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Preprint ARTICLE | doi:10.20944/preprints202308.0462.v1

Cisplatin Dependent Secretion of Immunomodulatory High Mobility Group Box 1 (HMGB1) Protein from Lung Cancer Cells

Kevin Gillespie, Ross Pirnie, Clementina Mesaros, Ian Blair

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Platinum drugs; acetylation; oxidation; immune response; mass spectrometry; chromatography

Online: 7 August 2023 (10:10:09 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202305.0609.v1

Encephalopathy Caused by Human Parvovirus B19 Genotype 1 Associated With Haemophilus influenzae Meningitis in a Newborn

Noely E. Ferreira, Antonio Charlys Da Costa, Esper Kallas, Cassia G.T. Silveira, Ana Carolina Soares, Layla Honorato, Heuder Gustavo Oliveira Paiao, Silvia Helena Lima, Dewton M. Vasconcelos, Marina F. Côrtes, Silvia Figueiredo Costa, Tania Regina Tozetto-Mendoza, Hélio Rodrigues Gomes, Steven S. Witkin, Maria Cassia Mendes-Correa

Subject: Biology And Life Sciences, Virology Keywords: Human parvovirus B19; Encephalopathy; Metagenomic next-generation sequencing.

Online: 9 May 2023 (08:41:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0163.v1

The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport

Zubaida Hassan, Pretty S. Philip, Bincy Baby, M. Kalim Akhtar, Anatoliy Shmygol, Gulfaraz Khan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: EBER1; Intracellular transport; RPL22; Secretion; Structure

Online: 4 May 2023 (02:48:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0724.v1

PRRSV-1 Stabilization Programmes in French Farrow-to-Finish Farms: a Way to Reduce Antibiotic Usage

Charlotte Teixeira Costa, Pauline Berton, Gwenaël Boulbria, Valérie Normand, Mathieu Brissonnier, Arnaud Lebret

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: porcine reproductive and respiratory syndrome virus; health management; antibiotic usage; pig production sector; PRRSV-1 stabilization program

Online: 21 April 2023 (11:08:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0287.v1

HAX1 Is a Novel Binding Partner of Che-1/AATF. Implications in Oxidative Stress Cell Response

Cinzia Pisani, Annalisa Onori, Francesca Gabanella, Simona Iezzi, Roberta De Angelis, Maurizio Fanciulli, Andrea Colizza, Marco De Vincentiis, Maria Grazia Di Certo, Claudio Passananti, Nicoletta Corbi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: HAX1; Che-1/AATF; apoptosis; mitochondria; oxidative stress; breast cancer; ERα

Online: 17 February 2023 (01:50:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0482.v1

Standard Gibbs Energy of Binding of the gp120 Antigen of HIV-1 to the CD4 Receptor

Marko Popovic

Subject: Biology And Life Sciences, Virology Keywords: Biothermodynamics of viruses; HIV-1; Binding constant; Gibbs energy of binding; Antigen-receptor binding

Online: 25 November 2022 (12:44:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0008.v1

A Multi-agent Approach to Predict Long-Term Glucose Oscillation in Individuals with Type 1 Diabetes

João Paulo Aragão Pereira, Anarosa Alves Franco Brandão, Joyce da Silva Bevilacqua, Maria Lúcia Cardillo Côrrea-Giannella

Subject: Computer Science And Mathematics, Computer Science Keywords: Glucose Oscillation; Prediction; Multi-agent; Type 1 Diabetes; Personalized; Recommendation

Online: 1 September 2022 (07:13:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0282.v1

The Impact of Retinal Configuration on the Protein-Chromophore Interactions in Bistable Jumping Spider Rhodopsin-1

Jonathan Richard Church, Jógvan Magnus Haugaard Olsen, Igor Schapiro

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: Rhodopsins; Bistable; Jumping Spider; QM/MM; Spectral Tuning

Online: 16 November 2021 (09:12:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202108.0503.v1

Characterizations of Graded Rings Over Which Every Graded Semi-primary Ideal is Graded 1-absorbing Primary

Alaa Melhem, Malik Bataineh, Rashid Abu-Dawwas

Subject: Computer Science And Mathematics, Mathematics Keywords: Graded 1-absorbing primary ideals; graded primary ideals; graded semi-primary ideals.

Online: 26 August 2021 (09:57:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202010.0153.v1

Study on the Compressive Behaviour of Sustainable Cement-Based Composites Under 1-Hour Direct Flame Exposure

Ajitanshu Vedrtnam, Chiara Bedon, Gonzalo Barluenga

Subject: Engineering, Automotive Engineering Keywords: Concrete; Cement-Based Composites (CBCs); compressive strength; fire exposure; thermal boundaries; Finite Element (FE) numerical modelling; empirical formulations.

Online: 7 October 2020 (09:27:18 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202010.0063.v1

Photometric Correction of Chang’E-1 Interference Imaging Spectrometer’s (IIM) Limited Observing Geometries data with Hapke Model

Xuesen Xu, Jianjun Liu, Dawei Liu, Bin Liu, Rong Shu

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: CE-1 IIM; photometric correction; Hapke model; observing geometries; opposition surge

Online: 5 October 2020 (10:50:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202001.0122.v1

High PD-1 Expression Level is Associated with an Unfavorable Prognosis in Patients with Cervical Adenocarcinoma

Masako Ishikawa, Kentaro Nakayama, Kohei Nakamura, Hitomi Yamashita, Tomoka Ishibashi, Toshiko Minamoto, Kouji Iida, Sultana Razia, Noriyoshi Ishikawa, Satoru Nakayama, Yoshiro Otsuki, Satoru Kyo

Subject: Physical Sciences, Biophysics Keywords: cervical adenocarcinoma; immune-checkpoint inhibitor; programmed cell death-1(PD-1); programmed cell death-ligand 1(PD-L1); CD8 expression; lymphocyte; survival analysis

Online: 12 January 2020 (15:01:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0341.v1

Near-Real Time Automatic Snow Avalanche Activity Monitoring System Using Sentinel-1 SAR Data in Norway

Markus Eckerstorfer, Hannah Vickers, Eirik Malnes, Jakob Grahn

Subject: Environmental And Earth Sciences, Geophysics And Geology Keywords: SAR; Sentinel-1; snow avalanche; automatic detection

Online: 29 October 2019 (15:37:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0137.v2

Factors Predicting the Oral Health Behaviors of the Iranian Students in the District 1 Tehran, Iran

Mohammad Hossein Delshad, Fatemeh Pourhaji, Alireza Hidarnia, Sedigheh Sadat Tavafian, Shamsodin Niknami

Subject: Social Sciences, Behavior Sciences Keywords: oral health; students; medical; behavior; iran

Online: 11 October 2019 (07:30:00 CEST)

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Aim: The purpose of this examination is determining predictors to oral health behaviors predict in Iranian students in district 1 Tehran based on the health belief model with added commitment to plan construct. Methods: This cross-sectional study were randomly selected conducted on 351 eligible four grade female students in the first district of Tehran, Iran in 2017. The random Multi‑stage random cluster sampling method was used to recruit students. The inclusion criteria were being graded, four female students (aged 9-11 years), or education at the fourth grade of one of the elementary schools studied in the first district of Tehran and, The health of the student from a physical and psychological of view. Logistic regression analysis was used to identify the variables that predict oral health behaviors. To assess the predictors of oral health behaviors, all the expanded Health Belief Model constructs with the construct of “Commitment to Plan of Action” from “Health Promotion Model were examined as risk factors to see if they influence on the probability of brushing behavior and dental floss behavior occurrence and were interpreted through odds ratio (OR). SPSS version 19 was used to analyze the data. Results: Totally 308 four grade female students with mean age of 9.32 ± 0.81 years old took part in the study. The total 31.8% of the students reported that they were brushing behavior less than twice a day and 55.2% students claimed, use of dental floss behavior once a week or less than once a day. The results indicated that perceived self-efficacy (OR=1.46, 95% CI=0.57-3.78, P<0.001), Commitment to plan (OR=1.13, 95% CI=1.04-1.23, P<0.001) and Cues to action (OR=1.42, 95% CI=1.14–1.76, P=0.002) were the significant predicting variables which is the key factor of brushing twice a day, and use of dental floss once a day or more (OR=1.02, 95% CI=0.23-3.53, P=0.003). Conclusion: This study has shown the effectiveness of the health belief model with added commitment to plan construct to predict oral health behavior in female students. So, it seems that the model as a framework for designing training programs to improve students to improve oral health behavior can be used.

Preprint ARTICLE | doi:10.20944/preprints201909.0124.v1

Radiosensitization of HSF-1 Knockdown Lung Cancer Cells by Low Concentrations of Hsp90 Inhibitor NVP-AUY922

Annett Kuehnel, Daniela Schilling, Stephanie Combs, Bernhard Haller, Gabriele Multhoff

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Heat shock factor (HSF-1) knockdown; heat shock proteins 70 and 27; radiosensitization; Hsp90 inhibitor NVP-AUY922; homologous recombination (HR)

Online: 11 September 2019 (13:37:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints201907.0286.v1

Reviewing HIV-1 Gag Mutations in Protease Inhibitors Resistance: Insights for Possible Novel Gag Inhibitor Designs

Chinh Tran-To Su, Darius Wen-Shuo Koh, Samuel Ken-En Gan

Subject: Biology And Life Sciences, Virology Keywords: HIV-1 Gag; Gag inhibitors; Protease; Protease inhibitors; drug resistance mutations; drug design

Online: 25 July 2019 (10:05:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201801.0144.v1

A Significance of MMP-1 in EGFR-TKI Resistant Lung Adenocarcinoma: A Potential of Therapeutic Target

Ryoko Saito, Yasuhiro Miki, Naoya Ishida, Chihiro Inoue, Masayuki Kobayashi, Shuko Hata, Hisafumi Yamada-Okabe, Yoshinori Okada, Hironobu Sasano

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: MMP-1, EGFR-TKI resistance, lung adenocarcinoma, mTOR

Online: 16 January 2018 (13:57:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints201801.0032.v2

The Development of a 1-D Integrated Hydro-Mechanical Model Based on Flume Tests, to Unravel Different Hydrological Triggering Processes of Debris Flows

Theo W.J. van Asch, Bin Yu, Wei Hu

Subject: Environmental And Earth Sciences, Environmental Science Keywords: triggering of debris flows; overland flow; infiltration; laboratory experiments; modelling; rain intensity-duration threshold curves

Online: 13 June 2018 (08:37:32 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1332.v1

Human T-cell Leukemia Virus Type 1 Oncogenesis between Active Expression and Latency: A Possible Source for the Development of Therapeutic Targets

Francesca Marino-Merlo, Sandro Grelli, Antonio Mastino, Michele Lai, Paola Ferrari, Andrea Nicolini, Mauro Pistello, Beatrice Macchi

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HTLV-1; adult T-cell leukemia; viral oncogenesis; Tax; HBZ; apoptosis

Online: 18 August 2023 (09:41:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0561.v1

Immunotherapy with PD-1 Inhibitor Nivolumab in Recurrent/Metastatic Platinum Refractory Head and Neck Cancers: The Early Experience of Two Academic Center

Camil Ciprian Mireștean, Mihai Cosmin Stan, Michael Schenker, Constantin Volovăț, Simona Ruxandra Volovăț, Dragoș Petru Teodor Iancu, Roxana Irina Iancu, Badulescu Florinel

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Nivolumab; immunotherapy; head and neck cancers; HNSCC; chemotherapy; neutrophil to lymphocyt ratio; NLR; second primary malignancy; Cetuximab

Online: 9 May 2023 (04:21:38 CEST)

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The prognosis of patients with recurrent or metastatic of the head and neck squamous-cell carcinoma (HNSCC) refractory to platinum-based chemotherapy is severe and, consequently, the identification of therapeutic options for this category of patients is a priority. Nivolumab, an anti- programmed cell death protein 1 (anti-PD-1) monoclonal antibody, has been approved for the treatment of recurrent or metastatic HNSCC after platinum-based therapy progressing. We present the early experience of two academic center including diagnostic, clinical, biological, therapeutic and outcomes characteristics of head and neck cancer (HNC) patients treated with Nivolumab. The purpose of the study is to identify certain peculiarities and to report them compared to the data from the literature in order to generate some hypotheses that coud constitute criteria for the selection of cases with maximum benefit to immunotherapy. Analyzing the data obtained from 18 patients treated in Emergency County Hospital Craiova, "Saint Nectarie" Oncological Hospital Craiova and Euroclinic Oncological Center Iasi January 2020 and March 2023 it could be hypothesized that lower nadir values of neutrophil to lymphocyte ratio (<3.5) and the adition of intensive regimens of chemotherapy and radiotherapy could justify a favorable response in terms of progression free survival (PFS) during immunotherapy with Nivolumab. To our knowledge, the study included the first two cases of second primary malignancy (SPM) in the head neck region treated with Nivolumab. The reported data support a possible benefit of the sequential administration of radiotherapy, immunotherapy for SPM cases. Higher positive dynamic of neutrophil to lymphocyt ratio (NLR) could be associated with worst outcome during immunotherapy. All study observations including the role of infections and antibiotic treatment as well as the possible biomarker value of NLR variation during immunotherapy should be investigated in multicenter clinical trials.

Preprint ARTICLE | doi:10.20944/preprints202301.0562.v1

Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission. Study 1

Elena Blázquez, Joan Pujols, Fernando Rodriguez, Joaquim Segalés, Rosa Rosell, Joy Campbell, Javier Polo

Subject: Biology And Life Sciences, Virology Keywords: African swine fever; ASFV; spray-dried porcine plasma; challenge; nutritional intervention

Online: 31 January 2023 (02:21:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0170.v1

Low-intensity Pulsed Ultrasound-mediated Blood-brain Barrier Opening Increases Anti-programmed Death-ligand 1 Delivery and Efficacy in Gl261 Mouse Model

Mohammed H Ahmed, Emie Quissac, Nolwenn Lemaire, Isaias Hernández-Verdín, Noël Zahr, Charlotte Schmitt, Guillaume Bouchoux, Michael Canney, M.D. Santin, Alexandra Petiet, Laura Mouton, Coralie Guerin, Lea Guyonnet, Marc Sanson, Maïté Verreault, Alexandre Carpentier, Ahmed Idbaih

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: SonoCloud; GL261 mouse model; ultrasound-mediated drug delivery; glioblastoma; immune checkpoint inhibitors

Online: 9 December 2022 (02:59:28 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202206.0038.v1

Dynamics of Whole Virus and Non-structural Protein 1 (NS1) IgG Response in Mice Immunised with Two Commercial Tick-borne Encephalitis Vaccines

Jiri Salat, Petra Strakova, Daniel Ruzek

Subject: Biology And Life Sciences, Virology Keywords: tick-borne encephalitis virus; vaccine; non-structural protein 1

Online: 3 June 2022 (09:48:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0120.v1

Mathematical Modeling of the Growth Suppression of HER2+ Breast Cancer Colonies Under the Effect of Trastuzumab and PD-1/PD-L1 Inhibitor

Regina Padmanabhan, Hadeel Kheraldine, Ishita Gupta, Nader Meskin, Anas Hamad, Semir Vranic, Ala-Eddin Al Moustafa

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; PD-1/PD-L1; Mathematical model; HER2/PD-1 Interaction; Breast cancer

Online: 9 May 2022 (14:06:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0136.v1

Tick-borne Encephalitis Virus Vaccines Contain Non-structural Protein 1 (NS1) Antigen and May Elicit NS1-Specific Antibody Responses in Vaccinated Individuals

Jiri Salat, Kamil Mikulasek, Osmany Larralde, Petra Pokorna Formanova, Ales Chrdle, Jan Haviernik, Jana Elsterova, Dana Teislerova, Martin Palus, Ludek Eyer, Zbyněk Zdráhal, Juraj Petrik, Daniel Ruzek

Subject: Biology And Life Sciences, Virology Keywords: tick-borne encephalitis; vaccination; NS1; vaccine; flavivirus

Online: 11 February 2020 (09:10:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints201901.0096.v1

Interplay among calcium diet content, PTH(1-34) treatment and balance of bone homeostases in rat model: the trabecular bone as keystone

Marzia Ferretti, Francesco Cavani, Laura Roli, Marta Checchi, Maria Sara Magarò, Jessika Bertacchini, Carla Palumbo

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: calcium diet content; mineral homeostasis; skeletal homeostasis; trabecular bone; PTH(1-34); rat

Online: 10 January 2019 (11:38:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0456.v1

The salt-stress response of the transgenic plum line J8-1 and its interaction with the salicylic acid biosynthetic pathway from mandelonitrile

Agustina Bernal-Vicente, Daniel Cantabella, Cesar Petri, Jose Antonio Hernández, Pedro Díaz-Vivancos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: chlorophyll fluorescence; J8-1 plum line; mandelonitrile; Prunus domestica; redox signalling; salicylic acid; salt-stress; soluble nutrients

Online: 19 October 2018 (14:57:02 CEST)

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Salinity is considered as one of the most important abiotic challenges that affect crop productivity. Plant hormones, including salicylic acid (SA), are key factors in the defence signalling output triggered during plant responses against environmental stresses. We have previously reported in peach a new SA biosynthetic pathway from mandelonitrile (MD), the molecule at the hub of the cyanogenic glucoside turnover in Prunus sp. In this work, we have studied whether this new SA biosynthetic pathway is also present in plum and the possible role this pathway plays in plant plasticity under salinity, focusing on the transgenic plum line J8-1, which displays stress tolerance via an enhanced antioxidant capacity. The SA biosynthesis from MD in non-transgenic and J8-1 micropropagated plum shoots was studied by metabolomics. Then the response of J8-1 to salt stress in presence of MD or Phe (MD precursor) was assayed by measuring: chlorophyll content and fluorescence parameters, stress related hormones, levels of non-enzymatic antioxidants, the expression of two genes coding redox-related proteins, and the content of soluble nutrients. The results from in vitro assays suggest that the SA synthesis from the MD pathway demonstrated in peach is not clearly present in plum, at least under in vitro conditions. Nevertheless, in J8-1 NaCl-stressed seedlings, an increase in SA was recorded as a result of the MD treatment, suggesting that MD could be involved in the SA biosynthesis under NaCl stress conditions in plum plants. We have also shown that the plum line J8-1 was tolerant to NaCl under greenhouse conditions, and this response was somewhat different in MD-treated plants. In that regards, the MD treatment produced an increase in SA, jasmonic acid (JA) and reduced ascorbate (ASC) contents as well as in the coefficient of non-photochemical quenching (qN) and the gene expression of Non-Expressor of Pathogenesis-Related 1 (NPR1) and thioredoxin H (TrxH) under salinity conditions, suggesting a crosstalk between different signalling pathways (NPR1/Trx and SA/JA) leading to salinity tolerance in the transgenic plum line J8-1.

Preprint ARTICLE | doi:10.20944/preprints201701.0050.v1

Interleukin-1 Receptor Antagonist (IL1RA) and Interleukin-4 (IL4) Genes Variable Number Tandem Repeats (VNTRs) Are Associated with Adiposity in Malaysian Subjects

Yung-Yean Kok, Hing-Huat Ong, Yee-How Say

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Interleukin-1 receptor antagonist (IL-1Ra); Interleukin-4 (IL-4); Variable Number Tandem Repeat (VNTR); obesity; adiposity; Malaysia.

Online: 10 January 2017 (10:27:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0500.v1

Thymol@activated Carbon Nanohybrid for Low-Density Polyeth-Ylene Based Active Packaging Films for Pork Fillets Shelf-Life Extension

Aris E. Giannakas, Vassilios K. Karabagias, Dimitrios Moschovas, Areti Leontiou, Ioannis K. Karabagias, Stavros Georgopoulos, Andreas Karydis-Messinis, Konstantinos Zaharioudakis, Nikolaos Andritsos, George Kehayias, Apostolos Avgeropoulos, Charalampos Proestos, Constantinos Ε Salmas

Subject: Chemistry And Materials Science, Nanotechnology Keywords: activated carbon; thymol; low-density polyethylene; active packaging; control release; kinetics; pork fillets; heme iron; shelf life

Online: 7 June 2023 (07:09:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1355.v1

A Comprehensive Review of Electric Vehicles Fast Charging Developments and Technology

Ahmed Taher Zentani, Ali M. A. Almaktoof, MTE Kahn

Subject: Engineering, Electrical And Electronic Engineering Keywords: Electrical vehicles; DC fast charging; power electronics converters; control strategies

Online: 19 April 2024 (16:03:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0031.v1

Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Its Survival on Other Plants including Weed Species

Andrew Chen, Sabrina Morrison, Aphrika Gregson, Duy P. Le, Andrew S. Urquhart, Linda J. Smith, Elizabeth A.B. Aitken, Donald M. Gardiner

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Reporter genes; plant host and pathogen interactions; Green Fluorescent Protein; 'mCherry' Red Fluorescent Protein; alternative weed host; Nicotina benthamiana; Vegetative Compatibility Groups 1A and 2A; Defoliating and non-defoliating pathotypes

Online: 1 March 2024 (10:42:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.1683.v1

Central Actions of Leptin Induce an Atrophic Pattern and Improves Heart Function in Lean Normoleptinemic Rats via PPARβ/δ Activation

Blanca Rubio, Cristina Pintado, Lorena Mazuecos, Marina Benito, Antonio Andrés, Nilda Gallardo

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: PPARβ /δ ; leptin; MRI; cardiac remodeling; Wistar rat

Online: 24 June 2024 (12:03:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0097.v1

Goat Milk: Compositional, Technological, Nutritional, and Therapeutic Aspects

Ahmed R. A. Hammam, Saleh M. Salman, Mohamed S. Elfaruk, Khalid A. Alsaleem

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Goat population; Goat milk; Milk production; Milk composition; Goat cheese; Therapeutic properties

Online: 3 August 2021 (15:57:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201905.0381.v1

Influence of Pore Networking and Electric Current Density on the Crack Pattern in Reinforced Concrete Test Due to Pressure Rust Layer at Early Ages of an Accelerated Corrosion Test

Ángela M. Bazán, Encarnación Reyes, Jaime C. Gálvez

Subject: Engineering, Civil Engineering Keywords: corrosion; concrete cover; cracking; SEM image analysis; rust layer; strain gauge; pore size

Online: 31 May 2019 (08:46:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0821.v1

Psychological, Social, and Behavioral Conditions of Anti-VEGF Patients: Results from the ALBATROS Data Collection

Christian Wolfram, Norbert Pfeiffer, Tobias Hudde, Alexander Klatt, Birthe Schnegelsberg, Mike Ross, Focke Ziemssen, Alexander K Schuster

Subject: Medicine And Pharmacology, Ophthalmology Keywords: Anti-VEGF; patient perspective; attitude; psychology; health care; fear; vision loss; blindness

Online: 13 November 2023 (15:38:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1479.v1

Interplay of Serum Leptin, Tumor Necrosis Factor Alpha, and Testosterone in Overweight-Related Hypertensive Men

Shalan Alaamri, Abdulhalim S Serafi, Zahir Hussain, Shouq K. Bafail, Mohammed A. Bafail, Lusine H. Demirkhanyan, Christopher S Gondi, Sumera Sohail

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Hypertension; overweight-related hypertension; serum leptin; TNF-α, testosterone; total cholesterol; IL-6

Online: 21 June 2023 (08:43:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1716.v1

Archaeal Lipid Vesicles as Immune Modulators, Adjuvants and Vaccine Carriers: A Review of the Past and Current Trends.

Fahime Nasr Esfahani, Seyed Morteza Naghib, Omar Atrooz, Sara Ataei, Gunes Ak, Gokhan Zengin, Mehran Alavi, M. R. Mozafari, and Elham Taghavi

Subject: Biology And Life Sciences, Other Keywords: Adjuvant; Archaeal carriers; Lipid-based drug delivery; Vaccine delivery; Gene therapy; Immune modulators; Tocosome

Online: 28 March 2024 (09:47:09 CET)

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Preprint REVIEW | doi:10.20944/preprints202404.1585.v1

One Health Scoping Review of the Epidemiology of Human Fascioliasis across Africa for the Period 2000–2022

Moses Adriko, Edridah M. Tukahebwa, Miph B. Musoke, David Muwanguzi, Stellah Nambuya, Patrick Vudriko, Kalinda Chester, Samson Mukaratirwa, Moses J. Chimbari, Anna-Sofie Stensgaard, Birgitte Vennervald, Lawrence Mugisha

Subject: Public Health And Healthcare, Other Keywords: human-fascioliasis; prevalence; epidemiology; Africa; One-Health

Online: 25 April 2024 (15:07:38 CEST)

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(1)Background: Fascioliasis is considered a neglected tropical disease affecting an estimated 17 million people in more than 70 countries worldwide. The highest prevalence of human fascioliasis is found in regions where livestock farming and consumption of raw or undercooked freshwater plants are common, such as parts of Africa, Asia, Europe, and Latin America. It is responsible for over 90,000 disability-adjusted years of life due to associated abdominal complications. We conducted a study to investigate the prevalence of human fascioliasis through a systematic review and meta-analysis of all available literature in Sun-Saharan Africa.(2). Methods: We screened a total of 126 publications using pubmed online search engine databases; Web of Science and CAB for articles published within a 20-year period between 2000 and 2022. We used a comprehensive search method using a combination of terms, parasite and disease names. The initial search resulted in a screening of articles by title, abstracts and full texts. The relevant information on the prevalence of human fascioliasis in Africa was extracted. Of the 126 publications, 33 articles were selected for meta-analysis. The essential information required was extracted from these documents and determined through statistical analysis in Microsoft Excel for calculating the prevalence of human fascioliasis in sub-Saharan Africa. The variance of each study was assessed using a bimodal distribution and heterogeneity assessment using a I2 index. Analysis was performed using random effects models.(3)Results: A total of 271 articles were searched through the database search and 33 articles were eligible studies that provided qualitative information on the prevalence of human fascioliasis in Africa. These make up 12.17% (n=33). The pooled prevalence estimates for human fascioliasis are 0.032% (IVhet PPE: 0.032% (95%CI 1.4-6.53). The highest prevalence rate was observed in Egypt with a prevalence of 100%. The prevalence of human fascioliasis is very high in some sub-Saharan countries, partly due to neglect of diagnostics, demonstrating the bias of available information for decision-making in human fascioliasis across Africa, given the spatial presence, scarcity and incompleteness are region.(4)Conclusions: The prevalence of Human fascioliasis is very high in some Sub-Saharan Countries partly due to neglect in diagnostics. This presents how highly focal with spatial occurrences, scarcity and patchy the available information for decision making is on Human fascioliasis across the Sub-Saharan African region.

Preprint ARTICLE | doi:10.20944/preprints201611.0094.v1

Transcription Profile Unveils the Cardioprotective Effect of Aspalathin against Lipid Toxicity

Rabia Johnson, Phiwayinkosi V. Dludla, Christo J.F. Muller, Barbara Huisamen, M. Faadiel Essop, Johan Louw

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: diabetes mellitus; hyperglycemia; cardiomyopathy; lipid toxicity; polyphenols; aspalathin

Online: 17 November 2016 (11:19:37 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1556.v1

Holistic View and Novel Perspective on Ruminal and Extra-gastrointestinal Methanogens in Cattle

Godson Aryee, Sarah M Luecke, Carl R Dahlen, Kendall C Swanson, Samat Amat

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Methanogens; enteric methane emission; cattle; microbiome; holistic; extra-intestinal microbial communities

Online: 25 October 2023 (05:29:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0426.v1

Ecological Civilization COP 15 and Amphibian Sustainability through Reproduction Technologies, Biobanking, and Conservation Breeding Programs (RBCs)

Robert K. Browne, Qinghua Luo, Pei Wang, Nabil Mansour, Svetlana A. Kaurova, Edith N. Gakhova, Natalia V. Shishova, Victor K. Uteshev, Lyudmila I. Kramarova, Rhiannon L. Bolton, Govindappa Venu, Pangda Sopha Sushadi, Somaye Vaissi, Zeynab Taheri-Khas, Pouria Heshmatzad, Mikhail F. Bagaturov, Michelle Hamer, Peter Janzen, Renato E. Naranjo, Aleona Swegen, Julie Strand

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: COP 15; biobanking; amphibian; bioregionalism; intergenerational justice; ART; multilateralism; de-extinction; artificial intelligence; effective altruism

Online: 8 October 2023 (05:23:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1466.v1

A Hardware Realization Framework for Fuzzy Inference System Optimization

Saeid Gorgin, Mohammad Sina Karvandi, Somaye Moghari, Mohammad K Fallah, Jeong-A Lee

Subject: Computer Science And Mathematics, Hardware And Architecture Keywords: Embedded System; Hardware Optimization; MSDF Computing; Fuzzy Inference System

Online: 20 December 2023 (04:24:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202208.0058.v1

Heterogeneity Extends Criticality

Fernanda Sánchez-Puig, Octavio Zapata, Omar K. Pineda, Gerardo Iñiguez, Carlos Gershenson

Subject: Physical Sciences, Theoretical Physics Keywords: complexity; phase transitions; criticality; Ising model; random Boolean networks

Online: 2 August 2022 (09:30:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0446.v1

Who Let the Dogs Out ? Unmasking the Neglected : A Com-prehensive Review on the Enduring Impact of Toxocarosis, a Prevalent Zoonotic Infection

Katrin Henke, Sotirios Ntovas, Aristomenis K. Exadaktylos, Jolanta Klukowska-Rötzler, Mairi Ziaka

Subject: Medicine And Pharmacology, Internal Medicine Keywords: Toxocara canis; Toxocara cati; Toxocarosis; Parasitic infection; Visceral larva migrans; albendazole

Online: 6 June 2023 (11:29:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0737.v1

Interpretable Machine Learning for Assessing the Cumulative Damage of a Reinforced Concrete Frame Induced by Seismic Sequences

Petros C. Lazaridis, Ioannis E. Kavvadias, Konstantinos Demertzis, Lazaros Iliadis, Lazaros K. Vasiliadis

Subject: Engineering, Civil Engineering Keywords: seismic sequence; interpretable machine learning; successive earthquakes; seismic dama-ge prediction; seismic damage accumulation; machine learning; explainable machine learning

Online: 10 May 2023 (10:35:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0396.v1

Feasibility Study of Cable Routing for Bi-Planar Trajectory Tracking in Cable Driven Lower Limb Exoskeleton

Rajan Prasad, Marwan El-Rich, Mohammad I. Awad, Sunil K. Agrawal, Kinda Khalaf

Subject: Engineering, Mechanical Engineering Keywords: cable driven; exoskeleton; lower limb rehabilitation; hip adduction; bi-planar trajectory; optimized routing.

Online: 26 October 2022 (03:54:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0229.v1

Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope

Jiro Akimoto, shinjiro Fukami, Megumi Ichikawa, Kenta nagai, Michihiro Kohno

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: intraoperative photodiagnosis; malignant glioma; fluorescence-guided surgery; intraoperative cytology; fluorescence microscope

Online: 14 September 2021 (10:02:54 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202006.0118.v1

Infliximab Attenuates Paediatric Inflammatory Multisystem Syndrome in SARS-CoV-2 (PIMS-TS)

Joseph Meredith, Cher-Antonia Khedim, Paul Henderson, David C. Wilson, Richard K. Russell

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: paediatric; immunology; infectious disease; gastroenterology

Online: 9 June 2020 (05:11:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0016.v1

Molecular Classification of Colorectal Cancer Using Gene Expression Profile of Tumor Samples

Mamoon Rashid, Ramesh K. Vishwakarma, Ahmad Deeb, Mohamed A. Hussein, Mohammad A. Aziz

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: colorectal cancer; gene expression; molecular classification; molecular subtyping

Online: 2 April 2018 (09:55:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0033.v1

Community Battery for Collective-Self-Consumption and Energy Arbitrage: Techno-Economic Simulations Assessing Energy Balances, Battery Ageing and Different Market Scenarios

Mattia Pasqui, Lorenzo Becchi, Marco Bindi, Matteo Intravaia, Francesco Grasso, Gianluigi Fioriti, Carlo Carcasci

Subject: Engineering, Energy And Fuel Technology Keywords: renewable energy community; battery energy storage system; scheduling; ageing; collective-self-consumption; energy arbitrage

Online: 4 March 2024 (06:12:07 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1746.v1

COVID-19 Variants and Vaccine Development

Ziyao Zhao, Sahra Bashiri, Zyta M. Ziora, Istvan Toth, Mariusz Skwarczynski

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: COVID-19; SARS-CoV-2; Mutations; Variants; Vaccination; Vaccine effectiveness

Online: 24 January 2024 (08:53:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1238.v1

Placental Homeostasis of Oxytocin and Its Ability to Affect the Biological Activity of Retinal Cells

Claudette Adegboro, Wenxiang Luo, Meha Kabra, Ryan M. McAdams, Nathaniel York, R I Wijenayake, Kianna Mary Suchla, De-Ann M Pillers, Bikash Ranjan Pattnaik

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Preterm; retinal vascularization; retinopathy; transcriptomic; connectivity; OXT

Online: 16 September 2024 (14:09:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0846.v1

The State of the Art of Image Guided Superficial Radiation Therapy Treatment of Non-melanoma Skin Cancer in Outpatient Dermatology Clinics and Review of the Literature

Peyton Harris, Aaron Farberg, Janine Hopkins, Liqiao Ma, Donna Serure, Blake Robbins, Candace Osborne, Luis Bravo, Pauline Lausser, Amanda Boatner

Subject: Medicine And Pharmacology, Dermatology Keywords: basal cell carcinoma; image guided superficial radiation therapy; mohs micrographic surgery; non-melanoma skin cancer; squamous cell carcinoma

Online: 13 August 2024 (07:45:45 CEST)

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Introduction Image Guided Superficial Radiation Therapy (IGSRT) is a novel technology which combines traditional superficial radiation therapy (SRT) with high resolution dermal ultrasound (HRDUS) to treat non-melanoma skin cancers (NMSC). Since development, IGSRT use has expanded dramatically in outpatient clinics. We review the IGSRT literature and state of the art operating principles in US dermatologic practices. Materials and Methods A literature search of electronic databases (Medline, Pubmed, Cochrane Library, Science Direct) combined with various authors’ published and unpublished documents, procedures and clinical experience with IGSRT were synthesized for this paper. Results Studies have demonstrated IGSRT consistently delivers high cure rates( >99%) with low complications for early stage (stage 0, I, or II) squamous cell and basal cell carcinomas. Control rates are statistically superior to non-image guided SRT and external beam radiation (XRT) as well as Mohs Micrographic Surgery(MMS). This improvement is attributed to in-vivo dermal tumor visualization via HRDUS and using an interdisciplinary approach to deliver care. IGSRT use in the dermatologic clinic for early stage NMSCs has become common practice and continues to expand. Conclusion While the safety and cosmetic benefits of SRT/XRT has been long documented, IGSRT represents a significant leap forward in efficacy (statistically significant) by adding in-vivo dermal tumor imaging. Results rival and appear on one study to surpass tumor control obtained with MMS. A contributing factor to the success may be the availability and use of an interdisciplinary team approach that includes dermatologists, radiation therapists, radiation oncologists and medical physicists. The high tumor control rates, minimal side effects, favorable cosmesis and ability to treat multiple lesions per session using IGSRT is establishing this modality as a standard first line therapy for early stage NMSCs in dermatology clinics. IGSRT may represent the most effective option for the non-surgical treatment of early stage NMSC to date.

Preprint ARTICLE | doi:10.20944/preprints202305.2069.v1

Antioxidants Prevent Iron Accumulation and Lipid Peroxidation, but do not Correct Autophagy Dysfunction or Mitochondrial Bioenergetics in Cellular Models of BPAN

Alejandra Suárez-Carrillo, Mónica Álvarez-Córdoba, Ana Romero-González, Marta Talaverón-Rey, Suleva Povea-Cabello, Paula Cilleros-Holgado, Rocío Piñero-Pérez, Diana Reche-López, David Gómez-Fernández, José M. Romero-Domínguez, Manuel Munuera-Cabeza, Antonio Díaz, Susana Gonzalez-Granero, José M. García-Verdugo, And José A. Sánchez-Alcázar

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: BPAN; WDR45; antioxidants; autophagy; iron accumulation

Online: 31 May 2023 (03:32:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0901.v1

Sodium Glucose Co-transporter 2 Inhibitors: Pharmacology, Mechanism of Action and Efficacy in Non-diabetic Kidney Disease from Bench to Bed-Side

Aly Mohamed Abdelrahman, Alaa S Awad, Emaad M Abdel-Rahman

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: SGLT2i; CKD; Blood Pressure; IGAN; FSGS; Proteinuria; survival; Non-Diabetics

Online: 11 January 2024 (10:58:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0903.v1

Oleanolic Acid Acetate Alleviates Cisplatin-Induced Nephrotoxicity Via Inhibition of the Caspase and RIPK Pathways

Bori Lee, Yeon-Yong Kim, Seungwon Jeong, Seung Woong Lee, Seung-Jae Lee, Mun-Chual Rho, Sang-Hyun Kim, Soyoung Lee

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: acute kidney injury; oleanolic acid acetate; cisplatin; necroptosis; apoptosis

Online: 15 March 2024 (08:54:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1865.v1

Multimodal Investigation of Metabolism in Murine Breast Cancer Cell Lines Using Fluorescence Lifetime Microscopy and Hyperpolarized 13C-Pyruvate Magnetic Resonance Spectroscopy

Sarah Erickson-Bhatt, Benjamin L. Cox, Erin Macdonald, Jenu V Chacko, Paul Begovatz, Patricia Keely, Suzanne M Ponik, Kevin W Eliceiri, Sean B Fain

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: breast cancer; metastatic potential; metabolism; FLIM; MRSI; hyperpolarized; carbon-13

Online: 25 September 2024 (11:17:44 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0107.v1

From Shallow to Deep Bioprocess Hybrid Modeling: Advances and Future Perspectives

Roshanak Agharafeie, João Rodrigues Correia Ramos, Jorge M Mendes, Rui Oliveira

Subject: Engineering, Bioengineering Keywords: Artificial neural network; Deep learning; Hybrid model; Hybrid neural network; Bioprocess; Digitalization; Industry 4.0

Online: 4 October 2023 (06:52:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0088.v1

Cache Misses and the Recovery of the Full AES 256 Key

Samira Briongos, Pedro Malagón, Juan-Mariano de Goyeneche, Jose M. Moya

Subject: Computer Science And Mathematics, Information Systems Keywords: side-channel cache attacks; cache misses; AES; cloud computing

Online: 11 February 2019 (10:40:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.1528.v1

Novel Fluoride Matrix for Dual‐Range Optical Sensors and Visualization

Alexander A. Alexandrov, Lada A. Petrova, Daria V. Pominova, Igor D. Romanishkin, Maria V. Tsygankova, Sergey V. Kuznetsov, Vladimir K. Ivanov, Pavel P. Fedorov

Subject: Chemistry And Materials Science, Electronic, Optical And Magnetic Materials Keywords: fluoride matrix; luminescence; thermometry; thermography; molten-salt synthesis

Online: 24 July 2023 (03:00:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0484.v1

On Approximate Variational Inequalities and Bilevel Programming Problems

Balendu Bhooshan Upadhyay, I. M. Stancu-Minasian, Subham Poddar, Priyanka Mishra

Subject: Computer Science And Mathematics, Mathematics Keywords: limiting subdifferentials; ϵ-quasi solutions; approximate convex functions; variational inequalities

Online: 8 April 2024 (16:35:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0101.v1

Insights in the Interaction of HPV-16 E6 and Its Variants (E-G350, E-A176/G350, E-C188/G350, AAa and AAc) with MAGI-1

Lilian Esmeralda Araujo- Arcos, Ciresthel Bello-Rios, Olga Lilia Garibay- Cerdenares, Marco Antonio Leyva-Vázquez, Sarita Montaño, Berenice Illades-Aguiar

Subject: Biology And Life Sciences, Biophysics Keywords: E6; variants; MAGI-1; molecular dynamic simulation; docking

Online: 6 September 2021 (13:51:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0108.v1

Bioinformatics-Based Design Strategy and Development of an Antigen Test for SARS-CoV-2 Nucleocapsid Protein

Partha Ray, Melissa Ledgerwood-Lee, Howard Brickner, Alex E. Clark, Aaron Garretson, Rishi Graham, Westley Van-Zant, Aaron F. Carlin, Eliah S. Aronoff-Spencer

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; Nucleocapsid protein; Monoclonal and Polyclonal Antibodies; Enzyme-Linked Immunoassay; Peptide epitope mapping; Viral Quality Assurance; RADx; COVID-19 diagnostics

Online: 1 April 2024 (17:21:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0200.v1

SARS-CoV-2-Specific Immune Responses in Vaccination and Infection during the Pandemic in 2020–2022

Wakana Inoue, Yuta Kimura, Shion Okamoto, Takuto Nogimori, Akane Sakaguchi-Mikami, Takuya Yamamoto, Yasuko Tsunetsugu-Yokota

Subject: Medicine And Pharmacology, Other Keywords: SARS-CoV-2; COVID-19; infection and vaccination; serum and saliva; RBD and N-specific; IgG and IgA; T-cell responses

Online: 5 February 2024 (03:43:25 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202312.1336.v1

Missense Variants in COL4A1/2 Are Associated with Cerebral Aneurysms: A Case Report and Literature Review

Masahiro Uemura, Natsuki Tanaka, Shoichiro Ando, Takehiko Yanagihara, Osamu Onodera

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: COL4A1; COL4A2; cerebral aneurysm; small vessel disease; large vessel abnormality

Online: 18 December 2023 (12:50:43 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1976.v1

SARS‐COV‐2 ORF8 as a Modulator of Cytokine Induction: Evidence and Search for Molecular Mechanisms

Marilia Inês Móvio, Giovana Carneiro, Isabella Lopes Martines, Gilmara Barros de Lima, Sergio Daishi Sasaki, Alexandre Hiroaki KIhara, Emma Poole, Michael Nevels, Maria Cristina Carlan da Silva

Subject: Biology And Life Sciences, Virology Keywords: SARS CoV-2; COVID-19; ORF8, cytokine storm

Online: 30 November 2023 (10:29:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1835.v1

A Portable and Disposable Electrochemical Sensor Utilizing Laser-Scribed Graphene for Rapid SARS-CoV-2 Detection

Runzhong Wang, Bicheng Zhu, Paul Young, Yu Luo, John Taylor, Alan J Cameron, Christopher J. Squire, Jadranka Travas-Sejdic

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: Laser-scribed graphene; biosensor; nanobody; electrochemistry; COVID-19

Online: 29 November 2023 (09:57:13 CET)

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The COVID-19 pandemic caused by the virus SARS-CoV-2 has been the greatest global threat to human health in the last three years. The most widely used methodologies for the diagnosis of COVID-19 are quantitative reverse transcription polymerase chain reaction (RT-qPCR), used to detect the viral genome, and rapid antigen tests (RATs), also known as lateral flow tests, that employ specific antibodies to detect the viral spike protein. While PCR is considered the gold standard test in terms of specificity and sensitivity, it is time-consuming and requires specialized instrumentation operated by skilled personnel. In contrast, RATs can be used in-home or at point-of care but are less sensitive leading to a higher rate of false negative results. An alternative methodology for the detection of virus is the use of electrochemical biosensors that employ disposable strip electrodes to which binding of viral components elicits a change in electrical signal. Laser-scribed graphene (LSG) electrodes are attractive candidates for use as biosensors electrodes suitable for SARS-COV-2 detection due to their low-cost, patternability and ease of fabrication. In this work, we describe the development of a biosensor for COVID-19 detection that exploits a split-ester bond ligase system (termed ‘EsterLigase’) for immobilization of a virus-specific nanobody to maintain the out-of-plane orientation of the probe to ensure the efficacy of the probe-target recognition process. An anti-spike VHH E nanobody, genetically fused with the EsterLigase domain, was used as the specific probe for the spike receptor-binding domain (SP-RBD) protein as the target. The recognition between the two was measured by the change in the charge transfer resistance, determined by fitting the electrochemical impedance spectroscopy (EIS) spectra. The developed LSG-based biosensor achieved a linear detection range for the SP-RBD from 15 pM to 150 nM with a sensitivity of 0.0866 [log(M)]-1 and a limit of detection (LOD) of 7.68 pM.

Preprint ARTICLE | doi:10.20944/preprints202311.1518.v1

Genomic and Phylogenetic Characterisation of SARS-CoV-2 Genomes Isolated in Patients from Lambayeque Region, Peru

Sergio Luis Aguilar-Martinez, Gustavo Adolfo Sandoval-Peña, José Arturo Molina-Mora, Pablo Tsukayama-Cisneros, Cristian Díaz-Vélez, Franklin Rómulo Aguilar-Gamboa, D. Katterine Bonilla-Aldana, Alfonso J. Rodriguez-Morales

Subject: Medicine And Pharmacology, Tropical Medicine Keywords: SARS-CoV-2; genome; sequencing; phylogenetic analysis; Peru

Online: 23 November 2023 (11:07:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1502.v1

Arabinose Plays an Important Role in Regulating the Growth and Sporulation of Bacillus subtilis NCD-2

Yifan Fu, Xiaomeng Liu, Zhenhe Su, Peipei Wang, Qinggang Guo, Ping Ma

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Phenotype Microarrays; Bacillus subtilis; L-arabinose; sporulation; transcriptome

Online: 24 October 2023 (13:28:42 CEST)

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Bacillus subtilis NCD-2 showed a promising biocontrol effect against plant soil-borne diseases, and was developed as a commercial microbial fungicide against cotton verticillium wilt in China. Spores were main ingredient of the fungicide and played the crucial role in biological control of plant diseases. Therefore, the key to reducing the cost of fungicide was to find ways to increase the number of spores of strain NCD-2 during fermentation. In this study, 755 substances were evaluated by Phenotype Microarray technology, and 5 kinds of carbon sources and 1 kind of nitrogen source were found to promote the metabolism of strain NCD-2. Among carbon sources L-arabinose showed strongest ability to promote the bacteria growth and sporulation of strain NCD-2. When L-arabinose was used as a single carbon source, it could increase the bacteria concentration and the sporulation efficiency of strain NCD-2 by 2.04 times and 1.99 times, respectively, compared with D-glucose. Moreover, L-arabinose could significantly decrease the autolysis of strain NCD-2 revealed by microscopy observation. The mechanism for promoting the sporulation by L-arabinose was analysed by RNA-seq sequencing. Results showed that total of 790, 923 and 1270 genes were up-regulated and 639, 850 and 1001 genes were down-regulated under L-arabinose supplies when cultured at 8 h, 12 h and 16 h, respectively. Fourteen differentially expressed genes associated with arabinose transportation and sporulation were selected for qRT-PCR analysis, and the result showed basically consistent expression trend with transcriptome. Notably, genes associated with arabinose metabolism, sporulation, spore resistance to heat and spore coat formation were significantly up-regulated, and genes associated with sporulation-delaying protein were significantly down-regulated under L-arabinose supplies. msmX gene which was involved in arabinose transport in Bacillus genus was deleted, and the mutant decreased the growth and sporulation by 53.71% and 86.46%, respectively, when compared to strain NCD-2 wild type. Complementary of the mutant by importing intact msmX gene could restore the growth and sporulation of the mutant strain. In conclusion, arabinose played an important role in regulating the growth and sporulation of strain NCD-2.

Preprint REVIEW | doi:10.20944/preprints202310.0889.v1

Postural Orthostatic Tachycardia Syndrome Following SARS-CoV-2 Infection and COVID-19 Vaccination: A Comparative Review

Zeinab Mohseni Afshar, Homa Mazaherinia, Hosein Nazari rostami, Arefeh Babazadeh, Mohammad Barary, Erfan Hashemi, Soheil Ebrahimpour

Subject: Public Health And Healthcare, Primary Health Care Keywords: Postural Orthostatic Tachycardia Syndrome (POTS); COVID-19; SARS-CoV-2; Vaccination

Online: 13 October 2023 (10:58:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0859.v1

Comorbidity of Type 2 Diabetes and Dementia Among Hospitalized Patients in Los Angeles County, 2019-2021

D’Artagnan M. Robinson, Dalia Regos-Stewart, Mariana A Reyes, Tony Kuo, Noel C Barragan

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: diabetes; Alzheimer’s disease; dementia; hospitalization

Online: 13 October 2023 (08:26:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0184.v1

The Proinflammatory Effects of 25-Hydroxyvitamin D3 in New-Onset Type 2 Diabetes Mellitus and Prediabetes

Aysen Kutan Fenercioglu, Mustafa Sait Gonen, Hafize Uzun, Nurver Turfaner Sipahioglu, Gunay Can, Ebru Tas, Zehra Kara, Hande Mefkure Ozkaya, Pinar Atukeren

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Prediabetes, type 2 diabetes mellitus, 25-hydroxyvitamin D3, inflammation, interleukins, TNF-α, NF-kB, MAPK

Online: 4 October 2023 (13:09:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1552.v1

Diverse Bioactive Secondary Metabolites from Aspergillus terreus: Antimicrobial, Anticancer and Anti- SARS-CoV-2 Activity Studies

Abdelaaty Hamed, Ahmed S. Abdel-Razek, AHMED B Abdelwahab, Ahmed El Taweel, Mohamed Gaballah, Norbert Sewald, Mohamed Shaaban

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: marine Aspergillus terreus; bioactive metabolites; antimicrobial; cytotoxicity; anti-coronavirus activities

Online: 22 September 2023 (10:07:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0360.v1

SARS-CoV-2 in Domestic UK Cats from Alpha to Omicron: Swab Surveillance and Case Reports

Sarah Jones, Grace B. Tyson, Richard J. Orton, Katherine Smollett, Federica Manna, Kirsty Kwok, Nicolás M Suárez, Nicola Logan, Michael McDonald, Andrea Bowie, Ana Filipe, Brian J. Willett, William Weir, Margaret J. Hosie

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; coronavirus; pets; companion animals; reverse zoonosis; veterinary; animal welfare; feline; One Health

Online: 4 August 2023 (14:48:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0800.v1

Forest Monitoring Based on Sentinel-2 Satellite Imagery, Google Earth Engine Cloud Computing, and Machine Learning

Tamás Molnár, Géza Király

Subject: Biology And Life Sciences, Forestry Keywords: forest monitoring; remote-sensing; satellite image; bark beetle damage; Sentinel-2; vegetation index; water index

Online: 12 July 2023 (11:13:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2087.v1

SARS-CoV-2 and Influenza Vaccine Hesitancy during the COVID-19 Pandemic in a Dynamic Perspective

Valentina Gerussi, Maddalena Peghin, Alvisa Palese, Maria De Martino, Elena Graziano, Stefania Chiappinotto, Federico Fonda, Giulia Bontempo, Tosca Semenzin, Luca Martini, Miriam Isola, Carlo Tascini

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Influenza; COVID-19; SARS-CoV-2; Green-Pass; Vaccination; Immunization; Vaccine-hesitancy; Pandemic

Online: 29 June 2023 (10:23:58 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202306.2007.v1

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega, Klaudia Babisova, Michaela Hyblova, Nikola Janostiakova, Peter Sabaka, Pavol Janega, Gabriel Minárik

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Online: 29 June 2023 (02:12:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1238.v1

A Miniaturized System for Rapid, Isothermal Detection of SARS-CoV-2 in Human and Environmental Samples

Jake Staples, Athanasia-Maria Dourou, Irene Liampa, Calvin Sjaarda, Emily Moslinger, Henry Wong, Prameet Sheth, Stilianos Arhondakis, Ravi Prakash

Subject: Engineering, Bioengineering Keywords: loop-mediated isothermal amplification (LAMP); soft microfluidic chip; Nucleic Acid Tests; severe acute respiratory syndrome-coronavirus 2 (SARS-Cov-2); System-on-board Integrated Detector

Online: 16 June 2023 (12:02:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0226.v1

Zn²⁺ and Cu²⁺ Interaction with the Recognition Interface of ACE2 for SARS-CoV-2 Spike Protein

Alessio Pelucelli, Massimiliano F Peana, Bartosz Orzeł, Elzbieta Gumienna-Kontecka, Serenella Medici, Maria Antonietta Zoroddu

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: ACE2; peptides; zinc complexes; copper complexes; metal interaction; potentiometry; spectroscopy; NMR

Online: 4 May 2023 (08:09:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0008.v1

Screening and Druggability Analysis of Marine Active Metabolites against SARS-CoV-2: An Integrative Computational Approach

Selvakumar Murugesan, Chinnasamy Ragavendran, Amir Ali, Safir Ullah Khan, Velusamy Arumugam, Dinesh Kumar Lakshmanan, Palanikumar Palanichamy, Manigandan Venkatesan, Chinnaperumal Kamaraj, Zia-ur-Rehman Mashwani, Muhammad Younas, Juan Pedro Luna-Arias, Fabian Fernández-Luqueño

Subject: Biology And Life Sciences, Virology Keywords: COVID-19; molecular docking; ADMET; marine natural products; Chrysophaentin A; Hymenidin

Online: 1 December 2022 (03:51:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0390.v1

Determination of SARS-CoV-2 Variants and Seroprevalence in Accra, Ghana during the Delta-Omicron Waves

Irene Owusu Donkor, Elvis Suatey Lomotey, Jewelna Akorli, Millicent Opoku, Emmanuel Frimpong Gyekye, Kojo M. Sedzro, Nana Efua Andoh, Yvonne Ashong, Benjamin Abuaku, Kwadwo Ansah Koram, Vincent Munster

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; asymptomatic; seroprevalence; Delta variant; Omicron variant; vaccination

Online: 26 September 2022 (09:41:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0241.v1

Epidemiological and Clinical Features of SARS-CoV-2 Variants Circulating between April-December 2021 in Italy

Alessia Lai, Annalisa Bergna, Carla Della Ventura, Stefano Menzo, Bianca Bruzzone, Fabio Sagradi, Francesca Ceccherini-Silberstein, Alessandro Weisz, Nicola Clementi, Gaetano Brindicci, Ilaria Vicenti, Lolita Sasset, Sara Caucci, Benedetta Corvaro, Silvia Ippoliti, Carla Acciarri, Vanessa De Pace, Leonardo Lanfranchi, Maria Concetta Bellocchi, Giorgio Giurato, Roberto Ferrarese, Antonella Lagioia, Daniela Francisci, Martina Laura Colombo, Samuel Lazzarin, Matilde Ogliastro, Maria Rosa Cappelletti, Marco Iannetta, Francesca Rizzo, Carmen Rita Santoro, Chiara Fraccalvieri, Sophie Testa, Luca Carioti, Teresa Rocco, Annalisa Saracino, Annamaria Cattelan, Massimo Clementi, Loredana Sarmati, Agostino Riva, Spinello Antinori, Gianguglielmo Zehender

Subject: Biology And Life Sciences, Virology Keywords: variants circulation; SARS-CoV-2; Italy; epidemiology

Online: 16 September 2022 (08:07:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0430.v1

Anticipating the Next Chess Move: Blocking SARS-CoV-2 Replication and Simultaneously Disarming Viral Escape Mechanisms

Samir Mansour Moraes Casseb, André Salim Khayat, Jorge Estefano Santana de Souza, Edivaldo Herculano Correa de Oliveira, Sidney Emanuel Batista dos Santos, Pedro Fernando da Costa Vasconcelos, Paulo Pimentel de Assumpção

Subject: Biology And Life Sciences, Virology Keywords: COVID-19; SARS-CoV-2; siRNA; Treatment

Online: 25 August 2022 (10:00:27 CEST)

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The COVID-19 pandemic initiated a race to determine the best measures to control the disease and to save as many people as possible. Efforts to implement social distancing, the use of masks, and massive vaccination programs turned out to be essential in reducing the devastating effects of the pandemic. Nevertheless, the high mutation rates of SARS-CoV-2 challenge the vaccination strategy and maintain the threat of new outbreaks due to the risk of infection surges and even lethal variations able to resist the effects of vaccines and upset the balance. Most of the new therapies tested against SARS-CoV-2 came from already available formulations developed to treat other diseases, so they were not specifically developed for SARS-CoV-2. In parallel, the knowledge produced regarding the molecular mechanisms involved in this disease was vast due to massive efforts worldwide. Taking advantage of such a vast molecular understanding of virus genomes and disease mechanisms, a targeted molecular therapy based on siRNA specifically developed to reach exclusive SARS-CoV-2 genomic sequences was tested in a non-transformed human cell model. Since coronavirus can escape from siRNA by producing siRNA inhibitors, a complex strategy to simultaneously strike both the viral infectious mechanism and the capability of evading siRNA therapy was developed. The combined administration of the chosen produced siRNA proved to be highly effective in successfully reducing viral load and keeping virus replication under control, even after many days of treatment, unlike the combinations of siRNAs lacking this anti-anti-siRNA capability. Additionally, the developed therapy did not harm the normal cells, which was demonstrated because, instead of testing the siRNA in nonhuman cells or in transformed human cells, a non-transformed human thyroid cell was specifically chosen for the experiment. The proposed siRNA combination deeply reduced the viral load throughout the experiment and allowed cellular recovery, thus representing a potential innovation, to be considered as an additional weapon for therapy of COVID-19 and even other infectious diseases.

Preprint ARTICLE | doi:10.20944/preprints202207.0384.v1

Clinical and Evolutionary Features of SARS Cov-2 Infection (COVID-19) in Children, A Romanian Perspective

George Jugulete, Daniela Pacurar, Mirela Luminita Pavelescu, Mihaela Safta, Elena Gheorghe, Bianca Borcos, Carmen Pavelescu, Mihaela Oros, Madalina Merisescu

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: SARS-CoV-2; COVID-19; children; clinical features; comorbidities; male genderSARS-CoV-2, COVID-19, children, clinical features, comorbidities, male gender

Online: 26 July 2022 (04:43:02 CEST)

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Background: Given the potential for additional development to clarify a better knowledge of the overall impact of COVID-19 on the pediatric population, the clinical symptoms of SARS-CoV-2 infection in children and adolescents are still being explored. Morbidity in children is characterized by a variable clinical course. Our study's goal was to compare clinical aspects of 230 pediatric patients who tested positive for SARS-CoV-2 and were hospitalized between April 2020 and March 2022. Methods: In a retrospective analysis, we compared two groups hospitalized in the infectious diseases clinical ward IX at the National Institute for Infectious Diseases "Prof. Dr. Matei Bals," Bucharest, Romania. The first group of 88 patients was admitted between (April–December 2020) and their clinical manifestations were compared with the second group of 142 children followed between July 2021 and March 2022. Results: Of 230 children, the median age was 4.5 (interquartile range 0.6-17) years, 53.9% were male. 88 (36.21%) patients (first group) were admitted during the second wave in Romania, mostly aged < 5 years old, and experienced digestive manifestations like fever (p=0.001), and diarrhoea (p=0.004). The second group experienced different clinical signs when compared with the first group, with higher temperature and increased respiratory symptoms analogous to those of acute respiratory viral infections. The proportion in the second group increased, and 64.5% had symptoms for a median interval of 5 days; age (0-4 -years old) and length of stay were both proportionally inversely (p<0.01) and with correlation with hospital admission (p = 0.04). We report two Paediatric Inflammatory Multisystem Syndrome (PIMS) in the second group, with favourable evolution under treatment. Comorbidities were risk factors for complications appear (p < 0.001) in both groups. All paediatric cases admitted to our clinic evolved favourably and no death was recorded. Conclusions: In the first group children experienced digestive symptoms, whereas the second group experienced mild and moderate respiratory symptoms. We confirmed risk factors for severe cases as manifestations across the age spectrum, 0-4 (digestive symptoms) and 5-12 years old (for respiratory symptoms), associated comorbidities, fever, and male gender. The potential effects of COVID-19 infection in children older than 5 years should encourage caregivers to vaccinate and improve the prognosis among pediatric patients at risk.

Preprint ARTICLE | doi:10.20944/preprints202207.0380.v1

Molecular Mimicry between SARS-CoV-2 and Human Endocrinocytes: A Prerequisite of Post-COVID Endocrine Autoimmunity

Leonid P. Churilov, Muslimbek G. Normatov, Vladimir J. Utekhin

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV-2; COVID-19; autoantibodies; autoimmune endocrinopathies; long-COVID syndrome; molecular mimicry; thyroid gland; adrenals; pituitary; Langerhans’ islets

Online: 26 July 2022 (03:42:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0155.v1

Susceptibility of SARS-CoV-2 Omicron Variants to Therapeutic Monoclonal Antibodies: Systematic Review and Meta-Analysis

Kaiming Tao, Philip L. Tzou, Sergei L. Kosakovsky Pond, John P.A. Ioannidis, Robert W. Shafer

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; Omicron variant; Monoclonal antibody; Neutralization; Spike protein

Online: 10 March 2022 (14:30:25 CET)

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Preprint REVIEW | doi:10.20944/preprints202105.0264.v1

Applying Philosophy, Logic, and Rational Argumentation to the Severe Acute Respiratory Syndrome Coronavirus-2 Pandemic Response

Ari Joffe, David Redman

Subject: Social Sciences, Anthropology Keywords: COVID-19; critical scrutiny; logic; pandemic; response; SARS-CoV-2

Online: 12 May 2021 (10:48:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Working Paper ARTICLE

XEN Glaucoma Implant for Refractory Glaucoma Management: Results and Complications during a 2-year Follow-up

Katarzyna Lewczuk, Joanna Konopińska, Joanna Joablonska, Zofia Mariak, Marek Rękas

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: refractory glaucoma; XEN implant; management; retrospective study, MIGS, glaucoma surgery, bleb, subconjunctival stent

Online: 2 March 2021 (14:25:54 CET)

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Preprint REVIEW | doi:10.20944/preprints202102.0432.v1

Biomedical Research During the COVID-19 Pandemic: Success in Sars-Cov-2 and Other Infectious Diseases

Nuno S. Osório, Maria Isabel Veiga

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: COVID-19; SARS-CoV-2; Infectious disease; Pandemics; Epidemics; Biomedical Research

Online: 19 February 2021 (10:03:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202009.0345.v1

ACEII Gene Analysis Exposes SARS-CoV-2 As A Potential Threat to Agricultural and National Security

Michael Ruhl, Tracie Jenkins

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: food safety; SARS-CoV-2; coronavirus; ACEII; agricultural security; national security

Online: 16 September 2020 (03:57:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0293.v1

PhotonLabeler: An Inter-disciplinary Platform for Visual Interpretation and Labeling of ICESat-2 Geolocated Photon Data

Lonesome Malambo, Sorin C. Popescu

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: ICESat-2; photon-counting lidar; photon labeling; visualization; ATL03; ATL08; visual interpretation; solar-induced noise

Online: 13 August 2020 (08:08:40 CEST)

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Preprint REVIEW | doi:10.20944/preprints202006.0086.v2

An Overview of Basic Molecular Biology of SARS-CoV-2 and Current COVID-19 Prevention Strategies

Ankur Das, Raja Ahmed, Suraiya Akhtar, Khaleda Begum, Sofia Banu

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; Genome organisation and expression; Polyproteins; Prevention strategies

Online: 14 June 2020 (16:49:10 CEST)

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Preprint REVIEW | doi:10.20944/preprints202005.0376.v1

Computational Strategies to Combat COVID-19: Useful Tools to Accelerate SARS-CoV-2 and Coronavirus Research

Franziska Hufsky, Kevin Lamkiewicz, Alexandre Almeida, Abdel Aouacheria, Cecilia Arighi, Alex Bateman, Jan Baumbach, Niko Beerenwinkel, Christian Brandt, Marco Cacciabue, Sara Chuguransky, Oliver Drechsel, Robert D. Finn, Adrian Fritz, Stephan Fuchs, Georges Hattab, Anne-Christin Hauschild, Dominik Heider, Marie Hoffmann, Martin Hölzer, Stefan Hoops, Lars Kaderali, Ioanna Kalvari, Max von Kleist, René Kmiecinski, Denise Kühnert, Gorka Lasso, Pieter Libin, Markus List, Hannah F. Löchel, Maria J. Martin, Roman Martin, Julian Matschinske, Alice C. McHardy, Pedro Mendes, Jaina Mistry, Vincent Navratil, Eric Nawrocki, Áine Niamh O'Toole, Nancy Palacios-Ontiveros, Anton I. Petrov, Guillermo Rangel-Piñeros, Nicole Redaschi, Susanne Reimering, Knut Reinert, Alejandro Reyes, Lorna Richardson, David L. Robertson, Sepideh Sadegh, Joshua B. Singer, Kristof Theys, Chris Upton, Marius Welzel, Lowri Williams, Manja Marz

Subject: Biology And Life Sciences, Virology Keywords: virus bioinformatics; SARS-CoV-2; sequencing; epidemiology; drug design; tools

Online: 23 May 2020 (11:01:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202004.0510.v1

A Structural View at SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping

Maria Romano, Alessia Ruggiero, Flavia Squeglia, Giovanni Maga, Rita Berisio

Subject: Biology And Life Sciences, Biophysics Keywords: SARS-CoV-2; COVID19; RNA replication; protein structure; infectious disease

Online: 29 April 2020 (12:52:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202004.0425.v1

Non-Coding RNAs and Innovative Therapeutic Strategies to Target the 5’UTR of SARS-CoV-2

Antonella Baldassarre, Alessandro Paolini, Stefania Paola Bruno, Cristina Felli, Alberto Eugenio Tozzi, Andrea Masotti

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SARS-CoV-2; COVID-19; 5’UTR; miRNAs; RNAi; GapmeRs

Online: 24 April 2020 (04:16:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202004.0377.v1

The Dynamics of Humoral Immune Responses Following SARS-CoV-2 Infection and the Potential for Reinfection

Paul Kellam, Wendy Barclay

Subject: Biology And Life Sciences, Virology Keywords: COVID-19; SARS-CoV-2; immune response; antibodies

Online: 21 April 2020 (06:56:12 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.1879.v3

A Comprehensive Review on Phage Therapy and Phage-Based Drug Development

Longzhu Cui, Shinya Watanabe, Kazuhiko Miyanaga, Kotaro Kiga, Teppei Sasahara, Yoshifumi Aiba, Xin-Ee Tan, Srivani Veeranarayanan, Kanate Thitiananpakorn, Huong Minh Nguyen, Dhammika Leshan Wannigama

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: bacteriophage; phage therapy; antimicrobial resistance; intracellular pathogen; biofilm generating pathogen; phage vector; antibacterial phage capsid; phage-based vaccines; phage-based medicine.

Online: 9 September 2024 (11:38:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1690.v1

Generating Renewable Energy Based on a Circuit Approach (Part 2)

Shinichi Ishiguri

Subject: Physical Sciences, Applied Physics Keywords: current source; voltage source; electric power; renewable energy; circuit; light-emitting diode; super-condenser

Online: 23 August 2024 (09:17:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0434.v1

Inhibition of Protease Activated Receptor-2 Activation in Parkinson’s Disease

Santina Quarta, Michele Sandre, Mariagrazia Ruvoletto, Marta Campagnolo, Aron Emmi, Alessandra Biasiolo, Patrizia Pontisso, Angelo Antonini

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Neuroinflammation; Microglia; Neurodegenerative diseases; PAR2 inhibition

Online: 4 July 2024 (11:05:00 CEST)

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