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Preprint BRIEF REPORT | doi:10.20944/preprints202409.2320.v2

Gene Therapy Approach for Treatment of Obese Agouti Mice

Maxim A. Yunin, Stanislav S. Boychenko, Petr Lebedev, Alexey Deykin, Mikhail Pokrovsky, Alexander D. Egorov

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: obesity; agouti mice; lipid profiling; TAG; mass spectrometry

Online: 24 October 2024 (03:14:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201608.0084.v2

Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry

Xiaowei Fang, Shuiping Yang, Konstantin Chingin, Liang Zhu, Xinglei Zhang, Zhiquan Zhou, Zhanfeng Zhao

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: extractive electrospray ionization; rapid detection; malachite green; water; mass spectrometry

Online: 11 August 2016 (10:00:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1057.v1

Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 in C. reinhardtii

Laura Mosebach, Shin-Ichiro Ozawa, Muhammad Younas, Huidan Xue, Martin Scholz, Yuichiro Takahashi, MIchael Hippler

Subject: Biology And Life Sciences, Plant Sciences Keywords: photosystem I; chemical crosslinking; mass spectrometry; Chlamydomonas reinhardtii

Online: 16 April 2024 (15:57:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1802.v1

First-Trimester Preeclampsia-Induced Disturbance in Maternal Blood Serum Proteome: A Pilot Study

Natalia Starodubtseva, Alisa Tokareva, Alexey Kononikhin, Alexander Brzhozovskiy, Anna Bugrova, Evgenii Kukaev, Kamilla Muminova, Alina Nakhabina, Vladimir E. Frankevich, Evgeny Nikolaev, Gennady Sukhikh

Subject: Biology And Life Sciences, Life Sciences Keywords: preeclampsia; prognosis; quantitative proteomics; screening; mass spectrometry; serum; pregnancy

Online: 27 August 2024 (04:34:32 CEST)

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Preeclampsia (PE) is a complex and multifaceted obstetric syndrome characterized by several distinct molecular subtypes. It complicates up to 5% of pregnancies and significantly contributes to maternal and newborn morbidity, thereby diminishing the long-term quality of life for affected women. Due to the widespread dissatisfaction with the effectiveness of existing approaches for assessing PE risk, there is a pressing need for ongoing research to identify newer, more accurate predictors. This study aimed to investigate early changes in the maternal serum proteome and associated signaling pathways. The levels of 125 maternal serum proteins at 11-13 weeks of gestation were quantified using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM MS) with the BAK-125 kit. Ten serum proteins emerged as potential early markers for PE: Apolipoprotein M (APOM), Complement C1q subcomponent subunit B (C1QB), Lysozyme (LYZ), Prothrombin (F2), Albumin (ALB), Zinc-alpha-2-glycoprotein (AZGP1), Tenascin-X (TNXB), Alpha-1-antitrypsin (SERPINA1), Attractin (ATRN), and Apolipoprotein A-IV (APOA4). Notably, nine of these proteins have previously been associated with PE in prior research, underscoring the consistency and reliability of our findings. These proteins play key roles in critical molecular processes, including complement and coagulation cascades, platelet activation, and insulin-like growth factor pathways. To improve the early prediction of PE, a highly effective Support Vector Machine (SVM) model was developed, analyzing 19 maternal serum proteins from the first trimester. This model achieved an Area Under the Curve (AUC) of 0.91, with 87% sensitivity and 95% specificity, and a hazard ratio (HR) of 13.5 (4.6 - 40.8) with p<0.001. These findings demonstrate that serum protein-based SVM models possess significantly higher predictive power compared to the routine first-trimester screening test, highlighting their superior utility in the early detection and risk stratification of PE.

Preprint ARTICLE | doi:10.20944/preprints202404.0567.v1

Immunogenicity of Non-mutated Ovarian Cancer-Specific Antigens

Leslie Hesnard, Catherine Thériault, Maxime Cahuzac, Chantal Durette, Krystel Vincent, Marie-Pierre Hardy, Joël Lanoix, Gabriel Ouellet Lavallée, Juliette Humeau, Pierre Thibault, Claude Perreault

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Tumor-specific antigen; Vaccine; Mass spectrometry; Antigen presentation; Immunogenicity; Dendritic cells

Online: 8 April 2024 (15:41:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1326.v1

Diagnostic Value of Menstrual Blood Lipidomics in Endometriosis: A Pilot Study

Natalya Starodubtseva, Vitaliy Chagovets, Alisa Tokareva, Madina Dumanovskaya, Evgenii N. Kukaev, Anastasia Novoselova, Vladimir Frankevich, Stanislav V. Pavlovich, Gennady Tikhonovich Sukhikh

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: lipid; endometriosis; dried blood spot; mass-spectrometry; diagnostic

Online: 21 May 2024 (17:22:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0937.v1

COF-SiO2@Fe3O4 Core-Shell Composite for Magnetic Solid-Phase Extraction of Pyrethroid Pesticides in Vegetables

Ling Yu, Aiqing Xia, Yongchao Hao, Weitao Li, Cuijuan Xing, Zan Shang, Yiwei Zhang

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: covalent organic framework; magnetic solid-phase extraction; gas chromatography-mass spectrometry; pyrethroids pesticides

Online: 19 February 2024 (11:28:09 CET)

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Pyrethroid pesticides (PYRs) as the third most widely used pesticides, following organophosphorus and carbamate pesticides, which are of significant importance in the analysis and detection of vegetables. However, the current pretreatment technology for PYRs confronts challenges of difficult separation and low enrichment efficiency, resulting in a cumbersome and time-consuming pretreatment process with poor selectivity. Here, a simple and efficient magnetic solid-phase extraction (MSPE) strategy was developed to simultaneously purify and enrich five PYRs in vegetables, with the magnetic covalent organic framework nanomaterial COF-SiO2@Fe3O4 as an adsorbent. The COF-SiO2@Fe3O4 was prepared by a simple solvothermal conditions method, using Fe3O4 as magnetic core, benzidine and 3,3,5,5-tetraaldehyde biphenyl as two building units. COF-SiO2@Fe3O4 could effectively capture the targeted PYRs by virtue of its abundant π-electron system and hydroxyl groups. The impact of various experimental parameters on extraction efficiency was investigated to optimize the MSPE conditions, including adsorbent amount, extraction time, elution solvent type and elution time. Subsequently, method validation was conducted under the optimal conditions in conjunction with gas chromatography-mass spectrometry (GC-MS). Within the range of 5.00–100 μg·kg-1 (1.00–100 μg·kg-1 for bifenthrin and 2.5–100 μg·kg-1 for fenpropathrin), the five PYRs exhibited a strong linear relationship, with determination coefficients ranging from 0.9990 to 0.9997. The limits of detection (LODs) were 0.3–1.5 μg·kg-1, and the limits of quantification (LOQs) were 0.9–4.5 μg·kg-1. The recoveries were 80.2–116.7% with relative standard deviations (RSDs) below 7.0%. Finally, COF-SiO2@Fe3O4, NH2-SiO2@Fe3O4 and Fe3O4 were compared as MSPE adsorbents for PYRs. The results indicated that COF-SiO2@Fe3O4 was an efficient and rapid selective adsorbent for PYRs. This method holds promising prospects for the determination of PYRs in real samples.

Preprint ARTICLE | doi:10.20944/preprints202409.1572.v1

Dietary Regulation of Lipid Metabolism in Gestational Diabetes Mellitus: Implications for Fetal Macrosomia

Natalya Frankevich, Vitaliy Chagovets, Alisa Tokareva, Natalia Starodubtseva, Elizaveta Limonova, Gennady Sukhikh, Vladimir Frankevich

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: macrosomia; gestational diabetes mellitus; obesity; lipidome; mass-spectrometry; diet therapy

Online: 20 September 2024 (04:46:16 CEST)

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The primary therapeutic method for correcting hyperglycemia today is diet therapy. Lipids are not only a source of nutrients but can also act as initiators of adipocyte differentiation in the fetus, which explains the development of fetal macrosomia and future metabolic disorders in children born to mothers with gestational diabetes mellitus (GDM). Changes in the profile of circulating lipids in the maternal bloodstream, depending on adherence to a rational diet among mothers with GDM and the development of fetal macrosomia, is a complex and not fully understood process. The aim of the study was to examine the characteristics of the lipid profile in the blood plasma of pregnant women with GDM in all trimesters of pregnancy based on adherence to diet therapy. The design of the clinical part of the work was carried out within the framework of a 'case-control' study.The study included 110 women, 80 of whom were in the control group, 20 in the group with GDM adhering to the diet, and 10 in the group with GDM not adhering to the diet. The laboratory part was conducted as part of a longitudinal dynamic study with venous blood samples taken at three points: 11-13, 24-26, and 30-32 weeks of pregnancy.A significant impact of diet therapy on the blood lipid composition throughout pregnancy was demonstrated, starting as early as the first trimester. ROC analysis showed high effectiveness of the developed models with an AUC of 0.98 for the "30-32 weeks" model, with sensitivity and specificity of 1 and 0.9, respectively. An association was found between diet, maternal blood lipid composition at 32 weeks of pregnancy, and newborn weight. The direction of changes in the lipid spectrum during the development of macrosomia and diet therapy is diametrically opposed. This confirms at the molecular level that diet therapy can normalize not only carbohydrate but also lipid metabolism in the mother and fetus. Based on the obtained data, it can be suggested that after validation, the developed models could be used to refine the prognosis of macrosomia development based on the lipid profile of blood plasma at different stages of pregnancy.

Preprint ARTICLE | doi:10.20944/preprints202409.0325.v1

A Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar

Basharat Ali, Anshu Chauhan, Mohit Kumar, Praveen Kumar, Hans Carolus, Celia Lobo Romero, Rudy Vergauwen, Ashutosh Singh, Shivaprakash M. Rudramurthy, Patrick Van Dijck, Ashraf S Ibrahim, Rajendra Prasad

Subject: Biology And Life Sciences, Life Sciences Keywords: mucormycosis; drug resistance; posaconazole; amphotericin B; sphingolipids; phospholipids; sterols; mass-spectrometry

Online: 4 September 2024 (08:26:00 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202407.2071.v1

Proteomics and PTMomics to Unveil Metabolic Regulation

Hong Zhang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: quantitative proteomics; mass spectrometry; post translational modifications (PTMs); acetylation; succinylation; lactylation

Online: 26 July 2024 (08:24:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1801.v2

Development and Validation of an Analytical Method for the Simultaneous Detection of Sulfonamides, Trimethoprim and Dapsone in Honey by Liquid Chromatography-Tandem Mass Spectrometry

Gabriela Valentina Vesa, Alexandra-Veronica Luca, Anette Iudit Gheiti-Heghedus, Liora Mihaela Colobatiu, Ana-Andreea Cioca, Maria-Magdalena Poenaru, Marian Mihaiu

Subject: Public Health And Healthcare, Other Keywords: analytical method; antibiotic residues; sulfonamides; trimethoprim; dapsone; honey; liquid chromatography-tandem mass spectrometry

Online: 25 March 2024 (11:17:28 CET)

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Preprint REVIEW | doi:10.20944/preprints202406.1731.v1

Identification of Plant Compounds from Mass Spectrometry Imaging (MSI)

Nancy Shyrley García-Rojas, Carlos Daniel Sierra-Álvarez, Hilda E. Ramos-Aboites, Abigail Moreno-Pedraza, Robert Winkler

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: plant metabolomics; mass spectrometry imaging; compound identification

Online: 25 June 2024 (09:24:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0309.v1

From Lipid Signatures to Cellular Responses: Unraveling the Complexity of Melanoma and Furthering Its Diagnosis and Treatment

Elisa Díaz-Grijuela, Agustín Hernández, Claudia Caballero, Roberto Fernandez, Raquel Urtasun, Marina Gulak, Egoitz Astigarraga, Miguel Barajas, Gabriel Barreda-Gómez

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: ; melanoma; comparative oncology; liquid biopsy; mass spectrometry; lipidomics ;

Online: 3 July 2024 (07:11:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1288.v1

Antioxidant Activity and UHPLC-MS/MS Characterization of Polyphenol and Nicotine Content of Nicotiana glauca Leaf Extracts: A Comparative Study of Conventional and Deep Eutectic Solvent Extraction Methods

Reem Issa, Faisal Al-Akayleh, Lilian Alnsour, Tabarak Alsammaraie, Khaled W. Omari, Shady Helmi Awwad

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Nicotiana glauca; Chromatography; Mass Spectrometry; Antioxidants; deep eutectic solvents; sustainable extraction.

Online: 19 June 2024 (16:31:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0921.v1

Glyphosate as Contaminant in Cereals: Chromatographic Methods for Its Determination. Discussion about Occurrence, Accumulation, Fate, Degradation, and Regulatory Status

Maurizio Masci, Roberto Caproni, Teresina Nevigato

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: glyphosate; analytical methods; sample preparation; liquid chromatography; gas chromatography; mass spectrometry; cereals; occurrence; accumulation mechanism; regulatory status

Online: 15 March 2024 (12:14:02 CET)

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The European Union's recent decision to renew the authorization for the use of glyphosate until 15 December 2033 stimulates the scientific discussion all around the world regarding its toxicity or otherwise for humans. Glyphosate is a chemical used by millions of tons in the last 50 years worldwide to drying weeds in cultivated fields, greenhouses, roadsides. Concern has been raised in many quarters about the possible presence in the food chain and the consequent adverse effects on health: both aspects that argue in favor of toxicity and those that instead may indicate limited toxicity of glyphosate are here discussed. The great debate that has been generated requires further investigations and field measurements to understand its fate once dispersed in the environment and its concentration in the food chain. Hence the need for validated analytical methods that are available to analysts in the field. In the present review, methods for the analytical determination of glyphosate and its main metabolite, AMPA, are discussed, with a specific focus on chromatographic techniques applied to cereal products. The experimental procedures are explained in any detail, including cleanup, derivatization, and instrumental conditions to give the laboratories involved enough information to proceed with the implementation of this line of analysis. The prevalent chromatographic methods used are LC-MS/MS, GC-MS/SIM, and GC-MS/MS but sufficient indications are also given to those laboratories that wish to use the simpler HPLC-FLD, HPLC-UV, GC-NPD or GC-FPD techniques for screening purposes. Concentrations from literature measured in wheat, corn, barley, rye, oats, soybean, and cereal-based foods are reported together with the regulatory status in various parts of the world and the accumulation mechanism. As for the accumulation in cereals, available data show that glyphosate tends to accumulate more in wholemeal flours than in refined ones, that its concentration in the product strictly depends on the treatment period (the closer it is to the time of harvesting, the higher the concentration) and that in cold climates the herbicide tends to persist in the soil for a long time.

Preprint ARTICLE | doi:10.20944/preprints202410.2036.v1

Chemical Investigation of the Mediterranean Sponge Crambe crambe by UHPLC-HRMS/MS via manual and computational dereplication approaches

Pinelopi Vlachou, Nikolaos Tsafantakis, Nikola Milic, Alexandros Polyzois, Eirini Baira, AIkaterini Termentzi, Géraldine Le Goff, Jamal Ouazzani, Nikolas Fokialakis

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Crambe crambe; UHPLC-HRMS/MS; dereplication; computational mass spectrometry; molecular networking; guanidine alkaloids; crambescin analogues

Online: 28 October 2024 (08:20:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.0368.v1

Regulated ICP-OES Detectible Elements in Utah Lake: Characterization and Discussion

Rachel Ann Valek, Kaylee Brooke Tanner, Jacob B Taggert, Rebecca Lynne Ryan, Anna Catherine Cardall, Lauren M. Woodland, Maddeline J. Tanner, Gustavious Paul Williams, A. Woodrull Miller, Robert B. Sowby

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: Utah Lake; Inductively Coupled Plasma Optical Emission Spectrometry; water quality; micronutrient; lake management; trace elements; state regulations

Online: 4 July 2024 (09:16:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0338.v1

Impact of Gut Microbiota and SCFAs in PCOS Pathogenesis and Metformin on Therapy

Evgenii Kukaev, Ekaterina Kirillova, Alisa Tokareva, Elena Rimskaya, Natalia Starodubtseva, Galina Chernukha, Tatiana Priputnevich, Vladimir Frankevich, Gennady Sukhikh

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: metabolomics; polycystic ovary syndrome; short-chain fatty acids; pathogenesis; intestinal microbiota; gas chromatography–mass spectrometry

Online: 4 September 2024 (08:42:49 CEST)

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Polycystic ovary syndrome (PCOS) is a complex disorder that impacts both the endocrine and metabolic systems. The gut microbiota plays a crucial role in in metabolism regulation and immune responses through its interaction with the external environment. This article explores the intricate interplay between intestinal flora and the pathogenesis of PCOS, focusing on the pivotal role of microbial metabolites, specifically short-chain fatty acids (SCFAs). SCFA levels in fecal and blood samples from women with PCOS (n=69) and healthy controls (n=18) were analyzed using Gas Chromatography-Mass Spectrometry (GC/MS) for precise measurement. The results revealed that women with PCOS exhibited a significant reduction in beneficial bacteria alongside a notable overgrowth of opportunistic microorganisms. An overproduction of acetic acid (AA) and valeric acid (VA) in fecal samples suggests a link between elevated SCFAs and the development of obesity and PCOS. Interestingly, AA in the bloodstream might offer a protective effect against PCOS by ameliorating key symptoms such as high body mass index (BMI), insulin resistance (IR), and chronic inflammation. This finding points to the potential therapeutic value of maintaining optimal AA levels in the blood. Although serum SCFA levels showed non-significant changes following metformin treatment, the normalization of AA in the gut underscores that metformin exerts a more pronounced effect locally within the gastrointestinal tract. This treatment also led to an increase in beneficial gut bacteria and a reduction in opportunistic pathogens, suggesting a restoration of gut microbial balance. Furthermore, the study identified the most effective model for predicting the success of metformin therapy, based on serum concentrations of butyric acid (BA) and VA, achieving a 91% accuracy rate, 100% sensitivity, and 80% specificity. These promising findings highlight the potential for developing targeted interventions and personalized treatments, ultimately improving clinical outcomes for women with PCOS.

Preprint ARTICLE | doi:10.20944/preprints202104.0445.v1

Phase Correction for Absorption Mode Two-dimensional Mass Spectrometry

Marc-André Delsuc, Kathrin Breuker, Maria A. van Agthoven

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: mass spectrometry; two-dimensional; tandem mass spectrometry; phase correction; absorption mode; data processing

Online: 16 April 2021 (12:41:15 CEST)

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Working Paper ARTICLE

Novel Cytoskeleton-Associated Proteins in Trypanosoma brucei are Essential for Cell Morphogenesis and Cytokinesis

Marina Schock, Steffen Schmidt, Klaus Ersfeld

Subject: Biology And Life Sciences, Insect Science Keywords: Trypanosoma brucei; cytoskeleton; microtubules; BioID; mass spectrometry

Online: 1 October 2021 (14:04:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1331.v1

Chiral Trapped Headspace GC-QMS-IMS: Boosting Untargeted Benchtop Volatilomics to the Next Level

Lukas Bodenbender, Sascha Rohn, Simeon Sauer, Markus Jungen, Philipp Weller

Subject: Biology And Life Sciences, Food Science And Technology Keywords: HS-GC-MS-IMS; ion mobility spectrometry; volatile organic compounds; trapped headspace sampling; authentication; mango puree; chemometrics

Online: 16 July 2024 (13:01:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0762.v1

High-Throughput Drug Stability Assessment via Biomimetic Metalloporphyrin Catalyzed Reactions Using Laser-Assisted Rapid Evaporative Ionization Mass Spectrometry (LA-REIMS)

András Marton, Zsombor Márton Mohácsi, Balázs Decsi, Balázs Csillag, Júlia Balogh, Richárd Schäffer, Tamás Karancsi, György Tibor Balogh

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: high-throughput screening; metabolic stability; ambient mass spectrometry; LA-REIMS; biomimetic metalloporphyrin; lead profiling; in vitro models; drug metabolism

Online: 12 June 2024 (14:15:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0056.v1

Untangling the Complexities of Processing and Analysis for Untargeted LC-MS Data Using Open-source Tools

Εlizabeth J. Parker, Kathryn C. Billane, Nichola Austen, Anne Cotton, Rachel M. George, David Hopkins, Janice A. Lake, James K. Pitman, James N. Prout, Heather J. Walker, Alex Williams, Duncan D. Cameron

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: metabolomics; untargeted; mass-spectrometry; open-source; bioinformatics

Online: 3 February 2023 (04:16:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints201906.0088.v1

LC-HRMS Screening and Identification of Novel Peptide Markers of Ricin Based on Multiple Protease Digestion Strategies

Long-Hui Liang, Chang-Cai Liu, Bo Chen, Long Yan, Hui-Lan Yu, Yang Yang, Ji-Na Wu, Xiao-Sen Li, Shi-Lei Liu

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: ricin; marker peptides; unambiguous identification; mass spectrometry

Online: 11 June 2019 (08:27:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0242.v1

Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

Izabela Nawrot-Hadzik, Sylwester Ślusarczyk, Sebastian Granica, Jakub Hadzik, Adam Matkowski

Subject: Biology And Life Sciences, Plant Sciences Keywords: proanthocyanidins; Polygoni cuspidati rhizoma; mass spectrometry; stilbenoids

Online: 26 February 2019 (13:03:16 CET)

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One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

Preprint ARTICLE | doi:10.20944/preprints202404.2007.v1

Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways

Fillipa Blasco Tavares Pereira Lopes, Daniela Schlatzer, Mengzhen Li, Serhan Yilmaz, Rihua Wang, Xin Qi, Marzieh Ayati, Mehmet Koyuturk, Mark R Chance

Subject: Biology And Life Sciences, Biophysics Keywords: Methionine Oxidation; MSox; ROS; Proteomics; 5XFAD; Alzheimer’s Disease, mass spectrometry

Online: 30 April 2024 (17:07:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0373.v1

Detection and Verification of a Key Intermediate in an Enantioselective Peptide Catalyzed Acylation Reaction

Matthias Brauser, Tim Heymann, Christina Marie Thiele

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: acylation; imidazolium ion; mass spectrometry; NMR spectroscopy; organocatalysis

Online: 22 August 2022 (04:10:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1668.v1

Comparison of the Proteome of Staphylococcus aureus Planktonic Culture and 3-Day Biofilm Reveals Potential Role of Key Proteins in Biofilm

Md Arifur Rahman, Ardeshir Amirkhani, Durdana Chowdhury, Karen Vickery, Honghua Hu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Staphylococcus aureus, biofilms, proteome, TMT, mass spectrometry, virulence factors, biosynthetic processes, stress responses.

Online: 27 May 2024 (09:34:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1367.v1

Systematic Characterisation of the Fragmentation of Flavonoids using High-Resolution Accurate Mass Electrospray Tandem Mass Spectrometry

Candy Jiang, Paul Jonathan Gates

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: flavonoids; mass spectrometry; fragmentation; structure characterisation

Online: 17 October 2024 (10:11:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0493.v1

Observations from the proteomics bench

Simone König, Karin Schork, Martin Eisenacher

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mass spectrometry; bioinformatics; proteomics; false-positives

Online: 5 January 2024 (12:07:01 CET)

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Preprint REVIEW | doi:10.20944/preprints202304.0312.v1

Advanced Proteomic and Bioinformatic Tools for Predictive Analysis of Allergens in Novel Foods

María Dolores Pedrouso, José M. Lorenzo, Juan de Dios Alché, Ramón Moreira, Daniel Franco

Subject: Biology And Life Sciences, Food Science And Technology Keywords: novel proteins; food safety; allergenicity; mass spectrometry, omic technologies

Online: 13 April 2023 (08:30:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0312.v1

Rapid Assessment of Lipidomics Sample Purity and Quantity Using Fourier-Transform Infrared Spectroscopy

Harley Robinson, Jeffrey Molendijk, Alok K. Shah, Tony Rahman, Gregory J. Anderson, Michelle M. Hill

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: lipids; phospholipids; sphingolipids; triglycerides; FTIR; mass spectrometry; chemical contaminants

Online: 21 July 2022 (08:03:24 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202112.0300.v1

Current and Future Methodology for Quantitation and Site-specific Mapping the Location of DNA Adducts

Gunnar Boysen, Intawat Nookaew

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: DNA adducts; nanopore; Oxford Nanopore Technology; mass spectrometry; adductomics; exposome

Online: 20 December 2021 (09:53:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202410.0998.v1

Antimicrobial Profiling of Piper betle L. and Piper nigrum L. Against Methicillin-Resistant Staphylococcus aureus (MRSA): Integrative Analysis of Bioactive Compounds Based on FT-IR, GC-MS, and Molecular Docking Studies

Budiman Yasir, Suwahyuni Mus, Sitti Rahimah, Rein Mostatian Tandiongan, Kasandra Putri Klara, Nurul Afrida, Nur Rezky Khairun Nisaa, Risna Risna, Agum Wahyudha Jur, Gemini Alam, Abdul Rohman

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Piper extracts; cluster analysis; MRSA; fourier transform infrared; gas chromatography-mass spectrometry; In Silico Studies

Online: 15 October 2024 (03:47:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0362.v1

Threshold of Reactivity and Tolerance to Precautionary Allergen-Labelled Biscuits of Baked Milk- And Egg-Allergic Children

Vincenzo Fierro, Valeria Marzano, Linda Monaci, Pamela Vernocchi, Maurizio Mennini, Rocco Valluzzi, Stefano Levi Mortera, Rosa Pilolli, Lamia Dahdah, Veronica Calandrelli, Giorgia Bracaglia, Stefania Arasi, Carla Riccardi, Alessandro Fiocchi, Lorenza Putignani

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Labelling; Food Allergy; Prevention; Proteomics; Mass Spectrometry; Cow’s Milk Allergy

Online: 19 November 2021 (14:44:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.0606.v1

Profiling of Organosulfur Compounds in Onions: A Comparative Study between LC-HRMS and DTD-GC-MS

Ana V. González-de-Peredo, Alicia Maroto, Gerardo F. Barbero, Antony Memboeuf

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Allium cepa L.; cysteine sulfoxides; gas chromatography; high-resolution mass spectrometry; onion; organosulfur compounds

Online: 11 June 2024 (08:10:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0472.v1

Exploring the Western Shoreline of the Nuclear Chart: What Precision Mass Spectrometry with TITAN-TRIUMF Can Teach Us About Nuclear Existence

Annabelle Czihaly, Soenke Beck, Julian Bergmann, Callum L. Brown, Thomas Brunner, Timo Dickel, Jens Dilling, Eleanor Dunling, Jake Flowerdew, Zach Hockenbery, Andrew Jacobs, Brian Kootte, Stephan Malbrunot-Ettenauer, Fernando Maldonado Millán, Ali Mollaebrahimi, Erich Leistenschneider, Eleni Marina Lykiardopoulou, Ish Mukul, Stefan F. Paul, Wolfgang R. Plaß, Moritz Pascal Reiter, Christoph Scheidenberger, James L. Tracy, Jr., A. A. Kwiatkowski

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: Mass spectrometry; proton dripline; time-of-flight mass spectrometry; ion traps; radioactive ion beams; experimental nuclear physics

Online: 5 September 2024 (14:58:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1589.v1

Synthesis and Characterization of Recombinant Gelsolin 1-3 for Use in the Detection of Lysophosphatidic Acid, a Possible Ovarian Cancer Biomarker

Navina Lotay, Cynthia Maria Suarez, Brian De La Franier, Rebecca Ann Jockusch, Michael Thompson

Subject: Biology And Life Sciences, Life Sciences Keywords: gelsolin; SDS-PAGE; FTICR-MS; mass spectrometry; protein characterization

Online: 19 July 2024 (10:52:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0492.v1

Clinical Evaluation of VITEK MS PRIME with PICKME Pen for Bacteria and Yeasts, and RUO database for Filamentous Fungi

Hyeyoung Lee, Jehyun Koo, Junsang Oh, Sung-Il Cho, Hyunjoo Lee, Hyun Ji Lee, Gi-Ho Sung, Jayoung Kim

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: MALDI-TOF mass spectrometry; VITEK MS PRIME; PICKME; filamentous fungi; yeast; bacteria

Online: 8 April 2024 (08:19:41 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202403.0296.v1

Application of Magnetic Materials Combined with Echo® MS System in Ultra‐Fast and Ultra‐Sensitive Detection of Illegal Drugs in Sewage

Feiyu Yang, Kaijun Ma, Yichao Cao, Zhiyuan Li

Subject: Environmental And Earth Sciences, Other Keywords: detection technology of drugs in urban sewage; magnetic solid phase extraction; acoustic ejection mass spectrometry analysis method; fast detection

Online: 6 March 2024 (11:08:13 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.0726.v1

Mass Spectrometry in Clinical Chemistry: Advancing Biomarker Discovery and Personalized Medicine

Ahrum Son, Woojin Kim, Jongham Park, Yongho Park, Wonseok Lee, Sangwoon Lee, Hyunsoo Kim

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Liquid Chromatography-Mass Spectrometry; Clinical Chemistry; Biomarker Discovery; Personalized Medicine

Online: 12 August 2024 (03:50:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0268.v1

Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

Pascal Mülner, Elisa Schwarz, Kristin Dietel, Stefanie Herfort, Jennifer Jähne, Peter Lasch, Tomislav Cernava, Gabriele Berg, Joachim Vater

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: P. polymyxa; bioactive peptides; volatiles; MALDI-TOF mass spectrometry; GC-MS

Online: 19 October 2021 (10:29:01 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202401.2118.v1

Brimonidine Eye Drops at the Children Reach: A Possible Foe

Daniela Trotta, Mirco Zucchelli, Carmela Salladini, Patrizia Ballerini, Claudia Rossi, Maurizio Aricò

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Brimonidine, eye-drop poisoning, tandem mass spectrometry

Online: 30 January 2024 (12:44:49 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.0192.v1

Recent Progress in Mass Spectrometry-based Metabolomics in Major Depressive Disorder Research

Mingxia Liu, Wen Ma, Yi He, Zuoli Sun, Jian Yang

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: major depressive disorder; mass spectrometry; metabolomics; biomarkers

Online: 4 October 2023 (11:46:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0466.v1

Expanding the Analytical Toolbox: Developing New Peptide Map Methods to Fully Characterize the Proteolysis-resistant Single-domain Antibody New Modality

Y Diana Liu, Michelle Irwin Beardsley, Feng Yang

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Biopharmaceuticals; drug substances; sample preparation; mass spectrometry

Online: 7 August 2023 (10:01:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0442.v1

Photochemical Degradation of the UV Filter Octyl Methoxy Cinnamate Probed via Laser Interfaced Mass Spectrometry

Natalie G. K. Wong, Maria Sereli, Cate S Anstöter, Caroline E. H. Dessent

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: Sunscreens; Photodynamics; Cinnamates; Mass Spectrometry; Lasers; Photodegradation

Online: 23 November 2022 (08:31:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202101.0530.v1

Discovery of Simple Blood-Based Test to Predict Early Onset of Alzheimer’s Using Standard Clinical Mass Spectrometry Platforms

Simona Piccirella, Simone Cristoni, Christopher Fowler, James Doecke, Giulia Abate, Daniela Uberti

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer’s disease; biomarker; prognosis; P53; mass spectrometry

Online: 26 January 2021 (10:01:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints202006.0111.v1

A Novel Family of Quadrupole-Orbitrap Mass Spectrometers for a Broad Range of Analytical Applications

Jan-Peter Hauschild, Amelia Corinne Peterson, Erik Couzijn, Eduard Denisov, Denis Chernyshev, Christian Hock, Hamish Stewart, Ralf Hartmer, Dmitry Grinfeld, Christian Thoeing, Bastian Reitemeier, Arne Kreutzmann, Oliver Lange, Wilko Balschun, Aivaras Venckus, Sebastian Kanngiesser, Alexander Kholomeev, Gregor Quiring, Frank Czemper, Eloy Wouters, Michael Belford, Mathias Mueller, Jens Grote, Tabiwang N. Arrey, Julia Kraegenbring, Kerstin Strupat, Siegrun Mohring, Catharina Crone, Alexander Harder, Aaron Robitaille, Aaron Gajadhar, Khatereh Motamed, Yang Liu, Daniel Lopez Ferrer, Nicolaie Eugen Damoc, Angela Criscuolo, Kristina Srzentic, Ed George, Charles Yang, Ioanna Ntai, Amanda Souza, Andreas Wieghaus, Alexander Makarov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Orbitrap mass spectrometry; high resolution; accurate mass

Online: 8 June 2020 (10:17:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202405.2130.v1

Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools – Chances and Limitations. A Critical Review

Joachim Müller, Ghalia Boubaker, Norbert Müller, Anne-Christine Uldry, Sophie Braga-Lagache, Manfred Heller, Andrew Hemphill

Subject: Biology And Life Sciences, Parasitology Keywords: Adaptation; mass spectrometry; model system; mode of action; resistance; target

Online: 31 May 2024 (10:36:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0281.v1

The Experimental Proteome of Leishmania infantum promastigote and Its Usefulness for Improving Gene Annotations

África Sanchiz, Esperanza Morato, Alberto Rastrojo, Esther Camacho, Sandra Gonzalez-De la Fuente, Anabel Marina, Begoña Aguado, Jose M. Requena

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Leishmania infantum; proteome; post-translational modifications (PTMs); proteogenomics; mass spectrometry

Online: 12 August 2020 (10:10:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0922.v1

Design and Implementation of an Orbitrap Mass Spectrometer Data Acquisition System for Atmospheric Molecule Identification

Wei Wang, Yongping Li

Subject: Engineering, Electrical And Electronic Engineering Keywords: data acquisition; Orbitrap mass spectrometry; spectrum analysis; FPGA; FFT analysis

Online: 25 April 2023 (10:29:05 CEST)

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Orbitrap mass spectrometers have been widely used in environmental component analysis . This paper presents an enhanced data acquisition system for in-situ detection of Orbitrap mass spectrometry, which enables in-suit real-time signal processing and analysis for atmospheric molecules with small mass numbers.During previous space atmospheric explorations, quadrupole mass spectrometry (QMS) was usually utilized for analyzing isotopic compositions and complicated compounds in the mixture. However, the inevitable ion scattering and drift during mass transfer, as well as the QMS attenuator loss, result in a relatively lower signal-to-noise ratio of the produced mass spectrum. In recent years, a novel mass spectrometer, called an Orbitrap mass spectrometer, has been gradually accepted and used because of its non-destructive detection ability, high mass resolution, and accuracy. Nevertheless, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) is preferred over the Orbitrap mass spectrometry for ground applications, especially for detecting low-mass components (e.g. below 50 atomic mass units) due to the former’s superior resolution. As the mass number decreases, the detection system demands a higher sampling and processing rate. Additionally, in ground-based scenarios, mass spectrometry analysis software on computers is often employed to analyze the signals. Therefore, in order to achieve in-situ detection of the space atmospheric environment using the Orbitrap mass spectrometer, this paper proposes a real-time signal acquisition and processing system for mass spectrometry analysis. The system comprises of signal conditioning circuits, analog-to-digital conversion circuits, programmable logic circuits, and related software. These components perform spectrum analysis and process the signal in real-time on hardware components, allowing for high-speed acquisition and analysis of the signals produced by the Orbitrap mass spectrometer.

Preprint ARTICLE | doi:10.20944/preprints202307.0350.v1

Distribution and Chemistry of Phoenixin-14, a Newly Discovered Sensory Transmission Molecule in Porcine Afferent Neurons

Urszula Mazur, Ewa Lepiarczyk, Paweł Janikiewicz, Elżbieta Łopieńska-Biernat, Mariusz Krzysztof Majewski, Agnieszka Bossowska

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: phoenixin-14; pig; dorsal root ganglia; neuropeptides; immunofluorescence; mass spectrometry

Online: 5 July 2023 (16:14:39 CEST)

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Phoenixin-14 (PNX) - a bioactive peptide recently discovered in the rat brain, is highly conserved among many animal species: rodents (rat, mouse), pig, dog as well as humans. In rodents, PNX is expressed in areas responsible for the transmission of sensory information dorsal root ganglia (DRG) neurons, where it is present at relatively high levels and may preferentially suppress visceral pain. However, so far the presence of PNX has not been investigated in DRG in pigs, a species which, due to its anatomical and histological similarity to humans, is considered a better model for biomedical studies than rodents. The present study aimed to investigate the immunoreactivity of PNX in the DRG of the domestic pig. The collected spinal ganglia from the cervical (C), thoracic (Th), lumbar (L) and sacral (S) sections were transversely divided into serial sections of 10 μm thickness. DRG sections from each level of the spinal cord were double-labeled immunohistochemically using antibodies to PNX in a mixture with antibodies to: cocaine and amphetamine related transcript (CART), calretinin (CRT), calcytonin gene-related peptide (CGRP), galanin (GAL), nitric oxide synthase (nNOS), pituitary adenylate cyclase-acrivating polypeptide (PACAP), substance P (SP) or somatostatin (SOM), respectively. Immunohistochemical studies revealed PNX immunoreactivity in approximately 20% of nerve cells in all DRG examined, highlighting mainly the presence of the peptide in cells of small diameter (approximately 74% of all PNX-positive neurons found). Double labeling of DRG sections showed that PNX-immunopositive neurons stained also for CGRP (96.1%), SP (88.5%), nNOS (52.1%), GAL (20.7%), CRT (10.05%), PACAP (7.4%), CART (5.1%), or SOM (4.7%). Our research revealed for the first time the presence of the new peptide PNX in the sensory ganglia of the domestic pig, its co-localization with other important neurotransmitters involved in sensory transmission and its percentage distribution in ganglion domains. The exact function of PNX in DRG is not yet known, however, the high degree of co-localization of this peptide with the main nociceptive transmitters SP and CGRP may indicate its function in modulation of pain transmission.

Preprint ARTICLE | doi:10.20944/preprints201712.0082.v1

Chromatographic and Spectroscopic Identification and Recognition of Natural Dyes, Uncommon Dyestuff Components and Mordants Found in the 16th Century Carpet

Olga Otłowska, Marek Ślebioda, Agata Kot-Wasik, Jakub Karczewski, Magdalena Śliwka-Kaszyńska

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: natural dyes; flavonoids; flavone glycosides; anthraquinones; extraction procedure; liquid chromatography mass spectrometry

Online: 13 December 2017 (17:58:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0456.v1

Composition of the Scent in Some Ophrys Orchids Growing in Basilicata (Southern Italy). A Solid Phase Microextration Study Coupled with Gas Chromatography and Mass Spectrometry

Maurizio D’Auria, Richard Lorenz, Marisabel Mecca, Rocco Racioppi, Vito Antonio Romano

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: Ophrys; scent composition; solid phase microextraction; gas chromatography mass spectrometry

Online: 9 October 2023 (08:55:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0484.v1

Dynamics of Cyanobacterial Global Metabolom in Response to Light and Temperature Variations

Damien Le Moigne, Justine Demay, Anita Reinhardt, Cécile Bernard, Sandra Kim Tiam, Benjamin Marie

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: cyanobacteria; metabolomics; high-resolution mass spectrometry; secondary metabolite induction; culture conditions

Online: 25 August 2021 (10:48:20 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202408.0457.v1

Performance of the VITEK MS System for the Identification of Filamentous Fungi in a Routine Microbiological Laboratory in Chile

Lorena Porte, Rodrigo Cruz, Inia Pérez, Carmen Varela, Cristina Díaz, Patricia García, Paulette Legarraga, Francisca Valdivieso, Thomas Weitzel

Subject: Medicine And Pharmacology, Other Keywords: Diagnosis; MALDI-TOF; fungal infections; mycology; mass spectrometry

Online: 8 August 2024 (04:18:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0270.v1

The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection

Natalie Iannuzo, Alane Blythe C. Dy, Stefano Guerra, Paul R. Langlais, Julie G. Ledford

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Mycoplasma pneumoniae; CC16; CCSP; CC10; mass spectrometry; remodeling

Online: 5 June 2023 (08:28:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0574.v2

Mass Spectrometry-Based Techniques Towards OMICS 2.0

Ivan Šoša

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: cost; mass spectrometry; OMICS; OMICS 2.0: Society 5.0

Online: 27 January 2023 (06:59:30 CET)

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Preprint REVIEW | doi:10.20944/preprints201808.0410.v1

High-Throughput Direct Mass Spectrometry-Based Metabolomics to Characterize Metabolite Fingerprints Associated with Alzheimer’s Disease Pathogenesis

Raúl González-Domínguez, Ana Sayago, Ángeles Fernández-Recamales

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: metabolomics; direct mass spectrometry; Alzheimer’s disease; pathogenesis; biomarkers

Online: 23 August 2018 (10:10:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0493.v1

Direct in vivo Analysis of CBD- and THC-Acid Type Cannabinoids and Classification of Cannabis Cultivars by SpiderMass

Nina Ogrinc, Serge Schneider, Adèle Bourmaud, Michel Salzet, Isabelle Fournier

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: water-assisted laser desorption/ionization; SpiderMass; cannabinoids; mass spectrometry; plants; in vivo

Online: 30 December 2021 (19:02:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202410.1911.v1

Antioxidant Peptides from Sacha Inchi Meal: An In Vitro, Ex Vivo, and In Silico Approach

Erwin Torres-Sánchez, Iván Lorca-Alonso, Sandra González-de la Fuente, Blanca Hernández-Ledesma, Luis-Felipe Gutiérrez

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Plukenetia volubilis; INFOGEST; mass spectrometry; bioactive peptides; bioinformatics; resistant peptides; bioavailability

Online: 24 October 2024 (11:59:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2264.v1

From Organotypic Mouse Brain Slices to Human Alzheimer’s Plasma Biomarkers: A Focus on Nerve Fiber Outgrowth

Sakir Necat YILMAZ, Katharina Steiner, Josef Marksteiner, Klaus Faserl, Mathias Villunger, Bettina Sarg, Christian Humpel

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer’ s disease; biomarker; plasma; organotypic brain slice; nerve fiber; myelin oligodendrocyte glycoprotein; microcontact printing; mass spectrometry

Online: 30 September 2024 (13:07:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0553.v1

Analysis of Volatile Compounds and Flavor Fingerprint Using Gas Chromatography–Ion Mobility Spectrometry (GC–IMS) on Crassostrea gigas With Different Ploidy and Gender

Jingjing Fu, YouMei Sun, Mingxian Cui, Enshuo Zhang, Luyao Dong, Yanchun Wang, Weijun Wang, Zan Li, Jianmin Yang

Subject: Biology And Life Sciences, Aquatic Science Keywords: Crassostrea gigas; Gas chromatography–ion mobility spectrometry (GC–IMS); Ploidy; Volatile; Flavor

Online: 9 May 2023 (03:48:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0258.v1

Rapid Monitoring of Organochlorine Pesticides Residues in Various Fruit Juices and Water Samples Using Fabric Phase Sorptive Extraction and Gas Chromatography-Mass Spectrometry

Ramandeep Kaur, Ripneel Kaur, Susheela Rani, Ashok Kumar Malik, Abuzar Kabir, Kenneth G. Furton, Victoria Samanidou

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Fabric phase sorptive extraction; Gas chromatography-Mass Spectrometry; Organochlorine pesticides; Sample preparation

Online: 25 January 2019 (14:56:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1985.v2

The Exometabolome of Xylella fastidiosa in Contact with Paraburkholderia phytofirmans Supernatant Reveals Changes in Nicotinamide, Amino Acids, Biotin, and Plant Hormones

Oseias R. Feitosa-Junior, Andrea Lubbe, Suzanne M. Kosina, Joaquim Martins-Junior, Deibs Barbosa, Clelia Baccari, Paulo A. Zaini, Benjamin P. Bowen, Trent R. Northen, Steven E. Lindow, Aline Maria Da Silva

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Xylella fastidiosa; Paraburkholderia phytofirmans; metabolomics; phytopathogen; liquid chromatography mass-spectrometry; MAGI

Online: 7 December 2023 (09:16:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0373.v1

The Hidden Story on Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma – Association with Clinical Outcome and Tumor Phenotypes

Lazaro Hiram Betancourt, A. Marcell Szasz, Magdalena Kuras, Jimmy Rodriguez Murillo, Yutaka Sugihara, Indira Pla, Zsolt Horvath, Krzysztof Pawlowski, Melinda Rezeli, Kenichi Miharada, Jeovanis Gil, Jonatan Eriksson, Roger Appelqvist, Tasso Militois, Bo Baldetorp, Christian Ingvar, Håkan Olsson, Lotta Lundgren, Peter Horvatovich, Charlotte Welinder, Elisabet Wieslander, Ho Jeong Kwon, Johan Malm, Istvan Balazs Nemeth, Göran Jönsson, David Fenyo, Aniel Sanchez, György Marko-Varga

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: malignant melanoma; BRAF V600E mutation; proteomics; mass spectrometry genetics; heterogeneity; prognosis

Online: 31 October 2019 (10:36:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints201902.0016.v1

Simultaneous Determination of Pharmaceuticals by Solid-phase Extraction and Liquid Chromatography–tandem Mass Spectrometry: A Case Study from Sharjah Sewage Treatment Plant

Mohammad H. Semreen, Abdallah Shanableh, Lucy Semerjian, Hasan Alniss, Muath Mousa, Xuelian Bai, Kumud Acharya

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: liquid chromatography–tandem mass spectrometry; pharmaceuticals; wastewater analysis; solid phase extraction

Online: 2 February 2019 (03:21:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.2105.v1

Energy-Resolved Mass Spectrometry and Mid-Infrared Microscopy for Purity Assessment of a Synthetic Peptide Cyclised by Intramolecular Click Chemistry

Alicia Maroto, Ricard Boqué, Dany Jeanne dit Fouque, Antony Memboeuf

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: peptide isomers; quantification; click chemistry; energy-resolved mass spectrometry; infrared microscopy; multivariate and univariate calibration; cyclic peptide

Online: 26 September 2024 (14:49:57 CEST)

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Cyclic peptides have higher stability and better properties as therapeutic agents than their linear peptide analogues. Consequently, intramolecular click chemistry is becoming an increasingly popular method for the synthesis of cyclic peptides from their isomeric linear peptides. However, assessing the purity of these cyclic peptides by mass spectrometry is a significant challenge, as the linear and cyclic peptides have identical masses. In this paper, we have evaluated the analytical capabilities of energy-resolved mass spectrometry (ER MS) and mid-infrared microscopy (IR) to address this challenge. On the one hand, mixtures of both peptides were subjected to Collision Induced Dissociation tandem mass spectrometry (CID MS/MS) experiments in an ion trap mass spectrometer at several excitation energies. Two different calibration models were used: a univariate model (at a single excitation voltage) and a multivariate model (using multiple excitation voltages). The multivariate model demonstrated slightly enhanced analytical performance, which can be attributed to more effective signal averaging when multiple excitation voltages are considered. On the other hand, IR microscopy was used for the quantification of the relative amount of linear peptide. This was achieved through univariate calibration, based on the absorbance of an alkyne band specific to the linear peptide, and through Partial Least Squares (PLS) multivariate calibration. The PLS calibration model demonstrated superior performance in comparison to univariate calibration, indicating that consideration of the full IR spectrum is preferable to focusing on the specific peak of the linear peptide. The advantage of IR microscopy is that it is linear across the entire working interval, from linear peptide molar ratios of 0 (equivalent to pure cyclic peptide) up to 1 (pure linear peptide). In contrast, the ER MS calibration models exhibited linearity only up to 0.3 linear peptide molar ratio. However, ER MS showed better performances in terms of limit of detection, intermediate precision and root-mean-square-error of calibration. Therefore, ER MS is the optimal choice for the detection and quantification of the lowest relative amounts of linear peptide.

Preprint REVIEW | doi:10.20944/preprints202007.0523.v1

Proteomics-based Methodologies for the Detection and Quantification of Seafood Allergens

Mónica Carrera, Manuel Pazos, María Gasset

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: discovery proteomics; targeted proteomics; mass spectrometry; fish allergens; crustacean allergens; mollusk allergens

Online: 22 July 2020 (11:23:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1628.v1

High Mitochondrial Protein Expression as a Potential Predictor of Relapse Risk in Acute Myeloid Leukemia Patients with the Monocytic FAB subtypes M4 and M5

Frode Selheim, Elise Aasebø, Øystein Bruserud, Maria Hernandez-Valladares

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: acute myeloid leukemia; mass spectrometry; proteomic; phosphoproteomic; relapse; mitochondria; FAB subtypes

Online: 25 October 2023 (10:07:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1683.v1

COVID-19 Infection during Pregnancy: Disruptions in Lipid Metabolism and Implications for Newborn Health

Natalya A. Frankevich, Alila Tokareva, Vitaliy V. Chagovets, Natalia Starodubtseva, Natalia V. Dolgushina, Roman G. Shmakov, Gennady T. Sukhikh, Vladimir E. Frankevich

Subject: Biology And Life Sciences, Life Sciences Keywords: SARS-CoV-2; COVID-19; pregnancy; plasma; metabolomics; lipidomics; mass spectrometry; biomarkers

Online: 26 July 2023 (02:33:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0145.v1

Compounds Characterization of a Mucuna Seed Extract: L-Dopa, Arginine, Stizolamine, and Some Fructooligosaccharides

Ana Lilia Hernández-Orihuela, Karla Viridiana Castro-Cerritos, Mercedes Guadalupe López, Agustino Martínez-Antonio

Subject: Biology And Life Sciences, Plant Sciences Keywords: Mucuna seed; L-Dopa content; Lyophilized extract; Sugars identification; TLC; Mass spectrometry

Online: 8 November 2022 (03:31:47 CET)

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Preprint REVIEW | doi:10.20944/preprints202107.0193.v1

Plants Metabolome Study: Emerging Tools and Techniques

Manish Kumar Patel, Sonika Pandey, Manoj Kumar, Intesaful Haque, Sikander Pal, Narendra Singh Yadav

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: metabolomics; plant biology; metabolomics databases; data analysis; metabolomics software tools; mass spectrometry; omics

Online: 8 July 2021 (10:46:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201911.0145.v1

Aerosol from Biomass Combustion in Northern Europe: Influence of Meteorological Conditions and Air Mass History

Jun Noda, Robert Bergström, Xiangrui Kong, Torbjörn L. Gustafsson, Borka Kovacevik, Maria Svane, Jan B. C. Pettersson

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: biomass burning; residential wood combustion; aerosol mass spectrometry; potassium; chemical transport model

Online: 13 November 2019 (11:34:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0750.v1

Detecting Intermediates and Products of Fast Heterogeneous Reactions on Liquid Surfaces via Online Mass Spectrometry

Agustín J. Colussi, Shinichi Enami

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: fast gas-liquid reactions; online electrospray ionization mass spectrometry; heterogeneous atmospheric chemistry

Online: 31 October 2018 (10:54:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1194.v1

Rapid Determination of Taraxacum kok-saghyz Rubber Content by Pyrolyzer Hyphenated with a Miniaturized Mass Spectrometer

Shunkai Gao, Minmin Guo, Jiaqi Gao, Zejian Huang, Min Gan, Jichuan Zhang, Yiyang Dong

Subject: Biology And Life Sciences, Plant Sciences Keywords: Taraxacum kok-saghyz; natural rubber; quantitative; yrolysis-mass spectrometry

Online: 16 January 2024 (06:53:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0462.v1

Cisplatin Dependent Secretion of Immunomodulatory High Mobility Group Box 1 (HMGB1) Protein from Lung Cancer Cells

Kevin Gillespie, Ross Pirnie, Clementina Mesaros, Ian Blair

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Platinum drugs; acetylation; oxidation; immune response; mass spectrometry; chromatography

Online: 7 August 2023 (10:10:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0975.v1

N-Glycomic Profiling of Microsatellite Unstable Colorectal Cancer

Iiris Ukkola, Pirjo Nummela, Annamari Heiskanen, Matilda Holm, Sadia Zafar, Mia Kero, Caj Haglund, Tero Satomaa, Soili Kytölä, Ari Ristimäki

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: BRAFV600E; colorectal cancer; microsatellite instability; mass spectrometry; N-glycosylation

Online: 14 June 2023 (04:02:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0304.v2

Endogenous TOM20 Proximity Labelling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells

Sébastien Meurant, Lorris Mauclet, Marc Dieu, Thierry Arnould, Sven Eyckerman, Patricia Renard

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Mitochondria; Co-translational import; BioID; Protein identification; Mass spectrometry

Online: 21 April 2023 (09:03:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0308.v1

Characterization Techniques Coupled With Mathematical Tools for Deepening Asphaltenes Structure

Barbara Apicella, Anna Ciajolo, Andrea Carpentieri, Ciprian Popa, Carmela Russo

Subject: Engineering, Energy And Fuel Technology Keywords: Asphaltenes; mass spectrometry; DBE; Size exclusion chromatography; XRD; Aliphaticity

Online: 17 November 2021 (12:54:15 CET)

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Working Paper REVIEW

Evaluation of the Sensitivity of Proteomics Methods Using the Absolute Copy Number of Proteins in a Single Cell as a Metric

Benjamin Orsburn

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Proteomics, protein informatics, mass spectrometry, single cell, copy number

Online: 5 July 2021 (16:09:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0324.v1

Alteration in the cerebrospinal fluid lipidome in Parkinson´s disease: A pilot study

Joaquín Fernández-Irigoyen, Paz Cartas-Cejudo, Enrique Santamaría

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: lipids; cerebrospinal fluid, parkinson´s disease, mass-spectrometry, lipidomics

Online: 12 April 2021 (21:16:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202103.0289.v1

Sphingolipidome Quantification by Liquid Chromatography- High Resolution Mass Spectrometry: Whole Blood vs. Plasma

Dezhen Wang, Peining Xu, Clementina Mesaros

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: sphingolipidome; ceramides; high resolution mass spectrometry; whole blood; plasma

Online: 10 March 2021 (16:06:08 CET)

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Working Paper ARTICLE

Recycling of Waste Cooking Oils: Variation of the Chemical Composition during Water Treatment

Alberto Mannu, Monica Ferro, Greta Colombo Dugoni, Walter Panzeri, Giacomo Luigi Petretto, Paolo Urgeghe, Andrea Mele

Subject: Chemistry And Materials Science, Food Chemistry Keywords: waste cooking oil; volatiles; 1H NMR; mass spectrometry; prototype

Online: 16 April 2019 (11:19:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201808.0401.v1

Polycyclic Aromatic Hydrocarbons in Soil Samples from Diboll, East Texas (USA)

Kefa Karimu Onchoke, Jorge J. Ojeda, Michael A. Janusa

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: soil; PAHs; diboll; fluoranthene; pyrene; gas chromatography-mass spectrometry

Online: 23 August 2018 (04:18:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0219.v1

Mass Spectrometry Rearrangement Ions and Metabolic Pathways-Based Discovery of Indole Derivatives during the Aging Process in Citrus reticulata ‘Chachi’

Tian Li, Ke Chen, Xiaoming Wang, Ying Wang, Yue Su, Yinlong Guo

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Citrus reticulata 'Chachi'; methyl N-methylanthranilate; mass spectrometry; 3-hydroxyindole; indole derivatives

Online: 3 November 2023 (05:18:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0785.v1

Investigation on the Integration of Low-Cost NIR Spectrometers in Mill Flour Industries for Protein, Moisture and Ash Content Estimation

Vasileios Boglou, Dimosthenis Verginadis, Athanasios Karlis

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Nondestructive Instrumentation; Near-Infrared Spectrometry; parameter estimation; optimization algorithm; Fuzzy-Cognitive-Maps

Online: 15 September 2023 (03:49:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202303.0241.v1

Analysis of Nipah Virus Replication and Host Proteome Response Patterns in Differentiated Porcine Airway Epithelial Cells Cultured at the Air-Liquid Interface

Martin Müller, Kerstin Fischer, Elisabeth Woehnke, Luca M. Zaeck, Christoph Prönnecke, Michael R. Knittler, Axel Karger, Sandra Diederich, Stefan Finke

Subject: Biology And Life Sciences, Virology Keywords: Nipah virus; air-liquid interface culture; respiratory epithelium; mass spectrometry; immune response

Online: 14 March 2023 (02:50:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202107.0288.v1

Narrow Precursor Mass Range for DIA-MS Enhances Protein Identification and Quantification

Huoming Zhang, Dalila Bensaddek

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: data-independent acquisition (DIA); mass spectrometry, precursor mass range selection, Arabidopsis; quantitative proteomics.

Online: 13 July 2021 (10:06:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0174.v1

Aroma Identification and Classification in 18 Kinds of Teas (Camellia sinensis) by Sensory Evaluation, Hs-Spme-Gc-Ims/Gc×gc-Ms, and Chemometrics

Yanping Lin, Ping Yang, Ying Wang, Yibiao Huang, Huanlu Song

Subject: Biology And Life Sciences, Food Science And Technology Keywords: tea (Camellia sinensis); solid phase micro extraction (SPME); two-dimensional comprehensive gas chromatography-olfactometry-mass spectrometry analysis (GC × GC-O-MS); gas chromatog-raphy-ion mobility spectrometry (GC-IMS); aroma compounds

Online: 2 June 2023 (09:59:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0040.v1

Advanced Microsamples: Current Applications and Considerations for Mass Spectrometry-Based Metabolic Phenotyping Pipelines

Jayden Lee Roberts, Luke Whiley, Nicola Gray, Melvin Gay, Nathan G. Lawler

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Microsampling; sample miniaturisation; dried blood spot (DBS); dried plasma spot (DPS); dried serum spot (DSS); metabolic phenotyping; gas chromatography-mass spectrometry (GC-MS); liquid chromatog-raphy-mass spectrometry (LC-MS)

Online: 3 June 2022 (09:53:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0630.v1

Validation of A MS Based Proteomics Method for Milk and Egg Quantification in Cookies at The Lowest VITAL Levels: An Alternative to The Use of Precautionary Labeling

Linda Monaci, Elisabetta De Angelis, Rocco Guagnano, Aristide P. Ganci, Iganzio Garaguso, Alessandro Fiocchi, Rosa Pilolli

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: egg; milk; allergens; multiple reaction monitoring; mass spectrometry; reference doses; food; PAL

Online: 28 August 2020 (09:40:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0100.v1

Modulatory Effect of Polyphenolic Compounds from the Mangrove tree Rhizophora mangle L. Improving Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice

Leonardo Mendes de Souza Mesquita, Cíntia Rabelo e Paiva Caria, Paola Souza Santos, Caio Cesar Ruy, Natalia da Silva Lima, Débora Kono Taketa Moreira, Claudia Quintino da Rocha, Daniella Carisa Murador, Veridiana Vera de Rosso, Alessandra Gambero, Wagner Vilegas

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: Cd36; mass spectrometry; NAFLD; anti-lypase activity; anti-amylase activity; insulin resistance

Online: 9 April 2018 (08:28:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0886.v1

Tandem Mass Spectrometry Screening for Inborn Errors of Metabolism: Comprehensive Bibliometric Analysis

Victoria Kononets, Gulmira Zharmakhanova, Saule Balmagambetova, Lyazzat Syrlybayeva, Gulshara Berdesheva, Zhanna Zhussupova, Aidana Tautanova, Yergen Kurmambayev

Subject: Public Health And Healthcare, Primary Health Care Keywords: tandem mass spectrometry; inborn errors of metabolism; newborn screening; selective screening; scientometrics; bibliometric analysis; visualization; VOSviewer

Online: 16 February 2024 (05:37:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.1178.v1

Fabrication of a Microfluidic-Based Device Coated with Polyelec-Trolyte-Capped-Titanium Dioxide to Couple High-Performance Liquid Chromatography with Inductively Coupled Plasma-Mass Spectrometry for Mercury Speciation

Ji-Hao Chen, Yu-Ting Luo, Yi-An Su, Yan-Ren Ke, Ming-Jay Deng, Wei-Yu Chen, Cheng-Yu Wang, Jia-Lin Tsai, Cheng-Hsing Lin, Tsung-Ting Shih

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: mercury (Hg) speciation; microﬂuidic-based device; poly(methyl methacrylate) (PMMA); tita-nium dioxide nanoparticles (nano-TiO2); poly(diallyldimethylammonium chloride) (PDADMAC); inductively coupled plasma-mass spectrometry (ICP-MS)

Online: 15 July 2024 (12:30:19 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202112.0420.v3

Essential Terminology and Considerations for Validation of Non-targeted Methods

Kapil Nichani, Steffen Uhlig, Manfred Stoyke, Sabine Kemmlein, Franz Ulberth, Ilka Haase, Maik Döring, Stephan G. Walch, Petra Gowik

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Non-targeted methods; method validation; food fraud; food authenticity; mass spectrometry; spectroscopy; NGS; NMR

Online: 23 May 2022 (11:10:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.0548.v1

Advances in Mass Spectrometry of Gangliosides Expressed in Brain Cancers

Maria Roxana Biricioiu, Mirela Sarbu, Raluca Ica, Željka Vukelić, Svjetlana Kalanj-Bognar, Alina D. Zamfir

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: brain cancers; gangliosides; mass spectrometry; biomarker discovery; screening; structural analysis

Online: 7 December 2023 (19:55:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0105.v1

Thermal Diffusivity of Aqueous Dispersions of Silicon Oxide Nanoparticles by Dual-Beam Thermal-Lens Spectrometry

Vladislav R. Khabibullin, Liliya O. Usoltseva, Ivan V. Mikheev, Mikhail A. Proskurnin

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: thermal lens spectrometry; thermal diffusivity; dispersions; thermal effects; measurement sensitivity

Online: 6 March 2023 (14:00:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202105.0761.v1

Sexual Dimorphism in Crowned Lemur Scent-Marking.

Emily Elwell, David Walker, Stefano Vaglio

Subject: Social Sciences, Psychology Keywords: communication; olfaction; scent-marking; gas chromatography–mass spectrometry; Eulemur coronatus

Online: 31 May 2021 (12:09:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0743.v1

Applications of PERALS-Alpha Spectrometry for the Investigation of Radionuclides in Water Samples

Markus Raymond Zehringer, Franziska Kammerer, Anja Pregler

Subject: Physical Sciences, Acoustics Keywords: PERALS; alpha spectrometry; Polonium; Radon; Radium; Radiostrontium; Thorium; Uranium; Actinides

Online: 31 May 2021 (11:16:23 CEST)

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