Search | Preprints.org

Preprint ARTICLE | doi:10.20944/preprints202403.0463.v1

Diagnostic Accuracy of Saliva-Based Testing as a Vibrio cholerae Surveillance Tool

Caroline Cleopatra Chisenga, Bernard Phiri, Harriet Ng'ombe, Mutinta Muchimba, Fraser Liswaniso, Biana Bernshtein, Adam F Cunningham, David Sack, Samuel Bosomprah

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: saliva; vibriocidal antibodies; Cholera; natural infection

Online: 8 March 2024 (03:52:16 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0180.v1

The Distal Promoter of the B438L Gene of African Swine Fever Virus Is Responsible for the mRNA Transcription of the B438L and the Alternatively Spliced B169L

Hongwei Cao, Hao Deng, Yanjin Wang, Diqiu Liu, Lian-Feng Li, Meilin Li, Dingkun Peng, Jingwen Dai, Jiaqi Li, Hua-Ji Qiu, Su Li

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: African swine fever virus; B438L promoter; transcription initiation; B169L protein

Online: 3 May 2024 (09:46:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202310.0971.v1

Cardiac Abnormalities in a Predictive Mouse Model of Chagas Disease

Amanda Fortes Francisco, Giovane R. Sousa, Mhairi Vaughan, Harry Langston, Archie Khan, Shiromani Jayawardhana, Martin C. Taylor, Michael D. Lewis, John M. Kelly

Subject: Biology And Life Sciences, Parasitology Keywords: Trypanosoma cruzi; electrocardiography (ECG); Chronic Chagas Cardiomyopathy; Chagas heart disease

Online: 16 October 2023 (12:36:35 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1198.v1

Pirfenidone Prevents the Onset of Heart Fibrosis during Chronic Chagas Disease Cardiomyopathy

Tatiana Araújo Silva, Diane Thomas, Jair Lage Siqueira-Neto, Claudia Magalhaes Calvet

Subject: Biology And Life Sciences, Parasitology Keywords: Heart fibrosis; Chagas disease; Pirfenidone

Online: 20 November 2023 (07:34:46 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.1775.v1

Exploratory Study of Guanidine Derivatives as Novel Anti Trypanosoma cruzi Scaffolds

Eduardo Henrique Zampieri, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Predrag Kalaba, Alexandre Prado-Roller, Natalie Gajic, Martin Zehl, Ana Fabisikova, Luana Ribeiro dos Anjos, Luana Passianoto Gushiken, Gert Lubec, Eduardo Rene Perez Gonzalez

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Chagas disease; Trypanosoma cruzi; guanidines; in vitro trypanocidal activity; cytotoxic assays

Online: 23 July 2024 (10:00:32 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202409.0421.v1

Novel Drug-like HsrA Inhibitors Exhibit Potent Narrow-Spectrum Antimicrobial Activities against Helicobacter pylori

Javier Casado, Irene Olivan-Muro, Sonia Algarate, Eduardo Chueca, Sandra Salillas, Adrián Velázquez-Campoy, Elena Piazuelo, María F. Fillat, Javier Sancho, Ángel Lanas, Andrés González

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Helicobacter pylori; antimicrobial resistance; narrow-spectrum antibiotic; antibacterial target; response regulator; HsrA; Campylobacter jejuni

Online: 5 September 2024 (10:22:25 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202310.0829.v1

Mitochondrial DNA Haplogroups and Variants Predispose to Chagas Disease Cardiomyopathy

Frédéric Gallardo, Pauline Brochet Brochet P, David Goudenège, João Paulo Silva Nunes, Pauline Andrieux, Barbara Maria Ianni, Amanda Farage Frade, Charles Mady, Ronaldo Honorato Barros Santos, Andreia Kuramoto, Samuel Steffen, Antonio Noedir Stolf, Pablo Pomerantzeff, Alfredo Inacio Fiorelli, Edimar Alcides Bocchi, Cristina Wide Pissetti, Bruno Saba, Fabrício Dias, Marcelo Ferraz Sampaio, Fabio Antônio Gaiotto, José Antonio Marin-Neto, Abílio Fragata, Ricardo Costa Fernandes Zaniratto, Sergio Siqueira, Giselle De Lima Peixoto, Fernando Bacal, Paula Buck, Rafael Ribeiro Almeida, Hui Tzu Lin-Wang, André Schmidt, Mario Hiroyuki Hirata, Eduardo Antonio Donadi, Alexandre Costa Pereira, Virmondes Rodrigues Junior, Martino Martinelli, Michel Naslavsky, Jorge Kalil, Vincent Procaccio, Edecio Cunha-Neto, Christophe Chevillard

Subject: Biology And Life Sciences, Parasitology Keywords: chagas; cardiomyopathy; mitochondria; haplgroups; variants; copy-number

Online: 13 October 2023 (03:03:37 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1328.v1

Intranasal Trans-Sialidase Vaccine Mitigates Acute and Chronic Pathology in a Preclinical Oral Chagas Disease Model

Maria Florencia Pacini, Camila Bulfoni Balbi, Brenda Dinatale, Cecilia Farre, Paula Cacik, Florencia Belén Gonzalez, Ivan Marcipar, Ana Rosa Pérez

Subject: Public Health And Healthcare, Other Keywords: oral chagas disease; - chronic chagas cardiomyopathy - Trypanosoma cruzi; trans-sialidase - nasal vaccine - c-di-AMP

Online: 17 September 2024 (14:51:19 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1995.v1

Bacterial and Viral Co-Infections in COVID-19 Patients: Etiology and Clinical Impact

Iveta Rumenova Madzharova, Ivelina Trifonova, Neli Stoyanova Korsun, Viktoria Levterova, Petar Velikov, Silvya Voleva, Ivan Ivanov, Daniel Ivanov, Ralitsa Yordanova, Tatiana Tcherveniakova, Svetla Angelova, Iva Christova

Subject: Public Health And Healthcare, Other Keywords: SARS-CoV-2; co-infection; respiratory viruses; Haemophilus influenzae; RSV

Online: 28 August 2024 (05:13:20 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0460.v1

Proof-of-Concept of a Novel Multiepitope Recombinant Protein for the Serodiagnosis of Patients with Chagas Disease

Juliana Martins Machado, Isabela Amorim Gonçalves Pereira, Ana Clara Gontijo Maia, Mariana Ferraz Chaves Francisco, Lais Moreira Nogueira, Isadora Braga Gandra, Anna Julia Ribeiro, Kamila Alves Silva, Carlos Ananias Aparecido Resende, Jonatas Oliveira da Silva, Michelli dos Santos, Ana Alice Maia Gonçalves, Grasiele de Sousa Vieira Tavares, Miguel Angel Chávez-Fumagalli, Mariana Campos da Paz da Paz, Rodolfo Cordeiro Giunchetti, Manoel Otávio C. Rocha, Ana Thereza Chaves, Eduardo Antônio Ferraz Coelho, Alexsandro S. Galdino

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: multiepitope; recombinant protein; immunodiagnosis; Chagas disease

Online: 26 December 2022 (02:17:58 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202406.1729.v1

Left Ventricular Function and Myocardial Longitudinal Strain Analysis in Patients with Chagas Disease: Case Series and Systematic Literature Review

José Victor da Nóbrega Borges, Samira Abdel Correia Leila, Kiran Sooraj Thirukonda Jegadeesh Babu, Karma Jashvantbhai Patel, Kavya Hiteshkumar Shah, Muhammad Ahmed Shaikh, Abdul Majid Shaik, Mahitha Reddy Takkasila, Sahithi Burra

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Chagas Disease; Echocardiography; Global Longitudinal Strain

Online: 25 June 2024 (09:33:18 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202405.0203.v1

Antibiotic Treatment of Carbapenem-Resistant Acinetobacter baumannii Infections in View of the Newly Developed β-Lactams: A Narrative Review of the Existing Evidence

Francesca Serapide, Maurizio Guastalegname, Sara Palma Gullì, Rosaria Lionello, Andrea Bruni, Eugenio Garofalo, Federico Longhini, Enrico Maria Trecarichi, Alessandro Russo

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Acinetobacter baumannii; carbapenem-resistance; sulbactam-durlobactam; cefiderocol; guidelines; real-world evidence

Online: 6 May 2024 (08:47:22 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202406.0897.v1

Sterility Mosaic Disease of Pigeonpea (Cajanus cajan (L.) Huth): Current Status, Disease Management Strategies, and Future Prospects

BR Sayiprathap, Anil Kumar Patibanda, Muttappagol Mantesh, Shridhar Shivakumar Hiremath, N SAGAR, CN Lakshminarayana Reddy, CR Jahir Basha, SE Diwakar Reddy, Mediga Kasi Rao, Ramakrishnan Madhavan Nair, Hari Kishan Sudini

Subject: Biology And Life Sciences, Plant Sciences Keywords: Cajanus cajan; SMD; PPSMV; eriophyid mite; screening; disease management

Online: 13 June 2024 (07:10:51 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1151.v1

Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China

Yafang Jin, Yi Li, Shaojie Huang, Chengji Hong, Xucong Feng, Huidi Cai, Yanmei Xia, Shengkai Li, Leyi Zhang, Yongliang Lou, Wanchun Guan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Salmonella; antimicrobial resistance; pathogenicity islands; cgMLST; Whole genome sequencing

Online: 16 September 2024 (03:20:34 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202410.1054.v1

Mosquito‐Borne Disease among Individuals Experiencing Homelessness in the United States: A Literature Review

Andre Wilke, Megan Hill, Allisandra Kummer, Chalmers Vasquez, John-Paul Mutebi, Xi Huo, Han Li, Jing Chen, Shigui Ruan, John Beier, Maria Litvinova, Thomas Byrne, Marco Ajelli

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Aedes; Anopheles; Culex; chikungunya; dengue; malaria; West Nile; Zika

Online: 14 October 2024 (12:09:03 CEST)

Show abstract| Download PDF| Share

Mosquito-borne diseases are a major public health issue. The United States has experienced mosquito-borne disease outbreaks, including West Nile virus, Zika, dengue, and malaria, highlighting widespread risk across the country. In the United States, exposure to mosquito vectors primarily occurs outdoors. Individuals experiencing unsheltered homelessness are particularly vulnerable to mosquito-borne diseases due to their increased exposure to mosquito vectors, limited access to healthcare, and high prevalence of underlying conditions. This review aims to examine the literature on mosquito-borne disease infections among individuals experiencing homelessness in the United States between 1999 and 2024. Our search strategy used Boolean methods on PubMed, Scopus, and Web of Science to select relevant publications. Two queries were used: the first query focuses on individuals experiencing homelessness and mosquito-borne diseases, and the second one focuses on epidemiological analyses of mosquito-borne disease outbreaks in the US. Only studies reporting at least 1 infection among individuals experiencing homelessness were included. Our search resulted in the identification of 347 studies. After screening them, 8 were ultimately included in this review. Half of the studies identified West Nile virus among individuals experiencing homelessness and the other half reported cases of malaria. These studies show that individuals experiencing homelessness are more exposed to mosquitoes, face a higher risk of infection, and have an increased likelihood of hospitalization due to mosquito-borne diseases compared to the general population. The full extent of mosquito-borne disease exposure among individuals experiencing homelessness remains unclear. With climate change and globalization, there is a consensus in the literature showing an increasing risk of mosquito-borne disease transmission in the next decades, and we have already started to witness this increasing trend. Therefore, we argue that developing policies aimed at providing homeless populations with housing during mosquito-borne disease outbreaks could be instrumental in protecting this segment of the population and promoting health equity.

Preprint ARTICLE | doi:10.20944/preprints202410.1782.v1

Leveraging Artificial Intelligence to Identify Therapeutic Pathways in Trypanosoma cruzi: A Comparative Approach with Trypanosoma brucei

Richard Murdoch Montgomery

Subject: Computer Science And Mathematics, Mathematical And Computational Biology Keywords: Trypanosoma cruzi; Trypanosoma brucei; Chagas disease; African sleeping sickness; AI drug discovery; irreversible enzyme inhibitors; pathway modelling; drug repurposing; eflornithine

Online: 24 October 2024 (03:03:16 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1468.v1

Grapevine Trunk Diseases in Greece: Disease Incidence and Fungi Involved in Discrete Geographical Zones and Varieties

Stefanos I. Testempasis, Emmanouil A. Markakis, Georgia I. Tavlaki, Stefanos K. Soultatos, Christos Tsoukas, Danai Gkizi, Aliki K. Tzima, Epameinondas Paplomatas, Georgios S. Karaoglanidis

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Kalmusia variispora; Neosetophoma italica; Paraconiothyrium variabile; Seimatosporium vitis; meteorological conditions; wood inhabitant

Online: 23 November 2023 (05:42:59 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202303.0409.v1

Out-of-Pocket Costs Related to Chagas Disease: The Experience in Endemic Municipalities in Colombia

Rafael Herazo, Adriana Rey, Diogo Galvao, Manuel Medina Camargo, Nini Johana Cobos Pinzón, Alba Cecilia Rodriguez Sanchez, Andrea Marchiol, María Jesus Pinazo

Subject: Public Health And Healthcare, Health Policy And Services Keywords: out-of-pocket cost; primary healthcare; specialized reference hospital; Chagas

Online: 23 March 2023 (04:29:57 CET)

Show abstract| Download PDF| Share

Background Out-of-pocket costs are key expenses that many individuals incur in order to receive health services. They have been identified as a key access barrier for vulnerable populations, in particular for populations affected by neglected diseases with a chronic progression, such as Chagas disease. It is important to understand the costs of accessing healthcare services that are borne by patients with T. cruzi infection. Methodology We prepared a structured survey for patients with T. cruzi infection/Chagas disease who were all treated by the healthcare system in endemic municipalities in Colombia. The results were analyzed according to three categories: 1. the socioeconomic profiling of the patients; 2. the costs of and time spent on transportation; and 3. the opportunity costs (money that was not earned) related to treatment at the local primary care hospital or at the high-complexity reference hospital. Main findings Ninety-one patients answered the survey voluntarily. The data revealed that, when treated at the specialized reference hospital, patients spent 5.5 times more on food and accommodation, transportation costs were five times higher, and the loss of earnings was three times higher than when they were treated at the local primary care hospital. Moreover, the amount of time spent on transportation was 4 times higher at the reference hospital. Conclusions Providing comprehensive healthcare services for Chagas management at local primary healthcare hospitals would allow the most vulnerable patients to save on expenses related to medical and non-medical costs (out-of-pocket costs), in turn leading to higher adhesion to treatment thus benefiting the health system as a whole. These findings are in alignment with the WHO’s World Health Assembly 2010 Resolution on the importance of treating Chagas at local primary care hospitals, thereby saving patients time and money, allowing for timely care, and promoting access to healthcare.

Preprint ARTICLE | doi:10.20944/preprints201808.0059.v1

Inhibition of ER Stress by 2-Aminopurine Treatment Modulates Cardiomyopathy in a Murine Chronic Chagas Disease Model

Janeesh A. Plakkal, Kezia Lizardo, Sean Wang, Edward Yurkow, Jyothi F. Nagajyothi

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: chagas disease; cardiomyopathy; mitochondrial stress; endoplasmic reticulum stress; 2-aminopurine

Online: 3 August 2018 (04:36:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1348.v1

Examining the Impact of Infection Reactivation on Extracellular Vesicles purified from Patients with Chronic Chagas Disease

Rafael Pedro Madeira, Paula Meneghetti, Nicholy Lozano, Gislene M. Namiyama, Vera Lucia Pereira-Chioccola, Ana Claudia Torrecilhas

Subject: Biology And Life Sciences, Parasitology Keywords: Trypanosoma cruzi; extracellular vesicles; chronic Chagas Disease patients; immunosuppression; infection reactivation.

Online: 18 December 2023 (15:17:58 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202004.0244.v1

The Cost of Lost Productivity due to Premature Chagas Disease-Related Mortality: Evidence from Colombia (2010-2017)

Mario J. Olivera, Francisco Palencia-Sánchez, Martha Riaño-Casallas

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: Chagas disease; cost of illness; mortality premature; efficiency; organizational; life expectancy

Online: 15 April 2020 (10:18:15 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0735.v1

Effects of Adding Sphingomonas Z392 to Drinking Water on Growth Performance, Intestinal Histological Structure and Microbial Community of Broiler Chickens

Mingcheng Wang, Jie Zhong, Yanan Guo, Shuqiang Zhao, Huili Xia, Gailing Wang, Chaoying Liu, Aizhen Guo

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Broiler; Sphingomonas; EPI; Intestinal histology; Intestinal microbiota

Online: 12 May 2024 (07:44:22 CEST)

Show abstract| Download PDF| Share

Probiotics have emerged as a prominent alternative to antibiotics in antimicrobial-free broiler farming. To assess the effect of the probiotics Sphingomonas sp. Z392 isolated and identified by this this laboratory on broiler growth, we conducted a study to examine the changes in broiler body weight, EPI, intestinal histological structure and gut microbiota. The results showed that supplementation of broiler drinking water with 4 × 105 CFU/mL of Sphingomonas resulted in an increase in the relative abundance of Lactobacillus, Bacteroides, Lachnospiraceae, Aminobacterium, Oribacterium, Christensenellaceae, Faecalibacterium, Barnesiella, Ruminococcus, Parabacteroides, Phascolarctobacterium, Butyricicoccaceae, and Caproiciproducens, which were reported to be positively correlated with the digestion and absorption of broiler chickens. The relative abundance was increased of Odoribacter, Alistipes, Parabacteroides, and Rikenellaceae, which were reported to be negatively correlated with the occurrence of intestinal diseases. The relative abundance was decreased of Campylobacter, Shigella Castellani, Bilophila, Campylobacter, Clostridia, and Anaerotruncus, which were reported positively correlated with the occurrence of intestinal diseases. At the same time, it was increased in the integrity of small intestinal villus morphology, the number of goblet cells in small intestinal epithelial cells, the health of mitochondria in the cytoplasm of jejunal villous epithelial cells, and the number of lysosomes in the cytoplasm of goblet cells in small intestinal epithelium, ileal villous epithelial cells, mitochondria in the cytoplasm of large intestinal villous epithelial cells, the VH/CD of the ileum, digestive, absorption, and defense capabilities. Especially the final weight was increased by 4.33% and the EPI increased by 10.10%. Therefore, the supplementation of broiler drinking water with Sphingomonas generated better economic benefits for the broiler chickens.

Preprint ARTICLE | doi:10.20944/preprints202408.1519.v1

A Multi-epitope Protein for High-Performance Serodiagnosis of Chronic Chagas Disease in Elisa and Lateral Flow Platforms

Evandro R. Dias, Andressa M. Durans, Barbara B. Succar, Luiz André L. T. Pinto, Guilherme C. Lechuga, Mariana G. Miguez, Janaina Figueira-Mansur, Ana Paula C. Argondizzo, Aline R. Bernardo, Rafaela L. Diniz, Gabriela S. Esteves, Edimilson D. Silva, Carlos M. Morel, José Borges-Pereira, Salvatore Giovanni De-Simone, Angela C. V. Junqueira, D. William Provance, Jr

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Chagas disease; Diagnostics; ELISA; Lateral Flow tests; Synthetic protein; T. cruzi

Online: 21 August 2024 (08:40:17 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1563.v1

Isolation and Identification of Alternaria alternata from Potato Leaf Spot Disease in Korea: Selection of Effective Fungicides

Jiyoon Park, Seoyeon Kim, Miju Jo, Sunmin An, Youngjun Kim, Jonghan Yoon, Min-Hye Jeong, Eun Young Kim, Jaehyuk Choi, Yangseon Kim, Sook-Young Park

Subject: Biology And Life Sciences, Life Sciences Keywords: Alternaria alternata; brown spot disease; pathogenicity test; phylogenetic analysis; Solanum tuberosum L.

Online: 21 December 2023 (05:58:41 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202401.1321.v1

Examining Natural Products with the Potential to Chagas Disease: An In-depth Analysis

Eze Elijah Ajaegbu, Chizurum Philip Ikegbuna

Subject: Medicine And Pharmacology, Pharmacy Keywords: Chagas disease; Trypanosoma cruzi; Natural products; Therapeutic approaches; Anti-parasitic compounds; Plant-derived compounds

Online: 17 January 2024 (12:04:26 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202408.0118.v1

Stress Factors Affecting the Development of Vairimorpha (Nosema) spp. Disease in Bee Colonies

Beáta Sabolová, Petra Kandráčová, Monika Sučik

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Vairimorpha spp.; diagnostic; duplex PCR; Slovakia ;

Online: 2 August 2024 (03:48:35 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202403.1641.v1

Global Distribution of Canine Visceral Leishmaniasis and the Role of the Dog in the Epidemiology of the Disease

Diego Fernandes Vilas-Boas, Eiji Kevin Nakasone Nakasone, Ana Alice Maia Gonçalves, Daniel Ferreira Lair, Diana Souza de Oliveira, Diogo Fonseca Soares Pereira, Geralda Gabriele Silva, Ingrid dos Santos Soares Conrado, Lucilene Aparecida Resende, Maykelin Fuentes Zaldívar, Reysla Maria da Silveira Mariano, Walderez Ornelas Dutra, Miguel Angel Chávez Fumagalli, Alexsandro Sobreira Galdino, Denise Silveira-Lemos, Rodolfo Cordeiro Giunchetti

Subject: Biology And Life Sciences, Parasitology Keywords: Leishmania infantum; Epidemiology; Canine Visceral Leishmaniasis

Online: 27 March 2024 (06:39:20 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202309.0207.v1

The Potential Use of Peptides in the Fight against Chagas Disease and Leishmaniasis

Nir Qvit, Mulate Zerihun, Mahesh Kumar, Hayelom Berhe

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Neglected tropic diseases; Parasites; Chagas disease; Leishmaniasis; Life cycle; Drug target; Biochemical mechanisms; Pharmacological therapeutics; Peptides

Online: 6 September 2023 (14:52:33 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.0297.v1

The Autophagy-Related Musa acuminata Protein MaATG8F Interacts with MaATG4B, Regulating Banana Disease Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

Huoqing Huang, Yuzhen Tian, Yile Huo, Yushan Liu, Wenlong Yang, Yuqing Li, Mengxia Zhuo, Dandan Xiang, Chunyu Li, Ganjun Yi, Siwen Liu

Subject: Biology And Life Sciences, Horticulture Keywords: ATG8; ATG4; autophagy; Fusarium oxysporum f. sp. cubense tropical race 4; plant disease resistance

Online: 6 December 2023 (03:17:36 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202402.1176.v1

Unmasking the Mechanism behind Miltefosine: Revealing the Disruption of Intracellular Ca2+ Homeostasis as a Rational Therapeutic Target in Leishmaniasis and Chagas Disease

Gustavo Benaim, Alberto Paniz-Mondolfi

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Ca2+ signaling; Ca2+ regulation; miltefosine; acidocalcisome; mitochondria; Ca2+ Channel; sphingosine; Chagas Disease; Leishmaniasis

Online: 21 February 2024 (04:22:08 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.2103.v1

Bifidobacterium infantis and Bifidobacterium breve Improve Symptomatology and Neuronal Damage in Neurodegenerative Disease. A Systematic Review.

Manuel Reiriz, Ana Isabel Beltrán-Velasco, Víctor Echeverry-Alzate, Esther Martínez-Miguel, Silvia Gómez-Senent, Sara Uceda, Vicente Javier Clemente-Suárez

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: neurodegenerative disease; probiotics; Alzheimer´; s disease; Parkinson´; s disease; neurodegenerative process; Bifidobacterium infantis; Bifidobacterium longum subsp. infantis; Bifidobacterium breve

Online: 31 May 2024 (08:10:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1998.v1

Detection of Apple Proliferation Disease by Hyperspectral Sensors and Machine Learning Based Image Analysis

Uwe Knauer, Sebastian Warnemünde, Patrick Menz, Bonito Steffen Thielert, Lauritz Klein, Katharina Holstein, Miriam Runne, Wolfgang Jarausch

Subject: Biology And Life Sciences, Plant Sciences Keywords: hyperspectral; random forest; machine learning; apple; ‘ Candidatus Phytoplasma mali’ ; disease monitoring

Online: 25 September 2024 (12:16:21 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202405.0087.v1

Current Treatment Approaches to Eliminate Borrelia burgdorferi and Potential Strategies to Alleviate Persistent Lyme Disease Symptoms

Kashaf Zafar, Onyedikachi Azuama, Nikhat Parveen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Lyme disease; Borrelia burgdorferi; Treatment; antimicrobials; Post-treatment Lyme disease syndrome; PTLDS

Online: 2 May 2024 (12:03:42 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202404.1333.v1

Contrasting Disease Progression, Microglia Reactivity, Tolerance, and Resistance to Toxoplasma gondii Infection in Two Mouse Strains

Daniel Guerreiro Diniz, Jhonnathan Henrique Palheta de Oliveira, Luma Cristina Ferreira Guerreiro, Gabriel Castro de Menezes, Alexa Camila Lopes de Assis, Tainá Quintella Duarte, Izabelly Biase Damasceno dos Santos, Flávia Domingues Maciel, Gabrielly Lisboa da Silva Soares, Sanderson Corrêa Araújo, Felipe Tuji de Castro Franco, Ediclei Lima do Carmo, Rafaela dos Anjos Pinheiro Bogoevich Morais, Camila Mendes de Lima, Dora Brites, Daniel Clive Anthony, José Antonio Picanço Diniz, Cristovam Wanderley Picanço-Diniz

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Toxoplasma gondii; ; tolerance; resistance; ocular infection; microglia; dentate gyrus; sickness behavior

Online: 22 April 2024 (08:41:09 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202312.2135.v1

Influence of the Hla-Cw6 Allele and IFIH1/MDA5 Gene Variants on the Cardiometabolic Risk Profile of Patients with Psoriatic Disease

Rubén Queiro

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: HLA-Cw6; IFIH1/MDA5 gene; psoriatic arthritis; psoriasis; cardiovascular risk

Online: 28 December 2023 (04:52:00 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.2225.v1

The Extract of Larrea tridentata Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in Penaeus vannamei against Vibrio spp, Causing Acute Hepatopancreatic Necrosis Disease

Germán León-Valdez, Wenceslao Valenzuela-Quiñonez, Píndaro Álvarez-Ruiz, C. A. Soto-Robles, Eusebio Nava-Perez, Gabriela López-Cervantes, Magnolia Montoya-Mejía

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Pacific white shrimp; Vibrio; green synthesis; AHPND; MIC

Online: 27 September 2024 (13:44:06 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202408.2122.v1

Essential Oils as an Antifungal Alternative to Control Cladosporium spp., Lasiodiplodia spp., Colletotrichum spp., Fusarium spp. and Aspergillus spp. Isolated of Musa paradisiaca

Ruiz-Medina Maritza D., Jenny Ruales

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Origanum vulgare; Salvia rosmarinus; Syzygium aromaticum; Thymus; Ocimum basilicum; Cinnamomum

Online: 29 August 2024 (08:28:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1258.v1

Sanger Sequencing Revealed Borrelia burgdorferi with FlaB Paralogs Causing Spirochetemia in Early Localized Lyme Disease

Sin Hang Lee

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: sanger sequencing; spirochetemia; early localized Lyme disease; FlaB paralogs; SNP in FlaB gene; single Borrelia burgdorferi cell; platelet-rich plasma

Online: 17 September 2024 (08:10:09 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.0534.v1

Using Acanthamoeba spp. as a Cell Model to Evaluate Leishmania Infections

Helena Santos, Gabriella Linhares, Rhagner Bonono do Reis, Igor Cardoso, Claudia Masini d'Avila, Vitor Ennes Vidal

Subject: Biology And Life Sciences, Parasitology Keywords: Acanthamoeba castellanii, Acanthamoeba polyphaga, Leishmania, interaction host-parasite

Online: 5 July 2024 (12:20:28 CEST)

Show abstract| Download PDF| Share

Leishmaniasis represents a severe global health problem. In the last decades, there have been significant challenges in controlling this disease due to the unavailability of licensed vaccines, the high toxicity of the available drugs, and an unrestrained surge of drug-resistant parasites, and human immunodeficiency virus (HIV)–Leishmania co-infections. Leishmania spp. preferentially invade macrophage lineage cells of vertebrates for replication after subverting cellular functions of humans and other mammals. These early events in host–parasite interactions are likely to influence the future course of the disease. Thus, there is a continuing need to discover a simple cellular model that reproduces the in vivo pathogenesis. Acanthamoeba spp. are non-mammalian phagocytic amoeba with remarkable similarity to the cellular and functional aspects of macrophages. We aimed to assess whether the similarity reported between macrophages and Acanthamoeba spp. is sufficient to reproduce the infectivity of Leishmania spp. Herein, we analyzed co-cultures of Acanthamoeba castellanii or Acanthamoeba polyphaga with Leishmania infantum, Leishmania amazonensis, Leishmania major, and Leishmania braziliensis. Light and fluorescence microscopy revealed that the flagellated promastigotes attach to the A. castellanii and/or A. polyphaga in a bipolar and or random manner, which initiates their uptake via pseudopods. Once inside the cells, the promastigotes undergo significant changes, which result in the obligatory amastigote-like intracellular form. There was a productive infection with a continuous increase in intracellular parasites. However, we frequently observed intracellular amastigotes in vacuoles, phagolysosomes, and the cytosol of Acanthamoeba spp. Our findings corroborate that Leishmania spp. infects Acanthamoeba spp. and replicates in them but does not cause their rapid degeneration or lysis. Overall, the evidence presented here confirms that Acanthamoeba spp. have all prerequisites and can help elucidate how Leishmania spp. infect mammalian cells. Future work exposing the mechanisms of these interactions should yield novel insights into how these pathogens exploit amoebae.

Preprint ARTICLE | doi:10.20944/preprints202401.2052.v1

The Info-Geometric Analysis of Wind Speed Dynamics and How Energy Is Influentially Impacted by Information Geometry (IG)

Dr Ismail A Mageed

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Wind Speed Manifold (WSM), IG,GeodesicEquations (GEs)

Online: 30 January 2024 (08:40:24 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0730.v2

Cloning and Mutagenesis of AmpC β–lactamase in Pseudomonas aeruginosa for the Identification of Targetable Allosteric Sites

Shriya Bhat, Stephen Lory

Subject: Immunology And Microbiology, Biology And Life Sciences Keywords: Pseudomonas aeruginos; &beta; – lactamase; ampC

Online: 25 October 2024 (11:28:42 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202404.0922.v1

Mortality of Boreal Trees

Petri P. Kärenlampi

Subject: Biology And Life Sciences, Forestry Keywords: Picea abies; Pinus sylvestris; Betula pendula; Betula pubescens; logistic regression

Online: 15 April 2024 (10:19:42 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202402.1026.v1

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

Omar E. Ahuatzin-Flores, Eduardo Torres, Edith Chávez-Bravo

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Acinetobacter baumannii; multi-resistance; natural habitats; reservoirs

Online: 19 February 2024 (08:19:03 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202403.0023.v1

Co-occurrence of Borrelia burgdorferi sensu lato and Babesia spp. DNA in Ixodes ricinus Ticks Collected from Vegetation and Pets in the City of Poznań, Poland

Justyna Liberska, Jerzy Michalik, Julia Olechnowicz, Miroslawa Dabert

Subject: Biology And Life Sciences, Parasitology Keywords: Ixodes ricinus; Borrelia burgdorferi s.l.; Babesia microti; Babesia venatorum; Babesia canis; Borrelia miyamotoi; coinfections, babesiosis, urban green areas

Online: 1 March 2024 (05:42:09 CET)

Show abstract| Download PDF| Supplementary Files| Share

Green spaces localized in cities create favourable environmental conditions for Ixodes ricinus, the most widespread and important vector of tick-borne pathogens in Central Europe. In this study, we described the prevalence of Borrelia burgdorferi s.l. and Babesia species found in mono- and double infections among I. ricinus ticks occurring in urban areas of the city of Poznań, west-central Poland. Two tick groups were examined: (i) a group of 1,029 host-seeking ticks (460 nymphs, 289 females, and 280 males and (ii) a group of 1,268 engorged female ticks removed from 1,115 tick-infested animals, dogs and cats. The overall prevalence of B. burgdorferi s.l. found in host-seeking ticks was almost two-fold higher in comparison to ticks derived from pets (8.7 vs. 4.7%, respectively, χ2 test, P = 0.001). Borrelia afzelii and B. garinii were the most prevalent species both in ticks from vegetation (3.7% and 3.7%, respectively) and from pets (3.7% and 0.6%, respectively). Babesia infections were slightly more common in feeding ticks compared to those from vegetation (6.0 vs. 4.7%, respectively), however, the difference was not significant. Three Babesia species including Ba. microti, Ba. venatorum and Ba. canis were identified. Babesia canis and Ba. microti were the most prevalent in host-seeking (2.6% and 1.4%, respectively) and feeding ticks (2.8% and 2.2%, respectively), whereas Ba. venatorum was the rarest piroplasm. All Ba microti obtained sequences (n=42) were identical to Ba. microti genotype "Jena/Germany" that is considered as pathogenic to humans. Furthermore, the sequences of Ba. venatorum (n=17) were identical with those isolated from patients in Italy, Austria and Poland. Altogether, out of 121 Babesia-positive ticks, 19.8% were simultaneously infected with B. burgdorferi s.l. spirochetes. We found similar prevalences of co-infections in host-seeking (1.1%) as well as in feeding ticks (1.0%). In the first group, Ba. canis prevailed in coinfections (73%), mostly with B. afzelii and B. garinii, in the second group, Babesia microti dominated with B. afzelii (92.7%). This is the first finding documenting the co-occurrence of both pathogens in ticks from companion animals. The detection of Ba. microti and Ba. venatorum sequences identical to strains pathogenic to humans as well as predominance of LB spirochetes, B. afzelii and B. garinii in I. ricinus from vegetation and animals indicates that there is a potential risk of acquiring human babesiosis and/or Lyme borreliosis in urban areas of the city of Poznań. Although, the overall risk of encountering a Ba. microti and B. burgdorferi s.l. co-infected tick was low (1.0%), the potential for more severe clinical symptoms and complications deserves notice. A relatively high infection rates of Ba. canis in dog-derived ticks (3.2%) and in host-seeking ticks (2.6%), suggests that I. ricinus could potentially be involved in the circulation of this piroplasm in areas, where the competent vector, Dermacentor reticulatus is absent or rare.

Preprint ARTICLE | doi:10.20944/preprints202407.2622.v1

Olfactory Selection Preferences of Pagiophloeus tsushimanus Adults toward Lauraceae Plants

Cong Chen, Shou Yin Li, Zhao Yan Lin, Qian Hua Ji, De Jun Hao

Subject: Biology And Life Sciences, Insect Science Keywords: Pagiophloeus tsushimanus; Cinnamomum camphora; Cinnamomum chekiangensis; Phoebe chekiangensis; host selection; olfaction

Online: 2 August 2024 (11:37:49 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.1277.v1

Antibiotic Resistance Pattern in Intensive Care Unit Patients of Bangladesh

Md. Tahsin Salam, Kaniz Fatema Bari, Dewan Muhammad Mahmudul Gafur, Md. Omar Faruk, Khurshida Akter, Tanim Ahmed shaikat, Ashik Chandra Das, Mr Tofayel, Mahmudul Hasan Mithun, Atiqur Rahman Sunny

Subject: Biology And Life Sciences, Life Sciences Keywords: Resistance to antibiotics; ICU; Pneumonia.; Diabetes; Infection; Bangladesh

Online: 17 January 2024 (08:26:15 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.0771.v1

Predicting Autonomous Driving Behavior through Human Factor Considerations in Safety-Critical Events

Jamal Raiyn, Galia Weidl

Subject: Engineering, Transportation Science And Technology Keywords: car– following; non-monotonic logic; driving behavior; naturalistic driving studies;
safety-critical events; cognitive vehicles

Online: 12 December 2023 (04:16:31 CET)

Show abstract| Download PDF| Share

This research explores the predictive capabilities of autonomous driving systems by integrating human factor considerations within the context of safety-critical events. Recognizing the significance of human behaviors in influencing driving dynamics, the study employs advanced modeling techniques to enhance the accuracy of predictions in scenarios that demand heightened safety measures. Traditional rule-based systems and monotonic logic often fall short in addressing the complexities of safety-critical events. To overcome these limitations, the research proposes the application of non-monotonic logic, allowing for flexible and adaptive reasoning that accommodates exceptions and context-specific information.The study emphasizes the importance of incorporating individual differences among drivers, such as risk-taking tendencies, reaction times, decision-making processes, and driving styles. By considering these human factors, the research aims to develop realistic and accurate autonomous driving models that capture the nuances of real-world driving scenarios, especially in safety-critical situations. The predictive model takes into account both internal and external factors, enabling the autonomous system to anticipate and respond effectively to unforeseen events.The primary goal is to provide autonomous vehicles with the capability to make plausible inferences, handle conflicting data, and adapt their behavior in real-time during safety-critical events. The proposed model integrates personalized cognitive agents for each driver, incorporating their unique preferences, characteristics, and needs. This personalized approach aims to optimize the safety and efficiency of autonomous driving, contributing to the ongoing evolution of intelligent transportation systems.In conclusion, this research contributes to advancing the field of autonomous driving by introducing a predictive model that leverages human factor considerations to enhance safety in safety-critical events. The incorporation of non-monotonic logic and individualized cognitive agents signifies a comprehensive approach to address the challenges associated with predicting autonomous driving behavior, paving the way for safer and more reliable autonomous vehicles in dynamic and unpredictable environments.

Preprint ARTICLE | doi:10.20944/preprints202402.1092.v1

Characteristics of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in the Dog Population

Zuzanna Czekaj, Malgorzata Dorota Klimowicz-Bodys, Magdalena Karwanska, Magdalena Siedlecka, Krzysztof Rypula

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Capnocytophaga canimorsus; Capnocytophaga cynodegmi; Capnocytophaga spp.; dog; molecular diagnostic

Online: 20 February 2024 (08:51:15 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0222.v1

Frankincense (Boswellia serrata) Extract Effects on the Growth, Biofilm Formation, and Intracellular Infection of Porphyromonas gingivalis and Fusobacterium nucleatum in Human Gingival Epithelial Cells

David Vang, Aline Cristina de Abreu Moreira-Souza, Nicholas Zusman, German Moncada, Harmony Matshik Dakafay, Homayon Asadi, David M. Ojcius, Cassio Luiz Coutinho Almeida-da-Silva

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Porphyromonas gingivalis; Fusobacterium nucleatum; Boswellia serrata; biofilm; epithelial cells

Online: 5 February 2024 (05:37:41 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1304.v1

Alternaria alternata and A.koreana, the Causal Agent of a New Leaf Spot in Celtis sinensis and Their Sensitivity to Fungicides

Qiuqin Wang, Xiu-Yu Zhang, Yu Wan, Yin-Juan Zhao

Subject: Environmental And Earth Sciences, Other Keywords: Celtis sinensis; Alternaria alternata; Alternaria koreana; fungi; identification; fungicides

Online: 21 November 2023 (09:39:54 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202402.1122.v1

How Satellite Imaging and Deep Learning Are Influenced by Tensor Decompositions: A Review

Ismail A. Mageed

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: Tensor Decompositions (TDs); Satellite Imaging (SI); Deep Learning (DL); Tensor Train Networks (TTNs); Tucker decomposition (TUD); canonical polyadic decomposition (CPD); polyadic decomposition (PD); Roll, Pitch, and Yaw (RPY)

Online: 20 February 2024 (09:08:09 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202306.1620.v1

The Therapeutic Potential of Angeli’s Salt in Mitigating Acute Trypanosoma Cruzi Infection in Mice

Vera Lúcia Hideko Tatakihara, Aparecida Donizette Malvezi, Rito Santo Pereira, Bruno Fernando Cruz Lucchetti, Lucas Felipe Dos Santos, Rubens Cecchini, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta, Katrina M Miranda, Waldiceu Aparecido Verri Jr, Marli Cardoso Martins-Pinge, Phileno Pinge-Filho

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Chagas disease; Therapy; Nitroxyl; Leukopenia; Thrombocytopenia; Oxidative stress; Macrophages

Online: 22 June 2023 (12:17:36 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202302.0054.v1

The Elusive Trypanosoma cruzi Disperse Gene Protein Family (DGF-1)

Jose Luis Ramirez

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Chagas Disease; surface proteins; disperse protein family; Trypanosoma cruzi

Online: 3 February 2023 (03:11:08 CET)

Show abstract| Download PDF| Share

Preprint BRIEF REPORT | doi:10.20944/preprints201910.0166.v1

In Vitro Benznidazole and Nifurtimox Susceptibility Profile of Trypanosoma cruzi Strains Belonging to Discrete Typing Units TcI, TcII and TcV

Suzana Revollo, Bruno Oury, Andrea Vela, Michel Tibayrenc, Denis Sereno

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Chagas Disease; Trypanosoma cruzi; benznidazole; nifurtimox; antimicrobial susceptibility test

Online: 15 October 2019 (08:26:29 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1280.v1

Effects of Three Yeast Strains on In Vitro Rumen Fermentation of Corn Stover and a Total Mixed Ration

Bexy Gonzalez-Mora, Francisco Castillo-Rangel, Joel Dominguez-Viveros, Perla Lucia Ordoñez-Baquera, Michael E. Hume, Robin C. Anderson, Monserrath Félix-Portillo, Agustin Corral-Luna, Jaime Salinas-Chavira, Yamicela Castillo-Castillo, Oscar Ruiz-Barrera

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: microbial feed additives; probiotic; Candida norvegensis; Pichia guilliermondii; Saccharomyces cerevisiae

Online: 19 August 2024 (05:49:25 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.1685.v1

Nutritive Value and Degradation Kinetic Parameters of Three Plants for Feeding Bradypus variegatus Schinz

Igor Luiz Carvalho Máximo, Júlio Cézar dos Santos Nascimento, Gilcifran Prestes de Andrade, José Lypson Pinto Simões Izidro, Priscilla Virgínio de Albuquerque, Daniel Bezerra do Nascimento, Janerson José Coêlho, Marleyne José Afonso Accioly Lins Amorim, Alexandre Carneiro Leão de Mello, Romero Marcos Pedrosa Brandão Costa, Carlos Bôa-Viagem Rabello, Maria do Carmo Mohaupt Marques Ludke, Apolônio Gomes Ribeiro, Ricardo Alexandre Silva Pessoa

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: bradypodidae; chemical composition; Inga sp.; in vitro kinetics; Pterodon sp.

Online: 22 July 2024 (07:16:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0152.v1

Effects of Sand Burial Depth on Bivalve Species Predation by the Invasive Blue Crab, Callinectes sapidus

Patricia Prado, Ignasi Gairin, Silvia Falco

Subject: Biology And Life Sciences, Aquatic Science Keywords: Ruditapes spp.; Cerastoderma glaucum; Ensis siliqua; predation escape; Ebro Delta

Online: 3 May 2024 (07:20:17 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.0529.v1

Detection of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Chikwanda Chileshe, Misheck Shawa, Nelson Phiri, Joseph Ndebe, Sipho Khumalo, Chie Nakajima, Kajihara Masahiro, Hideaki Higasi, Hirofumi Sawa, Yasuhiko Suzuki, Walter Muleya, Bernard Hang'ombe

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Enterobacteriaceae, bla_CTX-M, bla_TEM

Online: 7 December 2023 (13:52:04 CET)

Show abstract| Download PDF| Supplementary Files| Share

: Poultry products in Zambia form an integral part of the human diet in many households, as they are cheap and easy to produce. The burden of poultry diseases has, however, remained a major challenge. Growing consumer demand for poultry products in Zambia has resulted in non-prudent antimicrobial use on farms, intending to prevent and treat poultry diseases for growth optimization and maximising profits. This cross-sectional study aimed to identify the different types of bacteria causing diseases in chickens in Lusaka and to detect the extended-spectrum-lactamase (ESBL)-encoding genes. We collected 215 samples from 91 diseased chickens at three post-mortem facilities and screened them for gram-negative bacteria. Of these samples, 103 tested positive for various clinically relevant Enterobacteriaceae, including Enterobacter (43/103, 41.7%), Escherichia coli (20/103, 19.4%), Salmonella (10/103, 9.7%), and Shigella (8/103, 7.8%). Other isolated bacteria included Yersinia, Morganella, Proteus, and Klebsiella, which accounted for 21.4%. E. coli, Enterobacter, Salmonella, and Shigella were subjected to antimicrobial susceptibility testing. The results revealed that E. coli, Enterobacter, and shigella were highly resistant to tetracycline, ampicillin, amoxicillin, and trimethoprim-sulfamethoxazole, while Salmonella showed complete susceptibility to all antibiotics. The observed resistance patterns correlated with antimicrobial usage estimated from sales data from a large-scale wholesale and retail company. Six (6/14, 42.9%) E. coli isolates tested positive for bla_CTX-M, whilst eight (8/14, 57.1%) Enterobacter samples tested positive for bla_TEM. Interestingly, four (4/6, 66.7%) of the E. coli isolates carrying bla_CTX-M-positive strains were also positive for bla_TEM. Sanger sequencing of the PCR products revealed that five (5/6,83.3%) of the abovementioned isolates possessed the bla_CTX-M-15 allele. The results suggest the presence of potentially pathogenic ESBL-producing Enterobacteriaceae in poultry, threatening public health. Keywords:. Enterobacteriaceae, bla_CTX-M, bla_TEM

Preprint ARTICLE | doi:10.20944/preprints202405.1831.v1

Crude Extracts from Lippia adoensis (Hochst.) Inhibit the Growth of Plasmodium, Leishmania and Trypanosoma Parasites

Eugenie Aimée Madiesse Kemgne, Mariscal Brice Tchatat Tali, Darline Dize, Cyrille Armel Njanpa Ngansop, Boniface Pone Kamdem, Fabrice Fekam Boyom

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Lippia adoensis; Plasmodium falciparum; Leishmania donovani; Trypanosoma brucei; molecular networking; LC-MS analysis

Online: 28 May 2024 (11:28:34 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202409.0405.v1

The Maturation of the International Health Crisis Response: the Polish Typhus Epidemic of 1916-1923 Compared to the African Ebola Virus Disease Epidemic of 2013-2016: Part I, The Polish Epidemic

Gregory M Anstead

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: epidemic typhus; relapsing fever; Poland; international health; Rickettsia prowazekii; human body louse; Ebola Virus Disease

Online: 9 September 2024 (04:23:53 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202310.1928.v2

Association of a Global Invasive Pest Spodoptera frugiperda (Lepidoptera: Noctuidae) with the Local Parasitoids: Prospects for a New Approach in Biological Control

Ihsan Nurkomar, Ichsan Luqmana Indra Putra, Damayanti Buchori, Fajar Setiawan

Subject: Biology And Life Sciences, Insect Science Keywords: Brachymeria; Eupelmus; host-parasite interaction; local adaptation; Platygasteridae; Telenomus remus; Stenobracon

Online: 30 January 2024 (04:59:24 CET)

Show abstract| Download PDF| Supplementary Files| Share

Spodopotera frugiperda is a worldwide invasive pest that has caused major economic damage. According to classical biological control theory, natural enemies that can control invasive pests come from the area of origin as the pests that have gone through coadaptation processes. However, the new association’s approach suggests that local natural enemies are equally capable to control invasive pests. Due to the lack of data on the association of S. frugiperda and local natural enemies, research was conducted through a rapid survey to study the population density of S. frugiperda in Yogyakarta, Indonesia and to study the diversity of parasitoid associated with S. frugiperda. The results showed the difference in S. frugiperda population and parasitism rate, with 15 parasitoid species associated with S. frugiperda. Four egg parasitoids, eight larval parasitoids, and three pupal parasitoids were found to be associated with S. frugiperda for three years after it was first discovered in Indonesia. A new association was found between S. frugiperda and three parasitoid species, consisting of two egg parasitoids (Platygasteridaesp.01 and Platygasteridaesp.02) and a larval parasitoid (Eupelmidaesp.01). A relatively new association was found between S. frugiperda and two larval parasitoids (Coccygidium sp. and Stenobracon sp.), and an existing association was found between seven species including an egg parasitoid Telenomus remus, three larval parasitoids (Cotesia sp., Microplitis sp., Campoletis sp.), and three pupal parasitoids (Brachymeria lasus, B. femorata, and Charops sp.). Telenomus remus is the most dominant parasitoid, with a higher abundance and parasitism rate. This study also reported the first findings of the association of S. frugiperda with the larval parasitoid Megaselia scalaris in Indonesia. The result suggests another method for biological control using the new association approach since local natural enemies can foster quick adaptation to invasive pests.

Preprint ARTICLE | doi:10.20944/preprints202409.1066.v1

Nitrogen Assimilation, Biomass and Yield in Response to Application of Algal Extracts, Rhizobium sp. and Trichoderma asperellum, as Biofertilizers in Hybrid Maize

Sandra Pérez-Álvarez, Erick H. Ochoa-Chaparro, Julio César Anchondo-Páez, César M. Escobedo-Bonilla, Joel Rascón-Solano, Marco A. Magallanes-Tapia, Luisa Patricia Uranga-Valencia, Reinier Hernández-Campos, Esteban Sánchez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: algal extracts; biofertilizers; nitrogen assimilation; Rhizobium sp.; Trichoderma asperellum; Zea mays L.

Online: 13 September 2024 (08:46:08 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.0670.v1

A Multifaceted Exploration of the Biological Properties of Three Amazonian Fruit Seeds

Claudia Cristina Pérez Jaramillo, Jonh Jairo Méndez Arteaga, Liceth N. Cuéllar Álvarez, Walter Murillo Arango

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Eugenia stipitata; Euterpe oleracea; Plukenetia volubilis; non-communicable diseases; bioactive compounds; byproducts

Online: 9 July 2024 (07:45:57 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1671.v1

Combination Therapy for Multi-Drug-Resistant Mycoplasma genitalium

Chris Kenyon

Subject: Medicine And Pharmacology, Internal Medicine Keywords: M. genitalium; minocycline; metronidazole; methenamine; pristinamycin; AMR

Online: 24 September 2024 (03:49:18 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.1714.v1

Candida tropicalis critically affects Candida albicans virulence by limiting its capacity to adhere to the host intestinal surface leading to decreased susceptibility to colitis in mice

Kyle Roberts, Abdullah Osme, Carlo De Salvo, Eleonora Zoli, Janet Herrada, Thomas McCormick, Mahmoud Ghannoum, Fabio Cominelli, Luca Di Martino

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: C. albicans; colitis; C. tropicalis; mycobiome; biofilm

Online: 29 February 2024 (10:53:24 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1588.v1

Microbiome Dynamics of Salmo salar Skin and Gill

Marcos Godoy, Yoandy Coca, Rudy Suárez, Marco Montes de Oca, Jacob W. Bledsoe, Ian Burbulis, Diego Caro, Juan Pablo Pontigo, Vinicius Maracaja-Coutinho, Raúl Arias-Carrasco, Leonardo Rodríguez-Córdova, César Sáez-Navarrete

Subject: Biology And Life Sciences, Aquatic Science Keywords: Piscirickettsia salmonis; Salmo salar; skin; ulcer; microbiome

Online: 24 November 2023 (09:25:08 CET)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202407.1689.v1

An Alternative Method to Synthesize N,2,3-Trimethyl-2H-indazol-6-amine as a Key Component in the Preparation of Pazopanib

Thi Thanh Cham Bui, Hue Linh Luu, Thi Thanh Luong, Thi Ngoc Nguyen, Huy Duc Ngo, Nguyet Suong Huyen Dao, Van Giang Nguyen, Dinh Luyen Nguyen, Nguyen Trieu Trinh, Van Hai Nguyen

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: -methyl-6-nitro-1H-indazole; pazopanib; N,N,2,3-tetramethyl-2H-indazol-6-amine; N,2,3-trimethyl-2H-indazol-6-amine

Online: 22 July 2024 (08:06:19 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1231.v1

Antioxidant and Antibacterial Activities of Selected Medicinal Plants from Addis Ababa against MDR-Uropathogenic Bacteria

Mebrahtu Eyasu, Juana Benedí, José Antonio Romero, Sagrario Martín-Aragón

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Thymus schimperi; Rhamnus prinoides; Justicia shimperiana; multidrug resistance; Escherichia coli; Klebsiella pneumoniae ESBL

Online: 16 August 2024 (10:11:07 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1652.v1

Bacterial Community in Fresh Fruits and Vegetables Sold in Streets and Open-Air Markets of Dakar, Senegal

Sarbanding Sane, Sophie D. Tene, Abou Abadallah Malick Diouara, Seynabou Coundoul, Malick Mbengue, Yakhya Dieye

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Food safety; Fresh fruits and vegetables; E. coli; Vibrio spp; Salmonella spp; metagenomic

Online: 27 November 2023 (11:11:22 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202311.1060.v1

Dermatophyte Infections Worldwide: Increase in Incidence and Associated Antifungal Resistance

Caroline Kruithoff, Ahmed Gamal, Thomas S. McCormick, Mahmoud A. Ghannoum

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Incidence; Terbinafine; Dermatophytes; Trichophyton; T. rubrum; T. mentagrophytes; T. indotineae; Superficial Fungal Infection

Online: 16 November 2023 (11:22:14 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202311.0380.v1

Fifteen Years after the Definition of Trypanosoma cruzi DTUs: What Have We Learned?

Bianca Zingales, Andréa M Macedo

Subject: Biology And Life Sciences, Other Keywords: Trypanosoma cruzi; Discrete Typing Units; genomics; Chagas disease manifestations; ecoepidemiology

Online: 7 November 2023 (11:40:14 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.0140.v1

Larvicidal Potential of Silver Nanoparticles Synthesized From Ocimum gratissimum Leaf Extracts Against Anopheles Mosquito

Adedamola Sunmonu, Nzube Ekpunobi

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Ocimum gratissimum; silver nano-particle larvicides; Anopheles; Insecticide-resistant mosquito

Online: 4 December 2023 (07:52:48 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.2001.v1

Development of an Eco-Friendly Nanogel Incorporating Pectis brevipedunculata Essential Oil as Larvicidal Agent Against Aedes aegypti

Estela Mesquita Marques, Raiene Lisboa Rocha, Clenilma Marques Brandão, Júlia Karla de Albuquerque Melo Xavier, Marcos Bispo Pinheiro Camara, Caritas de Jesus Silva Mendonça, Roberto Batista de Lima, Melissa Pires Souza, Emmanoel Vilaça Costa, Renato Sonchini Gonçalves

Subject: Chemistry And Materials Science, Nanotechnology Keywords: thermoresponsive nanogel; Pectis brevipendunculata essencial oil; Aedes aegypti

Online: 25 September 2024 (12:10:31 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1116.v1

Characterization of Biofilm Formation by Staphylococcus aureus Depending on NaCl Concentration

Yusuke Iwabuchi, Hitoyuki Kato, Masanori Saito, Hidenobu Senpuku

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: biofilm; membrane vesicle; S. mutans; NaCl; S. aureus

Online: 14 September 2024 (08:44:10 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.0232.v1

Characterization of Qu-aroma of Medium-High Temperature Daqu from Different Production Areas Using Sensory Evaluation, E-nose, and GC-MS/O Analysis

Jie Deng, Jia Zheng, Xing Li, Zhiguo Huang, Dan Huang, Guangbin Ye, Huibo Luo

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Medium-High Temperature Daqu; Qu-aroma; Volatile compounds

Online: 4 September 2024 (03:08:27 CEST)

Show abstract| Download PDF| Share

BACKGROUND: Qu-aroma is a critical quality indicator for medium-high temperature Daqu (MT-Daqu), significantly influencing the aroma profile of strong-aroma Baijiu (SAB). SAB is produced across various regions in China, each with unique environmental conditions and production techniques. These regional differences are likely to cause variations in the Qu-aroma of MT-Daqu. Despite this, comprehensive research on the variations in Qu-aroma across different regions has been lacking. This study aims to address this gap by utilizing Gas Chromatography-Olfactometry-Mass Spectrometry (GC-O-MS) and electronic nose (E-nose) technology, combined with sensory evaluation, to analyze the differences in Qu-aroma characteristics and volatile compound profiles of MT-Daqu from seven distinct production areas.RESULTS: Sensory analysis identified seven broad aroma categories and 25 specific aromas characterizing the Qu-aroma of MT-Daqu samples. Chen aroma and roasted aroma were notably more prevalent in MT-Daqu samples from Sichuan compared to those from other regions (P < 0.05), while the fermentation aroma was less prominent in Sichuan samples. In total, 123 volatile compounds were detected in MT-Daqu, with 46 classified as aroma-active compounds. Among these, 21 aroma-active compounds were identified as markers distinguishing Sichuan MT-Daqu from those produced in non-Sichuan regions, showing significant differences in their concentrations (P < 0.05). Additionally, six major aroma-active compounds-ethyl isovalerate, ethyl heptanoate, hexanal, 1-nonanal, (E)-2,4-decadienal, and 2,3-dimethyl-5-ethylpyrazine-were found to significantly contribute to the aroma differences between Sichuan and non-Sichuan MT-Daqu.CONCLUSION: This study delineates the distinct Qu-aroma characteristics of MT-Daqu from various SAB production areas and identifies the aroma-active compounds responsible for these differences. The insights gained from this research offer valuable guidance for optimizing flavor profiles in MT-Daqu production, potentially enhancing the overall quality and regional distinctiveness of the product.

Preprint ARTICLE | doi:10.20944/preprints202404.1648.v1

Ixodes scapularis Calreticulin Binds Complement Proteins but Does Not Protect Borrelia burgdorferi from Complement Killing

Moiz Ashraf Ansari, Thu-Thuy Nguyen, Klaudia Izabela Kocurek, Tae Heung Kim, Kim Kwon Tae-kwon, Albert Mulenga

Subject: Biology And Life Sciences, Parasitology Keywords: Ixodes scapularis; complement cascade; tick calreticulin; Borrelia burgdorferi

Online: 25 April 2024 (10:05:43 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202404.1094.v1

An Uplifting Avenue upon Mealworm Chitosan for Hemodialysis Application

Maria Martingo, Sara Baptista-Silva, Raquel Mesquita, João Paulo Ferreira, Sandra Borges, Manuela Pintado

Subject: Chemistry And Materials Science, Biomaterials Keywords: Tenebrio molitor; chitosan; bio-based membrane; hemodialysis treatment

Online: 17 April 2024 (14:57:06 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202404.0993.v1

Mycobacterium avium subsp. paratuberculosis in Wild Boar (Sus scrofa) in Portugal

Ana Cristina Matos, Luís Figueira, Maria Helena Martins, Luís Cardoso, Manuela Matos, Maria de Lurdes Pinto, Ana Cláudia Coelho

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Epidemiology; Mycobacterium avium subsp. paratuberculosis; ; wild boar

Online: 16 April 2024 (07:26:01 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1059.v1

Metagenomic Analysis of Viromes of Aedes Mosquitoes across India

Abhranil Gangopadhayya, Kavita Lole, Onkar Ghuge, Ashwini Ramdasi, Asmita Kamble, Diya Roy, Shivani Thakar, Amol Nath, Sudeep AB, Sarah Sabu Cherian

Subject: Biology And Life Sciences, Virology Keywords: Virome; Aedes aegypti; Aedes albopictus; PCLV; CFAV; CfGV

Online: 14 December 2023 (06:38:31 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202404.1112.v1

Effect of Sex, Age and Temperature on the Functional Response of Macrolophus pygmaeus Ramber and Nesidiocoris tenuis Reuter (Heteroptera: Miridae), on Eggs of Tuta absoluta

Eleni Yiacoumi, Nikos A. Kouloussis, Dimitrios S. Koveos

Subject: Biology And Life Sciences, Insect Science Keywords: M. pygmaeus; N. tenuis; Miridae; T.absoluta; functional response; tomato; sex; adult age; temperature; predator

Online: 17 April 2024 (11:57:43 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202401.1280.v1

Burkholderia pseudomallei Complex Subunit and Glycoconjugate Vaccines and Their Potential to Elicit Cross-Protection to Burkholderia cepacia complex.

Alexander J Badten, Alfredo G Torres

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Burkholderia pseudomallei; Burkholderia mallei; Burkholderia cepacia complex; melioidosis; glanders; cystic fibrosis; vaccines; subunit; glycoconjugate

Online: 17 January 2024 (08:35:38 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202408.2124.v1

Effectively Increase the L(+)-Isomer Proportion of Ethyl Lactate in Baijiu by Isolating and Applying L(+)-Lactic Acid-Producing Bacteria

Zhiqiang Ren, Lvchang Liu, Tuoxian Tang, Keyi Huang, Zhiguo Huang

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Strong-aroma Baijiu; L(+)-lactic acid; L(+)-ethyl lactate; Fermented grains; Bacillus licheniformis; Bacillus cereus

Online: 29 August 2024 (08:36:40 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1987.v1

Essential Oils as an Antifungal Alternative to Control Fusarium spp., Penicillium spp., Trichoderma spp. and Aspergillus spp.

Maritza Ruiz-Medina, Jenny Ruales

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: oregano; Origanum; rosemary; Salvia rosmarinus; clove; Syzygium aromaticum; thyme; Thymus; basil; Ocimum basilicum; cinnamon Cinnamomum

Online: 28 August 2024 (10:39:26 CEST)

Show abstract| Download PDF| Share

Essential oils oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus), cinnamon (Cinnamomum verum), basil (Ocimum basilicum), have antifungal properties. This study evaluated its ability to inhibit growth of isolated fungi and purified obtained from the rot of the banana peel, for the purpose of combating or inhibiting fungal growth in bananas (Musa paradisiaca). The methodology consists in preparing the dilutions to inoculate on PDA medium (Potato Dextrose Agar) amended with chloramphenicol to re-inoculate until obtaining the isolated and purified fungi of Trichoderma spp., Aspergillus spp., Penicillium spp. and Fusarium spp., which are characterized macroscopically and microscopically, subsequently, the growth is analyzed in vivo and the inhibition percentage in vitro. The results of the analysis in vivo report that the fungi in order of severity from greatest to least, was Penicillium spp., Trichoderma spp, Fusarium spp. y Aspergillus spp., while that the analysis in vivo reports that the rosemary and the basil does not inhibit fungal growth, while that the clove inhibit a 800 ppm, the cinnamon to 400 ppm and the oregano to 200 ppm to the four fungi. -2077601433541Citation: To be added by editorial staff during production. Academic Editor: Firstname Lastname Received: date Revised: date Accepted: date Published: date Copyright: © 2024 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).00Citation: To be added by editorial staff during production. Academic Editor: Firstname Lastname Received: date Revised: date Accepted: date Published: date Copyright: © 2024 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Preprint ARTICLE | doi:10.20944/preprints202409.2087.v1

Evaluation of Brachypodium spp. System Model Against Fusarium poae

Florencia Arroyo, Mauro Martínez, Agustín Arata, MARIA VIRGINIA MORENO, Marie Dufresne, Sebastian Stenglein, María Inés Dinolfo

Subject: Biology And Life Sciences, Plant Sciences Keywords: plant-pathogen interactions, Fusarium poae, disease severity, Brachypodium spp

Online: 26 September 2024 (07:41:30 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1304.v1

Whole Exome Sequencing of Meningioma in Sudanese Patients

Sawsan Aldeaf, Alsadig Gassoum, Nihad Elsadig, Mohamed Arbab, Muntaser Ebrahim

Subject: Medicine And Pharmacology, Other Keywords: WES; AKT1; CD44; meningioma; candidate genes; NCNS

Online: 18 September 2024 (05:24:59 CEST)

Show abstract| Download PDF| Share

Introduction Meningioma is the second most common primary intracranial tumor of the central nervous system. in Sudan, meningioma is the most common primary brain tumor, among Sudanese patients, Surgical total excision of meningioma offers a better survival, however, Chemotherapy has largely been unsuccessful for meningioma treatment, and therefore, refractory and recurrence meningioma treated with palliative surgery and radiotherapy. Monosomy of chromosome 22 is the utmost common genetic alteration among meningioma and many genes were investigated and described in the development of this tumor of which , NF2 hSNF5/INI1gene, P53. In addition to this, Molecular signal pathways in meningioma, and that contribute to the survival, proliferation, self-renewal, and differentiation properties of normal stem cells which are abnormally activated and nominated as cancerous stem cell (CSCs). Objectives 1/ To identify the possible pathway of candidate genes in both setting and whole genes hypothesis free approach 2/ To assess the presence of mutations in candidate genes among histological subtypes of meningioma in Sudan. Material and methods This is a prospective cross-sectional study done at the National Center of Neurological Sciences (NCNS) Khartoum, from 2018 to 2021. The study included all cerebral tumors that were radio- logically diagnosed preoperatively as meningioma. Ethical approval was taken from Institute of Endemic disease, Khartoum University, and written consent was obtained Three DNA extracts were randomly selected from 20 meningioma tissue samples and processed for WES. All genes harboring exonic and splice site variations stratified into SNVs and INDELs sets were prepared. The 1000G online database was used to explore novel mutations per 3 samples. SIFT and PolyPhen-2 programs were used to predict possible impact of amino acids substitution. To address meticulous genes interaction and particular centrality, all shared genes within the class INDEL and SNVs were used as input on Network Analysis to predict protein- protein interaction and functional gene ontology. Finally, shared mutations within genes of centrality were detected via Sanger sequencing and mutations were correlated between histological sub-types and recurrenceThree non meningioma brain tumor used as control. Results WES analysis revealed high number for mutational signature within 2 classes (INDEL and SNVs) per 3 samples, the findings of 1000 genome online database showed number of novel mutations; 5618 in fibrous , 6032 in meningothelial while in recurrent fibrous sample 5930. Network analysis of shared genes per each class (INDEL, SNVs), showed UBC is the most protein of remarkable degree centrality over thousand of genes. Whereas, all shared genes per 3 samples within the 2 classes (INDEL and SNVs) revealed Akt1 with the highest degree of centrality and betweenness. Akt1 rs17846829was detected in 20% of the samples, while CD44 stemness gene revealed mutation A>G (rs9666607) in all meningioma samples (100%), this mutation was associated with 60% of the recurrence. Conclusions Nevertheless, the present study, highlight how such complex molecular processes might contribute to tumorogenesis, in attempt to shed light over thousands of genes and multifaceted network analysis rather than a solitary gene which is challenging question.

Preprint SHORT NOTE | doi:10.20944/preprints202408.1662.v1

Recent Advances in Molecular Diagnostic Methods for Foodborne Pathogens with a Focus on Listeria monocytogenes and Salmonella spp

Josué J. Da Silva

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Molecular Diagnostics; Foodborne Pathogens; Listeria monocytogenes; Salmonella Detection; NGS

Online: 23 August 2024 (00:11:54 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202408.1120.v1

Complete Genome Sequence of the Butirosin-producing Bacillus vitellinus NBRC 13296 and its reclassification to Paenibacillus chitinolyticus

Kyung-A Hyun, Seung-Young Kim, Kyung-Hwan Boo, Won-Jae Chi, Chang-Gu Hyun

Subject: Biology And Life Sciences, Life Sciences Keywords: Bacillus vitellinus NBRC 13296; butirosin BGC, Paenibacillus chitinolyticus; reclassification

Online: 15 August 2024 (06:28:04 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Butirosins are naturally occurring aminoglycoside (AG) antibiotics featuring a 4,5-disubstituted 2-deoxystreptamine (2-DOS) with a (2S)-4-amino-2-hydroxybutyrate (AHBA) side chain. This side chain has been shown to confer resistance against AG modifying enzymes, leading to ongoing studies on the butirosin biosynthetic pathway and the corresponding enzymes. Butirosin is produced by Niallia (formerly Bacillus) circulans and Bacillus vitellinus, with most research focused on the former strain. To date, no whole genome analysis has been performed on B. vitellinus. In this study, we sequenced the complete genome of B. vitellinus NBRC 13296 and performed a comparative analysis of different butirosin biosyntheric gene clusters (BGCs), including those from N. circulans. The complete genome of B. vitellinus NBRC 13296 comprises a 6,331,192 base circlular chromosome with a GC content of 52.68%. The annotation revealed the presence of 5,605 CDSs, 70 tRNA genes, 30 rRNA genes, and 3 ncRNA genes in NBRC 13296. The highest dDDH and ANI values between NBRC 13296 and the most closely related type strain, Paenibacillus chitinolyticus KCCM 41400, were 97.8% and 98.66%, respectively. Based on these genome-based comparative analyses, we propose reclassifying B. vitellinus NBRC 13296 as P. chitinolyticus. Genome mining revealed 18 gene clusters encoding the biosynthesis of diverse secondary metabolites in the genome of B. vitellinus NBRC 13296, indicating the enormous biosynthetic potential of this strain. The predicted structural diversity of secondary metabolites includes aminoglycoside, PKS, NRPS, PKS-NRPS hybrids, metallophores, phosphonates, terpenes, β-lactones, and RiPP peptides. We then comparatively characterized butorosin BGCs previously studied in several N. circulans strains. Additionally, comparative genome analysis revealed complete butirosin BGCs identified from P. chitinolyticus KCCM 41400, P. chitinolyticus NRRL B-23119, P. chitinolyticus NRRL B-23120, P. chitinolyticus B-14908, P. chitinolyticus YSY-3.1, P. chitinolyticus JMW06, Paenibacillus sp. GbtcB18, Paenibacillus sp. HGH0039, Paenibacillus sp. MZ04-78.2. Finally, we identified the core region consisting of BtrS, BtrN, BtrM, BtrL, BtrA, BtrB, BtrC, BtrD, BtrD, BtrE, BtrF, BtrG, BtrH, BtrI, BtrI, BtrJ, BtrK, BtrO, BtrP, and BtrV, followed by an upstream region organizing BtrQ, BtrW, BtrX, BtrY, and BtrZ in the same transcriptional direction and sequential genetic arrangement, and a downstream region organizing various proteins based on BtrT, BtrR2, BtrU, and BtrR1. Our study provided insights into the reclassification of B. vitellinus NBRC 13296 to P. chitinolyticus and suggested the need for continued studies on butirosin biosynthesis from an enzymatic perspective.

Preprint ARTICLE | doi:10.20944/preprints202407.1518.v1

Whole Genome Sequencing of the Entomopathogenic Fungus Lecanicillium lecanii, KMZW-1 and Its Efficacy against Bactrocera dorsalis

Junfu Yu, Mehboob Hussain, Mingqi Wu, Chunlan Shi, Shini Li, Yuanxia Ji, Sikandar Hussain, Deqiang Qin, Chun Xiao, Guoxing Wu

Subject: Biology And Life Sciences, Insect Science Keywords: Lecanicillium lecanii; whole genome sequencing; entomopathogenic fungus; Bactrocera dorsalis

Online: 19 July 2024 (02:30:15 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0085.v1

Proteome Dynamics of Arabidopsis thaliana Under Moderate Heat Stress

Kai-Ting Fan, Yuan Xu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: crop resilience; Arabidopsis thaliana; heat stress; proteomics; thermal adaptation

Online: 2 May 2024 (12:04:12 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202403.1866.v1

Proximate Causes of Ageing Are Likely Evolutionarily Bound and Appropriate Countermeasure Design Requires New Approaches

Luke Slade, Timothy Etheridge, Nathaniel Szewczyk

Subject: Biology And Life Sciences, Aging Keywords: Hyperfunction; C. elegans; ageing; gene optimisation; healthspan

Online: 29 March 2024 (15:55:49 CET)

Show abstract| Download PDF| Share

Understanding mechanisms of ageing remains a complex challenge for biogerontologists, but recent adaptations of evolutionary ageing theories offer a compelling lens in which to view both age-related molecular and physiological deterioration. Ageing is commonly associated with a progressive loss of biochemical processes and degeneration of molecular material, however, the mechanisms of diminishing function are not simply a result of aberrant expression of these pathways. Natural selection pressures are at their highest in youthful periods to select genes advantageous to maximising reproductive capacity. After sexual maturation, selective pressure diminishes, subjecting individuals to maladaptive pleiotropic gene function once beneficial for developmental growth, but pathogenic later in life. Due to this selective ‘shadowing’ in ageing, mechanisms to counter hyperfunctional genes are unlikely to evolve within a given species. These genetic ‘run-on’ programmes are not dysfunctional per se and, in fact, remain highly robust. Thus, interventions capable of optimising gene function during the ageing process represent as attractive therapeutic modalities. Caenorhabditis elegans offers a unique model to explore high-throughput screening of interventions to combat hyperfunctional pathways involved in ageing. However, the majority of research to date employ whole-life vs late-life pathway manipulation of developmental/growth-related systems to claim ageing attenuation, despite significant impairments in early-life fitness and discordance with healthspan. Here, we discuss data supporting hyperfunctional changes at a global molecular and functional level in C. elegans, and how classical lifespan-extension mutants alter these dynamics. We later discuss the relevance of such mutant models for exploring mechanisms of ageing, highlighting that post-reproductive gene manipulation represents a far more translatable approach for C. elegans research not routed within pleiotropic constraints. More so, lifespan has become a routine readout for quantifying ‘ageing’, however, we make the argument that a compression of gerospan vs lifespan extension is a more meaningful goal for anti-ageing regimes with current knowledge. Similarly, mechanisms governing lifespan are distinct from those regulating health, and slowing senescent pathology accumulation appears more applicable for the abstraction to human ageing.

Preprint SHORT NOTE | doi:10.20944/preprints202312.1904.v2

Living on the Edge: Navigating the Complex Landscape of Borderline Personality Disorder

Sharif Mohammed Sadat, Dev Desai, Maria Eleni Malafi

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: Borderline personality Disorder; instability; Impulsive; Stigma; Psychiatry
;

Online: 12 February 2024 (11:08:01 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202312.0039.v1

The Crayfish Plague Pathogen Aphanomyces astaci in Ireland.

Daniel J. Brady, Rossa Meade, Julian D. Reynolds, Andreas Vilcinskas, Kathrin Theissinger

Subject: Biology And Life Sciences, Aquatic Science Keywords: crayfish plague; Aphanomyces astaci; Austropotamobius pallipes; invasive species; Ireland

Online: 1 December 2023 (05:31:53 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1489.v1

In-Vitro Activity of N, N-diethyldithiocarbamate against Ureaplasma urealyticum and Ureaplasma parvum Isolated from Women with Infections of the Urogenital Tract

Małgorzata Biernat-Sudolska, Danuta Rojek-Zakrzewska, Kamil Drożdż, Anna Bilska-Wilkosz

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Ureaplasma urealyticum; Ureaplasma parvum; N,N-diethyldithiocarbamate; disulfiram; urease

Online: 23 November 2023 (07:38:15 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1413.v1

Characterization of O26 Enteropathogenic E. coli and Its Impli-Cation for Vaccine Development

Thiago Jordão da Silva Lemos, Herbert Guimarães de Sousa Silva, José Osvaldo Previato, Lucia Mendonça-Previato, Elisangela Oliveira de Freita, Angela Silva Barbosa, Marcia Regina Franzolin, Luis Fernando dos Santos, Bruna de Sousa Melo, Geovana Ferreira dos Anjos, Renata Hiromi Nakagima Gonçalves, Marta de Oliveira Domingos

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: E. coli 1; polysaccharide O26 2; vaccine 3; enteropathogenic E. coli; EPEC; shiga toxin-producing E. coli; STEC; enterohemorrhagic E. coli; EHEC

Online: 19 December 2023 (07:55:19 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1855.v1

Antimicrobial Susceptibility of Uropathogenic Escherichia coli with Analysis of Impact of Socio-Economic Factors in Low, Medium and High-Income Countries

Meher Rizvi, Maria Khan, Amina AL Jardani, Ujjala Ghoshal, Zaaima AL Jabri, Mohammad Ahanjan, Azza AL Mamari, Nawal AL Shizawi, Hiba Sami, Abdullah Balkhair, Adila Shaukat, Alwarith Nasser Salem Alkharusi, Abdulrahman Almahrouqi, Afreenish Hassan, Ajay Kumar, Amal Malehi, Amina Gul, Ashima Singh, Asma Said Hamed AL Shidhani, Areena Hoda Siddiqui, Aruna Poojary, Azza AL Rashdi, Bradley Langford, Dmytro Stepanskyi, Abbas Dolatabadi, Aisha Al Mufraji AL Mufraji, Elham Said Ahmed AL Risi, Garima Kapoor, Isabella Princess, Hatim Ali Eltahir, Hilal Al Sidairi, Kaitlyn Dougherty, Katia Iskandar, Ken Masters, Ibrahim Khalaf Hamdan AL Busaidi, Laila AL Yazidi, Mahfuza Nasrin, Mahmood Salim AL Subhi, Mallika Sengupta Mallika Sengupta, Manisha Khandait, Nada Khalfan AL Tamtami, Nada Khafan Alsiyabi, Nawal AL Kindi, Nazla Musthafa Luthfee, Neha Shreshtha, Nihal Mohamed Amur AL Riyami, Noora Hilal Ali AL Busaidi, Nupur Goel, Oksana Ishchenko, Omnia Mohamed Elnabawy Ahmed Taher, Pragnya Paramita Jena, Rajni Ekadashi, Razan Zadjali, Reba Kanungo, Sara Abolghasemi, Salima Al-maqbali, Sanjay Jaju, Sayantan Banerjee, Shadma Yaqoob, Shahnaz Sali, Shafqat Husnain Khan, Shalini Malhotra, Stephen Hughes, Sundas Shaukat, Syed Mohammed Atif, Vrushali Vishwas Patwardhan, Victorien Dougnon, Wahid Khan, Walid Wali, Zakariya AL Muharrmi, Zeeshana Basit, Jasashree Choudhury, Rachita Pravalina, Sanjeev Singh, Sarman Singh, Neelam Taneja, Raman Sardana, Pawan Kapoor, Rajeev Soman, Rashid Al Abri

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: community acquired UTI; Escherichia coli; antimicrobial susceptibility; Indian subcontinent; MENA; GDP; temperature

Online: 24 September 2024 (12:32:05 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202407.1954.v1

Antiparasitary Potential Of Natural And Semi-Synthetic Labdane Diterpenes

Aline Nazaré Silva Parra, Ana Carolina Ferreira Soares Rocha, Julian Carlos da Silva Pavan, Analuz da Silva Machado, Vinícius José Miranda Rodrigues, Daiane Albino dos Santos, Lizandra Guidi Magalhães, Sérgio de Albuquerque, Vladimir Constantino Gomes Heleno

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: Leishmaniasis; Chagas disease; Copalic acid; Structural Modification; Trypanocidal and Leishmanicidal activities

Online: 24 July 2024 (17:45:24 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202406.0369.v1

Vitis rotundifolia Genes Introgressed with RUN1 and RPV1: Poor Recombination and Impact on v. vinifera Berry Transcriptome

Mengyao SHI, Stefania Savoi, Gautier Sarah, Alexandre Soriano, Audrey Weber, Laurent Torregrosa, Charles Romieu

Subject: Biology And Life Sciences, Plant Sciences Keywords: Hybrids; Fruit development; V. rotundifolia; V. vinifera; Transcriptome; Resistance genes

Online: 6 June 2024 (11:42:44 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Thanks to several Vitis vinifera backcrosses with an initial V. vinifera L. × V. rotundifolia (previously Muscadinia rotundifolia) interspecific cross, the MrRUN1/MrRPV1 locus (resistance to downy and powdery mildews) was introgressed in genotypes phenotypically close to V. vinifera varieties. To check the consequences of introgressing parts of the V. rotundifolia genome on gene expression during fruit development, we conducted a comparative RNA-seq study on single berries from different V. vinifera cultivars and V. vinifera × V. rotundifolia hybrids, including ‘G5’ and two derivative microvine lines, ‘MV102’ (resistant) and ‘MV32’ (susceptible) segregating for the MrRUN1/RPV1 locus. RNA-Seq profiles were analyzed on a comprehensive set of single berries from the end of the herbaceous plateau to the ripe stage. Pair-end reads were aligned both on V. vinifera PN40024.v4 reference genome, V. rotundifolia cv ‘Trayshed’ and cv ‘Carlos’, and to the few resistance genes from the original V. rotundifolia cv ‘52’ parent available at NCBI. Weighted Gene Co-expression Network Analysis (WGCNA) led to classifying the differentially expressed genes into 15 modules either preferentially correlated with resistance or berry phenology and composition. Resistance positively correlated transcripts predominantly mapped on the 4-5 Mb distal region of V. rotundifolia chromosome 12 beginning with the MrRUN1/MrRPV1 locus, while the negatively correlated ones mapped on the orthologous V. vinifera region, showing this large extremity of LG12 remained recalcitrant to internal recombination during the successive backcrosses. Some constitutively expressed V. rotundifolia genes were also observed at lower density outside this region. Genes overexpressed in developing berries from resistant accessions, either introgressed from V. rotundifolia, or triggered by these in the vinifera genome, spanned various functional groups, encompassing calcium signal transduction, hormone signaling, transcription factors, plant–pathogen-associated interactions, disease resistance proteins, ROS and phenylpropanoid biosynthesis. This transcriptomic insight provides a foundation for understanding the disease resistance inherent in these hybrid cultivars and suggests a constitutive expression of NIR NBS LRR triggering calcium signaling. Moreover, these results illustrate the magnitude of transcriptomic changes caused by the introgressed V. rotundifolia background in backcrossed hybrids, on a large number of functions largely exceeding the ones constitutively expressed in single resistant gene transformants.

Preprint ARTICLE | doi:10.20944/preprints202403.0609.v1

The Optimal Concentrations of Ethanolic-Extracted Propolis for a Reduction of Nosema ceranae Infection in Western Honey Bees, Apis mellifera

Sanchai Naree, James D. Ellis, Guntima Suwannapong

Subject: Biology And Life Sciences, Insect Science Keywords: Apis mellifera; ethanolic extracted propolis; infectivity; infection rate; Nosema ceranae

Online: 11 March 2024 (21:47:59 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.0353.v1

Quality Characteristics and Antioxidant Activity of the Korean Traditional Rice-Oat Wine “Makgeolli” Supplemented with Codium fragile, a Green Seaweed

Eun Hee Park, Eun Bi Jeon, Shin Young Park

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Makgeolli; Rice-Oat Mixture; Codium fragile; Antioxidant activity; Sensory properties

Online: 4 January 2024 (07:16:55 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.0085.v1

Megaselia scalaris and Senotainia tricuspis Infesting Apis mellifera: Genotyping, Detection by Quantitative PCR, and Involvement in the Transmission of Microbial Pathogens

Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti

Subject: Biology And Life Sciences, Insect Science Keywords: Parasitoid flies of Apis mellifera; Megaselia scalaris; Senotainia tricuspis; qPCR detection; occurrence in apiaries; genotyping; pathogen transmission

Online: 2 September 2024 (16:30:19 CEST)

Show abstract| Download PDF| Share

Search Results

530 articles found