Associating the most influential parameters with the product distribution is of uttermost importance in complex catalytic processes such as fluid catalytic cracking (FCC). These correlations can lead to the information-driven catalyst screening, kinetic modeling and reactor design. In this work, a dataset of 104 uncorrelated experiments, with 64 variables, has been obtained in an FCC simulator using 6 types of feedstock (vacuum gasoil, polyethylene pyrolysis waxes, scrap tire pyrolysis oil, dissolved polyethylene and blends of the previous), 36 possible sets of conditions (varying contact time, temperature and catalyst/oil ratio) and 3 industrial catalysts. Principal component analysis (PCA) has been applied over the dataset, showing that the main components are associated with feed composition (27.41% variance); operational conditions (19.09%) and catalyst properties (12.72%). The variables of each component have been correlated with the indexes and yields of the products: conversion, octane number, aromatics, olefins (propylene) or coke, among others.

In this work, we report the magnetocaloric effect (MCE) in a quantum dot corresponding to an electron interacting with an antidot, under the effect of an Aharonov-Bohm flux subjected to a parabolic confinement potential. We use the Bogachek and Landman model, which additionally allows the study of quantum dots with Fock-Darwin energy levels for vanishing antidot radius and flux. We find that the Aharonov-Bohm flux (AB-flux) strongly controls the oscillatory behaviour of the MCE, thus acting as a control parameter for the cooling or heating of the magnetocaloric effect. We propose a way to detect AB-flux by measuring temperature differences.

Any quantization maps linearly functions on a phase space to symmetric operators in a Hilbert space. Covariant integral quantization combines operator-valued measure with symmetry group of the phase space. Covariant means that the quantization map intertwines classical (geometric operation) and quantum (unitary transformations) symmetries. Integral means that we use all ressources of integral calculus, in order to implement the method when we apply it to singular functions, or distributions, for which the integral calculus is an essential ingredient. In this paper we emphasize the deep connection between Fourier transform and covariant integral quantization when the Weyl-Heisenberg and affine groups are involved. We show with our generalisations of the Wigner-Weyl transform that many properties of the Weyl integral quantization, commonly viewed as optimal, are actually shared by a large family of integral quantizations.

Although the signaling function of Na/K ATPase is been studied for decades, the chasm between the pumping function and the signaling function of Na/K – ATPase is still an open issue. This article explores the relationship between ion pumping and signaling with attention to the amplification of oxidants through this signaling function. Starting with some experimental observations published by our laboratories and others, we develop some predictions regarding cellular oxidant stress.

Reliable evapotranspiration (ET) estimation is a key factor for water resources planning, attaining sustainable water resources use, irrigation water management, and water regulation. During the past few decades, researchers have developed a variety of remote sensing techniques to estimate ET. The Earth Engine Evapotranspiration Flux (EEFlux) application uses Landsat imagery archives on the Google Earth Engine platform to calculate the daily evapotranspiration at the local field scale (30 m). Automatically calibrated for each Landsat image, the EEFlux application design is based on the widely vetted Mapping Evapotranspiration at high Resolution with Internalized Calibration (METRIC) model and produces ET estimation maps for any Landsat 5, 7 or 8 scene in a matter of seconds. In this research we evaluate the consistency and accuracy of EEFlux products that are produced when standard US and global assets are used. Processed METRIC products for 58 scenes distributed around the western and central United States were used as the baseline for comparison. The goal of this paper is to compare the results from EEFlux with the standard METRIC applications to illustrate the utility of the EEFlux products as they currently stand. Given that EEFlux is derived from METRIC, differences are expected to occur due to differing calibration methods (automatic versus manual) and differing input datasets. The products compared include the fraction of reference ET (ETrF), actual ET (ETa), and surface energy balance components net radiation (Rn), ground heat flux (G), and sensible heat flux (H), as well as Ts, albedo and NDVI. The product comparisons show that the intermediate products of Ts, Albedo, and NDVI, and also Rn have similar values and behavior for both EEFlux and METRIC. Larger differences were found for H and G. Despite the more significant differences in H and G, results show that EEFlux is able to calculate ETrF and ETa values comparable to the values from trained expert METRIC users for agricultural areas. For non-agricultural areas such as semi-arid rangeland and forests, the automated EEFlux calibration algorithm needs to be improved in order to be able to reproduce ETrF and ETa that is similar to the manually calibrated METRIC products.

Animal-focused research has been crucial for scientific advancement however, in this matter, rodents are still taking a starring role. Coming out from merely being supportive of evidence found in rodents, the use of fish models has slowly taken a more central role and expanded its overall contributions in areas such as social sciences, evolution, physiology, and recently in translational medical research. In neurosciences, zebrafish has been widely adopted, contributing to our understanding of the genetic control of brain processes, and the effects of pharmacological manipulations. However, discussion continues regarding the paradox of function versus structure, when fish and mammals are compared, and on the potentially evolutionarily conserved nature of behaviour across fish species. From the behavioural stand point we explored aversive/stress and social behaviour in selected fish models, and refer to the extensive contributions of stress and monoaminergic systems. We suggest that, in spite of marked neuroanatomical differences between fish and mammals, stress and sociality are conserved at the behavioural and molecular levels. We also suggest that stress and sociality are mediated by monoamines in predictable and non-trivial ways, and that monoamines could “bridge” the relationship between stress and social behaviour. To reconcile the level of divergence with the level of similarity, we need neuroanatomical, pharmacological, behavioural, and ecological studies conducted in the laboratory and in nature. These areas need to add to each other to enhance our understanding of fish behaviour and ultimately how this all may translate to better model systems for translational studies.

Sea level rise and increased storm events, urge cities to develop governance capacity. However, a cohesive conceptual and empirical-based understanding of what governance capacity implies, how to measure it, and what cities can learn, is largely lacking. Understanding the influence of context is critical to address this issue. Accordingly, we aim to identify crosscutting contextual factors and their influence in impeding, enhancing or prioritising different elements of governance capacity to address urban flood risk. By assessing governance capacity through nine conditions and 27 indicators in two Dutch and two cities in the UK, three crosscutting contextual factors are identified: 1) flood probability and impact, 2) national imposed institutional setting, and 3) level of authority to secure long-term financial support. We found that contextual factors explain differences in urban capacity-priorities within and between both countries. The institutional setting in the UK and recent political devolution emphasized the role of citizen awareness, stakeholder engagement, entrepreneurial agents, and the overall necessity for local capacity-development. The Dutch focus on flood safety through centralised public coordination reduces flood probability but also inhibits incentives to reduce flood impacts and reduced public awareness. In conclusion, the three identified contextual factors enable a better understanding of capacity-building priorities.

Interviews from strength and conditioning coaches across all levels of athletic competition identified their two biggest concerns with the current state of wearable technology: (a) the lack of solutions that accurately capture data "from the ground up" and (b) the lack of trust due to inconsistent measurements. The purpose of this research is to investigate the use of liquid metal sensors, specifically Liquid Wire sensors, as a potential solution for accurately capturing ankle complex movements such as plantar flexion, dorsiflexion, inversion, and eversion. Sensor stretch linearity was validated using a Micro-Ohm Meter and a Wheatstone bridge circuit. Sensors made from different substrates were also tested and discovered to be linear at multiple temperatures. An ankle complex model and computing unit for measuring resistance values were developed to determine sensor output based on simulated plantar flexion movement. The sensors were found to have a significant relationship between the positional change and the resistance values for plantar flexion movement. The results of the study ultimately confirm the researchers' hypothesis that liquid metal sensors, and Liquid Wire sensors specifically, can serve as a mitigating substitute for inertial measurement unit (IMU) based solutions that attempt to capture specific joint angles and movements.

Nearly 50 years ago, Francis Crick propounded the frozen accident scenario for the evolution of the genetic code along with the hypothesis that the early translation system consisted primarily of RNA. Under the frozen accident perspective, the code is universal among modern life forms because any change in codon assignment would be highly deleterious. The frozen accident can be considered the default theory of code evolution because it does not imply any specific interactions between amino acids and the cognate codons or anticodons, or any particular properties of the code. The subsequent 49 years of code studies have elucidated notable features of the standard code, such as high robustness to errors, but failed to develop a compelling explanation for codon assignments. In particular, stereochemical affinity between amino acids and the cognate codons or anticodons does not seem to account for the origin and evolution of the code. Here I expand Crick’s hypothesis on RNA-only translation system by presenting evidence that this early translation already attained high fidelity that allowed protein evolution. I outline an experimentally testable scenario for the evolution of the code that combines a distinct version of the stereochemical hypothesis, in which amino acids are recognized via unique sites in the tertiary structure of proto-tRNAs, rather than by anticodons, expansion of the code via proto-tRNA duplication and the frozen accident.

This study evaluated the impact of rough, smooth, and very smooth Titanium (Ti) and zirconia (Zr) surfaces on the neutrophil expression of elastase (NE), matrix metalloproteinase (MMP)-8, MMP-9 and extracellular traps (NETs). Ti and Zr disks with a diameter of 10mm and a thickness of 1.5mm were processed using diamond-impregnated polishing burs and paste to generate rough (R; Ra= > 3µm), smooth (S; Ra= ≥1 to 1.5µm) and very smooth (VS; Ra= <0.1µm) surfaces. Peripheral neutrophils from Wistar rats were cultured on these surfaces and the culture supernatants subsequently analyzed for the presence of NE, MMP-8, and MMP-9 by ELISA, while NETs formation was demonstrated immunohistochemically by staining neutrophils with CD16b and DNA with DAPI. Overall, the expression of NE and MMP-8 were significantly higher from neutrophils cultured on both on Ti and Zr rough surfaces compared to the very smooth surface (R>S>VS) after 2h and 4h of culture. The expression of MMP-9 also increased with culture time however no significant surface effects on expression were observed. Similarly, rough Ti and Zr surfaces (R & S) also showed significantly larger NETs formation compared to the very smooth surface (VS) after 4h and 8h culture. Our findings suggest that increasing surface roughness on Ti and Zr triggers higher secretion of NE, MMP-8 and NETs formation.

In the context of carbon emissions peak, environmental issues highlight the importance of the green economy, how does economic agglomeration release growth potential and enable the coordinated development of the economy and environment? There are few works of literature to analyze it within the framework of spatial economy. This paper constructs a theoretical model to clarify the influence mechanism of economic agglomeration on green total factor productivity (GTFP), and then uses a dynamic SDM model to test the theoretical hypothesis. This contribution has three main findings. First, there is a "U-shaped" curve relationship between economic agglomeration and GTFP, and the formation mechanism is that economic agglomeration has a threshold effect on the agglomeration externalities such as infrastructure sharing, knowledge spillover, and labor market upgrading. Second, the mismatch of industrial structure is an important reason that the economic agglomeration in this region has not produced an obvious spatial spillover effect on other regions; Relaxing restrictions on the concentration of economic activity to regional centers would contribute to the improvement of GTFP. Third, GTFP has the classic "snowball effect" in the time dimension, but has the obvious "warning effect" in the space and time dimension. Based on this, this paper believes that at the present stage, it is necessary to adapt to the layout of economic geography, promote the rational allocation of human resources in the territorial space, promote the coordination between economic agglomeration and the development goal of green economy, and at the same time, it is necessary to cultivate the cooperative linkage mechanism of green economy development and transformation among cities.

Aedes (Stegomyia) albopictus is a mosquito native to Southeast Asia. Currently, it has a wide distribution in America where natural infection with arboviruses of medical and veterinary importance has been reported. In spite of their importance in the transmission of endemic arbovirus, basic information of parameters affecting their vectorial capacity is poorly investigated. The aim of the work was to update the distribution range of Ae. albopictus in the Americas, review the blood-feeding patterns and compare the minimum infection rate (MIR) of the dengue virus (DENV) between studies of vertical and horizontal transmission. The current distribution of Ae. albopictus encompasses 21 countries in the Americas. Extensive review has been conducted for the blood-feeding patterns of Ae. albopictus. The results suggest that the mosquito is capable of feeding on 16 species of mammals and five species of avian. Humans, dogs, and rats are the most common host. Eight arboviruses with the potential to infect humans and animals have been isolated in Ae. albopictus. In the United States of America (USA), Eastern equine encephalitis virus, Keystone virus, La Crosse Virus, West Nile virus, and Cache Valley virus were isolated in the Asian mosquito. In Brazil, Mexico, Colombia, and Costa Rica, DENV (all serotypes) has been frequently identified in field-caught Ae. albopictus. Overall, the estimated MIR in Ae. albopictus infected with DENV is similar between horizontal (10.95) and vertical transmission (8.28). However, in vertical transmission, there is a difference in the MIR values if the DENV is identified from larvae or adults (males and females emerged from a collection of eggs or larvae). MIR estimated from larvae is 14.04 and in adults is 4.04. In conclusion, it has to be highlighted that Ae. albopictus is an invasive mosquito with wide phenotypic plasticity to adapt to broad and new areas, it is highly efficient to transmit the DENV horizontally and vertically, it can participate in the inter-endemic transmission of the dengue disease, and it can spread zoonotic arboviruses across forest and urban settings.

The aim of the work was to update the distribution range of Aedes (Stegomyia) albopictus Skuse in the Americas, review the blood-feeding patterns and compare the minimum infection rate (MIR) of the dengue virus (DENV) between studies of vertical and horizontal transmission. The current dis-tribution of Ae. albopictus encompasses 21 countries in the Americas. Extensive review has been conducted for the blood-feeding patterns of Ae. albopictus. The results suggest that the mosquito is capable of feeding on 16 species of mammals and five species of avian. Humans, dogs, and rats are the most common host. Eight arboviruses with the potential to infect humans and animals have been isolated in Ae. albopictus. In the United States of America (USA), Eastern equine encephalitis virus, Keystone virus, La Crosse Virus, West Nile virus, and Cache Valley virus were isolated in the Asian mosquito. In Brazil, Mexico, Colombia, and Costa Rica, DENV (all serotypes) has been frequently identified in field-caught Ae. albopictus. Overall, the estimated MIR in Ae. albopictus infected with DENV is similar between horizontal (10.95) and vertical transmission (8.28). However, in vertical transmission, there is a difference in the MIR values if the DENV is identified from larvae or adults (males and females emerged from a collection of eggs or larvae). MIR es-timated from larvae is 14.04 and in adults is 4.04. In conclusion, it has to be highlighted that Ae. albopictus is an invasive mosquito with wide phenotypic plasticity to adapt to broad and new areas, it is highly efficient to transmit the DENV horizontally and vertically, it can participate in the inter-endemic transmission of the dengue disease, and it can spread zoonotic arboviruses across forest and urban settings.

The energy, momentum and the action associated with the time domain transition radiation fields are investigated. The results show that for a charged particle moving with speed $v$, the longitudinal momentum associated with the transition radiation is approximately equal to $E/c$ for values of $1-v/c$ smaller than about 10^-3 where $E$ is the total radiated energy and c is the speed of light in free space. The action of the transition radiation, defined as the product of the energy dissipated and the duration of the emission, increases as $1-v/c$ decreases and, for an electron, it becomes equal to $h/4\pi$ when $v=c-v_m$ where $v_m$ is the speed associated with the lowest energy state of a particle confined inside the universe and h is the Plank constant. Combining these results with Heisenberg’s uncertainty principle, an expression for the electronic charge based on other fundamental physical constants is derived. The best agreement between the experimentally observed electronic charge and the theoretical prediction is obtained when one assumes that the actual size of the universe is about 250 times larger than the visible universe.

Pufferfish (Tetraodontidae) inhabiting the Mediterranean Sea can represent an emerging public health risk due to the possible accumulation of marine neurotoxins (TTXs and STXs) in their tissues. In this study, the presence of pufferfish species in the Strait of the Sicily (Lampedusa Island, Italy) was investigated using a Citizen Science (CS) approach involving local fishermen. A sub-group of specimens collected were sent to the National reference Laboratory on Marine Biotoxins for de-termining the presence of TTXs using a validated HILIC-MS/MS method on fish tissue. The STXs presence was also screened in some specimens. Overall, 56 specimens, identified as Sphoeroides pachygaster (Müller &Troschel, 1848) were collected. Data on specimen total length, body weight, fishing method, catch area (with relative depth temperature and salinity) were analyzed and compared with the S. pachygaster records reported in Guardone et al. (2018), which were appro-priately updated until 2022. All the analysed tissues were found to be negative for both TTXs and STXs. The key role of the CS in monitoring potentially toxic marine species was proved. Outcomes from this study, the first investigating the toxicity of S. pachygaster in Italian waters, may provide useful data for the proper assessment of this emerging risk

The investigation of membrane proteins, key constituents of cells, is hampered by the difficulty and complexity of their in vitro synthesis, of unpredictable yield. Cell-free synthesis is herein employed to unravel the impact of the expression construct on gene transcription and translation, without the complex regulatory mechanisms of cellular systems. Through the systematic design of plasmids in the immediacy of the start of the target gene, it was possible to identify translation initiation and the conformation of mRNA as the main factors governing the cell-free expression efficiency of the human voltage dependent anion channel (VDAC), a relevant membrane protein in drug-based therapy. A simple translation initiation model was developed to quantitatively assess the expression potential for the designed constructs. A scoring function is proposed that quantifies the feasibility of formation of the translation initiation complex through the ribosome-mRNA hybridization energy and the accessibility of the mRNA segment binding to the ribosome. The scoring function enables to optimize plasmid sequences and semi-quantitatively predict protein expression efficiencies. This scoring function is publicly available as webservice XenoExpressO at rna.tbi.univie.ac.at.

Exposure to air pollution have detrimental effects on the elderly, women, people with pre-existing medical conditions, people living in poverty and children. The aim of the study was to investigate the extent of community awareness and knowledge on the health risks associated with exposure to air pollution. A cross-sectional study design was used for the study, using self-administered questionnaires. A simple random sampling technique was used to select 376 respondents. Systematic sampling method was applied to select the households. SPSS version 26 was used to analyze data. Of 376 respondents, 221 were males and 154 females. 113 were aged between 23-47years and 353 were aware that if they don’t protect themselves against polluted air, they may get sick, with age and educational status associated with their awareness (p &lt; 0.05). About 361 knew what air pollution is and 188 think the air they breathe in Annadale is moderate. A total number of 278 said they feel sick/uncomfortable when the quality of air is bad. About 293 knew that people are exposed to air pollution by breathing contaminated air and 237 identified sewage smell as the main cause of air pollution. Respondents who identified internet and television as the main source of information were 199 and 177 respectively. Those who were not aware of actions implemented to reduce air pollution were 180. Based on the results of the study, respondents are aware of the dangers of exposure to polluted air, and it is necessary that air pollution risk communication strategies be implemented to empower residents.

Since the college years represent an important period for forming oral health behaviors during adulthood, it is important that college students establish proper oral health-promoting behaviors. Therefore, this study aims to identify the effects of oral health education experience, knowledge, and practice behaviors on oral health care self-efficacy among college students, as studies related to this topic are scarce. A total of 236 college students participated in a questionnaire survey comprising 21 questions. The collected data were analyzed through a chi-square test, independent-samples t-test, and logistic regression analysis using SPSS version 22.0. Participants with oral health education experience showed higher levels of oral health knowledge and practice behaviors than those who did not, and the odds of them having oral health care self-efficacy was 3.743 higher. Moreover, a one-unit increase in oral health knowledge and practice behaviors was associated with a 1.199 and 1.351 increase in oral health care self-efficacy, respectively. For oral health promotion among college students, oral health care self-efficacy reinforcement programs tailored to college students should be developed. These should focus on expanding oral health education opportunities to promote improvement in oral health care self-efficacy by strengthening oral health knowledge and the motivation to practice oral health behaviors.

Background Recently, a surge of COVID 19 was observed globally, regionally and nationally. With increasing numbers of cases, the frequency of long COVID is on the rise. Management and control of long COVID depend on changes in respect of human behaviors and requires an understanding of knowledge, attitudes, and practices (KAP) regarding health threats. MethodsA descriptive cross sectional study using online survey to gather data on the socio-economic background, knowledge, attitudes and practices on long-term complications of COVID. Results: Out of 201 respondents, 89.2% participants have heard about long-term complications of COVID 19. Only 35.9% have demonstrated adequate knowledge in the questions relating to co-morbidities and risk factors of COVID-19. A total of 92.2% believe that they should adhere to preventive measures following vaccination. Less than 60 % were following the advice on avoiding unnecessary travel and crowded places. Further, less than 50% were following COVID preventive measures. ConclusionAlthough the majority of participants have heard about long-term complications and common symptoms, the knowledge regarding co-morbidities that can lead to severe disease and long COVID was not satisfactory. The attitudes of the participants indicated increasing concern about long COVID. Practices indicate lack of adherence to key measures such as avoiding crowded places. These findings highlight the need for further increasing of awareness.

In this paper, we investigate p-adic q-integral (q-Volkenborn integral) on ℤ_{p} of p-adic gamma function via their Mahler expansions. We also derived two q-Volkenborn integrals of p-adic gamma function in terms of q-Daehee polynomials and numbers and q-Daehee polynomials and numbers of the second kind. Moreover, we discover q-Volkenborn integral of the derivative of p-adic gamma function. We acquire the relationship between the p-adic gamma function and Stirling numbers of the first kind. We finally develop a novel and interesting representation for the p-adic Euler constant by means of the q-Daehee polynomials and numbers.

The main goal of the present paper is to construct some families of the Carlitz’s q-Bernoulli polynomials and numbers. We firstly introduce the modified Carlitz’s q-Bernoulli polynomials and numbers with weight (α, β) and investigate their some explicit properties and identities arising from the bosonic q-Volkenborn integral on ℤ_p. We then define the modified degenerate Carlitz’s q-Bernoulli polynomials and numbers with weight (α, β) and obtain some recurrence relations and other identities. Moreover, we derive some correlations with the modified Carlitz’s q-Bernoulli polynomials with weight (α, β), the modified degenerate Carlitz’s q-Bernoulli polynomials with weight (α, β), the Stirling numbers of the first kind and second kind.

Alcohol use disorder (AUD) is a chronic and relapsing biopsychosocial condition that leads to a wide range of medical, psychological, social, economic, and personal issues. The study investigated the predictors of quality of life (QoL) among male patients seeking treatment for AUD based on attention, cognitive instability, motor impulsiveness, perseverance, and self-control dimensions of trait impulsivity. The study also investigated the potential mediating effect of anxiety and alcohol use severity in this relationship. After an initial assessment, participants underwent comprehensive evaluations, including sociodemographic data, clinical history, and QoL domains. Evaluations also covered AUD severity, impulsivity dimensions, cognitive functions, craving intensity, depression, anxiety and self-efficacy. Multiple linear regressions were employed to identify associations between independent variables and QoL. Attention dimension of impulsivity and anxiety symptoms significantly predicted physical health QoL. Self-control dimension and severity of alcohol use disorder predicted psychological QoL. Indirect effect of attention dimension on physical health was significant [effect =-1.082,95% CI (-2.008, -0.3598)].The significance of impulsivity in influencing QoL for individuals with AUD has profound clinical implications. These findings underscore the importance of addressing anxiety and impulsivity in managing AUD, given their considerable impact, particularly on QoL, ultimately shaping treatment outcomes.

Background: The progression of periodontal diseases at national Portuguese level and its public awareness are of great interest, mainly due to the high burden of periodontitis. Objectives: To evaluate the prevalence progression of periodontal diseases in Portugal and correspondent public awareness, between 2004 and 2017, by using data from the Global Burden of Disease (GBD), Directorate-General of Health (DGH) and Google® Trends (GT). Methods: For the period 2004-2017, Portuguese national data of periodontal diseases prevalence were searched in the Institute for Health Metrics and Evaluation of GBD and DGH and for public awareness, GT comparison tool between Portuguese words for “Periodontitis”, “Gingivitis”, “Gums” and “Periodontal disease” trends was used. Results: For the period 2004-2017, the overall prevalence of periodontitis slightly increased from 11.3% to 11.7%. During that period the GT search term “Gums” (“Gengivas”) was the most relevant. It increased steadily over time while the search term “Periodontal disease” (“Doença periodontal”) decreased, being these search trends significantly correlated (

The following article presents an analysis of the impact of the Environmental, Social and Governance-ESG determinants on Hospital Emigration to Another Region-HEAR in the Italian regions in the period 2004-2021. The data are analysed using Panel Data with Random Effects, Panel Data with Fixed Effects, Pooled Ordinary Least Squares-OLS, Weighted Least Squares-WLS, and Dynamic Panel at 1 Stage. Results show that HEAR is negatively associated to E, positively to S and negatively associated to the G within the ESG model. The data were subjected to clustering with a k-Means algorithm optimized with the Silhouette coefficient. The optimal clustering with k=2 is compared to the sub-optimal cluster with k=3. The results suggest a negative relationship between the resident population and hospital emigration at regional level. Finally, a prediction is proposed with machine learning algorithms classified based on statistical performance. The results show that the Artificial Neural Network-ANN algorithm is the best predictor. The ANN predictions are critically analyzed in light of health economic policy directions.

Childhood glaucoma encompasses congenital and juvenile primary glaucoma, which are heterogeneous, uncommon, and irreversible optic neuropathies leading to visual impairment with poorly understood genetic basis. Our goal was to identify gene variants associated with these glaucoma types by assessing the mutational burden in 76 matrix metalloproteinase-related genes. We studied 101 childhood glaucoma patients with no identified monogenic alterations using next-generation sequencing. Gene expression was assessed through immunohistochemistry. Functional analysis of selected gene variants was conducted in cultured cells and in zebrafish. Patients presented a higher proportion of rare variants in four metalloproteinase-related genes, including CPAMD8 and ADAMTSL4, compared to controls. ADAMTSL4 protein expression was observed in the anterior segment of both the adult human and zebrafish larvae's eye, including tissues associated with glaucoma. In HEK-293T cells, expression of four ADAMTSL4 variants identified in this study showed that two variants (p.Arg774Trp and p.Arg98Trp) accumulated intracellularly, inducing endoplasmic reticulum stress. Additionally, overexpressing these ADAMTSL4 variants in zebrafish embryos confirmed partial loss-of-function effects for p.Ser719Leu and p.Arg1083His. Double heterozygous functional suppression of adamtsl4 and cpamd8 zebrafish orthologs resulted in reduced volume of both the anterior eye chamber and lens within the chamber, supporting a genetic interaction between these genes. Our findings suggest that accumulation of partial functional defects in matrix metalloproteinases-related genes may contribute to increased susceptibility to early-onset glaucoma and provide further evidence supporting the notion of a complex genetic inheritance pattern underlying the disease.

Shrimp is the most important aquaculture industry. Therefore, we determined the effect of nitrifying-probiotic bacteria on water quality, growth, survival, immune response and intestinal microbiota of Litopenaeus vannamei cultured without water exchange. In vitro, only B. licheniformis used TAN, nitrites and nitrates since nitrogen bubbles were produced. TAN decreased significantly in the treatments with bacilli and LAB, but no differences were observed in nitrites. Nitrates were significantly higher in the treatments with bacteria. The final weight and survival of shrimp in the bacterial treatments increased significantly and superoxide anion increased significantly only in LAB treatment. The activity of phenoloxidase decreased significantly in the treatment with LAB and with the mixture of bacilli and LAB. Shrimp treated with bacilli in the water showed lower species richness. The gut bacterial community after treatments was significantly different from that of the control. Linoleic acid metabolism was positively correlated with final weight and superoxide anion, whereas quorum sensing was correlated with survival. Thus, bacilli and LAB in the water of hyperintensive culture systems act as nitrifers, modulate the intestinal microbiota and immune response, and improve the growth and survival of shrimp. This is the first report on Pediococcus pentosaceus and Leuconostoc mesenteroides as nitrifying bacteria.

The International Roughness Index (IRI) is the standard scale for evaluating road roughness in many countries in the world. The Taipei City government actively promotes a Road Smoothing Project and plans to complete the rehabilitation of the main and minor roads within its jurisdiction. This study aims to detect the road surface roughness in Taipei City and recommend appropriate IRI thresholds for road rehabilitation. A total of 171 asphalt concrete pavement sections in Taipei City with a total length of 803.49 km were analyzed and compared by IRI. The longitudinal profile of the detected road sections was measured using an inertial profiler. The statistical analysis showed that the IRI value prior to road leveling was mainly distributed between 5 and 8 m/km, while the IRI value after road leveling was mainly distributed between 3 and 4.5 m/km. This confirms that the implementation of the Road Smoothing Project has a significant effect on improving road smoothness. Moreover, based on the analysis results, it is recommended that the IRI threshold value for road rehabilitation in Taipei City be set at 4.50 m/km.

Salvia ceratophylloides (Ard.) is an endemic, rare, threatened plant species recently rediscovered in very few individuals in two different sites of South Italy. The study of within-plant variation more than among-plant one is fundamental to understand the plant adaptation to the local conditions, especially in rare species, and consequently to preserve plant biodiversity. Here, we reported the variation of the morpho-ecophysiological and metabolic traits between the sessile and petiolate leaf of S. ceratophylloides plants in two different sites for understanding the adaptation strategies for surviving in these habitats. The S. ceratophylloides individuals exhibited different net photosynthetic rate, maximum quantum yield, light intensity for the saturation of the photosynthetic machinery, stomatal conductance, transpiration rate, leaf area, fractal dimension and some VOCs between the different leaf types. This within-plant morpho-physiological and metabolic variation was depended on the site. These results provide empirical evidence of sharply within-plant variation of the morpho-physiological traits and VOCs profiles in S. ceratophylloides which could be because of adaptation to the local conditions.

Clathrate hydrate-based technologies have been considered as promising and sustainable alternatives for the effective management of the climate change risks related to emissions of carbon dioxide, produced by human activities. This work presents a combined experimental and computational investigation of the effects of the operational procedures and characteristics of the experimental configuration, on the phase diagrams of CO2-H2O systems and CO2 hydrates formation, growth and dissociation conditions. The operational modes involved (i) the incremental (step-wise) temperature cycling and (ii) the continuous temperature cycling processes, in the framework of an isochoric pressure search method. Also, two different high pressure PVT configurations were used, of which one encompassed a stirred tank reactor and the other incorporated an autoclave of constant volume with magnetic agitation. The experimental results implied a dependence of the subcooling, (P, T) conditions for hydrate formation and dissociation and thermal stability of the hydrate phase on the applied temperature cycling mode, and the technical features of the utilized PVT configuration. The experimental findings were complemented by a thermodynamic simulation model and other calculation approaches, with the aim to resolve the phase diagrams including the CO2 dissolution over the entire range of the applied (P, T) conditions.

The main apple production areas of the Loess Plateau, China limited information on soil and leaf K, Ca, Mg, and micronutrients and their relationships in these orchards. Therefore, we collected soil and leaf samples from the main apple production areas of the Loess Plateau and analyzed them to assess soil and leaf K, Ca, Mg, Cu, Mn, Zn, and Fe as well as to visualize their relationships. The investigated apple orchards in the Loess Plateau exhibited low soil Mn, moderate soil K, Zn, Fe, and high Cu and deficiency of leaf Fe, Moderate of leaf K, Cu, and Zn, high of leaf Ca, Mg, and excessive in leaf Mn. Moreover, soil pH negatively correlated with soil K, Cu, Zn, and Fe and positively related to exchangeable Mg, leaf Mg, and leaf Ca. Exchangeable Ca was negatively related to soil Mg, Cu, Mn, and Fe. Meanwhile, exchangeable K also negative correlation with leaf Mg, indicating the importance of soil pH and exchangeable Ca as an indication of apple produc-tion. In addition, available Zn also negative correlation with leaf Ca and leaf Zn. Soil micronu-trient contents in Shaanxi province trend lower than in other provinces, suggesting that soil Mn and Zn deficiency may be a limiting factor for apple production. Consequently, it is suggested that the soil pH be reduced in the Loess Plateau, replenishing soil organic matter and micronutrients, controlling soil Ca and Mg, and the Loess Plateau must pay attention to controlling K fertilizer application to avoid soil exchangeable cation and enhancing organic fertilizer application to improve soil micronutrients (Zn, Mn, and Fe) are highly necessary and it is necessary to enhance leaf Fe.

Abstract: In the Western of Mekong Delta belong to Vietnam a amount of alluvium sediments flows from upstream China to Vietnam, they are going from the river branches to in the Mekong Delta, then get out the Western Sea of Vietnam. This sediments accumulated gradually elevation the new land. The increase elevation of the land creates a fertile coastal plain where mangrove forests with a rich ecosystem of plants and animals. Over time, these forests change, with different plant species succeeding each other. This aims of this study to finding plant species in the forest, classification of forest types based on ecological regions, assessement the biodiversity of tree species, and compare the abundance communities, measuring the growth of the forest in these regions. In 2023, a comprehensive survey was conducted by using a systematic approach. The number of survey plots have done depend on the density of the forest, Base on the width of the forest range, the number of survey plots from 500m to 1,000m set up 1-2 plots, sub region set up from 10 to 15 plots. In total, 68 plots have done established in the erea, the area of plot is 100 square meters (10m x 10m). Using the statistical software in forestry to survey and analysis data. The result provided a complete picture of the tree species composition, distribution, and community structure characteristics in each ecological sub-region. The result of survey showed in the sub-region 1 have seven species as Sonneracia ovate, Rhzophora apiculata, Excoecaria agallochha, Avicennia officinalis, Avicennia alba, Agricerasvcoculatum, Bruguiera gymnorhiza. In the sub region 2 have eleven species as Sonneracia caseolaris, Annona reticulata, Lumnitzera littorea, Lumnizera racemosa, Rhizophora apiculata, Excoecaria agallocha, Avicennia officinalis, Avicennia alba, Hibiscus tillacerus, Bruguiera gymorhiza, Aericeras comiculatum. In the sub region 3 have eight as Sonratia caseolaris, Rhizophora mucronata, Rhizophora apiculata, Excoecaria agallocha, Avicennia marine, Avicennia alba, Bruguiera gymorhiza. In the region 4 have ten species as Sonneracia caseolaris, Sonneracia ovate, Lumnizera racemosa, Rhizophoa apiculata, Excoecaria agallocha, Avicennia marina, Avicennia officinalis, Aviecnnia alba, Bruguiera gymorhiza, Bruguiera cylindrica The total species of the mangrove forest in the Western Mekong Delta have 16 species from 11 plant families have been identified. Among these species have 6 dominant species include Avicennia oficinali),Avicennia alba, Rhizophora apiculata, Excoecaria agallocha, Someratia caseolaris, and Bruguiera yipamoriza. Two species easy grow on the new land are Avicennia officinalis Avicennia alba this findings show they can develope on the original new land for the shore of the Western Mekong Delta. The surveyed results also calculated the average of the height, diameter (D1.3), canopy, health of the nature mangrove tree for each sub region and total region. From these results showed the division of foresty structure, the structure of height, diameter (D1.3), canopy, vitality of the sub region and total region in the Western Mekong Delta.

The purpose of this work was to achieve non-destructive detection of the internal defects of in-shell walnuts using X-ray radiography technology based on improved Faster R-CNN network model. First, the FPN structure was added to the feature extraction layer to extract richer image information. Then ROI Align was used to instead of ROI Pooling for eliminating the localization bias problem caused by quantization operation. Finally, the Softer-NMS module was introduced to the final regression layer with the predicted bounding box for improving the localization accuracy of the candidate boxes. The results indicated that the internal defects in intact walnuts with shells could be identified effectively using the proposed improved network model in this study. Specifically, the discrimination accuracy of the in-shell sound, shriveled and empty-shell walnuts were 96.14%, 91.72% and 94.80% respectively, and the highest overall accuracy can reach 94.22 %. Contrasted with original Faster R-CNN network model, the mAP and F1-value of the improved Faster R-CNN model increased by 5.86% and 5.65%, respectively. Consequently, the proposed method can be applied for the in-shell walnuts with shriveled and empty-shell defects.

21-hydroxylase deficiency (21OHD), the most common form of congenital adrenal hyperplasia (CAH), is associated with pathogenic variants in CYP21A2 gene. The clinical form of the disease ranges from classic or severe to non-classic (NC) or mild late onset. The CYP21A2 gene is located on the long arm of chromosome 6, within the RCCX region, one of the most complex loci in the human genome. The 3’untranslated sequence of CYP21A2 exon 10 overlap the last exon of TNXB gene (these genes lie on the opposite strands of DNA and have the opposite transcriptional direction) that encodes an extracellular matrix glycoprotein tenascin-X (TNX). A recombination event between TNXB and its pseudogene TNXA causes a 30-kb deletion producing a chimeric TNXA/TNXB gene (CAH-X chimera) where both CYP21A2 and TNXB genes are impaired. This genetic condition characterizes a subset of patients with 21OHD who display the hypermobility phenotype of Ehlers Danlos Syndrome (hEDS) (CAH-X Syndrome). The aim of this study was to assess the prevalence of CAH-X syndrome in an Italian cohort of patients with 21OHD. At this purpose, 196 probands were recruited. Multiplex ligation-dependent probe amplification (MLPA) and Sanger sequencing were used to identify the CAH-X genotype. Twenty-one individuals showed the heterozygous continuous deletion involving the CYP21A2 and part of the TNXB gene. EDS-related clinical manifestations were identified in most patients carrying the CAH-X chimera. A CAH-X prevalence of 10.7% was estimated in our population.

Contrary to previous belief, mutation frequency also depends on how essential a gene is. Mutation frequency is substantially lower in the coding regions of essential genes and higher in less essential genes. Even though the mutation bias based on gene essentiality is incapable of invalidating Darwinism, this bias certainly added a rather unsubtle obstacle to it by contradicting a paradigm that was widely held: that mutations are random in the sense of being independent of their function for the organism. The reason this bias occurs is currently unknown. Here, I present the theoretical solution of why genetic mutations also depend on whether the gene is more or less essential. My theory is that each gene or epigene has its own level of resistance to entropic elevation, something directly proportional to the frequency of its expression. The more a gene is expressed, the higher is its resistance to entropic elevation and vice versa. For genetic information to be conserved instead of suffering mutation, a relevant factor is also the frequency of its expression. The energy expenditure directed to a gene of current utility engenders resistance to entropic elevation of the information encoded in that gene, as energy enables not only the existence of information but also its conservation. My theory—which is based on fundamental physics and contains predictions that can be empirically tested—solves a crucial problem in evolutionary biology and genetics; if I am right, I will have established a biological law. Finally, my aim here is also to provide a substantial advance in knowledge about the relationship between information, genetics, and thermodynamics.

Digital infrastructure is the basic guarantee for digital transformation, it is of practical significance to discuss how to effectively use digital infrastructure construction to promote the digital transformation of tourism enterprises. This paper examines the impact of digital infrastructure construction on tourism enterprises based on the "Broadband China" policy as a quasi-natural experiment. A-share tourism listed enterprises from 2010 to 2021 are studied using the double difference method. Findings indicate that digital infrastructure construction positively and significantly influences the digital transformation of tourism enterprises, driven by digital leadership and innovation capabilities. State-owned, small-scale, and high-growth tourism enterprises benefit more from this transformation. The results suggested that the state should develop differentiated digital infrastructure plans based on the specific conditions of different tourism enterprises. Additionally, tourism enterprises should enhance digital leadership and innovation capabilities through strategic changes and digital applications to amplify the impact of digital infrastructure on their transformation.

Osteoclasts (OCs) are the multinucleated, bone-resorbing giant cells originally differentiated from a monocyte/macrophage lineage. OC differentiation and maturation, also called osteoclastogenesis, are strictly regulated by a variety of signaling pathways primarily induced by the interactions of two prerequisite cytokines, macrophage colony-stimulating factor (M-CSF) and the receptor activator of nuclear factor-κB ligand (RANKL) to their respective surface receptors, c-fms and RANK, in OC precursors (pre-OCs). Rab11 has emerged as the spatiotemporal regulators of intracellular vesicular transport in the endosomal recycling system; however, how it regulates osteoclastogenesis is incompletely understood. OC-triggered bone resorption is best characterized to be immensely dependent upon lysosomal function, the intracellular acidic organelles containing more than 50 acid hydrolases secreted into bone matrix microenvironment (BME). On the contrary, it is little known about the lysosomal function on modulating the turnover of c-fms and RANK surface receptors via Rab GTPase-mediated vesicular transport, thereby dictating osteoclastogenesis. In this review, I briefly describe the mechanism underlying lysosome-induced osteoclastogenesis via the Rab11-mediated modulation of the surface receptors in OCs.

The analysis reported here is unique in that it is the first study of the original data from the Pfizer/BioNTech BNT162b2 mRNA vaccine clinical trial (CA4591001) to be carried out by a group unaffiliated with the trial sponsor. Our study is a forensic analysis of the 38 trial subjects who died between July 27, 2020, the start of Phase 2/3 of the clinical trial, and March 13, 2021, the data end date of their 6-Month Interim Report. Phase 2/3 of the trial involved 44,060 subjects who were equally distributed into two groups and received Dose 1 of either the BNT162b2 mRNA vaccinated or the Placebo control (0.9% normal saline). At Week 20, when the BNT162b2 mRNA vaccine received Emergency Use Authorization from the U.S. FDA, subjects in the placebo arm were given the option to be BNT162b2 vaccinated. All but a few accepted. Surprisingly, a comparison of the number of subject deaths per week during the 33 Weeks of this study found no significant difference between the number of deaths in the vaccinated versus placebo arms for the first 20 weeks of the trial, the placebo-controlled portion of the trial. After Week 20, as subjects in the Placebo were unblinded and vaccinated, deaths among this still unvaccinated cohort of this group slowed and eventually plateaued. Deaths in the BNT162b2 vaccinated subjects continued at the same rate. Our analysis revealed inconsistencies between the subject data listed in the 6-Month Interim Report and publications authored by Pfizer/BioNTech trial site administrators. Most importantly, we found evidence of an over 3.7-fold increase in number of deaths due to cardiovascular events in BNT162b2 vaccinated subjects compared to Placebo controls. This significant adverse event signal was not reported by Pfizer/BioNTech. Potential sources of these data inconsistencies are identified.

In this paper, possibilities for the bandwidth and wavelength utilization in future next-generation passive optical networks are presented and reasons for realizing and utilizing of extended dynamic wavelength and bandwidth algorithms for NG-PON2 networks are analyzed. Next, principles of the effective dynamic bandwidth allocation are introduced in details, focused on the importance of the decision criterion optimization. For achieving better bandwidth utilization of dedicated wavelengths, this paper is focused on the novel effective dynamic bandwidth allocation algorithm with adaptive allocation of wavelengths to ONU units and on the optimization of the decision criterion. For analyzing the novel extended DWBA algorithm utilizing for various wavelength allocation cases possible in NG-PON2 networks, a simulation program with the enhancement of the EDBA algorithm is realized. Finally, an optimization of the decision criterion defining a minimum bandwidth utilization of the actual wavelength is executed for NG-PON2 networks based on the hybrid TWDM technique.

The main purpose of study is to identify the impact of COVID-19 pandemic on the green financing of banks and non-bank financial institutions (NBFIs) in an emerging economy such as Bangladesh. Also, this study shows the green banking activities of the banks and NBFIs during the pandemic. To analyze the impact of the pandemic on green financing, secondary data were obtained from the quarterly and annual reports of Bangladesh Bank (BB) on green financing as well as the annual reports and websites of 61 banks and 34 NBFIs in Bangladesh for the period 2021–2019. Subsequently, the study deployed dependent t-test statistics, growth rate (year-on-year), descriptive statistics, relative percentage changes, and varying tables and graphs to analyze the obtained secondary data. The empirical findings revealed that during the COVID-19 pandemic, there was an increase in green finance for all banks and NBFIs compared to before the epidemic, indicating that the pandemic had no negative impact on the total green finance growth of all banks and NBFIs. On the other hand, compared to the pre-pandemic period, bank-wise growth in green financing was higher for state-owned commercial banks (SOCBs), specialized banks (SDBs), and private commercial banks (PCBs) but lower for foreign-owned commercial banks (FCBs) during the COVID-19 epidemic. This suggests that the pandemic does not affect the expansion of green finance by SOCBs, SDBs and PCBs but significantly impacted the growth of green financing by FCBs. Furthermore, the research findings showed that the total outstanding and classified loans within the green finance investment decrease for both banks and NBFIs during the COVID-19 pandemic. The results indicated that the Bangladeshi banks’ level of automation towards green banking were satisfactory during the pandemic. Therefore, major policy implications for the green economic recovery by the government, BB, and managers of the banks and financial institutions in emerging economies like Bangladesh were discussed.

We report that ethanol, used together with water, plays a crucial role in tuning the structures of a zirconium-based Metal-Organic Framework, the 12-connected MOF-801, and the possible mechanisms of this modulating effect. By employing the cosolvent system of ethanol and water just under room temperature without the presence of a monotopic carboxylic acid as the modulator, MOF-801 in various morphologies of different sizes can be synthesized. The linear correlation between the ethanol/water ratio and the crystal sizes is also demonstrated. The growth mechanism is mainly explained by ethanol’s binding with the metal ion clusters and the Marangoni Flow Effect. Ethanol competes with the linker molecules in coordinating with the Zr metal clusters, a role similar to that of the modulators. The Marangoni Flow Effect, which dominates at a certain solvent ratio, further promotes the 1-D alignment of the MOF-801 crystals.

Marburg virus (MARV) is a lipid-enveloped negative sense single stranded RNA virus, which can cause a deadly hemorrhagic fever. MARV encodes seven proteins, including VP40 (mVP40), a matrix protein that interacts with the cytoplasmic leaflet of the host cell plasma membrane. VP40 traffics to the plasma membrane inner leaflet, where it assembles to facilitate the budding of viral particles. VP40 is a multifunctional protein that interacts with several host proteins and lipids to complete the viral replication cycle, but many of these host-interactions remain unknown or are poorly characterized. In this study, we investigated the role of a hydrophobic loop region in the carboxy-terminal domain (CTD) of mVP40 that shares sequence similarity with the CTD of Ebola virus VP40 (eVP40). These conserved hydrophobic residues in eVP40 have been previously shown to be critical to plasma membrane localization and membrane insertion. An array of cellular experiments and confirmatory in vitro work strongly suggests proper orientation and hydrophobic residues (Phe281, Leu283, and Phe286) in the mVP40 CTD are critical to plasma membrane localization. In line with the different functions proposed for eVP40 and mVP40 CTD hydrophobic residues, molecular dynamics simulations demonstrate large flexibility of residues in the EBOV CTD whereas conserved mVP40 hydrophobic residues are more restricted in their flexibility. This study sheds further light on important amino acids and structural features in mVP40 required for its plasma membrane localization as well as differences in the functional role of CTD amino acids in eVP40 and mVP40.

In the present study, a mathematical model of non-steady partial differential equation from the process of oxygen mass transport in the human pulmonary circulation is proposed. Mathematical modelling of this kind of problems lead to a non-steady partial differential equation and for its numerical simulation, we have used finite differences. The aim of the process is the exact numerical analysis of the study, wherein consistency, stability and convergence is proposed. The necessity of doing the process is that, we would like to increase the order of numerical solution to a higher order scheme. An increment in the order of numerical solution makes the numerical simulation more accurate, also makes the numerical simulation being more complicated. In addition, the process of numerical analysis of the study in this order of solution needs more research work.

The eastern subterranean termite, Reticulitermes flavipes, currently inhabits previously glaciated regions of the northeastern U.S., as well as the unglaciated southern Appalachian Mountains and surrounding areas. We hypothesized that Pleistocene climatic fluctuations have influenced the distribution of R. flavipes, and thus the evolutionary history of the species. We estimated contemporary and historical geographic distributions of R. flavipes by constructing Species Distribution Models (SDM). We also inferred the evolutionary and demographic history of the species using mitochondrial (cytochrome oxidase I and II) and nuclear (endo-beta-1,4-glucanase) DNA sequence data. To do this, genetic populations were delineated using Bayesian spatial genetic clustering, competing hypotheses about population divergence were assessed using approximate Bayesian computation (ABC), and changes in population size were estimated using Bayesian skyline plots. SDMs identified areas in the north with suitable habitat during the transition from the Last Interglacial to the Last Glacial Maximum, as well as an expanding distribution from the mid-Holocene to the present. Genetic analyses identified three geographically cohesive populations, corresponding with northern, central, and southern portions of the study region. Based on ABC analyses, divergence between the Northern and Southern populations was the oldest, estimated to have occurred 64.80 thousand years ago (kya), which corresponds with the timing of available habitat in the north. The Central and Northern populations diverged in the mid-Holocene, 8.63 kya, after which the Central population continued to expand. Accordingly, phylogeographic patterns of R. flavipes in the southern Appalachians appear to have been strongly influenced by glacial-interglacial climate change.

In this investigation steel P355NH has been successfully cladded with Inconel 625 through the method of explosive welding. Explosively welded bimetal clad-plate was subjected to the two separated post weld heat treatment processes: stress relief annealing (at 620oC for 90 minutes) and normalizing (at 910oC for 30 minutes). In order to analyze the microstructure of the joint in the as-welded state and to investigate the influence of the post weld heat treatment on it, the light and scanning electron microscope observations and microhardness analysis have been performed. The examination of the diffusion zone microstructure has been performed by using the scanning transmission electron microscope. It was stated that obtained joint has characteristic wavy-shape geometry with the presence of the melted zones and severe deformed grains of both joined materials. Strain hardening of the materials in joint zone was established with microhardness analysis. In both of the heat treatments the changes in the grain structure have been observed. The normalizing heat treatment has the most significant impact on the microstructure of the joint as well as the concentration of the chemical elements in the joint zone. It was reported that due to normalizing the diffusion zone has been formed together with precipitates in the joint zone. The analysis of the diffusion zone images leads to the conclusion that the diffusion of alloying elements from Inconel 625 to steel P355NH takes place along the grain boundaries with additional formation of the voids in this area. The precipitates in Inconel 625 in the joint zone are two type of carbides – chromium-rich and molybdenum-rich. Scanning transmission electron microscope observation of the grain microstructure in the diffusion zone shows that this area consists of equiaxed grains (from the side of Inconel 625 alloy) and columnar grains (from the side of steel P355NH).

Experimental methodologies for fatigue lifetime prediction are time-intensive and susceptible to environmental variables. While the cohesive zone model is popular for predicting adhesive fatigue lifetime, entropy-based methods have also shown promise. This study aims to provide an understanding of the durability characteristics of CFRP adhesive joints by incorporating an entropy damage model within the context of the finite element method (FEM), examined the effects of different adhesive layer thicknesses on SLS models. As the adhesive layer increased in thickness, DAMAGE VARIABLES initially rose and then declined, peaking at 0.3mm. This finding provides crucial understanding into the stress behavior at the resin-CFRP interface and the resin's fatigue mechanisms.

At present there is a huge demand for experimental periodontal models to expand the knowledge in periodontal health related to Microbiome Medicine. Thus far none of the periodontal pathogens or cocktail of pathogens reported as aetiological agents of infectious chronic periodontitis to link with oral squamous cell carcinoma as the way Helicobactor pyroli linked with gastric carcinoma and other related gastric malignancies. Nevertheless, invasive pathogenic mechanisms of virulent strains of Porphyromonas gingivalis and Fusobacterium nucleatum singly as well as a duo demonstrated the progression and devastation of oral, pancreatic, and colorectal cancers in different study populations. Periodontal disease may be an inevitable consequence or complication of oral cancer patients who comprise a section of digestive tract cancers, due to suboptimal or poor oral hygiene practices due to unbearable pain and other complications of stage four oral cancer. Periodontitis is a reversible oral condition when it is treated properly at the correct stage and good oral hygiene practices and treating chronic periodontitis showed successful interventions in the prognosis of oral cancer. In the light of these scientifically proven epidemiological, laboratory, and clinical evidence this communication highlights the implication of ATP scavenging behavior and upregulation of immune regulatory receptors by intracellular Porphyromonas gingivalis based on in-vitro models on the prognosis of digestive tract cancers regarding the oral cancer patients.

We study the nonlinear absorptive and dispersive optical properties of molecular systems immersed in a thermal reservoir interacting with a Four-wave Mixing (FWM) signal. Residual spin-orbit Hamiltonians are considered in order to take into account the internal structure of the molecule. As system parameters in the dissipation processes, transverse and longitudinal relaxation times are considered for stochastic solute-solvent interaction processes. The intramolecular coupling effects on the optical responses are studied using a molecule model consisting of two coupled harmonic curves of electronic energies with displaced minima in nuclear energies and positions. In this study, it was considered the complete frequency space is considered through the pump-probe detuning, not restricting the derivations to only maximums of population oscillations. This approach opens the possibility of studying the behavior of optical responses, which is very useful in experimental design. Our results indicate the sensitivity of the optical responses to parameters of the molecular structure as well as to those derived from the photonic process of FWM signal generation.

The presented work shows that a force (electromagnetic or gravitational) field surrounding any material body is not limitless, but has the external and internal boundaries (radii). Understanding the fact of the existence of the field boundaries allows us to solve the problem of infinities that exists in electrodynamics. It is shown that there are two different electrostatic potentials: with the dimension of speed and with the dimension of the square of speed.

The feeling of emotional self-efficacy helps people understand how to handle positive and negative emotions. Emotion regulation indicates the process that helps modulate emotions, to adapt suitably to the demands of the environment. This study has a two-fold aim. Firstly, it analyses the relationships among emotion regulation; the personality traits of extraversion and emotional stability; and the feeling of efficacy of positive and negative emotions in an adolescent population. Secondly, it analyses the mediating role of personality traits (extraversion and emotional stability) in the relationship between emotion regulation and self-efficacy for positive and negative emotions. There were 703 adolescents who took part (49.9% male and 50.1% female) aged between 15 and 18 years (M=15.86, TD=0.30). The results observe the significant relationships among emotion regulation; the personality traits of extraversion and emotional stability; and self-efficacy for positive and negative emotions. The structural equation model confirms that the link between emotion regulation and self-efficacy is direct and is also mediated by the personality traits of extraversion and emotional stability. The study confirms that emotional self-efficacy beliefs are connected to the emotional regulation strategies used. Effective emotional regulation favours self-perception and emotional coping. There is a discussion about the results, which are connected to previous research.

Rapidly progressive glomerulonephritis, the term coined for a subtype of nephritic syndrome, is characterized by a state of acute loss of renal function, along with those components of renal damage on urinalysis, over a relatively short period (a few weeks or months). It is the aftermath of an acute, severe glomerular injury. RPGN, famously known as Crescentic glomerulonephritis (cGN) is not a single disease entity but a pattern that can occur in a variety of glomerular diseases caused by different pathologic lesions leading to the development of necrosis in the glomerular capillaries seen in both, systemic and kidney-restricted diseases. While still little is known about the exact etiologic cause behind the primary injury to the capillary wall leading to the development of the condition, many mechanisms are put forth to describe the pathogenesis leading to the progression. Immunopathologic studies have demonstrated that rapidly progressive glomerulonephritis can result from glomerular antibody deposition, can be immune complex-mediated, or from some as yet undefined mechanism that doesn’t involve glomerular antibody deposition. The condition is often severe with poor prognosis as it develops so rapidly and with extensive glomerular involvement, the damage is almost irreversible. Many elements can be associated with the occurrence and progression of the disease but it often involves a disruptive immune response one way or the other. RPGN’s morphological hallmark, the extensive extra-capillary cellular proliferation into the bowman’s space, commonly known as crescent formation which is often observed by electron microscopic view of the tissue biopsy, is considered one of the great diagnostic approaches for the detection of the disease. Also, the severity of the disease is related to the nature and type of immunologic process causing the disease and (in part) to the degree of crescent formation, that can only be observed by histopathological studies. This activity attempts to explain the etiology and pathogenesis involved in the development leading to the rapid progression of the disease.

While studies that examined risk perceptions in various environments have been previously conducted, the relationship between risk perceptions measured in such environments had not been examined. Such a relationship, if found, may indicate that when one learns to identify risks in one environment, they may identify risks more easily and quickly in another.This study examined the relationship between measures of risk perception in several separate environments. The findings suggest that when learning to identify and assess risks in a particular environment, one may better identify and assess risks in a similar environment – for example, learning to identify and assess risks while driving makes it easier to identify and assess risks when crossing a road. Conversely, the findings also indicate that learning to identify and assess risks in one environment does not make it possible to better identify and assess risks in a disparate environment – for example, learning to identify and assess risks while driving does not make it easier to identify and assess risks in a workplace environment. This may also suggest that while disparate environments such as the road environment and the workplace environment are perceived as two separate environments, the road environment is experienced as one single environment by both drivers and pedestrians.

The windowpane oyster Placuna placenta, lives in brackish coastal waters and has long been economically important to the Philippines because of its durable and translucent shell, which is used as a glass substitute and material for making windowpanes and handicrafts. There has been considerable degradation of the windowpane oyster fishery in past decades. Moreover, there are waste and under-exploited by-products such as the meat, which though nutritious and edible, currently has a very low value; its use being confined to dishes consumed by families in the fishing community. Historically there are instances of excellent practice in terms of regulating the windowpane oyster fishery, while in recent times there have been local initiatives to restore this and to develop high value food products from the meat. These initiatives have, however, never been followed through at a provincial or national level. Research on other molluscs and marine organisms highlights that these also contain high value pharmaceutical products. This review compiles evidence to establish the groundwork for an essential and comprehensive multidisciplinary research program centred around the windowpane oyster, which would ensure a high value for all parts of the oyster, including those currently discarded. By fostering a sustainable and circular economy within this fishery sector and its associated industry, its economic value would be amplified. This is particularly important for oyster harvesters in the Philippines, who often find themselves at the economic ladder's lower rungs. By integrating the principles of a circular economy, this initiative would not only aim to uplift the economic prospects of these harvesters but in doing so would drive the restoration of the windowpane oyster to its former range.

To achieve user retention through multifactor synergy, Internet enterprises must reduce costs and increase efficiency and sustainable development. In response to the dilemma that Internet compa-nies are experiencing increasingly high user acquisition costs and serious user churn, this paper uses a sample of 46,695 user reviews of nine product series from Xiaomi Ecological Chain. Case studies and qualitative comparative analysis are used to explore the influence mechanisms of quality of experience, brand trust, and brand attachment on users' retention intentions. Our find-ings are as follows. (1) Brand attachment alone is not necessary for high user retention intention, but user perception, cognition, and brand trust are necessary. (2) Quality of experience positively impacts brand trust, attachment, and user retention intention. Therefore, improving user perception and cognition is critical in generating high user retention intention. (3) Five configuration paths can achieve high user retention intention, three configuration paths lead to low user retention intention, and there is an asymmetric relationship between these paths. Among them, the role of quality of experience-driven configuration paths in generating user retention intention is the most prominent. (4) In the substitution relationship between configuration paths, user perception, and cognition can substitute with brand trust and attachment. Our findings have important theoretical and practical implications for revealing the realization paths of high user retention intention in Internet companies and provide a new perspective for future research.

This paper is referred to a top frontline research in life science, showing that in spite of the large variation of the physiological architecture and behavioral forms of the living systems, the fundamental structure of their informational system is basically the same, allowing the body growth/development by micro to macro-structuration processes, maintaining the same informational configuration and functions like the basic unit of life – the eukaryotic cell. Starting and based on the determination of the special/specific info-operability in living structures and the role of information in structuration and their functionality, there are discussed the informational functions of the eukaryotic/prokaryotic cells, the simplest units of life and is elaborated and presented the Informational Model of the Living Structures, showing that these structures operate on the basis of two main informational systems: one of them defined as the Programmed Informational System, composed by three components, assuring respectively the info-generation, maintenance and the proliferation/reproduction, and another one defined as the Operative Informational System, necessary for the continuous communication with the environment for adaptation, consisting in four main components referred to memory, decision, sentience and info-connection/info-selection. The revealed properties are compared with the Informational System of the Human Body, as a prototype of a living system on the top of the evolutionary scale, showing/demonstrating the compatibility of structuration and functions on the entire scale, from prokaryotic/eukaryotic unit cells to multicellular organisms – plants, animals and human, by means of a continuous adaptation process, which always follows the same line of micro to macro-structuration process based on the same Info-organization and functionality. These models are new and revolutionary in the information and life sciences

Background Obesity compromises the health of a woman's pregnancy and her newborn. Aim: To describe weight-gaining changes throughout pregnancy and evaluating outcomes for the mother and the baby. Methods A retrospective review of women who delivered singletons using Large Perinatal Registry. According to their BMI, patients were categorized into four categories: underweight, normal, overweight, and obese. Mode of delivery, gestational diabetes, hypertensive diseases, obstetric cholestasis, and postpartum hemorrhage were the main maternal outcome measures. The newborn outcome indicators were birth weight, NICU hospitalization, premature birth, and stillbirth. Results The study sample included 2,352 natives (25%) and 7,058 expatriates (75%). The majority of the sample (4726) were aged 20-29 years, 43.6% were aged 30-39 years, and 2.1% and 3.9% were aged 19 years and 40 years respectively. The incidence of hypertension, obstetric cholestasis, diabetes, postpartum hemorrhage, and cesarean section varied among the entire sample. In the underweight group, the incidence of CS was 15.7%, in the normal weight group (24.9%), in the overweight group (31.3%), and in the obese group (41%). Hypertensive disorders were 3 times more prevalent in obese women than in normal-weight women (2%), and 5.3% of obese women had PPH compared to 5. 6% of women of normal weight. The underweight groups gained the most weight during pregnancy, whereas the obese gained the least. Conclusions A significant proportion of pregnant women in our sample will become obese by the time of delivery. Obesity during pregnancy is associated with negative maternal and fetal outcomes.

The full impact of COVID-19 on policing, crime and disorder is slowly being fully unraveled. However, there remains a number of areas of policing that are yet to be examined in detail. Two of these areas include the impact on the intrinsically linked, volume of police recorded intelligence reports, and the use of stop and search. In this study we examine them symbiotically and frame them in the context of the intelligence led policing model, in particular in an effort to understand how national lockdowns in the United Kingdom affected both proactive policing approaches and the underpinning intelligence cycle. To achieve this, we use data from freedom of information requests regarding the annual levels of recorded police intelligence over a 10-year period for 20 police services. To supplement this, we examine overall national monthly volumes of stop and search activity over a 5-year period. Finally, we then use a case study approach of 3 police services to further explore changes in the conduct of stop and search such as the officer defined ethnicity, grounds for search and disposal outcomes. The findings indicate that both recorded intelligence reports and stop and search increased dramatically during periods of lockdown, despite widespread decreases in crime and social mobility. Changes in proportional impact are identified for White and Black citizens, searches for controlled drugs and the no further action disposal, but these are not consistent across police services. Potential causes and implications are then discussed and again, framed within the context of the impact on the intelligence led policing model and wider policing environment.

NMR Spectroscopy is used, in the temperature range 180-350K, to study local order and transport in liquid water (pure and confined) and its solutions with glicerol and methanol at different molar fractions. Being the liquid water thermodynamic dominated by polymorphism (two coexisting liquid phases: high- and low-density HDL and LDL) - with the LDL due to the hydrophilic HB interactions, originating in the supercooled regime the tetrahedral networking and the liquid-liquid transition – we focused our interest to hydrophobic effects (HE) on these. Nowadays, if compared to the hydrophilicity, little is known about hydrophobicity so that the main purpose of this study is to gain new information on it. We measured the relaxation times (T1 and T2) and the self-diffusion (DS). From the times we took advantage of the NMR property to follow the behaviors of each molecular component (the hydrophilic and hydrophobic groups) separately; they are studied directly and DS in terms of the Adam-Gibbs model: obtaining the configurational entropy (Sconf ) and the specific heat contributions (CP,conf ). Due to the HE all the studied quantities, behave differently. For water-glycerol the HB interaction is dominant for all the conditions, whereas for water-methanol are observable two different T-regions above and below 265 K, dominated respectively by the hydrophilicity and hydrophobicity. A situation linked to the water polymorphism. Below this temperature, where the LDL phase and the HB networking develops and grows, the times and CP,conf change behaviors leading to maxima and minima. Above it, where the HB becomes weak and less stable and the HDL dominates, the hydrophobicity determines the solution properties.

The proximal part of the colon offers opportunities to prolong the absorption window following oral administration of a drug. In this work, we used computer simulations to understand how the hydrodynamics in the proximal colon might affect the release from dosage forms designed to target the colon. For this purpose, we developed and compared three different models: a completely-filled colon, a partially-filled colon and a partially-filled colon with a gaseous phase present (gas-liquid model).The highest velocities of the liquid were found in the completely-filled model, which also shows the best mixing profile, defined by the distribution of tracking particles over time. No significant differences with regard to the mixing and velocity profiles were found between the partially-filled model and the gas-liquid model. The fastest transit time of an undissolved tablet was found in the completely-filled model. The velocities of the liquid in the gas-liquid model are slightly higher along the colon than in the partially-filled model. The filling level has an impact on the exsisting shear forces and shear rates, which are decisive factors in the development of new drugs and formulations.

A methodological similarity between Thomas Hobbes and Pëtr Kropotkin is the intention to spread a theoretical foundation to everyone, in the sense that they are willing to give to all the people a clear description of the reality and a subsequent political view. To do so, they use a scientific method, deductive (starting from empirical observations) in the case of Hobbes, inductive-deductive in the case of Kropotkin. Kropotkin underlines the educational value of the scientific method.In this work we want to highlight that, although they both start their argumentations from a materialist ontology, Hobbes and Kropotkin conjecture two completely different states of nature. Hobbes describes the state of nature through the two famous metaphors homo homini lupus (citing Plautus) and bellum omnium contra omnes, while Kropotkin introduced the theory of mutual aid. Both the theory of a state of war by Hobbes and the theory of mutual aid by Kropotkin have been revolutionary. Hobbes has been influenced by the scientific revolution initiated by Francis Bacon, one of his mentors, and Galileo Galilei, together with a criticism towards the ancient Greece philosophers, in particular Aristotle. Kropotkin has been influenced by the ground-breaking writings of Charles Darwin together with a very fruitful Russian scientific environment.We want to stress here that the disenchanted view of the human nature in Hobbes, a state of war due to the fact that everyone has rights on everything, helps him to legitimate sovereignty, while the positive view of human nature in Kropotkin, a spontaneous mutual aid among people in a community, helps him to legitimate anarchy. Therefore, the fascinating scientific methods of the two materialists Hobbes and Kropotkin to structure a solid political theory cannot neglect different views on human nature due to their historical contexts.

Biodegradable poly(ɛ-caprolactone) (PCL) and its composites or blends have gotten a lot of attention in the last decade because of their potential applications in human life and environmental remediation. As a result, there is a growing interest in the synthesis of PCL-composites/blends and their applications. Greater efforts have been made to develop biodegradable chemical materials as adsorbents that do not pollute the environment in order to replace traditional materials. Among the numerous types of degradable materials, PCL is currently the most promising, the most popular, and the best material to be developed, and it is referred to as the "green" eco-friendly material. Membranes and adsorbents for water treatment, packaging and compost bags, controlled drug carriers, biomaterials for tissues such as bone, cartilage, ligament, skeletal muscle, skin, cardiovascular and nerve tissues are just some of the applications of this biodegradable polymer (PCL). The goal of this review is to present a brief overview of PCL, its properties, syntheses of PCL, PCL composites, and PCL blends, but to provide a detailed investigation into the utility of PCL/PCL-based adsorbing agents in the removal of dyes/heavy metal ions.

The studied Khanbogd pluton of peralkaline granite is located in southern Mongolia and occupies an area of ~1000 sq. km. Previously, two ring-shaped bodies were constructed in a flattened shape pluton. However, this shape was not used for the genesis of this pluton. A recent study of Landsat image showed a several elliptical intrusions in two bodies. Chemistry interpretation shows that each intrusion differs in ASI, CIPW norms, 87Sr/86Sri and δ18O isotopes. Elliptical intrusions, uplifted country rocks between them, and roof pendants suggest magma penetration along fractured country rocks. Furthermore, two adjacent intrusive bodies and undeformed host rocks at the front part of the pluton are consistent with underplating magmatic factors during duplex-type thrusting. This magma event is also supported by low of Sr, Ba, Eu and high of Th, U, Pb anomalies and a few amount of garnet crystals in the granites, implying vapor absent melt crystallization associated with underplating. Multiple melt sources, as well as from mantle and recycled sea floor rocks, are identified by ratio of 87Sr /86Sri and δ18O values. The low isotope values, the thrust nature and 295–286 Ma ages of the alkali granites, indicate an underplate magma evidenced during Permian rifting.

Among all the active confinement techniques, the use of pre-tensioned stainless steel straps has recently gained much attention. The flexibility of the stainless steel straps allows us to bend and pass them through the thickness of the masonry, thus creating a three-dimensional strengthening system between the two opposite facings. The use of the same perforation for the passage of several straps closed in a loop generates a continuous strengthening system that prevents parts of the structure from falling and injuring the occupants during seismic events. However, the perforations can nullify the in-plane strengthening, as they act as cylindrical hinges and make the reinforcement system labile for certain strap arrangements. Diagonal compression tests on square masonry panels performed in the present study show that the straps improve neither strength nor ductility when running along the mortar head and bed joints, arranged in square meshes. Conversely, they improve both strength and ductility when the straps make angles of 45° with the mortar joints. Furthermore, the experimental results show that the straps exert an anisotropic effect that decreases the apparent in-plane Poisson ratio. They also provide new insights into the diagonal compression test and allow formulating a new proposal for the pseudo-ductility factor.

Recent evidence obtained in murine atrial cardiomyocytes suggests that the behavior of tubulin, a constituent of microtubules, is influenced by audible sounds. In the present study, the sequence and the structure of murine and human tubulins were compared with those of human TMC1 (Transmembrane channel-like protein 1), a sound-sensitive protein that is responsible for forming the pore of mechanosensory transduction channels in the hair cells of the inner ear of vertebrates, and with those of bacterial FtsZ (Filamenting temperature-sensitive mutant Z), a bacterial protein homologous to tubulin. The results show that mouse and human alpha-tubulin are 100% identical, whereas sequence homology of human TMC1 and human tubulin alpha 1A chain is scarce, with only 17.31% identity. A higher degree of similarity was observed in the presence of aromatic amino acids as well as in the propensity to form alpha-helices. Based on these results, it is hypothesized that the response of tubulin to audible sounds is mediated by the external alpha-helices as it occurs in TMC1. The sequence homology of human TMC1 and FtsZ from Lactobacillus johnsonii, is moderate with 23.40% identity, however higher than that between TMC1 and tubulin. The overall presence of aromatic amino acids in FtsZ is scarce, but helix propensity is significant. These results are discussed in the context of Orch OR, a theory that postulates that consciousness emerges from quantum computations occurring in microtubules of neurons via information processing mediated by aromatic amino acids of tubulin.

Long Covid is a heterogeneous clinical condition in which Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and brain fog stand out among the different clinical symptoms and syndromes. The cerebral metabolic alterations and neuroendocrine disorders seem to constitute an important part of Long Covid. Given the substantial lack of drugs and effective therapeutic strategies, hypothalamic phospholipid liposomes which have been on the market for several years as adjuvant therapy of cerebral metabolic alterations resulting from neuroendocrine disorders, can be taken into consideration in an overall therapeutic strategy that aims to control the Long Covid associated symptoms and syndromes. Their pharmacological mechanisms and clinical effects strongly support their usefulness in Long Covid. Our initial clinical experience corroborates this rationale. Further research is imperative in order to obtain robust clinical evidence.

Background: The global prevalence of anxiety and depressive symptoms in adolescents has increased considerably during the COVID-19 pandemic. Mental health problems may compromise glycemic control in young people with type 1 diabetes; however, evidence of improved glycemic control in adolescents with T1D appeared early during the pandemic. This qualitative study aimed to provide a more in-depth understanding of how the COVID-19 pandemic affected adolescents with type 1 diabetes routines, experiences, T1D management, behaviors, and mental health. Methods: 24 adolescents, aged 15-18 years, with DM1, joined the discussion of focus groups in the context of the summer camp for diabetes. Word frequency and thematic analysis were conducted on adolescents’ narratives. Results: The word Frequency Analysis identified 'friends', 'family', and 'home' as the most frequent terms. Seven themes were identified: (1) COVID-19 and T1D; (2) emotional reactions to the COVID-19 pandemic; (3) changes in daily life; (4) feelings of loss; (5) coping with the COVID-19 pandemic; (6) the COVID-19 pandemic as an opportunity; (7) return to (new) normality. Conclusions The COVID-19 pandemic may have represented a more stressful condition for adolescents with DM1, facing additional challenges compared to their healthy peers. The results offer directions to the diabetes care team for a customized intervention while the consequences of the pandemic on adolescents’ health continue.

Ferric uptake regulation protein is a repressor protein which binds an AT rich region of DNA (the iron box). Fur binds as a dimer in a helix turn helix mode and it is activated by iron(II) and other divalent transition metal ions at elevated concentrations in a process to regulate the ion uptake. Each transition metal ion induces certain conformational changes to aid the Fur binding, both the N-terminal and C-terminal domains take part in binding to DNA in addition to His 88 and His 86 residues. The process is discussed in view of experimental reports. Fe(II), Mn(II) and Co(II) activate Fur to bind DNA experimentally but Zinc plays a structural role and does not activate Fur to bind DNA.

As we know, the economy of science is to achieve optimal gains using scarce resources. Similarly, a problem where economies of the world are trying to resolve it is the issue of how to achieve an optimal economic contribution from limited tourism resources. According to the fact that global studies and statistics indicate that good governance is considered as the most important factor in developing countries and is considered as a strong governance of the development and as Information and Communication Technology (ICT) is effective in the interactions and economic and social exchanges of any society, it is the link between the tourism industry and the technology under the form of a robust tool and utilitarian in the tourism process called E-tourism can play a significant role. Therefore, the aim of this study was to investigate the effect of network Readiness Index and good governance on income from tourism industry. In this study, data and information about 14 selected countries were collected from Southwest Asia in the annual period of 2016-2011 in the form of panel data and the proposed model was used and estimated by the generalized torques system method. The estimated results of the model indicate that both hypotheses have a positive and significant impact on the amount of Network Readiness Index in the income of tourism industry, as well as the positive and significant effect of the index the amount of income from the tourism industry is confirmed, and their impact has been positive and significant, as well as exchange rate variables, revenue and commercial openness, also have a positive and significant effect on revenues of the tourism industry.

An increased risk of multiple sclerosis (MS) had been found when individuals had consumed large amounts of processed meat and sausages at young age (Lauer, 2014). Furthermore it was found in many studies that MS patients had acquired a number of common childhood infections at higher ages than controls. Therefore, MS patients from an epidemiological long-term investigation in Germany and different hospital controls, were evaluated for a statistical interaction of these two factors. 324 MS patients and 242 hospital controls were inquired. The study focussed on age 0 - 16. Subjects were tested for additive interaction by multiple linear regression analysis (Knol et al., 2007). There was an additive interaction of the age at any common childhood infection with the consumption of scalded sausages (regression estimate = 0.1370; standard error = 0.0603; p = 0.0239). In contrast, no such interaction could be shown for: animal fats; smoked meat (e.g. ham and bacon); and cold - smoked German salami. Thus there was a synergy of the intake of scalded sausages (e.g. frankfurters, bolognas, etc.) and age at common childhood infections, for the later risk of MS.

The interlaminar shear behavior of a [±45°] laminated carbon fiber reinforced plastic (CFRP) specimen was investigated utilizing microscale strain mapping in a wide field of view. A three-point bending device was developed under a laser scanning microscope, and the full-field strain distributions including normal, shear and principal strains of CFRP in a three-point bending test were measured using a developed sampling Moire technique. The microscale shear strain concentrations at interfaces between each two adjacent layers were successfully detected and found to be positive-negative alternately distributed before damage occurrence. The 45° layers slipped to the right relative to the -45° layers, visualized from the revised Moire phases and shear strain distributions of the angle-ply CFRP under different loads. The absolute values of the shear strain at interfaces gradually rose with the increase of the bending load, and the sudden decrease of the shear strain peak value implied the occurrence of interlaminar damage. The evolution of the shear strain concentrations is useful in the quantitative evaluation of the potential interlaminar shear failure.

Until recently, the classic approach to mineral exploration studies is to bring the field samples/drill cores collected during field studies to the laboratory followed by laborious analysis procedures to generate the analytical data. This is very expensive, time consuming and difficult for exploring vast areas. But rapid technological advances in field portable analytical instruments such as portable ultraviolet–visible and near-infrared spectrophotometers, gamma ray spectrometer, pXRF, pXRD, pLIBS, and µRaman spectrometer have changed this scenario completely and increased their on-site applications in mineral exploration studies. These instruments are currently providing direct, rapid, on-site, real-time, non-destructive, cost-effective identification, and determination of target elements, indicator minerals and pathfinder elements in rock, soil, and sediment samples. These portable analytical instruments are currently helping to obtain accurate chemical and mineralogical information directly in field with minimal or no sample preparation, and providing decision-making support during field work as well as during drilling operations in several successful mineral exploration programs. In this article, the developments in these portable devices, and their contributions in the platinum group elements (PGE), rare earth elements (REE), gold, base metals, and lithium exploration studies both on land and on ocean bed have been summarized with examples.

Using two infectious DNA clones originating from isolates obtained in Kenya, a genetic investigation of maize streak virus (MSV) pathogenicity was carried out. The susceptible Pioneer hybrid 3379 was used to investigate the virulence of kernels administered through vascular puncture inoculation. Mild [pMSV-KL (mild)] and severe [pMSV-Km(severe)] parental infectious MSV DNA clones were linked with considerable changes in the severity of chlorotic streaks and stunting of seedlings. Using restriction endonuclease fragments, chimeric clones were created from parental clones in order to investigate the genetic elements influencing these changes. Restrictions mapping was used to validate clone identities. For genomic portions encoding the replication initiator (Rep and RepA), movement (MP), and coat (CP) protein ORFs as well as for those containing non-coding long (LIR) and short intergenic regions (SIR), putative virulence determinants were discovered. Recombinant clones with intermediate symptoms included LIRs together with the first 189 nt of the Rep/RepA ORFs from the pMSV-Km (Severe) and pMSV-KL (Mild) ORFs. Symptoms identical to those of parental clones were produced by complementary substitution of the Rep/RepA ORF. When compared to pMSV-Km (Severe), the recombinant clone having MP, CP, and SIR genome sequences from pMSV-KL(Mild) and LIR, Rep, and RepA genome sequences from pMSV-Km (Severe) displayed much more severe symptoms and accumulated greater concentrations. The reciprocal clone, in contrast, displayed much milder signs and virus titers than pMSV-KL(Mild). The level of leaf chlorosis and plant stunting were linked with viral accumulation. These findings are consistent with the idea that MSV virulence is a polymorphic feature involving complementary sense genes (LIR and SIR) and the virion.

Owners and managers of small and medium-sized enterprises (SMEs) have changed their perspective on risk management due to the current global negative threats in the business environment. If they want to be successful and ensure the financial performance of their business, they must accept a proactive approach to reducing strategic risks in connection with operational risks. The aim of the article is to identify and quantify the differences in operational risk management and its effect on the financial management of SMEs between four Central European countries. The statistical sample of SMEs consisted of 1,090 owners and top managers. Data collection was carried out through a questionnaire in the period December 2022-January 2023. Statistical hypotheses were evaluated with the application of correlation analysis and linear regression modelling. Empirical findings have confirmed that operational risk management has a significant positive impact on improving the profitability of the enterprise; on reducing the enterprise's indebtedness as well as in the context of reducing the enterprise's inability to pay its obligations. However, there are disparities in the perception of this influence between owners/managers with regard to the country in which they carry out their business activities. The findings are important for both national and multinational organizations and entities dealing with risk management in the business environment of small and medium-sized enterprises.

PAHs contaminants have toxic, carcinogenic, and even mutagenic properties. Screening bacteria from different sources capable of carrying out the biodegradation of PAHs is important for mapping and mobilization purposes and applying them to polluted hydrocarbon environments. The study aimed to compare the biodegradation power of two types of bacteria isolated from different sources against PAHs. The method applied is the interaction between bacterial suspen-sion and pyrene contaminated waste for 30 days. Biodegradation products in organic compounds were analyzed using GC/MS and FTIR. The analysis results found several indications of the performance of bacterial biodegradation, namely: the aggressiveness of biodegradation of Bl bacteria against pyrene was relatively more dominant than Sb bacteria. The percentage of to-tal bacterial biodegradation for product type Sb was (39.00 %), and that of the product of bacteri-al degradation type Bl (was 38.29 %). The biodegradation products of the test bacteria (Bl and Sb) were relatively similar to pyrene, in the form of alcohol and carboxylic acid organic com-pounds. It was concluded that there was no significant difference in biodegradation per-formance between Bl and Sb bacteria on for pyrene. Both types of bacterial isolates from differ-ent sources can carry out the function of biodegradation of pyrene.

The article deals with the current state of energy consumption and CO2 emissions in the urban area of building clusters. There are many requirements, such as those set by the EU (FIT 55, decarbonisation in 2050, full electromobility in 2035, or mandatory annual reductions in energy consumption and CO2 production) or societal requirements, such as stable energy supply and its optimisation while significantly reducing CO2 emissions. This inspired us to design an energy model of a building (EMB) with smart grid implementation in a decentralized sustainable energy system. Simulation model studies were carried out to show the possibility of their application in the process of fully satisfying energy needs in terms of reducing their environmental impact. A decentralized photovoltaic system (microgrid) connected to a distribution grid. The main objective is to propose an original methodology for the design of smart "Nearly Zero Energy Buildings" (NZEB) and a subsequent solution for energy sustainability. This has led us to use HOMER, PV*SOL, Monte Carlo and DesignBuilder software which were chosen from the range of options that were and are available. Subsequently, a synthesis of the selected software solutions was carried out and a new model - the "Energy Model of a Building" (EMB) - was proposed in the process of integration with the energy performance of buildings, as an original optimization basis for designing smart buildings and smart areas, and even cities. The proposed EMB has been verified and evaluated within the experiment.

Arthropod-borne viruses belonging to the flavivirus genus possess an enormous relevance in public health. Neotropical non-human primates (NPs) have been proposed to be infected more frequently with flaviviruses due to their arboreal and diurnal habits, their genetic similarity to humans and their relative closeness to humans. However, the only known flavivirus in America that is maintained by sylvatic cycles involving NPs is Yellow Fever virus (YFV), and the NPs role as potential hosts of flaviviruses is still unknown. Here, we examined flavivirus exposure in 86 free range and captive NPs of Costa Rica to evaluate their involvement in flavivirus transmission cycles and their potential as flavivirus hosts. We used a highly-specific micro plaque reduction neutralization test (micro-PRNT) to determine the presence of antibodies against YFV, Dengue virus 1-4 (DENV), Zika virus, West Nile Virus (WNV) and Saint Louis Encephalitis virus (SLEV). We found evidence of seropositive NPs to DENV-1 8.2% (homotypic –3/86, heterotypic – 4/86), SLEV 15.1% (homotypic – 10/86, heterotypic – 2/86), WNV 2.3% (homotypic – 2/86) and 8.1% (7/86) undetermined Flavivirus species. No antibodies against YFV or ZIKV were found. This work provides compelling serological evidence of exposure in NPs of flaviviruses associated with urban cycles, i.e. DENV, and confirms decades of circulation of SLEV in the same environments. Also, the range of years of sampling and the socioeconomic region was statistically significant for the presence of Dengue and Flavivirus undetermined seropositive individuals, respectively. Both the years and socioeconomic regions with greater seroprevalence coincide with the years and socioeconomic regions with high numbers of Dengue human cases for the country. Our work suggests bidirectional? circulation of different flaviviruses between humans and wildlife with public health importance and underscores the necessity of further surveillance for flaviviruses in the humans/wildlife interface in Central America.

This study explores innovation and sustainability within the Construction, Environment, and Territory (CAT) curriculum in Italian Upper Secondary Schools. It begins with an overview of Education for Sustainable Development (ESD) and its integration at various educational levels, focusing on technical/vocational curricula in Upper Secondary Education. The study examines the evolution of the technician's role, the CAT pathway in the Italian educational system, and comparisons with other European contexts. It analyzes the alignment between skills developed during the CAT course and the professional competencies required by the community and job market. A survey conducted among recent graduates and companies involved in the Pathways for Transversal Skills and Orientation (PCTO) at a CAT-focused school reveals significant training and professional needs related to Sustainable Development. To address these needs, the study proposes integrating ministerial programs in professional disciplines such as Topography, Building and Technological Systems Design, and Geopedology, Economics, and Appraisal, with new modules dedicated to Sustainable Development. These modules are to be integrated into the last three years of the course, utilizing the autonomy and flexibility in curricular planning per-mitted by current regulations. Additionally, the proposal includes actions to redistribute existing educational modules within ministerial programs without increasing teaching hours. An initial step in updating professional discipline programs involves implementing a cross-disciplinary educational path within Civic Education, focusing on Sustainable Mobility. The investigation, evaluation, and educational design process presented in this study aims to serve as a model for swiftly implementing structured curriculum reforms, emphasizing educational innovation and comprehensive student preparation to meet emerging social and professional needs.

Objective: Taiwan Government’s organizations have endeavored to promote the applications of big data and open data. The “My Health Bank” is one of the measures promoted by the National Health Administration, Ministry of Health and Welfare. This study proposes the perspective of the “value co-creation” with the attempt to extend the concept of service ecosystem and apply it on the platform of My Health Bank to examine whether people (patients, families, and caregivers) can promote their health literacy? Method: This cross-sectional study, with people that have registered at “My Health Bank” as subjects. Complying with the inclusion criteria, 401 questionnaires were delivered, with 391 valid ones, excluding those incompletely and inaccurately filled. Result: That the affecting factors of the co-creation of values: age, education level, annual income, and platform operation show to be significant ( p＜0.05); and gender, occupation, and resource exchange do not reach the significant level (p＞0.1). Conclusion: We found My Health Bank changed the inertia of “value creation” in the traditional medical value, it allows the traditional medical and healthcare industry to expose to the impacts of the mega trend of the internet, the transformation of the platform in a necessary trend.

The Chiltan Formation is a potential hydrocarbon-producing reservoir in the Indus Basin, Pakistan. However, its diagenetic alterations and heterogeneous behavior lead to significant challenges in accurately characterizing the reservoir and production performance. This manuscript aims to utilize Chiltan Limestone core samples to conduct a detailed analysis of the diagenetic impacts on reservoir quality. The formation evaluation was carried out through thin section analysis, SEM-EDS and FTIR investigation, as well as plug porosity and permeability measurements under different stress conditions. In results, Petrography revealed three microfacies with distinct diagenetic features and micro-nano fossil assemblages, including intraclasts, pelloids, bioclasts, and stylolites. Diagenesis has a significant impact on petrophysical properties, leading to increased reservoir heterogeneity. The specified depositional environment exposed the alteration of the Chiltan formation during distinct diagenetic phases in marine, meteoric, and burial settings. Pore morphology and mineralogy reveals complex micro-pore structure with various carbonate mineral phases and cement types in the formation samples. The average porosity and permeability of the core samples were found to be 3.4% and 0.449 mD respectively, indicating poor reservoir quality. Stress sensitivity was determined using Klinkenberg corrected permeability, which indicated a reduction in measured and absolute permeability due to increasing confining stress, further reducing the pore throat structure of the formation. In conclusion, the Chiltan formation possesses intricate reservoir heterogeneity and varied micro-pore structure caused by diagenesis and depositional settings. The formation is classified as a low-quality reservoir due to its non-uniform pore geometry, which exhibits low petrophysical properties resulting from overburden stress. The results of this study provide solid foundation in reservoir characterization and quality assessment which has implications for exploring and exploitation of indigenous resources.

A substantial body of phenomenological and theoretical work over the last few years strengthens the possibility that the vacuum energy density (VED) of the universe is dynamical, and in particular that it adopts the `running vacuum model' (RVM) form, in which the VED evolves mildly as $\delta \rho_{\rm vac}(H)\sim \nu_{\rm eff} m_{\rm Pl}^2{\cal O}\left(H^2\right)$, where $H$ is the Hubble rate and $\nu_{\rm eff}$ is a (small) free parameter. This dynamical scenario is grounded on recent studies of quantum field theory (QFT) in curved spacetime and also on string theory. It turns out that what we call the `cosmological constant', $\Lambda$, is no longer a rigid parameter but the nearly sustained value of $8\pi G(H)\rho_{\rm vac}(H)$ around (any) given epoch $H(t)$, where $G(H)$ is the gravitational coupling, which can also be very mildly running (logarithmically). Of particular interest is the possibility suggested in past works that such a running may help to cure the cosmological tensions afflicting the $\Lambda$CDM. In the current study, we reanalyze it in full and we find it becomes further buttressed. Using the modern cosmological data, namely a compilation of the latest SNIa+BAO+$H(z)$+LSS+CMB observations, we probe to which extent the RVM provides a quality fit better than the concordance $\Lambda$CDM model, paying particular emphasis on its impact on the $\sigma_8$ and $H_0$ tensions. We utilize the Einstein-Boltzmann system solver \texttt{CLASS} and the Monte Carlo sampler \texttt{MontePython} for the statistical analysis, as well as the statistical DIC criterion to compare the running vacuum against the rigid vacuum ($\nu_{\rm eff} = 0$). On fundamental grounds, $\nu_{\rm eff}$ receives contributions from all the quantized matter fields in FLRW spacetime. We show that with a tiny amount of vacuum dynamics ($|\nu_{\rm eff}|\ll 1$) the global fit can improve significantly with respect to the $\CC$CDM and the mentioned tensions may subside to inconspicuous levels.

The ratio of males to females with ASD is generally quoted as 4:1 though it is believed that there are biases preventing females being diagnosed and that the true ratio is lower. These biases have not been clearly identified or quantified. Starting with a clinical dataset of 1711 children <18 years old four different methods were employed in an inductive study to identify and quantify the biases and calculate the proportion of females missed. A mathematical model was constructed to compare the findings with current published data. The true male to female ratio appears to be 3:4. Eighty per cent of females remain undiagnosed at age 18 which has serious consequences for the mental health of young women.

Structure–function relationships in proteins have been one of the crucial scientific topics. Heme proteins have diverse and pivotal biological functions. Therefore, clarifying their structure–function correlation is significant to understand their functional mechanism and is informative for various fields of science. In this study, we constructed convolutional neural network models for predicting protein functions from the tertiary structures of heme-binding sites (active sites) of heme proteins to examine the structure–function correlation. As a result, we succeeded in the classification of oxygen-binding protein (OB), oxidoreductase (OR), proteins with both functions (OB–OR), and electron transport protein (ET) with high accuracy. Although the misclassification rate for OR and ET was high, the rates between OB and ET and between OB and OR were almost zero, indicating that the prediction model works well between protein groups with very different functions. However, predicting the function of proteins modified with amino acid mutation(s) remains a challenge. Our findings indicate a structure–function correlation in the active site of heme proteins. This study is expected to be applied to the prediction of more detailed protein functions such as catalytic reactions.

The biological function of rapid eye movement (or REM) sleep—one of the great mysteries of neuroscience—remains unknown. Here, I demonstrate that the biological function of REM sleep is to heighten brain alertness, significantly reducing the high vulnerability of deep sleep. Every organism with a nervous system must undergo deep sleep: a necessity that comes with substantial vulnerability. Deep sleep, by lowering alertness, compromises the organism's safety, putting its survival at risk. Therefore, REM sleep is a necessary adaptation for any organism that must sleep. My primary goal here is to present a comprehensive conceptual framework—supported by extensive empirical evidence—to connect numerous disparate empirical findings under a unified theory of the biological function of REM sleep. Additionally, I also provide a historical narrative to explain its origin and subsequent evolution. The theory I developed to explain the function of REM sleep is not only widely corroborated but has also resisted numerous attempts at refutation. This allows me to claim that I solved this great enigma of neuroscience.

Interoperability has become crucial in the world of electronic health records (EHR), allowing for seamless data exchange and integration across diverse settings. It facilitates the integration of disparate systems, ensures that patient records are accessible, and enhances the care delivery process. The current interoperability landscape for electronic health records is saddled with challenges hindering efficient interoperability. Existing interoperability frameworks for EHR have failed to properly address many of these challenges relating to data exchange, security and privacy. To address these challenges, the TASSIPS framework is proposed as a comprehensive approach to achieving efficient interoperability. TASSIPS integrates robust security and privacy measures, providing real-time access to electronic health records that enable precise diagnoses, timely treatment plans and improved patient outcomes. TASSIPS offers a holistic and effective solution to healthcare interoperability challenges. An evaluation of TASSIPS via comparison with exicting frameworks concluded that it is an advancement in framework design for interoperabil-ity of EHR.

This study describes the relationship between the epikarst and the surface karst of climatic karsts. Bedrock resistivity values along VES measurements were measured and calculated. The elevation difference between the surface and the karstwater table and the annual distribution of precipitation were calculated in certain karst areas. The resistivity of the epikarst can be high (drawdown dolines) and low (subsidence dolines). In the former case, the cavity index and the vertical drainage of the epikarst are heterogeneous, while in the latter they are homogeneous. Considering the above characteristics, two types of doline development are possible. A feature development controlled by the epikarst (tajga karst, soil-covered karst of the temperate belt, glaciokarst), by the epikarst and the karstwater (polje karst, fengcong karst), and controlled predominantly by karstwater (intermountain plain of fenglin karst, tropical karren). At feature development controlled by the epikarst, drawdown dolines (soil-covered karst) and subsidence dolines (covered karst) develop, in the case of feature development controlled by the epikarst and karstwater, drawdown dolines, subsidence dolines and feature assemblages with subsidence dolines and katavotra (polje karst, fengcong karst) are formed. At feature development controlled predominantly by karstwater, subsidence dolines (intermountain plain of fenglin karst), and giant grikes (tropical karren) develop.

The rapid and accurate detection of parasites is crucial for timely curative intervention in parasitosis and for epidemiological surveillance. To meet the needs of clinical diagnosis, it is imperative to develop a diagnostic tool based on nucleic acid that combines the sensitivity and specificity of established nucleic acid amplifica-tion tests with the speed, cost-effectiveness, and convenience of isothermal amplification methods. A new nu-cleic acid detection method, utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nuclease, holds promise for point-of-care testing. Specifically, the CRISPR-Cas12a system has demonstrated numerous advantages for detecting parasites, with hopeful outcomes for identify-ing malaria, toxoplasmosis, and other parasitic ailments. This review provides an overview of how CRISPR-Cas12a can be utilized for parasite detection, evaluates its advantages and disadvantages, and sug-gests ways to improve the efficiency and sensitivity of CRISPR-Cas12a-based assays.

While most published research on COVID-19 focused in a few countries and especially during the second wave of the pandemic and the vaccination period, we turn to the first wave (march-May 2020) to examine sentiment and emotions expressed by Twitter users in Greece. We use computational methods combining opinion mining and the application of a modified emotion lexicon with Social Network Analysis to explore the subtleties behind an overarching negative sentiment. Our analysis has shown that popular sentiment isn’t stable nor monolithic, since underlying emotions may be a better predictor of attitudes during a crisis.

In this review we collect some recent achievements in the accurate and efficient solution of the Nonlinear Schrödinger Equation (NLSE), with the preservation of its Hamiltonian structure. This is achieved by using the energy-conserving Runge-Kutta methods named Hamiltonian Boundary Value Methods (HBVMs) after a proper space semi-discretization. The main facts about HBVMs, along with their application for solving the given problem, are here recalled and explained in detail. In particular, their use as spectral methods in time, which allows efficiently solving the problems with spectral space-time accuracy.

Public transport is one of the most important functions of a city, which can have a major impact on the elements that support sustainable development: society, the environment and the economy. In order for residents and tourists of the Petrosani Basin to benefit from a predictable, systematized public transport system with well-established travel times, it is necessary to analyze some current factors and trends regarding public transport in the Petrosani Basin. This prospective study confirms that the use of the public transport system in the Petrosani Basin is decreasing, a decrease mainly associated with the lack of spatial accessibility, comfort and safety of passengers (inappropriate stations, lack of air conditioning in means of transport, lack of a modern fare system, the lack of means of information in the stations, etc.) but also with the change in people's attitude imposed by the protective measures taken as a result of the coronavirus pandemic. However, it should be noted that the public transport system in the Petrosani Basin is strongly influenced by the geographical characteristics of the Petrosani Basin, by the sharp decrease in the population (negative natural increase; reduction of the school population) but also by the increase in the number of private cars.

Gout is at least 3 times more prevalent in men than in women. However, concurrent with rising total gout prevalence, complex factors, including comorbidities, diet and lifestyle, and aging, have promoted higher gout prevalence in females. This narrative review focuses on summarizing recent developments in the landscape of gout in women, and mechanisms involved. New knowledge on sex hormone effects on both urate-excreting and urate-reabsorbing transporters, and higher hypertension and chronic kidney disease prevalence in women compared to men, may help explain why gout incidence rises robustly after menopause in women to approach that in men. Racial and ethnic factors, risk profiles based on heritable genetic polymorphisms of urate transporters, and diet, body mass index, and lifestyle factors differ according to sex. In addition, sex differences in clinical phenotypes and outcomes of gout and non-gout illnesses include more frequent comorbidities, more pain and disability during gout flare, different perceptions of disease burden, and more frequent severe cutaneous hypersensitivity reaction to allopurinol in women. Significantly, women with gout also have poorer COVID-19 outcomes than do men. Collectively, such findings support the potential clinical benefits of tailoring gout and hyperuricemia treatment according to sex.

Infectious diseases are one of the leading causes of death among children under the age of 5 with 60% of those deaths preventable with prophylaxis. Vaccinations prove to be the most succesfull and effective public health tool of infectious dieases preventions. Poland first implemented a program of mandatory childhood vaccinations in 1960. The program is updated each year and provides parents with a clear schedule to follow. The program includes vaccinations against 12 diseases (tuberculosis, hepatitis B, rotaviral disease, diphtheria, pertussis, tetanus, Haemophilus influenzae, Streptococcus pneumoniae, poliomyelitis, measles, mumps and rubella) mandatory for all children and funded by the government. It also includes recommended vaccinations which costs are covered by a patient. Immunization rates are monitored nationwide and reported to the Chief Sanitary Inspector. With recent outbreaks of infectious diseases among young children the obligation lies on the government to provide childhood immunizations, and largely on the physicians to provide reliable education about infectious diseases in children and safety of vaccinations to the parents.

The Individual Work Performance Questionnaire (IWPQ) can be defined as the individual be-haviour capable of generating value and competitive advantage for the organization. It is a mul-ti-dimensional construct, consisting of task performance, contextual performance and counter-productive work behavior. Albeit important, a comprehensive measure of individual job per-formance is still lacking in the Italian context. This paper describes the validation of an Italian adaptation of the IWPQ. A back translation procedure was used. To investigate the psychometric properties of the 18-item Italian IWPQ, 1053 participants (502 men, 47.7%; 551 women, 52.3%) were enrolled; the age of participants ranged between 19 and 69 (Mage=34.6, SD= 11.8), and for the oc-cupation, the sample is classified in blue collar 32.5%, pink collar 33% and white collar 34.5%. Exploratory and confirmatory factor analyses and multigroup confirmatory factor analyses were performed to test the structural factor of the IWPQ. The results supported the goodness of the IWPQ in the Italian context and the scales were found consistent with the original structure. One item was omitted due to a low factor loading, resulting in a final 17-item structure. Multigroup confirmatory factor analysis showed that the factor solution was invariant across both gender and occupational category and found evidence of metric invariance, uniqueness and scalar invariance, and structural invariance. Convergent and discriminant validity were also tested and showed.

There are compelling evidences that the immune system is to confer protection not only against foreign pathogens but also against cancer. But if the immune system can’t detect every malignant cell, and at the same time a suitable tumor microenvironment (TME) is formed by inflammatory processes, a carcinogenesis is not longer to be blocked. Once the tumor cells have found their niche, the tumor cells are trained by immune cells turned inside out in how to deal with the immune system "out there". The TME is a training camp and protected space for tumor cells. The Corona crisis has revived a therapeutic approach that was thought to be almost dead: RNA vaccines. Despite decades of research and many clinical studies, no registered RNA cancer vaccine exists today. But the SARS-CoV-2 RNA vaccines against COVID-19 disease have demonstrated their dangerous weaknesses. RNA vaccines manipulate the innate immunity and make the body susceptible to any viral infection. There is also the possibility of integration into the genome. The few approved therapeutic cancer vaccines show little effect. Despite some successes, immunotherapies remain ineffective for most patients with cancer. Powerful therapeutic cancer vaccines remain an unfulfilled dream, a misconception.

Serotonergic neurons constitute one of the main systems of neuromodulators, whose diffuse projections regulate the functions of the cerebral cortex. Serotonin (5-HT) is known to play a crucial role in the differential modulation of cortical activity related to behavioral contexts. Certain aspects of the 5-HT signaling framework hint at its potential role as a modulator of activity-dependent synaptic changes within the critical period of the primary visual cortex (V1). Cells of the serotonergic system are among the first neurons to differentiate and operate. During postnatal development, ramifications from raphe nuclei become massively distributed in the visual cortical area, remarkably increasing the availability of 5-HT for the regulation of excitatory and inhibitory synaptic activity. A substantial amount of evidence has demonstrated that synaptic plasticity at pyramidal neurons of the superficial layers of V1 critically depends on a fine regulation of the balance between excitation and inhibition (E/I). Therefore, 5-HT could play an important role in controlling this balance, providing the appropriate excitability conditions that favor synaptic modifications. In order to explore this possibility, the present work used in vitro intracellular electrophysiological recording techniques to study the effects of 5-HT on the E/I balance of V1 layer 2/3 neurons, during the critical period. Serotonergic modulation of the E/I balance has been analyzed on spontaneous activity, evoked synaptic responses, and long-term depression (LTD). Our results pointed out that the predominant action of 5-HT implies a reduction of the E/I balance. 5-HT promoted LTD at excitatory synapses while blocking LTD at inhibitory synaptic sites, thus shifting the Hebbian alterations of synaptic strength towards lower levels of E/I balance.

The paper proposes an approach to determine the optimal number of medical specialists in the region. According to the author's theoretical model, in order to maximize public welfare, the marginal contribution of the last physician recruited to the growth of the public utility function should be equal to the marginal cost of attracting him and providing conditions for his work. To empirically assess the contribution of physicians to the number of lives saved, the CVD mortality rate is modeled using the instrumental variable method. On average, a 1% increase in the number of cardiologists is associated with a 0.6% decrease in cardiovascular mortality. At the level of provision with cardiologists in the amount of 1 per 100 thousand people, their marginal contribution to the number of lives saved is not less than 129 per 100 thousand people, with a further decrease by 5 people with the increase in the level of provision by 1 unit. The use of the obtained results will increase the validity of managerial decisions and in determining the optimal level of doctors when choosing between alternative possibilities of spending money on hiring doctors of different profiles or other expenses, especially in the case of limited resources.

By using the Black-Derman-Toy (BDT) model, we predict the future trend of the riskless rate, and then we build an equation that relates the market price of zero-coupon bonds and the theoretical price of zero-coupon bonds calculated using a binomial option pricing model. Based on this, we can find the implied daily return μ, implied natural upturn probability p, and implied daily volatility σ with respect to different time-to-maturity values of zero-coupon bonds. With these results, we can give some suggestions to bond traders.

Rheocasting is getting more and more attention from the electronics and automotive industries. Mechanical properties and thermophysical properties such as thermal and electrical conductivity are affected by the amount of solid phase generated in the slurry process. The RheoMetal process specifically has a significant deviation from the expected solid fraction. This deviation is discussed, explained and a way to control the excess generation of solid phase is proposed.

As a matter of course, the first gene must be formed in the absence of gene. On the other hand, many biopolymers including gene are produced under the genetic system in extant organisms. Thus far, no idea explaining how the first gene was formed in the absence of gene, has been proposed except the idea based on the GADV hypothesis. GADV means four amino acids; Gly [G], Ala [A], Asp [D] and Val [V]. In this article, a reliable answer to the question, how the first gene was generated, is provided. The idea is as follows. The first gene (genetic information) was formed by random joining of anticodons, GNCs, which were carried by primeval anticodon stem-loop (AntiC-SL) tRNAs produced during repeated cycles of random joining of nucleotides and degradation of oligonucleotides. However, it might be difficult for many persons to accept the idea, because tRNAs and metabolic pathways using various proteins, which are produced under genetic functions, must be used to form the first gene. Then, it is reconsidered based on definition of genetic information and minimum necessary things to generate the first gene in this article, how the first gene was generated. Consequently, it could be reconfirmed that five prototypes of members (genetic code, tRNA, metabolism, cell structure and protein) composing the fundamental life system, except gene, are certainly necessary to generate the first gene. Namely, it can be concluded that the first gene was formed through nucleotide metabolism with immature [GADV]-proteins, primeval AntiC-SL tRNA formation with immature [GADV]-proteins, formations of single-stranded (GNC)n codon sequence by random joining of anticodons, GNCs, carried by AntiC-SL tRNAs, double-stranded (ds)-(GNC)n RNA and maturation of an immature [GADV]-protein, which was produced from one strand of the ds-RNA. Thus, the first gene was formed using the five primitive or prototypes of members, which were produced in the absence of gene. There would be no other way for generating the first gene.

The perturbations of DNA methyltransferase 3 alpha (DNMT3A) may cause uncontrolled gene expression, resulting in cancers and tumors. The DNMT inhibitors Azacytidine (AZA) and Zebu-larine (ZEB) inhibit the DNMT family with no specificities and consequently would bring side effects during the treatment. Therefore, it is vital to understand their inhibitory mechanisms in DNMT3A to inform the new inhibitor design for DNMTs. Herein, we carried out molecular dynamics (MD) and quantum mechanics/molecular mechanics (QM/MM) simulations to investigate the inhibitory mechanisms of AZA and ZEB. The results were compared to the methyl transfer of cytosine. We showed AZA might stop the methyl transfer process, whereas the ZEB might be stuck in a me-thyl-transferred intermediate (IM3). Such IM3 state then fails the elimination due to the unique protein dynamics that result in missing the catalytic water chain. Our results brought atomic-level insights into the mechanisms of the two drugs in DNMT3A, which could benefit the new generation of drug design for the DNMTs.

The paper proves a theorem about the structure of the distribution function on which the extremum of fractionally linear functional is reached in the presence of an uncountable number of linear constraints. The problem of finding an extremal distribution function arises when determining the optimal control strategy in a class of Markov homogeneous randomized control strategies. The structure of extremal functions is described by a finite number of parameters, hence the problem is greatly simplified since it is reduced to the search for an extremum of some function.

The orthogonal matrix with cube be symmetric is a common class of matrices with important properties. We pay attention to this kind of matrices. By using the close relationship between the eigenvalues of a matrix and the trace of its power, we obtain the algorithm for its all possible different eigenvalues and multiplicities. The calculation formula is expressed only by the trace of a matrix and its power, avoiding solving the characteristic polynomial. The method is simple and practical. Furthermore, a new essential characterization for the sum of orthogonal matrix pairs being orthogonal is given as well.