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Preprint ARTICLE | doi:10.20944/preprints202405.0676.v1

Comparison of In Vitro Models for Drug-Induced Liver Injury Assessment

Nam-Ju Kim, Ji-Hyun Bang, Hee Yi, Hyun-Ok Ku, Joong-Sum Kim, Seon-Jong Yun, Byung-Suk Jeon

Subject: Biology And Life Sciences, Toxicology Keywords: 2D and 3D cultures; HepG2; HepaRG; liver injury markers; CYP450

Online: 10 May 2024 (10:42:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1963.v1

HP-YOLOv8: High-Precision Small Object Detection Algorithm for Remote Sensing Images

Guangzhen Yao, Sandong Zhu, Long Zhang, And Miao Qi

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: YOLOv8; Small Object Detection; Remote Sensing Images; attention mechanism; feature 21
fusion

Online: 27 June 2024 (18:44:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1468.v1

Grapevine Trunk Diseases in Greece: Disease Incidence and Fungi Involved in Discrete Geographical Zones and Varieties

Stefanos I. Testempasis, Emmanouil A. Markakis, Georgia I. Tavlaki, Stefanos K. Soultatos, Christos Tsoukas, Danai Gkizi, Aliki K. Tzima, Epameinondas Paplomatas, Georgios S. Karaoglanidis

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Kalmusia variispora; Neosetophoma italica; Paraconiothyrium variabile; Seimatosporium vitis; meteorological conditions; wood inhabitant

Online: 23 November 2023 (05:42:59 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202404.0136.v1

From Investigating a Case of Cellulitis to Exploring Nosocomial Infection Control of ST1 Legionella pneumophila Using Genomic Approaches

Charlotte Michel, Fedoua Echahidi, Sammy Place, Lorenzo Filippin, Vincent Colombie, Nicolas Yin, Delphine Martiny, Olivier Vandenberg, Denis Piérard, Marie Hallin

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Legionella pneumophila; Legionella pneumophila ST1; WGS; genomic typing; wgMLST; cgMLST; wgSNP; nosocomial; hygiene investigation

Online: 2 April 2024 (07:26:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0476.v1

Metabolomics on Apple (Malus domestica) Cuticle—Search for Authenticity Markers

Kamila Bechynska, Jiri Sedlak, Leos Uttl, Vit Kosek, Petra Vackova, Vladimir Kocourek, Jana Hajslova

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Malus domestica; apple cuticle; UHPLC-HRMS/MS; metabolomic fingerprints; classification models; markers; wax esters

Online: 8 April 2024 (03:04:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.2052.v1

The Info-Geometric Analysis of Wind Speed Dynamics and How Energy Is Influentially Impacted by Information Geometry (IG)

Dr Ismail A Mageed

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Wind Speed Manifold (WSM), IG,GeodesicEquations (GEs)

Online: 30 January 2024 (08:40:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0184.v1

Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches

Peter James Icalia Gann, Jimmbeth Zenila P. Fabia, Althea Gay B. Pagurayan, Ma. Joy Theresa Agcaoili, Ryan James J. Pascual, Suerte M. Baranda, Arc Josam J. Racho, Marvielyn P. Olivar, Jayson F. Cariaga, Alvin Domingo, Dionisio S. Bucao, Shirley C. Agrupis

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: probiotics; nutrient digestion; antibiotic resistance; antibacterial activity; whole genome sequence

Online: 2 April 2024 (10:16:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1672.v1

Phenolic Monoterpenes Conversion of Conobea scoparioides Essential Oil by Hydrotalcite Synthesized from Blast-Furnace Slag

Monaliza M. Rebelo, Eloisa Helena A. Andrade, Jose Augusto M. Corrêa, José Guilherme S. Maia

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: pataqueira; thymol; essential oil composition; hydrotalcite; blast furnace slag

Online: 28 March 2024 (04:27:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1703.v1

A Single Dose Intraperitoneal Injection of P. gingivalis Outer Membrane Vesicle (OMV) Stimulated Expressions of Neuroinflammatory Markers and Histopathological Changes in the Brain of Adult Zebrafish

Malik Adewoyin, Hamarsha Mohamed, Rasaq Akinsola, Seong Lin Teoh, Mohammad Noor Amall Azmai, Noraini Abu Bakar, Nurrul Shaqinah Nasruddin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: P. gingivalis; Outer membrane vesicles; Neuroinflammation

Online: 25 August 2024 (06:38:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0808.v1

Gut Microbiome and Metabolome Alterations in Overweight or Obese Adult Population after Weight-Loss Bifidobacterium brevis BBr60 Intervention: A Randomized Controlled Trial

Zhouya Bai, Ying Wu, Dejiao Gao, Yao Dong, Yujia Pan, Shaobin Gu

Subject: Biology And Life Sciences, Life Sciences Keywords: Bifidobacterium brevis BBr60; overweight or obese; randomized controlled trial; gut microbiota; metabolic profile

Online: 10 September 2024 (13:38:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1155.v1

Antimicrobial Effects of Antibiotics in Combination with Oregano Essential Oil against Staphylococcus aureus

Neli Ermenlieva, Gabriela Tsankova, Gergana Nedelcheva, Sylvia Stamova, Krasimira Laleva, Emiliya Georgieva

Subject: Medicine And Pharmacology, Other Keywords: antimicrobial effects; antibiotics; oregano essential oil; combination; S. aureus

Online: 16 August 2024 (03:31:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1447.v1

The Effects of Egg- and Substrate-Associated Microbiota on the Larval Performance of the Housefly, Musca domestica

Rasmus Majland Dyrholm, Pernille Arent Simonsen, Cino Pertoldi, Toke Munk Schou, Asmus Toftkær Muurmann, Simon Bahrndorff

Subject: Biology And Life Sciences, Insect Science Keywords: Musca domestica; insect production; larval performance; microorganisms; egg disinfection; substrate autoclaving

Online: 18 July 2024 (08:54:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1888.v1

Molecular Mechanism and Signal Transduction of Resistance to Low-Temperature Stress Induced by Bacillus amyloliquefaciens GL18 in Avena sativa

Lan Chen, Tian Wang, Ying Zhang, Yongli Xie, Feifei Chang, Lingling Wu, Ying Gao, Junxi Li

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: low-temperature stress; Bacillus amyloliquefaciens; ‘ Qingyan 1’ ; transcriptome; signal transduction pathway analysis

Online: 27 August 2024 (15:34:47 CEST)

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Bacillus amyloliquefaciens GL18 has been proven to have good resistance ability and can promote the growth of Avena sativa cultivar ‘Qingyan 1’. Herein, Illumina high-throughput transcriptome sequencing technology was used to analyze the differential expression of genes between the interaction group (LB) and the control group (LCK) when ‘Qingyan 1’ roots interacted with GL18 bacterial suspension or sterile water for 12 h under 4 °C low-temperature stress. The gene function annotation information was obtained using Gene Ontology (GO) functional classification and enrichment analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and annotation of differentially expressed genes was used to explore the related signal transduction pathway of ‘Qingyan 1’ response to cold stress induced by Bacillus GL18. The results showed that after ‘Qingyan 1’ seedlings interacted with GL18 bacterial suspension or sterile water for 12 h, comparing the LB with the LCK or CK (the control group at 26 °C), there were 12,061 and 25,489 differentially expressed genes, respectively. Their functions were mainly related to metabolic process, cellular process, catalytic activity, binding, and cellular parts, and they were significantly enriched in plant tissue growth and development, cell wall, organelle membrane synthesis, thiamine and its compounds, biosynthesis, polyamine decomposition, and other metabolic processes. Signal transduction was detected through KEGG pathway enrichment analysis, and corresponding genes, such as AUXI, PP2C, SNRK2, ABF, BRI1, and PIF4, were expressed. The expression of genes directly involved in plant growth was upregulated in the presence of GL18, as shown by real-time quantitative PCR analysis. It was speculated that the normal growth and development of ‘Qingyan 1’ under low-temperature stress promoted by GL18 is a result of the integration of multiple pathways in terms of regulating endogenous hormone metabolism, secondary metabolite synthesis, and photosynthesis.

Preprint ARTICLE | doi:10.20944/preprints202403.0023.v1

Co-occurrence of Borrelia burgdorferi sensu lato and Babesia spp. DNA in Ixodes ricinus Ticks Collected from Vegetation and Pets in the City of Poznań, Poland

Justyna Liberska, Jerzy Michalik, Julia Olechnowicz, Miroslawa Dabert

Subject: Biology And Life Sciences, Parasitology Keywords: Ixodes ricinus; Borrelia burgdorferi s.l.; Babesia microti; Babesia venatorum; Babesia canis; Borrelia miyamotoi; coinfections, babesiosis, urban green areas

Online: 1 March 2024 (05:42:09 CET)

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Green spaces localized in cities create favourable environmental conditions for Ixodes ricinus, the most widespread and important vector of tick-borne pathogens in Central Europe. In this study, we described the prevalence of Borrelia burgdorferi s.l. and Babesia species found in mono- and double infections among I. ricinus ticks occurring in urban areas of the city of Poznań, west-central Poland. Two tick groups were examined: (i) a group of 1,029 host-seeking ticks (460 nymphs, 289 females, and 280 males and (ii) a group of 1,268 engorged female ticks removed from 1,115 tick-infested animals, dogs and cats. The overall prevalence of B. burgdorferi s.l. found in host-seeking ticks was almost two-fold higher in comparison to ticks derived from pets (8.7 vs. 4.7%, respectively, χ2 test, P = 0.001). Borrelia afzelii and B. garinii were the most prevalent species both in ticks from vegetation (3.7% and 3.7%, respectively) and from pets (3.7% and 0.6%, respectively). Babesia infections were slightly more common in feeding ticks compared to those from vegetation (6.0 vs. 4.7%, respectively), however, the difference was not significant. Three Babesia species including Ba. microti, Ba. venatorum and Ba. canis were identified. Babesia canis and Ba. microti were the most prevalent in host-seeking (2.6% and 1.4%, respectively) and feeding ticks (2.8% and 2.2%, respectively), whereas Ba. venatorum was the rarest piroplasm. All Ba microti obtained sequences (n=42) were identical to Ba. microti genotype "Jena/Germany" that is considered as pathogenic to humans. Furthermore, the sequences of Ba. venatorum (n=17) were identical with those isolated from patients in Italy, Austria and Poland. Altogether, out of 121 Babesia-positive ticks, 19.8% were simultaneously infected with B. burgdorferi s.l. spirochetes. We found similar prevalences of co-infections in host-seeking (1.1%) as well as in feeding ticks (1.0%). In the first group, Ba. canis prevailed in coinfections (73%), mostly with B. afzelii and B. garinii, in the second group, Babesia microti dominated with B. afzelii (92.7%). This is the first finding documenting the co-occurrence of both pathogens in ticks from companion animals. The detection of Ba. microti and Ba. venatorum sequences identical to strains pathogenic to humans as well as predominance of LB spirochetes, B. afzelii and B. garinii in I. ricinus from vegetation and animals indicates that there is a potential risk of acquiring human babesiosis and/or Lyme borreliosis in urban areas of the city of Poznań. Although, the overall risk of encountering a Ba. microti and B. burgdorferi s.l. co-infected tick was low (1.0%), the potential for more severe clinical symptoms and complications deserves notice. A relatively high infection rates of Ba. canis in dog-derived ticks (3.2%) and in host-seeking ticks (2.6%), suggests that I. ricinus could potentially be involved in the circulation of this piroplasm in areas, where the competent vector, Dermacentor reticulatus is absent or rare.

Preprint ARTICLE | doi:10.20944/preprints202404.0446.v1

Exploring the Relationship between Melioidosis Morbidity Rate and Local Environmental Indicators Using Remotely Sensed Data

Jaruwan Wongbutdee, Jutharat Jittimanee, Suwaporn Dandee, Pongthep Thongsang, Wacharapong Saengnill

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Geographically Weighted Poisson Regression; Google earth engine; Spatial model; Burkholderia pseudomallei

Online: 5 April 2024 (14:21:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0859.v1

Development of a Whole-Cell System Based on Genetically Modified Protoplasts to Detect Nickel Ions in Food Matrices

Monica De Caroli, Carla Perrotta, Patrizia Rampino

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: whole-cell system; Nicotiana tabacum protoplasts; HSP promoter; heavy metals; food safety

Online: 13 May 2024 (13:06:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1304.v1

Alternaria alternata and A.koreana, the Causal Agent of a New Leaf Spot in Celtis sinensis and Their Sensitivity to Fungicides

Qiuqin Wang, Xiu-Yu Zhang, Yu Wan, Yin-Juan Zhao

Subject: Environmental And Earth Sciences, Other Keywords: Celtis sinensis; Alternaria alternata; Alternaria koreana; fungi; identification; fungicides

Online: 21 November 2023 (09:39:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0730.v1

Cloning and Mutagenesis of ampC Encoding for β–lactamase in Pseudomonas aeruginosa to Identify Potential Allosteric Binding Sites on β–Lactamase

Shriya Bhat

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Pseudomonas aeruginos; &beta; – lactamase; ampC

Online: 12 May 2024 (07:44:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1754.v1

Potential of Using Entomopathogenic Fungi for Controlling Colorado Potato Beetle (Leptinotarsa decemlineata Say.) Populations Under Climate Change

Rauza Abdukerim, Meiramgul Mussina, Gaukartas Abysheva, Nagima Tumenbayeva, Bekzat Mombayeva, Assel Karabayeva, Nurgul Amangeldi, Zamzagul Amangeldikyzy

Subject: Biology And Life Sciences, Insect Science Keywords: Colorado potato beetle; climate change; biological plant protection; entomopathogenic fungi; virulence; thermotolerance; B. bassiana; B. pseudobassiana

Online: 23 September 2024 (10:52:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0371.v1

Is Entropy a Force of Nature?

Martin Johnson

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: dark energy
cosmic background radiation
dark ages
large-scale structure of the Universe

Online: 7 February 2024 (03:37:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.2010.v1

Ciprofloxacin Concentrations in Food Could Select for Quinolone Resistance in Enterobacteriaceae: An In Vivo Study in Galleria mellonella

Nele Panis, Zina Gestels, Dorien Van Den Bossche, Irith De Baetselier, Said Abdellati, Thibaut Vanbaelen, Tessa de Block, Sheeba Santhini Manoharan-Basil, Chris Kenyon

Subject: Biology And Life Sciences, Life Sciences Keywords: K. pneumoniae; ciprofloxacin; AMR; MSC (minimal selective concentration); G. mellonella; ADI (acceptable daily intake)

Online: 25 September 2024 (11:56:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0223.v3

Metabolic Modeling Elucidates Phenformin and Atpenin A5 as Broad-Spectrum Antiviral Drugs

Alina Renz, Mirjam Hohner, Maximilian Breitenbach, Jonathan Josephs-Spaulding, Johanna Dürrwald, Lena Best, Victoria Dulière, Chloé Mialon, Stefanie M. Bader, Raphaël Jami, Georgios Marinos, Nantia Leonidou, Filipe Cabreiro, Marc Pellegrini, Marcel Doerflinger, Manuel Rosa-Calatrava, Andrés Pizzorno, Andreas Dräger, Michael Schindler, Christoph Kaleta

Subject: Biology And Life Sciences, Virology Keywords: Drug discovery; Systems biology; SARS-CoV-2; Dengue; Influenza A; RSV; cell viability analysis; antivirals; in vitro validation; in vivo validation

Online: 22 March 2024 (16:46:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202408.1444.v1

Screening and Engineering Yeast Transporters to Improve Cellobiose Fermentation by Recombinant Saccharomyces cerevisiae

Leonardo G. Kretzer, Marilia M. Knychala, Lucca C. da Silva, Isadora C. C. da Fontoura, Maria José Leandro, César Fonseca, Kevin J. Verstrepen, Boris U. Stambuk

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: cellobiose; transporter; β -glucosidase; Spathaspora passalidarum; Meyerozyma guilliermondii; recombinant Saccharomyces cerevisiae

Online: 20 August 2024 (13:47:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0555.v1

Antifungal and Antibacterial Activity of Lathyrus Aphaca L. Mediated Green Synthesized CdS Nanoparticles and their Characterization

Naveed Akhtar, Himayat Ullah, Muhammad Akif, Istikhar Khan, Fawad Ali, Numan Zada Khan Mohmand

Subject: Chemistry And Materials Science, Biomaterials Keywords: Green Synthesis; CdS Nanoparticles; Lathyrus aphaca ; L.; Biological activity; Antibacterial; Antifungal

Online: 11 March 2024 (09:02:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1151.v1

Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China

Yafang Jin, Yi Li, Shaojie Huang, Chengji Hong, Xucong Feng, Huidi Cai, Yanmei Xia, Shengkai Li, Leyi Zhang, Yongliang Lou, Wanchun Guan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Salmonella; antimicrobial resistance; pathogenicity islands; cgMLST; Whole genome sequencing

Online: 16 September 2024 (03:20:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1927.v1

Prolonged CBD Pressure Mediates Higher Expression of flgA in Salmonella typhimurium

Iddrisu Ibrahim, Alexa Mathias, Daron Johnson, Emmanuel Ndezure, Joseph Ayariga, Junhuan Xu, Boakai Robertson, Olufemi Ajayi

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Salmonella Typhimurium; Cannabidiol (CBD); Antibiotic resistance; Bacterial motility; Environmental stresses; Bacterial survival

Online: 29 May 2024 (07:38:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0866.v1

Mealworm Larvae Frass Exhibits a Plant Biostimulant Effect on Lettuce, Boosting Productivity beyond Just Nutrient Release or Improved Soil Properties

Meroua Foughar, Margarida Arrobas, and Manuel Ângelo Rodrigues

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: black soldier fly frass; Hermetia illucens; Tenebrio molitor; insect droppings; manuring effect

Online: 13 June 2024 (07:36:03 CEST)

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There is a need for alternatives or complements to synthetic fertilizers to enhance agricultural sustainability. Applying organic amendments can play a significant role in this. Insect droppings show high potential, though studies evaluating their agronomic value have only recently begun to emerge. This study compared black soldier fly (Hermetia illucens) and mealworm (Tenebrio molitor) larvae frass with another organic amendment (Nutrimais) derived from composting forestry, agro-industrial, and domestic waste. The experiment also included ammonium nitrate at two rates [the same as the organic amendments, 50 kg ha–1 nitrogen (N) (FullR), and half that rate (HalfR)] and an unfertilized control. The study spanned two growth cycles of lettuce (Lactuca sativa L.) grown in pots, followed by unfertilized oats (Avena sativa L.) to assess the residual effects of the fertilizing treatments. Mealworm larvae frass mineralized rapidly, with an apparent N recovery of 37.4% over the two lettuce growth cycles, indicating its high availability to soil heterotrophic microorganisms. The average dry matter yield (DMY) of lettuce was the highest among all treatments (12.8 and 9.8 g plant–1 in the first and second lettuce cycles), even compared to the FullR treatment (12.2 and 7.8 g plant–1), though without significant differences. Although mealworm larvae frass exhibited a high mineralization rate, the DMY cannot be attributed solely to N supply, as plants in the FullR treatment showed better N nutritional status. Mealworm larvae frass provided strong evidence of a plant biostimulant effect, not explained by the variables measured in this study. Black soldier fly larvae frass exhibited typical behavior of a moderately reactive organic amendment, while Nutrimais showed low reactivity, with a near-neutral mineralization/immobilization balance. The results suggest mealworm larvae frass is recommended for early maturing vegetable crops, whereas Nutrimais appears more suitable for perennial crops with low short-term nutrient requirements.

Preprint ARTICLE | doi:10.20944/preprints202402.0222.v1

Frankincense (Boswellia serrata) Extract Effects on the Growth, Biofilm Formation, and Intracellular Infection of Porphyromonas gingivalis and Fusobacterium nucleatum in Human Gingival Epithelial Cells

David Vang, Aline Cristina de Abreu Moreira-Souza, Nicholas Zusman, German Moncada, Harmony Matshik Dakafay, Homayon Asadi, David M. Ojcius, Cassio Luiz Coutinho Almeida-da-Silva

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Porphyromonas gingivalis; Fusobacterium nucleatum; Boswellia serrata; biofilm; epithelial cells

Online: 5 February 2024 (05:37:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1477.v1

Advancing Skarn Iron Ore Detection Through Multispectral Image Fusion and 3D Convolutional Neural Networks (3D-CNN)

Jabir Abubakar, Zhaochong Zhang, Zhiguo Cheng, Fojun Yao, Abdoul-Aziz BIO SIDI D. BOUKO

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: Aster bands; Band ratio (BR) images; Principal Component (PC) bands; 3D-CNN

Online: 23 April 2024 (09:44:59 CEST)

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This study explores novel techniques to improve the detection accuracy of skarn iron deposits using advanced image processing methodologies. Leveraging the capabilities of Aster image, Band Ratio (BR) images, and Principal Component Analysis (PCA), alongside the power of 3D Convolutional Neural Networks (3D-CNN), the research aims to enhance the precision and efficiency of ore detection in complex geological environments. The proposed method employs a specific 3D-CNN architecture, accepting input as a 7×7×C image patch, where C represents the combined number of selected Aster image bands, Principal Component (PC) bands, and computed BR images. To evaluate the accuracy of the proposed method, five distinct image bands combinations, including the proposed bands combination, were tested and evaluated based on the overall accuracy (OA), average accuracy (AA), and kappa coefficient. The results demonstrated that while the incorporation of BR images alongside Aster bands initially seemed promising, it introduced significant confusion in certain classifications, leading to unexpected mis-classification rates. Surprisingly, utilizing solely Aster bands as input parameters yielded higher accuracy rates (OA= 93.13%, AA = 91.96%, Kappa = 90.91%) compared to scenarios involving the integration with band ratios (OA = 87.02%, AA = 79.15, and Kappa = 82.60%) or the integration of BR images to PC bands (OA= 87.78%, AA = 82.39%, Kappa = 83.81%). However, the amalgamation of Aster bands with selected PC bands showed slight improvements in accuracy (OA= 94.65%, AA = 92.93%, Kappa = 93.45%), although challenges in accurately classifying certain features persisted. Ultimately, the proposed combination of Aster bands, PC bands, and BR images (proposed bands combination) presented the most visually appealing and statistically accurate results (OA = 96.95%, AA= 94.87%, and Kappa = 95.93%), effectively addressing misclassifications observed in the other combinations. These findings underscore the synergistic contributions of each of the Aster bands, PC bands, and BR images, with Aster bands proving pivotal for optimal skarn classification, PC bands enhancing intrusions classification accuracy, and BR images strengthening wall rock classification accuracy. In conclusion, the proposed combination of input image bands emerges as a robust and comprehensive methodology, demonstrating unparalleled accuracy in the remote sensing detection of skarn iron minerals.

Preprint ARTICLE | doi:10.20944/preprints202405.0735.v1

Effects of Adding Sphingomonas Z392 to Drinking Water on Growth Performance, Intestinal Histological Structure and Microbial Community of Broiler Chickens

Mingcheng Wang, Jie Zhong, Yanan Guo, Shuqiang Zhao, Huili Xia, Gailing Wang, Chaoying Liu, Aizhen Guo

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Broiler; Sphingomonas; EPI; Intestinal histology; Intestinal microbiota

Online: 12 May 2024 (07:44:22 CEST)

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Probiotics have emerged as a prominent alternative to antibiotics in antimicrobial-free broiler farming. To assess the effect of the probiotics Sphingomonas sp. Z392 isolated and identified by this this laboratory on broiler growth, we conducted a study to examine the changes in broiler body weight, EPI, intestinal histological structure and gut microbiota. The results showed that supplementation of broiler drinking water with 4 × 105 CFU/mL of Sphingomonas resulted in an increase in the relative abundance of Lactobacillus, Bacteroides, Lachnospiraceae, Aminobacterium, Oribacterium, Christensenellaceae, Faecalibacterium, Barnesiella, Ruminococcus, Parabacteroides, Phascolarctobacterium, Butyricicoccaceae, and Caproiciproducens, which were reported to be positively correlated with the digestion and absorption of broiler chickens. The relative abundance was increased of Odoribacter, Alistipes, Parabacteroides, and Rikenellaceae, which were reported to be negatively correlated with the occurrence of intestinal diseases. The relative abundance was decreased of Campylobacter, Shigella Castellani, Bilophila, Campylobacter, Clostridia, and Anaerotruncus, which were reported positively correlated with the occurrence of intestinal diseases. At the same time, it was increased in the integrity of small intestinal villus morphology, the number of goblet cells in small intestinal epithelial cells, the health of mitochondria in the cytoplasm of jejunal villous epithelial cells, and the number of lysosomes in the cytoplasm of goblet cells in small intestinal epithelium, ileal villous epithelial cells, mitochondria in the cytoplasm of large intestinal villous epithelial cells, the VH/CD of the ileum, digestive, absorption, and defense capabilities. Especially the final weight was increased by 4.33% and the EPI increased by 10.10%. Therefore, the supplementation of broiler drinking water with Sphingomonas generated better economic benefits for the broiler chickens.

Preprint REVIEW | doi:10.20944/preprints202404.1341.v1

From the Gut to the Brain: Is Microbiome a New Paradigm in Parkinson’s Disease Treatment?

Cristiana Vilela, Bruna Araújo, Carla Soares-Guedes, Rita Caridade-Silva, Joana Martins-Macedo, Catarina Teixeira, Eduardo D Gomes, Cristina Prudêncio, Mónica Vieira, Fábio G. Teixeira

Subject: Biology And Life Sciences, Life Sciences Keywords: Parkinson’s Disease; Microbiome; Gut-Brain Axis

Online: 19 April 2024 (15:28:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1721.v1

Deciphering the Anti-Inflammatory Mechanisms of Cirsium japonicum by Combining Network Pharmacology, Molecular Docking and In Vitro Experimental Evaluation

Jiaxue Wang, Hui Tao, Zhenlong Wang, Wei An, Ya Zhao, Bing Han, Jinquan Wang, Xiuzhu Sun, Xiumin Wang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cirsii japonicum; network pharmacology; anti-inflammatory activity; molecular docking; cellular experiment

Online: 23 September 2024 (11:56:21 CEST)

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Cirsium japonicum, a traditional herb, exhibits significant anti-inflammatory activity; however, the main components and potential mechanisms of C. japonicum remain unclear. The aim of this study is to investigate the anti-inflammatory mechanism of Cirsium japonicum through network pharmacology and cellular experiments. The effective components and potential targets for anti-inflammatory activity of C. japonicum were identified using traditional Chinese medicine systematic pharmacology database, TCMSP analysis platform, and GeneCards database. The drug-component-target-disease network diagram was constructed using Cytoscape3.8.0 software, while the protein interaction network diagram was created using STRING database and Cytoscape3.8.0 software. Gene ontology (GO) enrichment and KEGG pathway enrichment analysis were carried out using DAVID database. Molecular docking between key targets and active components was constructed with AutoDock software to determine the best binding target. Results revealed that 14 active components of C. japonicum targeted 171 anti-inflammatory proteins. GO function enrichment analysis yielded 173 items, while KEGG pathway enrichment analysis identified 48 signaling pathways related to inflammation regulation. Molecular docking showed that strong affinity between sitosterol, stigmasterol, and other components with key targets such as peroxisome proliferator-activated receptor α recombinant protein (PPARA) and cyclooxygenase-2 (PTGS2). Vanillin, one active ingredient of C. japonicum, inhibited the release of lipopolysaccharide (LPS)-induced inflammatory factors in RAW264.7 cells. These findings suggest that C. japonicum may exert its anti-inflammatory effects by modulating the phosphatidylinositol 3-kinase (PI3K)/ protein kinase B (Akt) signal pathway (PI3K-Akt) and apoptin signal pathway, highlighting the multi-component, multi-target, and multi-channel molecular mechanism underlying its anti-inflammatory properties. Finally, the anti-inflammatory effect of vanillin, an effective component in C. japonicum, was verified by cell experiments. This study provides a new understanding of the pharmacological mechanisms of C. japonicum in treatment of inflammatory conditions.

Preprint ARTICLE | doi:10.20944/preprints201703.0004.v1

Purification of Polymer-Grade Fumaric Acid from Fermented Spent Sulfite Liquor

Diogo Figueira, João Cavalheiro, Bruno Sommer Ferreira

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: fumaric acid; purification; spent sulfite liquor; biorefineries

Online: 1 March 2017 (09:41:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0473.v1

Glomus highlandensis and G. mongioie, Two New Arbuscular Mycorrhizal Fungi from Saltmarshes, Dunes and Mountains of Europe

Franco Magurno, Sylwia Uszok, Karolina Bierza, Jawdat Bakr, Zoltan Kende, Mariana Bessa de Queiroz, Bruno Tomio Goto

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Glomeromycota; molecular phylogeny; ribosomal variants; taxonomy; two new Glomus species

Online: 7 April 2024 (10:53:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0246.v1

Comprehensive Bio-Screening of Phytochemistry and Biological Capacity of Oregano (Origanum vulgare) and Salvia triloba Extracts against Oral Cariogenic and Food-Origin Pathogenic Bacteria

Maria Antoniadou, Georgios Rozos, Natalia Vaou, Konstantinos Zaralis, Caglar Ersanli, Athanasios Alexopoulos, Aikaterini Dadamogia, Theodoros Varzakas, Athina Tzora, Chrysoula (Chrysa) Voidarou

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: Origanum vulgare; Salvia triloba extracts; phytochemistry and biological capacity; oral cariogenic and food-origin pathogenic bacteria

Online: 6 May 2024 (08:55:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0526.v1

Dynamic Output Feedback of Second-Order Systems: An Observer-Based Controller with LMI Design

Danielle Gontijo, José Mário Araújo, Luciano Frezzato, Fernando de Oliveira Souza

Subject: Engineering, Control And Systems Engineering Keywords: Second-order systems; Output feedback; Functional observer; LMI

Online: 9 May 2024 (09:02:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0570.v2

The Impact of Predation Risks on the Development and Fecundity of the Bactrocera dorsalis Hendel

Xin Liu, Jian Wen, Xingyu Geng, Lu Xiao, Zhe Shan, Yan zhou, Xianli Lu, Yu Fu, ying fu, Fengqin Cao

Subject: Biology And Life Sciences, Insect Science Keywords: Bactrocera dorsalis ; Hendel; non-lethal effects; phenotypic plasticity; predator-prey interaction; pest management

Online: 9 April 2024 (12:31:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0367.v1

Ground Management through Grazing in Rainfed Olive Orchards Provides High Olive Yields and Has Other Potential Benefits for Both the Soil and the Farmer

Paulo Dimande, Margarida Arrobas, Carlos Manuel Correia, Manuel Ângelo Rodrigues

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: soil management; cover cropping; Olea europaea; mixed farming; organic farming; regenerative agriculture

Online: 7 May 2024 (10:29:53 CEST)

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Preprint REVIEW | doi:10.20944/preprints202405.0087.v1

Current Treatment Approaches to Eliminate Borrelia burgdorferi and Potential Strategies to Alleviate Persistent Lyme Disease Symptoms

Kashaf Zafar, Onyedikachi Azuama, Nikhat Parveen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Lyme disease; Borrelia burgdorferi; Treatment; antimicrobials; Post-treatment Lyme disease syndrome; PTLDS

Online: 2 May 2024 (12:03:42 CEST)

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Preprint ESSAY | doi:10.20944/preprints202406.0903.v1

Handling Bathymetry in Geoflood

Mark Mulwana

Subject: Computer Science And Mathematics, Computational Mathematics Keywords: 1. Bathymetry
2. Shallow Water Equations
3. Bilinear Integrals
4. Piece Wise bilinear Surface

Online: 13 June 2024 (09:05:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0364.v1

An Explainable AI-based Machine Learning Approach for Predicting Diabetes in the Early Stage Using the Influential Features

Utsha Das, Boshir Ahmed

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: communicable disease; diabetes; feature selection; imbalanced dataset; insulin; kernel function; outlier
samples

Online: 12 June 2024 (14:16:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1122.v1

How Satellite Imaging and Deep Learning Are Influenced by Tensor Decompositions: A Review

Ismail A. Mageed

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Tensor Decompositions (TDs); Satellite Imaging (SI); Deep Learning (DL); Tensor Train Networks (TTNs); Tucker decomposition (TUD); canonical polyadic decomposition (CPD); polyadic decomposition (PD); Roll, Pitch, and Yaw (RPY)

Online: 20 February 2024 (09:08:09 CET)

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Working Paper ARTICLE

Photonic RF and Microwave Arbitrary Waveform Generator Based on a Soliton Crystal 49GHz Kerr Micro-comb

David Moss, Mengxi Tan, Xingyuan Xu, Andreas Boes, Bill Corcoran, Jiayang Wu, Thach Nguyen, Sai Chu, Brent Little, Roberto Morandotti, Arnan Mitchell

Subject: Engineering, Electrical And Electronic Engineering Keywords: nonlinear optics; microwave; waveform generator; microcombs

Online: 15 May 2020 (09:57:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0829.v1

Toward the Development of Garlic Varieties: The First Attempts

Ricardo Julián Licea-Moreno, Ángeles Rodríguez Haro, Juan Antonio Marín Martínez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Allium sativum (L.); clonal selection; genotype-environment interaction; landrace; plant breeding; selection pressure

Online: 12 June 2024 (17:43:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0303.v1

Characterisation of the Mitochondrial Genome of Capulus danieli: Insights into Evolutionary History and Ecological Adaptations

Yaoyu Xie, Yida Han, Junxia Mao, Xubo Wang, Zhenlin Hao, Ying Tian

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Capulus danieli; mitochondrial genome; protein-coding genes; divergence time estimation

Online: 6 August 2024 (03:19:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1998.v1

Detection of Apple Proliferation Disease by Hyperspectral Sensors and Machine Learning Based Image Analysis

Uwe Knauer, Sebastian Warnemünde, Patrick Menz, Bonito Steffen Thielert, Lauritz Klein, Katharina Holstein, Miriam Runne, Wolfgang Jarausch

Subject: Biology And Life Sciences, Plant Sciences Keywords: hyperspectral; random forest; machine learning; apple; ‘ Candidatus Phytoplasma mali’ ; disease monitoring

Online: 25 September 2024 (12:16:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0367.v1

Graphene Oxide Thin Films for Integrated Electronics and Photonics

David Moss

Subject: Physical Sciences, Applied Physics Keywords: Graphene oxide; 2D materials; integrated optics; nonlinear optics; photonic integrated circuits

Online: 20 December 2022 (10:28:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0629.v1

Chemometric Analysis of GC-MS Chemical Profiles and Biological Activities of Three Citrus Essential Oils in Indonesia

Einstivina Nuryandani, Erliana Novitasari, Muhammad Fatih Abdurrahman, Nurul Habibi, Ardiba Rakhmi Sefrienda, Dedy Kurnianto, Jasmadi Jasmadi, Yusuf Andriana

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Citrus species, particle component analysis, essential oils, antioxidant activity, cytotoxicity effects, chemometric analysis

Online: 10 May 2024 (09:11:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1339.v1

Pioneering the Future of Industrial Development: Harnessing Membrane Separation Breakthroughs to Propel CO₂ Extraction from Natural Gas into a Sustainable Era

NNADIKWE JOHNSON, EWELIKE ASTERIUS DOZIE, Abdulazeez Adeyemi, Momoh, Onuabuchi Azunna

Subject: Engineering, Energy And Fuel Technology Keywords: Process Design; Natural Gas Separation; ; Gas Processing; ; Removal of CO₂; Membrane Process

Online: 19 December 2023 (04:52:56 CET)

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Natural Gas (NG) processing is a prominent industrial separation process. Among the available techniques, the innovative membrane process shows potential for efficient removal of impurities, including carbon dioxide (CO₂). This study focuses on utilizing breakthroughs in membrane separation to drive sustainable CO₂ extraction from natural gas. Through comprehensive research and analysis, we explore the effectiveness and feasibility of membrane-based systems in removing CO₂ impurities from NG, thus promoting greener and more sustainable industrial practices. Our findings underscore the transformative nature of membrane separation technology, presenting new possibilities for a more environmentally-friendly and sustainable approach to CO₂ extraction from natural gas.Natural Gas (NG) processing utilizes various techniques for impurity removal, with the membrane process emerging as a promising option for efficient carbon dioxide (CO₂) extraction. This research proposes the integration of a simple mathematical model into ASPEN HYSYS to design a membrane system for CO₂/CH₄ separation. The study also investigates parameter sensitivities by altering operating conditions, such as feed composition and pressure, as well as membrane properties, including selectivity. By analyzing these variables, we aim to optimize the performance and efficiency of the membrane system, facilitating the sustainable extraction of CO₂from NG. The findings contribute to advancing the design and operation of membrane-based processes for CO₂ separation, paving the way for greener and more sustainable industrial practices. In addition, this study explores various configurations for optimizing the design of the membrane system, including single-stage with and without recycling as well as double-stage configurations. The investigation demonstrates that methane recovery can be enhanced through the recycling of the permeate stream and by implementing a double stage membrane system. These findings highlight the potential for improving the efficiency and performance of the membrane system, enabling higher methane recovery rates. By considering different configurations, this research contributes to the development of more effective and sustainable CO₂ extraction processes from natural gas.

Preprint ARTICLE | doi:10.20944/preprints202402.1231.v1

The L1014F Knockdown Resistance Mutation Is Not a Strong Correlate of Phenotypic Resistance to Pyrethroids in Florida Populations of Culex quinquefasciatus

Alden S Estep, Neil D Sanscrainte, Jason Stuck, Isik Unlu, Agne Prasauskas, Stephanie J Mundis, Nicholas Cotter, Ana L Romero-Weaver, Troy J Fedirko, Natalie L Kendziorski, Kyle J Kosinski, Daviela Ramirez, Eva A Buckner

Subject: Biology And Life Sciences, Insect Science Keywords: Culex quinquefasciatus; insecticide resistance; knockdown resistance (kdr); CDC bottle bioassay; Florida

Online: 23 February 2024 (06:26:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.1685.v1

Nutritive Value and Degradation Kinetic Parameters of Three Plants for Feeding Bradypus variegatus Schinz

Igor Luiz Carvalho Máximo, Júlio Cézar dos Santos Nascimento, Gilcifran Prestes de Andrade, José Lypson Pinto Simões Izidro, Priscilla Virgínio de Albuquerque, Daniel Bezerra do Nascimento, Janerson José Coêlho, Marleyne José Afonso Accioly Lins Amorim, Alexandre Carneiro Leão de Mello, Romero Marcos Pedrosa Brandão Costa, Carlos Bôa-Viagem Rabello, Maria do Carmo Mohaupt Marques Ludke, Apolônio Gomes Ribeiro, Ricardo Alexandre Silva Pessoa

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: bradypodidae; chemical composition; Inga sp.; in vitro kinetics; Pterodon sp.

Online: 22 July 2024 (07:16:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0369.v1

Vitis rotundifolia Genes Introgressed with RUN1 and RPV1: Poor Recombination and Impact on v. vinifera Berry Transcriptome

Mengyao SHI, Stefania Savoi, Gautier Sarah, Alexandre Soriano, Audrey Weber, Laurent Torregrosa, Charles Romieu

Subject: Biology And Life Sciences, Plant Sciences Keywords: Hybrids; Fruit development; V. rotundifolia; V. vinifera; Transcriptome; Resistance genes

Online: 6 June 2024 (11:42:44 CEST)

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Thanks to several Vitis vinifera backcrosses with an initial V. vinifera L. × V. rotundifolia (previously Muscadinia rotundifolia) interspecific cross, the MrRUN1/MrRPV1 locus (resistance to downy and powdery mildews) was introgressed in genotypes phenotypically close to V. vinifera varieties. To check the consequences of introgressing parts of the V. rotundifolia genome on gene expression during fruit development, we conducted a comparative RNA-seq study on single berries from different V. vinifera cultivars and V. vinifera × V. rotundifolia hybrids, including ‘G5’ and two derivative microvine lines, ‘MV102’ (resistant) and ‘MV32’ (susceptible) segregating for the MrRUN1/RPV1 locus. RNA-Seq profiles were analyzed on a comprehensive set of single berries from the end of the herbaceous plateau to the ripe stage. Pair-end reads were aligned both on V. vinifera PN40024.v4 reference genome, V. rotundifolia cv ‘Trayshed’ and cv ‘Carlos’, and to the few resistance genes from the original V. rotundifolia cv ‘52’ parent available at NCBI. Weighted Gene Co-expression Network Analysis (WGCNA) led to classifying the differentially expressed genes into 15 modules either preferentially correlated with resistance or berry phenology and composition. Resistance positively correlated transcripts predominantly mapped on the 4-5 Mb distal region of V. rotundifolia chromosome 12 beginning with the MrRUN1/MrRPV1 locus, while the negatively correlated ones mapped on the orthologous V. vinifera region, showing this large extremity of LG12 remained recalcitrant to internal recombination during the successive backcrosses. Some constitutively expressed V. rotundifolia genes were also observed at lower density outside this region. Genes overexpressed in developing berries from resistant accessions, either introgressed from V. rotundifolia, or triggered by these in the vinifera genome, spanned various functional groups, encompassing calcium signal transduction, hormone signaling, transcription factors, plant–pathogen-associated interactions, disease resistance proteins, ROS and phenylpropanoid biosynthesis. This transcriptomic insight provides a foundation for understanding the disease resistance inherent in these hybrid cultivars and suggests a constitutive expression of NIR NBS LRR triggering calcium signaling. Moreover, these results illustrate the magnitude of transcriptomic changes caused by the introgressed V. rotundifolia background in backcrossed hybrids, on a large number of functions largely exceeding the ones constitutively expressed in single resistant gene transformants.

Preprint ARTICLE | doi:10.20944/preprints202404.1987.v1

Low Plasma Lipids are Associated with Relapsing and Lethal Visceral Leishmaniasis in HIV-Infected Patients

Renata V. S. Silva, Silvia R.B. Uliana, Jenicer K.U.Y. Yasunaka, Cláudio S. Veloso, Emille A Sousa, Maria Michelle L. Ferreira, Vivianne S. Carvalho, Gabriel R. Ferreira, Dorcas L. Costa, Carlos H. N. Costa

Subject: Medicine And Pharmacology, Internal Medicine Keywords: Visceral leishmaniasis; Leishmania infantum; HIV/AIDS; Lipid profile; Cachexia

Online: 30 April 2024 (11:42:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0075.v1

Obesogenic Gut Microbiota as a Crucial Factor Defining the Depletion of Enzyme Representation for Vitamin B12 Synthesis in the Mouse Intestine

Anastasia A. Zabolotneva, Irina M. Kolesnikova, Ilya Yu. Vasiliev, Tatiana V. Grigoryeva, Sergei A. Roumiantsev, Aleksandr V. Shestopalov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: gut microbiota; obesity; cobalamin; vitamin b12; db/db mice

Online: 2 April 2024 (04:10:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202012.0009.v1

Review of Fresnel Incoherent Correlation Holography with Linear and Non-Linear Correlations

Vijayakumar Anand, Tomas Katkus, Soon Hock Ng, Saulius Juodkazis

Subject: Physical Sciences, Acoustics Keywords: Holography; diffractive optics; incoherent optics; Fresnel incoherent correlation holography; Imaging; speckle

Online: 1 December 2020 (09:55:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202409.1066.v1

Nitrogen Assimilation, Biomass and Yield in Response to Application of Algal Extracts, Rhizobium sp. and Trichoderma asperellum, as Biofertilizers in Hybrid Maize

Sandra Pérez-Álvarez, Erick H. Ochoa-Chaparro, Julio César Anchondo-Páez, César M. Escobedo-Bonilla, Joel Rascón-Solano, Marco A. Magallanes-Tapia, Luisa Patricia Uranga-Valencia, Reinier Hernández-Campos, Esteban Sánchez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: algal extracts; biofertilizers; nitrogen assimilation; Rhizobium sp.; Trichoderma asperellum; Zea mays L.

Online: 13 September 2024 (08:46:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1652.v1

Bacterial Community in Fresh Fruits and Vegetables Sold in Streets and Open-Air Markets of Dakar, Senegal

Sarbanding Sane, Sophie D. Tene, Abou Abadallah Malick Diouara, Seynabou Coundoul, Malick Mbengue, Yakhya Dieye

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Food safety; Fresh fruits and vegetables; E. coli; Vibrio spp; Salmonella spp; metagenomic

Online: 27 November 2023 (11:11:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1588.v1

Microbiome Dynamics of Salmo salar Skin and Gill

Marcos Godoy, Yoandy Coca, Rudy Suárez, Marco Montes de Oca, Jacob W. Bledsoe, Ian Burbulis, Diego Caro, Juan Pablo Pontigo, Vinicius Maracaja-Coutinho, Raúl Arias-Carrasco, Leonardo Rodríguez-Córdova, César Sáez-Navarrete

Subject: Biology And Life Sciences, Aquatic Science Keywords: Piscirickettsia salmonis; Salmo salar; skin; ulcer; microbiome

Online: 24 November 2023 (09:25:08 CET)

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Working Paper ARTICLE

Large Negative Self-Defocusing Kerr Nonlinearity in 2D PdSe₂ Dichalcogenide Films Measured by Z-Scan

Linnan Jia, Jiayang Wu, Tieshan Yang, Baohua Jia, David J. Moss

Subject: Physical Sciences, Optics And Photonics Keywords: nonlinear optics

Online: 2 May 2020 (12:33:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0563.v1

Tetracycline Resistance Genes in Treponema spp.- an Analysis of 4355 Spirochaetales Genomes

Sheeba Manoharan-Basil, Zina Gestels, Said Abdellati, Irith De Baetselier, Thibaut Vanbaelen, Chris Kenyon

Subject: Biology And Life Sciences, Life Sciences Keywords: Syphilis; Tetracycline resistance; Treponema pallidum

Online: 11 March 2024 (08:59:05 CET)

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Preprint REVIEW | doi:10.20944/preprints202408.1936.v2

Artificial Intelligence in Cryo-EM Protein Particle Picking: Recent Advances and Remaining Challenges

Ashwin Dhakal, Rajan Gyawali, Liguo Wang, Jianlin Cheng

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cryo-Electron Microscopy; Protein Particle Picking; Artificial Intelligence; Machine Learning; Structural Biology

Online: 2 September 2024 (05:09:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1963.v1

Protonation Is Part of Dielectric Relaxation upon Reduction of Bacteriopheophytin in Reaction Center of Photosynthetic Bacterium Rhodobacter sphaeroides

Gábor Sipka, Péter Maróti

Subject: Biology And Life Sciences, Biophysics Keywords: Rhodobacter sphaeroides; reaction center; bacteriophyeophytin; quinone substitution; acidic cluster; thermodynamics; delayed fluorescence

Online: 25 September 2024 (08:59:03 CEST)

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The pH dependence of the free energy level of the flash induced primary charge pair P+IA– was determined by combination of the results from indirect charge recombination of P+QA– and from delayed fluorescence of the excited dimer (P*) in reaction center of photosynthetic bacterium Rhodobacter sphaeroides where the native ubiquinone at the primary quinone binding site QA was replaced by low potential anthraquinone derivatives. The following observations were made: 1) The free energy state of P+IA– was pH independent below pH 10 (–370 ± 10 meV relative to that of the excited dimer P*) and showed remarkable decrease (about 20 meV/pH unit) above pH 10. A not insignificant part of the dielectric relaxation of the P+IA– charge pair (about 120 meV) should come from protonation related changes. 2) The single exponential decay character of the kinetics proves that the protonated/unprotonated P+IA– and P+QA– states are in equilibria and the rate constants of protonation konH +koffH is much larger than that of the charge back reaction kback ~103 s-1. 3) Highly similar pH profiles were measured for the free energy states of P+QA– and P+IA–, indicating that the same acidic cluster around QB should response to both anionic species. This was supported by model calculations based on anticooperative proton distribution in the cluster with key residues of GluL212, AspL213, AspM17 and GluH173 and on effect of polarization of the aqueous phase on the electrostatic interaction. The larger distance of IA– from the cluster (25.2 Å) than that of QA‒ (14.5 Å) is compensated by smaller effective dielectric constant (6.5 ± 0.5 and 10.0 ± 0.5, respectively). 4) The P* → P+QA− and IA‒QA → IAQA− electron transfers are enthalpy driven reactions with exemption of very large (>60%) or negligible entropic contributions in cases of substitution by 2,3-dimethyl-AQ or 1-chloro-AQ, respectively. The possible structural consequences are discussed.

Preprint REVIEW | doi:10.20944/preprints202401.1280.v1

Burkholderia pseudomallei Complex Subunit and Glycoconjugate Vaccines and Their Potential to Elicit Cross-Protection to Burkholderia cepacia complex.

Alexander J Badten, Alfredo G Torres

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Burkholderia pseudomallei; Burkholderia mallei; Burkholderia cepacia complex; melioidosis; glanders; cystic fibrosis; vaccines; subunit; glycoconjugate

Online: 17 January 2024 (08:35:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0775.v1

Classifying Objects in 3D Point Clouds Using Recurrent Neural Network: A GRU LSTM Hybrid Approach

Ramin Mousa, Mitra Khezli, Saba Hesaraki

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Point Cloud Classification; Virtual Reality; Hybrid Model; GRULSTM;
GRU; LSTM

Online: 13 March 2024 (10:00:29 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1060.v1

Dermatophyte Infections Worldwide: Increase in Incidence and Associated Antifungal Resistance

Caroline Kruithoff, Ahmed Gamal, Thomas S. McCormick, Mahmoud A. Ghannoum

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Incidence; Terbinafine; Dermatophytes; Trichophyton; T. rubrum; T. mentagrophytes; T. indotineae; Superficial Fungal Infection

Online: 16 November 2023 (11:22:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1307.v1

Cold-Azurin a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium Pseudomonas sp. TAE6080

Caterina D’Angelo, Marika Trecca, Andrea Carpentieri, Marco Artini, Laura Selan, Maria Luisa Tutino, Rosanna Papa, And Ermenegilda Parrilli

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Staphylococcus epidermidis; antibiofilm; Azurin; cold-adapted bacteria

Online: 18 January 2024 (03:13:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0882.v1

Sexual Rehabilitation Strategies and Outcomes among Cervical Cancer Survivors A

Samson Peter Mvandal, Mashaka Sehemu

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: cervical cancer; cervical cancer survivors; rehabilitation; sexual dysfunction; sexual rehabilitation
trategies

Online: 13 May 2024 (16:23:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1187.v1

Toward Enhanced Plant Immunity: Unveiling the Role of Brassinosteroids in Abiotic and Biotic Stress Response

Barbara Sawicka*, Piotr Barbaś, Bernadetta Bienia, Dominika Skiba, Piotr Pszczółkowski, Barbara Krochmal-Marczak

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: plant hormones; gene expression; BR functions; antimicrobial actions; immunity mechanisms; plant stress

Online: 21 February 2024 (04:00:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.2035.v1

Controlled Quantum Communication Using Quantum Random Walk

Subham Das, A.V.N.S. Meghanath, Rajiuddin Sk, Prasanta K. Panigrahi

Subject: Physical Sciences, Quantum Science And Technology Keywords: Quantum Random Walk, Controlled Quantum
communication, Weak Measurement

Online: 30 May 2024 (13:17:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0897.v1

Sterility Mosaic Disease of Pigeonpea (Cajanus cajan (L.) Huth): Current Status, Disease Management Strategies, and Future Prospects

BR Sayiprathap, Anil Kumar Patibanda, Muttappagol Mantesh, Shridhar Shivakumar Hiremath, N SAGAR, CN Lakshminarayana Reddy, CR Jahir Basha, SE Diwakar Reddy, Mediga Kasi Rao, Ramakrishnan Madhavan Nair, Hari Kishan Sudini

Subject: Biology And Life Sciences, Plant Sciences Keywords: Cajanus cajan; SMD; PPSMV; eriophyid mite; screening; disease management

Online: 13 June 2024 (07:10:51 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202405.0234.v1

Microstructure and Elemental Composition of Coffea arabica L. Roasted Medium of Specialty and with “Pluma” Denomination of Origin

Jesica Jiménez, Luis Robledo, Ema Antonio, Alma Pérez, Marco Sánchez, Iván García

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Coffea arabica L; microstructure; elemental composition; roasted medium; “ Pluma”

Online: 6 May 2024 (08:11:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1537.v1

Converting Candlenut Shell Waste into Graphene for Electrode Applications

Rikson Siburian, Kerista Tarigan, Yosia Gopas Oetama Manik, Fajar Hutagalung, Yatimah Alias, Yong Ching Chan, Boon Peng Chang, Jonathan Siow, Amanda Jiamin Ong, Jingfeng Huang, Suriati Paiman, Boon Tong Goh, Lisnawaty Simatupang, Ronn Goei, Alfred Iing Yoong Tok, Yahya Mohd Fakharul Zaman Raja, Fathan Bahfie

Subject: Engineering, Other Keywords: graphene; candlenut (Aleurites moluccanus) shell; Ce/graphene; carbon large scale production; energy storage electrodes

Online: 24 June 2024 (04:28:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1855.v1

Antimicrobial Susceptibility of Uropathogenic Escherichia coli with Analysis of Impact of Socio-Economic Factors in Low, Medium and High-Income Countries

Meher Rizvi, Maria Khan, Amina AL Jardani, Ujjala Ghoshal, Zaaima AL Jabri, Mohammad Ahanjan, Azza AL Mamari, Nawal AL Shizawi, Hiba Sami, Abdullah Balkhair, Adila Shaukat, Alwarith Nasser Salem Alkharusi, Abdulrahman Almahrouqi, Afreenish Hassan, Ajay Kumar, Amal Malehi, Amina Gul, Ashima Singh, Asma Said Hamed AL Shidhani, Areena Hoda Siddiqui, Aruna Poojary, Azza AL Rashdi, Bradley Langford, Dmytro Stepanskyi, Abbas Dolatabadi, Aisha Al Mufraji AL Mufraji, Elham Said Ahmed AL Risi, Garima Kapoor, Isabella Princess, Hatim Ali Eltahir, Hilal Al Sidairi, Kaitlyn Dougherty, Katia Iskandar, Ken Masters, Ibrahim Khalaf Hamdan AL Busaidi, Laila AL Yazidi, Mahfuza Nasrin, Mahmood Salim AL Subhi, Mallika Sengupta Mallika Sengupta, Manisha Khandait, Nada Khalfan AL Tamtami, Nada Khafan Alsiyabi, Nawal AL Kindi, Nazla Musthafa Luthfee, Neha Shreshtha, Nihal Mohamed Amur AL Riyami, Noora Hilal Ali AL Busaidi, Nupur Goel, Oksana Ishchenko, Omnia Mohamed Elnabawy Ahmed Taher, Pragnya Paramita Jena, Rajni Ekadashi, Razan Zadjali, Reba Kanungo, Sara Abolghasemi, Salima Al-maqbali, Sanjay Jaju, Sayantan Banerjee, Shadma Yaqoob, Shahnaz Sali, Shafqat Husnain Khan, Shalini Malhotra, Stephen Hughes, Sundas Shaukat, Syed Mohammed Atif, Vrushali Vishwas Patwardhan, Victorien Dougnon, Wahid Khan, Walid Wali, Zakariya AL Muharrmi, Zeeshana Basit, Jasashree Choudhury, Rachita Pravalina, Sanjeev Singh, Sarman Singh, Neelam Taneja, Raman Sardana, Pawan Kapoor, Rajeev Soman, Rashid Al Abri

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: community acquired UTI; Escherichia coli; antimicrobial susceptibility; Indian subcontinent; MENA; GDP; temperature

Online: 24 September 2024 (12:32:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0534.v1

Using Acanthamoeba spp. as a Cell Model to Evaluate Leishmania Infections

Helena Santos, Gabriella Linhares, Rhagner Bonono do Reis, Igor Cardoso, Claudia Masini d'Avila, Vitor Ennes Vidal

Subject: Biology And Life Sciences, Parasitology Keywords: Acanthamoeba castellanii, Acanthamoeba polyphaga, Leishmania, interaction host-parasite

Online: 5 July 2024 (12:20:28 CEST)

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Leishmaniasis represents a severe global health problem. In the last decades, there have been significant challenges in controlling this disease due to the unavailability of licensed vaccines, the high toxicity of the available drugs, and an unrestrained surge of drug-resistant parasites, and human immunodeficiency virus (HIV)–Leishmania co-infections. Leishmania spp. preferentially invade macrophage lineage cells of vertebrates for replication after subverting cellular functions of humans and other mammals. These early events in host–parasite interactions are likely to influence the future course of the disease. Thus, there is a continuing need to discover a simple cellular model that reproduces the in vivo pathogenesis. Acanthamoeba spp. are non-mammalian phagocytic amoeba with remarkable similarity to the cellular and functional aspects of macrophages. We aimed to assess whether the similarity reported between macrophages and Acanthamoeba spp. is sufficient to reproduce the infectivity of Leishmania spp. Herein, we analyzed co-cultures of Acanthamoeba castellanii or Acanthamoeba polyphaga with Leishmania infantum, Leishmania amazonensis, Leishmania major, and Leishmania braziliensis. Light and fluorescence microscopy revealed that the flagellated promastigotes attach to the A. castellanii and/or A. polyphaga in a bipolar and or random manner, which initiates their uptake via pseudopods. Once inside the cells, the promastigotes undergo significant changes, which result in the obligatory amastigote-like intracellular form. There was a productive infection with a continuous increase in intracellular parasites. However, we frequently observed intracellular amastigotes in vacuoles, phagolysosomes, and the cytosol of Acanthamoeba spp. Our findings corroborate that Leishmania spp. infects Acanthamoeba spp. and replicates in them but does not cause their rapid degeneration or lysis. Overall, the evidence presented here confirms that Acanthamoeba spp. have all prerequisites and can help elucidate how Leishmania spp. infect mammalian cells. Future work exposing the mechanisms of these interactions should yield novel insights into how these pathogens exploit amoebae.

Preprint ARTICLE | doi:10.20944/preprints202404.0685.v1

Traffic Safety Analysis at Signalized Intersections during Work Zones Intervals by Using the LiDAR Sensor Technology

Alireza Ansariyar

Subject: Engineering, Civil Engineering Keywords: LiDAR Sensor, Signalized Intersections, Work Zone Safety, Traffic Analysis, Work Zone Management

Online: 10 April 2024 (09:23:10 CEST)

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The importance of conflict analysis and safety considerations during work zone intervals at signalized intersections cannot be overstated, as these periods pose unique challenges and safety risks for both motorists and pedestrians. Work zones introduce temporary changes to traffic patterns, lane configurations, and signal timings, which can disrupt the flow of traffic and increase the likelihood of conflicts between vehicles and pedestrians. By conducting comprehensive conflict analysis, it is possible to identify potential conflict points, such as merging areas, pedestrian crossings, and lane transitions, and implement targeted safety measures to mitigate risks. Additionally, considering safety considerations during work zone intervals is paramount to safeguarding the well-being of road users and minimizing the occurrence of conflicts or collisions. Measures such as enhanced signage, temporary traffic control devices, and increased enforcement efforts can help promote compliance with traffic regulations and ensure the safe passage of vehicles and pedestrians through work zones. This study investigates the dynamics of traffic behavior and safety implications within work zones at signalized intersections, aiming to address key challenges and enhance intersection management strategies. Through comprehensive analysis, varying frequencies of Vehicle-to-Vehicle (V2V) conflicts were observed across different movement directions, with significant occurrences identified in specific phases of the traffic signal cycle. Specifically, 69 V2V conflicts in phase #1, 181 in phase #2, 31 in phase #3, 207 in phase #4, 1 in phase #5, 274 in phase #6, 93 in phase #7, and 186 in phase #8 were recorded. Furthermore, the examination of Vehicle-to-Pedestrian (V2P) conflicts underscored the importance of prioritizing pedestrian safety, with notable occurrences observed in phases associated with pedestrian crossing movements. Meanwhile, despite the closure of left-turn movements, instances of red light running persisted, with 69 red light running events in phase #1, 181 in phase #2, 31 in phase #3, 207 in phase #4, 1 in phase #5, 274 in phase #6, 93 in phase #7, and 186 in phase #8.To mitigate these safety concerns during work zone intervals, proactive measures are essential. Strategies include improving communication through clear signage and advanced warning systems, enhancing enforcement efforts with red light cameras and increased police presence, and investing in infrastructure improvements such as temporary pedestrian facilities. Integration of real-time data from sensors, particularly LiDAR technology, offers significant advantages in detecting conflicts and red light runners during work zone intervals. LiDAR's precise, real-time data on vehicle and pedestrian movements, combined with its 360-degree coverage and high spatial resolution, facilitates accurate detection and tracking of potential conflict scenarios, contributing to enhanced intersection safety and traffic management efficiency. This study endeavored to conduct a traffic safety analysis during work zone intervals at a signalized intersection in Baltimore City, known for its high rate of traffic crashes, utilizing LiDAR sensor technology.

Preprint ARTICLE | doi:10.20944/preprints201808.0428.v1

Dual Functional Ultrafiltration Membranes with Enzymatic Digestion and Thermo-Responsivity for Protein Self-Cleaning

Anbharasi Vanangamudi, Ludovic F. Dumée, Mikel Duke, Xing Yang

Subject: Chemistry And Materials Science, Nanotechnology Keywords: thermo-responsive; ultrafiltration; enzymes; self-cleaning; nanofibers

Online: 24 August 2018 (07:52:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1143.v1

Finite and Symmetric Euler Sums and Multiple T-Values

Jianqiang Zhao

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: (finite) Euler sums; symmetric Euler sums; (finite) multiple T-values; symmetric multiple T-values; alternating multiple T-values

Online: 20 February 2024 (14:34:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.1333.v1

Effects of a Bank Plant on the Control of Rice Planthoppers: A Field Study

Xin Guo, Qiao-yu Zhou, Meng-meng Ru, Bei Cao, Xin Huang, Xu-song Zheng, Ping-yang Zhu

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Leersia sayanuka; bank plant; sesame; rice; ecological engineering for pest control

Online: 21 May 2024 (04:48:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2001.v1

Development of an Eco-Friendly Nanogel Incorporating Pectis brevipedunculata Essential Oil as Larvicidal Agent Against Aedes aegypti

Estela Mesquita Marques, Raiene Lisboa Rocha, Clenilma Marques Brandão, Júlia Karla de Albuquerque Melo Xavier, Marcos Bispo Pinheiro Camara, Caritas de Jesus Silva Mendonça, Roberto Batista de Lima, Melissa Pires Souza, Emmanoel Vilaça Costa, Renato Sonchini Gonçalves

Subject: Chemistry And Materials Science, Nanotechnology Keywords: thermoresponsive nanogel; Pectis brevipendunculata essencial oil; Aedes aegypti

Online: 25 September 2024 (12:10:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2401.v1

Staphylococcus aureus Stress Response to Bicarbonate Depletion

Elisa Liberini, Sook-Ha Fan, Arnold S. Bayer, Christian Beck, Jacob Biboy, Patrice François, Joe Gray, Katharina Hipp, Iris Koch, Andreas Peschel, Brigitte Sailer, Daniela Vollmer, Waldemar Vollmer, Friedrich Götz

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Staphylococcus aureus; bicarbonate transporter; MpsABC; cell wall; peptidoglycan; transcriptome

Online: 30 July 2024 (11:03:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0764.v1

Comparison of Trapping Efficacy of Locally Modified Gravid Aedes Trap and Autocidal Gravid Ovitrap for the Monitoring and Surveillance of Aedes aegypti Mosquitoes in Tanzania

Jane Johnson Machange, Masudi Suleiman Maasayi, John Mungi, Jason Moore, Joseph Barnabas Muganga, Olukayode G. Odufuwa, Sarah J. Moore, Frank Chelestino Tenywa

Subject: Biology And Life Sciences, Insect Science Keywords: Aedes aegypti; mosquito traps; vector control; Bio-Gents Sentinel trap; Gravid Aedes Trap; Autocidal Gravid Ovitrap; Tanzania

Online: 11 April 2024 (14:11:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1156.v1

A High-Homology Region in Bacterial β-Barrel Pore-Forming Toxins

Alexey S. Nagel, Olesya Vetrova, Natalia V. Rudenko, Anna P. Karatovskaya, Anna Zamyatina, Zhanna I. Andreeva-Kovalevskaya, Vadim Salyamov, Nadezhda Egorova, Alexander V. Siunov, Tatiana D. Ivanova, Khanafi M. Boziev, Fedor A. Brovko, Alexander S. Solonin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Staphylococcus aureus α -toxin; Bacillus cereus hemolysin II; cytotoxin K2; suppression of hemolytic activity; monoclonal antibodies; enzyme immunoassay

Online: 20 March 2024 (12:23:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0470.v1

Identification and Characterization of AREB/ABF Gene Family in Three Orchid Species and Functional Analysis of DcaABI5 in Arabidopsis

Xi Xie, Miaoyan Lin, Gengsheng Xiao, Qin Wang, Zhiyong Li

Subject: Biology And Life Sciences, Plant Sciences Keywords: Orchid; gene family; AREB/ABF genes; cis-regulatory elements (CREs) analysis; gene expression; abscisic acid (ABA); ABA INSENSITIVE 5 (ABI5)

Online: 8 February 2024 (07:32:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1615.v2

Adherence to Antiretroviral Therapy (ART) Among HIV-1 Patients From Sub-Saharan Africa: A Systematic Review

Pedro Almeida, Carlos Rafael, Victor Pimentel, Ana Abecasis, Cruz Sebastião, Joana Morais

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: HIV-1; Antiretroviral therapy; Treatment adherence; sub-Saharan Africa.

Online: 29 April 2024 (03:59:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1775.v1

Exploratory Study of Guanidine Derivatives as Novel Anti Trypanosoma cruzi Scaffolds

Eduardo Henrique Zampieri, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Predrag Kalaba, Alexandre Prado-Roller, Natalie Gajic, Martin Zehl, Ana Fabisikova, Luana Ribeiro dos Anjos, Luana Passianoto Gushiken, Gert Lubec, Eduardo Rene Perez Gonzalez

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Chagas disease; Trypanosoma cruzi; guanidines; in vitro trypanocidal activity; cytotoxic assays

Online: 23 July 2024 (10:00:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0679.v1

Detection of Different Lemon Pre-Harvest Additive Treatments by Means an Electronic Nose Prototype

Antonio Ruiz-Canales, Gemma Martínez-Muñoz, Agustín Conesa-Celdrán, ISABEL CÁNOVAS FLORES, Daniel Martin Vertedor, Martin J Oates

Subject: Biology And Life Sciences, Food Science And Technology Keywords: resistive sensors; monitoring; postharvest; Citrus limon

Online: 11 June 2024 (10:57:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0922.v1

Mortality of Boreal Trees

Petri P. Kärenlampi

Subject: Biology And Life Sciences, Forestry Keywords: Picea abies; Pinus sylvestris; Betula pendula; Betula pubescens; logistic regression

Online: 15 April 2024 (10:19:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0478.v1

Novel Multifunctional Luminescent Electrospun Fluorescent Nanofiber Chemosensors-Filter and Their Versatile Sensing of pH, Temperature, and Metal Ions

Bo-Yu Chen, Yen-Chen Lung, Chi-Ching Kuo, Fang-Cheng Liang, Tien-Liang Tsai, Dai-Hua Jiang, Toshifumi Satoh, Ru-Jong Jeng

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: electrospun nanofibers; pH; mercury ion; chemosensor; thermo-response

Online: 22 October 2018 (07:42:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1100.v1

Identification of the Carboxylesterase, Nl-EST1 Gene Expression Regulation Possibly Involved in Carbamate Insecticide Resistance in Brown Planthopper Nilaparvata lugens

Murtaza Khan, Changhee Han, Nakjung Choi, Juil Kim

Subject: Biology And Life Sciences, Insect Science Keywords: Brown planthopper; carbamate resistance; RNA-seq analysis; Nl-EST1; metabolic resistance

Online: 16 August 2024 (03:33:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1026.v1

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

Omar E. Ahuatzin-Flores, Eduardo Torres, Edith Chávez-Bravo

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Acinetobacter baumannii; multi-resistance; natural habitats; reservoirs

Online: 19 February 2024 (08:19:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1413.v1

Characterization of O26 Enteropathogenic E. coli and Its Impli-Cation for Vaccine Development

Thiago Jordão da Silva Lemos, Herbert Guimarães de Sousa Silva, José Osvaldo Previato, Lucia Mendonça-Previato, Elisangela Oliveira de Freita, Angela Silva Barbosa, Marcia Regina Franzolin, Luis Fernando dos Santos, Bruna de Sousa Melo, Geovana Ferreira dos Anjos, Renata Hiromi Nakagima Gonçalves, Marta de Oliveira Domingos

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: E. coli 1; polysaccharide O26 2; vaccine 3; enteropathogenic E. coli; EPEC; shiga toxin-producing E. coli; STEC; enterohemorrhagic E. coli; EHEC

Online: 19 December 2023 (07:55:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202407.2622.v1

Olfactory Selection Preferences of Pagiophloeus tsushimanus Adults toward Lauraceae Plants

Cong Chen, Shou Yin Li, Zhao Yan Lin, Qian Hua Ji, De Jun Hao

Subject: Biology And Life Sciences, Insect Science Keywords: Pagiophloeus tsushimanus; Cinnamomum camphora; Cinnamomum chekiangensis; Phoebe chekiangensis; host selection; olfaction

Online: 2 August 2024 (11:37:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0829.v1

Exploring Phytochemical Therapies for Long COVID: A Computational Docking Study of Phytochemicals with GPCR Targets

Pavithren Aaron, Suresh Kumar

Subject: Biology And Life Sciences, Virology Keywords: long covid; GPCR; receptor; drugs; chemokine receptors; serotonin receptors; adrenergic receptors; and histamine receptors docking; phytochemical

Online: 12 August 2024 (14:13:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1987.v1

Essential Oils as an Antifungal Alternative to Control Fusarium spp., Penicillium spp., Trichoderma spp. and Aspergillus spp.

Maritza Ruiz-Medina, Jenny Ruales

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: oregano; Origanum; rosemary; Salvia rosmarinus; clove; Syzygium aromaticum; thyme; Thymus; basil; Ocimum basilicum; cinnamon Cinnamomum

Online: 28 August 2024 (10:39:26 CEST)

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Essential oils oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus), cinnamon (Cinnamomum verum), basil (Ocimum basilicum), have antifungal properties. This study evaluated its ability to inhibit growth of isolated fungi and purified obtained from the rot of the banana peel, for the purpose of combating or inhibiting fungal growth in bananas (Musa paradisiaca). The methodology consists in preparing the dilutions to inoculate on PDA medium (Potato Dextrose Agar) amended with chloramphenicol to re-inoculate until obtaining the isolated and purified fungi of Trichoderma spp., Aspergillus spp., Penicillium spp. and Fusarium spp., which are characterized macroscopically and microscopically, subsequently, the growth is analyzed in vivo and the inhibition percentage in vitro. The results of the analysis in vivo report that the fungi in order of severity from greatest to least, was Penicillium spp., Trichoderma spp, Fusarium spp. y Aspergillus spp., while that the analysis in vivo reports that the rosemary and the basil does not inhibit fungal growth, while that the clove inhibit a 800 ppm, the cinnamon to 400 ppm and the oregano to 200 ppm to the four fungi. -2077601433541Citation: To be added by editorial staff during production. Academic Editor: Firstname Lastname Received: date Revised: date Accepted: date Published: date Copyright: © 2024 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).00Citation: To be added by editorial staff during production. Academic Editor: Firstname Lastname Received: date Revised: date Accepted: date Published: date Copyright: © 2024 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Preprint ARTICLE | doi:10.20944/preprints202403.1509.v1

Retinal Microcirculation Measurements in Response to Endurance Exercises Analysed by Adaptive Optics Retinal Camera

Maria Anna Żmijewska, Zbigniew M. Wawrzyniak, Maciej Janiszewski, Anna Zaleska-Żmijewska

Subject: Medicine And Pharmacology, Anatomy And Physiology Keywords: adaptive optics; retinal microcirculation; autoregulation; physical exercise; WRL

Online: 26 March 2024 (11:47:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202406.0265.v1

A Joint Limit Theorem for Epstein and Hurwitz Zeta-Functions

Hany Gerges, Antanas Laurinčikas, Renata Macaitienė

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: dirichlet L-function; epstein zeta-function; hurwitz zeta-function; limit theorem; probability haar measure; weak convergence

Online: 6 June 2024 (02:54:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1519.v1

A Multi-epitope Protein for High-Performance Serodiagnosis of Chronic Chagas Disease in Elisa and Lateral Flow Platforms

Evandro R. Dias, Andressa M. Durans, Barbara B. Succar, Luiz André L. T. Pinto, Guilherme C. Lechuga, Mariana G. Miguez, Janaina Figueira-Mansur, Ana Paula C. Argondizzo, Aline R. Bernardo, Rafaela L. Diniz, Gabriela S. Esteves, Edimilson D. Silva, Carlos M. Morel, José Borges-Pereira, Salvatore Giovanni De-Simone, Angela C. V. Junqueira, D. William Provance, Jr

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Chagas disease; Diagnostics; ELISA; Lateral Flow tests; Synthetic protein; T. cruzi

Online: 21 August 2024 (08:40:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2452.v1

Heterozygous Smad4 Knock out Effect on Pancreatic and Body Weight Variation in F1 Population Using Mutant and Wild Type Collaborative Cross Mice

Osayd Zohud, Iqbal M. Lone, Kareem Midlej, Aysar Nashef, Imad Abu-Elnaaj, Fuad A. Iraqi

Subject: Biology And Life Sciences, Other Keywords: Smad4; pancreatic weight; genetic background; sex differences; Collaborative Cross; F1 mice; genetic modifiers; tumor suppressor gene

Online: 30 July 2024 (10:53:56 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202403.0930.v1

Take It In: Novel Use of an Iron Bio-Absorbable Stent in Pediatric Pulmonary Stenosis

Christopher Scott, Jennifer R Maldonado, Daniel McLennan

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: - ; ; Tetralogy of Fallot
- ; ; Bio-absorbable stent
- ; ; Pediatric cardiology
- ; ; Interventional cardiology

Online: 15 March 2024 (14:37:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1151.v1

Deciphering the Role of Waxy Gene Mutations in Enhancing Rice Grain Quality: From Molecular Insights to Breeding Soft Rice Varieties

Yong Yang, Lihui Zhou, Linhao Feng, Jianying Jiang, Lichun Huang, Qing Liu, Yadong Zhang, Changquan Zhang, Qiaoquan Liu

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: soft rice; low amylose; grain transparency; Wx; eating and cooking quality

Online: 18 April 2024 (07:15:51 CEST)

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