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Preprint ARTICLE | doi:10.20944/preprints202105.0095.v1

The microRNA Landscape of Acute Beta Cell Destruction in Type 1 Diabetic Recipients of Intraportal Islet Grafts

Geert Antoine Martens, Geert Stange, Lorenzo Piemonti, Jasper Anckaert, Zhidong Ling, Daniel Pipeleers, Frans Gorus, Pieter Mestdagh, Dieter De Smet, Jo Vandesompele, Bart Keymeulen, Sarah Roels

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: beta cell, type 1 diabetes, islet transplantation, biomarkers, microRNA

Online: 6 May 2021 (15:01:45 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202012.0804.v1

A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene-Network during Host Interactions.

Carlos Pérez Arques, María Isabel Navarrro Mendoza, Laura Murcia, Marta Sanchis, Javier Capilla, Eusebio Navarro, Victoriano Garre, Francisco Esteban Nicolás Molina

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: white collar; mucormycosis; virulence; Mucorales; Mucor lusitanicus

Online: 31 December 2020 (12:46:05 CET)

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Mucolares are an ancient group of fungi encompassing the causal agents for the lethal infection mucormycosis. The high lethality rates, the emerging character of this disease, and the broad antifungal resistance of its causal agents are mucormycosis features alarming clinicians and researchers. Thus, the research field around mucormycosis is currently focused on finding specific weaknesses and targets in Mucorales for developing new treatments against mucormycosis. In this work, we tested the role of the white-collar genes family in the virulence potential of Mucor lusitanicus. In this regard, the study of the role in virulence of the three genes of this family, mcwc-1a, mcwc-1b, and mcwc-1c, resulted in a marked functional specialization, as only mcwc-1a was essential to maintain the virulence potential of M. lusitanicus. The traditional role of wc-1 genes regulating light-dependent responses is a thoroughly studied field, whereas their role in virulence remains uncharacterized. In this work, we investigated the mechanism involving mcwc-1a in virulence from an integrated transcriptomic and functional approach during the host-pathogen interaction. Our results revealed mcwc-1a as a master regulator controlling an extensive gene-network. Further dissection of this gene-network clustering its components by type of regulation and functional criteria disclosed a multifunctional mechanism depending on diverse pathways. In the absence of phagocytic cells, mcwc-1a controlled pathways related to cell motility and cytoskeleton that could be associated with the essential tropism during tissue invasion. After phagocytosis, several oxidative responding pathways dependent on mcwc-1a were activated during the germination of M. lusitanicus spore inside of phagocytic cells, which is the first stage of the infection. The third relevant group of genes involved in virulence and regulated by mcwc-1a belonged to the “unknown function,” indicating that new and hidden pathways are involved in virulence. The unknown function category is especially pertinent in the study of mucormycosis, as it is highly enriched in specific fungal genes that represent the most promising targets for developing new antifungal compounds. These results essentially unveil a complex multifunctional mechanism used by wc-1 genes to regulate the pathogenic potential in Mucorales that could also apply to other fungal pathogens.

Working Paper ARTICLE

Association of High Pre-Transplant Caveolin-1 Serum Concentrations with Attenuated Acute Cellular Rejection in Kidney Transplantation

Florian Emmerich, Stefan Zschiedrich, Christine Reichenbach-Braun, Caner Süsal, Susana Minguet, Marie-Christin Pauly, Maximilian Seidl

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Caveolin-1; kidney transplantation; graft rejection; ischemia; reperfusion injury (IRI)

Online: 1 December 2020 (14:08:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints201807.0477.v1

Improving Spinnability of Hyper-coal Derived Spinnable Pitch through the Hydrogenation with 1, 2, 3, 4-tetrahydroquinoline

Jianxiao Yang, Wei Wu, Xiaxiang Zhang, Kui Shi, Xuanke Li, Seong-Ho Yoon

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: hyper-coal, hydrogenation, spinnable pitch, carbon fiber

Online: 25 July 2018 (10:19:51 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1810.v1

Indoleamine 2,3-Dioxygenase 2 (IDO2): The Protein That Lived Twice

Chiara Suvieri, Maria Laura Belladonna, Claudia Volpi

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: indoleamine 2,3-dioxygenase 2 (IDO2); cancer; signaling function; tryptophan metabolism; indoleamine 2,3-dioxygenase 1 (IDO1); apo-enzyme

Online: 23 September 2024 (15:31:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1633.v1

Microcystis viridis NIES-102 Algae Lectin MVL Interacts with the COVID-19 Spike Protein RBDs via Protein-Protein Interaction

Zhengguang Wang, Zhihan Yang, Mami Shishido, Khadija Daoudi, Masafumi Hidaka, Hiroaki Tateno, Eugene Futai, Tomohisa Ogawa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Lectin; COVID-19; Algae; Carbohydrate-specificity; Protein-protein interaction

Online: 24 May 2024 (14:12:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1810.v1

Thermodynamic and Dynamic Transitions and Interaction Aspects in Reorientation Dynamics of Molecular Probe in Organic Compounds: A Series 1-Alkanols with TEMPO

Josef Bartoš, Helena Švajdlenková

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: 1-alkanols; spin probe TEMPO; ESR; thermodynamic transitions; viscosity; crossover transition; polarity; proticity

Online: 28 August 2023 (03:54:42 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202308.1253.v1

Retinal Microvascular Alterations in a Patient with Type 1 Diabetes, Hemoglobin D Hemoglobinopathy and High Myopia – Case Report and Review of Literature

Alexandra Oltea Dan, Andrei Teodor Bălășoiu, Ileana Puiu, Andreea Cornelia Tanasie, Anca Elena Târtea, Veronica Sfredel

Subject: Medicine And Pharmacology, Ophthalmology Keywords: OCT angiography, diabetes type 1, hemoglobin D, high myopia

Online: 17 August 2023 (05:31:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0772.v1

Plant-based beverages from germinated and ungerminated seeds, as a source of probiotics, and bioactive compounds with health benefits – Part 1: Legumes

Adriana Dalila Criste, Adriana Cristina Urcan, Cristian Ovidiu Coroian, Lucian Copolovici, Dana Maria Copolovici, Ramona Flavia Burtescu, Neli Kinga Oláh

Subject: Biology And Life Sciences, Food Science And Technology Keywords: plant-based beverages; lupine; chickpea; Lactobacillus plantarum; probiotic drinks; bioactive compounds.

Online: 11 May 2023 (02:56:52 CEST)

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Preprint REVIEW | doi:10.20944/preprints202106.0023.v1

On the Role of Paraoxonase-1, Chemokine (C-C motif) Ligand 2 and Metabolism in Oxidation, Inflammation and Disease. A 2021 Update

Jordi Camps, Helena Castañé, Elisabet Rodríguez-Tomàs, Gerard Baiges-Gaya, Anna Hernández-Aguilera, Meritxell Arenas, Simona Iftimie, Jorge Joven

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: cancer; cardiovascular disease; chemokine (C-C motif) ligand 2; fatty liver; infection; inflammation; metabolism; obesity; paraoxonase-1

Online: 1 June 2021 (10:46:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202003.0292.v1

Lowered Paraoxonase 1 Activities May Explain the Comorbidities Between Temporal Lobe Epilepsy or Mesial Temporal Sclerosis and Comorbid Depression, Anxiety and Psychosis

Ana Paula Michelin, Mchael Maes, Thitiporn Supasitthumrong, Chusak Limotai, Andressa Keiko Matsumoto, Laura de Oliveira Semeão, João Victor de Lima Pedrão, Estefania Gastaldello Moreira, Buranee Kanchanatwan, Décio Sabbatini Barbosa

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: temporal lobe epilepsy; antioxidants; oxidative stress; neuroimmune; major depression; schizophrenia

Online: 19 March 2020 (02:11:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints201609.0024.v1

Identification of One BOCR Mutation and Five NF1 Mutations in Male Patients with Neurofibromatosis Type 1 and Congenital Pseudarthrosis of the Tibia

Zhifan Zhou, Yong Zeng, Xinchun Wu, Fei Li, Xiaoyan Liu, Lu Zhou, Lihua Hou, Silong Sun, Lu Shen, Zhangyuan Lin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: neurofibromatosis type 1; congenital pseudarthrosis of the tibia; whole-exome sequencing; targeted sequencing; BCOR

Online: 7 September 2016 (11:19:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0552.v1

Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

Marcos E. Fernández-Cuadros, Olga S. Pérez-Moro, María J Albaladejo-Florín, Rubén Algarra-López, María M. Tobar-Izquierdo, Amelia Magaña-Sánchez, Patricia Jiménez-Cuevas, Javier Rodríguez-de-Cía

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: ozone; ozone therapy; cytokines; biomarkers; IL-6; inflammation; chronic inflammation; IGF-1; anabolism; knee osteoarthritis; pain; function; c-reactive protein; uric acid

Online: 29 November 2021 (19:08:10 CET)

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Objectives: 1) to demonstrate the anti-inflammatory and anabolic effect of Ozone by determining in serum samples the biochemical levels of IL-6 and IGF-1 in knee osteoarthritis (OA) patients in a real world in Rehabilitation Setting; 2) to evaluate clinical effectiveness by Visual Analog Scale (VAS) and WOMAC scale, and biochemical effect by C-reactive protein (CRP), uric acid and erythrocyte sedimentation rate (ESR). Material and methods: 65 patients with knee OA Kellgren Lawrence (KL) grade 2 or more were analyzed in a retrospective observational study. The study run from January 2018 to September 2021. Inclusion criteria: a) patients 18 years or older; b) with knee OA KL 2º or more; c) biochemical analysis before-and-after treatment; d) pain more than 3 on VAS. Exclusion Criteria: a) previous knee surgery; b) favism; c) pregnancy; d) any other disease that originates lack of collaboration for infiltration. Primary Outcome variables: a) IL-6; b) IGF-1 in diabetes mellitus (DM)/obese and non-DM/non-obese patients; both before-and-after Ozone treatment. Secondary Outcome variables: a) CRP, b) ESR, c) uric acid, d) VAS pain, e) WOMAC pain, function and stiffness. Ozone protocol consisted of 4 sessions (once a week) of an intra-articular infiltration of 20 mL (20µg/mL concentration) of a gas mixture of Oxygen-Ozone 95-5% (produced by Ozone generator Ozonosan-α Plus ®). For biochemical evaluation, SNIBE MAGLUMI ™ IL-6 (CLIA) and SNIBE MAGLUMI ™ IGF-1 (CLIA) kits were used. CRP and uric acid were analyzed by Abbott Alinity c kit; and ESR was evaluated by DIESSE VES MATIC CUBE 30. Results: There is a linear correlation between age and OA severity. IL-6 decreased both in DM and non-DM patients and in all OA KL grades (from 2.7 to 1.59 pg/mL). IGF-1 decreased in total group (OA + DM + obesity) from 112.09 to 107.19 ng/mL. When only knee OA patients were analyzed, Ozone improved IGF-1 levels (from 100.17 to 102.03 ng/mL). Ozone decreased CRP, ESR, uric acid, and improved VAS pain, WOMAC pain, function and stiffness (p<0.05). Conclusions: Ozone is a valid option for the management of knee osteoarthritis in real world Rehabilitation Setting, because of its anti-inflammatory, metabolic and anabolic properties. Ozone downregulates pro-inflammatory IL-6 cytokine. Ozone has a metabolic/hypoglycemic effect on obese/diabetic knee osteoarthritis patients by reducing IGF-1. Ozone has an anabolic effect on non-diabetic/non-obese patients by improving IGF-1. Ozone reduces other biomarkers of inflammation (CRP, ESR and uric acid) and improves, pain, function and quality of life.

Preprint ARTICLE | doi:10.20944/preprints202407.0191.v1

The Burden of Inpatient Hospitalizations with Cardiac and Cerebrovascular Diseases in Patients with Type 1 Diabetes: Insights from the National Inpatient Sample in the US

Chun Shing Kwok, Adnan I. Qureshi, Anne Phillips, Gregory Y.H. Lip, Wasim Hanif, Josip A. Borovac

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: type 1 diabetes mellitus; T1DM; cardiac disease; cerebrovascular disease; disease burden; mortality; inpatient; hospitalization cost

Online: 2 July 2024 (10:55:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0239.v1

Prognostic Value of the Intratumoral Lymphocyte to Monocyte Ratio and M0 Macrophage Enrichment in the Melanoma Immune Microenvironment

Neil Jairath, Mark Farha, Ruple Jairath, Paul Harms, Lam Tsoi, Trilokraj Tejasvi

Subject: Medicine And Pharmacology, Dermatology Keywords: Cutaneous Melanoma; Immunotherapy; Lymphocytes; Monocytes; Macrophages; RNAseq; tumor immune microenvironment

Online: 11 September 2020 (04:02:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0530.v1

Protective Effect of Pyrogallol-phloroglucinol-6,6-bieckol from Ecklonia cava on Monocyte-Associated Vascular Dysfunction

Seyeon Oh, Myeongjoo Son, Hye Sun Lee, Hyun-Soo Kim, You-Jin Jeon, Kyunghee Byun

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: poor blood circulation; Ecklonia cava; phlorotannins; pyrogallol-phloroglucinol-6,6-bieckol; functional ingredients; endothelial cell death; vascular smooth muscle cell proliferation and migration; inflammation

Online: 23 October 2018 (09:39:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1589.v1

Synthesis and Characterization of Recombinant Gelsolin 1-3 for Use in the Detection of Lysophosphatidic Acid, a Possible Ovarian Cancer Biomarker

Navina Lotay, Cynthia Maria Suarez, Brian De La Franier, Rebecca Ann Jockusch, Michael Thompson

Subject: Biology And Life Sciences, Life Sciences Keywords: gelsolin; SDS-PAGE; FTICR-MS; mass spectrometry; protein characterization

Online: 19 July 2024 (10:52:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1102.v1

Relationship Between Perception of Quality of Life and Nutri-tional Status of Mothers Caring For Childrew With Type 1 Diabetes Mellitus

Maria de Nazareth de Lima Carneiro, Arthur Andrade da Fonseca, Rachel Coelho Ripardo, Layla Sandia Cezário Alves, Manuela Maria de lima Carvalhal, Daniela Lopes Gomes

Subject: Public Health And Healthcare, Other Keywords: Quality of life; Health of caregivers; Nutritional status; type 1 diabetes

Online: 17 April 2024 (15:04:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1417.v1

Harnessing the Synergy of SGLT2 Inhibitors and Continuous Ketone Monitoring (CKM) in Managing Heart Failure Among Patients with Type 1 Diabetes

Nicola Tecce, Giorgio De Alteriis, Giulia de Alteriis, Ludovica Verde, Mario Felice Tecce, Annamaria Colao, Giovanna Muscogiuri

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: heart failure; type 1 diabetes; diabetic cardiomyopathy; hospitalization; HFpEF; HFrEF; SGLT2-i; sodium-glucose cotransporter-2 inhibitors; diabetic ketoacidosis; epidemiologic studies; patient education; continuous monitoring of ketone levels; ketonemia

Online: 26 February 2024 (11:54:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1317.v1

Gas Temperature Distribution in the Combustion Chamber of a GTM400 Mod Turbojet Engine Powered by Jet A-1 Fuel and Hydrogen

Łukasz Brodzik

Subject: Physical Sciences, Thermodynamics Keywords: turbojet engine; hydrogen combustion process; combustion chamber

Online: 18 January 2024 (03:29:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2109.v1

Theoretical Study on the Structures and Stabilities of CunZn3O3 (N = 1 – 4) Clusters: Sequential Doping of Zn3O3 Cluster with Cu Atoms

Zhi-Wei Tao, Han-Yi Zou, Hong-Hui Li, Bin Wang, Wen-Jie Chen

Subject: Chemistry And Materials Science, Theoretical Chemistry Keywords: Copper-doped zinc oxide clusters; Density functional theory; Structural evolution; Reactivity

Online: 27 December 2023 (11:52:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.1028.v1

Evaluation of DNA Methylation Profiles of LINE-1, Alu and Ribosomal DNA Repeats in Human Cell Lines Exposed to Radiofrequency Radiation

Francesco Ravaioli, Maria Giulia Bacalini, Cristina Giuliani, Camilla Pellegrini, Chiara D'Silva, Sara De Fanti, Chiara Pirazzini, Gianfranco Giorgi, Brunella Del Re

Subject: Biology And Life Sciences, Life Sciences Keywords: Radiofrequency electromagnetic field; DNA methylation; Neuroblastoma; LINE-1; Alu; ribosomal DNA

Online: 27 April 2023 (04:12:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0128.v1

Epidemiological Description and Molecular Characterization of Intrafamily Transmission of Human T-Lymphotropic Virus Type 1 in an Endemic Region of Brazil

Cássia Pereira, Samir Mansour Moraes Casseb, Akim F.S. Nobre, Maria N. L. Reis, Carlos A. Costa, Louise S. C. Covre, Maisa S. Sousa

Subject: Biology And Life Sciences, Virology Keywords: HTLV; Amazon; Phylogeny

Online: 7 February 2023 (10:47:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints202008.0322.v1

Transporter Associated with Antigen Processing 1 (TAP1) as a Potential Biomarker for Breast, Lung, Liver and Ovarian Cancer using Health Informatics

Anika Tabassum, Md. Nazmus Samdani, Tarak Chandra Dhali, Rahat Alam, Foysal Ahammad, Abdus Samad, Tomasz M. Karpiński

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: TAP1; transcriptional expression; methylation analysis; survival analysis; co-expression; pathway analysis; health informatics

Online: 14 August 2020 (11:17:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201907.0048.v1

Vitamin D and ω-3 Supplementations in Mediterranean Diet During the 1st Year of Overt Type 1 Diabetes: A Cohort Study

Francesco Cadario, Erica Pozzi, Stefano Rizzollo, Marta Stracuzzi, Sheila Beux, Alberto Giorgis, Deborah Carrera, Fabiola Fullin, Sergio Riso, Angela Maria Rizzo, Gigliola Montorfano, Marco Bagnati, Umberto Dianzani, Philippe Caimmi, Gianni Bona, Camillo Ricordi

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: cholecalciferol; omega3; EPA; DHA; arachidonic acid; AA/EPA ratio; type 1 diabetes; remission period; honeymoon period

Online: 3 July 2019 (08:10:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0211.v1

Microscopic Views of Atomic and Molecular Oxygen Bonding with epi Ge(001)-2×1 Studied by High-Resolution Synchrotron Radiation Photoemission

Yi-Ting Cheng, Hsien-Wen Wan, Chiu-Ping Cheng, Jueinai Kwo, Minghwei Hong, Tun-Wen Pi

Subject: Physical Sciences, Applied Physics Keywords: Ge(001)-2×1; oxidation; synchrotron radiation photoemission

Online: 22 February 2019 (04:07:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints201811.0334.v1

Identification of Novel Inhibitors of Pteridine Reductase 1 in Trypanosoma brucei via Computational Structure-Based Approaches and In Vitro Inhibition Assays

Magambo Phillip Kimuda, Dustin Laming, Heinrich C. Hoppe, Özlem Tastan Bishop

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: human African trypanosomiasis; pteridine reductase 1; PTR1; DHFR; antifolates; anti-trypanosomal agents; molecular dynamics; dynamic residue network analysis; binding free energy; isobologram assay

Online: 14 November 2018 (10:39:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1169.v1

Puzzling Regulation of Interferon Signaling System by p53 Tumor Suppressor Protein

Agnieszka Będzińska, Barbara Łasut-Szyszka, Małgorzata Krześniak, Agnieszka Gdowicz-Kłosok, Marek Rusin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: p53; interferon; STAT1; NK-92; caspase-1; innate immunity

Online: 17 April 2024 (14:47:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0953.v1

High-Resolution Land Use Land Cover Dataset for Meteorological Modelling – Part 1: ECOCLIMAP-SG+ an Agreement-Based Dataset

Geoffrey Bessardon, Thomas Rieutord, Emily Gleeson, Sandro Oswald, Bolli Palmason

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: land cover land use; meteorology; uncertainty quantification

Online: 12 September 2024 (13:14:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2237.v1

Potent Anti-HIV-1 Activity of Sacran, a Megamolecular Polysaccharide from Cyanobacterium Aphanothece sacrum, as a Microbicide Candidate

Kouki Matsuda, Ryusho Kariya, Kenji Maeda, Seiji Okada

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sacran; HIV-1; microbicide

Online: 30 July 2024 (08:57:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.1533.v1

Exploring the Role of PD-1 in the Autoimmune Response: Insights into Its Implication in Systemic Lupus Erythematosus

Nefertari Sagrero-Fabela, Ramón Chávez-Mireles, Diana Celeste Salazar-Camarena, Claudia Azucena Palafox-Sánchez

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: PD-1; PD-L1/PD-L2; Systemic lupus erythematosus; T follicular helper (Tfh) cells; T peripheral helper (Tph) cells.

Online: 21 June 2024 (16:52:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0762.v1

Explore the Molecular Mechanism of Caveolae-1 Regulates Changes in Blood-Brain Barrier Permeability Leading to Eosinophilic Meningoencephalitis

An-Chih Chen, Shih-Chan Lai, Cheng-You Lu, Ke-Min Chen

Subject: Medicine And Pharmacology, Tropical Medicine Keywords: Angiostrongylus cantonensis; Caveolae-1; blood-brain barrier permeability; cerebral edema

Online: 11 April 2024 (13:59:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0491.v1

Nature-Inspired 1-phenylpyrrolo[2,1-A]isoquinoline Scaffold for Novel Antiproliferative Agents Circumventing P-Glycoprotein-Dependent Multidrug Resistance

Alisa A. Nevskaya, Rosa Purgatorio, Arina Obydennik, Lada Anikina, Elena Yu. Nevskaya, Tatiana N. Borisova, Alexey V. Varlamov, Mauro Niso, Nicola Antonio Colabufo, Antonio Carrieri, Marco Catto, Modesto De Candia, Leonid G. Voskressensky, Cosimo D. Altomare

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: pyrrolo[2,1-a]isoquinolines; Mannich bases; cytotoxicity; P-glycoprotein; inhibition; multidrug resistance reversal.

Online: 8 March 2024 (04:15:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1627.v1

Natural Gas Sweetening via Membrane-Assisted Gas Absorption. Part 1: A Hollow-Fiber Unit with MDEA-Based Absorbent

Anton Petukhov, Artem Atlaskin, Kirill Smorodin, Mariia Atlaskina, Dmitriy Mikhailovich Zarubin, Nikita S. Tsivkovsky, Sergey Kryuchkov, Anna Stepakova, Andrey Vorotyntsev, Olga Kazarina, Sergey Suvorov, Ekaterina Stepanova, Ilya Vorotyntsev

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: membrane-assisted gas absorption; natural gas processing; sweetening; gas separation; hollow fibers

Online: 21 December 2023 (08:37:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0389.v1

High Mobility Group Box-1 as a Candidate Biomarker of Portal Vein Thrombosis in Patients with Hepatocellular Carcinoma

Mattia Perazzi, Roberto Piffero, Rosalba Minisini, Silvia Gaia, Emanuela Rolle, Gian Paolo Caviglia, Michela Emma Burlone, Giulia Francesca Manfredi, Stelvio Tonello, Mattia Bellan, Patrizia Carucci, Mario Pirisi

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: High Mobility Group Box-1 (HMGB-1); portal vein thrombosis (PVT); hepatocellular carcinoma (HCC)

Online: 7 November 2023 (10:36:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0594.v1

Monitoring Braided River-Bed Dynamics at Sub-Event Time Scale Using Time Series of Sentinel-1 SAR Imagery

Daniele Rossi, Guido Zolezzi, Walter Bertoldi, Alfonso Vitti

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: SAR; Gravel-bed rivers; Morphodynamics; Flood dynamics; River bank erosion

Online: 8 June 2023 (05:00:07 CEST)

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Remote sensing plays a central role in the assessment of environmental phenomena and has increasingly become a powerful tool for monitoring shorelines, rivers morphology, flood waves delineation and floods assessment. Optical based monitoring and characterization of river evolution at long time scales is a key tool in fluvial geomorphology. However, the evolution occurring during extreme events is crucial for the understanding of the river dynamics under severe flow conditions and requires the processing of data from active sensors to overcome cloud obstructions. This work proposes a cloud-based unsupervised algorithm for the intra-event monitoring of river dynamics during extreme flow conditions based on time series of Sentinel-1 SAR data. The method allows the extraction of multi-temporal series of spatially explicit geometric parameters at high time and spatial resolutions, linking them to the hydrometric levels acquired by reference gauge stations. Intra-event reconstruction of inundation dynamics has led to the estimation of the relationship between hydrometric level and wet area extension and the assessment of bank erosion phenomena. Time series of SAR acquisitions, provided by Copernicus Sentinel-1 satellites, were analyzed to quantify changes in the wet area of a reach of the Tagliamento river under different flow conditions. The algorithm, developed within the Python-API of GEE, first empowers the Sentinel-1 images with the hydrometric level, then involves radiometric slope correction and speckle noise filtering. The Otsu method is then used for image segmentation leading to a water and dry land binary classification. Results support many types of analysis about river dynamics, including morphological changes, floods monitoring and relief efforts and bio-physical habitat dynamics. The results encourage future advancements and applications of the algorithm, specifically exploring SAR data from ICEYE and Capella Space constellations, which offer significantly higher spatial and temporal resolutions compared to Sentinel-1 data.

Preprint ARTICLE | doi:10.20944/preprints202303.0399.v1

A Novel Anti-CD44 Variant 9 Monoclonal Antibody C₄₄Mab-1 was Developed for Immunohistochemical Analyses Against Colorectal Cancers

Mayuki Tawara, Hiroyuki Suzuki, Tomohiro Tanaka, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CD44; CD44v9; monoclonal antibody; colorectal cancer

Online: 22 March 2023 (14:21:41 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202303.0309.v1

Identification of the Binding Epitope of an Anti-Mouse CCR6 Monoclonal Antibody (C6Mab-13) Using 1×Alanine Scanning

Tomohiro Tanaka, Mayuki Tawara, Hiroyuki Suzuki, Mika K. Kaneko, Yukinari Kato

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mouse CCR6; monoclonal antibody; epitope; ELISA; SPR

Online: 16 March 2023 (14:13:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0063.v1

Everything you Always Wanted to Know about the Biothermodynamic Background of Herpes Simplex Virus Type 1 – Host Interaction

Marko Popovic

Subject: Biology And Life Sciences, Virology Keywords: Herpes simplex virus type 1 (HSV-1); antigen-receptor binding; equilibrium constant; Gibbs energy of binding; biothermodynamics

Online: 5 December 2022 (07:07:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202204.0053.v1

Computational Investigations on the Natural Small-Molecule as an Inhibitor of Programmed Death Ligand-1 for Cancer Immunotherapy

Geethu S. Kumar, Mahmoud Moustafa, Amaresh Kumar Sahoo, Petr Malý, Shiv Bharadwaj

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Programmed death ligand-1; Natural products; Immunotherapy; Neoenactin B1; Molecular dynamics simulation

Online: 7 April 2022 (04:08:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0217.v1

Accuracy Assessment of the GlucoMen® Day CGM System in Individuals with Type 1 Diabetes: A Pilot Study

Daniel A Hochfellner, Amra Simic, Marlene T Taucher, Lea S Sailer, Julia Kopanz, Tina Pöttler, Julia K Mader

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Diabetes Technology; CGM; Accuracy; Type 1 Diabetes; Sustainability

Online: 12 November 2021 (11:58:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints202006.0166.v2

The Effect of a Color Tattoo on the Local Skin Redox Regulatory Network: An N-of-1 Study

Jan Homolak

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Tattoo; Tattoo ink; Oxidative stress; Phthalocyanine; Skin

Online: 2 December 2020 (11:05:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202005.0336.v1

Extraction of Sea Ice Cover by Sentinel-1 SAR Based on SVM with Unsupervised Generation of Training Data

Xiaoming Li, Yan Sun, Qiang Zhang

Subject: Environmental And Earth Sciences, Oceanography Keywords: synthetic aperture radar; sea ice; machine learning

Online: 21 May 2020 (05:59:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202001.0130.v1

Inter-Molecular Electrostatic Interactions Stabilizing the Structure of the PD-1/PD-L1 Axis: A Structural Evolutionary Perspective

Wei Li

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: PD-1; PD-L1; Cancer-linked genetic mutation; Electrostatic repulsion; Structural evolution

Online: 12 January 2020 (16:58:35 CET)

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Working Paper ARTICLE

Ultrasonic Transceiver with a Regular/Periodic 1-3 Piezocomposite Based on the SAW Resonance Mode on Damping Backing

Alex Mezheritsky

Subject: Engineering, Electrical And Electronic Engineering Keywords: piezoelectric materials; piezoelement; 1-3 piezocomposite; resonance and antiresonance frequencies; quality Q-factor; surface acoustic wave (SAW); interdigital transducer (IDT); mass load; SAW resonator

Online: 31 December 2019 (16:01:57 CET)

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A novel effective vibrational mode was discovered in the conventional transducer with an array of orthogonal (square) regular piezoelectric rods in 1-3 piezocomposite, containing the damping backing and front matching layers. The operational resonance in the structure was determined as the Surface Acoustic Wave (SAW) on the backing boundary excited by the adjacent piezo-rods, with its frequency typically near 3 times lower the fundamental half-lambda conventional piezocomposite resonance. Pulse-echo sensitivity and transmitting sound-pressure-level (SPL) in air showed that the signal strength is roughly comparable to the industrial similar air transducers at the frequency range 100…700 kHz, where at these frequencies the lateral and longitudinal piezoelement dimensions in the conventional transducer design are typically close to each other causing interference with unwanted coupling modes. As was determined theoretically and proved in experiments, the backing SAW resonance effect in the transducer performance is inherent just to the regular periodic 1-3 piezocomposite structure, and does occur neither with randomly located/oriented piezo-rods, nor in the homogeneous piezo-plate at least with the same lateral cross-section as the connected to it backing. The purpose of the article is to investigate a newly discovered operational vibrational mode of a SAW type in 1-3 regular piezocomposite, other than piezoelectric resonance. The investigated phenomena can improve the transceiver sensitivity, bandwidth, providing lower drive voltage, and smaller and lighter weight ultrasonic transducers. Based on the piezocomposites with thickness’ 1…1.5 mm (rod resonance near 2…3 MHz), pillar width 0.2…0.8 mm, kerf width 0.1…0.4 mm, the transceivers with an operating frequency from 140 kHz to 650 kHz were designed and fabricated with a conventional backing of a mixture of high-density tungsten powder and epoxy, and a matching layer of a mixture of low-density glass bubbles and epoxy. Experimental evaluation of their acoustical performance showed expected characteristics suitable for practical applications.

Working Paper ARTICLE

Dichotomy in Fatal Outcomes in a Large Cohort of People Living with HTLV-1 in São Paulo, Brazil

Rosa Maria N. Marcusso, Johan Van Weyenbergh, João Victor Luisi de Moura, Flávia Esper Dahy, Tatiane Assone, Michel Y. Jung Haziot, José Ernesto Vidal, Luiz Augusto M. Fonseca, Jerusa Smid, Aline de Moura Brasil Matos, Jorge Casseb, Augusto Penalva Oliveira

Subject: Biology And Life Sciences, Virology Keywords: htlv-1; mortality, ham/tsp; Brazil

Online: 13 November 2019 (10:40:50 CET)

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BACKGROUND: During the natural history, the incidence HTLV-1 related diseases were 0.5% until 10% after decades of infection. Despite relative low lethality, previous study observed that HTLV-1 infection is associated with significantly increased mortality. For example, the incidence of ATLL and HAM/TSP, co-infections with HIV and HCV, parasitic co-infection with Strongyloides stercoralis may increase morbidity and mortality. OBJECTIVE: Determine the mortality rate and its major variables as possible risk factors, analyzing the HTLV Clinic at Emilio Ribas Institute, a continuous open cohort of HTLV patients since 1997. METHODS: This cohort open cohort possesses new patients added at a rate of approximately 50 patients/year. There were 1100 HTLV-infected subjects identified by September 30th 2018. All clinical data including clinical and laboratory data, which have been updated on a regular basis over the last 22 years, were entered into a specific REDCap database. The Ethical Board of the IIER approved the protocol. RESULTS: Along 22 years of clinical care in the HTLV-out clinic, 727 HTLV-1-infected subjects and 248 HAM/TSP cases. Four-hundred sixty-eight patients of whom remaining under active follow up. The mean follow-up time of the cohort was 12 years. Twenty-seven patients died (median age of 51,5 years old). From this total, 13 was asymptomatic carriers and 14 HAM/TSP subjects, 12 of asymptomatic and seven HAM/TSP possess co-infected with HIV or/and HCV. Other seven presented HAM/TSP without co-infection, and all was female, and sepsis was the majority cases the majority of cases of sepsis were related to infection of the urinary tract (p=0.058) and to ulcers of decubitus (p=0.021), and it was associated with risk factors for mortality. CONCLUSIONS: Overall, the mortality rate for HAM-TSP patients was six percent. The mean life expectancy in Brazil is about 78 years in 2018, twenty-two years higher than HAM/TSP patients without any other coinfection. This study highlights the possibility of higher death risk among HTLV-1-infected patients and HAM/TSP subjects in Brazil, and identified some risk factors for this outcome. Also, these patients may possess higher risk for morbidity, usually urinary tract infections and decubitus scars, which could increase risk for death.

Preprint ARTICLE | doi:10.20944/preprints201901.0020.v1

Inhibition of Osteopontin Synthesis in THP-1 Cells Stimulated with Phorbol 12-Myristate 13-Acetate by Brefelamide Derivatives

Gaowa Bai, Takashi Matsuba, Haruhisa Kikuchi, Hirotoshi Motoda, Ryo Ozuru, Haorile Chagan-Yasutan, Osamu Yamada, Yoshiteru Oshima, Toshio Hattori

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: osteopontin; THP-1; brefelamide; inhibitor; tuberculosis

Online: 3 January 2019 (13:07:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0126.v1

Analysis of Chemically Labile Glycation Adducts in Seed Proteins: Case Study of Methylglyoxal-Derived Hydroimidazolone 1 (MG-H1)

Kristina Antonova, Maria Vikhnina, Alena Soboleva, Tahir Mehmood, Marie-Louise Heymich, Tatiana Leonova, Mikhail Bankin, Elena Lukasheva, Sabrina Gensberger-Reigl, Sergei Medvedev, Galina Smolikova, Monika Pischetsrieder, Andrej Frolov

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: advanced glycation end products (AGEs); enzymatic hydrolysis; glycation; methylglyoxal-derived hydroimidazolone 1 (MG-H1); seeds; seed ageing; seed quality; sodium dodecyl sulfate (SDS)

Online: 11 December 2018 (10:40:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0263.v1

Precision Permittivity Measurement for Low-Loss Thin Planar Materials Using Large Coaxial Probe from 1 to 400 MHz

Kok Yeow You, Man Seng Sim

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: large coaxial probe; thin planar materials; low-loss materials; relative permittivity; reflection coefficient; calibration

Online: 12 October 2018 (08:19:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201805.0380.v1

Computational Insight into the Effect of Natural Compounds on the Destabilization of Preformed Amyloid-β(1-40) Fibrils

Francesco Tavanti, Alfonso Pedone, Maria Cristina Menziani

Subject: Chemistry And Materials Science, Theoretical Chemistry Keywords: molecular dynamics simulation; biophenols; natural compounds; amyloid fibrils; Alzheimer’s disease; ligand-protofiber interactions

Online: 27 May 2018 (13:09:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0445.v1

HIV Protein Nef Induces Cardiomyopathy through Induction of Bcl2 and p21

Olena Kondrachuck, Pierce Ciccone, Nicole Ford, Kim Hong, Yuka Kimura, Jorgo Zi, Sumaya Yusuf, Aya Alkousa, Nishit Tailor, Rithvik Rajakumar, Jay Rappaport, Manish Gupta

Subject: Biology And Life Sciences, Aging Keywords: HIV; Nef; autophagy; cardiomyopathy; Bcl2; Beclin-1; fibrosis; P21

Online: 5 September 2024 (11:00:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1267.v1

Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases

Jonathan Sundstrom, Emma Vanderleeden, Nathaniel J Barton, Sambra D Redick, Pepper Dawes, Liam F Murray, Meagan N Olson, Khanh Tran, Samantha M Chigas, Adrian R Orszulak, George M Church, Benjamin Readhead, HyungSuk Oh, David M Harlan, David M Knipe, Jennifer P Wang, Yingleong Chan, Elaine T Lim

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: stem cell islets; cerebral organoids; Type 1 diabetes; Alzheimer’ s disease; innate immune; neurodegenerative diseases; autoimmune diseases; acyclovir; herpes simplex virus 1

Online: 16 July 2024 (08:27:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1376.v1

Circulation of Dengue Virus Serotype 1 genotype V and Dengue Virus Serotype 2 genotype III in Tocantins State, Northern Brazil, 2021-2022

Ueric José Borges de Souza, Ygor da Silva Miranda Macedo, Raíssa Nunes dos Santos, Franciano Dias Pereira Cardoso, Jucimária Dantas Galvão, Evgeni Evgeniev Gabev, Ana Cláudia Franco, Paulo Michel Roehe, Fernando Rosado Spilki, Fabrício Souza Campos

Subject: Biology And Life Sciences, Virology Keywords: DENV; Genomic surveillance; Epidemiology; molecular clock; SNPs; arboviruses

Online: 20 September 2023 (10:42:22 CEST)

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The dengue virus (DENV) is an arbovirus belonging to the Flaviviridae family; the species comprises four antigenically distinct serotypes (DENV–1, DENV–2, DENV–3, and DENV–4), which are further subdivided into genotypes. The virus is transmitted to humans through mosquito bites, primarily from the Aedes spp. genus. Dengue is endemic in various parts of the world, including tropical and subtropical regions of Asia, Latin America, Africa, and Oceania. In Brazil, the state of Tocantins, located in north-central Brazil, has experienced a significant number of arboviral disease cases, particularly dengue. This study aimed to monitor DENV circulation within the state by conducting full genome sequencing of viral genomes recovered from 61 patients between June 2021 and July 2022. During this period, both DENV-1 and DENV-2 serotypes were identified. Our findings confirm the circulation of DENV serotypes 1 and 2 in Tocantins, affecting males and females equally, with younger age groups (4 to 43 years old) being the most susceptible. Phylogenetic analysis revealed that the circulating viruses belong to DENV–1 genotype V American and DENV–2 genotype III Southeast Asian/American. The Bayesian analysis of DENV-1 Genotype V sequenced here is closely related to genomes previously sequenced in the state of São Paulo. Regarding DENV-2 genotype III genomes, these are clustered in a distinct, well-supported subclade, along with previously reported isolates from the states of Goiás and São Paulo. In both cases, our results suggest that multiple introductions of these genotypes occurred in the Tocantins state. This observation highlights the significant impact of major population centers in Brazil on virus dispersion, including other Latin-American countries and the USA. In the SNP analysis, DENV-1 displayed 122 distinct missense mutations, while DENV-2 had 44, with significant mutations predominantly occurring in the envelope and NS5 proteins. The analyses performed here reveal the concomitant circulation of distinct DENV-1 and -2 genotypes in some Brazilian states, underscoring the dynamic evolution of the DENV and the ongoing significance of surveillance efforts in supporting public health policies.

Preprint REVIEW | doi:10.20944/preprints202308.1332.v1

Human T-cell Leukemia Virus Type 1 Oncogenesis between Active Expression and Latency: A Possible Source for the Development of Therapeutic Targets

Francesca Marino-Merlo, Sandro Grelli, Antonio Mastino, Michele Lai, Paola Ferrari, Andrea Nicolini, Mauro Pistello, Beatrice Macchi

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HTLV-1; adult T-cell leukemia; viral oncogenesis; Tax; HBZ; apoptosis

Online: 18 August 2023 (09:41:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0561.v1

Immunotherapy with PD-1 Inhibitor Nivolumab in Recurrent/Metastatic Platinum Refractory Head and Neck Cancers: The Early Experience of Two Academic Center

Camil Ciprian Mireștean, Mihai Cosmin Stan, Michael Schenker, Constantin Volovăț, Simona Ruxandra Volovăț, Dragoș Petru Teodor Iancu, Roxana Irina Iancu, Badulescu Florinel

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Nivolumab; immunotherapy; head and neck cancers; HNSCC; chemotherapy; neutrophil to lymphocyt ratio; NLR; second primary malignancy; Cetuximab

Online: 9 May 2023 (04:21:38 CEST)

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The prognosis of patients with recurrent or metastatic of the head and neck squamous-cell carcinoma (HNSCC) refractory to platinum-based chemotherapy is severe and, consequently, the identification of therapeutic options for this category of patients is a priority. Nivolumab, an anti- programmed cell death protein 1 (anti-PD-1) monoclonal antibody, has been approved for the treatment of recurrent or metastatic HNSCC after platinum-based therapy progressing. We present the early experience of two academic center including diagnostic, clinical, biological, therapeutic and outcomes characteristics of head and neck cancer (HNC) patients treated with Nivolumab. The purpose of the study is to identify certain peculiarities and to report them compared to the data from the literature in order to generate some hypotheses that coud constitute criteria for the selection of cases with maximum benefit to immunotherapy. Analyzing the data obtained from 18 patients treated in Emergency County Hospital Craiova, "Saint Nectarie" Oncological Hospital Craiova and Euroclinic Oncological Center Iasi January 2020 and March 2023 it could be hypothesized that lower nadir values of neutrophil to lymphocyte ratio (<3.5) and the adition of intensive regimens of chemotherapy and radiotherapy could justify a favorable response in terms of progression free survival (PFS) during immunotherapy with Nivolumab. To our knowledge, the study included the first two cases of second primary malignancy (SPM) in the head neck region treated with Nivolumab. The reported data support a possible benefit of the sequential administration of radiotherapy, immunotherapy for SPM cases. Higher positive dynamic of neutrophil to lymphocyt ratio (NLR) could be associated with worst outcome during immunotherapy. All study observations including the role of infections and antibiotic treatment as well as the possible biomarker value of NLR variation during immunotherapy should be investigated in multicenter clinical trials.

Preprint ARTICLE | doi:10.20944/preprints202301.0562.v1

Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission. Study 1

Elena Blázquez, Joan Pujols, Fernando Rodriguez, Joaquim Segalés, Rosa Rosell, Joy Campbell, Javier Polo

Subject: Biology And Life Sciences, Virology Keywords: African swine fever; ASFV; spray-dried porcine plasma; challenge; nutritional intervention

Online: 31 January 2023 (02:21:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0170.v1

Low-intensity Pulsed Ultrasound-mediated Blood-brain Barrier Opening Increases Anti-programmed Death-ligand 1 Delivery and Efficacy in Gl261 Mouse Model

Mohammed H Ahmed, Emie Quissac, Nolwenn Lemaire, Isaias Hernández-Verdín, Noël Zahr, Charlotte Schmitt, Guillaume Bouchoux, Michael Canney, M.D. Santin, Alexandra Petiet, Laura Mouton, Coralie Guerin, Lea Guyonnet, Marc Sanson, Maïté Verreault, Alexandre Carpentier, Ahmed Idbaih

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: SonoCloud; GL261 mouse model; ultrasound-mediated drug delivery; glioblastoma; immune checkpoint inhibitors

Online: 9 December 2022 (02:59:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0120.v1

Mathematical Modeling of the Growth Suppression of HER2+ Breast Cancer Colonies Under the Effect of Trastuzumab and PD-1/PD-L1 Inhibitor

Regina Padmanabhan, Hadeel Kheraldine, Ishita Gupta, Nader Meskin, Anas Hamad, Semir Vranic, Ala-Eddin Al Moustafa

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; PD-1/PD-L1; Mathematical model; HER2/PD-1 Interaction; Breast cancer

Online: 9 May 2022 (14:06:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0096.v1

Interplay among calcium diet content, PTH(1-34) treatment and balance of bone homeostases in rat model: the trabecular bone as keystone

Marzia Ferretti, Francesco Cavani, Laura Roli, Marta Checchi, Maria Sara Magarò, Jessika Bertacchini, Carla Palumbo

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: calcium diet content; mineral homeostasis; skeletal homeostasis; trabecular bone; PTH(1-34); rat

Online: 10 January 2019 (11:38:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0456.v1

The salt-stress response of the transgenic plum line J8-1 and its interaction with the salicylic acid biosynthetic pathway from mandelonitrile

Agustina Bernal-Vicente, Daniel Cantabella, Cesar Petri, Jose Antonio Hernández, Pedro Díaz-Vivancos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: chlorophyll fluorescence; J8-1 plum line; mandelonitrile; Prunus domestica; redox signalling; salicylic acid; salt-stress; soluble nutrients

Online: 19 October 2018 (14:57:02 CEST)

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Salinity is considered as one of the most important abiotic challenges that affect crop productivity. Plant hormones, including salicylic acid (SA), are key factors in the defence signalling output triggered during plant responses against environmental stresses. We have previously reported in peach a new SA biosynthetic pathway from mandelonitrile (MD), the molecule at the hub of the cyanogenic glucoside turnover in Prunus sp. In this work, we have studied whether this new SA biosynthetic pathway is also present in plum and the possible role this pathway plays in plant plasticity under salinity, focusing on the transgenic plum line J8-1, which displays stress tolerance via an enhanced antioxidant capacity. The SA biosynthesis from MD in non-transgenic and J8-1 micropropagated plum shoots was studied by metabolomics. Then the response of J8-1 to salt stress in presence of MD or Phe (MD precursor) was assayed by measuring: chlorophyll content and fluorescence parameters, stress related hormones, levels of non-enzymatic antioxidants, the expression of two genes coding redox-related proteins, and the content of soluble nutrients. The results from in vitro assays suggest that the SA synthesis from the MD pathway demonstrated in peach is not clearly present in plum, at least under in vitro conditions. Nevertheless, in J8-1 NaCl-stressed seedlings, an increase in SA was recorded as a result of the MD treatment, suggesting that MD could be involved in the SA biosynthesis under NaCl stress conditions in plum plants. We have also shown that the plum line J8-1 was tolerant to NaCl under greenhouse conditions, and this response was somewhat different in MD-treated plants. In that regards, the MD treatment produced an increase in SA, jasmonic acid (JA) and reduced ascorbate (ASC) contents as well as in the coefficient of non-photochemical quenching (qN) and the gene expression of Non-Expressor of Pathogenesis-Related 1 (NPR1) and thioredoxin H (TrxH) under salinity conditions, suggesting a crosstalk between different signalling pathways (NPR1/Trx and SA/JA) leading to salinity tolerance in the transgenic plum line J8-1.

Preprint ARTICLE | doi:10.20944/preprints201701.0050.v1

Interleukin-1 Receptor Antagonist (IL1RA) and Interleukin-4 (IL4) Genes Variable Number Tandem Repeats (VNTRs) Are Associated with Adiposity in Malaysian Subjects

Yung-Yean Kok, Hing-Huat Ong, Yee-How Say

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Interleukin-1 receptor antagonist (IL-1Ra); Interleukin-4 (IL-4); Variable Number Tandem Repeat (VNTR); obesity; adiposity; Malaysia.

Online: 10 January 2017 (10:27:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1268.v1

Is Water the Engine of Protein Folding?

Marco Campanile, Giuseppe Graziano

Subject: Biology And Life Sciences, Biophysics Keywords: water; globular proteins; folding; hydrophobic effect

Online: 17 September 2024 (08:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1508.v1

LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

Imene Arab, Su-Geun Lim, Kyoungho Suk, Won-Ha Lee

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: LINC01270; NF-κB signaling; miR-326; LDOC1; Inflammation.

Online: 24 June 2024 (05:50:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1536.v1

Knockdown of DJ-1 Resulted in a Coordinated Activation of the Innate Immune Antiviral Response in HEK293 Cell Line

Keren Zohar, Michal Linial

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cytokine release; Interferon; RNA-seq; oxidative stress; dsRNA; siRNA; innate immunity

Online: 24 June 2024 (04:17:57 CEST)

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PARK7, also known as DJ-1, plays a critical role in protecting cells by functioning as a sensitive oxidation sensor and modulator of antioxidants. DJ-1 acts to maintain mitochondrial function and regulate transcription in response to different stressors. In this study, we showed that cell lines vary based on their antioxidation potential at basal conditions. The transcriptome of HEK293 cells was tested following knockdown (KD) of DJ-1 using siRNAs which reduced the DJ-1 transcripts to only 12% of the original level. We compared the expression levels of 14k protein-coding transcripts, and 4.2k non-coding RNAs relative to control cells treated with non-specific siRNAs. Among the coding genes, ~200 upregulated differentially expressed genes (DEGs) signified a coordinated antiviral innate immune response. Most genes were associated with regulation of type 1 interferons (IFN) and induction of inflammatory cytokines. About a quarter of these genes were also induced in cells treated by non-specific siRNAs that were used as a negative control. Beyond the antiviral response, 114 genes were specific to KD of DJ-1 with enrichment in RNA metabolism and mitochondrial functions. A smaller set of downregulated genes (58 genes) were associated with dysregulation in membrane structure, cell viability, and mitophagy. We propose that KD of DJ-1 diminish its protective potency against oxidative stress, rendering the cells labile and responsive to dsRNA signal by activation of a large number of genes, many of which drive apoptosis, cell death, and inflammatory signatures. The KD of DJ-1 highlights its crucial role in regulating genes associated with antiviral responses, RNA metabolism, and mitochondrial functions, apparently through alteration in STAT activity and downstream signaling. Given that DJ-1 is highly expressed in metastatic cancers, targeting DJ-1 could be a promising therapeutic strategy where manipulation of DJ-1 levels may reduce cancer cell viability and enhance the efficacy of cancer treatments.

Preprint ARTICLE | doi:10.20944/preprints202310.2072.v1

The Enhanced Wagner-Hagras OLS-BP Hybrid Algorithm for Training IT3 NSFLS-1 for Temperature Prediction in HSM Processes

Gerardo Maximiliano Mendez, Ismael Lopez Juarez, Maria Aracelia Alcorta Garcia, Dulce Citlalli Martinez Peon, Pascual Noradino Montes Dorantes

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Interval type-3 fuzzy logic systems; hybrid learning; backpropagation method; orthogonal least square method; general type-2 fuzzy logic systems.

Online: 31 October 2023 (10:33:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0046.v1

Genetic Characterization of HIV-1 tat Gene from Virologically Controlled HIV-infected Older Patients on Long-term Antiretroviral Therapy

Nathan Kummet, Neha Mishra, Adela Diaz, Nicholas Cusick, Stephen A. Klotz, Nafees Ahmad

Subject: Biology And Life Sciences, Virology Keywords: HIV+ older patients; ART; tat sequences; genetic diversity; CTL epitopes

Online: 2 October 2023 (11:55:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1874.v1

Repeatability of Ad Libitum Fluid Intake During Repeated 1 h Walking/Jogging Exercise Sessions Conducted Under Hot Ambient Conditions

Eric D.B. Goulet, Pascale Claveau, Ivan L. Simoneau, Thomas A. Deshayes, Antoine Jolicoeur-Desroches, Fedi Aloui, Martin D. Hoffman

Subject: Biology And Life Sciences, Other Keywords: dehydration; drinking pattern; exercise; heat stress; physiological and perceptual functions

Online: 27 September 2023 (10:36:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1428.v1

The Bacteriocin-Producing Strain Lacticaseibacillus Paracasei LPa 12/1 from Raw Goat Milk, a Potential Additive in Dairy Products

Andrea Lauková, Emília Dvorožňáková, Miroslava Vargová, Jana Ščerbová, Valentína Focková, Iveta Plachá, Monika Pogány Simonová

Subject: Biology And Life Sciences, Food Science And Technology Keywords: lacticaseibacilli; benefit; yoghurt; supplement; functional food

Online: 22 August 2023 (07:44:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1761.v1

Psycho-Behavioral Characteristics Perceived as Facilitators by Brazilian Adults with Type 1 Diabetes Mellitus in a Public Health Service

Priscila Pecoli, Anderson Rosa, Mônica Gabbay, Sérgio Dib

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: type 1 diabetes mellitus; qualitative research; patient experience; self-management; diabetes care

Online: 26 July 2023 (10:54:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1640.v1

Comparative Study of Magnetic Properties of (Mn$_{1-x}$A$_x^{IV}$)Bi2Te4 A$_x^{IV}$ = Ge, Pb, Sn

Dmitry A. Estyunin, Anna A. Rybkina, Konstantin A. Kokh, Oleg E. Tereshchenko, Marina V. Likholetova, Alexander M. Shikin

Subject: Physical Sciences, Condensed Matter Physics Keywords: Topological insulators; Modification of magnetic properties; Antiferromagnetics; Doping; SQUID magnetometry; ARPES

Online: 25 July 2023 (09:29:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202303.0196.v1

Identification of Groundwater Sources in NO.1 Reverside Sliding Mass of Huangtupo Landslide Using Environmental Stable Isotopes as Tracers

Shen Cao, Wei Xiang, Jinge Wang, Deshan Cui, Qingbing Liu

Subject: Environmental And Earth Sciences, Geography Keywords: Landslide; Slope groundwater source; Tracer; Environmental stable isotopes; Huangtupo; Three Gorges Reservoir

Online: 10 March 2023 (13:18:35 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202301.0067.v1

Quick Report of the ML=3.3 on 1 January 2023 Guidonia (Rome, Italy) Earthquake: Evidence of a Seismic Acceleration

Dedalo Marchetti, Kaiguang Zhu, Laura Marchetti, Yiqun Zhang, Wenqi Chen, Yuqi Cheng, Mengxuan Fan, Siyu Wang, Ting Wang, Jiami Wen, Donghua Zhang, Hanshuo Zhang

Subject: Environmental And Earth Sciences, Geophysics And Geology Keywords: seismic acceleration; Guidonia; earthquake; Benioff cumulative stress; Swarm; pre-earthquake processes

Online: 4 January 2023 (06:08:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0353.v1

In Silico Comparative Analysis of Predicted B-Cell Epitopes against Dengue Virus (Serotypes 1-4) Isolated from the Philippines

Lyn Marielle I. Abesamis, Evan Gilles A. Aliping, Fritz Khrystian Gabriel H. Armada, Mirriam F. Danao, Pamela Denise B. del Valle, Zypher Jude G. Regencia, Emmanuel S. Baja, Antonio D. Ligsay

Subject: Biology And Life Sciences, Virology Keywords: Dengue; Epitope; Peptide; B cell; DENV-1; DENV-2; DENV-3; DENV-4; Vaccine

Online: 25 May 2022 (11:26:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0053.v1

Antibiotic Consumption Patterns in European Countries Might Be Associated with the Prevalence of Diabetes Type-1-2 (T1D, T2D)

Gábor Ternák, Márton Németh, Martin Rozanovic, Lajos Bogár

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: diabetes type-1; T1D; diabetes type-2; T2D; antibiotics; antibiotic classes; microbiome; dysbiosis; prevalence; concordance

Online: 3 December 2021 (12:45:23 CET)

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Abstract: Several publications have raised the issue that the development of diabetes is preceded by alteration of the microbiome (dysbiosis) and hence, the role of environmental factors, triggering dysbiosis, should be considered. Antibiotics are powerful agents inducing dysbiosis and the authors wanted to explore the possible relationship between the consumption of different major classes of antibiotics and the prevalence of diabetes (type-1, /T1D/, type-2 /T2D/) in thirty European countries. According to our hypothesis, if such association exists, the dominant use of certain major antibiotic classes might be reflected in the prevalence of T1D and T2D in different countries. Comparisons were performed between the prevalence of diabetes (T1D and T2D) estimated for 2019 and featured in the Diabetes Atlas with the average yearly consumption of major antibiotic classes of the previous 10 years (2010-19) extracted from the ECDC yearly reports on antibiotic consumption in Europe. Pearson correlation and variance analysis were used to estimate the possible relationship. Strong, positive (enhancer) associations were found between the prevalence of T1D and the consumption of tetracycline (J01A /p: 0.001/) and the narrow spectrum penicillin (J01CE /p: 0,006/, CF /p: 0.018/). Strong negative (inhibitor) association was observed with broad-spectrum, beta-lactamase resistant penicillin (J01CR /p: 0.003/), macrolide (J01F /p: 0.008/) and quinolone (J01M /p: 0.001/). T2D showed significant positive associations with cephalosporin (J01D /p: 0.048/) and quinolone (J01M /p: 0.025/), and a non-significant negative association was detected with broad-spectrum, beta-lactamase-sensitive penicillin (J01CA /p: 0.67/). Countries with the highest prevalence of diabetes (first 10 positions) showed concordance with the higher consumption of “enhancer” and the lower consumption of “inhibitor” antibiotics (first 10 positions) as indicated by variance analysis. Countries with high prevalence of T1D showed high consumption of tetracycline (p: 0.015), and narrow spectrum, beta-lactamase sensitive penicillin (p: 0.008), and low consumption of “inhibitor” antibiotics (broad-spectrum, beta-lactamase resistant, combination penicillin (p: 0.005), cephalosporin (p: 0.036), and quinolone (p: 0.003). Countries with a high prevalence of T2D consumed more cephalosporin (p: 0.084), quinolone (p: 0.54), and less broad-spectrum, beta-lactamase sensitive penicillin (p: 0.012) than other countries. Conclusion/Interpretation: The development of diabetes-related dysbiosis might be attached to higher consumption of specific classes of antibiotics, showing positive (enhancer) associations with the prevalence of diabetes, and the low consumption of other classes of antibiotics shoving negative (inhibitory) associations. Those groups of antibiotics are different in T1D and T2D

Preprint ARTICLE | doi:10.20944/preprints202007.0064.v1

Numerical Fire Spread Simulation Based on Material Pyrolysis - An Application to the CHRISTIFIRE Phase 1 Horizontal Cable Tray Tests

Tristan Hehnen, Lukas Arnold, Saverio La Mendola

Subject: Engineering, Control And Systems Engineering Keywords: CHRISTIFIRE; Fire Dynamics Simulator (FDS); pyrolysis modelling; shuffled complex evolution (SCE); high performance computing (HPC); fire propagation simulation; cone calorimeter simulation; cable tray fire simulation; SPOTPY; PROPTI

Online: 5 July 2020 (10:55:20 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202005.0220.v1

Tuberculosis Following PD-1 Inhibitor in A Patient with Non-Small Cell Lung Cancer; A Case Report & Literature Review

Nabil Omar

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: checkpoint inhibitors; tuberculosis; non-small cell lung cancer (NSCLC); Anti-PD-1; toxicity management; immunotherapy; pembrolizumab

Online: 13 May 2020 (04:38:50 CEST)

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Immune checkpoint inhibitors (ICIs) – anti-programmed death-1 (PD-1) and their ligands (PD-L1 and PD-L2) have become widely used in the treatment of several malignancies. Many immune-related adverse events have been linked to these agents. However, tuberculosis (TB) reactivation during their use is increasingly reported. Herein, we present a 58-year-old lady with advanced non-small cell lung cancer (NSCLC) ALK-negative, EGFR wild, and PD-L1 Immune histochemistry (IHC) strongly positive in 95% of tumor cells. The patient presented with high-grade fever and a history of productive cough for a 1-week duration. A few days later, she was diagnosed with pulmonary tuberculosis following the 6th cycle of Pembrolizumab, an anti-PD-1 monoclonal antibody. AFB smear and TB PCR from BAL were positive (rifampin resistance not detected), and she was accordingly started on Anti-TB medications. Immunotherapy was held. Of note, the patient had a history of sick contact with a patient with active TB infection ten years ago, but there was no documentation of latent TB or previous TB infection. Her HIV status is negative. Her sputum AFB smear continued to be positive after four weeks of anti-TB medications. Later, the patient was discharged after her sputum was cleared from AFB (negative x 2 sets). We assumed that our patient developed reactivation of pulmonary tuberculosis secondary to an immune checkpoint inhibitor (Pembrolizumab). She was not re-challenged with Pembrolizumab following TB diagnosis. To our knowledge, this is the first reported case from the Arab and the Middle East; it reinforces the previous observations of the association between ICIs administration and the development of MTB. Nevertheless, further studies in the clinical setting are necessary to establish the exact mechanism involved in this association. Oncologists' awareness & prompt recognition of this potential hazardous consequence are essential. Since there is no clear evidence whether LTBT prior PD-1/PD targeted immunotherapy is required, targeted LTBT before starting ICIs immunotherapy with TB chemoprophylaxis; yet to be explored, particularly in the regions where the MTB prevalence is high.

Preprint ARTICLE | doi:10.20944/preprints202001.0255.v1

Cordycepin Resensitizes T24R2, Cisplatin-resistant Human Bladder Cancer Cell, to Cisplatin by Inhibiting the AKT-mediated Ets-1 Activation

Kwang dong Kim, Sang-Seok Oh, Ki Won Lee, Jin-Woo Jeong, Soojong Park, Minju Kim, Yerin Lee, Hyun-Tak Han, Cheol Hwangbo, Jiyun Yoo

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cordycepin; Cisplatin-resistance; Resensitization; MDR1; Ets-1; AKT

Online: 22 January 2020 (09:18:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints201812.0293.v1

Diastereomeric Recognition of 5',8-cyclo-2'-deoxyadenosine Lesions by Human poly(ADP-ribose) Polymerase 1 in Biomimetic Model

Annalisa Masi, Arianna Sabbia, Carla Ferreri, Francesco Manoli, Yanhao Lai, Eduardo Laverde, Yuan Liu, Marios G. Krokidis, Chryssostomos Chatgilialoglu, Maria Rosaria Faraone Mennella

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: human poly(ADP-ribose) polymerase 1 (PARP1), PARP-DNA complex,DNA-protein binding,DNA repair, 5′,8-Cyclopurine-2′-deoxynucleoside, DNA damage , DNA repair efficiency.

Online: 24 December 2018 (16:01:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints201804.0057.v1

Auto-Extraction of Linear Archaeological Traces of Tuntian Irrigation Canals in Miran Site (China) from Gaofen-1 Satellite Imagery

Lei Luo, Xinyuan Wang, Rosa Lasaponara, Bo Xiang, Jing Zhen, Lanwei Zhu, Ruixia Yang, Decheng Liu, Chuansheng Liu

Subject: Arts And Humanities, Archaeology Keywords: auto-extraction; remote sensing archaeology; tuntian; LATTICs; GF-1; Silk Road; Miran

Online: 4 April 2018 (11:56:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201710.0159.v1

Photodegradation of 1-butyl-3-methylimidazolium chloride [bmim]Cl via Synergistic Effect of Adsorption-Photodegradation of Fe-TiO₂/AC

Azhar Zawawi, Raihan Mahirah Ramli, Noorfidza Yub Harun

Subject: Engineering, Chemical Engineering Keywords: photodegradation; TiO2; ionic liquids; activated carbon; synergistic effect

Online: 24 October 2017 (11:32:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0279.v1

In Vitro Combinatorial Activity of Direct Acting Antivirals and Monoclonal Antibodies against the Ancestral B.1 and BQ.1.1 SARS-CoV-2 Viral Variants

Lia Fiaschi, Camilla Biba, Ilenia Varasi, Niccolò Bartolini, Chiara Paletti, Federica Giammarino, Francesco Saladini, Maurizio Zazzi, Ilaria Vicenti

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; mAbs; DAA; Combination antiviral therapy; In vitro synergism; Omicron variant

Online: 3 January 2024 (19:28:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202410.0416.v1

Tumor Associated Macrophages (TAMs)-Related Cytokines, sCD163, CCL2 and CCL4, as Novel Biomarkers for Overall Survival and Time to Treatment in Waldenstrom’s Macroglobulinemia, Emphasis on Asymptomatic WM

Alexandros Gkiokas, Mavra Papadatou-Gigante, Annita Ioanna Gkioka, Aspasia Koudouna, Thomai M. Tryfou, Alexandros Alexandros Alexandropoulos, Vassiliki Bartzi, Nikolitsa Kafasi, Marie-Christine Kyrtsonis

Subject: Medicine And Pharmacology, Hematology Keywords: soluble CD163 (sCD163); CCL2 {Monocyte Chemoattractant Protein-1 (MCP-1)}; CCL4 {Macrophage Inflammatory Protein 1 beta (MIP-1b)}; Tumor-associated macrophages (TAMs); Waldenstrom’ s Macroglobulinemia (WM); Asymptomatic Waldenstrom’ s Macroglobulinemia (AWM); biomarkers; prognosis ;

Online: 7 October 2024 (08:00:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1849.v1

Analysis of Cow Milk Fatty Acid and Protein Composition in Different Breeds and Regions

Yunxia Zou, Yifei Chen, Qingyong Meng, Yachun Wang, Yali Zhang

Subject: Biology And Life Sciences, Other Keywords: bovine milk; breeds; fatty acids; protein composition; region

Online: 24 September 2024 (04:49:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0023.v1

An In-silico Designing of Vaccine against All Serotypes of Dengue Virus Based on Virtual Screening of B-Cell and T-Cell Epitopes

Hikmat Ullah, Shaukat Ullah, Li Jinze, Fan Yang, Lei Tan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Dengue virus; in-silico designing; B cell epitope; T cell Epitopes; pan-serotype vaccine; molecular docking

Online: 1 July 2024 (09:12:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1184.v1

Prognostic value of platelet–lymphocyte ratio, neutrophil–lymphocyte ratio and monocyte–lymphocyte ratio in HNSCC - a retrospective single center study

Camil Ciprian Mirestean, MIHAI COSMIN STAN, Roxana Irina Iancu, Dragoș Petru Teodor Iancu, FLORINEL BADULESCU

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: head and neck cancers, HNC, HNSCC, NLR, PLR, MLR, biomarkers, prognostic

Online: 18 July 2023 (07:38:43 CEST)

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Introduction: The identification of some prognostic markers in head and neck cancers (HNC) is necessary for the stratification of the therapeutic approach with benefits in increasing treatment response rates and limiting the toxic side effects of treatments. Neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and pallets-to-lymphocyte ratio (PLR) are currently validated as cheap and accessible biomarkers in different types of solid tumors. The purpose of the study was to evaluate the possible biomarker value of NLR, PLR and MLR recorded pre-treatment (radiotherapy/chemotherapy) for prognostic purposes in HNC. Materials and methods: The study included 190 patients with HNC included in the oncology record in the oncology outpatient clinic of the Craiova County Emergency Hospital starting from January 2002 and deceased until December 2022. Among them, 40 patients treated multimodally in the Clinic were included in the study of Oncology of the Craiova County Emergency Hospital for which the pre-treatment value (chemotherapy/radiotherapy) of NLR, PLR and MLR could be calculated. Overall survival (OS) values were correlated with NLR, PLR and MLR. Results: Median values for NLR, PLR and MLR were 6.15 (1.24-69), 200.79 (61.3- 1775.0) and 0.53 (0.12-5.5) respectively. In the study, the mean values for NLR, PLR and MLR of 2.88, 142.97 and 0.36 respectively were obtained. Median OS in the study group was 11 months (1-120). Although a negative Pearson`s correlation, the relationship between your variables was only weak, values R =.07, p=.67; R=.02, p=.31 and R=07, p=.62 being related with NLR, PLR and MLR respectively correlation with overall survival (OS). The median values of NLR, PLR and MLR were calculated (1.53, 90.32 and 0.18 respectively) for HNC cases with pre-treatment values of NLR <2 and for HNC cases with NLR values ≥6 (23.5, 232.78 and 0.79 respectively). Median OS for cases with NLR<2 and NLR≥6 were 17.4 and 13 months, respectively. Conclusions: The negative correlations of NLR, PLR and MLR with OS reported in the study are in accordance with the data reported in the literature for locally advanced recurrent and metastatic HNSCC cases. The comparative analysis of the data in the group with NLR<2 and NLR≥6 highlights an advantage of 4.4 months in median OS in favor of the group with low values of NLR. Being cheap and accessible, these markers could change the therapeutic approach even in centers with limited resources. The role of borderline NLR values as a prognostic factor in HNSCC must also be defined. PLR and MLR are less evaluated as biomarkers but the study demonstrates their potential to be used as prognostic biomarkers, it remains to be clarified if their inclusion in multivariable models along with NLR would bring a benefit

Preprint REVIEW | doi:10.20944/preprints202405.0005.v1

Precision Fermentation as an Alternative to Animal Protein, a Review

Marilia M. Knychala, Larissa A. Boing, Jaciane L. Ienczak, Débora Trichez, Boris U. Stambuk

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Precision fermentation; recombinant casein; recombinant whey proteins; recombinant egg-white proteins; recombinant collagen; recombinant hemoglobin; recombinant myoglobin; recombinant albumin; cellular agriculture

Online: 1 May 2024 (07:39:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.0035.v1

Soluble PD-L1 and PD-1 Significantly Improve the Accuracy of a Diagnostic Model of mRNA Transcripts in the Diagnosis of Prostate Cancer

Margarita Žvirblė, Ieva Vaicekauskaitė, Žilvinas Survila, Paulius Bosas, Neringa Dobrovolskienė, Agata Mlynska, Rasa Sabaliauskaitė, Vita Pašukonienė

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: prostate cancer; sPD-L1; sPD-1; androgen receptor; mRNA transcripts

Online: 1 October 2024 (14:32:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2444.v1

Comparing a Gauge-Invariant Formulation and a “Conventional Complete Gauge-Fixing Approach” for l = 0, 1 Mode Perturbations on the Schwarzschild Background Spacetime

Kouji Nakamura

Subject: Physical Sciences, Theoretical Physics Keywords: black hole; Schwarzschild spacetime; perturbation theory; gauge-invariance; gauge-fixing

Online: 30 September 2024 (15:26:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0776.v1

Interleukin-1ß / Interleukin (IL)-1-Receptor-Antagonist (IL1-RA) Axis in Invasive Bladder Cancer – An Exploratory Analysis of Clinical and Tumor Biological Significance

Marko Vukevic, Jorge M Chamlati, Jörg Hennenlotter, Tilman Todenhöfer, Thomas Lütfrenk, Sebastian Jersinovic, Igor Tsaur, Arnulf Stenzl, Steffen Rausch¹

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: urothelial cancer; metastasis; prognosis; biomarker; pathway; autophagy

Online: 10 January 2024 (08:05:05 CET)

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Background: Previous data indicate a role of IL-1 and IL-1RA imbalance in bladder carcinoma (BC) and inhibition of IL-1 signaling might be considered a treatment option. Objective: To assess expression patterns and the prognostic role of IL-1ß and IL-1RA in invasive BC and to evaluate their interaction with AKT signaling and proliferation. Design, Setting, and Participants: The study included two independent cohorts of n=92 and n=102 patients who underwent radical cystectomy for BC. Specimen from BC and benign urothelium (n=22 and n=39) were processed to a tissue microarray and immunohistochemically stained for IL-1ß, IL-1RA, AKT and Ki-67. Expression scores were correlated to clinical variables and Ki-67 and AKT expression. Outcome measurements and statistical analysis: Association with outcome was assessed using Wilcoxon Kruskal-Wallis tests, Chi-square tests or linear regression, dependent on the variable’s category. Kaplan–Meier and Cox proportional hazard analyses were used to estimate recurrence-free (RFS), cancer-specific (CSS) and overall survival (OS). Results: Both, IL-1ß and IL-1RA were significantly overexpressed in invasive BC compared to benign urothelium in both cohorts (p<0.005). IL-1ß was associated with vascular invasion (210 vs. 183, p<0.02), lymphatic invasion (210 vs. 180, <0.05) and G3 cancer (192 vs. 188, <0.04). Survival analysis revealed favorable RFS, CSS, and OS in case of high IL-1ß expression (p<0.02, <0.03 and <0.006, respectively). Multivariate analyses revealed an independent impact of (low) IL1ß expression on RFS, CSS and OS. IL-1ß and IL-1ß/IL-1RA ratio were positively correlated to AKT expression (p<0.05 and <0.01, respectively). Additionally, high expression of Ki-67 (>15%) correlated with higher levels of IL-1β (p=0.01). Conclusions: Overexpression of IL-1ß and IL-1RA is frequently found in BC, with a prognostic significance observed for IL-1ß protein expression. The observed link between the IL-1ß / IL-1RA axis and AKT signaling may indicate possible autophagy activation processes beside the known tumor-promoting effects of AKT.

Preprint REVIEW | doi:10.20944/preprints202311.1049.v1

New Die-Compaction Equations for Powders as a Result of Known Equations’ Correction. Part 1. Review and Analysis of Various Die-Compaction Equations

Anatolii Laptiev

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: powders; pressure; density; die-compaction equation; approximation.

Online: 16 November 2023 (11:20:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0532.v1

Tumor-Infiltrating iNKT Cells Activated through C-Kit/Sca-1 Are Induced by Pentoxifylline, Norcantharidin, and Their Mixtures for Killing murine Melanoma Cells

Maximiliano V.M Correa-Lara, Israel Lara-Vega, Minerva Nájera-Martínez, María Lilia Domínguez-López, Elba Reyes-Maldonado, Armando Vega-López

Subject: Biology And Life Sciences, Toxicology Keywords: NK; iNKT; CD107a+; c+kit+; Lya6A+

Online: 7 September 2023 (10:59:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0138.v1

Approaching High-Performance TS-1 Zeolites in the Presence of Alkali Metal Ions via Combination of Adjusting pH Value and Modulating Crystal Size

Geng Li, Kairui Fu, Fulin Xu, Tianduo Li, Yunan Wang, Jingui Wang

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: Zeolites; TS-1; Inorganic alkali; Titanium; Lewis acid; Oxidation

Online: 4 July 2023 (08:13:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1300.v1

3EE, 20Ac-Ingenol Decreases HIF-1α/PD-L1 through ATR Activation under Hypoxia and Induces Apoptosis in Panc-1 Cells Accumulating Cyclin D1

Shohei Miyata, Takashi Ishii, Susumu Kitanaka

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Apoptosis; PD-L1; catalytic topo I inhibitor; cyclin D1 overexpression; ATR; p53

Online: 18 May 2023 (08:31:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0189.v1

Determination of the Binding Epitope of an Anti-mouse CCR9 Monoclonal Antibody (C9Mab-24) using the 1 × alanine and 2 × alanine-substitution Method

Hiyori Kobayashi, Teizo Asano, Tomohiro Tanaka, Hiroyuki Suzuki, Mika K. Kaneko, Yukinari Kato

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mouse CCR9, monoclonal antibody, epitope mapping, alanine scanning, enzyme-linked immunosorbent assay

Online: 12 December 2022 (03:54:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0292.v1

Identification of Polymorphism Angiotensin Ii Type 1 Receptor (AT1) 1166 A/C Gene and Potassium Levels in Pre-Eclampsia among The Madurese Population

Zakkiyatus Zainiyah, Eny Susanti, Novita Wulandari

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: Preeclampsi; AT1; Pottasium; preeclampsi; Madurese

Online: 16 November 2022 (01:47:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0038.v3

Application of Local Gauge Theories to Fluid Mechanics Part 1 - Fluid Mechanics as a Local Gauge Theory and Relation to Navier-Stokes Equations

Thomas Merz

Subject: Physical Sciences, Condensed Matter Physics Keywords: fluid dynamics; turbulent flow; stationary flow; SO(3) local gauge; quasiparticle; non-linearity

Online: 12 October 2022 (12:23:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0334.v1

Computational Biology and Machine Learning Approaches to Identify the Rubber Tree (Hevea brasiliensis L.) Genome Encoded Potential MicroRNAs Targeting Rubber Tree Virus 1

Muhammad Aleem Ashraf, Hafiza Kashaf Tariq, Jallat Khan, Han Cheng

Subject: Biology And Life Sciences, Plant Sciences Keywords: Rubber tree capillovirus 1; microRNAs; plant-virus interaction; RNAi: computational algorithms; gene silencing, minimum free energy

Online: 22 July 2022 (09:52:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0498.v1

Pretreatment Serum Levels of IL-1 Receptor Antagonist and IL-4 are Predictors of Overall Survival in Multiple Myeloma Patients Treated with Bortezomib

Damian Mikulski, Paweł Robak, Ewelina Perdas, Edyta Węgłowska, Aleksandra Łosiewicz, Izabela Dróżdż, Dariusz Jarych, Małgorzata Misiewicz, Janusz Szemraj, Wojciech Fendler, Tadeusz Robak

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: bortezomib; IL-13; IL-1Ra; IL-4; multiple myeloma; OS; PFS

Online: 26 November 2021 (10:31:50 CET)

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Multiple myeloma (MM) is characterized by the malignant proliferation of monoclonal plasma cells in the bone marrow with an elevation in monoclonal paraprotein, renal impairment, hypercalcemia, lytic bony lesions, and anemia. Immune cells and associated cytokines play a significant role in MM growth, progression, and dissemination. While some cytokines and their clinical significance are well described in MM biology, others remain relatively unknown. The aim of the present study was to assess the impact of pretreatment serum levels of 27 selected cytokines on progression-free survival (PFS) and overall survival (OS) in MM patients before first-line therapy with bortezomib-based regimens. Serum cytokine levels were assayed with a Bio-Rad Bio-Plex Pro Human Cytokine 27-Plex Assay on the MAGPIX Multiplex Reader and the Bio-Plex® 200 System (Bio-Rad) including IL-1β, IL-1Ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Eotaxin, FGF, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α, and VEGF. A total of 61 MM patients were examined. Most patients received a bortezomib, cyclophosphamide and dexamethasone (VCD) chemotherapy regimen. In the final multivariate model, IL-13 cytokine level (HR 0.1411, 95% CI: 0.0240-0.8291, p = 0.0302), and ASCT (HR 0.3722, 95% CI: 0.1826-0.7585, p=0.0065) significantly impacted PFS. Furthermore, ASCT (HR 0.142, 95% CI: 0.046-0.438, p=0.0007), presence of bone disease at diagnosis (HR 3.826, 95% CI: 1.471-9.949, p=0.0059) and two cytokine levels- IL-1Ra (HR 1.017, 95% CI: 1.004-1.030, p= 0.0091) and IL-4 (HR 0.161, 95% CI: 0.037-0.698, p = 0.0147) were independent predictors of OS. Three clusters of MM patients were identified with different cytokine profiles. In conclusion, serum pretreatment levels of IL-13 and IL-4 are predictors of better PFS and OS, respectively, whereas IL-1Ra pretreatment levels negatively impact OS in MM patients treated with bortezomib-based chemotherapy. Cytokine signature profile may have a potential influence on the outcome of patients treated with bortezomib.

Preprint ARTICLE | doi:10.20944/preprints202109.0375.v1

The Pseudo-Symmetric N-benzyl Hydroxyethylamine Core in a New Series of Heteroarylcarboxyamide HIV-1 Pr Inhibitors: Synthesis, Molecular Modeling and Biological Evaluation

Rosarita D'Orsi, Maria Funicello, Paolo Lupattelli, Federico Berti, Lucia Chiummiento

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: HIV-protease inhibitors; pseudo-symmetric core; heteroaryl carboxyamides; synthesis; biological screening; molecular modeling

Online: 22 September 2021 (10:44:16 CEST)

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