified alloys were simi-lar. In the Ni-containing alloy, decreased corrosion rate under long-term corrosion process was attributed to the formation of a thick and dense alumina layer, effectively protecting the surface under such conditions. This work contributes to a better knowledge of the corrosion behavior of Ni- and Co-corrected Al industrial scrap compositions.

The longleaf pine (Pinus palustris P. Mill.) forest type occupied a much greater area in the United States prior to the arrival of Europeans, estimated to be around 37.2 million hectares. This area has been greatly reduced, and these ecosystems now occupy only about 1.2 to 1.6 million hectares. However, there has been great interest in the restoration of this forest type, and existing stands are actively managed. Thus, managers need tools to estimate site quality and ultimately productivity. Here, 16 existing equations to estimate site index and dominant height in naturally regenerated longleaf pine stands are examined for their behavior across a range of site quality and age conditions. Others can use this information as a reference to determine which equation may be best for their situation.

Definitive surgical resection is the preferred treatment for early-stage non-small-cell lung cancer (NSCLC). Research into genetic alterations, including epidermal growth factor receptor (EGFR) mutation, in early stage NSCLC remains insufficient. Here, we investigated the prevalence of genetic alterations in early-stage NSCLC and the association between EGFR mutation and recurrence after complete resection. Between January 2019 and December 2021, 659 patients with NSCLC who underwent curative surgical resection at a single regional cancer center were recruited. We compared the clinical and pathological data between the recurrence and non-recurrence groups. Multivariate logistic regression was used to predict the risk factors for recurrence. Among the 659 enrolled cases, the most common histology was adenocarcinoma (74.5%), followed by squamous cell carcinoma (21.7%). The prevalence of EGFR mutation was 43% (194/451). Among them, L858R point mutation and exon 19 deletion was 52.3% and 42%, respectively. ALK rearrangement was found at 5.7% (26/453), and ROS1 fusion was found at 1.6% (7/441). The recurrence rate of the entire population was 19.7%. In multivariate analysis, the presence of EGFR mutation, stage II or III (vs. stage I), and pathologic subtype (presence of solid type) were associated with recurrence. Among the recurred group, 86.5% of the patients with EGFR mutation experienced distant recurrence compared to only 66.7% of wild-type (p = 0.016), with no significant difference in median disease-free survival (p = 0.983). In conclusion, the prevalence of EGFR mutation, ALK rearrangement, and ROS1 fusion was 43.0%, 5.7%, and 1.6%, respectively in patients with early-stage NSCLC who underwent curative resection. Along with stage II/III and solid pathologic subtype, EGFR mutation was an independent risk factor for recurrence. In the recurrence group, the rate of distant metastasis was higher in patients with EGFR mutation than in those with wild-type.

Modern technologies can satisfy human needs only with the use of large quantities of fertilizers and pesticides that are harmful to the environment. For this reason, it is possible to develop new technologies for sustainable agriculture. The process could be made by using endophytic microorganisms with a (possible) positive effect on plant vitality. Bacterial endophytes have been reported as plant growth promoters in several kinds of plants under normal and stress conditions. In this study, isolates of bacterial endophytes from the roots and leaves of Miscanthus giganteus plants were tested for the presence of plant growth-promoting properties and their ability to inhibit pathogens of fungal origin. Pantoea ananatis and Pseudomonas libanensis were the predominant bacteria in leaves whereas other pseudomonads prevailed in roots. Selected bacterial isolates were able to solubilize inorganic phosphorus, to fix nitrogen, and to produce IAA, ACC deaminase and siderophore. Leaf bacterial isolate Pantoea ananatis 50 OL 2 had high production of siderophores and weak capabilities for phytohormone production. The root bacterial isolate of Pseudomonas libanensis 5 OK 7A showed the best results of phytohormone production. Four fungal cultures - Fusarium sporotrichioides DBM 3229, Sclerotinia sclerotiorum SS-01, Botrytis cinerea and Sphaerodes fimicola were used to test the antifungal activity and these cultures were selected for their representatives of known pathogenic fungi families, especially for crops. All selected roots endophyte isolates significantly inhibited the pathogenic growth of Fusarium sporotrichoides with a mean inhibition diamater (zone ≥ 5 mm). These results demonstrated that the root endophytic Pseudomonas sp. could be used as biofertilizer for crops.

A model to rationalize the effects of test temperature and microstructural variables on the creep crack growth (CCG) and creep-fatigue crack growth (CFCG) rates in ferritic steels is described. The model predicts that as the average spacing between grain boundary particles that initiate creep cavities decrease, the CCG and CFCG rates increase. Further, CCG data at several temperatures collapse into a single trend when a temperature compensated CCG rate derived from the model is used. CCG and CFCG behavior measured at different temperatures is used to assess the effects of variables such as differences between basemetal (BM), weldmetal (WM) and the heat-affected zone (HAZ) regions. The model is demonstrated for Grade 22 and Grade 91 steels using data from literature. It is shown that differences between the CCG behavior of Grade 22 steel in new and ex-service conditions are negligible in the BM and WM regions but not in the HAZ region. The CCG behavior of Grade 91 steels can be separated into creep-ductile and creep-brittle regions. The creep-brittle tendency is linked to the presence of excess trace element concentrations in the material chemistry. Significant differences found in the CCG rates between BM and the WM and HAZ regions of Grade 91 steel were explained.

The MET proto-oncogene encodes a pivotal tyrosine kinase receptor, binding the hepatocyte growth factor (HGF, also known as scatter factor, SF) and governing essential biological processes such as organogenesis, tissue repair, and angiogenesis. The pleiotropic physiological functions of MET explain its diverse role in cancer progression in a broad range of tumors; genetic/epigenetic alterations of MET drive tumor cell dissemination, metastasis, and acquired resistance to conventional and targeted therapies. Therefore, targeting MET emerged as a promising strategy, and many efforts were devoted to identifying the optimal way of hampering MET signaling. Despite encouraging results, however, the complexity of MET's functions in oncogenesis yields intriguing observations, fostering a humbler stance on our comprehension. This review explores recent discoveries concerning MET alterations in cancer, elucidating their biological repercussions, discussing therapeutic avenues, and outlining future directions. By contextualizing the research question and articulating the study's purpose, this work navigates MET biology's intricacies in cancer, offering a comprehensive perspective.

This paper examines the relationship between GDP per capita and waste generated in European Union countries, highlighting the importance of this relationship for understanding the environmental consequences of economic growth and formulating waste management policies. The analysis findings demonstrate that, in most cases, higher GDP is associated with higher amounts of waste, although there are exceptions to this trend. A smaller number of countries are also found where no such relationship exists. These are primarily high-income countries in the lower part of the EKC or countries in transition. Cluster analysis reveals regional differences in the relationship between GDP and waste that the economic factors and the level of development in the regions concerned can explain. Bulgaria is among the European countries with the lowest economic growth associated with a large amount of waste. The analysis, therefore, focuses on Bulgaria. The impact of GDP per capita, Human Development Index and Population Density on municipal waste in Bulgaria is studied. The aim is to identify significant relationships and dependencies between these factors and the amount of waste. Based on the results of multiple regression analysis and the findings from municipal waste management data in Bulgaria, specific actions are proposed for municipal waste management in the country. Recommendations are made for developing sustainable waste management policies and sharing best practices between regions.

Typhulaceae Jülich is one of the cold-adapted fungal families in basidiomycetes. Typhula (Pers.) Fr. and Pistillaria Fr. representing the family are distinguished from the morphology of a stipe to a hymenium (Fries 1821). The hymenium of Typhula is distinguishable from the stipe, but indistinguishable and consecutive in the genus Pistillaria. This taxonomic criterion is ambiguous, and consequently the opinion of Karsten (1882) has been widely accepted; I the genus Typhula, basidiomata develop from sclerotia, while basidiomata develop directly from substrata in the genus Pistillaris. But, Corner (1970) observed basidiomata of Pistillaria petasitis S. Imai in Hokkaido, Japan, from sclerotia. We collected irregular sclerotia of Typhula hyperborea H. Ekstr. from Upernavik, West Greenland. This specimen had a stipe-like structure on a Poaceae plant, and sclerotia developed on its tip. In August, P. petasitis in Hokkaido, Japan, formed basidiomata directly from substrates on the ground. Similar phenomena were found from other Typhula spp. in Japan. All of these fungi had the potential to produce sclerotia in culture, and as well as the capacity of mycelial growth at ambient air temperatures in each locality when samples were collected. These findings suggest that Typhula spp. developed basidiomata not only from the sclerotia dispersed by the basidiospores but also mycelia generated by the spore germination formed basidiomata multiple times depending on their growth environments.

Alfalfa primarily produces seeds through cross-pollination among different individuals. Self-pollination results in severe inbreeding depression, such as weak seedlings and termination of growth. Identifying the genetic loci associated with vigorous plant growth could enable the elimination of deleterious alleles and eventually develop inbred alfalfa lines for hybrid production. In this study, 215 alfalfa accessions were self-pollinated for three generations. Within accessions, pairs of weak and strong plants were sampled and bulked for exome sequencing. We extracted individual DNA from 534 plants that included parental clones, strong and weak pairs of plants, and plants selected based on the number of seeds produced. Among them, we formed 42 pools, including 16 with strong plants and 17 with weak plants, 3 top-seeded plants, 3 low-seeded plants, and 3 no-seeded plants. Along with 79 individuals, these pools were sequenced in the target regions covering 112,626 contigs across the entire alfalfa genome. From the 121 samples, (42+79) genotyped, 13.2 million SNPs including indels were generated. After filtering for MAF (>5%), depth (<20X), and no missing genotype and removing indels, a total of 588,136 SNPs were obtained which were used for final analysis. A genome-wide association study was performed and identified 11 genetic loci associated with alfalfa plant growth vigor. The GO analysis generated 12 significantly enriched GO terms. The associated SNPs were on, and near genes involved in stress response, defense responses against pathogens, and plant reproduction. These identified SNPs benefit the development of alfalfa inbred lines by purging the deleterious alleles and biomass improvement through marker-assisted selection.

Psychomotor developmental delay in infants includes failure to acquire abilities such as sitting, walking, grasping objects and communication at the ages when most infants have acquired these abilities. Known risk factors include a large number of aspects of family environment, socioeconomic position, problems in pregnancy and birth, and maternal health. It is clinically useful to be able to screen for developmental delay so that healthcare interventions can be considered. The present research used machine learning (random forest) to create an algorithm predicting psychomotor delay in 9-month-old infants using information ascertainable at birth and in early infancy. The dataset was the UK longitudinal Millennium Cohort study. Fifty-two predictors measuring socioeconomic indicators, paternal, family and social support for the mother, beliefs about good parenting, maternal health, pregnancy and birth were included in the initial algorithm. Feature reduction showed that of the 52 variables, birthweight, family income and parents’ ages had the highest feature importance scores and could alone correctly predict developmental delay with over 99% sensitivity and 100% specificity. The relationships between delay and some of the predictors, particularly income, were nonlinear and complex. The results suggest that the risk of psychomotor developmental delay can be identified in early infancy using machine learning, and that the best predictors are factors present prior to birth. Surprisingly, the most important factors included in the present study did not include illnesses during pregnancy such as eclampsia and infections.

Studies suggest that elevated postnatal blood lead levels (BLLs) are negatively associated with child growth. This study aimed to investigate associations of childhood BLLs at age one-year and growth outcomes at age six-years (n=661) in a cohort of children in Allada, Benin. The growth outcomes studied are weight-for-age Z-score (WAZ), height-for-age Z-score (HAZ) and BMI-for-age Z-score (BMIZ), head circumference (HC), underweight, stunting, and wasting. Multivariable regression models examined associations between blood lead levels, growth outcomes with adjustment for potential confounders. The geometric mean BLLs was 59.3 μg/L and 82% of children had BLLs &gt;35 μg/L at one year. After adjusting for confounding factors, no association was found between BLLs quartiles and HAZ, WAZ, BMIZ, and height and weight growth velocities. However, boys in the highest quartile had 1.02 cm lower HC (95% CI: [-1.81, -0. 24]) as compared to those in the lowest quartile with a dose-response trend across quartiles (Ptrend =0.02). Furthermore, an increased risk of being stunted was observed in children in the highest quartile of exposure compared to the first (OR: 2.43; 95% CI: [1.11 – 5.33]) with a dose-response trend (Ptrend =0.03). Blood lead was found to be associated with an increased risk of childhood stunting and lower head circumference in a resource-limited setting.

Hydrogenated small fullerenes (Cn, n<60) are of interest as potential astrochemical species, and as intermediates in hydrogen catalysed fullerene growth. However computational identification of key stable species is difficult due to the vast combinatorial space of structures. In this study we explore routes to predict stable hydrogenated small fullerenes. We show that neither local fullerene geometry nor local electronic structure analysis are able to correctly predict subsequent low energy hydrogenation sites, and indeed sequential stable addition searches also sometimes fail to identify most stable hydrogenated fullerene isomers. Of the empirical and semi-empirical methods tested, GFN2-xTB consistently gives highly accurate energy correlation (r>0.99) to full DFT-LDA calculations at a fraction of the computational cost. This allows identification of the most stable hydrogenated fullerenes up to 4H for four fullerenes, namely two isomers of C28 and C40, via “brute force” systematic testing of all symmetry inequivalent combinations. The approach shows promise for wider systematic studies of smaller hydrogenated fullerenes.

During the last two decades, energy poverty has captured a growing attention of researchers and policymakers due to its strong association with economic poverty and poor economic performance. This study uses a broad set of macro level indicators and makes the first attempt to measure energy poverty and its impact on economic growth of Pakistan over the period 1990 to 2017. In particular, our energy poverty indicator considers four main dimensions of energy poverty, namely, energy services, clean energy, energy governance and energy affordability. Our main results show that though the overall energy poverty has reduced in Pakistan during the selected sample period, the country shows an increasing dependence on polluted energy supply in order to meet its growing demand of energy. In second stage of the investigation, we test the neoclassical growth theory where we incorporate energy poverty along with human capital as source of economic growth. Our cointegration results reveal a strong relationship between energy poverty and economic growth that is also dynamically stable in short run. These strong negative linkages between energy poverty with economic growth for the sample economy complement the previous literature on the subject.

World Economy today depends on business investments that are propelled by Green technology, innovations and entrepreneurial activities. In recent years, developing economies in Asia, Africa and Latin America have embarked on easy capital access to Small Medium Enterprises (SMEs) to facilitate their economic growth. Kenya is among the Middle Level Income Countries that have gained global recognition through entrepreneurial innovations. In this study we assess the role of entrepreneurship towards poverty alleviation in Kenya. The objectives of the study were: to evaluate the role of entrepreneurship in poverty alleviation in Kenya; to identify entrepreneurship innovations and their economic contribution in Kenya and to determine the significance of entrepreneurship to Kenyan economy. Methodology of study took a parametric approach through pure desktop studies on entrepreneurship cases in Kenya. Success case studies of entrepreneurial innovations like M-pesa, M-soko and Uwezo Fund initiatives were assessed. The paper notes that activities undertaken in each of these case studies have made great contributions to poverty alleviation and economic development in both urban and rural areas of Kenya.

Fusarium graminearum causes head blight in wheat and corn, and produces chemicals harmful for humans and other animals. It is important to understand how it grows in order to prevent outbreaks. There are 3 well-known growth models for microorganisms and they seem applicable to molds: linear, Gompertz and Baranyi. This study aimed to see which could better represent F. graminearum growth. Three replicates were grown in yeast extract agar (YEA) for 20 days. The Feret’s radius was measured in ImageJ software, and then related to the models. Linear model was the most closely correlated to the actual growth. Thus, considering that it was the most representative of the reality and it is easier to use, it seems to be the best logical choice for F. graminearum growth studies.

This paper highlights the impact of financial inclusion on individuals’ borrowing and saving decisions in the United States and the United Arab Emirates. It does so, using data from the 2014 Global Financial Inclusion database, and an empirical strategy consisting of first testing the significance of the joint bivariate model over its binary conterparts using the Lagrange multiplier test, followed by a contrast between the fully-parametric and semi-parametric specifications of the saving and borrowing equations. Based on model performance measures, the semi-parametric bivariate probit specification is identified as a better framework for describing the two processes of saving and borrowing, with a correlation coefficient of 12.3%. Although no significant difference exists between UAE and US residents in borrowing behavior, we find that US residents are 31.4% more likely to save than their UAE counterparts. In addition, and in line with the Permanent Income Hypothesis, the results reveal the absence of an income based saving or borrowing gradient in the two countries. Conversely, we found the prevalence of a gender based saving (12.4%) and borrowing (13.8%) inequality in favor of the male gender. Furthermore, access to a bank account and a debit card, companies policy of direct wage and salary transfer, and government transfer programs with direct deposit options are all financial inclusion strategies that are found to significantly raise the likelihood of saving and borrowing. As such, companies with payroll practices based on cash or physical paycheck issuing, especially in the US, should revise such policy to create more financial inclusion, and thereby more saving potential which in turn would contribute to further borrowing, investment and growth of the national economy.

Layered superconductors are attractive because some of them show high critical temperatures. While their crystal structures are similar, those compounds are composed of many elements. Compounds with many elements tend to be incongruent melting compounds, thus, their single crystals cannot be grown via the melt-solidification process. Hence, these single crystals have to be grown below the decomposition temperature, and then the flux method, a very powerful tool for the growth of these single crystals with incongruent melting compounds, is used. This review shows the flux method for single-crystal growth technique by self-flux, chloride-based flux, and HPHT (high-pressure and high-temperature) flux method for many-layered superconductors: high-T_c cuprate, Fe-based and BiS₂-based compounds.

The rapid development of China’s textile industry leads to consumption and pollution of large volumes of water. Therefore, the textile industry has been the focus of water conservation and waste reduction in China’s 13th Five-Year Plan (2016–2020). The premise of sustainable development is to achieve decoupling of economic growth from water consumption and wastewater discharge. In this work, changes in blue water (water consumption), grey water (water pollutants), and water footprints of the textile industry from 2001 to 2014 were calculated. The relationship between water footprint and economic growth was then examined using the Tapio decoupling model. Furthermore, factors influencing water footprint were determined through logarithmic mean Divisia index (LMDI) method. Results show that the water footprint of China’s textile industry has strongly decoupled for five years (2002, 2006, 2008, 2011, and 2013) and weakly decoupled for four years (2002, 2007, 2009, and 2010). A decoupling trend occurred during 2001–2014, but a steady stage of decoupling has not been achieved yet. Based on the decomposition analysis, the total water footprint is mainly increased by production scale and inhibited by the technology. In addition, the effect of industrial structure adjustment is relatively weak.

To investigate the influence of supplemental lighting intensity on the production of cut gerbera during Canada’s supplemental lighting season (November to March), trials were carried out at a research greenhouse. Five supplemental LED light intensity (LI) treatments provided canopy-level photosynthetic photon flux densities (PPFD) ranging from 41 to 180 µmol·m-2·s-1. With a 12-h photoperiod, the treatments provided 1.76 to 7.72 mol·m-2·d-1 of supplemental light. Two cultivars of cut gerbera (Gerbera jamesonii H. Bolus ex Hook.f) were used to evaluate vegetative growth and flower production. Plugs of ‘Ultima’ were assessed for vegetative growth and rate of flower development. There were minor LI treatment effects on number of leaves and chlorophyll content index and flowers from plants under the highest vs. lowest LI matured 10% faster. Reproductively mature ‘Panama’ plants were assessed for flower yield and quality. ‘Panama’ flowers from the highest LI treatment had shorter stems than the three lowest LI treatments, flowers from the middle LI treatment had larger diameter than the other treatments. Flowers from the lowest LI treatment had lower fresh mass than the three highest LI treatments. There were linear relationships between LI and numbers of flowers harvested, with the highest LI treatment producing 10.3 and 7.0 more total and marketable flowers per plant than the lowest LI treatment. In general, increasing levels of supplemental light had only minor effects on vegetative growth (young plants) and size and quality of harvested flowers (mature plants) but flowers from plants grown under higher LIs were more numerous and matured faster.

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivity of red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

This paper studies the combined effect of corrosion and fatigue on the growth of cracks in aircraft and on the effect of skin corrosion and stress corrosion cracking on the load bearing capacity of rib stiffened aircraft wings. In this context it is shown that the growth of cracks from surface pitting, and also from intergranular cracking at a fastener hole, can be accurately computed using the Hartman-Schijve variant of the NASGRO crack growth equation. The examples studied support the lead crack approach, that has been independently developed by the USAF and the Australian Defence Science Technology Group, in which the growth of lead cracks is often exponential. In the case of skin corrosion it is shown that to be consistent with the US Joint Service Structural Guidelines (JSSG2006) assessment of its effect on the load bearing capacity of the wing should involve an assessment of whether at 115% DLL the remaining material exceeds the yield stress of the material.

As climate change should lead to an increase in the vulnerability and the sensitivity of forests to extreme climatic events, quantifying and predicting their response to more severe droughts remains a key task for foresters. Furthermore, recent works have suggested that tree diversity may affect forest ecosystem functioning, including their response to extreme events. In this study we aimed at testing whether the growth response of forest stands to stressful climatic events varied between mixed and monospecific stands, under various environmental conditions. We focused on beech-fir forests (Fagus sylvatica [L.] and Abies alba [L.]) and beech-oak forests (Fagus sylvatica [L.] and Quercus pubescent [L.]) in the French Alps. We used a dendrochronological dataset sampled in forest plots organized by triplets (one mixture and two monospecific stands) distributed in six sites along a latitudinal gradient. We tested (1) whether stand diversity (two-species stands vs monospecific stands) modulates the stands’ response to drought events in terms of productivity, (2) whether species identity may drive the diversity effect on resistance and recovery, and (3) whether this can be explained by interspecific interactions. We found that (1) interspecific differences in response to extreme drought events (possibly due to interspecific differences in hydraulic characteristics) can induce a mixture effect on stand growth, although it appeared (2) to be strongly depending on species identity (positive effect only found for beech-fir mixed stands), while (3) there were no significant non-additive effects of diversity on stand resistance and recovery, except for some specific cases. Overall, our study shows that promoting selected mixed stands management may buffer extrem drought effect on stand productivity.

According to some authors, the serum selenium level is strongly associated with the severity of liver diseases including liver cirrhosis. The aim of the study was to determine the relationship between the concentration of selenium and pro-inflammatory and profibrotic cytokines – interleukin-6 (IL-6) and growth differentiation factor 15 (GDF-15) in patients with alcoholic liver cirrhosis. The parameters studied were determined in serum of 99 alcoholic liver cirrhosis patients divided based on the severity of disease according to the Child-Turcotte-Pugh criteria. In patients with liver cirrhosis, the serum selenium concentration was statistically lower whereas serum IL-6 and GDF-15 concentrations were higher than those in the control group. Moreover, the concentration of selenium negatively correlated with the levels of GDF-15 and IL-6. The above results may indicate a role of selenium deficiency in the pathogenesis and progression of alcoholic liver disease.

Astronauts at are risk of losing 1.0 – 1.5% of their bone mass for every month they spend in space despite their adherence to high impact exercise training programs designed to preserve the musculoskeletal system. This article reviews the basics of bone formation and resorption and details how exposure to microgravity or simulated microgravity affects the structure and function of osteoblasts, osteocytes, osteoclasts, and their mesenchymal and hematologic stem cell precursors. It details the critical roles that insulin-like growth facor-1 and its receptor IGFR1 play in maintaining bone homeostasis and how exposure of bone cells to microgravity affects the function of these growth factors. Lastly, it discusses the potential of tumor necrosis factor-related apoptosis-inducing ligand, syncytin-A, and sclerostin inhibitors and recombinant IGF-1 as a bone-saving treatment for astronauts in space and during their colonization of the Moon.

We assessed the growth performance, physiological traits, and gene expressions in steers fed with dietary rumen-protected L-tryptophan (RPT) under cold environment. Eight Korean native steers were assigned to two dietary groups, no RPT (Control) and RPT (0.1% RPT supplementation on a dry matter basis), 6 wks. Maximum and minimum temperatures throughout the experiment were 6.7°C and -7.0°C, respectively. Supplementation of 0.1% RPT to a total mixed ration did not increase body weight but had positive effects of elevating average daily gain (ADG) and reducing the feed conversion ratio (FCR) at day 27 and 48. Metabolic parameter showed higher glucose level (at day 27) in the 0.1% RPT group compared to the control group. Real-time PCR analysis showed no significant differences in the expression of muscle (MYF6, MyoD, and Desmin) metabolism genes between the two groups, whereas the expression of fat (PPARγ, C/EBPα, and FABP4) metabolism genes was lower in the 0.1% RPT group than in the control group. Thus, we demonstrate that long-term (6 wks) dietary supplementation of 0.1% RPT was beneficial owing to enhanced growth performance by increasing ADG and glucose level, decreasing FCR, and maintaining homeostasis in immune responses in beef steers during cold environment.

This study aims to explore predictors of the foreign investment from China coming into Rwanda. One of the components of Rwanda’s economy is the investment it receives from other countries. In order to achieve these objectives, the study gathers econometric secondary data from 2007 to 2020 quarterly which were availably provided by National Statistics of China, World Bank, UNCTAD, National Institute of Statistics of Rwanda, and the National bank of Rwanda. From literature and based on the availability of data, market size, trade openness, infrastructure, and human capital were measured as predictors of Chinese FDI. The data is analyzed using linear regression in Stata. The finding of the study showed that variables have a positive effect on FDI from China, and it found that though FDI had an effect on overall economy of Rwanda, the effect was not statistically significant. The study suggested that Rwanda’s policy on foreign investment should aim to attract and encourage Chinese investment to increase the economy of Rwanda. To encourage more FDI, the Rwandan government can offer Chinese investors greater ownership, locational, and internalization benefits. It also should continue to strengthen economic policy transparency since it lowers transaction costs and hence improves incentives for foreign investment.

Multiaxial fatigue experiments under asynchronous loadings with four different loading frequency ratios were carried out on 30CrMnSiA steel. The experimental results show that the fatigue life decreases when the axial or torsion frequency increases from 1 to 2, while there is no significant change when the axial or torsion frequency increases from 2 to 4. The surface crack paths are observed and show that cracks initiate on the maximum shear stress amplitude planes, propagate approximately tens of microns, and then turn to propagate along the maximum normal stress planes. The number of secondary cracks increases when the axial or torsion frequency increases. Subsequently, the Bannantine-Socie and Wang-Brown cycle counting methods along with various multiaxial fatigue criteria and Palmgren-Miner’s cumulative damage rule were used for fatigue life prediction. The experimental results are consistent with the fatigue life predicted by the Bannantine-Socie method with the section critical plane criterion for 30CrMnSiA steel under asynchronous loading paths.

Peyronie's disease (PD) is a benign condition caused by plaque formation on the tunica albuginea of the penis. It is associated with penile pain, curvature, and shortening, and contributes to erectile dysfunction, which worsens patient quality of life. In recent years, understanding of the detailed mechanisms and risk factors involved in development of PD has been increasing. In this review, the pathological mechanisms and several closely related signaling pathways, including TGF-β, WNT/β-catenin, Hedgehog, YAP/TAZ, MAPK, ROCK, and PI3K/AKT, are described. Findings regarding cross-talk among these pathways are then discussed to elucidate the complicated cascade behind tunica albuginea fibrosis. Finally, various risk factors including genetic involved in the development of PD are presented and their association with the disease summarized. The purpose of this review is to provide better understanding regarding involvement of risk factors in the molecular mechanisms associated with PD pathogenesis, as well as provide insight into disease prevention and novel therapeutic interventions.

This study was conducted to propose the Green Economic Development Plan for Manufacturing S.M.E.s based on financial performance and operations thru Green Initiatives. The descriptive survey method used to gather data to determine the green initiative's implementation of small and medium (S.M.E.s) manufacturing enterprises. Green initiatives use as a basis for crafting a green economic development plan. Document analysis was also employed to obtain data from any available printed materials and records provided by the respondents. Such methods of gathering information used to validate data gathered from local and foreign-related literature. The observation also employed to survey the assets owned and validate any green initiatives practiced, including their implementation. The study's findings show that among the green initiatives implemented by SMMEs, which resulted in the reduction of total costs and expenses, were the proper disposal and segregation of waste materials, water management by recycling wastewater and using water-efficient equipment, natural resources, and raw materials management. SMMEs should encourage active participation and support of suppliers and customers in achieving G.E.D.'s objectives by developing incentive schemes.Furthermore, SMMEs should continue to benchmark with G.E.D. Practitioners are operating locally and abroad to adopt best greening strategies and regularly network with concerned government agencies for continuous updating on G.E.D. Initiatives that may benefit the firm. Further research may be conducted on green initiatives implemented by small and medium enterprises in other industry sectors.

Bacteria belonging to the genus Micromonospora are recognized as microorganisms with potential to be used in biotechnology processes, including their beneficial influence on plant growth and biocontrol of phytopathogens. In this study, nineteen Micromonospora isolates originating from root nodules of white clover plants were taxonomically assigned based on the phylogenetic analysis of the 16S rRNA gene and four housekeeping genes. The antifungal properties of the bacteria against phytopathogenic Botrytis cinerea, Fusarium oxysporum, Fusarium equiseti, Sclerotinia sclerotiorum, and Verticillium albo-atrum were tested with the agar plug test and the dual culture test. The ability to produce various metallophores was determined with the agar plug diffusion test on modified CAS agar medium. ISP2 broth amended with 0.2% L-tryptophan was used to indicate the bacterial ability to produce auxins. Strains belonging to M. tulbaghiae, M. inaquosa, and M. violae showed in vitro potential as antimicrobial agents against the tested fungi. M. inaquosa strain 152, M. violae strain 126, M. violae strain 66 and M. violae strain 45 were recognized as the most efficiency metallophores producers. M. alfalfae strain 55 and M. lupini strain 5052 were identified as the most promising auxin compound producers and, therefore, as potential plant growth promoting bacteria.

Streptomyces sp. HNS054, which was previously isolated from a marine sponge, demonstrates vigorous growth and facile genetic modification. This study explores its potential as a marine ac-tinomycete chassis through complete genome sequencing and genetic engineering. With a 7.5 Mb genome containing 6678 predicted genes, 21 secondary metabolic biosynthetic gene clusters (BGCs), and 8 common site-specific recombination (SSR) system attB loci, HNS054 shows a close phylogenetic relation to S. griseoincarnatus strain RB7AG. Utilizing multiplexed site-specific ge-nome engineering (MSGE) and the CRISPR/Cas9 method, engineered strains derived from HNS054 (with three copies of the target BGCs) produce higher amounts of aborycin and ac-tinorhodin than S. coelicolor M1346 (containing three copies of the same BGCs). HNS054 stands out for its remarkable characteristics, including salt tolerance, rapid growth, and compatibility with synthetic biology tools, and positions it as a promising candidate for developing marine ac-tinomycete hosts to enhance secondary metabolite production.

Understanding the implications of organic acids in animal diets on physiological markers is critical for developing viable strategies for improving overall health and performance. The aim of this study was to evaluate the influence of dietary organic acids inclusion on rabbits’ growth performance and physiological status by determining blood and intestine development indicators. Forty weaned rabbits aged 28-d randomly assigned into 2 groups and fed with standard compound diet (SCD) and SCD + 0.2% organic acids mixture (butyric, propionic, sorbic; OAM). Growth performance was evaluated between 28-77 d of age, physiological status at 77 d of age. OAM’s rabbits final body weight, ADG, DFI, growth rate indicators were higher, but FCR lower compared to SCD (P &lt; 0.05). OAM increased total protein and albumin levels in blood plasma; reduced pH, increased DM, achieved greater viscosity in caecum’s content (P &lt; 0.05). Compared to SCD better gut development observed in OAM, as total intestinal weight was significantly higher (P &lt; 0.05). Acetic and propionic acids concentrations were increased, but butyric reduced in OAM caecum’s content (P &lt; 0.05). OAM had significant effect on the most caecum histomorphometric parameters: increased villus height, crypt depth and their ratio (P &lt; 0.05). According to our results OAM can improve rabbits’ growth performance, nutrient intake, and feed digestibility by significantly improving physiological status.

Microbial-based biostimulants containing arbuscular mycorrhizal fungi (AMF), Trichoderma fungi and plant growth-promoting rhizobacteria (PGPR) have been applied in an open-field tomato cultivation. A two-years field experiment (2020-2021) was performed in Southern Italy on “Heinz 1534” processing tomato hybrid, using three commercial formulations characterized by different microbial consortia (MIC: Glomus spp., Rhizophagus spp., Bacillus spp., Streptomyces spp., Pichia spp., Trichoderma spp.; EKO: Glomus spp., Bacillus spp., Streptomyces spp., Pseudomonas spp., Arthrobotrys spp., Monacrosporium spp., Paecilomyces spp., Myrothecium spp., Trichoderma spp.; FID: Glomus spp., Bacillus spp., Trichoderma spp.) and comparing them to untreated control (CTRL). The effect of growing season and microorganism-based treatments on yield, technological traits and functional quality of tomato fruits was assessed. The year of cultivation (Y) affected yield (with lower fruit weight, higher marketable to total yield ratio and higher % of total defected fruits in 2020), and technological components (higher dry matter, total acidity, total soluble solids in 2020). During the first year of the trial, all evaluated treatments (MIC, EKO and FID) enhanced soluble solids content by 10%, on average, compared to CTRL. Sucrose and lycopene contents were influenced both by the microbial-based treatments and the growing season (greater values found in 2021 with respect to the first year). Y factor also significantly affected all evaluated metabolites contents, except for tyrosine, essential (EAA) and branched-chain (BCAAs) amino acids. Over two years of field trial, FID biostimulant enhanced the contents of proteins (+53.71%), alanine (+16.55%), aspartic acid (+31.13%), γ-aminobutyric acid (+76.51%), glutamine (+55.17%), glycine (+28.13%), monoethano-lamine (+19.57%), total amino acids (+33.55), essential amino acids (+32.56%) and branched-chain amino acids (+45.10%) compared to the untreated control. Our findings highlighted the valuable effect of FID microbial inoculant in boosting several primary metabolites (proteins and amino acids) in the fruits of processing tomato crop grown under Southern Italy environmental condi-tions, although no effect on yield and its components was appreciated.

Disruption of the in utero environment can have dire consequences on fetal growth and development. Intrauterine growth restriction (IUGR) is a pathological condition by which the fetus deviates from its expected growth trajectory, resulting in low birth weight and impaired organ function. The developmental origins of health and disease (DOHaD) postulates that IUGR has lifelong consequences on offspring well-being, as human studies have established an inverse relationship between birth weight and long-term metabolic health. While these trends are apparent in epidemiological data, animal studies have been essential in defining the molecular mechanisms that contribute to this relationship. One such mechanism is cellular stress, a prominent underlying cause of the metabolic syndrome. As such, this review considers the role of oxidative stress, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, and inflammation in the pathogenesis of metabolic disease in IUGR offspring. In addition, we summarize how uncontrolled cellular stress can lead to programmed cell death within the metabolic organs of IUGR offspring.

Agricultural Production today has to deal with different challenges. It has to increment production for a continuously increasing population, reducing the environmental burdens on the natural systems. In conventional agriculture, this is possible through the increase of inputs, especially nutrients, which, however, are responsible for the biggest part of emissions. It becomes more complicated though, adopting sustainable agricultural practices, to improve the quality and the quantity of agricultural production reducing the inputs use. Plant growth regulators are described in the literature for the significant role in securing crop management of modern agriculture. Therefore, this joint field experiment has been carried out on a pear orchard (Pyrus communis L. cv. Abate Fètel) in Emilia Romagna (Italy) by Fondazione Navarra and TIMAC AGRO Italia S.p.A., to test the “less for more” theory which consists in getting more and better agricultural produce using fewer inputs. Preliminary results of two consecutive years have confirmed our assumption as it was possible to substantially reduce the total fertilisation units applied, improving significantly quantitative and qualitative production indicators (i.e. flower and fruit density, fruit set (%), the average weight of fruits (g) and the total yield (t/ha)). Results have also shown a positive correlation between plant growth regulators and agronomic efficiency of pears.

The present study was conducted to evaluate encapsulated essential oils as an alternative anticoccidial in coccidiosis vaccine challenged broiler chickens. A total of 600 day-old male broiler chicks were provided with no-added corn-soybean meal-based control diet or diets that contained either salinomycin or EO at 60 and 120 mg per kg of diet. On day 21, half of the control groups were orally challenged with a coccidiosis vaccine at 25 times higher than the recommended vaccine dose. During 22 to 28 days (i.e., one-week post coccidiosis vaccine challenge), the challenged chickens had decrease (P < 0.05) in body weight gain and feed intake but increase in feed conversion ratio compared with the non-challenged, naïve control chickens. However, dietary EO significantly counteracted (P < 0.05) coccidiosis vaccine-induced depression in body weight gain and feed intake. Increasing dietary EO linearly decreased (P < 0.05) the concentrations of the volatile fatty acids. Dietary SAL and EO affected gut morphology in chickens at 20 days posthatch. Increasing dietary EO linearly (P = 0.073) increased serum catalase activity. Collectively, our study shows that dietary EO increased coccidiosis vaccine-induced growth depression and altered gut physiology in broiler chickens.

In this work, the nearest neighbor distances and Voronoi cell features of the Cu-Ag deposits were analyzed and fitted with Lognormal, Weibull, and Gamma distributions. The nearest neighbor distance distributions of the samples were compared with those of complete spatially random points, showing spatial inhomogenity due to the nucleation exclusion effect. The radial distribution function was calculated, showing both influences from the grain size and the nucleation exclusion effect. Voronoi cells were generated based on the shape of the grains. The size, occupancy, and coordination of the Voronoi cells were examined and fitted. The results show that despite the Cu-Ag deposits seem to be governed by instantaneous nucleation mode, the spatial distribution of the nuclei is more impacted by the nucleation exclusion effect than the Cu-only samples. This behavior is also justified by the grain size distribution generated with Voronoi cell size and occupancy distributions.

Mammals have two insulin-like growth factors (IGF) that are key mediators of somatic growth, of tissue differentiation and cellular responses to stress. Thus, the mechanisms that regulate the bioavailability of IGFs are important in both normal and aberrant development. IGF-I levels are primarily controlled via the growth hormone-IGF axis, in response to nutritional status, and also reflect metabolic diseases and cancer. One mechanism that controls IGF bioavailablity is the binding of circulating IGF to a number of binding proteins that keep IGF in a stable, but receptor non-binding state. But even before IGF is released from the cells that produce it, it undergoes an obligatory association with a ubiquitous chaperone protein, GRP94. This binding is required for secretion of a properly folded, mature IGF. This chapter reviews the known aspects of the interaction and highlights the specificity issues yet to be determined. The IGF-GRP94 interaction provides a potential novel mechanism of idiopathic short stature, involving the obligatory chaperone and not just IGF gene expression. It also provides a novel target for cancer treatment, as GRP94 activity can be either inhibited or enhanced.

We recently reported that in the spinal cord of PPMS or SPMS patients, large areas of periplaque demyelinating lesions extend distance away from plaque borders. Such lesions are characterized by a progliotic TGF-beta 1 signature accompanied by: i) a low-grade inflammatory reaction, ii) an extensive astrocytosis and iii) a process of incomplete demyelination. It was proposed that, while efficiently dampening inflammation in MS spinal cords, TGF-beta 1 could promote astrocytosis, prevent remyelination and possibly trigger alterations of myelin synthesis. In light of these findings, a re-interpretation of two large neuropathological studies performed on MS brains and spinal cords is provided here. While results from these studies clearly showed that active plaques do not display any region-specific distribution, an important point was apparently overlooked and not discussed by the authors: a significantly higher percentage of inactive plaques was found in MS spinal cords as compared to brains and, conversely, the percentage of slowly-expanding (or smoldering) lesions was significantly lower in the spinal cord as compared to the brain. These data indicate that the spinal cord environment may be more favorable to the resolution of inflammation. Downstream of the autoimmune process leading to plaque formation, region-specific mechanisms may thus drive the outcome of active plaques. While inflammation triggers tissue destruction, inflammation may also be needed for effective tissue repair and an inappropriate dampening of inflammatory events may possibly translate into a poor level of remyelination in MS spinal cords. It is proposed here that TGF-beta 1 is involved in such a brain-spinal cord dissociation of active plaques outcome.

A feeding study was conducted to evaluate the different diets supplemented with vitamin solution effect on broodstock reproduction, growth performances, and the survival rates of larvae and fry of Pangasius catfish during over-spawning season. The experiment of broodstock cultivation was set up as a diet supplement design with six different diets consisting of vitamin solution, each diet fed to triplicate groups of fish, the stocking density of 10 broodstock fish (07 females and 03 males) per hapa net. Fish larvae and fry were reared in the hatchery for 45 days after hatching to evaluate growth performances and survival rates of fish fingerling. The growth and reproductive performances of breeders fed with different test diets showed that there were differences among the six tested diets (P < 0.05). The highest final body weight (FBW), weight gain (WG), daily weight gain (DWG), specific growth rate (SGR) of broodstock and survival rate of Pangasius fingerlings were found in Treatment 5, which contained 0.6% H-OVN for Pangasius Broodstock mixed with 12.6% algal oil and Treatment 3, which contained 0.6% vitamin premix H-OVN for Pangasius Broodstock compared to other Treatments. With these test diets, the Gonadosomatic index (GSI), relative fecundity index (RFI), fertilized egg, hatching rates of eggs, and survival rate of fingerling ranged between a 6.7–12.8%, 104,267–199,512 eggs/kg, 62.2–93.6%, 43.5–76.2%, and 45.3–66.3%, respectively (P < 0.05). The findings of this study showed that the diet containing 35% CP contents supplemented with 0.6% vitamin premix H-OVN mixed with algal oils showed the highest results in terms of growth, reproductive performance indices, and survival rates of Pangasius catfish fingerling.

The economic downturn caused by the financial crisis of 2008-2009 and the intensifying glob-al climate policy trends forced changes in the energy management of the European Union. The study examined how the most relevant energy economic variables affected the economic growth of the EU between 2010-2019. The study examined the relationship between GDP (dependent variable) and energy consumption, greenhouse gas emissions, the average energy price, and renewable energy use (independent variables) using the PSL-PM methodology. The results show an asymmetric picture because the independent variables influenced the eco-nomic growth of the EU in the examined period with different correlations and with different strengths. One of the significances of the study is that the presented and analyzed variables, as well as the results of the relationships between them, can contribute to the optimization of the EU's currently critical energy management and economic growth.

This study investigates the intricate relationship between Venezuelan migration and urban growth in Colombia from 2018 to 2021. The study employs remote sensing data and social network metrics to uncover migration patterns and their impact on urban expansion. The methodology consists of three stages. Firstly, nighttime satellite imagery is used to analyze year-over-year urban growth in Colombia. Secondly, social network data estimates Venezuelan migration, overcoming challenges of underreporting and informal border crossings. Lastly, an econometric analysis explores the quantitative link between Venezuelan migration and urban growth, integrating socioeconomic variables to address endogeneity. The findings reveal the complex interplay of Venezuelan migration, socioeconomic factors, and urban growth. The study outlines remote sensing analysis, introducing the Anthropogenic Footprint Expansion Index (AFEI) to quantify urban growth. Facebook API data estimates migration trends and explores socioeconomic impacts on urban expansion. The analysis uncovers migration, poverty, aging, and urban population proportion as key factors affecting Colombia's urban landscape. Furthermore, the research underscores how Venezuelan migration affected short-term urban expansion pre- and post-COVID-19. Migration had a notable effect before the pandemic, but this influence waned afterward. The study highlights migration's short-term nature and emphasizes age demographics' role in medium-term dynamics.

The present study investigated the insulin-like growth factors (IGFs) and their receptors and binding proteins among different pig breeds during weaning. Sixty Duroc (DR), Taoyuan black (TB), and Xiangcun black (XB) piglets (20 piglets per breed) were selected at 21 and 24 days of age (three days of post-weaning) to analyze organ indices, plasma concentrations of IGF and IGF-binding proteins (IGFBPs) using ELISA kits, and gene expression of IGF system-related components in different tissues. The plasma IGFBP-3 level in TB piglets was higher (P > 0.05) than in the XB and DR piglets at 21 days of age. At 21 days of age, compared with the DR piglets, the IGF-1 expression was lower (P < 0.05) in the kidney but was higher (P < 0.05) in the spleen of XB and TB piglets. At 24 days of age, the IGF-1 expression was higher (P < 0.05) in the kidney of TB piglets than in the XB and DR piglets, while IGFBP-3 in the stomach and IGFBP-4 in the liver of XB and TB piglets were lower (P < 0.05) compared with the DR piglets. Weaning down-regulated (P < 0.05) the IGF-1 expression in the jejunum, spleen, and liver of piglets, while up-regulated (P < 0.05) IGFBP-3 expression in the stomach, IGFBP-4 in the liver, IGFBP-5 in the ileum, and IGFBP-6 in the jejunum of DR piglets. Spearman’s correlation analysis showed a negative correlation (P < 0.05) between plasma IGFBP-2 and IGFBP-5 and the organ index of piglets. Collectively, there were significant differences in IGF system components among different pig breeds. The IGF system components were altered during weaning, which might be involved in weaning stress to decrease the growth of piglets.

Overuse and misuse of antibiotics can pose the risks of spreading mutant strains that show antimicrobial resistance (AMR), with negative impact on management of bacterial infections and economic implications on healthcare systems. Research and development of natural antibacterial agents could be a priority in the next years to improve number of effective antibacterial molecules and to reduce AMR phenomenon and its development. The present study identified the most effective concentration and contact time of Thymus vulgaris L. essential oil (TEO) to obtain bactericidal effect in vitro against different Gram + and Gram – bacterial strains. Six clinically isolated (wild types) bacterial strains, (Citrobacter freundii, Enterococcus feciorum, Proteus mirabilis, Acinetobacter cioffi, Pasteurella putrefaciens, Klebsiella pneumoniae) and two ATCC (Staphylococcus aureus and Streptococcus mutans) were tested after 1min, 3min and 5min of contact with TEO. Preliminary results on S. aureus after 24h incubation revealed a TEO concentration of 9.28mg/mL (w/v) that completely inhibited bacteria growth keeping cell viability. Total suppression of bacterial growth at all tested contact times was observed for all tested bacterial strains and the results were confirmed after 48h incubation. Bacterial growth suppression was confirmed even with presence of organic component. These preliminary results showed in vitro antimicrobial efficacy of TEO against different Gram+ and Gram- bacterial strains. Future studies are necessary to confirm the reproducibility of these results even on other strains and to define exactly the molecular mechanisms of EOs, in order to consider TEO as a valid alternative of classic antibiotic therapies and subsequently to reduce the occurrence of AMR.

In the application of classical nucleation theory (CNT) and all other theoretical models of crystallization of liquids and glasses it is always assumed that nucleation proceeds only after the supercooled liquid or the glass have completed structural relaxation processes towards the metastable equilibrium state. Only employing such assumption, the thermodynamic driving force of crystallization and the surface tension can be determined in the way it is commonly performed. The present paper is devoted to the theoretical treatment of a different situation when nucleation proceeds concomitantly with structural relaxation. To treat the nucleation kinetics theoretically for such cases, we need adequate expressions for the thermodynamic driving force and the surface tension accounting for the contributions caused by the deviation of the supercooled liquid from metastable equilibrium. In the present paper, such relations are derived. They are expressed via deviations of structural order parameters from their equilibrium values. Relaxation processes result in changes of the structural order parameters with time. As a consequence, the thermodynamic driving force and surface tension, and basic characteristics of crystal nucleation, such as the work of critical cluster formation and the steady-state nucleation rate, also become time-dependent. We show that this scenario may be realized in the vicinity and below the glass transition temperature, and it may occur only if diffusion (controlling nucleation) and viscosity (controlling the alpha-relaxation process) in the liquid decouple. Analytical estimates are illustrated and confirmed by numerical computations for a model system. The theory is successfully applied to the interpretation of experimental data. Several further consequences of this newly developed theoretical treatment are discussed in detail. In line with our previous investigations, we reconfirm that only when the characteristic times of structural relaxation are of similar order of magnitude or longer than the characteristic times of crystal nucleation, elastic stresses evolving in nucleation may significantly affect this process. Completing the analysis of elastic stress effects, for the first time expressions are derived for the dependence of the surface tension of critical crystallites on elastic stresses. As the result, a comprehensive theoretical description of crystal nucleation accounting appropriately for the effects of deviations of the liquid from the metastable states and of relaxation on crystal nucleation of glass-forming liquids, including the effect of simultaneous stress evolution and stress relaxation on nucleation, is now available. As one if its applications, this theoretical treatment provides a new tool for the explanation of the low-temperature anomaly in nucleation in silicate and polymer glasses (the so-called \breakdown" of CNT at temperatures below the temperature of the maximum steady-state nucleation rate). We show that this anomaly results from much more complex features of crystal nucleation in glasses caused by deviations from metastable equilibrium (resulting in changes of the thermodynamic driving force, the surface tension, and the work of critical cluster formation, in the necessity to account of structural relaxation and stress effects) than assumed so far.

Ulmus species have been widely used in Korean folk medicine because of their anti-inflammatory and antimicrobial properties. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of Ulmus parvifolia (UP) in skin and skin wound healing effect using keratinocyte model. in vivo experiments showed that the wound lesions in the mice decreased by U. parvifolia with 200 mesh size of root bark powder and significantly reduced by treatment with UP, compared with those treated with U. macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder of exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. TGF-β In, Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvernation.

Field experiments were carried out during 2011-2014 at the Research and Experimental Center to Combating Desertification located in the Elsen Tasarkhai station of central Mongolia. The study was aimed to identify the effects of oxidized brown coal humic acid fertilizer on the relative growth rate of several trees for forest rehabilitation. The trees used were Populus sibirica, Salix ledebouriana and Acer tataricum. The experiment was carried out with the concentrations of 2,000, 10,000 and 20,000 mg L-1 of humic acid fertilization treatments. The measurement of relative height growth rate (RHGR) was conducted for a period of 4 years. The treatments showed significant differences within humic fertilizer concentrations which differed depending on the species. Compared to the monthly RHGR over the study, the treatment of fertilizers were significantly better for tree growth. P. sibirica in the 10,000 and 20,000 mg L-1 humic acid fertilizers had significant height growth rates. In addition, when the humic acid treatments were compared to control, A. tataricum decreased over the years which were statistically significant for high growth rate and a positive effect of humic acid fertilization treatments was observed. Furthermore, results showed that oxidized brown coal humic acid fertilizers as organic fertilizer can have a positive effect for the growth of A. tataricum during the study years. The results showed that the soil chemical properties EC, CO2, NO3, and K2O were significant among all the treatments compared to the control. The effect on P2O5 statistically significantly increased among all the treatments. However, pH and Mg were not significant effect among all the treatments. Combining the results obtained from the oxidized brown coal humic acid fertilization with sustainable land management practices can help improve soil organics for environmental issues in degraded sandy soil regions.

Insulin-like Growth Factor 2 mRNA binding Protein 1 (IGF2BP1) is a promising candidate gene for influencing economic traits in livestock and poultry. The second intronic region of IGF2BP1 is closely related to growth traits, although the exact mechanism remains unclear. The preliminary resequencing results of goats in our laboratory demonstrated that the IGF2BP1 gene region was subject to strong selection, then we selected multiple single nucleotide polymorphisms (SNPs) to identify. Seven SNPs were located in the conserved region of the second intron, and their functions and relationship with growth traits require further clarification. This study selected 348 Nanjiang Yellow goats as experimental subjects, and chose Time of Flight Mass Spectrometer (MALDI-TOF) as the main method. Our results showed that six SNPs were significantly correlated with the growth traits of the Nanjiang yellow goat by association analysis. Among them, the rs652062749(A>G) was identified as the primary locus affecting the later stage of growth traits of Nanjiang yellow goat. Moreover, there was a strong linkage among three SNPs, and the rs638185407 (T>A) mutant type was observed to increase the luciferase activity in H293T cells extremely significantly. The TTAACT, TTCCCC, and ATCACT combination genotypes were dominant combination genotypes, which provided theoretical references for further genetic co-breeding. The results of our study demonstrate the potential role of the IGF2BP1 gene as a functional genetic marker in the breeding programs of Nanjiang Yellow goats.

Marine sediment sample was collected and the total aerobic population was counted and probiotic Bacillus cereus was isolated. Then it was mixed with formulated feed by spraying, while control diet was purchased from a local aquarium. 30 days of feeding experiment was conducted. Carassius auratus fishes were randomly divided into two experimental groups in rectangular tanks. The first group served as a control in which fishes were fed with commercial feed. The second group of fish were fed with probiotic incorporated feed. After 40 days, their survival and growth performance were detected. Bacillus cereus incorporated feed significantly yielded higher survival rate of the fish compared to control. Food conversion ratio (FCR) was significantly lower than the control group. The main aim of this work is to investigate the effect of a marine probiotic bacteria, Bacillus cereus on the survival and growth performance of gold fish Carassius auratus.

It is required to manage sustainable Short-Rotation Coppices (SRCs) as an important role on carbon sink and bioenergy output, because most of SRCs were established in reclaimed land in South Korea. However, during the last three years, growth pattern of the SRCs was remarkably changed with soil condition. This study aimed to identify the sustainability of SRCs on carbon storage, biomass and fuel pellet production, monitoring the neighboring vegetation of SRCs by land-use exchange, physiological change of poplar on seasonal trend, and to evaluate whether poplar is suitable for making wood pellets. The calculated biomass yield per area of poplar grown was 103.07 Mg per total area (55.6 ha), and volumes of carbon dioxide absorption was estimated to be 330 Mg CO2. Wood pellet quality based on the criteria scored third grade, indicating that poplar is suitable for manufacturing fuel pellets. Moreover, monitoring of the flora distribution in SRCs revealed changes in species composition. As halophyte was increased during drought, soil organic matter, net growth and total chlorophyll of poplar were significantly decreased. These findings indicated that photosynthesis and growth pattern of SRCs may be negatively affected by microclimate and will provide valuable information for effective management of SRCs.

This paper presents a comprehensive investigation into the applicability and performance of two prominent growth models, namely the Verhulst model and the Montroll model, in the context of modeling tumor cell growth dynamics. Leveraging the power of Physics-Informed Neural Networks (PINNs), we aim to assess and compare the predictive capabilities of these models against experimental data obtained from the growth patterns of tumor cells. We employ a dataset comprising detailed measurements of tumor cell growth to train and evaluate the Verhulst and Montroll models. By integrating PINNs, we not only account for experimental noise but also embed physical insights into the learning process, enabling the models to capture the underlying mechanisms governing tumor cell growth. Our findings reveal the strengths and limitations of each growth model in accurately representing tumor cell proliferation dynamics. Furthermore, the study sheds light on the impact of incorporating physics-informed constraints on the model predictions. The insights gained from this comparative analysis contribute to advancing our understanding of growth models and their applications in predicting complex biological phenomena, particularly in the realm of tumor cell proliferation.

In recent years, long periods of drought and heat waves have become increasingly frequent, causing forest dieback phenomena that make stands more sensitive to biotic stressors. How trees may respond to extreme climatic events and which metabolites are involved under stress conditions is still not clear. In this study, we analysed by SPME how dieback (D) and non-dieback (ND) Hungarian oak trees from the San Paolo Albanese site respond to these climatic dynamics, in terms of volatile organic compounds (VOCs). For each group of trees, three wood samples were taken, each divided into four sub-samples with 5 growth rings, subjected to SPME and increase in basal area (BAI) analysis of the last 20 years. Dieback trees had a lower amount of leaves, and this condition may translate into less photosynthesis, less organic matter production and lower reserves of carbohydrates available for growth. Indeed, D trees showed lower radial increases and a lower content of aldehydes, terpenes, and fatty acids than ND trees, indicating a better health of ND trees compared to D trees. While D trees showed a reduction in terpenes, such as α-pinene, γ-eudesmol, and cyperene (with significant insecticidal activity), a reduction in aromatic aldehydes, such as furfural and 5-methylfurfural, and an increase in silanols (with antimicrobial function). Considering the different compounds’ contents between D and ND trees, our study could be useful for detecting bio-indicators to identify an early warning signal of dieback phenomena.

The study presents empirical results investigating the relationships among renewable and non-renewable energy consumption, CO2 emissions, and GDP within the Visegrád Group (V4) countries. Using FMOLS/DOLS and ARDL approaches, along with causality tests based on the Toda-Yamamoto method, the study explores these relationships at a regional level. The findings indicate that renewable energy has a small positive impact on long-term economic growth, with non-renewable energy having a more significant effect. Moreover, CO2 emissions have a negative impact on economic growth, suggesting ongoing reliance on non-renewable energy sources and a burden on economic expansion. On an individual country level, the effects vary. Poland, Slovakia, and Hungary exhibit a negative relationship between CO2 emissions and economic growth. Energy sources also differ in impact: in Poland, the Czech Republic, and Slovakia, non-renewable energy significantly affects economic growth, while in Hungary, renewable energy plays a more substantial role. Causality tests reveal a causal relationship between CO2 emissions and economic growth in the Czech Republic and Poland, suggesting CO2 emissions significantly influence economic expansion. In terms of energy production, renewable energy is causally related to economic growth in the Czech Republic and Slovakia. All countries demonstrate significant causality between non-renewable energy and economic growth. Additionally, a relationship between renewable energy and CO2 emissions is confirmed in Poland.

Traumatic brain injury represents one of the main health problems in developed countries. Growth Hormone (GH) and rehabilitation have been claimed to significantly contribute to the recovery of lost motor function after acquired brain injury, but the mechanisms by which this occurs are not well understood. In this work, we have investigated cell proliferation in the piriform cortex (PC) of adult rats with ablation of the frontal motor cortex treated with GH and rehabilitation, in order to evaluate if this region of the brain, related to the sense of smell, could be involved in benefits of GH treatment. Male rats were either ablated the frontal motor cortex in the dominant hemisphere or sham-operated and treated with GH or vehicle at 35 days post-injury (dpi) for five days. At 36 dpi, all rats received daily injections of bromodeoxyuridine (BrdU) for 4 days. We assessed motor function through the paw-reaching-for-food task. GH treatment and rehabilitation at 35 dpi significantly improved the motor deficit caused by the injury and promoted an increase of cell proliferation in the PC ipsilateral to the injury, which could be involved in the improvement observed. Cortical ablation promoted greater number of BrdU+ cells in the piriform cortex that was maintained long-term, which could be involved in the compensatory mechanisms of the brain after injury.

Regeneration is crucial for forest continuity in natural and managed stands. Analyzing intra-annual dynamics improves the understanding between growth and climate, identifying survival thresholds. The objective was to determine microclimate constraints (rainfall, air and soil temperatures) over Nothofagus antarctica regeneration growing at closed, open, and edge forests in Patagonia. We measured stand characteristics (forest structure, understory, soil properties, animal use), microclimate, and daily growth of regeneration using dendrometers (n = 6) during two growing seasons. We found significant differences in the studied variables (e.g. overstory, light, soil, understory, animal use). These changes defined microclimate across overstory gradient (e.g. soil moisture), influencing the daily growth across seasons (lag, exponential, stationary). Rainfall influenced more than temperature. Daily growth in closed forests indicating shrinkage (-0.0082 mm day-1 without rain, and -0.0008 mm day-1 with 0.0-0.2 mm day-1 rainfall), while above 0.2 mm day-1 rainfall, growth always increased. Open forests presented shrinkage during days without rain (-0.0051 mm day-1), showing positive growths according rainfall. Edge forests always presented positive daily growths. The resilience of regeneration under these changed conditions was related with overstory. Main outputs indicated that regeneration was vulnerable during non-rainy days, suggesting the needs of long-term monitoring to develop better silvicultural proposals.

Increasing numbers of researchers are interested in the importance of dietary fiber for the gut microbiota, microbiotal metabolite SCFA, energy metabolism and gut health of the host. However, studies have demonstrated that long-term and longitudinal observation may be needed to evaluate the effect of dietary fiber better, and few such works have been made in chickens. Therefore, we successively fed low-fiber, high--fiber and low-fiber diets to two breeds of chickens during different growth periods (1—8, 9—20 and 21—50 weeks), aiming to longitudinally observe the long-term effect of altered dietary fiber on the gut microbiota, SCFA and development of cecum of chickens with age. The results showed that the composition and function of the gut microbiota, SCFA and the development of the cecum were different during different periods, which was largly affected by dietary fiber. However, the causes of some effects were different during the different periods. For example, compared with that in low-fiber chickens at 8 weeks, dominant fiber-degradation bacteria such as Bacteroidetes, Alloprevotella and SCFA-producing bacteria such as Faecalibacterium increased due to a high-fiber diet at 20 weeks, while due to a high feed intake in 50 weeks. Moreover, the concentration of SCFA in 20 weeks was significantly higher than in 8 weeks and 50 weeks, but the causes of this difference were also distinct. It was proposed that a long-term observation was needed to evaluate the effect of dietary fiber better on chickens. The metabolite pathways of ATP-binding cassette (ABC) transporters encoded by Firmicutes were enriched in 8 weeks, while a two-component system and β–glucosidase encoded by Bacteroidetes were enriched in 20 and 50 weeks. The trend was the same in two breeds of chickens except for Alloprevotella. In addition, the total content of SCFA in the contents of cecum was also affected by the size of the cecum. Surprisingly, the length of the cecum shortened from 20—50 weeks, maybe due to reduced dietary fiber.

The rapid growth of urban areas is a major challenge facing cities around the world. This growth can have a significant impact on the local climate, leading to higher temperatures and other changes. In desert climates, the effects of urban expansion can be particularly pronounced. This study investigated the impact of urban expansion on land surface temperature (LST) in Baghdad, Iraq. Notably, this study employs a sophisticated artificial intelligence method known as Random Forest for Land Use Land Cover (LULC) classification, utilizing three Landsat images spanning the temporal spectrum from 1985 to 2021 to meticulously monitor land use transformations and associated LST variations. The results showed that vegetated areas declined by 46.8% during the study period, while built-up areas increased by 124.7%. This decline in vegetation was accompanied by an increase in LST, with bare soil recording the highest temperatures. The study also found that LST has a strong inverse relationship with vegetation and moisture. This means that areas with more vegetation and moisture tend to have lower LSTs. These findings suggest that urban expansion can lead to higher LSTs in desert climates, which can have implications for the health and wellbeing of residents. The study has important implications for urban planners and policymakers in Baghdad and other cities in desert climates. By identifying the main factors that control LST, the study provides insights into strategies for mitigating the effects of urban expansion on temperature.

During chronic liver injury, inflammation leads to liver fibrosis— particularly due to the activation of hepatic stellate cells (HSCs). However, the involvement of inflammatory cytokines in HSC activation and the relationship among different liver cells is unclear. To examine their interactions, many in vitro liver models are performed in organoid or spheroid culture with random 3D structure, complicating analysis. Herein, we demonstrated the hierarchical coculture of primary rat hepatocytes with non-parenchymal cells such as the human-derived HSC line (LX-2) and liver sinusoidal endothelial cell line (TMNK-1). The cocultured tissue had high usability with simple operation of separating solid and liquid phases with improved liver functions such as albumin production and hepatic cytochrome P450 3A4 activity. We also studied the effects of stimulation by both oxygen tension and the key pro-fibrogenic cytokine, transforming growth factor beta (TGF-β), on HSC activation. Gene expression analysis revealed that lower oxygen tension and TGF-β1 stimulation enhanced collagen type I and alpha-smooth muscle actin expression from LX-2 cells in the hierarchical coculture. Therefore, this hierarchical in vitro cocultured liver tissue could provide an improved platform as a disease model for elucidating the interactions of various liver cell types and biochemical signals in future liver fibrogenesis studies.

Changing climate conditions and rapidly increasing carbon dioxide emissions have severely affected the global ecosystem and world economy. Governments around the world have paid attention to these resulting challenges and enacted many policies to reduce environmental pollution and carbon dioxide (CO2) emissions. This study aimed to determine and understand the relationship between innovation, foreign direct investment, economic growth, renewable consumption and CO2 emission in Vietnam. Information was collected annually based on the annual data of the General Statistics Office of Vietnam and World Bank from 2000 to 2022. Data processing was conducted using the STATA 17.0 software. The innovation affects Vietnam’s environmental pollution. Innovation positively affect environmental pollution in Vietnam; if Vietnam’s innovation were to increase by 1%, CO2 emissions would increase by 0.68%. The empirical research results of this study also show that renewable energy consumption has a negative effect on environmental pollution in Vietnam; if renewable energy were consumed at a 1% increase, CO2 emission would decrease by 0.51%. In addition, FDI inflows and economic growth have strongly positive affecting to environmental pollution; if Vietnam’s FDI inflows were to increase 1%, CO2 emissions would increase 1.39%; if Vietnam’s GDP increase 1% then CO2 emission would increase 1.26%. This paper also provides some recommendations that can assist Vietnam in developing a green and sustainable economy in the technology revolution 4.0 to achieve the United Nations Sustainable Development Goals (SDGs) over a long-term period.

Taking Shanxi Province, a typical energy region in China, as the research object, data on economic development quality and environmental pollution intensity from 2001 to 2021 were selected. Linear regression, numerical simulation, and Pearson correlation coefficient were used to analyze the evolution characteristics of economic development quality and environmental pollution intensity in Shanxi Province in the past 20 years, and to explore the relationship between economic development and environmental pollution. The results indicate that since 2001, Shanxi Province has made long-term progress in economic development, with GDP increasing by nearly 10 times and an average annual growth rate of around 7%. In the past 20 years, the main pollutants have shown a trend of increasing first and then decreasing steadily, with a turning point occurring during the "12th Five Year Plan" period. This shows that the environmental policies and investments of the Chinese and Shanxi provincial governments in the past 10 years have been effective in the new era. The numerical simulation curve results show that the per capita GDP exhibits a classic inverted "U" curve relationship with wastewater and SO2 emissions, with a turning point occurring at around 20000 yuan per capita GDP; However, there is a monotonic decreasing trend with Chemical oxygen demand and ammonia nitrogen emissions, and a monotonic increasing trend with solid waste generation. There is no turning point yet. The correlation analysis results further support the fitting curve conclusion.

Entomopathogenic fungi perform important functions in the ecosystem as natural antagonists of insects, which can be used in agriculture. Interestingly, recent studies showed a significant promotion of tested plants growth in the presence of fungi. We hypothesize that some of various compounds produced by entomopathogenic fungi can positively affect plant development. To test this hypothesis, fungal extracts of entomopathogenic fungus Beauveria bassiana were prepared at different conditions of temperature and pH. In addition to determination of the ammonium nitrogen content, the composition of extracts was analyzed by elemental ICP-OES. Then, their effect on the wheat germ growth was studied using various extract concentrations. After experiments, tested plants were measured, weighed, and the chlorophyll content was determined. Finally, the impact of extracts on the selected G+ and G- bacteria growth was examined to exclude the possibility of interference with soil microorganisms. The highest length of the wheat shoot was obtained for the use of 10-times diluted extract (10%) at pH 10 obtained at 20°C. In contrast, addition of 10% extract (pH 10) obtained at 75°C resulted in the shortest shoot. Generally, the extracts obtained at 75°C showed phytotoxic properties leading to lower values of shoot length and fresh weight in comparison to the control group. Our preliminary results are the first confirming the potential of fungal water extracts as factors promoting plant growth. Further detailed study should be carried out to confirm the effects in real environment conditions. Also, the consistency of the plant growth stimulation across different entomopathogenic fungi, and agriculturally used plant species should be tested.

This paper reports the effect of silicon substrate orientation and aluminum nitride buffer layer deposited by molecular beam epitaxy on the growth of aluminum nitride thin films deposited by DC magnetron sputtering technique at low temperature. The structural analysis has revealed a strong (0001) fiber texture for both substrates Si (100) and (111) and a hetero-epitaxial growth on few nanometers AlN buffer layer grown by MBE on Si (111) substrate. SEM images and XRD characterization have shown an enhancement in AlN crystallinity thanks to AlN (MBE) buffer layer. Raman spectroscopy indicated that the AlN film was relaxed when it deposited on Si (111), in compression on Si (100) and under tension on AlN buffer layer grown by MBE/Si (111) substrates, respectively. The interface between Si (111) and AlN grown by MBE is abrupt and well defined; contrary to the interface between AlN deposited using PVD and AlN grown by MBE. Nevertheless, AlN hetero-epitaxial growth was obtained at low temperature (<250°C).

Poverty alleviation must be carried out in a planned and sustainable manner by the central government and regional governments, the private sector, and the entire community to achieve a prosperous society by getting special attention from the government. This study aims to analyze the impact of the non-cash food assistance program on poverty alleviation, stunting and economic problems using a public private partnership (PPP) approach that involves the role of the government, the private sector and the community. This study uses a qualitative-explorative research method to examine Indonesia's non-cash food assistance program as a national initiative to fight poverty in the form of food. Data were collected from interviews, field observations, and relevant literature, then Nvivo 12 Pro was used to analyze the results. The location of this research is in Indonesia, namely Takalar Regency. The results of the study show that the implementation of the non-cash basic food assistance program has been effective. The involvement of the government, the private sector, and the community shows this. The success factors for the basic food assistance program are alleviating poverty in Indonesia, reducing stunting rates, and increasing economic growth because people are given a place to be part of small businesses. From the results of this analysis it was concluded that the non-cash basic food assistance program is a special program that can overcome various social problems in society. So that poverty alleviation programs are expected to be sustainable and improved to provide benefits for people's welfare. The implementation of the food aid program also involved several cross-sectoral efforts to channel government assistance.

The power of movement in plants” by Charles Darwin details many examples of oscillatory growth exemplified more recently at the single cell level by pollen tube tip oscillations and associated ion fluxes, particularly Ca²⁺and H⁺. That implies an underlying growth oscillator based on the recent discovery that classical AGPs bind Ca²⁺ at the cell surface. The juxtaposition of AGPs with three other components embedded in the plasma membrane is evidence of a Ca²⁺ cycle that generates cytosolic Ca²⁺. It involves Ca²⁺ channels, auxin efflux “PIN” proteins and an auxin-activated proton pump that dissociates AGP-Ca²⁺ on demand. While the apparent simplicity of this system satisfies Occam’s Razor its hypothetical role as a global growth oscillator demands rigorous experimental appraisal. The wide ramifications extend from pollen tubes to stomatal guard cells. Stomata act as crucial components of a water hypercycle that contributes to homeostasis of a warming planet by regulating evaporative cooling, reflective cloud cover generated by vast forests and the high albedo of snow-capped mountains including polar ice-caps . The Gaia hypothesis of James Lovelock and Lynn Margulis is a brilliant metaphor.

Compact solar-powered plasma water generator: enhanced on-site aged seed germination with the corona dielectric barrier discharger

Breast cancer is a multifaceted disease that has many subcategories characterized by unique genetic features. This research focuses on two important subgroups, including ER+ and HER2-. We conducted an analysis of gene expression data obtained from reliable sources (Array Express: E-GEOD-52194, E-GEOD-75367, and E-GEOD-58135) in order to reveal the complex molecular details of these subtypes. The computational pipeline we used identified 396 genes that exhibited distinct patterns of gene expression in ER+ and HER2- breast cancers. The diagnostic and prognostic significance of these genes was evaluated using machine learning methods, namely SVM and decision tree models. Metrics like as accuracy, sensitivity, and specificity provide insights into their usefulness. Furthermore, the use of the STRING database for network analysis revealed significant signaling pathways and biological processes associated with the development of ER+ and HER2- breast cancer. The results of our research enhance our comprehension of these subcategories, which might possibly facilitate more accurate diagnoses and focused treatment interventions. To summarize, this work provides valuable information on the genetic foundations of ER+ and HER2- breast cancer, which has potential implications for enhancing patient treatment and outcomes.

Intrauterine growth restriction (IUGR) pigs are characterized by long-term growth failure, metabolic disorders, and intestinal microbiota imbalance. The characteristics of the negative effects of IUGR at different swine growth stages are still unclear. Therefore, this study explored whether intrauterine growth restriction (IUGR) model damaged the intestinal barrier function and altered the colonization and metabolic profiles of the colonic microbiota in growing-finishing pigs by the multi-omics method. Seventy-two piglets (36 IUGR and 36 NBW) were selected for this trial to analyze physiological and plasma biochemical parameters, oxidative damage, and inflammatory response in the colon. Moreover, the colonic microbial communities were examined using 16s rRNA sequencing and metabolomics to reveal the intestinal characteristics of pigs at different growth stages (25, 50, and 100 kg). IUGR altered the plasma concentrations of glucose, total protein triglyceride, and cholesterol. Colonic tight junction proteins were markedly inhibited by IUGR. IUGR decreased plasma T-AOC, SOD, and GSH levels, and colonic SOD-1, SOD-2, and GPX4 expressions by restraining the Nrf2/Keap1 signaling pathway. Moreover, IUGR increased the colonic IL-1β and TNF-α levels while reducing IL-10, possibly activating the TLR4-NF-κB/ERK pathways. Notably, IUGR pigs had lower colonic Streptococcus abundance and Firmicutes-to-Bacteroidetes ratio at the 25 kg BW stage while having higher Firmicutes abundance at the 100 kg BW stage; moreover, IUGR pigs had lower SCFAs concentrations. Metabolomics analysis showed that IUGR increased colonic lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic concentrations and enriched three differential metabolic pathways, including linoleic acid, sphingolipid, and purine metabolisms throughout the trial. Collectively, IUGR altered the nutrient metabolism, redox status, and colonic microbial community and metabolite profiles of pigs and continued to disrupt colonic barrier function by reducing antioxidant capacity via the Nrf2/Keap1 pathway and activating inflammation via the TLR4-NF-κB/ERK pathway during the growing-finishing stage. Colonic Firmicutes and Streptococcus could be potential regulatory targets for modulating the metabolism and health of IUGR pigs.

The ADP-ribosylation factor 6 (Arf6), as the only member of the Arf family III protein, has been extensively studied for its diverse biological functions in animals. Previously, the Arf6 protein in Magnaporthe oryzae was found to be crucial for endocytosis and polarity establishment during asexual development. However, its role remains unclear in Sclerotinia sclerotiorum. Here, we identified and characterized SsArf6 in S. sclerotiorum using a reverse genetic approach. Deletion of SsArf6 impaired hyphal growth and development, and produced more branches. Interestingly, knockout of SsArf6 enhanced the tolerance of S. sclerotiorum to oxidative stress but the sensitivity to osmotic stress, and increased melanin accumulation, indicative of different roles of SsArf6 in various stress responses. Moreover, the appressorium formation was severely impaired, and fungal virulence to host plants was significantly reduced. Overall, our findings demonstrate the essential role of SsArf6 in hyphal development, stress responses, appressorium formation, and fungal virulence to host plants.

Aims: This systematic review aims to evaluate the influence of Environmental Enrichment (EE) on oncological factors in experimental studies involving various types of cancer models. Methods: A comprehensive search was conducted in three databases: PubMed (161 articles), Embase (335 articles), and Scopus (274 articles). Eligibility criteria were applied based on the PICOS strategy to minimize bias. Two independent researchers performed the searches, with a third participant resolving any discrepancies. The selected articles were analyzed, and data regarding sample characteristics and EE protocols were extracted. The outcomes focused solely on cancer and tumor-related parameters, including cancer type, description of the cancer model, angiogenesis, tumor occurrence, volume, weight, mice with tumors, and tumor inhibition rate. Key-findings: A total of 770 articles were identified across the three databases, with 12 studies meeting the inclusion criteria for this systematic review. The findings demonstrated that different EE protocols were effective in significantly reducing various aspects of tumor growth and development, such as angiogenesis, volume, weight, and the number of mice with tumors. Furthermore, EE enhanced the rate of tumor inhibition in mouse cancer models. Findings: This systematic review highlights the significant impact of EE protocols on multiple parameters associated with tumor growth and development, including angiogenesis, occurrence, volume, weight, and tumor incidence. Moreover, EE demonstrated the potential to increase the rate of tumor inhibition. These findings underscore the importance of EE as a valuable tool in the management of cancer.

When we discuss migration and compare national trends, the topic most commonly centers on undocumented immigrants and asylum seekers. However, there is also considerable variation in internal migration patterns, with differences found between and within nations. Many factors shape whether, why, and where people relocate. Over the last few decades, the world's two largest economies - the United States and China - have seen domestic migration changes happening in opposite directions. China has become increasingly mobile and experienced urbanization. In contrast, the U.S. has seen a steady decline in the rate at which its population relocates, with cities experiencing net migration losses as citizens leave for suburbs. This paper examines these trends using data from the last decade to compare the two nations in areas of internal migration, urbanization, housing, social mobility, and economic growth.

Soil-borne beneficial microbes establish symbioses with plant hosts, and play key roles during growth and development therein. In this study, two fungal strains, FLP7 and B9, were isolated from the rhizosphere microbiome associated with Choy Sum (Brassica rapa var. parachinensis) and barley (Hordeum vulgare), respectively. Sequence analyses of the internal transcribed spacer and 18S ribosomal RNA genes combined with colony and conidial morphology identified FLP7 and B9 to be Penicillium citrinum strains/isolates. Plant-fungus interaction assays revealed that isolate B9 showed significant growth promotion effects in Choy Sum plants cultivated in normal soil, as well as under phosphate-limiting conditions. In comparison to the mock control, B9-inoculated plants showed a 34% increase in growth in aerial parts, and an 85% upsurge in the fresh weight of roots when cultivated in sterilized soil. The dry biomass of such fungus-inoculated Choy Sum increased by 39% and 74% for the shoots and roots, respectively. Root colonization assays showed that P. citrinum associates directly with the root surface but does not enter or invade the root cortex of the inoculated Choy Sum plants. Preliminary results also indicated that P. citrinum can promote growth in Choy Sum via volatile metabolites too. Interestingly, we detected relatively higher amounts of gibberellins and cytokinins in axenic P. citrinum culture filtrates through liquid-chromatography mass-spectrometry analyses. This could plausibly explain the overall growth induction in P. citrinum-inoculated Choy Sum plants. Furthermore, the phenotypic growth defects associated with the Arabidopsis ga1 mutant could be chemically complemented by the exogenous application of P. citrinum culture filtrate, which also showed accumulation of fungus-derived active gibberellins. Our study underscores the importance of transkingdom beneficial effects of such mycobiome-assisted nutrient assimilation, and beneficial fungus-derived phytohormone-like metabolites in induction of robust growth in urban farmed crops.

Life sciences are increasingly benefiting from multidisciplinary research, combining biology, chemistry and physics. At their intersection, biothermodynamics applies the quantitative framework of thermodynamics to life processes and is a potentially interesting subject for life science students. Here a method is proposed to teach undergraduate life science students the basics of biothermodynamics, building and expanding on the concept of Gibbs energy learned as a part of the physical or general chemistry course. The discussion begins with the role of Gibbs energy as the driving force of metabolism and growth. Moreover, a biological example is proposed of the enthalpy-entropy combinations resulting in negative Gibbs energy, which should be interesting to life science students. Gibbs energy is then used to explain microorganism growth rates, using a simple thermodynamic growth model. Finally, multiplication of viruses is considered, including SARS-CoV-2, using Gibbs energy to explain the hijacking of host cell metabolism. Implementation of the proposed biothermodynamics material in classroom is discussed. The paper should be useful as material for making lectures for undergraduate students and as a starting point for anyone beginning to do research in biothermodynamics.

Objectives: The promising specific growth regulators are employed tissue culture of the bamboo species. The specific natural hardening mixture supports the acclimatization and adaptation of the protected cultivation. The bamboo species contribute to the carbon sequestration and stabilization of the environment. Findings: The growth regulator 2, 4-D encourages callus induction and growth regulators NAA + TDZ, BAP + Kinetin + Gelrite, BAP + Kinetin + Coconut water + myo-inositol, NAA and TDZ impose plant regeneration in the Bambusa species. The growth regulator of 2,4-D, 2,4-D + Kinetin, 2,4-D + BA, 2,4-D + BAP and 2,4-D + NAA + BAP produces callus induction in the Dendrocalamus species. The growth regulator of TDZ, BA and IBA + Coumarin + Putrescine, BAP + GA3, NAA + Kinetin, BA + IBA, BA + Kinetin + NAA, NAA + Kinetin, Kinetin + IAA has facilitated shoot regeneration in the Dendrocalamus species with the specific period. The growth regulator of BA and TDZ; BAP + TDZ, IBA + Coumarin + Putrescine, BAP + GA3, NAA + Kinetin, BA + IBA, BA + Kinetin + NAA, NAA + Kinetin, Kinetin + IAA develops root regeneration with unambiguous time in the Dendrocalamus species. The growth regulator of BAP and Kinetin + BAP imposes shoot regeneration and IBA and IBA + Coumarin + sucrose conducts root regeneration in the edible bamboo. The natural hardening materials of cocopeat, vermicompost, perlite, cow dung, FYM, compost, soil & garden soil and humus soil, perlite, and FYM recommend in the acclimatization and adaptation of the Bambusa species and Dendrocalamus species respectively. Novelty: The standard growth regulators and hardening mixtures impose tissue culture, acclimatization and adaptation in the bamboo species. The bamboo species involves in managing carbon sequestration, biogeochemical cycle and the environment.

In perennial fruit crops, bearing can be influenced by various factors, including environmental conditions, germplasm, rootstocks, and cultivation methods. Cherries, one of the most important and popular fruit species from the temperate climate zone, achieve high prices on the market. New agricultural technologies and environmental factors force a change in the approach to cherry cultivation. Old-type cherry orchards with their high demand for water, nutrients and manual work are replaced by orchards of self-pollinating cherry cultivars grown on dwarf rootstocks. These changes make it necessary to search for ways to regulate fruiting, in particular to thin buds, flower and fruit. In view of environmental regulations and consumer pressure, thinning methods are being sought that either do not involve the use of chemicals or that use eco-friendly chemical agents. This review examines recent progress in understanding the effect of thinning methods on the physiology, tree growth and fruit quality of cherries, discusses horticultural practices aimed to ensure regular cropping and their influence on fruit quality, and provides suggestions for future research.

Meningioma (MGM) is the most common type of intracranial tumor in adults. The validation of novel prognostic biomarkers to better inform tumor stratification and clinical prognosis is urgently needed. Many molecular and cellular alterations have been described in MGM tumors over the past few years, providing a rational basis for the identification of biomarkers and therapeutic targets. The role of receptor tyrosine kinase (RTKs), including those of the ErbB family of receptors, as oncogenes has been well established in several cancer types. Here, we review histological, molecular, and clinical evidence suggesting that RTKs, including the epidermal growth factor receptor (EGFR, ErbB 1), as well as other members of the ErbB family, may be useful as biomarkers in MGM.